| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 3,853,312 | T→C | intergenic (‑324/‑241) | ivbL ← / → tisB | ilvB operon leader peptide/toxic membrane persister formation peptide, LexA‑regulated |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,853,312 | 0 | T | C | 98.3% | 194.4 / ‑4.2 | 58 | intergenic (‑324/‑241) | ivbL/tisB | ilvB operon leader peptide/toxic membrane persister formation peptide, LexA‑regulated |
| Reads supporting (aligned to +/- strand): ref base T (0/1); new base C (27/30); total (27/31) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.88e-01 | |||||||||||
GCAGAACGCGCAATTTCGGCACGAATTTTGACGTATTTAGTGCATAGTTGAGTATCGATCACAGTTTGCGTTTTGTCCAAATATTACTGTTTATTTATACAGTAAACTTCTATAATATCACTGTACGCAATGTGTTATGCGGGGGCCGCATCGTTACCCGGCGCACTAAGTCCTGGCTGAAACGGGTGGTGCCG > NC_000913/3853215‑3853408 | gcAGAACGCGCAATTTCGGCACGAATTTTGACGTATTTAGTGCATAGTTGAGTATCGATCACAGTTTGCGTTTTGTCCAAATATTACTGTTTATTTacac < 2:962970/100‑1 (MQ=255) aGAACGCGCAATTTCGGCACGAATTTTGACGTATTTAGTGCATAGTTGAGTATCGATCACAGTTTGCGTTTTGTCCAAATATTACTGTTTATTTACACAg < 1:560944/100‑1 (MQ=255) gAACGCGCAATTTCGGCACGAATTTTGACGTATTTAGTGCATAGTTGAGTATCGATCACAGTTTGCGTTTTGTCCAAATATTACTGTTTATTTACACAGt < 2:28901/100‑1 (MQ=255) cgcgCAATTTCGGCACGAATTTTGACGTATTTAGTGCATAGTTGAGTATCGATCACAGTTTGCGTTTTGTCCAAATATTACTGTTTATTTACACAGt < 2:29013/97‑1 (MQ=255) gcgcAATTTCGGCACGAATTTTGACGTATTTAGTGCATAGTTGAGTATCGATCACAGTTTGCGTTTTGTCCAAATATTACTGTTTATTTACACAGTAAAc < 1:922639/100‑1 (MQ=255) ttccgatctggCACGAATTTTGACGTATTTAGTGCATAGTTGAGTATCGATCACAGTTTGCGTTTTGTCCAAATATTACTGTTTATTTACACAGTAAACt < 1:1154882/91‑1 (MQ=255) cgcAATTTCGGCACGAATTTTGACGTATTTAGTGCATAGTTGAGTATCGATCACAGTTTGCGTTTTGTCCAAATATTACTGTTTATTTACACAGTAAACt > 1:668311/1‑100 (MQ=255) cgcAATTTCGGCACGAATTTTGACGTATTTAGTGCATAGTTGAGTATCGATCACAGTTTGCGTTTTGTCCAAATATTACTGTTTATTTACACAGTAAACt < 2:429420/100‑1 (MQ=255) ttCGGCACGAATTTTTACTTATTTAGTGCATAGTTTAGTATCGATCACAGTTTGCGTTTTGTCCAAATATTACTGTTTATTTACACAGTAAACTTCtata < 2:935164/100‑1 (MQ=255) cGGCACGAATTTTGACGTATTTAGTGCATAGTTGAGTATCGATCACAGTTTGCGTTTTGTCCAAATATTACTGTTTATTTACACATTAAACTTCTataat > 1:1530073/1‑100 (MQ=255) ggCACGAATTTTGACGTATTTAGTGCATAGTTGAGTATCGATCACAGTTTGCGTTTTGTCCAAATATTACTGTTTATTTACACAGTAAACtagatcggaa > 2:1154875/1‑91 (MQ=255) ggCACGAATTTTGACGTATTTAGTGCATAGTTGAGTATCGATCACAGTTTGCGTTTTGTCCAAATATTACTGTTTATTTACACAGTAAACTTCTataata < 1:1047124/100‑1 (MQ=255) cGAATTTTGACGTATTTAGTGCATAGTTGAGTATCGATCACAGTTTGCGTTTTGTCCAAATATTACTGTTTATTTACACAGTAAACTTCTATAATATCAc > 2:352542/1‑100 (MQ=255) cGAATTTTGACGTATTTAGTGCATAGTTGAGTATCGATCACAGTTTGCGTTTTGTCCAAATATTACTGTTTATTTACACAGTAAACTTCTATAATATCAc < 2:794204/100‑1 (MQ=255) cGAATTTTGACGTATTTAGTGCATAGTTGAGTATCGATCACAGTTTGCGTTTTGTCCAAATATTACTGTTTATTTACACAGTAAACTTCTATAATATCAc > 1:755883/1‑100 (MQ=255) tGACGTATTTAGTGCATAGTTGAGTATCGATCACAGTTTGCGTTTTGTCCAAATATTACTGTTTATTTACACAGTAAACTTCTATAATATCACTGTACGc < 2:322743/100‑1 (MQ=255) tGACGTATTTAGTGCATAGTTGAGTATCGATCACAGTTTGCGTTTTGTCCAAATATTACTGTTTATTTACACAGTAAACTTCTATAATATCACTGTACGc < 2:386775/100‑1 (MQ=255) tGACGTATTTAGTACATAGTTGAGTATCGATCACAGTTGGCGTTTTGTCCAAATATTACTGTTTATTTACACAGTAAACTTCTATAATATCACTGTACGc > 2:968475/1‑100 (MQ=255) aCGTATTTAGTGCATAGTTGAGTATCGATCACAGTTTGCGTTTTGTCCAAATATTACTGTTTATTTACACAGTAAACTTCTATAATATCACTGTACGCaa > 1:8156/1‑100 (MQ=255) tttAGTGCATAGTTGAGTATCGATCACAGTTTGCGTTTTGTCCAAATATTACTGTTTATTTACCCAGTAAACTTCTATAATATCACTGTACGCAATgtgt > 1:191833/1‑100 (MQ=255) tttAGTGCATAGTTGAGTATCGATCACAGTTTGCGTTTTGTCCAAATATTACTGTTTATTTACACAGTAAACTTCTATAATATCACTGTACGCAATgtgt < 2:668308/100‑1 (MQ=255) ttAGTGCATAGTTGAGTATCGATCACAGTTTGCGTTTTGTCCAAATATTACTGTTTATTTACACAGTAAACTTCTATAATATCACTGTACGCAATGTGtt > 2:10397/1‑100 (MQ=255) aGTGCATAGTTGAGTATCGATCACAGTTTGCGTTTTGTCCAAATATTACTGTTTATTTACACAGTAAACTTCTATAATATCACTGTACGCAATGTGTTAt < 2:313741/100‑1 (MQ=255) aGTGCATAGTTGAGTATCGATCACAGTTTGCGTTTTGTCCAAATATTACTGTTTATTTACACAGTAAACTTCTATAATATCACTGTACGCAATGTGTTAt < 2:77259/100‑1 (MQ=255) gCATAGTTGAGTATCGATCACAGTTTGCGTTTTGTCCAAATATTACTGTTTATTTACACAGTAAACTTCTATAATATCACTGTACGCAATGTGTTATGc > 2:40277/1‑99 (MQ=255) gCATAGTTGAGTATCGATCACAGTTTGCGTTTTGTCCAAATATTACTGTTTATTTACACAGTAAACTTCTATAATATCACTGTACGCAATGTGTTATGCg > 2:1137514/1‑100 (MQ=255) cATAGTTGAGTATCGATCACAGTTTGCGTTTTGTCCAAATATTACTGTTTATTTACACAGTAAACTTCTATAATATCACTGTACGCAATGTGTTATGCgg < 1:325641/100‑1 (MQ=255) tAGTTGAGTATCGATCACAGTTTGCGTTTTGTCCAAATATTACTGTTTATTTACACAGTAAACTTCTATAATATCACTGTACGCAATGTGTTATGCgggg > 2:230990/1‑100 (MQ=255) aGTTGAGTATCGATAACAGTTTGCGTTTTGTCCAAATATTACTGTTTATTTACACAGTAAACTTCTATAATATCGCTGTACGCAATGTGTTATGCggggg < 2:897634/100‑1 (MQ=255) gTTGAGTATCGATCACAGTTTGCGTTTTGTCCAAATATTACTGTTTATTTACACAGTAAACTTCTATAATATCACTGTACGCAATGTGTTATGCGGGGGc > 2:1041112/1‑100 (MQ=255) tGAGTATCGATCACAGTTTGCGTTTTGTCCAAATATTACTGTTTATTTACACAGTAAACTTCTATAATATCACTGTACGCAATGTGTTATGCGGGGGCCg < 2:332703/100‑1 (MQ=255) tGAGTATCGATCACAGTTTGCGTTTTGTCCAAATATTACTGTTTATTTACACAGTAAACTTCTATAATATCACTGTACGCAATGTGTTATGCGGGGGCCg > 2:656122/1‑100 (MQ=255) gAGTATCGATCACAGTTTGCGTTTTGTCCAAATATTACTGTTTATTTACACAGTAAACTTCTATAATATCACTGTACGCAATGTGTTATGCGGGGGCCGc < 1:1215983/100‑1 (MQ=255) aGTATCGATCACAGTTTGCGTTTTGTCCAAATATTACTGTTTATTTACACAGTAAACTTCTATAATATCACTGTACGCAATGTGTTATGCGGGGGCCGCa < 2:316773/100‑1 (MQ=255) aGTATCGATCACAGTTTGCGTTTTGTCCAAATATTACTGTTTATTTACACAGTAAACTTCTATAATATCACTGTACGCAATGTGTTATGCGGGGGCCGCa < 1:1085901/100‑1 (MQ=255) aGTATAGATCACAGTTTGCGTTTTGTCCAAATATTACTGTTTATTTACACAGTAAACTTCTATAATATCACTGTACGCAATGTGTTATGCGGGGGCCgaa > 2:315936/1‑98 (MQ=255) cGATCACAGTTTGCGTTTTGTCCAAATATTACTGTTTATTTACACAGTAAACTTCTATAATATCACTGTACGCAATGTGTTATGCGGGGGCCGCATCGtt < 1:10397/100‑1 (MQ=255) gATCACAGTTTGCGTTTTGTCCAAATATTACTGTTTATTTACACAGTAAACTTCTATAATATCACTGTACGCAATGTGTTATGCGGGGGCCGCATCGTTa > 2:112349/1‑100 (MQ=255) tCACAGTTTGCGTTTTGTCCAAATATTACTGTTTATTTACACAGTAAACTTCTATAATATCACTGTACGCAATGTGTTATGCGGGGGCCGCATCGTTAcc < 2:773787/100‑1 (MQ=255) cacaGTTTGCGTTTTGTCCAAATATTACTGTTTATTTACACAGTAAACTTCTATAATATCACTGTACGCAATGTGTTATGCGGGGGCCGCATCGTTAccc < 1:612854/100‑1 (MQ=255) ttGCGTTTTGTCCAAATATTACTGTTTATTTACACAGTAAACTTCTATAATATCACTGTACGCAATGTGTTATGCGGGGGCCGCATCGTTACCCGGCGCa < 2:768306/100‑1 (MQ=255) tGCGTTTTGTCCAAATATTACTGTTTATTTACACAGTAAACTTCTATAATATCACTGTACGCAATGTGTTATGCGGGGGCCGCATCGTTACCCGGCGCAc > 2:527165/1‑100 (MQ=255) gCGTTTTGTCCAAATATTACTGTTTATTTACACAGTAAACTTCTATAATATCACTGTACGCAATGTGTTATGCGGGGGCCGCATCGTTACCCGGCGCACt > 2:1211988/1‑100 (MQ=255) tttGTCCAAATATTACTGTTTATTTACACAGTAAACTTCTATAATATCACTGTACGCAATGTGTTATGCGGGGGCCGCATCGTTACCCGGCGCACTAAGt < 1:602589/100‑1 (MQ=255) tttGTCCAAATATTACTGTTTATTTACACAGTAAACTTCTATAATATCACTGTACGCAATGTGTTATGCGGGGGCCGCATCGTTACCCGGCGCACTAAGt < 1:902801/100‑1 (MQ=255) tGTCCAAATATTACTGTTTATTTACACAGTAAACTTCTATAATATCACTGTACGCAATGTGTTATGCGGGGGCCGCATCGTTACCCGGCGCACTAAGTcc > 2:516133/1‑100 (MQ=255) tGTCCAAATATTACTGTTTATTTACACAGTAAACTTCTATAATATCACTGTACGCAATGTGTTATGCGGGGGCCGCATCGTTACCCGGCGCACTAAGTcc > 2:1132531/1‑100 (MQ=255) ccAAATATTACTGTTTATTTATACAGTAAACTTCTATAATATCACTGTACGCAATGTGTTATGCGGGGGCCGCATCGTTACCCGGCGCACTAAGTCCTgg < 1:1129967/100‑1 (MQ=255) cAAATATTACTGTTTATTTACACAGTAAACTTCTATAATATCACTGTACGCAATGTGTTATGCGGGGGCCGCATCGTTACCCGGCGCACTAAGTCCTGGc > 2:1554132/1‑100 (MQ=255) cAAATATTACTGTATATTTACACAGTCAACTTCTATAATATCACTGTACGCAATGTGTTATGCGGGGGCCGCATCGTTACCCGGCGCACTAAGTACTGGc > 2:936563/1‑100 (MQ=255) atTACTGTTTATTTACACAGTAAACTTCTATAATATCACTGTACGCAATGTGTTATGCGGGGGCCGCATCGTTACCCGGCGCACTAAGTCCTGGCTGaaa < 1:215403/100‑1 (MQ=255) aCTGTTTATTTACACAGTAAACTTCTATAATATCACTGTACGCAATGTGTTATGCGGGGGCCGCATCGTTACCCGGCGCACTAAGTCCTGGCTGAAACgg > 1:1300181/1‑100 (MQ=255) aCTGTTTATTTACACAGTAAACTTCTATAATATCACTGTACGCAATGTGTTATGCGGGGGCCGCATCGTTACCCGGCGCACTAAGTCCTGGCTGAAACgg > 2:907298/1‑100 (MQ=255) cTGTTTATTTACACAGTAAACTTCTATAATATCACTGTACGCAATGTGTTATGCGGGGGCCGCATCGTTACCCGGCGCACTAAGTCCTGGCTGAAACggg < 2:35335/100‑1 (MQ=255) tGTTTATTTACACAGTAAACTTCTATAATATCACTGTACGCAATGTGTTATGCGGGGGCCGCATCGTTACCCGGCGCACTAAGTCCTGGCTGAAACGggt < 1:1379775/100‑1 (MQ=255) gTTTATTTACACAGTAAACTTCTATAATATCACTGTACGCAATGTGTTATGCGGGGGCCGCATCGTTACCCGGCGCACTAAGTCCTGGCTGAAACGggtg < 2:3806/100‑1 (MQ=255) gTTTATTTACACAGTAAACTTCTATAATATCACTGTACGCAATGTGTTATGCGGGGGCCGCATCGTTACCCGGCGCACTAAGTCCTGGCTGAAACGggtg > 2:116946/1‑100 (MQ=255) gTTTATTTACACAGTAAACTTCTATAATATCACTGTACGCAATGTGTTATGCGGGGGCCGCATCGTTACCCGGCGCACTAAGTCCTGGCTGAAACGggtg > 1:1132281/1‑100 (MQ=255) gTTTATTTACACAGTAAACTTCTATAATATCACTGTACGCAATGTGTTATGCGGGGGCCGCATCGTTACCCGGCGCACTAAGTCCTGGCTGAAACGggtg > 1:1453754/1‑100 (MQ=255) atttacACAGTAAACTTCTATAATATCACTGTACGCAATGTGTTATGCGGGGGCCGCATCGTTACCCGGCGCACTAAGTCCTGGCTGAAACGGGTGGTGc < 1:1380456/100‑1 (MQ=255) tttacACAGTAAACTTCTATAATATCACTGTACGCAATGTGTTATGCGGGGGCCGCATCGTTACCCGGCGCACTAAGTCCTGGCTGAAACGGGTGGTGcc > 2:616255/1‑100 (MQ=255) tttacACAGTAAACTTCTATAATATCACTGTACGCAATGTGTTATGCGGGGGCCGCATCGTTACCCGGCGCACTAAGTCCTGGCTGAAACGGGTGGTGcc > 1:669157/1‑100 (MQ=255) ttacACAGTAAACTTCTATAATATCACTGTACGCAATGTGTTATGCGGGGGCCGCATCGTTACCCGGCGCACTAAGTCCTGGCTGAAACGGGTGGTGCCg > 2:1348362/1‑100 (MQ=255) | GCAGAACGCGCAATTTCGGCACGAATTTTGACGTATTTAGTGCATAGTTGAGTATCGATCACAGTTTGCGTTTTGTCCAAATATTACTGTTTATTTATACAGTAAACTTCTATAATATCACTGTACGCAATGTGTTATGCGGGGGCCGCATCGTTACCCGGCGCACTAAGTCCTGGCTGAAACGGGTGGTGCCG > NC_000913/3853215‑3853408 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |