| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 409,322 | G→T | A233E (GCG→GAG) | rdgC ← | nucleoid‑associated ssDNA and dsDNA binding protein; competitive inhibitor of RecA function |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 409,322 | 0 | G | T | 96.2% | 171.4 / ‑3.1 | 52 | A233E (GCG→GAG) | rdgC | nucleoid‑associated ssDNA and dsDNA binding protein; competitive inhibitor of RecA function |
| Reads supporting (aligned to +/- strand): ref base G (2/0); new base T (25/25); total (27/25) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.91e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TCTTCGTTTTGATCGCGCAGCTCGTCGCAGAACTTCAGACGCTTGAGCGAACCATCGTCGCACATCACAAACTGAATGCGCTGCTGCCAGTCGAGCGCCAGTTTAGTCACCACTTTTCCGGCTTCAATGTGATTGGTGATCTCTTCGCTGGTCAGATCTTGTTTCTTCGCGCGGATCACGCCGCCATCTTCCA > NC_000913/409226‑409418 | tCTTCGTTTTGATCGCGCAGCTCGTCGCAGAACTTCAGACGCTTGAGCGAACCATCGTCGCACATCACAAACTGAATGCGCTGCTGCCAGTCGAGCTCCa < 2:222824/100‑1 (MQ=255) cTTCGTTTTGATCGCGCAGCTCGTCGCAGAACTTCAGACGCTTGAGCGTACCCTCGTCGCACATTACACACTGAATGCGCTGCTGCCAGTCGAGCGCCAg > 2:43503/1‑100 (MQ=255) cTTCGTTTTGATCGCGCAGCTCGTCGCAGAACTTCAGACGCTTGAGCGAACCATCGTCGCACATCACAAACTGAATGCGCTGCTGCCAGTCGAGCTCCAg > 2:978540/1‑100 (MQ=255) cTTCGTTTTGATCGCGCAGCTCGTCGCAGAACTTCAGACGCTTGAGCGAACCATCGTCGCACATCACAAACTGAATGCGCTGCTGCCAGTCGAGCTCCAg > 1:667683/1‑100 (MQ=255) cTTCGTTTTGATCGCGCAGCCCGTCGCAGAACTTCAGACGCTTGAGCGAACCATCGTCGCACATCACAAACTGAATGCGCTGCTGCCAGTCGAGCTCCAg > 2:959246/1‑100 (MQ=255) gTTTTGATCGCGCAGCTCGTCGCAGAACTTCAGACGCTTGAGCGAACCATCGTCGCACATCACAAACTGAATGCGCTGCTGCCAGTCGAGCTCCAGTTTa > 1:1317954/1‑100 (MQ=255) gTTTTGATCGCGCAGCTCGTCGCAGAACTTCAGACGCTTGAGCGAACCATCGTCGCACATCACAAACTGAATGCGCTGCTGCCACTCGAGCGCCAGTTTa > 1:1549431/1‑100 (MQ=255) ttttGATCGCGCAGCTCGTCGCAGAACTTCAGACGCTTGAGCGAACCATCGTCGCACATCACAAACTGAATGCGCTGCTGCCAGTCGAGCTCCAGTTTAg < 2:293844/100‑1 (MQ=255) ttttGATCGCGCAGCTCGTCGCAGAACTTCAGACGCTTGAGCGAACCATCGTCGCACATCACAAACTGAATGCGCTGCTGCCAGTCGAGCTCCAGTTTAg < 2:628099/100‑1 (MQ=255) tGATCGCGCAGCTCGTCGCAGAACTTCAGACGCTTGAGCGAACCATCGTCGCACATCACAAACTGAATGCGCTGCTGCCAGTCGAGCTCCAGTTTAGTca > 1:1425215/1‑100 (MQ=255) taaactggaGCTCGTCGCAGAACTTCAGACGCTTGAGCGAACCATCGTCGCACATCACAAACTGAATGCGCTGCTGCCAGTCGAGCTCCAGTTTAGTcac < 1:1433975/92‑1 (MQ=255) gATCGCGCAGCTCGTCGCAGAACTTCAGACGCTTGAGCGAACCATCGTCGCACATCACAAACTGAATGCGCTGCTGCCAGTCGAGCTCCAGTTTAGTcac > 1:484146/1‑100 (MQ=255) aTCGCGCAGCTCGTCGCAGAACTTCAGACGCTTGAGCGAACCATCGTCGCACATCACAAACTGAATGCGCTGCTGCCAGTCGAGCTCCAGTTTAGTcacc < 1:487418/100‑1 (MQ=255) aTCGCGCAGCTCGTCGCAGAACTTCAGACGCTTGAGCGAACCATCGTCGCACATCACAAACTGAATGCGCTGCTGCCAGTCGAGCTCCAGTTTAGTcacc < 1:348279/100‑1 (MQ=255) cgcAGCTCGTCGCAGAACTTCAGACGCTTGAGCGAACCATCGTCGCACATCACAAACTGAATGCGCTGCTGCCAGTCGAGCTCCAGTTTAGTCACCACtt > 2:1498875/1‑100 (MQ=255) cgtcgCAGAACTTCAGACGCTTGAGCGAACCATCGTCGCACATCACAAACTGAATGCGCTGCTGCCAGTCGAGCTCCAGTTTAGTCACCACTTTTCCGGc < 1:207810/100‑1 (MQ=255) gtcgCAGAACTTCAGACGCTTGAGCGAACCATCGTCGCACATCACAAACTGAATGCGCTGCTGCCAGTCGAGCTCCAGTTTAGTAACCACTTTTCCgg > 2:1416646/1‑98 (MQ=255) cgCAGAACTTCAGACGCTTGAGCGAACCATCGTCGCACATCACAAACTGAATGCGCTGCTGCCAGTCGAGCTCCAGTTTAGTCACCACTTTTCCGGCTTc > 2:1564959/1‑100 (MQ=255) cgCAGAACTTCAGACGCTTGAGCGAACCATCGCCGCACATCGCGACCTGAATCCGCTGCTGTCAGTCGAGCTCCAGTTTAGTCACCACTTTTCCGGCTTc < 1:500465/100‑1 (MQ=255) gCAGAACTTCAGACGCTTGAGCGAACCATCGTCGCACATCACAAACTGAATGCGCTGCTGCCAGTCGAGCTCCAGTTTAGTCACCACTTTTCCGGCTTCa > 1:482857/1‑100 (MQ=255) gCAGAACTTCAGACGCTTGAGCGAACCATCGTCGCACATCACAAACTGAATGCGCTGCTGCCAGTCGAGCTCCAGTTTAGTCACCACTTTTCCGGCTTCa > 1:482947/1‑100 (MQ=255) gCAGAACTTCAGACGCTTGAGCGAACCATCGTCGCACATCACAAACTGAATGCGCTGCTGCCAGTCGAGCTCCAGTTTAGTCACCACTTTTCCGGCTTCa < 1:1511379/100‑1 (MQ=255) cAGAACTTCAGACGCTTGAGCGAACCATCGTCGCACATCACAAACTGAATGCGCTGCTGCCAGTCGAGCTCCAGTTTAGTCACCACTTTTCCGGCTTCaa > 2:295495/1‑100 (MQ=255) aGAACTTCAGACGCTTGAGCGAACCATCGTCGCACATCACAAACTGAATGCGCTGCTTCCAGTCGAGCTCCAGTTTAGTCACCACTTTTCCGGCTTCAAt < 2:867899/100‑1 (MQ=255) gAACTTCAGACGCTTGAGCGAACCATCGTCGCACATCACAAACTGAATGCGCTGCTGCCAGTCGAGCTCCAGTTTAGTCACCACTTTTCCGGCTTCAAtg < 1:606855/100‑1 (MQ=255) gAACTTCAGACGCTTGAGCGAACCATCGTCGCACATCACAAACTGAATGCGCTGCTGCCAGTCGAGCTCCAGTTTAGTCACCACTTTTCCGGCTTCAAtg > 2:820186/1‑100 (MQ=255) tCAGACGCTTGAGCGAACCATCGTCGCACATCACAAACTGAATGCGCTGCTGCCAGTCGAGCTCCAGTTTAGTCACCACTTTTCCGGCTTCAATGTGAtt < 2:1425205/100‑1 (MQ=255) tCAGACGCTTGAGCGAACCATCGTCGCACATCACAAACTGAATGCGCTGCTGCCAGTCGAGCTCCAGTTTAGTCACCACTTTTCCGGCTTCAATGTGAtt > 2:231999/1‑100 (MQ=255) aGCGAACCATCGTCGCACATCACAAACTGAATGCGCTGCTGCTAGTCGAGCTCCAGTTTAGTCACCACTTTTCCGGCTTCAATGTGATTGGTGATCTCtt < 2:197415/100‑1 (MQ=255) aGCGAACCATCGTCGCACATCACAAACTGAATGCGCTGCTGCCAGTCGAGCTCCAGTTTAGTCACCACTTTTCCGGCTTCAATGTGATTGGTGATCTCtt > 2:256695/1‑100 (MQ=255) gCGAACCATCGTCGCACATCACAAACTGAATGCGCTGCTGCCAGTCGAGCTCCAGTTTAGTCACCACTTTTCCGGCTTCAATGTGATTGGTGATCTCTTc > 1:1470002/1‑100 (MQ=255) aaCCATCGTCGCACATCACAAACTGAATGCGCTGCTGCCAGTCGAGCTCCAGTTTAGTCACCACTTTTCCGGCTTCAATGTGATTGGTGATCTCTTCGCt < 2:484143/100‑1 (MQ=255) tcgCACATCACAAACTGAATGCGCTGCTGCCAGTCGAGCTCCAGTTTAGTCACCACTTTTCCGGCTTCAATGTGATTGGTGATCTCTTCGCTGGTCAGAt < 1:689234/100‑1 (MQ=255) cgCACATCACAAACTGAATGCGCTGCTGCCAGTCGAGCTCCAGTTTAGTCACCACTTTTCCGGCTTCAATGTGATTGGTGATCTCTTCGCTGGTCAGATc < 2:531134/100‑1 (MQ=255) cacaTCACAAACTGAATGCGCTGCTGCCAGTCGAGCTCCAGTTTAGTCACCACTTTTCCGGCTTCAATGTGATTGGTGATCTCTTCGCTGGTCAGATCtt < 1:632123/100‑1 (MQ=255) aTCACAAACTGAATGCGCTGCTGCCAGTCGAGCTCCAGTTTAGTCACCACTTTTCCGGCTTCAATGTGATTGGTGATCTCTTCGCTGGTCAGATCTTGtt > 2:524636/1‑100 (MQ=255) aTCACAAACTGAATGCGCTGCTGCCAGTCGAGCTCCAGTTTAGTCACCACTTTTCCGGCTTCAATGTGATTGGTGATCTCTTCGCTGGTCAGATCTTGtt < 1:671610/100‑1 (MQ=255) aTCACAAACTGAATGCGCTGCTGCCAGTCGAGCTCCAGTTTAGTCACCACTTTTCCGGCTTCAATGTGATTGGTGATCTCTTCGCTGGTCAGATCTTGtt > 2:72539/1‑100 (MQ=255) aTCACAAACTGAATGCGCTGCTGCCAGTCGAGCTCCAGTTTAGTCACCACTTTTCCGGCTTCAATGTGATTGGTGATCTCTTCGCTGGTCAGATCTTGtt > 1:1138025/1‑100 (MQ=255) cacaAACTGAATGCGCTGCTGCCAGTCGAGCTCCAGTTTAGTCACCACTTTTCCGGCTTCAATGTGATTGGTGATCTCTTCGCTGGTCAGATCTTGTttc < 1:621634/100‑1 (MQ=255) cacaAACTGAATGCGCTGCTGCCAGTCGAGCTCCAGTTTAGTCACCACTTTTCCGGCTTCAATGTGATTGGTGATCTCTTCGCTGGTCAGATCTTGTttc > 1:427616/1‑100 (MQ=255) acaAACTGAATGCGCTGCTGCCAGTCGAGCTCCAGTTTAGTCACCACTTTTCCGGCTTCAATGTGATTGGTGATCTCTTCGCTGGTCAGATCTTGTttct > 1:1112955/1‑100 (MQ=255) aaaCTGAATGCGCTGCTGCCAGTCGAGCTCCAGTTTAGTCACCACTTTTCCGGCTTCAATGTGATTGGTGATCTCTTCGCTGGTCAGATCTTGTttcttc > 2:1149804/1‑100 (MQ=255) tGAATGCGCTGCTGCCAGTCGAGCTCCAGTTTAGTCACCACTTTTCCGGCTTCAATGTGATTGGTGATCTCTTCGCTGGTCAGATCTTGTTTCTTcgcgc < 2:152365/100‑1 (MQ=255) tGAATGCGCTGCTGCCAGTCGAGCTCCAGTTTAGTCACCACTTTTCCGGCTTCAATGTGATTGGTGATCTCTTCGCTGGTCAGATCTTGTTTCTTcgcgc > 2:689344/1‑100 (MQ=255) gctgctGCCCGTAGCGCTCCAGTTTAGTCACCACTTTTCCGGCTTCAATGTGATTGGTGATCTCTTCGCTGGTCAGATCTTGTTTCTTCGCGCAGATCAc < 1:232000/100‑1 (MQ=255) tgctgcCAGTCGAGCTCCAGTTTAGTCACCACTTTTCCGGCTTCAATGTGATTGGTGATCTCTTCGCTGGTCAGATCTTGTTTCTTCGCGCGGATCAcgc < 1:1316360/100‑1 (MQ=255) gctgcCAGTCGAGCTCCAGTTTAGTCACCACTTTTCCGGCTTCAATGTGATTGGTGATCTCTTCGCTGGTCAGATCTTGTTTCTTCGCGCGGATCAcgcc > 2:1432981/1‑100 (MQ=255) tgcCAGTCGAGCTCCAGTTTAGTCACCACTTTTCCGGCTTCAATGTGATTGGTGATCTCTTCGCTGGTCAGATCTTGTTTCTTCGCGCGGATCAcgcctc < 1:1058527/100‑3 (MQ=255) tgcCAGTCGAGCTCCAGTTTAGTCACCACTTTTCCGGCTTCAATGTGATTGGTGATCTCTTCGCTGGTCAGATCTTGTTTCTTCGCGCGGATCAcgcctc < 1:1058598/100‑3 (MQ=255) gTCGAGCTCCAGTTTAGTCACCACTTTTCCGGCTTCAATGTGATTGGTGATCTCTTCGCTGGTCAGATCTTGTTTCTTCGCGCGGATCACGCCGCCATCt > 1:1524206/1‑100 (MQ=255) aGCTCCAGTTTAGTCACCACTTTTCCGGCTTCAATGTGATTGGTGATCTCTTCGCTGGTCAGATCTTGTTTCTTCGCGCGGATCACGCCGCCATCTTCCa < 1:524639/100‑1 (MQ=255) | TCTTCGTTTTGATCGCGCAGCTCGTCGCAGAACTTCAGACGCTTGAGCGAACCATCGTCGCACATCACAAACTGAATGCGCTGCTGCCAGTCGAGCGCCAGTTTAGTCACCACTTTTCCGGCTTCAATGTGATTGGTGATCTCTTCGCTGGTCAGATCTTGTTTCTTCGCGCGGATCACGCCGCCATCTTCCA > NC_000913/409226‑409418 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |