| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 4,324,454 | G→T | Q238K (CAA→AAA) | phnC ← | phosphonate ABC transporter ATPase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 4,324,454 | 0 | G | T | 95.1% | 205.8 / 3.2 | 61 | Q238K (CAA→AAA) | phnC | phosphonate ABC transporter ATPase |
| Reads supporting (aligned to +/- strand): ref base G (2/1); new base T (32/26); total (34/27) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.14e-01 | |||||||||||
TCCTCAATGATGGGGATGTCAGGCAGCTTTCGCGTTCTCTTCGACGCGGTTAATGCTGCGGTAGAGATGGTCAAAACGTTCGTTATCAAACTGTTGGCTGCTGCCGTCGTAGAAGACGTGCCCCTGGCGCAGGGCGACGATGCGTTCGCAGTAGCGCAGGGCGTAATCCACCTGATGCAGCGTGACGACCAC > NC_000913/4324359‑4324550 | tCCTCAATGATGGGGATGTCAGGCAGCTTTCGCGTTCTCTTCGACGCGGTTAATGCTGCGGTAGAGATGGTCAAAACGTTCGTTATCAAACTGTTTgctg > 1:1040548/1‑100 (MQ=255) ccTCAATGATGGGGATGTCAGGCAGCTTTCGCGTTCTCTTCGACGCGGTTAATGCTGCGGTAGAGATGGTCAAAACGTTCGTTATCAAACTGTTGgctgc > 1:868161/1‑100 (MQ=255) cTCAATGATGGGGATGTCAGGCAGCTTTCGCGTTCTCTTCGACGCGGTTAATGCTGCGGTAGAGATGGTCAACACGTTCGTTATCAAGCTGTTTgctgct > 2:1299942/1‑100 (MQ=255) cTCAATGATGGGGATGTCAGGCAGCTTTCGCGTTCTCTTCGACGCGGTTAATGCTGCGGTAGAGATGGTCAAAACGTTCGTTATCAAACTGTTTgctgct > 1:1225709/1‑100 (MQ=255) cTCAATGATGGGGATGTCAGGCAGCTTTCGCGTTCTCTTCGACGCGGTTAATGCTGCGGTAGAGATGGTCAAAACGTTCGTTATCAAACTGTTTgctgct > 2:104040/1‑100 (MQ=255) cAATGATGGGGATGTCAGGCAGCTTTCGCGTTCTCTTCGACGCGGTTAATGCTGCGGTAGAGATGGTCAAAACGTTCGTTATCAAACTGTTtgctgctgc > 2:847768/1‑100 (MQ=255) cAATGATGGGGATGTCAGGCAGCTTTCGCGTTCTCTTCGACGCGGTTAATGCTGCGGTAGAGATGGTCAAAACGTTCGTTATCAAACTGTTtgctgctgc > 2:847753/1‑100 (MQ=255) cAATGATGGGGATGTCAGGCAGCTTTCGCGTTCTCTTCGACGCGGTTAATGCTGCGGTAGAGATGGTCAAAACGTTCGTTATCAAACTGTTtgctgctgc < 1:858791/100‑1 (MQ=255) tgatgGTGATGTCAGGCAGATTTCGCGTTCTCTTCTACGCGGGTGATGCTGCGGTAGAGATGGGCAAAACGTTTGTTATCAATCTGTTTgctgctn < 2:677842/96‑2 (MQ=255) atgGGGATGTCAGGCAGCTTTCGCGTTCTCTTCGTCGCGGTTAATGCTGCGGTAGAGATGGTCAAAACGTTCGTTATCAAACTGTTTGCTGCTGCcgtcg < 2:1397126/100‑1 (MQ=255) tgGGGATGTCAGGCAGCTTTCGCGTTCTCTTCGACGCGGTTAATGCTGCGGTAGAGATGGTCAAAACGTTCGTTATCAAACTGTTTGCTGCTGCcgtcgt < 1:847774/100‑1 (MQ=255) tgGGGATGTCAGGCAGCTTTCGCGTTCTCTTCGACGCGGTTAATGCTGCGGTAGAGATGGTCAAAACGTTCGTTATCAAACTGTTTGCTGCTGCcgtcgt < 1:847759/100‑1 (MQ=255) tgGGGATGTCAGGCAGCTTTCGCGTTCTCTTCGACGCGGTTAATGCTGCGGTAGAGATGGTCAAAACGTTCGTTATCAAACTGTTTGCTGCTGCcgtcgt < 2:1429768/100‑1 (MQ=255) ggggATGTCAGGCAGCTTTCGCGTTCTCTTCGACGCGGTTAATGCTGCGGTAGAGATGGTCAAAACGTTCGTTATCAAACTGTTTGCTGCTGCCGTCGTa > 2:902141/1‑100 (MQ=255) ggggATGTCAGGCAGCTTTCGCGTTCTCTTCGACGCGGTTAATGCTGCGGTAGAGATGGTCAAAACGTTCGTTATCAAACTGTTTGCTGCTGCCGTCGTa > 1:1552129/1‑100 (MQ=255) gggATGTCAGGCAGCTTTCGCGTTCTCTTCGACGCGGTTAATGCTGCGGTAGAGATGGTCAAAACGTTCGTTATCAAACTGTTTGCTGCTGCCGTCGTag < 2:27095/100‑1 (MQ=255) gggATGTCAGGCAGCTTTCGCGTTCTCTTCGACGCGGTTAATGCTGCGGTAGAGATGGTCAAAACGTTCGTTATCAAACTGTTTGCTGCTGCCGTCGTag > 2:509435/1‑100 (MQ=255) ggATGTCAGGCAGCTTTCGCGTTCTCTTCGACGCGGTTAATGCTGCGGTAGAGATGGTCAAAACGTTCGTTATCAAACTGTTTGCTGCTGCCGTCGTaga < 2:362775/100‑1 (MQ=255) aGGCAGCTTTCGCGTTCTCTTCGACGCGGTTAATGCTGCGGTAGAGATGGTCAAAACGTTCGTTATCAAACTGTTTGCTGCTGCCGTCGTAGAAGACGTg > 1:198459/1‑100 (MQ=255) aGGCAGCTTTCGCGTTCTCTTCGACGCGGTTAATGCTGCGGTAGAGATGGTCAAAACGTTCGTTATCAAACTGTTTGCTGCTGCCGTCGTAGAAGACGTg > 1:366424/1‑100 (MQ=255) ggCAGCTTTCGCGTTCTCTTCGACGCGGTTAATGCTGCGGTAGAGATGGTCAAAACGTTCGTTATCAAACTGTTTGCTGCTGCCGTCGTAGAAGACGTGc > 1:1323273/1‑100 (MQ=255) ttCGCGTTCTCTTCGACGCGGTTAATGCTGCGGTAGAGATGGTCAAAACGTTCGTTATCAAACTGTTTGCTGCTGCCGTCGTAGAAGACGTGCCCCTGgc < 1:1522286/100‑1 (MQ=255) ttCGCGTTCTCTTCGACGCGGTTAATGCTGCGGTAGAGATGGTCAAAACGTTCGTTATCAAACTGTTTGCTGCTGCCGTCGTAGAAGACGTGCCCCTGgc > 1:1377131/1‑100 (MQ=255) gcgTTCTCTTCGACGCGGTTAATGCTGCGGTAGAGATGGTCAAAACGTTCGTTATCAAACTGTTTGCTGCTGCCGTCGTAGAAGACGTGCCCCTGGCGCa > 1:47107/1‑100 (MQ=255) gcgTTCTCTTCGACGCGGTTAATGCTGCGGTAGAGATGGTCAAAACGTTCGTTATCAAACTGTTTGCTGCTGCCGTCGTAGAAGACGTGCCCCTGGCGCa < 1:1403750/100‑1 (MQ=255) gcgTTCTCTTCGACGCGGTTAATGCTGCGGTAGAGATGGTCAAAACGTTCGTTATCAAACTGTTTGCTGCTGCCGTCGTAGAAGACGTGCCCCTGGCGCa > 1:69723/1‑100 (MQ=255) tgctctTCGACGCGGTTAATGCTGCGGTAGAGATGGTCAAAACGTTCGTTATCAAACTGTTTGCTGCTGCCGTCGTAGAGGACGTGCCCCTGGCGCAggg < 2:956959/98‑1 (MQ=255) ctctTCGACGCGGTTAATGCTGCGGTAGAGATGGTCAAAACGTTCGTTATCAAACTGTTTGCTGCTGCCGTCGTAGAAGACGTGCCCCTGGCGCAGGGcg < 2:398986/100‑1 (MQ=255) ctctTCGACGCGGTTAATGCTGCGGTAGAGATGGTCAAAACGTTCGTTATCAAACTGTTTGCTGCTGCCGTCGTAGAAGACGTGCCCCTGGCGCAGGGcg < 1:1560543/100‑1 (MQ=255) ctctTCGACGCGGTTAATGCTGCGGTAGAGATGGTCAAAACGTTCGTTATCAAACTGTTGGCTGCTGCCGTCGTAGAAGACGTGCCCCTGGCGCAGGGcg > 1:864386/1‑100 (MQ=255) ctTCGACGCGGTTAATGCTGCGGTAGAGATGGTCAAAACGTTCGTTATCAAACTGTTTGCTGCTGCCGTCGTAGAAGACGTGCCCCTGGCGCAGGGcgac > 1:735558/1‑100 (MQ=255) ttCGACGCGGTTAATGCTGCGGTAGAGATGGTCAAAACGTTCGTTATCAAACTGTTTGCTGCTGCCGTCGTAGAAGACGTGCCCCTGGCGCAGGGcgacg < 2:431551/100‑1 (MQ=255) cGACGCGGTTAATGCTGCGGTAGAGATGGTCAAAACGTTCGTTATCAAACTGTTTGCTGCTGCCGTCGTAGAAGACGTGCCCCTGGCGCAGGGCGACGAt > 2:1243497/1‑100 (MQ=255) cGACGCGGTTAATGCTGCGGTAGAGATGGTCAAAACGTTCGTTATCAAACTGTTTGCTGCTGCCGTCGTAGAAGACGTGCCCCTGGCGCAGGGCGACGAt > 2:367898/1‑100 (MQ=255) cgcgGTTAATGCTGCGGTAGAGATGGTCAAAACGTTCGTTATCAAACTGTTTGCTGCTGCCGTCGTAGAAGACGTGCCCCTGGCGCAGGGCGACGATGCg > 2:1407320/1‑100 (MQ=255) cgcgGTTAATGCTGCGGTAGAGATGGTCAAAACGTTCGTTATCAAACTGTTTGCTGCTGCCGTCGTAGAAGACGTGCCCCTGGCGCAGGGCGACGATGCg < 2:430989/100‑1 (MQ=255) cgGTTAATGCTGCGGTAGAGATGGTCAAAACGTTCGTTATCAAACTGTTTGCTGCTGCCGTCGTAGAAGACGTGCCCCTGGCGCAGGGCGACGATGCGtt > 1:1254959/1‑100 (MQ=255) cgGTTAATGCTGCGGTAGAGATGGTCAAAACGTTCGTTATCAAACTGTTTGCTGCTGCCGTCGTAGAAGACGTGCCCCTGGCGCAGGGCGACGATGCGtt < 2:739053/100‑1 (MQ=255) cgGTTAATGCTGCGGTAGAGATGGTCAAAACGTTCGTTATCAAACTGTTTGCTGCTGCCGTCGTAGAAGACGTGCCCCTGGCGCAGGGCGACGATGCGtt < 1:1510255/100‑1 (MQ=255) ttAATGCTGCGGTAGAGATGGTCAAAACGTTCGTTATCAAACTGTTTGCTGCTGCCGTCGTAGAAGACGTGCCCCTGGCGCAGGGCGACGATGCGTTCGc < 1:382558/100‑1 (MQ=255) aaTGCTGCGGTAGAGATGGTCAAAACGTTCGTTATCAAACTGTTGGCTGCTGCCGTCGTAGAAGACGTGCCCCTGGCGCAGGGCGACGATGCGTTCGCAg < 2:864379/100‑1 (MQ=255) aTGCTGCGGTAGAGATGGTCAAAACGTTCGTTATCAAACTGTTTGCTGCTGCCGTCGTAGAAGACGTGCCCCTGGCGCAGGGCGACGATGCGTTCGCAGt > 2:580870/1‑100 (MQ=255) tgctgcGGTAGAGATGGTCAAAACGTTCGTTATCAAACTGTTTGCTGCTGCCGTCGTAGAAGACGTGCCCCTGGCGCAGGGCGACGATGCGTTCGCAGTa < 1:1243505/100‑1 (MQ=255) gctgcGGTAGAGATGGTCAAAACGTTCGTTATCAAACTGTTTGCTGCTGCCGTCGTAGAAGACGTGCCCCTGGCGCAGGGCGACGATGCGTTCGCAGTAg > 2:1507052/1‑100 (MQ=255) tgcGGTAGAGATGGTCAAAACGTTCGTTATCAAACTGTTTGCTGCTGCCGTCGTAGAAGACGTGCCCCTGGCGCAGGGCGACGATGCGTTCGCAGTAgcg < 1:620613/100‑1 (MQ=255) gTAGAGATGGTCAAAACGTTCGTTATCAAACTGTTTGCTGCTGCCGTCGTAGAAGACGTGCCCCTGGCGCAGGGCGACGATGCGTTCGCAGTAGCGCAgg > 1:1001068/1‑100 (MQ=255) gTAGAGATGGTCAAAACGTTCGTTATCAAACTGTTTGCTGCTGCCGTCGTAGAAGACGTGCCCCTGGCGCAGGGCGACGATGCGTTCGCAGTAGCGCAgg > 1:999987/1‑100 (MQ=255) gaTGGTCAAAACGTTCGTTATCAGACTGTTTGCTGCTGCCGTCGTAGAAGACGTGCCCCTGGCGCAGGGCGACGATGCGTTCGCAGTAGCGCAGGGCGTa > 2:238021/1‑100 (MQ=255) tGGTCAAAACGTTCGTTATCAAACTGTTTGCTGCTGCCGTCGTAGAAGACGTGCCCCTGGCGCAGGGCGACGATGCGTTCGCAGTAGCGCAGGGCGTAAt > 2:298086/1‑100 (MQ=255) gTCAAAACGTTCGTTATCAAACTGTTTGCTGCTGCCGTCGTAGAAGACGTGCCCCTGGCGCAGGGCGACGATGCGTTCGCAGTAGCGCAGGGCGTAATcc < 1:1560741/100‑1 (MQ=255) tCAAAACGTTCGTTATCAAACTGTTTGCTGCTGCCGTCGTAGAAGACGTGCCCCTGGCGCAGGGCGACGATGCGTTCGCAGTAGCGCAGGGCGTAATCCa > 2:914766/1‑100 (MQ=255) cAAAACGTTCGTTATCAAACTGTTTGCTGCTGCCGTCGTAGAAGACGTGCCCCTGGCGCAGGGCGACGATGCGTTCGCAGTAGCGCAGGGCGTAATCCAc > 2:555496/1‑100 (MQ=255) aaaCGTTCGTTATCAAACTGTTTGCTGCTGCCGTCGTAGAAGACGTGCCCCTGGCGCAGGGCGACGATGCGTTCGCAGTAGCGCAGGGCGTAATCCACCt > 2:429459/1‑100 (MQ=255) aaaCGTTCGTTATCAAACTGTTTGCTGCTGCCGTCGTAGAAGACGTGCCCCTGGCGCAGGGCGACGATGCGTTCGCAGTAGCGCAGGGCGTAATCCACCt < 1:154637/100‑1 (MQ=255) aaCGTTCGTTATCAAACTGTTTGCTGCTGCCGTCGTAGAAGACGTGCCCCTGGCGCAGGGCGACGATGCGTTCGCAGTAGCGCAGGGCGTAATCCACCTg > 1:603291/1‑100 (MQ=255) gttcgttATCAAACTGTTTGCTGCTGCCGTCGTAGAAGACGTGCCCCTGGCGCAGGGCGACGATGCGTTCGCAGTAGCGCAGGGCGTAATCCACCTGATg > 2:380798/1‑100 (MQ=255) ttcgttATCAAACTGTTTGCTGCTGCCGTCGTAGAAGACGTGCCCCTGGCGCAGGGCGACGATGCGTTCGCAGTAGCGCAGGGCGTAATCCACCTGATGc < 2:69723/100‑1 (MQ=255) tcgttATCAAACTGTTTGCTGCTGCCGTCGTAGAAGACGTGCCCCTGGCGCAGGGCGACGATGCGTTCGCAGTAGCGCAGGGCGTAAGCCACCTGATGCa < 1:1032670/100‑1 (MQ=255) cgttATCAAACTGTTTGCTGCTGCCGTCGTAGAAGACGTGCCCCTGGCGCAGGGCGACGATGCGTTCGCAGTAGCGCAGGGCGTAATCCACCTGATGCAg < 1:642970/100‑1 (MQ=255) cgttATCAAACTGTTTGCTGCTGCCGTCGTAGAAGACGTGCCCCTGGCGCAGGGCGACGATGCGTTCGCAGTAGCGCAGGGCGTAATCCACCTGATGCAg < 1:643010/100‑1 (MQ=255) tATCAAACTGTTTGCTGCTGCCGTCGTAGAAGACGTGCCCCTGGCGCAGGGCGACGATGCGTTCGCAGTAGCGCAGGGCGTAATCCACCTGATGCAGCGt > 2:1176301/1‑100 (MQ=255) tATCAAACTGTTTGCTGCTGCCGTCGTAGAAGACGTGCCCCTGGCGCAGGGCGACGATGCGTTCGCAGTAGCGCAGGGCGTAATCCACCTGATGCAGCGt < 2:429918/100‑1 (MQ=255) cAAACTGTTTGCTGCTGCCGTCGTAGAAGACGTGCCCCTGGCGCAGGGCGACGATGCGTTCGCAGTAGCGCAGGGCGTAATCCACCTGATGCAGCGTgac > 2:734268/1‑100 (MQ=255) gTTTGCTGCTGCCGTCGTAGAAGACGTGCCCCTGGCGCAGGGCGACGATGCGTTCGCAGTAGCGCAGGGCGTAATCCACCTGATGCAGCGTGACGACCAc > 2:1021746/1‑100 (MQ=255) | TCCTCAATGATGGGGATGTCAGGCAGCTTTCGCGTTCTCTTCGACGCGGTTAATGCTGCGGTAGAGATGGTCAAAACGTTCGTTATCAAACTGTTGGCTGCTGCCGTCGTAGAAGACGTGCCCCTGGCGCAGGGCGACGATGCGTTCGCAGTAGCGCAGGGCGTAATCCACCTGATGCAGCGTGACGACCAC > NC_000913/4324359‑4324550 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |