Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,434,437 T→A W109R (TGG→AGG)  ytfH → DUF24 family HxlR‑type putative transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,434,4370TA96.5% 193.3 / ‑1.4 58W109R (TGG→AGG) ytfHDUF24 family HxlR‑type putative transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base T (1/1);  new base A (24/31);  total (25/33)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.55e-01

ACCGTATCGCGTATCCGGTGGTGCCGCCGCATGTGGAATATAGCCTCACGCCGCTGGGCGAGCAGGTGAGCGAAAAGGTTGCTGCACTGGCGGACTGGATTGAGTTGAATTTGCCCGAGGTGTTGGCGGTGCGGGATGAACGTGCGGCATAACTGCCGGATGCGCTGAGCTTATCTGGTCTACGGTGCAT  >  NC_000913/4434342‑4434531
                                                                                               |                                                                                              
aCCGTATCGCGTATCCGGTGGTGCCGCCGCATGTGGAATATAGCCTCACGCCGCTGGGCGAGCAGGTGAGCGAAAAGGTTGCTGCACTGGCGGACAGGAt                                                                                            <  1:166079/100‑1 (MQ=255)
 ccGTATCGCGTATCCGGTGGTGCCGCCGCATGTGGAATATAGCCTCACGCCGCTGGGCGAGCAGGTGAGCGAAAAGGTTGCTGCACTGGCGGACAGGAtt                                                                                           >  2:436760/1‑100 (MQ=255)
    tATCGCGTATCCGGTGGTGCCGCCGCATGTGGAATATAGCCTCACGCCGCTGGGCGAGCAGGTGAGCGAAAAGGTTGCTGCACTGGCGGACAGGATTGAg                                                                                        <  1:1030636/100‑1 (MQ=255)
       cgcgTATCCGGTGGTGCCGCCGCATGTGGAATATAGCCTCACGCCGCTGGGCGAGCAGGTGAGCGAAAAGGTTGCTGCACTGGCGGACAGGATTGAGTTg                                                                                     <  1:913964/100‑1 (MQ=255)
       cgcgTATCCGGTGGTGCCGCCGCATGTGGAATATAGCCTCACGCCGCTGGGCGAGCAGGTGAGCGAAAAGGTTGCTGCACTGGCGGACAGGATTGAGTTg                                                                                     <  2:1433536/100‑1 (MQ=255)
        gcgTATCCGGTGGTGCCGCCGCATGTGGAATATAGCCTCACGCCGCTGGGCGAGCAGGTGAGCGAAAAGGTTGCTGCACTGGCGGACAGGATTGAGTTGa                                                                                    <  2:876586/100‑1 (MQ=255)
        gcgTATCCGGTGGTGCCGCCGCATGTGGAATATAGCCTCACGCCGCTGGGCGAGCAGGTGAGCGAAAAGGTTGCTGCACTGGCGGACAGGATTGAGTTGa                                                                                    >  1:218376/1‑100 (MQ=255)
        gcgTATCCGGTGGTGCCGCCGCATGTGGAATATAGCCTCACGCCGCTGGGCGAGCAGGTGAGCGAAAAGGTTGCTGCACTGGCGGACAGGATTGAGTTGa                                                                                    <  1:888601/100‑1 (MQ=255)
           tATCCGGTGGTGCCGCCGCATGTGGAATATAGCCTCACGCCGCTGGGCGAGCAGGTGAGCGAAAAGGTTGCTGCACTGGCGGACAGGATTGAGTTGAAtt                                                                                 <  1:1337289/100‑1 (MQ=255)
           tATCCGGTGGTGCCGCCGCATGTGGAATATAGCCTCACGCCGCTGGGCGAGCAGGTGAGCGAAAAGGTTGCTGCACTGGCGGACAGGATTGAGTTGAAtt                                                                                 <  1:923871/100‑1 (MQ=255)
             tCCGGTGGTGCCGCCGCATGTGGAATATAGCCTCACGCCGCTGGGCGAGCAGGTGAGCGAAAAGGTTGCTGCACTGGCGGACAGGATTGAGTTGAATTTg                                                                               <  1:370949/100‑1 (MQ=255)
                ggtggtGCCGCCGCATGTGGAATATAGCCTCACGCCGCTGGGCGAGCAGGTGAGCGAAAAGGTTGCTGCACTGGCGGACAGGATTGAGTTGAATTTGccc                                                                            <  2:934454/100‑1 (MQ=255)
                 gtggtgCCGCCGCATGTGGAATATAGCCTCACGCCGCTGGGCGAGCAGGTGAGCGAAAAGGTTGCTGCACTGGCGGACAGGATTGAGTTGAATTTGCCCg                                                                           <  2:298757/100‑1 (MQ=255)
                 gtggtgCCGCCGCATGTGGAATATAGCCTCACGCCGCTGGGCGAGCAGGTGAGCGAAAAGGTTGCTGCACTGGCGGACAGGATTGAGTTGAATTTGCCCg                                                                           <  2:1434045/100‑1 (MQ=255)
                     tgCCGCCGCATGTGGAATATAGCCTCACGCCGCTGGGCGAGCAGGTGAGCGAAAAGGTTGCTGCACTGGCGGACAGGATTGAGTTGAATTTGCCCGAGgt                                                                       >  2:9036/1‑100 (MQ=255)
                     tgCCGCCGCATGTGGAATATAGCCTCACGCCGCTGGGCGAGCAGGTGAGCGAAAAGGTTGCTGCACTGGCGGACAGGATTGAGTTGAATTTGCCCGAGgt                                                                       >  1:491347/1‑100 (MQ=255)
                      gccgccGCATGTGGAATATAGCCTCACGCCGCTGGGCGAGCAGGTGAGCGAAAAGGTTGCTGCACTGGCGGACAGGATTGAGTTGAATTTGCCCGAGgtg                                                                      >  1:744292/1‑100 (MQ=255)
                        cgccgcATGTGGAATATAGCCTCACGCCGCTGGGCGAGCAGGTGAGCGAAAAGGTTGCTGCACTGGCGGACAGGATTGAGTTGAATTTGCCCGAGgtgt                                                                     <  1:260345/99‑1 (MQ=255)
                         gccgcATGTGGAATATAGCCTCACGCCGCTGGGCGAGCAGGTGAGCGAAAAGGTTGCTGCACTGGCGGACAGGATTGAGTTGAATTTGCCCGAGGTGTTg                                                                   >  2:875599/1‑100 (MQ=255)
                           cgcATGTGGAATATAGCCTCACGCCGCTGGGCGAGCAGGTGAGCGAAAAGGTTGCTGCACTGGCGGACAGGATTGAGTTGAATTTGCCCGAGGTGTTGGc                                                                 <  1:700442/100‑1 (MQ=255)
                              aTGTGGAATATAGCCTCACGCCGCTGGGCGAGCAGGTGAGCGAAAAGGTTGCTGCACTGGCGGACAGGATTGAGTTGAATTTGCCCGAGGTGTTGGCGGt                                                              >  1:48075/1‑100 (MQ=255)
                               tgtgGAATATAGCCTCACGCCGCTGGGCGAGCAGGTGAGCGAAAAGGTTGCTGCACGGGCGGACAGGATTGAGTTGAATTTGCCCGAGGTGTTGGCGGTg                                                             >  2:1005661/1‑100 (MQ=255)
                                gtgGAATATAGCCTCACGCCGCTGGGCGAGCAGGTGAGCGAAAAGGTTGCTGCACTGGCGGACAGGATTGAGTTGAATTTGCCCGAGGTGTTGGCGGTg                                                             >  2:765974/1‑99 (MQ=255)
                                 tgGAATATAGCCTCACGCCGCTGGGCGAGCAGGTGAGCGAAAAGGTTGCTGCACTGGCGGACAGGATTGAGTTGAATTTGCCCGAGGTGTTGGCGGTGCg                                                           <  1:291816/100‑1 (MQ=255)
                                    aaTATAGCCTCACGCCGCTGGGCGAGCAGGTGAGCGAAAAGGTTGCTGCACTGGCGGACAGGATTGAGTTGAATTTGCCCGAGGTGTTGGCGGTGCGGGa                                                        <  1:162581/100‑1 (MQ=255)
                                     atatAGCCTCACGCCGCTGGGCGAGCAGGTGAGCGAAAAGGTTGCTGCACTGGCGGACAGGATTGAGTTGAATTTGCCCGAGGTGTTGGCGGTGCGGGAt                                                       >  2:433888/1‑100 (MQ=255)
                                      tataGCCTCACGCCGCTGGGCGAGCAGGTGAGCGAAAAGGTTGCTGCACTGGCGGACAGGATTGAGTTGAATTTGCCCGAGGTGTTGGCGGTGCGGGATg                                                      <  2:450052/100‑1 (MQ=255)
                                      tataGCCTCACGCCGCTGGGCGAGCAGGTGAGCGAAAAGGTTGCTGCACTGGCGGACAGGATTGAGTTGAATTTGCCCGAGGTGTTGGCGGTGCGGGATg                                                      <  2:1236394/100‑1 (MQ=255)
                                       ataGCCTCACGCCGCTGGGCGAGCAGGTGAGCGAAAAGGTTGCTGCACTGGCGGACAGGATTGAGTTGAATTTGCCCGAGGTGTTGGCGGTGCGGGATGa                                                     <  1:495677/100‑1 (MQ=255)
                                          gCCTCACGCCGCTGGGCGAGCAGGTGAGCGAACAGGTTGCTGCACTGGCGGACAGGATTGAGTTGAATTTGCCCGAGGTGTTGGCGGTGCGGGATGAACg                                                  >  1:590856/1‑100 (MQ=255)
                                          gCCTCACGCCGCTGGGCGAGCAGGTGAGCGAAAAGGTTGCTGCACTGGCGGACAGGATTGAGTTGAATTTGCCCGAGGTGTTGGCGGTGCGGGATGAACg                                                  >  1:543707/1‑100 (MQ=255)
                                          gCCTCACGCCGCTGGGCGAGCAGGTGAGCGAAAAGGTTGCTGCACTGGCGGACAGGATTGAGTTGAATTTGCCCGAGGTGTTGGCGGTGCGGGATGAACg                                                  <  2:529406/100‑1 (MQ=255)
                                             tCACGCCGCTGGGCGAGCAGGTGAGCGAAAAGGTTGCTGCACTGGCGGACAGGATTGAGTTGAATTTGCCCGAGGTGTTGGCGGTGCGGGATGAACGTGc                                               >  1:326476/1‑100 (MQ=255)
                                                cgccgcTGGGCGAGCAGGTGAGCGAAAAGGTTGCTGCACTGGCGGACTGGATTGAGTTGAATTTGCCCGAGGTGTTGGCGGTGCGGGATGAACGTGCGGc                                            >  1:448637/1‑100 (MQ=255)
                                                    gcggggCGAGCAGGTGAGCGAAAAGGTTGCTGCACTGGCGGACAGGAGTGAGTTGAATTTGCCCGAGGTGTTGGCGGTGCGGGATGAACGTGCGGCATaa                                        <  1:476712/97‑1 (MQ=255)
                                                    gcTGGGCGAGCAGGTGAGCGAAAAGGTTGCTGCACTGGCGGACAGGATTGAGTTGAATTTGCCCGAGGTGTTGGCGGTGCGGGATGAACGTGCGGCATaa                                        <  1:1238716/100‑1 (MQ=255)
                                                     cTGGGCGAGCAGGTGAGCGAAAAGGTTGCTGCACTGGCGGACAGGATTGAGTTGAATTTGCCCGAGGTGTTGGCGGTGCGGGATGAACGTGCGGCATAAc                                       >  2:589697/1‑100 (MQ=255)
                                                     cTGGGCGAGCAGGTGAGCGAAAAGGTTGCTGCACTGGCGGACAGGATTGAGTTGAATTTGCCCGAGGTGTTGGCGGTGCGGGATGAACGTGCGGCATAAc                                       <  1:436762/100‑1 (MQ=255)
                                                      tGGGCGAGCAGGTGAGCGAAAAGGTTGCTGCACTGGCGGACCTGATTGAGTTGAATTTGCCCGAGGTGTTGGCGGTGCGGGATGAACGTGCGGCATAACt                                      <  1:226177/100‑1 (MQ=255)
                                                      tGGGCGAGCAGGTGAGCGAAAAGGTTGCTGCACTGGCGGACAGGATTGAGTTGAATTTGCCCGAGGTGTTGGCGGTGCGGGATGAACGTGCGGCATAACt                                      <  1:898852/100‑1 (MQ=255)
                                                         gCGAGCAGGTGAGCGAAAAGGTTGCTGCACTGGCGGACAGGATTGAGTTGAATTTGCCCGAGGTGTTGGCGGTGCGGGATGAACGTGCGGCATAACTGcc                                   >  2:704333/1‑100 (MQ=255)
                                                         gCGAGCAGGTGAGCGAAAAGGTTGCTGCACTGGCGGACAGGATTGAGTAGAATTTGCCCGAGGTGTTGGCGGTGCGGGATGAACGTGCGGCATAACTGcc                                   >  2:551710/1‑100 (MQ=255)
                                                          cGAGCAGGTGAGCGAAAAGGTTGCTGCACTGGCGGACAGGATTGAGTTGAATTTGCCCGAGGTGTTGGCGGTGCGGGATGAACGTGCGGCATAACTGCCg                                  <  1:568624/100‑1 (MQ=255)
                                                                ggTGAGCGAAAAGGTTGCTGCACTGGCGGACAGGATTGAGTTGAATTTGCCCGAGGTGTTGGCGGTGCGGGATGAACGTGCGGCATAACTGCCGGATgcg                            >  2:217179/1‑100 (MQ=255)
                                                                  tGAGCGAAAAGGTTGCTGCACTGGCGGACAGGATTGAGTTGAATTTGCCCGAGGTGTTGGCGGTGCGGGATGAACGTGCGGCATAACTGCCGGATGCGCt                          >  1:69847/1‑100 (MQ=255)
                                                                  tGAGCGAAAAGGTTGCTGCACTGGCGGACAGGATTGAGTTGAATTTGCCCGAGGTGTTGGCGGTGCGGGATGAACGTGCGGCATAACTGCCGGATGCGCt                          >  1:1394092/1‑100 (MQ=255)
                                                                    aGCGAAAAGGTTGCTGCACTGGCGGACAGGATTGAGTTGAATTTGCCCGAGGTGTTGGCGGTGCGGGATGAACGTGCGGCATAACTGCCGGATGCGCTGa                        <  2:977438/100‑1 (MQ=255)
                                                                     gCGAAAAGGTTGCTGCACTGGCGGACAGGATTGAGTTGAATTTGCCCGAGGTGTTGGCGGTGCGGGATGAACGTGCGGCATAACTGCCGGATGCGCTGAg                       >  1:1355295/1‑100 (MQ=255)
                                                                      cGAAAAGGTTGCTGCACTGGCGGACAGGATTGAGTTGAATTTGCCCGAGGTGTTGGCGGTGCGGGATGAACGTGCGGCATAACTGCCGGATGCGCTGAGc                      <  2:48075/100‑1 (MQ=255)
                                                                      cGAAAAGGTTGCTGCACTGGCGGACAGGATTGAGTTGAATTTGCCCGAGGTGTTGGCGGTGCGGGATGAACGTGCGGCATAACTGCCGGATGCGCTGAGc                      <  1:457690/100‑1 (MQ=255)
                                                                         aaaGGTTGCTGCACTGGCGGACTGGATTGAGTTGAATTTGCCCGAGGTGTTGGCGGTGCGGGATGAACGTGCGGCATAACTGCCGGATGCGCTGAGCTTa                   <  2:204884/100‑1 (MQ=255)
                                                                           aGGTTGCTGCACTGGCGGACAGGATTGAGTTGAATTTGCCCGAGGTGTTGGCGGTGCGGGATGAACGTGCGGCATAACTGCCGGATGCGCTGAGCTTATc                 <  1:658455/100‑1 (MQ=255)
                                                                             gTTGCTGCACTGGCGGACAGGATTGAGTTGAATTTGCCCGAGGTGTTGGCGGTGCGGGATGAACGTGCGGCATAACTGCCGGATGCGCTGAGCTTATCTg               >  1:645759/1‑100 (MQ=255)
                                                                               tgctgcACTGGCGGACAGGATTGAGTTGAATTTGCCCGAGGTGTTGGCGGTGCGGGATGAACATGCGGCATAACTGCCGGATGCGCTGAGCTTATCTGGt             >  1:1241356/1‑100 (MQ=255)
                                                                                       tGGCGGACAGGATTGAGTTGAATTTGCCCGAGGTGTTGGCGGTGCGGGATGAACGTGCGGCATAACTGCCGGATGCGCTGAGCTTATCTGGTCTACGGTg     >  1:187588/1‑100 (MQ=255)
                                                                                       tGGCGGACAGGATTGAGTTGAATTTGCCCGAGGTGTTGGCGGTGCGGGATGAACGTGCGGCATAACTGCCGGATGCGCTGAGCTTATCTGGTCTACGGTg     <  1:1541493/100‑1 (MQ=255)
                                                                                       tGGCGGACAGGATTGAGTTGAATTTGCCCGAGGTGTTGGCGGTGCGGGATGAACGTGCGGCATAACTGCCGGATGCGCTGAGCTTATCTGGTCTACGGTg     <  2:876736/100‑1 (MQ=255)
                                                                                          cGGACAGGATTGAGTTGAATTTGCCCGAGGTGTTGGCGGTGCGGGATGAACGTGCGGCATAACTGCCGGATGCGCTGAGCTTATCTGGTCTACGGTGCAt  >  2:339748/1‑100 (MQ=255)
                                                                                               |                                                                                              
ACCGTATCGCGTATCCGGTGGTGCCGCCGCATGTGGAATATAGCCTCACGCCGCTGGGCGAGCAGGTGAGCGAAAAGGTTGCTGCACTGGCGGACTGGATTGAGTTGAATTTGCCCGAGGTGTTGGCGGTGCGGGATGAACGTGCGGCATAACTGCCGGATGCGCTGAGCTTATCTGGTCTACGGTGCAT  >  NC_000913/4434342‑4434531

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑