Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,560,045	C→A	W212C (TGG→TGT)	yjiH ←	nucleoside recognition pore and gate family putative inner membrane transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,560,045	0	C	A	100.0%	217.5 / NA	62	W212C (TGG→TGT)	yjiH	nucleoside recognition pore and gate family putative inner membrane transporter
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (30/32);  total (30/32)

TCGATAAACATATCCATGACGTTTTTGCGGACCTGAGTTGTCATGCTTGCGCTCCTTGCGTTGGGTTGCGACGTTCTTCGAAGTTAAGCCAGACGCGCAAAATGTTGGCTCCGACAAATTTAAACACCAGGATGACGGCTAAAGGTACGATTACGGACGTGCCCAGAAAGGCAAACACCGCGACG  >  NC_000913/4559957‑4560141                                                                                         |                                                                                                 tCGATAAACATATCCATGACGTTTTTGCGGACCTGAGTTGTCATGCTTGCGCTCCTTGCGTTGGGTTGCGACGTTCTTCGAAGTTAAGACAGACgcgcga                                                                                       >  2:723228/1‑98 (MQ=255)  cGATAAACATATCCATGACGTTTTTGCGGACCTGAGTTGTCATGCTTGCGCTCCTTGCGTTGGGTTGCGACGTTCTTCGAAGTTAAGACAGACGCGCaaa                                                                                      >  2:1442581/1‑100 (MQ=255)   gATAAACATATCCATGACGTTTTTGCGGACCTGAGTTGTCATGCTTGCGCTCCTTGCGTTGGGTTGCGACGTTCTTCGAAGTTAAGACAGACGCGCaaaa                                                                                     >  2:802534/1‑100 (MQ=255)   gATAAACATATCCATGACGTTTTTGCGGACCTGAGTTGTCATGCTTGCGCTCCTTGCGTTGGGTTGCGACGTTCTTCGAAGTTAAGACAGACGCGCaaaa                                                                                     >  2:415535/1‑100 (MQ=255)     tAAACATATCCATGACGTTTTTGCGGACCTGAGTTGTCATGCTTGCGCTCCTTGCGTTGGGTTGCGACGTTCTTCGAAGTTAAGACAGACGCGCAAAATg                                                                                   >  2:102089/1‑100 (MQ=255)       aaCATATCCATGACGTTTTTGCGGACCTGAGTTGTCATGCTTGCGCTCCTTGCGTTGGGTTGCGACGTTCTTCGAAGTTAAGACAGACGCGCAAAATGtt                                                                                 >  2:1260219/1‑100 (MQ=255)       aaCATATCCATGACGTTTTTGCGGACCTGAGTTGTCATGCTTGCGCTCCTTGCGTTGGGTTGCGACGTTCTTCGAAGTTAAGACAGACGCGCAAAATGtt                                                                                 >  2:1326827/1‑100 (MQ=255)        aCATATCCATGACGTTTTTGCGGACCTGAGTTGTCATGCTTGCGCTCCTTGCGTTGGGTTGCGACGTTCTTCGAAGTTAAGACAGACGCGCAAAATGTTg                                                                                <  1:633197/100‑1 (MQ=255)        aCATATCCATGACGTTTTTGCGGACCTGAGTTGTCATGCTTGCGCTCCTTGCGTTGGGTTGCGACGTTCTTCGAAGTTAAGACAGACGCGCAAAATGTTg                                                                                <  1:1373659/100‑1 (MQ=255)        aCATATCCATGACGTTTTTGCGGACCTGAGTTGTCATGCTTGCGCTCCTTGCGTTGGGTTGCGACGTTCTTCGAAGTTAAGACAGACGCGCAAAATGTTg                                                                                <  1:174256/100‑1 (MQ=255)        aCATATCCATGACGTTTTTGCGGACAAGAGTTGTCTTGCTTGCGCTCCTTGCGTTGGGTTGCGACGTTCTTCGAAGTTAAGACAGACGCGTAAAATGttc                                                                                <  2:543476/100‑2 (MQ=255)          atatCCATGACGTTTTTGCGGACCTGAGTTGTCATGCTTGCGCTCCTTGCGTTGGGTTGCGACGTTCTTCGAAGTTAAGACAGACGCGCAAAATGTTGGc                                                                              <  2:1218442/100‑1 (MQ=255)           tatCCATGACGTTTTTGCGGACCTGAGTTGTCATGCTTGCGCTCCTTGCGTTGGGTTGCGACGTTCTTCGAAGTTAAGACAGACGCGCAAAATGTTGGCt                                                                             >  1:1233728/1‑100 (MQ=255)            atCCATGACGTTTTTGCGGACCTGAGTTGTCATGCTTGCGCTCCTTGCGTTGGGTTGCGACGTTCTTCGAAGTTAAGACAGACGCGCAAAATGTTGGCTc                                                                            <  1:1175778/100‑1 (MQ=255)             tCCATGACGTTTTTGCGGACCTGAGTTGTCATGCTTGCGCTCCTTGCGTTGGGTTGCGACGTTCTTCGAAGTTAAGACAGACGCGCAAAATGTTGGCTcc                                                                           <  1:66927/100‑1 (MQ=255)               cATGACGTTTTTGCGGACCTGAGTTGTCATGCTTGCGCTCCTTGCGTTGGGTTGCGACGTTCTTCGAAGTTAAGACAGACGCGCAAAATGTTGGCTCCGa                                                                         >  2:940212/1‑100 (MQ=255)               cATGACGTTTTTGCGGACCTGAGTTGTCATGCTTGCGCTCCTTGCGTTGGGTTGCGACGTTCTTCGAAGTTAAGACAGACGCGCAAAATGTTGGCTCCGa                                                                         >  2:340568/1‑100 (MQ=255)                 tGACGTTTTTGCGGACCTGAGTTGTCATGCTTGCGCTCCTTGCGTTGGGTTGCGACGTTCTTCGAAGTTAAGACAGACGCGCAAAATGTTGGCTCCGACa                                                                       >  1:3065/1‑100 (MQ=255)                    cGTTTTTGCGGACCTGAGTTGTCATGCTTGCGCTCCTTGCGTTGGGTTGCGACGTTCTTCGAAGTTAAGACAGACGCGCAAAATGTTGGCTCCGACAAAt                                                                    <  2:1558952/100‑1 (MQ=255)                    cGTTTTTGCGGACCTGAGTTGTCATGCTTGCGCTCCTTGCGTTGGGTTGCGACGTTCTTCGAAGTTAAGACAGACGCGCAAAATGTTGGCTCCGACAAAt                                                                    <  1:118885/100‑1 (MQ=255)                     gTTTTTGCGGACCTGAGTTGTCATGCTGGCGCTCCTTGCGTTGGGTTGCGACGTTCTTCGAAGTTAAGACAGACGCGCAAAATGTTGTCTCCGACAAAtt                                                                   >  1:464065/1‑100 (MQ=255)                        tttGCGGACCTGAGTTGTCATGCTTGCGCTCCTTGCGTTGGGTTGCGACGTTCTTCGAAGTTAAGACAGACGCGCAAAATGTTGGCTCCGACAAATTTaa                                                                <  2:1014799/100‑1 (MQ=255)                         ttGCGGACCTGAGTTGTAATGCTTTCGCTCCTTGCGTTGGGTTGCGACGTGCTTCGAAGTTAAGACAGACGCGCAAAATGTTGGCTCCGACAAATTTaaa                                                               <  2:1455677/100‑1 (MQ=255)                          tGCGGACCTGAGTTGTCATGCTTGCGCTCCTTGCGTTGGGTTGCGACGTTCTTCGAAGTTAAGACAGACGCGCAAAATGTTGGCTCCGACAAATTTAAac                                                              >  2:1536024/1‑100 (MQ=255)                             ggACCTGAGTTGTCATGCTTGCGCTCCTTGCGTTGGGTTGCGACGTTCTTCGAAGTTAAGACAGACGCGCAAAATGTTGGCTCCGACAAATTTAAACAcc                                                           >  1:1564283/1‑100 (MQ=255)                               aCCTGAGTTGTCATGCTTGCGCTCCTTGCGTTGGGTTGCGACGTTCTTCGAAGTTAAGACAGACGCGCAAAATGTTGGCTCCGACAAATTTAAACACCAg                                                         <  1:465669/100‑1 (MQ=255)                                ccTGAGTTGTCATGCTTGCGCTCCTTGCGTTGGGTTGCGACGTTCTTCGAAGTTAAGACAGACGCGCAAAATGTTGGCTCCGACAAATTTAAACACCAgg                                                        >  1:1466474/1‑100 (MQ=255)                                 cTGAGTTGTCATGCTTGCGCTCCTTGCGTTGGGTTGCGACGTTCTTCGAAGTTAAGACAGACGCGCAAAATGTTGGCTCCGACAAATTTAAACACCAg                                                         <  2:1017465/98‑1 (MQ=255)                                 cTGAGTTGTCATGCTTGCGCTCCTTGCGTTGGGTTGCGACGTTCTTCGAAGTTAAGACAGACGCGCAAAATGTTGGCTCCGACAAATTTAAACACCAGGa                                                       >  1:23591/1‑100 (MQ=255)                                  tGAGTTGTCATGCTTGCGCTCCTTGCGTTGGGTTGCGACGTTCTTCGAAGTTAAGACAGACGCGCAAAATGTTGGCTCCGACAAATTTAAACACCAGGAt                                                      >  1:909354/1‑100 (MQ=255)                                  tGAGTTGTCATGCTTGCGCTCCTTGCGTTGGGTTGCGACGTTCTTCGAAGTTAAGACAGACGCGCAAAATGTTGACTCCGACAAAATTAAACACGAGGAt                                                      >  2:382516/1‑100 (MQ=255)                                     gTTGTCATGCTTGCGCTCCTTGCGTTGGGTTGCGACGTTCTTCGAAGTTAAGACAGACGCGCAAAATGTTGGCTCCGACAAATTTAAACACCAGGATGAc                                                   >  1:778977/1‑100 (MQ=255)                                      ttGTCATGCTTGCGCTCCTTGCGTTGGGTTGCGACGTTCTTCGAAGTTAAGACAGACGCGCAAAATGTTGGCTCCGACAAATTTAAACACCAGGATGACg                                                  <  1:1026582/100‑1 (MQ=255)                                        gTCATGCTTGCGCTCCTTGCGTTGGGTTGCGACGTTCTTCGAAGTTAAGACAGACGCGCAAAATGTTTGCTCCGACAAATGTAAACACCAGGATGACgg                                                 >  2:125327/1‑99 (MQ=255)                                          cATGCTTGCGCTCCTTGCGTTGGGTTGCGACGTTCTTCGAAGTTAAGACAGACGCGCAAAATGTTGGCTCCGACAAATTTAAACACCAGGATGACGGCTa                                              >  1:379328/1‑100 (MQ=255)                                          cATGCTTGCGCTCCTTGCGTTGGGTTGCGACGTTCTTCGAAGTTAAGACAGACGCGCAAAATGTTGGCTCCGACAAATTTAAACACCAGGATGACGGCTa                                              >  1:532575/1‑100 (MQ=255)                                             gCTTGCGCTCCTTGCGTTGGGTTGCGACGTTCTTCGAAGTTAAGACAGACGCGCAAAATGTTGGCTCCGACAAATTTAAACACCAGGATGACGGCTAAAg                                           <  1:415536/100‑1 (MQ=255)                                               ttGCGCTCCTTGCGTTGGGTTGCGACGTTCTTCGAAGTTAAGACAGACGCGCAAAATGTTGGCTCCGACAAATTTAAACACCAGGATGACGGCTAAAGGt                                         >  1:1236859/1‑100 (MQ=255)                                                tGCGCTCCTTGCGTTGGGTTGCGACGTTCTTCGAAGTTAAGACAGACGCGCAAAATGTTGGCTCCGACAAATTTAAACACCAGGATGACGGCTAAAGGTa                                        >  2:901046/1‑100 (MQ=255)                                                tGCGCTCCTTGCGTTGGGTTGCGACGTTCTTCGAAGTTAAGACAGACGCGCAAAATGTTGGCTCCGACAAATTTAAACACCAGGATGACGGCTAAAGGTa                                        >  1:1083419/1‑100 (MQ=255)                                                 gcgcTCCTTGCGTTGGGTTGCGACGTTCTTCGAAGTTAAGACAGACGCGCAAAATGTTGGCTCCGACAAATTTAAACACCAGGATGACGACTAAAGGTAc                                       <  1:1260227/100‑1 (MQ=255)                                                   gcTCCTTGCGTTGGGTTGCGACGTTCTTCGAAGTTAAGACAGACGCGCAAAATGTTGGCTCCGACAAATTTAAACACCAGGATGACGGCTAAAGGTACGa                                     <  2:1233721/100‑1 (MQ=255)                                                     tCCTTGCGTTGGGTTGCGACGTTCTTCGAAGTTAAGACAGACGCGCAAAATGTTGGCTCCGACAAATTTAAACACCAGGATGACGGCTAAAGGTACGAtt                                   <  1:940220/100‑1 (MQ=255)                                                        ttGCGTTGGGTTGCGACGTTCTTCGAAGTTAAGACAGACGCGCAAAATGTTGGCTCCGACAAATTTAAACACCAGGATGACGGCTAAAGGTACGATTACg                                <  2:3065/100‑1 (MQ=255)                                                         tGCGTTGGGTTGCGACGTTCTTCGAAGTTAAGACAGACGCGCAAAATGTTGGCTCCGACAAATTTAAACACCAGGATGACGGCTAAAGGTACGATTACgg                               <  1:179144/100‑1 (MQ=255)                                                           cGTTGGGTTGCGACGTTCTTCGAAGTTAAGACAGACGCGCAAAATGTTGGCTCCGACAAATTTAAACACCAGGATGACGGCTAAAGGTACGATTACGGAc                             <  1:410308/100‑1 (MQ=255)                                                               gggTTGCGACGTTCTTCGAAGTTAAGACAGACGCGCAAAATGTTGGCTCCGACAAATTTAAACACCAGGATGACGGCTAAAGGTACGATTACGGACGTGc                         >  1:473351/1‑100 (MQ=255)                                                                ggTTGCGACGTTCTTCGAAGTTAAGACAGACGCGCAAAATGTTGGCTCCGACAAATTTAAACACCAGGATGACGGCTAAAGGTACGATTACGGACGTTcc                        >  2:685915/1‑100 (MQ=255)                                                                ggTTGCGACGTTCTTCGAAGTTAAGACAGACGCGCAAAATGTTGGCTCCGACAAATTTAAACACCAGGATGACGGCTAAAGGTACGATTACGGACGTGcc                        <  2:1142109/100‑1 (MQ=255)                                                                ggTTGCGACGTTCTTCGAAGTTAAGACAGACGCGCAAAATGTTGGCTCCGACAAATTTAAACACCAGGATGACGGCTAAAGGTACGATTACGGACGTGcc                        >  1:595522/1‑100 (MQ=255)                                                                ggTTGCGACGTTCTTCGAAGTTAAGACAGACGCGCAAAATGTTGGCTCCGACAAATTTAAACACCAGGATGACGGCTAAAGGTACGATTACGGACGTGcc                        <  1:608720/100‑1 (MQ=255)                                                                 gTTGCGACGTTCTTCGAAGTTAAGACAGACGCGCAAAATGTTGGCTCCGACAAATTTAAACACCAGGATGACGGCTAAAGGTACGATTACGGACGTGccc                       <  1:1010097/100‑1 (MQ=255)                                                                 gTTGCGACGTTCTTCGAAGTTAAGACAGACGCGCAAAATGTTGGCTACGACAAATTTAAACACCAGGATGACGGCTAAAGGTACGATTACGGACGTGccc                       <  2:464062/100‑1 (MQ=255)                                                                    gCGACGTTCTTCGAAGTTAAGACAGACGCGCAAAATGTTGGCTCCGACAAATTTAAACACCAGGATGACGGCTAAAGGTACGATTACGGACGTGCCCAGa                    >  1:39047/1‑100 (MQ=255)                                                                        cGTTCTTCGAAGTTAAGACAGACGCGCAAAATGTTGGCTCCGACAAATTTAAACACCAGGATGACGGCTAAAGGTACGATTACGGACGTGCCCAGAAAgg                <  1:209201/100‑1 (MQ=255)                                                                               cGAAGTTAAGACAGACGCGCAAAATGTTGGCTCCGACAAATTTAAACACCAGGATGACGGCTAAAGGTACGATTACGGACGTGCCCAGAAAGGCAAacac         <  2:456987/100‑1 (MQ=255)                                                                                  aGTTAAGACAGACGCGCAAAATGTTGGCTCCGACAAATTTAAACACCAGGATGACGGCTAAAGGTACGATTACGGACGTGCCCAGAAAGGCAAACACcgc      <  1:1442591/100‑1 (MQ=255)                                                                                  aGTTAAGACAGACGCGCAAAATGTTGGCTCCGACAAATTTAAACACCAGGATGACGGCTAAAGGTACGATTACGGACGTGCCCAGAAAGGCAAACACcgc      <  2:217137/100‑1 (MQ=255)                                                                                  aGTTAAGACAGACGCGCAAAATGTTGGCTCCGACAAATTTAAACACCAGGATGACGGCTAAAGGTACGATTACGGACGTGCCCAGAAAGGCAAACACcgc      <  1:189794/100‑1 (MQ=255)                                                                                  aGTTAAGACAGACGCGCAAAATGTTGGCTCCGACAAATTTAAACACCAGGATGACGGCTAAAGGTACGATTACGGACGTGCCCAGAAAGGCAAACACcgc      <  1:978894/100‑1 (MQ=255)                                                                                     tAAGACAGACGCGCAAAATGTTGGCTCCGACAAATTTAAACACCAGGATCACGGCTAAAGGTACGATTACGGACGTGCCCAGAAAGGCAAACACCGCGAc   >  2:306512/1‑100 (MQ=255)                                                                                      aaGACAGACGCGCAAAATGTTGGCTCCGACAAATTTAAACACCAGGATGACGGCTAAAGGTACGATTACGGACGTGCCCAGAAAGGCAAACACCGCGACg  <  2:32533/100‑1 (MQ=255)                                                                                         |                                                                                                 TCGATAAACATATCCATGACGTTTTTGCGGACCTGAGTTGTCATGCTTGCGCTCCTTGCGTTGGGTTGCGACGTTCTTCGAAGTTAAGCCAGACGCGCAAAATGTTGGCTCCGACAAATTTAAACACCAGGATGACGGCTAAAGGTACGATTACGGACGTGCCCAGAAAGGCAAACACCGCGACG  >  NC_000913/4559957‑4560141

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑