Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,615,859	T→G	T403P (ACC→CCC)	yjjI ←	DUF3029 family protein, putative glycine radical enzyme

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,615,859	0	T	G	100.0%	188.9 / NA	53	T403P (ACC→CCC)	yjjI	DUF3029 family protein, putative glycine radical enzyme
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (25/28);  total (25/28)

CCTGCGGGTTACGTTTGATGGTTTCGTCGAGCGTCAGAATGTCGCTGATGCCGGAATAATAATAAGCATGATGCGGGGCGACAGTTTGCAGATGGGTGATCGGATCTGGCTCATCGCCATACGGCAAACGCGCGCCCGGCGTGGTGCCGATATCGGAACTGATCCCCGACTGTGCGTGTAACATGGCGCGTTT  >  NC_000913/4615763‑4615955                                                                                                 |                                                                                                 ccTGCGGGTTACGTTTGATGGTTTCGTCGAGCGTCAGAATGTCGCTGATGCCGGAATAATAATAAGCATGATGCGGGGCGACAGTTTGCAGATGGGGGAt                                                                                               <  2:323867/100‑1 (MQ=255)  cTGCGGGTTACGTTTGATGGTTTCGTCGAGCGTCAGAATGTCGCTGATGCCGGAATAATAATAAGCATGATGCGGGGCGACAGTTTGCAGATGGGGGATc                                                                                              <  2:437113/100‑1 (MQ=255)   tGCGGGTTACGTTTGATGGTTTCGTCGAGCGTCAGAATGTCGCTGATGCCGGAATAATAATAAGCATGATGCGGGGCGACAGTTTGCAGATGGGGGATCg                                                                                             <  2:785681/100‑1 (MQ=255)    gCGGGTTACGTTTGATGGTTTCGTCGAGCGTCAGAATGTCGCTGATGCCGGAATAATAATAAGCATGATGCGGGGCGACAGTTTGCAGATGGGGGATCgg                                                                                            <  2:503167/100‑1 (MQ=255)          tACGTTTGATGGTTTCGTCGAGCGTCAGAATGTCGCTGATGCCGGAATAATAATAAGCATGATGCGGGGCGACAGTTTGCAGATGGGGGATCGGATCTgg                                                                                      <  1:1237990/100‑1 (MQ=255)           aCGTTTGATGGTTTCGTCGAGCGTCAGAATGTCGCTGATGCCGGAATAATAATAAGCATGATGCGGGGCGACAGTTTGCAGATGGGGGATCGGATCTGGc                                                                                     <  1:356049/100‑1 (MQ=255)             gTTTGATGGTTTCGTCGAGCGTCAGAATGTCGCTGATGCCGGAATAATAATAAGCATGATGCGGGGCGACAGTTTGCAGATGGGGGATCGGATCTGGCTc                                                                                   >  1:643632/1‑100 (MQ=255)                 gATGGTTTCGTCGAGCGTCAGAATGTCGCTGATGCCGGAATAATAATAAGCATGATGCGGGGCGACAGTTTGCAGATGGGGGATCGGATCTGGCTCATCg                                                                               >  2:1479478/1‑100 (MQ=255)                  aTGGTTTCGTCGAGCGTCAGAATGTCGCTGATGCCGGAATAATAATAAGCATGATGCGGGGCGACAGTTTGCAGATGGGGGATCGGATCTGGCTCATCGc                                                                              <  1:370595/100‑1 (MQ=255)                    ggTTTCGTCGGGCGTCAGAATGTCGCTGATGCCGGAATAATAATAAGCATGATGCGGGGCGACAGTTTGCAGATGGGGGATCGGATCTGGCTCATCGCCa                                                                            <  2:1483884/100‑1 (MQ=255)                     gTTTCGTCGAGCGTCAGAATGTCGCTGATGCCGGAATAATAATAAGCATGATGCGGGGCGACAGTTTGCAGATGGGGGATCGGATCTGGCTCATCGCCAt                                                                           >  1:888159/1‑100 (MQ=255)                      tttCGTCGAGCGTCAGAATGTCGCTGATGCCGGAATAATAATAAGCATGATGCGGGGCGACAGTTTGCAGATGGGGGATCGGATCTGGCTCATCGCCATa                                                                          <  1:662337/100‑1 (MQ=255)                       ttCGTCGAGCGTCAGAATGTCGCTGATGCCGGAATAATAATAAGCATGATGCGGGGCGACAGTTTGCAGATGGGGGATCGGATCTGGCTCATCGCCATAc                                                                         >  2:670981/1‑100 (MQ=255)                       ttCGTCGAGCGTCAGAATGTCGCTGATGCCGGAATAATAATAAGCATGATGCGGGGCGACAGTTTGCAGATGGGGGATCGGATCTGGCTCATCGCCATAc                                                                         <  1:380742/100‑1 (MQ=255)                         cgtcgAGCGTCAGAATGTCGCTGATGCCGGAATAATAATAAGCATGATGCGGGGCGACAGTTTGCAGATGGGGGATCGGATCTGGCTCATCGCCATACgg                                                                       <  2:370883/100‑1 (MQ=255)                         cgtcgAGCGTCAGAATGTCGCTGATGCCGGAATAATAATAAGCATGATGCGGGGCGACAGTTTGCAGATGGGGGATCGGATCTGGCTCATCGCCATACgg                                                                       <  2:91104/100‑1 (MQ=255)                          gtcgAGCGTCAGAATGTCGCTGATGCCGGAATAATAATAAGCATGATGCGGGGCGACAGTTTGCAGATGGGGGATCGGATCTGGCTCATCGCCATACGGc                                                                      <  1:1166935/100‑1 (MQ=255)                            cgAGCGTCAGAATGTCGCTGATGCCGGAATAATAATAAGCATGATGCGGGGCGACAGTTTGCAGATGGGGGATCGGATCTGGCTCATCGCCATACGGCaa                                                                    >  1:1032729/1‑100 (MQ=255)                              aGCGTCAGAATGTCGCTGATGCCGGAATAATAATAAGCATGATGCGGGGCGACAGTTTGCAGATGGGGGATCGGATCTGGCTCATCGCCATACGGCAAAc                                                                  <  1:99322/100‑1 (MQ=255)                                     gAATGTCGCTGATGCCGGAATAATAATAAGCATGATGCGGGGCGACAGTTTGCAGATGGGGGATCGGATCTGGCTCATCGCCATACGGCAAACGCGCGcc                                                           <  2:1227364/100‑1 (MQ=255)                                         gTCGCTGATGCCGGAATAATAATAAGCATGATGCGGGGCGACAGTTTGCAGATGGGGGATCGGATCTGGCTCATCGCCATACGGCAAACGCGCGCCCGGc                                                       <  1:405136/100‑1 (MQ=255)                                          tCGCTGATGCCGGAATAATAATAAGCATGATGCGGGGCGACAGTTTGCAGATGGGGGATCGGATCTGGCTCATCGCCATACGGCAAACGCGCGCCCggtg                                                      >  1:82429/1‑98 (MQ=255)                                                 tGCCGGAATAATAATAAGCATGATGCGGGGCGACAGTTTGCAGATGGGGGATCGGATCTGGCTCATCGCCATACGGCAAACGCGCGCCCGGCGTGGTGcc                                               >  2:576431/1‑100 (MQ=255)                                                 tGCCGGAATAATAATAAGCATGATGCGGGGCGACAGTTTGCAGATGGGGGATCGGATCTGGCTCATCGCCATACGGCAAACGCGCGCCCGGCGTGGTGcc                                               >  2:1364557/1‑100 (MQ=255)                                                  gCCGGAATAATAATAAGCATGATGCGGGGCGACAGTTTGCAGATGGGGGATCGGATCTGGCTCATCGCCATACGGCAAACGCGCGCCCGGCGTGGTGcc                                               >  2:414676/1‑99 (MQ=255)                                                  gCCGGAATAATAATAAGCATGATGCGGGGCGACAGTTTGCAGATGGGGGATCGGATCTGGCTCATCGCCATACGGCAAACGCGCGCCCGGCGTGGTGCCg                                              >  2:1012324/1‑100 (MQ=255)                                                         taataataaGCATGATGCGGGGCGACAGTTTGCAGATGGGGGATCGGATCTGGCTCATCGCCATACGGCAAACGCGCGCCCGGCGTGGTGCCGATATCgg                                       >  1:1316378/1‑100 (MQ=255)                                                          aataataaGCATGATGCGGGGCGACAGTTTGCAGATGGGGGATCGGATCTGGCTCATCGCCATACGGCAAACGCGCGCCCGGCGTGGTGCCGATATCgg                                       <  1:226869/99‑1 (MQ=255)                                                           ataataaGCATGATGCGGGGCGACAGTTTGCAGATGGGGGATCGGATCTGGCTCATCGCCATACGGCAAACGCGCGCCCGGCGTGGTGCCGATATCGGaa                                     <  1:459159/100‑1 (MQ=255)                                                             aataaGCATGATGCGGGGCGACAGTTTGCAGATGGGGGATCGGATCTGGCTCATCGCCATACGGCAAACGCGCGCCCGGCGTGGTGCCGATATCGGAACt                                   <  1:1048752/100‑1 (MQ=255)                                                               tcaGCAGGCTGCGGGGCGACAGTTTGCAGATGGGGGATCGGATCTGGCTCATCGCCATACGGCAAACGCGCGCCCGGTGTGGTGCCGATATCGGAACTGa                                 <  2:380313/98‑1 (MQ=255)                                                               taaGCATGATGCGGGGCGACAGTTTGCAGATGGGGGATCGGATCTGGCTCATCGCCATACGGCAAACGCGCGCCCGGCGTGGTGCCGATATCGGAACTGa                                 >  1:1356658/1‑100 (MQ=255)                                                               taaGCATGATGCGGGGCGACAGTTTGCAGATGGGGGATCGGATCTGGCTCATCGCCATACGGCAAACGCGCGCCCGGCGTGGTGCCGATATCGGAACTGa                                 >  1:406737/1‑100 (MQ=255)                                                                aaGCATGATGCGGGGCGACAGTTTGCAGATGGGGGATCGGATCTGGCTCATCGCCATACGGCAAACGCGCGCCCGGCGTGGTGCCGATATCGGAACTGAt                                <  2:125216/100‑1 (MQ=255)                                                                  gCATGATGCGGGGCGACAGTTTGCAGATGGGGGATCGGATCTGGCTCATCGCCATACGGCAAACGCGCTCCCGGCGTGGTGCCGATATCGGAACTGCTcc                              >  2:290779/1‑100 (MQ=255)                                                                   cATGATGCGGGGCGACAGTTTGCAGATGGGGGATCGGATCTGGCTCATCGCCATACGGCAAACGCGCGCCCGGCGTGGTGCCGATATCGGAACTGATccc                             <  2:285410/100‑1 (MQ=255)                                                                    atgatgCGGGGCGACAGTTTGCAGATGGGGGATGGGATCTGGCTCATCGCCATACGGCAAACGCGCGCCCGGCGTGGTGCCGATATCGGAACTGATccn                             <  1:1291301/99‑2 (MQ=255)                                                                         gCGGGGCGACAGTTTGCAGATGGGGGATCGGATCTGGCTCATCGCCATACGGCAAACGCGCGCCCGGCGTGGTGCCGATATCGGAACTGATCCCCGACtg                       >  2:1237102/1‑100 (MQ=255)                                                                         gCGGGGCGACAGTTTGCAGATGGGGGATCGGATCTGGCTCATCGCCATACGGCAAACGCGCGCCCGGCGTGGTGCCGATATCGGAACTGATCCCCGACtg                       <  2:1432125/100‑1 (MQ=255)                                                                          cGGGGCGACAGTTTGCAGATGGGGGATCGGATCTGGCTCATCGCCATACGGCAAACGCGCGCCCGGCGTGGTGCCGATATCGGAACTGATcccc                            >  2:1078816/1‑94 (MQ=255)                                                                           ggggCGACAGTTTGCAGATGGGGGATCGGATCTGGCTCATCGCCATACGGCAAACGCGCGCCCGGCGTGGTGCCGATATCGGAACTGATCCCCGACtgtg                     <  2:921830/100‑1 (MQ=255)                                                                           ggggCGACAGTTTGCAGATGGGGGATCGGATCTGGCTCATCGCCATACGGCAAACGCGCGCCCGGCGTGGTGCCGATATCGGAACTGATCCCCGACtgtg                     <  2:922169/100‑1 (MQ=255)                                                                              gCGACAGTTTGCAGATGGGGGATCGGATCTGGCTCATCGCCATACGGCAAACGCGCGCCCGGCGTGGTGCCGATATCGGAACTGATCCCCGACTGTGCgt                  >  2:1427824/1‑100 (MQ=255)                                                                              gCGACAGTTTGCAAATGGGGGATCGGATCTGGCTCATCGCCATACGGCAAACGCGCGCCCGGCGTGGTGCCGATATCGGAACTGATCCCCGACTGTGCgt                  >  2:972647/1‑100 (MQ=255)                                                                               cGACAGTTTGCAGATGGGGGATCGGATCTGGCTCATCGCCATACGGCAAACGCGCGCCCGGCGTGGTGCCGATATCGGAACTGATCCCCGACTGTGCgtg                 >  1:1524118/1‑100 (MQ=255)                                                                                 aCAGTTTGCAGATGGGGGATCGGATCTGGCTCATCGCCATACGGCAAACGCGCGCCCGGCGTGGTGCCGATATCGGAACTGATCCCCGACTGTGCGTGTa               >  1:596235/1‑100 (MQ=255)                                                                                 aCAGTTTGCAGATGGGGGATCGGATCTGGCTCATCGCCATACGGCAAACGCGCGCCCGGCGTGGTGCCGATATCGGAACTGATCCCCGACTGTGCGTGTa               >  1:1542804/1‑100 (MQ=255)                                                                                  gatcctGCAGATGGGGGATCGGATCTGGCTCATCGCCATACGGCAAACGCGCGCCCGGCGTGGTGCCGATATCGGAACTGATCCCCGACTGTGCGTGTaa              <  1:968104/95‑1 (MQ=255)                                                                                   aGTTTGCAGATGGGGGATCGGATCTGGCTCATCGCCATACGGCAAACGCGCGCCCGGCGTGGTGCCGATATCGGAACTGATCCCCGACTGTGCGTGTAAc             >  1:1161938/1‑100 (MQ=255)                                                                                     tttGCAGATGGGGGATCGGATCTGGCTCATCGCCATACGGCAAACGCGCGCCCGGCGTGGTGCCGATATCGGAACTGATCCCCGACTGTGCGTGTAACAt           <  1:661689/100‑1 (MQ=255)                                                                                       tGCAGATGGGGGAACGGATCTGGCTCATCGCCATACGGAAAACGCGCGCCCGGCGTGGTGCCGATATCGGAACTGATCCCCGACTGTGCGTGTaaagatc         >  2:968096/1‑95 (MQ=255)                                                                                        gCAGATGGGGGATCGGATCTGGCTCATCGCCATACGGCAAACGCGCGCCCGGCGTGGTGCCGATATCGGAACTGATCCCCGACTGTGCGTGTAACATGgc        >  1:892572/1‑100 (MQ=255)                                                                                          aGATGGGGGATCGGATCTGGCTCATCGCCATACGGCAAACGCGCGCCCGGCGTGGTGCCGATATCGGAACTGATCCCCGACTGTGCGTGTAACATTgcgc      >  2:754120/1‑100 (MQ=255)                                                                                              ggggGATCGGATCTGGCTCATCGCCATACGGCAAACGCGCGCCCGGCGTGGTGCCGATATCGGAACTGATCCCCGACTGTGCGTGTAACATGGCGCGttt  >  2:877336/1‑100 (MQ=255)                                                                                                 |                                                                                                 CCTGCGGGTTACGTTTGATGGTTTCGTCGAGCGTCAGAATGTCGCTGATGCCGGAATAATAATAAGCATGATGCGGGGCGACAGTTTGCAGATGGGTGATCGGATCTGGCTCATCGCCATACGGCAAACGCGCGCCCGGCGTGGTGCCGATATCGGAACTGATCCCCGACTGTGCGTGTAACATGGCGCGTTT  >  NC_000913/4615763‑4615955

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑