Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,631,700 T→C L323P (CTT→CCT)  slt → lytic murein transglycosylase, soluble

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,631,7000TC96.8% 218.4 / ‑1.8 63L323P (CTT→CCT) sltlytic murein transglycosylase, soluble
Reads supporting (aligned to +/- strand):  ref base T (2/0);  new base C (25/36);  total (27/36)
Fisher's exact test for biased strand distribution p-value = 1.80e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.15e-01

GGCAACGATGTCACCGACGAGCAGGCGAAATGGCGCGATGACGCCATTATGCGCTCGCAATCTACTTCGCTTATTGAACGCCGTGTACGAATGGCGCTTGGCACCGGCGATCGTCGCGGCCTGAATACCTGGCTGGCGCGTCTGCCTATGGAAGCGAAAGAGAAAGATGAATGGCGTTACTGGCAGGCGGAT  >  NC_000913/4631603‑4631794
                                                                                                 |                                                                                              
ggCAACGATGTCACCGACGAGCAGGCGAAATGGCGCGATGACGCCATTATGCGCTCGCAATCTACTTCGCTTATTGAACGCCGTGTACGAATGGCGCCTg                                                                                              <  2:1177468/100‑1 (MQ=255)
ggCAACGATGTCACCGACGAGCAGGCGAAATGGCGCGATGACGCCATTATGCGCTCGCAATCTACTTCGCTTATTGAACGCCGTGTACGAATGGCGCCTg                                                                                              <  2:364306/100‑1 (MQ=255)
  cAACGATGTCACCGACGAGCAGGCGAAATGGCGCGATGACGCCATTATGCGCTCGCAATCTACTTCGCTTATTGAACGCCGTGTACGAATGGCGCCTGGc                                                                                            <  2:1562877/100‑1 (MQ=255)
  cAACGATGTCACCGACGAGCAGGCGAAATGGCGCGATGACGCCATTATGCGCTCGCAATCTACTTCGCTTATTGAACGCCGTGTACGAATGGCGCCTGGc                                                                                            >  1:943178/1‑100 (MQ=255)
   aaCGATGTCACCGACGAGCAGGCGAAATGGCGCGATGACGCCATTATGCGCTCGCAATCTACTTCGCTTATTGAACGCCGTGTACGAATGGCGCCTGGCa                                                                                           >  2:1488693/1‑100 (MQ=255)
       aTGTCACCGACGAGCAGGCGAAATGGCGCGATGACGCCATTATGCGCTCGCAATCTACTTCGCTTATTGAACGCCGTGTACGAATGGCGCCTGGCACCgg                                                                                       <  1:685259/100‑1 (MQ=255)
        tGTCACCGACGAGCAGGCGAAATGGCGCGATGACGCCATTATGCGCTCGCAATCTACTTCGCTTATTGAACGCCGTGTACGAATGGCGCCTGGCACCGGc                                                                                      <  2:28507/100‑1 (MQ=255)
          tCACCGACGAGCAGGCGAAATGGCGCGATGACGCCATTATGCGCTCGCAATCTACTTCGCTTATTGAACGCCGTGTACGAATGGCGCCTGGCACCGGCGa                                                                                    >  1:328632/1‑100 (MQ=255)
           cACCGACGAGCAGGCGAAATGGCGCGATGACGCCATTATGCGCTCGCAATCTACTTCGCTTATTGAACGCCGTGTACGAATGGCGCCTGGCACCGGCGAt                                                                                   >  2:32965/1‑100 (MQ=255)
            aCCGACGAGCAGGCGAAATGGCGCGATGACGCCATTATGCGCTCGCAATCTACTTCGCTTATTGAACGCCGTGTACGAATGGCGCCTGGCACCGGCGAtc                                                                                  <  2:1307200/100‑1 (MQ=255)
            aCCGACGAGCAGGCGAAATGGCGCGATGACGCCATTATGCGCTCGCAATCTACTTCGCTTATTGAACGCCGTGTACGAATGGCGCCTGGCACCGGCGAtc                                                                                  <  2:1529934/100‑1 (MQ=255)
               gacgaGCAGGCGAAATGGCGCGATGACGCCATTATGCGCTCGCAATCTACTTCGCTTATTGAACGCCGTGTACGAATGGCGCCTGGCACCGGCGAtcgtc                                                                               >  1:151860/1‑100 (MQ=255)
               gacgaGCAGGCGAAATGGCGCGATGACGCCATTATGCGCTCGCAATCTACTTCGCTTATTGAACGCCGTGTACGAATGGCGCCTGGCACCGGCGAtcgtc                                                                               >  2:1289744/1‑100 (MQ=255)
                  gaGCAGGCGAAATGGCGCGATGACGCCATTATGCGCTCGCAATCTACTTCGCTTATTGAACGCCGTGTACGAATGGCGCCTGGCACCGGCGATCGTcgcg                                                                            <  1:171801/100‑1 (MQ=255)
                   aGCAGGCGAAATGGCGCGATGACGCCATTATGCGCTCGCAATCTACTTCGCTTATTGAACGCCGTGTACGAATGGCGCCTGGCACCGGCGATCGTCGCgg                                                                           <  1:1311525/100‑1 (MQ=255)
                    gCAGGCGAAATGGCGCGATGACGCCATTATGCGCTCGCAATCTACTTCGCTTATTGAACGCCGTGTACGAATGGCGCCTGGCACCGGCGATCGTCGCGGc                                                                          <  1:225488/100‑1 (MQ=255)
                       ggCGAAATGGCGCGATGACGCCATTATGCGCTCGCAATCTACTTCGCTTATTGAACGCCGTGTACGAATGACGCCTGGCACCGGCGATCGTCGCGGCCTg                                                                       >  1:113795/1‑100 (MQ=255)
                        gCGAAATGGCGCGATGACGCCATTATGCGCTCGCAATCTACTTCGCTTATTGAACGCCGTGTACGAATGGCGCCTGGCACCGGCGATCGTCGCGGCCTGa                                                                      >  1:608833/1‑100 (MQ=255)
                        gCGAAATGGCGCGATGACGCCATTATGCGCTCGCAATCTACTTCGCTTATTGAACGCCGTGTACGAATGGCGCCTGGCACCGGCGATCGTCGCGGCCTGa                                                                      <  2:50923/100‑1 (MQ=255)
                         cGAAATGGCGCGATGACGCCATTATGCGCTCGCAATCTACTTCGCTTATTGAACGCCGTGTACGAATGGCGCTTGGCACCGGCGATCGTCGCGGCCTGaa                                                                     >  1:1240161/1‑100 (MQ=255)
                         cGAAATGGCGCGATGACGCCATTATGCGCTCGCAATCTACTTCGCTTATTGAACGCCGTGTACGAATGGCGCCTGGCACCGGCGATCGTCGCGGCCTGa                                                                      <  2:240166/99‑1 (MQ=255)
                          gAAATGGCGCGATGACGCCATTATGCGCTCGCAATCTACTTCGCTTATTGAACGCCGTGTACGAATGGCGCCTGGCACCGGCGATCGTCGCGGCCTGAAt                                                                    <  1:183773/100‑1 (MQ=255)
                          gAAATGGCGCGATGACGCCATTATGCGCTCGCAATCTACTTCGCTTATTGAACGCCGTGTACGAATGGCGCCTGGCACCGGCGATCGTCGCGGCCTGAAt                                                                    <  2:171965/100‑1 (MQ=255)
                               ggCGCGATGACGCCATTATGCGCTCGCAATCTACTTCGCTTATTGAACGCCGTGTACGAATGGCGCCTGGCACCGGCGATCGTCGCGGCCTGAATACctg                                                               <  2:137143/100‑1 (MQ=255)
                               ggCGCGATGACGCCATTATGCGCTCGCAATCTACTTCGCTTATTGAACGCCGTGTACGAATGGCGCCTGGCACCGGCGATCGTCGCGGCCTGAATACctg                                                               <  1:524089/100‑1 (MQ=255)
                                gcgcGATGACGCCATTATGCGCTCGCAATCTACTTCGCTTATTGAACGCCGTGTACGAATGGCGCCTGGCACCGGCGATCGTCGCGGCCTGAATACctgg                                                              >  1:927021/1‑100 (MQ=255)
                                 cgcgATGACGCCATTATGCGCTCGCAATCTACTTCGCTTATTGAACGCCGTGTACGAATGGCGCCTGGCACCGGCGATNGTCNCGGCCTGAATACctggc                                                             >  2:887678/1‑100 (MQ=255)
                                        aCGCCATTATGCGCTCGCAATCTACTTCGCTTATTGAACGCCGTGTACGAATGGCGCCTGGCACCGGCGATCGTCGCGGCCTGAATACCTGGCTGGcgcg                                                      <  2:489074/100‑1 (MQ=255)
                                         cGCCATTATGCGCTCGCAATCTACTTCGCTTATTGAACGCCGTGTACGAATGGCGCCTGGCACCGGCGATCGTCGCGGCCTGAATACCTGGCTGGCGCGt                                                     >  2:1555886/1‑100 (MQ=255)
                                         cGCCATTATGCGCTCGCAATCTACTTCGCTTATTGAACGCCGTGTACGAATGGCGCCTGGCACCGGCGATCGTCGCGGCCTGAATACCTGGCTGGCGCGt                                                     >  1:693014/1‑100 (MQ=255)
                                            cATTATGCGCTCGCAATCTACTTCGCTTATTGAACGCCGTGTACGAATGGCGCCTGGCACCGGCGATCGTCGCGGCCTGAATACCTGGCTGGCGCGTCTg                                                  >  2:149301/1‑100 (MQ=255)
                                            cATTATGCGCTCGCAATCTACTTCGCTTATTGAACGCCGTGTACGAATGGCGCCTGGCACCGGCGATCGTCGCGGCCTGAATACCTGGCTGGCGCGTCTg                                                  >  1:426263/1‑100 (MQ=255)
                                              ttATGCGCTCGCAATCTACTTCGCTTATTGAACGCCGTGTACGAATGGCGCCTGGCACCGGCGATCGTCGCGGCCTGAATACCTGGCTGGCGCGTCTGcc                                                <  1:721042/100‑1 (MQ=255)
                                              ttATGCGCTCGCAATCTACTTCGCTTATTGAACGCCGTGTACGAATGGCGCCTGGCACCGGCGATCGTCGCGGCCTGAATACCTGGCTGGCGCGTCTGcc                                                <  1:968787/100‑1 (MQ=255)
                                              ttATGCGCTCGCAATCTACTTCGCTTATTGAACGCCGTGTACGAATGGCGCCTGGCACCGGCGATCGTCGCGGCCTGAATACCTGGCTGGCGCGTCTGcc                                                <  1:504776/100‑1 (MQ=255)
                                                 tGCGCTCGCAATCTACTTCGCTTATTGAACGCCGTGTACGAATGGCGCCTGGCACCGGCGATCGTCGCGGCCTGAATACCTGGCTGGCGCGTCTGCCTAt                                             <  1:1317792/100‑1 (MQ=255)
                                                  gcgcTCGCAATCTACTTCGCTTATTGAACGCCGTGTACGAATGGCGCCTGGCACCGGCGATCGTCGCGTCCTGAATACCTGGCTGGTGCGTCTGCCTATg                                            <  1:1058595/100‑1 (MQ=255)
                                                   cgcTCGCAATCTACTTCGCTTATTGAACGCCGTGTACGAATGGCGCCTGGCACCGGCGATCGTCGCGGCCTGAATACCTGGCTGGCGCGTCTGCCTATgg                                           <  1:924460/100‑1 (MQ=255)
                                                   cgcTCGCAATCTACTTCGCTCATTGAACGCCGTGTACGAATGGCGCCTGGCACCGGCGATCGTCGCGGCCTGAATACCTGGCTGGCGCGTCTGCCTATgg                                           >  2:959085/1‑100 (MQ=255)
                                                      tCGCAATCTACTTCGCTTATTGAACGCCGTGTACGAATGGCGCCTGGCACCGGCGATCGTCGCGGCCTGAATACCTGGCTGGCGCGTCTGCCTATGGAAg                                        <  1:766551/100‑1 (MQ=255)
                                                          aaTCTACTTCGCTTATTGAACGCCGTGTACGAATGGCGCCTGGCACCGGCGATCGTCGCGGCCTGAATACCTGGCTGGCGCGTCTGCCTATGGAAGCgaa                                    <  1:1184797/100‑1 (MQ=255)
                                                          aaTCTACTTCGCTTATTGAACGCCGTGTACGAATGGCGCCTGGCACCGGCGATCGTCGCGGCCTGAATACCTGGCTGGCGCGTCTGCCTATGGAAGCgaa                                    >  2:1083914/1‑100 (MQ=255)
                                                            tCTACTTCGCTTATTGAACGCCGTGTACGAATGGCGCCTGGCACCGGCGATCGTCGCGGCCTGAATACCTGGCTGGCGCGTCTGCCTATGGAAGCgaaag                                  <  2:426262/100‑1 (MQ=255)
                                                            tCTACTTCGCTTATTGAACGCCGTGTACGAATGGCGCCTGGCACCGGCGATCGTCGCGGCCTGAATACCTGGCTGGCGCGTCTGCCTATGGAAGCgaaag                                  <  1:1336224/100‑1 (MQ=255)
                                                             cTACTTCGCTTATTGAACGCCGTGTACGAATGGCGCCTGGCACCGGCGATCGTCGCGGCCTGAATACCTGGCTGGCGCGTCTGCCTATGGAAGCgaaaga                                 >  1:710674/1‑100 (MQ=255)
                                                             cTACTTCGCTTATTGAACGCCGTGTACGAATGGCGCCTGGCACCGGCGATCGTCGCGGCCTGAATACCTGGCTGGCGCGTCTGCCTATGGAAGCgaaaga                                 >  2:516081/1‑100 (MQ=255)
                                                                cTTCGCTTATTGAACGCCGTGTACGAATGGCGCTTGGCACCGGCGATCGTCGCGGCCTGAATACCTGGCTGGCGCGTCTGCCTATGGAAGCgaaagagaa                              >  1:15205/1‑100 (MQ=255)
                                                                cTTCGCTTATTGAACGCCGTGTACGAATGGCGCCTGGCACCGGCGATCGTCGCGGCCTGAATACCTGGCTGGCGCGTCTGCCTATGGAAGCgaaagagaa                              >  2:219639/1‑100 (MQ=255)
                                                                   cGCTTATTGAACGCCGTGTACGAATGGCGCCTGGCACCGGCGATCGTCGCGGCCTGAATACCTGGCTGGCGCGTCTGCCTATGGAAGCgaaagagaaaga                           >  2:1021343/1‑100 (MQ=255)
                                                                     cTTATTGAACGCCGTGTACGAATGGCGCCTGGCACCGGCGATCGTCGCGGCCTGAATACCTGGCTGGCGCGTCTGCCTATGGAAGCGAAAGAGAAAGATg                         >  2:1469247/1‑100 (MQ=255)
                                                                        aTTGAACGCCGTGTACGAATGGCGCCTGGCACCGGCGATCGTCGCGGCCTGAATACCTGGCTGGCGCGTCTGCCTATGGAAGCGAAAGAGAAAGATGAAt                      <  1:560992/100‑1 (MQ=255)
                                                                        aTTGAACGCCGTGTACGAATGGCGCCTGGCACCGGCGATCGTCGCGGCCTGAATACCTGGCTGGCGCGTCTGCCTATGGAAGCGAAAGAGAAAGATGAAt                      >  1:44489/1‑100 (MQ=255)
                                                                         ttGAACGCCGTGTACGAATGGCGCCTGGCACCGGCGATCGTCGCGGCCTGAATACCTGGCTGGCGCGTCTGCCTATGGAAGCGAAAGAGAAAGATGAATg                     <  1:1016776/100‑1 (MQ=255)
                                                                           gAACGCCGTGTACGAATGGCGCCTGGCACCGGCGATCGTAGCGGCCTGAATACCTGGCTGGCGCGTCTGCCTATGGAAGCGAAAGAGAAAGATGAATGGc                   <  1:706900/100‑1 (MQ=255)
                                                                             aCGCCGTGTACGAATGGCGCCTGGCACCGGCGATCGTCGCGGCCTGAATACCTGGCTGGCGCGTCTGCCTATGGAAGCGAAAGAGAAAGATGAATGGCGt                 >  2:78699/1‑100 (MQ=255)
                                                                                 cGTGTACGAATGGCGCCTGGCACCGGCGATCGTCGCGGCCTGAATACCTGGCTGGCGCGTCTGCCTATGGAAGCGAAAGAGAAAGATGAATGGCGTTACt             <  1:1555896/100‑1 (MQ=255)
                                                                                  gtgtACGAATGGCGCCTGGCACCGGCGATCGTCGCGGCCTGAATACCTGGCTGGCGCGTCTGCCTATGGAAGCGAAAGAGAAAGATGAATGGCGTTACTg            >  2:600845/1‑100 (MQ=255)
                                                                                    gtACGAATGGCGCCTGGCACCGGCGATCGTCGCGGCCTGAATACCTGGCTGGCGCGTCTGCCTATGGAAGCGAAAGAGAAAGATGAATGGCGTTACTGGc          <  2:765281/100‑1 (MQ=255)
                                                                                    gtACGAATGGCGCCTGGCACCGGCGATCGTCGCGGCCTGAATACCTGGCTGGCGCGTCTGCCTATGGAAGCGAAAGAGAAAGATGAATGGCGTTACTGGc          >  2:156308/1‑100 (MQ=255)
                                                                                       cGAATGGCGCCTGGCACCGGCGATCGTCGCGGCCTGAATACCTGGCTGGCGCGTCTGCCTATGGAAGCGAAAGAGAAAGATGAATGGCGTTACTGGCa         <  2:875971/98‑1 (MQ=255)
                                                                                          aTGGCGCCTGGCACCGGCGATCGTCGCGGCCTGAATACCTGGCTGGCGCGTCTGCCTATGGAAGCGAAAGAGAAAGATGAATGGCGTTACTGGCAGGCgg    <  2:1435765/100‑1 (MQ=255)
                                                                                           tGGCGCCTGGCACCGGCGATCGTCGCGGCCTGAATACCTGGCTGGCGCGTCTGCCTATGGAAGCGAAAGAGAAAGATGAATGGCGTTACTGGCAGGCGGa   <  1:32965/100‑1 (MQ=255)
                                                                                            ggCGCCTGGCACCGGCGATCGTCGCGGCCTGAATACCTGGCTGGCGCGTCTGCCTATGGAAGCGAAAGAGAAAGATGAATGGCGTTACTGGCAGGCGGAt  <  2:340797/100‑1 (MQ=255)
                                                                                                 |                                                                                              
GGCAACGATGTCACCGACGAGCAGGCGAAATGGCGCGATGACGCCATTATGCGCTCGCAATCTACTTCGCTTATTGAACGCCGTGTACGAATGGCGCTTGGCACCGGCGATCGTCGCGGCCTGAATACCTGGCTGGCGCGTCTGCCTATGGAAGCGAAAGAGAAAGATGAATGGCGTTACTGGCAGGCGGAT  >  NC_000913/4631603‑4631794

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: