| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 3,950,467 | 2 bp→TT | intergenic (+47/‑39) | ilvL → / → ilvX | ilvG operon leader peptide/uncharacterized protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,950,467 | 0 | G | T | 82.9% | 89.1 / 19.0 | 41 | intergenic (+47/‑40) | ilvL/ilvX | ilvG operon leader peptide/uncharacterized protein |
| Reads supporting (aligned to +/- strand): ref base G (2/5); new base T (17/17); total (19/22) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.19e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.04e-01 | |||||||||||
| * | NC_000913 | 3,950,468 | 0 | G | T | 97.5% | 129.0 / NA | 41 | intergenic (+48/‑39) | ilvL/ilvX | ilvG operon leader peptide/uncharacterized protein |
| Reads supporting (aligned to +/- strand): ref base G (1/0); major base T (18/21); minor base C (0/1); total (19/22) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TTATCCCACCGTGCGGGGCTGCACTTGGACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGGTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAACAGCACAAAATTCTGTTTCTCAAGATTCAGGACGGGGAACTAACT > NC_000913/3950377‑3950559 || ttatCCCACCGTGCGGGGCTGCACTTGGACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGtttttttttt > 1:788979/1‑100 (MQ=255) tatCCCACCGTGCGGGGCTGCACTTGGACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGTTTTTTTTTTg < 1:1425090/100‑1 (MQ=255) tatCCCACCGTGCGGGGCTGCACTTGGACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGTTTTTTTTTTg < 1:572074/100‑1 (MQ=255) atCCCACCGTGCGGGGCTGCACTTGGACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGTTTTTTTTTTGa < 1:904942/100‑1 (MQ=255) tCCCACCGTGCGGGGCTGCACTTGGACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGTTTTTTTTTTGAc > 1:1356332/1‑100 (MQ=255) tCCCACCGTGCGGGGCTGCACTTGGACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGTTTTTTTTCTGAc < 2:1197324/100‑1 (MQ=255) cccACCGTGCGGGGCTGCACTTGGCCGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGCCCCCCGCACCGAAAGGTCCGGGTTTTTTTTTTGAcc < 1:266522/100‑1 (MQ=255) cccACCGTGCGGGGCTGCACTTGGACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGTTTTTTTTTTGAcc < 1:287604/100‑1 (MQ=255) cACCGTGCGGGGCTGCACTTGGACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGTTTTTTTTTTTTCCtt > 2:1335598/1‑100 (MQ=255) ggggggGTGGAATTGGACGAGGAAAGGCGTAGAGATCAAGTCTTAACGAACTAAGACCCCCGAACCGAAAGGTCCGGGTTTTTTTTTTGACCTTaaaaa < 2:1290463/97‑1 (MQ=255) ggggGTGCACTTGGACGAGGACCGGCTGGGAGATCAAGCCTTAACGAACTAAGACCCCCAAAACGAAAGGTCCGGGTTTTTTTTTTGACCTTAAAAACa < 2:382789/99‑1 (MQ=255) ggggCTGCACTTGGACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCC‑GGGTTTTTTTTTGACCTTAAAAACATa < 2:749334/100‑1 (MQ=255) ggggCTGCACTTGGACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCC‑GGGTTTTTTTTTGACCTTAAAAACATa < 2:748931/100‑1 (MQ=255) tGCGCTTGGGCGAGGAAAGGCTCCGAGAGCGGGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGTTTTTTTTTTGACCTTAAAAACATAACCg < 2:982908/100‑1 (MQ=255) cTTGGACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGTTTTTTTTTTGACCTTAAAAACATAACCgagg < 1:374584/99‑1 (MQ=255) ggACGAGGATAGGCCTAGAGAGCGGGCCTTAACGAACAAAGACAACCGCACCGAAAGGACCGGGTTTTTTTTTTGACCTTAAAAACATAACCGAGGAGc < 1:267486/99‑1 (MQ=255) ggACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCC‑GGGTTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAg > 1:511774/1‑100 (MQ=255) gagggtgAAAGCCTTAGAGATCAAGCCTTATCGGACTAAGACACACACACAGAAAGGTCCGGGTTTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAg < 2:304944/94‑1 (MQ=255) gACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGTTTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAg > 1:1434390/1‑100 (MQ=255) gACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGTTTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAg > 1:859752/1‑100 (MQ=255) gACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGTTTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAg > 1:859840/1‑100 (MQ=255) ggAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGTTTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAAt > 2:164908/1‑100 (MQ=255) cTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGTTTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATaa > 2:1315835/1‑99 (MQ=255) ttAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGTTTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGAAAATGAATAACa > 1:319637/1‑100 (MQ=255) gCCGTAATGAAGTACGCCCCCCGCTCCGAAAGGGCCGGGTTTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAACAGCACaa < 2:1222169/95‑1 (MQ=255) ttAACGAGCTAAGACCCCCACACCGAAAGGGCCGGGTTTTTTTTTGGACCATAAAAACATAACCGAGGAGCAGACAATGAATAACAGCACAAAATTCTg < 2:732889/99‑1 (MQ=255) tAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGTTTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAACAGCACAAAATTCTGtt > 2:1041317/1‑100 (MQ=255) naCGAACTAAGACCCCCGCACCGAAAGGTCCGGGTTTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAACAGCACAAAATTCTGttt > 1:690598/2‑100 (MQ=255) cGAACTAAGACCCCCGCACCGAAAGGTCCGGGTTTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAACAGCACAAAATTCTGTTtct > 1:963963/1‑100 (MQ=255) aagtaggACCCCCGCACCGAAAGG‑GCGGGGTTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAACAGCACAAAATTCTGTTTCTCa < 2:791734/93‑1 (MQ=255) aaGACCCCCGCACCGAAAGGTCCGGGTTTTTTTTTTTTTCTTAAAAACATAACCGAGGAGCAGACAATGAATAACAGCACAAAATTCTGTTTCTCAAGAt > 2:1571933/1‑100 (MQ=255) aGACCCCCGCACCGAAAGGGGC‑GGGTTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAACAGCACAAAATTCTGTTTCTCAAGATTc < 1:935928/100‑1 (MQ=255) gACCCCCGCACCGAAAGGTCCGGGTTTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAACAGCACAAAATTCTGTTTCTCAAGATTc > 1:572980/1‑100 (MQ=255) cccccGCCCCGAAAGGTCCGGGTTTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAACAGCACAAAATTCTGTTTCTCAAGATTCa < 2:788974/99‑1 (MQ=255) cccccGCACCGAAAGGTCCGGGTTTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAACAGCACAAAATTCTGTTTCTCAAGATTCAg > 1:851194/1‑100 (MQ=255) ccccGCTCCGAAAGGTCCGGGTTTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAACAGCACAAAATTCTGTTTCTCAAGATTCAgg < 2:572977/100‑1 (MQ=255) ccGCACCGAAAGGTCCGGGTTTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAACAGCACAAAATTCTGTTTCTCAAGATTCAGGAc > 1:666593/1‑100 (MQ=255) cACCGAAAGGTCCGGGGGTTTTTTTTGACCTTAAAAACATAGCCGAGGAGCAGACAATGAAAAACAGCACAAAATTCTGTTTCTCAAGATTCAGGACggg > 2:857725/1‑100 (MQ=255) ccGAAAGGTCCGGGTTTTTTTTTTTAACTTAAAAAAATAACCACGGACCAGACAATCAATAACAGCACCAAAATCCTTTCCTCAAGCTTCAGGAc > 2:1431574/1‑95 (MQ=255) cagAAGGGTCAGGGTCTGTTTGTTGGCCGTAAAAACATAAGCGAGGAGCAGACAATCAATAACAGCACAAAATTCTGTTTCTCAAGATTc < 2:297118/88‑1 (MQ=255) gAAAGGTCC‑GGGTTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAACAGCACAAAATTCTGTTTCTCAAGATTCAGGACGGGGaact < 2:1485186/100‑1 (MQ=255) gTCCGGGTTTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAACAGCACAAAATTCTGTTTCTCAAGATTCAGGACGGGGaactaact > 1:1118311/1‑100 (MQ=255) || TTATCCCACCGTGCGGGGCTGCACTTGGACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGGTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAACAGCACAAAATTCTGTTTCTCAAGATTCAGGACGGGGAACTAACT > NC_000913/3950377‑3950559 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |