Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,525,135 (A)6→5 100% coding (9/765 nt) yfeN → putative outer membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,525,1300A.97.9% 425.8 / 0.4 95coding (4/765 nt)yfeNputative outer membrane protein
Reads supporting (aligned to +/- strand):  ref base A (1/0);  new base . (39/54);  total (40/55)
Fisher's exact test for biased strand distribution p-value = 4.26e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.85e-01

Only 100 of 107 total aligned reads displayed.

ATATTAATTCCTTTGCATTAACAATATGTCGTTTTTAGCAAACCACTTTATTAAGCAATCTACTAATAACGATCGGCATAGGGAATTATGCTATGAAAAAACATCTTTTAACTCTGACACTTTCCTCTATATTAGCGATACCTGTTGTATCTCATGCAGAATTTAAAGGCGGTTTTGCAGATATCGGCGT  >  NC_000913/2525035‑2525224
                                                                                               |                                                                                              
atatTAATTCCTTTGCATTAACAATATGTCGTTTTTAGCAAACCACTTTATTAAGCAATCTACTAATAACGATCGGCATAGGGAATTATGCTATGaaaaa                                                                                            <  2:2622653/100‑1 (MQ=255)
 tatTAATTCCTTTGCATTAACAATATGTCGTTTTTAGCAAACCACTTTATTAAGCAATCTACTAATAACGATTTTCATAGGGAATTATGCTATGaaaaac                                                                                           <  2:1468066/100‑2 (MQ=255)
 tatTAATTCCTTTGCATTAACAATATGTCGTTTTTAGCAAACCACTTTATTAAGCAATCTACTAATAACGATCGGCATAGGGAATTATGCTATGaaaaac                                                                                           >  1:2609325/1‑99 (MQ=255)
 tatTAATTCCTTTGCATTAACAATATGTCGTTTTTAGCAAACCACTTTATTAAGCAATCTACTAATAACGATCGGCATAGGGAATTATGCTATGaaaaac                                                                                           <  1:2551240/100‑2 (MQ=255)
 tatTAATTCCTTTGCATTAACAATATGTCGTTTTTAGCAAACCACTTTATTAAGCAATCTACTAATAACGATCGGCATAGGGAATTATGCTATGaaaaac                                                                                           <  2:1487538/100‑2 (MQ=255)
  atTAATTCTTTTTCATTAATTTTATGTCTTTTTTAGCAAACCACTTTATTAAGCAATGTACTAATAACGATCGGCATAGGGAATTATGCTATGaaaaaca                                                                                          <  1:1240445/100‑3 (MQ=255)
  atTAATTCCTTTGCATTAACAATATGTCGTTTTTAGCAAACCACTTTATTAAGCAATCTACTAATAACGATCGGCATAGGGAATTATGCTATGaaaaaca                                                                                          <  1:2509601/100‑3 (MQ=255)
  atTAATTCCTTTGCATTAACAATATGTCGTTTTTAGCAAACCACTTTATTAAGCAATCTACTAATAACGATCGGCATAGGGAATTATGCTATGaaaaaca                                                                                          <  1:482911/100‑3 (MQ=255)
  atTAATTCCTTTGCATTAACAATATGTCGTTTTTAGCAAACCACTTTATTAAGCAATCTACTAATAACGATCGGCATAGGGAATTATGCTATGaaaaaca                                                                                          >  2:2664810/1‑98 (MQ=255)
   ttAATTCCTTTGCATTAACAATATGTCGTTTTTAGCAAACCACTTTATTAAGCAATCTACTAATAACGATCGGCATAGGGAATTATGCTATGaaaaacat                                                                                         >  2:334971/1‑97 (MQ=255)
    tAATTCCTTTGCATTAACAATATGTCGTTTTTAGCAAACCACTTTATTAAGCAATCTACTAATAACGATCGGCATAGGGAATTATGCTATGaaaaacatc                                                                                        >  1:3030229/1‑96 (MQ=255)
    tAATTCCTTTGCATTAACAATATGTCGTTTTTAACAAACCACTTTATTAAGCAATCTACTAATAACGATCGGCATAGGGAATTATGCTATGaaaaacatc                                                                                        >  2:545225/1‑96 (MQ=255)
     aaTTCCTTTGCATTAACAATATGTTGTTTTTAGCAAACCGCTTTATTAAGCAATCGACAAATAACGATCGGCATAGGGAATTATGCTATGAAAAACATCt                                                                                      <  1:205819/100‑1 (MQ=255)
     aaTTCCTTTGCATTAACAATATGTCGTTTTTAGCAAACCACTTTATTAAGCAATCTACTAATAACGATCGGCATAGGGAATTATGCTATGAAAAACATCt                                                                                      <  1:2736688/100‑1 (MQ=255)
     aaTTCCTTTGCATTAACAATATGTCGTTTTTAGCAAACCACTTTATTAAGCAATCTACTAATAAAGATCGGAATAGGTAATTATGCTATGAAAAACATCt                                                                                      <  2:1272706/100‑1 (MQ=255)
         ccTTTGCATTAACAATATGTCGTTTTTAGCAAACCACTTTATTAAGCAATAAACTAATAACAATCAGCATAAGGAATTATGCTATGAAAAAACATCtttt                                                                                   >  1:799687/1‑100 (MQ=255)
          cTTTGCATTAACAATATGTCGTTTTTAGCAAAGCACTTTATTAAGCAATCTACTAATAACGATCGCAATAGGGAATTATGCTATGAAAAACATCTATTaa                                                                                 >  2:202763/1‑100 (MQ=255)
           tttttaTTAACAATATGTTGTTTTTAGCAAACCACTTTATTAAGCAATCTACTAATAACGATCGGCATAGGGAATTATGATATGAAAAACATCTTTTAAc                                                                                <  2:2869409/95‑1 (MQ=255)
           tttGCATTAACAATATGTCGTTTTTAGCAAACCACTTTATTAAGCAATCTACTAATAACGATCGGCATAGGGAATTATGCTATGAAAAACATCTTTTAAc                                                                                <  2:2693475/100‑1 (MQ=255)
           tttGCATTAACAATATGTCGTTTTTAGCAAACCACTTTATTAAGCAATCTACTAATAACGATCGGCATAGGGAATTATGAAATGAAAAACATCTTTTAAc                                                                                <  2:1195365/100‑1 (MQ=255)
             tGCATTAACAATATGTCGTTTTTAGCAAACCACTTTATTAAGCAATCTACTAATAACGATCGGCATAGGGAATTATGCTATGAAAAACATCTTTTAAc                                                                                <  2:2934997/98‑1 (MQ=255)
              gCATTAACAATATGTCGTTTTTAGCAAACCACTTTATTAAGCAATCTACTAATAACGATCGGCATAGGGAATTATGCTATGAAAAACATCTTTTAActct                                                                             >  1:1896794/1‑100 (MQ=255)
              gCATTAACAATATGTCGTTTTTAGCAAACCACTTTATTAAGCAATCTACTAATAACGATCGGCATAGGGAATTATGCTATGAAAAACATCTTTTAActct                                                                             <  1:2355205/100‑1 (MQ=255)
               cATTAACAATATGTCGTTTTTAGCAAAACACTTTATTAAGCAATCTACTAATAACGATCGGCATAGGGAATTATGCTATGAAAAACATCTTTTAACTCTg                                                                            <  2:2718923/100‑1 (MQ=255)
                        tgtGTCGTTTTTAGCAAACCACTTTATTAAGCAATCTACTAATAACGATCGGCATAGGGAATTATGCTATGAAAAACATCTTTTAACTCTGACACTTTcc                                                                   <  2:2836194/98‑1 (MQ=255)
                        tatGTCGTTTTTAGCAAACCACTTTATTAAGCAATTTACTAATAACGATCGGCATAGGGAATTATGCTATGAAAAACATCTTTTAACTCTGACACTTTcc                                                                   <  1:307466/100‑1 (MQ=255)
                        tatGTCGTTTTTAGCAAACCACTTTATTAAGCAATCTACTAATAACGATCGGCATAGGGAATTATGCTATGAAAAACATCTTTTAACTCTGACACttt                                                                     <  1:1895773/98‑1 (MQ=255)
                          tGTCGTTTTTAGCAAACCACTTTATTAAGCAATCTACTAATAACGATCGGCATAGGGAATTATGCTATGAAAAACATCTTTTAACTCTGACACTTTCctc                                                                 <  1:1065561/100‑1 (MQ=255)
                          tGTCGTTTTTAGCAAACCACTTTATTAAGCAATCTACTAATAACGATCGGCATAGGGAATTATGCTATGAAAAACATCTTTTAACTCTGACACTTTCctc                                                                 <  2:2742311/100‑1 (MQ=255)
                           gTCGTTTTTAGCAAACCACTTTATTAAGCAATCTACTAATAACGATCGGCATAGGGAATTATGCTATGAAAAACATCTTTTAACTCTGACACTTTCctcg                                                                <  2:1472474/100‑2 (MQ=255)
                            tCGTTTTTAGCAAACCACTTTATTAAGCAATCTACTAATAACGATCGGCATAGGGAATTATGCTATGAAAAACATCTTTTAACTCTGACACTTTCCTCta                                                               <  1:1260623/100‑1 (MQ=255)
                             cGTTTTTAGCAAACCACTTTATTAAGCAATCTACTAATAACGATCGGCATAGGGAATTATGCTATGAAAAACATCTTTTAACTCTGACACTTTCCTCtat                                                              >  2:1483591/1‑100 (MQ=255)
                             cGTTTTTAGCAAACCACTTTATTAAGCAATCTACTAATAACGATCGGCATAGGGAATTATGCTATGAAAAACATCTTTTAACTCTGACACTTTCCTCtat                                                              <  2:2033397/100‑1 (MQ=255)
                             cGTTTTTAGCAAACCACTTTATTAAGCAATCTACTAATAACGATCGGCATAGGGAATTATGCTATGAAAAACATCTTTTAACTCTGACACTTTCCTCta                                                               >  1:646005/1‑99 (MQ=255)
                              gTTTTTAGCAAACCACTTTATTAAGCAATCTACTAATAACGATCGGCATAGGGAATTATGCTATGAAAAACATCTTTTAACTCTGACACTTTCCTCtata                                                             <  1:1170249/100‑1 (MQ=255)
                              gTTTTTAGCAAACCACTTTATTAAGCAATCTACTAATAACGATCGGCATAGGGAATTATGCTATGAAAAACATCTTTTAACTCTGACACTTTCCTCtata                                                             >  1:2235833/1‑100 (MQ=255)
                                ttttAGCAAACCACTTTATTAAGCAATCTACTAATAACGATCGGCATAGGGAATTATGCTATGAAAAACATCTTTTAACTCTGACACTTTCCTCTATAtt                                                           <  2:2235810/100‑1 (MQ=255)
                                 tttAGCAAACCACTTTATTAAGCAATCTACTAATAACGATCGGCATACGAAATTATGCTATGAAAAACATCATTAAACTCTGACACTTTCCTCTATATTa                                                          >  1:2540263/1‑100 (MQ=255)
                                   tAGCAAACCACTTTATTAAGCAATCTACTAATAACGATCGGCATAGGGAATTATGCTATGAAAAACATCTTTTAACTCTGACACTTTCCTCTATATTAGc                                                        >  2:1669089/1‑100 (MQ=255)
                                     gCAAACCACTTTATTAAGAAATCGACTAATAACGATCGGCATAGGGAATTATGCTATGAAAAACATCTTTTAACTCTGACACTTTCCTCTATATTAGCGa                                                      >  2:815973/1‑100 (MQ=255)
                                       aaaCCACTTTATTAAGCAATCTACTAATAACGATCGGCATAGGGAATTATGCTATGAAAAACATCTTTTAACTCTGACACTTTCCTCTATATTAGCGATa                                                    >  1:668661/1‑100 (MQ=255)
                                       aaaCCACTTTATTAAGCAATCTACTAATAACGATCGGCATAGGGAATTATGCTATGAAAAACATATTTTAACTCTGACACTTTCCTCTATATTAAAGATa                                                    >  1:1765052/1‑100 (MQ=255)
                                         aCCACTTTATTAAGCAATCTACTAATAACGATCGGCATAGGGAATTATGCTATGAAAAACATCTTTTAACTCTGACACTTTCCTCTATATTAGCGATAcc                                                  <  1:513369/100‑1 (MQ=255)
                                          ccACTTTATTAAGCAATCTACTAATAACGATCGGCATAGGGAATTATGCTATGAAAAACATCTTTTAACTCTGACACTTTCCTCTATATTAGCGATAcc                                                  <  2:360438/99‑1 (MQ=255)
                                          ccACTTTATTAAGCAATCTACTAATAACGATCGGCATAGGGAATTATGCTATGAAAAACATCTTTTAACTCTGACACTTTCCTCTATATTAGCGATACCt                                                 >  1:2479215/1‑100 (MQ=255)
                                            aCTTTATTAAGCAATCTACTAATAACGATCGGCATAGGGAATTATGCTATGAAAAACATCTTTTAACTCTGACACTTTCCTCTATATTAGCGATACCtgt                                               <  1:1259815/100‑1 (MQ=255)
                                            aCTTTATTAAGCAATCTACTAATAACGATCGGCATAGAGAATTATGCTATGAAAAACATCTTTTAACTCTGACACTTTCCTCTATATTAGCGATACCtg                                                >  2:2302606/1‑99 (MQ=255)
                                             cTTTATTAAGCAATCTACTAATAACGATCGGCATAGGGAATTATGCTATGAAAAACATCTTTTAACTCTGACACTTTCCTCTATATTAGCGATACCtgtt                                              <  1:896418/100‑1 (MQ=255)
                                             cTTTATTAAGCAATCTACTAATAAAGATCGGAATAGGGAATTATGCTATGAAAAACATCTTTTAACTCTGACACTTTCCTCTATATAAGCTATACCtgtt                                              >  2:2689061/1‑100 (MQ=255)
                                              tttATTAAGCAATCTACTAATAACGATCGGCATAGGGAATTATGCTATGAAAAACATCTTTTAACTCTGACACTTTCCTCTATATTAGCGATACCtgttg                                             <  2:2939809/100‑1 (MQ=255)
                                               ttattaAGCAATCTACTAATAACGATCGGCATAGGGAATTATGCTATGAAAAACATCTTTTAACTCTGACACTTTCCTCTATATTAGCGATACCtgttgt                                            >  2:655124/1‑100 (MQ=255)
                                               ttattaAGCAATCTACTAATAACGATCGGCATAGGGAATTATGCTATGAAAAACATCTTTTAACTCTGACACTTTCCTCTATATTAGCGATACCtgttgt                                            >  2:1335493/1‑100 (MQ=255)
                                                tattaAGCAATCTACTAATAACGATCGGCATAGGGAATTATGCTATGAAAAACATCTTTTAACTCTGACACTTTCCTCTATATTAGCGATACCTGTTGTa                                           <  1:190939/100‑1 (MQ=255)
                                                tattaAGCAATCTACTAATAACGATCGGCATAGGGAATTATGCTATGAAAAACATCTTTTAACTCTGACACTTTCCTCTATATTAGCGATACCTGTTGTa                                           >  1:34530/1‑100 (MQ=255)
                                                    aaGCAATCTACTAATAACGATCGGCATAGGGAATTATGCTATGAAAAACATCTTTTAACTCTGACACTTTCCTCTATATTAGCGATACCTGTTGTAtctc                                       <  2:2259783/100‑1 (MQ=255)
                                                    aaGCAATCTACTAATAACGATCGGCATAGGGAATTATGCTATGAAAAACATCTTTTAACTCTGACACTTTCCTCTATATTAGCGATACCTGTTGTAtctc                                       >  1:3043956/1‑100 (MQ=255)
                                                     aGCAATCTACTAATAACGATCGGCATAGGGAATTATGCTATGAAAAACATCTTTTAACTCTGACACTTTCCTCTATATTAGCGATACCTGTTGTATCTCa                                      <  1:2691845/100‑1 (MQ=255)
                                                      gCAATCTACTAATAACGATCGGCATAGGGAATTATGCTATGAAAAACATCTTTTAACTCTGACACTTTCCTCTATATTAGCGATACCTGTTGTATCTCAt                                     <  2:413943/100‑1 (MQ=255)
                                                        aaTCTACTAATAACGATCGGCATAGGGAATTATGCTATGAAAAACATCTTTTAACTCTGACACTTTCCTCTATATTAGCGATACCTGTTGTATCTCATGc                                   <  2:2486541/100‑1 (MQ=255)
                                                        aaTCTACTAATAACGATCGGCATAGGGAATTATGCTATGAAAAACATCTTTTAACTCTGACACTTTCCTCTATATTAGCGATACCTGTTGTATCTCATGc                                   <  1:2624185/100‑1 (MQ=255)
                                                        aaTCTACTAATAACGATCGGCATAGGGAATTATGCTATTAAAAACATCTTTTAACTCTGACACTTTCCTCTATATTAGCGATACCTGTTGTATCTCATGc                                   <  1:142449/100‑1 (MQ=255)
                                                          tCTACTAATAACGATCGGCATAGGGAATTATGCTATGAAAAACATCTTTTAACTCTGACACTTTCCTCTATATTAGCGATACCTGTTGTATCTCAt                                     <  1:665926/96‑1 (MQ=255)
                                                          tCTACTAATAACGATCGGCATAGGGAATTATGCTATGAAAAACATCTTTTAACTCTGACACTTTCCTCTATATTAGCGATACCTGTTGTATCTCATGCAg                                 <  2:2632418/100‑1 (MQ=255)
                                                           ctactaATAACGATCGGCATAGGGAATTATGCTATGAAAAACATCTTTTAACTCTGACACTTTCCTCTATATTAGCGATACCTGTTGTATCTCATGCAGa                                <  2:1782102/100‑1 (MQ=255)
                                                            tactaATAACGATCGGCATAGGGAATTATGCTATGAAAAACATCTTTTAACTCTGACACTTTCCTCTATATTAGCGATACCTGTTGTATCTCATGCAGaa                               >  2:1811899/1‑100 (MQ=255)
                                                             actaATAACGATCGGCATAGGGAATTATGCTATGAAAAACATCTTTTAACTCTGACACTTTCCTCTATATTAGCGATACCTGTTGTATCTCATGCAGAAt                              >  1:2466597/1‑100 (MQ=255)
                                                             actaATAACGATCGGCATAGGGAATTATGCTATGAAAAACATCTTTTAACTATGACAATTTCCTCTATATTAGCGATAAATGTTGTAACTCATCCAGAAt                              >  2:3044767/1‑100 (MQ=255)
                                                               taataaCGATCGGCATAGGGAATTATGCTATGAAAAACATCTTTTAACTCTGACACTTTCCTCTATATTAGCGATACCTGTTGTATCTCATGCAGAAttt                            <  1:1483607/100‑1 (MQ=255)
                                                               taataaCGATCGGCATAGGGAATTATGCTATGAAAAACATCTTTTAACTCTGACACTTTCCTCTATATTAGCGATACCTGTTGTATCTCATGCAGAAttt                            <  1:1399264/100‑1 (MQ=255)
                                                               taataaCGATCGGCATAGGGAATTATGCTATGAAAAACATCTTTTAACTCTGACACTTTCCTCTATATTAGCGATACCTGTTGTATCTCATGCAGAAttt                            <  2:1227800/100‑1 (MQ=255)
                                                                aataaCGATCGGCATAGGGAATTATGCTATGAAAAACATCTTTTAACTCTGACACTTTCCTCTATATTAGCGATACCTGTTGTATCTCATGCAGAATTTa                           >  2:1399251/1‑100 (MQ=255)
                                                                aataaCGATCGGCATAGGGAATTATGCTATGAAAAACATCTTTTAACTCTGACACTTTCCTCTATATTAGCGATACCTGTTGTATCTCATGCAGAATTTa                           >  2:2761857/1‑100 (MQ=255)
                                                                  taaCGATCGGCATAGGGAATTATGCTATGAAAAACATCTTTTAACTCTGACACTTTCCTCTATATTAGCGATACCTGTTGTATCTCATGCAGAATTTaaa                         >  2:1438139/1‑100 (MQ=255)
                                                                  taaCGATCGGCATAGGGAATTATGCTATGAAAAACATCTTTTAACTCTGACACTTTCCTCTATATTAGCGATACCTGTTGTATCTCATGCAGAATTTaaa                         <  1:225292/100‑1 (MQ=255)
                                                                   aaCGATCGGCATAGGGAATTATGCTATGAAAAACATCTTTTAACTCTGACACTTTCCTCTATATTAGCGATACCTGTTGTATCTCATGCAGAATTTAAAg                        <  2:2898626/100‑1 (MQ=255)
                                                                   aaCGATCGGCATAGGGAATTATGCTATGAAAAACATCTTTTAACTCTGACACTTTCCTCTATATTAGCGATACCTGTTGTATCTCATGCAGAATTTAAAg                        >  2:2488708/1‑100 (MQ=255)
                                                                    aCGATCGGCATAGGGAATTATGCTATGAAAAACATCTTTTAACTCTGACACTTTCCTCTATATTAGCGATACCTGTTGTATCTCATGCAGAATTTAAAgg                       <  2:1236641/100‑1 (MQ=255)
                                                                    aCGATCGGCATAGGGAATTATGCTATGAAAAACATCTTTTAACTCTGACACTTTCCTCTATATTAGCGATACCTGTTGTATCTCATGCAGAATTTAAAgg                       >  1:909222/1‑100 (MQ=255)
                                                                     cGATCGGCATAGGGAATTATGCTATGAAAAACATCTTTTAACTCTGACACTTTCCTCTATATTAGCGATACTTGTTGTATCTCATGCAGAATTTAAAGGc                      >  2:2483121/1‑100 (MQ=255)
                                                                     cGATCGGCATAGGGAATTATGCTATGAAAAACATCTTTTAACTCTGACACTTTCCTCTATATTAGCGATACCTGTTGTATCTCATGCAGAATTTAAAgg                       >  1:2456178/1‑99 (MQ=255)
                                                                     cGATCGGCATAGGGAATTATGCTATGAAAAACATCTTTTAACTCTGACACTTTCCTCTATATTAGCGATACCTGTTGAATCTCATGCAGAATTTaaaag                       >  1:2471806/1‑97 (MQ=255)
                                                                      gATCGGCATAGGGAATTATGCTATGAAAAACATCTTTTAACTCTGACACTTTCCTCTATATTAGCGATACCTGTTGTATCTCATGCAGAATTTAAAGGCg                     >  2:2061828/1‑100 (MQ=255)
                                                                       aTCGGCATAGGGAATTATGCTATGAAAAACATCTTTTAACTCTGACACTTTCCTCTATATTAGCGATACCTGTTGTATCTCATGCAGAATTTAAAGGCgg                    >  1:2811999/1‑100 (MQ=255)
                                                                        tCGGCATAGGGAATTATGCTATGAAAAACATCTTTTAACTCTGACACTTTCCTCTATATTAACGATACCTGTTGTATCTCATGCAGCATTTAAAGGCGGt                   <  2:1868091/100‑1 (MQ=255)
                                                                          ggCATAGGGAATTATGCTATGAAAAACATCTTTTAACTCTGACACTTTCCTCTATATTAGCGATACCTGTTGTATCTCATGCAGAATTTAAAGGCGGttt                 >  2:1177005/1‑100 (MQ=255)
                                                                          ggCATAGGGAATTATGCTATGAAAAACATCTTTTAACTCTGACACTTTCCTCTATATTAGCGATACCTGTTGTATCTCATGCAGAATTTAAAGGCGGttt                 >  2:1582310/1‑100 (MQ=255)
                                                                          ggCATAGGGAATTATGCTATGAAAAACATCTTTTAACTCTGACACTTTCCTCTATATTAGCGATACCTGTTGTATCTAATGCAGAATTTAAAGGCGGtt                  >  1:548155/1‑99 (MQ=255)
                                                                           gCATAGGGAATTATGCTATGAAAAACATCTTTTAACTCTGACACCTTCCTCTATATTAGCGATACCTGTTGTATCTCATGCAGAATTTAAAGGCGGtttt                >  1:434014/1‑100 (MQ=255)
                                                                             aTAGGGAATTATGCTATGAAAAACATCTTTTAACTCTGACACTTTCCTCTATATTAGCGATACCTGTTGTATCTCATGCAGAATTTAAAGGCGGTTTTGc              <  2:909216/100‑1 (MQ=255)
                                                                             aTAGGGAATTATGCTATGAAAAACATCTTTTAACTCTGACACTTTCCTCTATATTAGCGATACCTGTTGTATCTCATGCAGAATTTAAAGGCGGTTTTGc              >  2:254214/1‑100 (MQ=255)
                                                                              tAGGGAATTATGTTATGAAAAACATCTTTTAACTCTGACACTTTCCTCTATATTAGCGATACCTGTTGTATCTCATGCAGAATTTAAAGGCGGTTTTGCa             <  1:2065881/100‑1 (MQ=255)
                                                                              tAGGGAATTATGCTATGAAAAACATCTTTTAACTCTGACACTTTCCTCTATATTAGCGATACCTGTTGTATCTCATGCAGAATTTAAAGGCGGTTTTGCa             <  2:1738406/100‑1 (MQ=255)
                                                                              tAGGGAAGTATTCTATGAAAAACATCTTTTAACACTTACCCTTTCCTTTATATTAGCGATGCCTGTTGTATCTCATGCAGAATTTAAAGGAGGTTTTGc              <  2:1013322/99‑1 (MQ=255)
                                                                              tAGCGAATTATGCTATGAAAAACATCTTTTAACTCTGACACTTTCCTCTATATTAGCTATACCTGTTGTATCTCATGCAGAATTTAAAGGCGGTTTTGCa             <  2:1362631/100‑1 (MQ=255)
                                                                                gggAATTATGCTATGAAAAACATCTTTTAACTCTTACACTTTCCTCTATATTAGCGATACCTGTTTTATCTCATGCATAATTTAAAAGCGGTTTTGCAg            >  1:2905159/1‑99 (MQ=255)
                                                                                gggAATTATGCTATGAAAAACATCTTTTAACTCTGACACTTTCCTCTATATTAGCGATACCTGTTGTATCTCATGCAGAATTTAAAGGCGGTTTTGCAg            <  1:1389136/99‑1 (MQ=255)
                                                                                gggAATTATGCTATGAAAAACATCTTTTAACTCTGACACTTTCCTCTATATTAGCGATACCTGTTGTATCTCATGCAGAATTTAAAGGCGGTTTTGCAGa           <  1:2676620/100‑1 (MQ=255)
                                                                                  gAATTATGCTATGAAAAACATCTTTTAACTCTGACACTTTCCTCTATATTAGCGATACCTGTTGTATCTCATGCAGAATTTAAAGGCGGTTTTGCAGata         >  1:2318334/1‑100 (MQ=255)
                                                                                       aTGCTATGAAAAACATCTTTTAACTCTTACACTTGCCTCTATATTAGCGATACCCGTTGTCTTTCATGCATAATTTAAAGGCGGTTTTGCAGATATCTGc    >  2:1499938/1‑100 (MQ=255)
                                                                                         gCTATGAAAAACATCTTTTAACTCTGACACTTTCCTCTATATTAGCGATACCTGTTGTATCTCATGCAGAATTTAAAGGCGGTTTTGCAGATATCGGCGt  <  1:1847309/100‑1 (MQ=255)
                                                                                               |                                                                                              
ATATTAATTCCTTTGCATTAACAATATGTCGTTTTTAGCAAACCACTTTATTAAGCAATCTACTAATAACGATCGGCATAGGGAATTATGCTATGAAAAAACATCTTTTAACTCTGACACTTTCCTCTATATTAGCGATACCTGTTGTATCTCATGCAGAATTTAAAGGCGGTTTTGCAGATATCGGCGT  >  NC_000913/2525035‑2525224

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: