Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,927,735 Δ1 bp 100% coding (581/993 nt) asnA → asparagine synthetase A

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,927,7340G.97.4% 508.0 / 4.7 113coding (580/993 nt)asnAasparagine synthetase A
Reads supporting (aligned to +/- strand):  ref base G (2/0);  new base . (61/49);  total (63/50)
Fisher's exact test for biased strand distribution p-value = 5.03e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.00e-01

Only 100 of 123 total aligned reads displayed.

CTGCCGGATCAGATCCACTTCGTACACAGCCAGGAGTTACTGTCTCGTTATCCGGATCTTGATGCCAAAGGGCGTGAGCGGGCGATAGCGAAAGATCTTGGCGCGGTATTCCTTGTCGGGATTGGCGGCAAGCTGAGCGATGGTCATCGCCACGACGTGCGCGCACCGGATTATGATGACTGGAGCACCCCGTCAGAG  >  NC_000913/3927635‑3927832
                                                                                                   |                                                                                                  
cTGCCGGATCAGATCCACTTCGTACACAGCCAGGAGTTACTGTCTCGTTATCCGGATATTGATGCCAAAGGGCGTGAGCGGGCGATAGCGAAAGATCTTg                                                                                                    >  1:816958/1‑100 (MQ=255)
 tGCCGGATCAGATCCACTTCGTACACAGCCATGAGTTACTGTCTCGTTATCCGGATCTTGATGCCAAAGGGCGTGAGCGGGCGATAGCGAAAGATCTTgn                                                                                                   <  1:1436051/100‑2 (MQ=255)
  gCCGGATCAGATCCACTTCGTACACAGCCAGGAGTTACTGTCTCGTTATCCTGATCTTGATGCCAAAGGGCGTGAGCGGGCGATAGCGCAAGGTCTTgcg                                                                                                  <  2:1585587/100‑3 (MQ=255)
  gCCGGATCAGATCCACTTCGTACACAGCCAGGAGTTACTGTCTCGTTATCCGGATCTTGATGCCAAAGGGCGTGAGCGGGCGATAGCGAAAGATCTTgcg                                                                                                  <  2:684100/100‑3 (MQ=255)
   ccGGATCAGATCCACTTCGTACACAGCCAGGAGTTACTGTCTCGTTATCCGGATCTTGATGCCAAAGGGCGTGAGCGGGCGATAGCGAAAGATCTTgcgc                                                                                                 <  1:2366826/100‑4 (MQ=255)
    cGGATCAGATCCACTTCGTACACAGCCAGGAGTTACTGTCTCGTTATCCGGATCTTGATGCCAAAGGGCGTGAGCGGGCGATAGCGAAAGATCTTgcgcg                                                                                                >  1:1280981/1‑96 (MQ=255)
     ggATCAGATCCACTTCGTACACAGCCAGGAGTTACTGTCTCGTTATCCGGATCTTGATGCCAAAGGGCGTGAGCGGGCGATAGCGAAAGATCTT‑GCGCgg                                                                                              >  1:755362/1‑100 (MQ=255)
      gATCAGATCCACTTCGTACACAGCCAGGAGTTACTGTCTCGTTATCCGGATCTTGATGCCAAAGGGCGTGAGCGGGCGATAGCGAAAGATCTT‑GCGCGGt                                                                                             <  1:418854/100‑1 (MQ=255)
        tCAGATCCACTTCGTACACAGCCAGGAGTTACTGTCTCGTTATCCGGATCTTGATGCCAAAGGGCGTGAGCGGGCGATAGCGAAAGATCTT‑GCGCGGTAt                                                                                           <  1:2309690/100‑1 (MQ=255)
          aGATCCACTTCGTACACAGCCAGGAGTTACTGTCTCGTTATCCGGATCTTGATGCCAAAGGGCGTGAGCGGGCGATAGCGAAAGATCTT‑GCGCGGTATTc                                                                                         >  1:1055289/1‑100 (MQ=255)
          aGATCCACTTCGTACACAGCCAGGAGTTACTGTCTCGTTATCCGGATCTTGATGCCAAAGGGCGTGAGCGGGCGATAGCGAAAGATCTT‑GCGCGGTATTc                                                                                         >  1:1148524/1‑100 (MQ=255)
          aGATCCACTTCGTACACAGCCAGGAGTTACTGTCTCGTTATCCGGATCTTGATGCCAAAGGGCGTGAGCGGGCGATAGCGAAAGATCTT‑GCGCGGTATTc                                                                                         >  1:2295756/1‑100 (MQ=255)
           gATCCACTTCGTACACAGCCAGGAGTTACTGTCTCGTTATCCGGATCTTGATGCCAAAGGGCGTGAGCGGGCGATAGCGAAAGATCTT‑ACGCGGTATTcc                                                                                        >  1:255713/1‑100 (MQ=255)
             tCCACTTCGTACACAGCCAGGAGTTACTGTCTCGTTATCCGGATCTTGATGCCAAAGGGCGTGAGCGGGCGATAGCGAAAGATCTT‑GCGCGGTATTCCtt                                                                                      <  1:1457042/100‑1 (MQ=255)
               cACTTCGTACACAGCCAGGAGTTACTGTCTCGTTATCCGGATCTTGATGCCAAAGGGCGTGAGCGGGCGATAGCGAAAGATCTT‑GCGCGGTATTCCTTGt                                                                                    >  1:298078/1‑100 (MQ=255)
                aCTTCGTACACAGCCAGGAGTTACTGTCTCGTTATCCGGATCTTGATGCCAAAGGGCGTGAGCGGGCGATAGCGAAAGATCTT‑GCGCGGTATTCATTGTc                                                                                   >  1:2455942/1‑100 (MQ=255)
                 cTTCGTACACAGCCAGGAGTTACTGTCTCGTTATCCGTATCTTGATGCCAAAGGGCGTGAGCGGGCGATAGCGAAAGATCTT‑GCGCGGTATTCCTTGTCg                                                                                  <  2:957005/100‑1 (MQ=255)
                 cTTCGTACACAGCCAGGAGTTACTGTCTCGTTATCCGGATATTGATGCCAAAGGGCGTGAGCGGGCGATAGCGAAAGATCTT‑GCGCGGTATTCCTTGTCg                                                                                  <  1:1638074/100‑1 (MQ=255)
                  ttCGTACACAGCCAGGAGTTACTGTCTCGTTATCCGGATCTTGATGCCAAAGGGCGTGAGCGGGCGATAGCGAAAGATCTT‑GCGCGGTATTCCTTGTCgg                                                                                 <  2:2753971/100‑1 (MQ=255)
                     gTACACAGCCAGGAGTTACTGTCTCGTTATCCGGATCTTGATGCCAAAGGGCGTGAGCGGGCGATAGCGAAAGATCTT‑GCGCGGTATTCCTTGTCGGGAt                                                                              <  2:1631831/100‑1 (MQ=255)
                       acacAGCCAGGAGTTACTGTCTCGTTATCCGGATCTTGATGCCAAAGGGCGTGAGCGGGCGATAGCGAAAGATCTT‑GCGCGGTATTCCTTGTCGGGATTg                                                                            >  2:1706107/1‑100 (MQ=255)
                       acacAGCCAGGAGTTACTGTCTCGTTATCCGGATCTTGATGCCAAAGGGCGTGAGCGGGCGATAGCGAAAGATCTT‑GCGCGGTATTCCTTGTCGGGATTg                                                                            >  2:2742398/1‑100 (MQ=255)
                         acaGCCAGGAGTTACTGTCTCGTTATCCGTATCTTGATGCCAAAAGGCGTCAACGGGCGATAGCGAAAGATGTT‑GCGCGGTATTCCTTGTCggggtagg                                                                           >  2:872445/1‑94 (MQ=255)
                         acaGCCAGGAGTTACTGTCTCGTTATCCGGATCTTGATGCCAAAGGGCGTGAGCGGGCGATAGCGAAAGATCTT‑GCGCGGTATTCCTTGTCGGGATTggc                                                                          >  2:2532950/1‑100 (MQ=255)
                          caGCCAGGAGTTACTGTCTCGTTATCCGGATCTTGATGCCAAAGGGCGTGAGCGGGCGATAGCGAAAGATCTT‑GCGCGGTATTCCTTGTCGGGATTggcg                                                                         >  1:1550761/1‑100 (MQ=255)
                          caGCCAGGAGTTACTGTCTCGTTATCCGGATCTTGATGCCAAAGGGCGTGAGCGGGCGATAGCGAAAGATCTT‑GCGCGGTATTCCTTGTCGGGATTggcg                                                                         >  2:1125762/1‑100 (MQ=255)
                          caGCCAGGAGTTACTGTCTCGTTATCCGGATCTTGATGCCAAAGGGCGTGAGCGGGCGATAGCGAAAGATCTT‑GCGCGGTATTCCTTGTCGGGATTggcg                                                                         >  2:356844/1‑100 (MQ=255)
                          caGCCAGGAGTTACTGTCTCGTTATCCGGATCTTGATGCCAAAGGGCGTGAGCGGGCGATAGCGAAAGATCTT‑GCGCGGTATTCCTTGTCGGGATTggcg                                                                         <  1:409465/100‑1 (MQ=255)
                          caGCCAGGAGTTACTGTCTCGTTATCCGGATCTTGATGCCAAAGGGCGTGAGCGGGCGATAGCGAAAGATCTT‑GCGCGGTATTCCTTGTCGGGATTggc                                                                          >  2:95625/1‑99 (MQ=255)
                             ccAGGAGTTACTGTCTCGTTATCCGGATCTTGATGCCAAAGGGCGTGAGCGGGCGATAGCGAAAGATCTTGGCGCGGTATTCCTTGTCGGGATTggcggc                                                                       >  1:558321/1‑100 (MQ=255)
                             ccAGGAGTTAATGTCTCGTTATCCGGATCTTGATGCCAAAGGGCGTGAGCGGGCGATAGCGAAAGATCTT‑GCGCGGTATTCCTTGTCGGGATTggc                                                                          <  2:1502248/96‑1 (MQ=255)
                               aGGAGTTACTGTCTCGTTATCCGGATCTTGATGCCAAAGGGCGTGAGCGGGCGATAGCGAAAGATCTT‑GCGCGGTATTCCTTGTCGGGATTGGCGGCAAg                                                                    <  1:1217065/100‑1 (MQ=255)
                                ggAGTTACTGTCTCGTTATCCGGATCTTGATGCCAAAGGGCGTGAGCGGGCGATAGCGAAAGATCTT‑GCGCGGTATTCCTTGTCGGGATTGGCGGCAAGc                                                                   >  1:698001/1‑100 (MQ=255)
                                   gTTACTGTCTCGTTATCCGGATCTTGATGCCAAAGGGCGTGAGCGGGCGATAGCGAAAGATCTT‑GCGCGGTATTCCTTGTCGGGATTGGCGGCAAGCTGa                                                                >  1:1793214/1‑100 (MQ=255)
                                    ttACTGTCTCGTTATCCGGATCTTGATGCCAAAGGGCGTGAGCGGGCGATAGCGAAAGATCTT‑GCGCGGTATTCCTTGTCGGGATTGGCGGCAAGCTGAg                                                               <  2:2610604/100‑1 (MQ=255)
                                    ttACTGTCTCGTTATCCGGATCTTGATGCCAAAGGGCGTGAGCGGGCGATAACGAAAGATCTT‑GCGCGGTATTCCTTGTCGGGATTGGCGGCAAGCTGAg                                                               <  1:1022729/100‑1 (MQ=255)
                                     tACTGTCTCGTTATCCGGATCTTGATGCCAAAGGGCGTGAGCGGGCGATAGCGAAAGATCTT‑GCGCGGTATTCCTTGTCGGGATTGGCGGCAAGCTGAGc                                                              <  2:1101966/100‑1 (MQ=255)
                                     tACTGTCTCGTTATCCGGATCTTGATGCCAAAGGGCGTGAGCGGGCGATAGCGAAAGATCTT‑GCGCGGTATTCCTTGTCGGGATTGGCGGCAAGCTGAGc                                                              <  2:66947/100‑1 (MQ=255)
                                       ntGTCTCGTTATCCGGATCTTGATGCCAAAGGGCGTGAGCGGGCGATAGCGAAAGATCTT‑GCGCGGTATTCCTTGTCGGGATTGGCGGCAAGCTGAGCGa                                                            >  2:2427415/2‑100 (MQ=255)
                                       cTGTCTCGTTATCCGGATCTTGATGCCAAAGGGCGTGAGCGGGCGATAGCGAAAGATCTT‑GCGCGGTATTCCTTGTCGGGATTGGCGGCAAGCTGAGCGa                                                            >  1:2558628/1‑100 (MQ=255)
                                       cTGTCTCGTTATCCGGATCTTGATGCCAAAGGGCGTGAGCGGGCGATAGCGAAAGATCTT‑GCGCGGTATTCCTTGTCGGGATTGGCGGCAAGCTGAGCGa                                                            >  2:2708735/1‑100 (MQ=255)
                                        tGTCTCGTTATCCGGATCTTGATGCCAAAGGGCGTGAGCGGGCGATAGCGAAAGATCTT‑GCGCGGTATTCCTTGTCGGGATTGGCGGCAAGCTGAGCGAt                                                           >  1:639195/1‑100 (MQ=255)
                                         gTCTCGTTATCCGGATCTTGATGCCAAAGGGCGTGAGCGGGCGATAGCGAAAGATCTT‑GCGCGGTATTCCTTGTCGGGATTGGCGGCAAGCTGAGCGATg                                                          >  1:1932945/1‑100 (MQ=255)
                                         gTCTCGTTATCCGGATCTTGATGCCAAAGGGCGTGAGCGGGCGATAGCGAAAGATCTT‑GCGCGGTATTCCTTGTCGGGATTGGCGGCAAGCTGAGCGATg                                                          <  2:2830428/100‑1 (MQ=255)
                                          tctcGTTATCCGGATCTTGATGCCAAAGGGCGTGAGCGGGCGATAGCGAAAGATCTT‑GCGCGGTATTCCTTGTCGGGATTGGCGGCAAGCTGAGCGATgg                                                         <  2:639191/100‑1 (MQ=255)
                                            tcGTTATCCGGATCTTGATGCCAAAGGGCGTGAGCGGGCGATAGCGAAAGATCTT‑GCGCGGTATTCCTTGTCGGGATTGGCGGCAAGCTGAGCGATGGt                                                        >  2:2155634/1‑99 (MQ=255)
                                            tcGTTATCCGGATCTTGATGCCAAAGGGCGTGAGCGGGCGATAGCGAAAGATCTT‑GCGCGGTATTCCTTGTCGGGATTGGCGGCAAGCTGAGCGATGGTc                                                       >  1:1312123/1‑100 (MQ=255)
                                             cGTTATCCGGATCTTGATGCCAAAGGGCGTGAGCGGGCGATAGCGAAAGATCTT‑GCGCGGTATTCCTTGTCGGGATTGGCGGCAAGCTGAGCGATGGTCa                                                      <  1:1321874/100‑1 (MQ=255)
                                             cGTTATCCGGATCTTGATGCCAAAGGGCGTGAGCGGGCGATAGCGAAAGATCTT‑GCGCGGTATTCCTTGTCGGGATTGGCGGCAAGCTGAGCGATGGTCa                                                      <  1:1969615/100‑1 (MQ=255)
                                               ttATCCGGATCTTGATGCCAAAGGGCGTGAGCGGGCGATAGCGAAAGATCTT‑GCGCGGTATTCCTTGTCGGGATTGGCGGCAAGCTGAGCGATGGTCATc                                                    >  1:758395/1‑100 (MQ=255)
                                                 aTCCGGATCTTGATGCCAAAGGGCGTGAGCGGGCGATAGCGAAAGATCTT‑GCGCGGTATTCCTTGTCGGGATTGGCGGCAAGCTGAGCGATGGTCATCGc                                                  >  2:2980930/1‑100 (MQ=255)
                                                   ccGGATCTTGATGCCAAAGGGCGAGAGTGGGCGATAGCGAAAGATCTT‑GCGCGGTATTCCTTGTCGGGATTGGCGGCAAGCTGAGCGATGGTCATCGCCa                                                >  2:84264/1‑100 (MQ=255)
                                                    cGGATCTTGATGCCAAAGGGCGTGAGCGGGCGATAGCGAAAGATCTT‑GCGCGGTATTCCTTGTCGTGATTGGCGGCAAGCTGAGCGATGGTCATCGCCac                                               <  2:629646/100‑1 (MQ=255)
                                                    cGGATCTTGATGCCAAAGGGCGTGAGCGGGCGATAGCGAAAGATCTT‑GCGCGGTATTACTTGTCGGGATTGGCGGAAAGCTGAGCGATGGTCATCGCCac                                               >  1:2373699/1‑100 (MQ=255)
                                                     ggATCTTGATGCCAAAGGGCGTGGGCGGGCGATAGCGAAAGATCTT‑GCGCGGTATTCCTTGTCGGGATTGGCGGCAAGCTGAGCGATGGTCATCGCCacg                                              <  1:952887/100‑1 (MQ=255)
                                                        tCTTGATGCCAAAGGGCGTGAGCGGGCGATAGCGAAAGATCTT‑GCGCGGTATTCCTTGTCGGGATTGGCGGCAAGCTGAGCGATGGTCATCGCCacgacg                                           <  2:1550743/100‑1 (MQ=255)
                                                        tCTTGATGCCAAAGGGCGTGAGCGGGCGATAGCGAAAGATCTT‑GCGCGGTATTCCTTGTCGGGATTGGCGGCAAGCTGAGCGATGGTCATCGCCacgacg                                           >  2:1891608/1‑100 (MQ=255)
                                                        tCTTGATGCCAAAGGGCGTGAGCGGGCGATAGCGAAAGATCTT‑GCGCGGTATTCCTTGTCGGGATTGGCGGCAAGCTGAGCGATGGTCATCGCCacgacg                                           >  2:1891900/1‑100 (MQ=255)
                                                         cTTTATGCTAATGGGTGTGAGCGGGCGATAGCGAAAGATCTT‑GCGCGGTATGCCTTGGCGGGATTGGCGGCAAGCTGAGCGATGGTCATCGCCacgacg                                           <  1:459333/99‑1 (MQ=255)
                                                         cTTGATGCCAAAGGGCGTGAGCGGGCGATAGCGAAAGATCTT‑GCGCGGTATTCCTTGTCGGGATTGGCGGCAAGCTGAGCGATGGTCATCGCCACGACGt                                          >  2:66354/1‑100 (MQ=255)
                                                         cTTGATGCCAAAGGGCGTGAGCGGGCGATAGCGAAAGATCTT‑GCGCGGTATTCCTTGTCGGGATTGGCGGCAAGCTGAGCGATGGTCATCGCCACGACGt                                          >  2:282292/1‑100 (MQ=255)
                                                           tGATGCCAAAGGGCGTGAGCGGGCGATAGCGAAAGATCTT‑GCGCGGTATTCCTTGTCGGGATTGGCGGCAAGCTGAGCGATGGTCATCGCCACGACGTgc                                        >  2:158590/1‑100 (MQ=255)
                                                             aTGCCAAAGGGCGTGAGCGGGCGATAGCGAAAGATCTT‑GCGCGGTATTCCTTGTCGGGATTGGCGGCAAGCTGAGCGATGGTCATCGCCACGACGTgcgc                                      >  1:460679/1‑100 (MQ=255)
                                                              tGCCAAAGGGCGTGAGCGGGCGATAGCGAAAGATCTT‑GCGCGGTATTCCTTGTCGGGATTGGCGGCAAGCTGAGCGATGGTCATCGCCACGACGTgcgcg                                     >  1:2408528/1‑100 (MQ=255)
                                                               gCCAAAGGGCGTGAGCGGGCGATAGCGAAAGATCTT‑GCGCGGTATTCCTTGTCGGGATTGGCGGCAAGCTGAGCGATGGTCATCGCCACGACGTgcgcgn                                    <  1:2427444/100‑2 (MQ=255)
                                                               gCCAAAGGGCGTGAGCGGGCGATAGCGAAAGATCTT‑GCGCGGTATTCCTTGTCGGGATTGGCGGCAAGCTGAGCGATGGTCATCGCCACGACGTgcgcgc                                    >  2:2960784/1‑100 (MQ=255)
                                                                ccAAAGGGCGTGAGCGGGCGATAGCGAAAGATCTT‑GCGCGGTATTCCTTGTCGGGATTGGCGGCAAGCTGAGCGATGGTCATCGCCACGACGTGCGCGCa                                   >  2:979882/1‑100 (MQ=255)
                                                                ccAAAGGGCGTGAGCGGGCGATAGCGAAAGATCTT‑GCGCGGTATTCCTTGTCGGGATTGGCGGCAAGCTGAGCGATGGTCATCGCCACGACGTGCGCGCa                                   >  1:2436692/1‑100 (MQ=255)
                                                                    aGGGCGTGAGCGGGCGATAGCGAAAGATCTT‑GCGCGGTATTCCTTGTCGGGATTGGCGGCAAGCTGAGCGATGGTCATCGCCACGACGTGCGCGCACCgg                               <  1:2514236/100‑1 (MQ=255)
                                                                    aGGGCGTGAGCGGGCGATAGCGAAAGATCTT‑GCGCGGTATTCCTTGTCGGGATTGGCGGCAAGCTGAGCGATGGTCATCGCCACGACGTGCGCGCACCgg                               <  2:1782795/100‑1 (MQ=255)
                                                                     gggCGTGAGCGGGCGATAGCGAAAGATCTT‑GCGCGGTATTCCTTGTCGGGATTGGCGGCAAGCTGAGCGATGGTCATCGCCACGACGTGCGCGCACCGGa                              >  2:2475809/1‑100 (MQ=255)
                                                                      ggCGTGCGCGGGCGATAGCGAAAGATCTT‑GCGCGGTATTCCTTGTCGGGATTGGCGGCAAGCTGCGCGATAGTCATCGCCAAGACGTgcgagccc                                  >  2:2801110/1‑93 (MQ=255)
                                                                      ggCGTGAGCGGGCGATAGCGAAAGATCTT‑GCGCGGTATTCCTTGTCGGGATTGGCGGCAAGCTCAGCGATGGTCATCGCCACGACGTGCGCGCACCGGAt                             <  1:2146957/100‑1 (MQ=255)
                                                                         gTGAGCGGGCGATAGCGAAAGATCTT‑GCGCGGTATTCCTTGTCGGGATTGGCGGCAAGCTGAGCGATGGTCATCGCCACGACGTGCGCGCACCGGATTat                          <  2:542610/100‑1 (MQ=255)
                                                                          tGAGCGGGCGATAGCGAAAGATCTT‑GCGCGGTATTCCTTGTCGGGATTGGCGGCAAGCTGAGCGATGGTCATCGCCACGACGTGCGCGCACCGGATTatg                         <  2:2254041/100‑1 (MQ=255)
                                                                          tGAGCGGGCGATAGCGAAAGATCTT‑GCGCGGTATTCCTTGTCGGGATTGGCGGCAAGCTGAGCGATGGTCATCGCCACGACGTGCGCGCACCGGATTatg                         <  2:370669/100‑1 (MQ=255)
                                                                           gAGCGGGCGATAGCGAAAGATCTT‑GCGCGGTATTCCTTGTCGGGATTGGCGGCAAGCTGAGCGATGGTCATCGCCACGACGTGCGCGCACCGGATTatga                        >  2:923971/1‑100 (MQ=255)
                                                                            aGCGGGCGATAGCGAAAGATCTT‑GCGCGGTATTCCTTGTCGGGATTGGCGGCAAGCTGAGCGATGGTCATCGCCACGACGTGCGCGCACCGGATTatgat                       <  1:333276/100‑1 (MQ=255)
                                                                             gCGGGCGATAGCGAAAGATCTT‑GCGCGGTATTCCTTGTCGGGATTGGCGGCAAGCTGAGCGATGGTCATCGCCACGACGTGCGCGCACCGGATTatgat                       >  2:1786080/1‑99 (MQ=255)
                                                                              cGGGCGATAGCGAAAGATCTT‑GCGCGGTATTCCTTGTCGGGATTGGCGGCAAGCTGAGCGATGGTCATCGCCACGACGTGCGCGCACCGGATTAtgatga                     >  2:422412/1‑100 (MQ=255)
                                                                               gggCGATAGCGAAAGATCTT‑GCGCGGTATTCCTTGTCGGGATTGGCGGCAAGCTGAGCGATGGTCATCGCCACGACGTGCGCGCACCGGATTATGATGAc                    >  2:743455/1‑100 (MQ=255)
                                                                                ggggATAGCGAACGAGC‑TCGCGCGGTGTTCCTTGTGGGGATTGGCGGCAAGGTGAGCGATGGTCATCGCTACGACGTGCGGGCACCGGATTatg                         <  2:1572002/91‑1 (MQ=255)
                                                                                ccgatctgCGAAAGATCTT‑GCGCGGTATTCCTTGTCGGGATGGGCGGCAAGCTGAGCGATGGTCATCGCCACGACGTGCGCGCACCGGATTATGATGAc                    <  2:1246117/92‑1 (MQ=255)
                                                                                  cGATAGCGAAAGATCTT‑GCGCGGTATTCCTTGTCGGGATTGGCGGCAAGCTGAGCGATGGTCATCGCCACGACGTGCGCGCACCGGATTATGATGACTgg                 >  1:2596987/1‑100 (MQ=255)
                                                                                    aTAGCGAAAGATCTT‑GCGCGGTATTCCTTGTCGGGATTGGCGGCAAGCTGAGCGATGGTCATCGCCACGACGTGCGCGCACCGGATTATGATGACTGGAg               <  2:1027318/100‑1 (MQ=255)
                                                                                    aTAGCGAAAGATCTT‑GCGCGGTATTCCTTGTCGGGATTGGCGGCAAGCTGAGCGATGGTCATCGCCACGACGTGCGCGCACCGGATTATGATGACTGGAg               >  1:2458910/1‑100 (MQ=255)
                                                                                     tatcGAAATATCTT‑GCGCGGTATTCCTTGTCGGGATTGGCGACAAGCTGAGCGATGGTCATCGCCACGACGTGCGCGCACCGGATTATGATGACTGGAGc              <  2:2590111/97‑1 (MQ=255)
                                                                                     tAGCGAAAGATCTT‑GCGCGGTATTCCTTGTCGGGATTGGCGGCAAGCTGAGCGATGGTCATCGCCACGACGTGCGCGCACCGGATTATGATGACTGGAGc              <  1:2227225/100‑1 (MQ=255)
                                                                                     tAGCGAAAGATCTT‑GCGCGGTATTCCTTGTCGGGATTGGCGGCAAGCTGAGCGATGGTCATCGCCACGACGTGCGCGCACCGGATTATGATGACTGGAGc              <  1:2997399/100‑1 (MQ=255)
                                                                                       gCGAAAGATCTT‑GCGCGGTATTCCTTGTCGGGATTGGCGGCAAGCTGAGCGATGGTCATCGCCACGACGTGCGCGCACCGGATTATGATGAcagatcgg             >  1:1246129/1‑92 (MQ=255)
                                                                                       gCGAAAGATCTT‑GCGCGGTATTCCTTGTCGGGATTGGCGGCAAGCTGAGCGATGGTCATCGCCACGACGTGCGCGCACCGGATTATGATGACTGGAGCAc            >  2:2399573/1‑100 (MQ=255)
                                                                                        cGAAAGATCTT‑GCGCGGTATTCCTTGTCGGGATTGGCGGCAAGCTGAGCGATGGTCATCGCCACGACGTGCGCGCACCGGATTATGATGACTGGAGCAcc           >  2:1598594/1‑100 (MQ=255)
                                                                                        cGAAAGATCTT‑GCGCGGTATTCCTTGTCGGGATTGGCGGCAAGCTGAGCGATGGTCATCACCAAAACATACGAGCACCGGATTATGATGACTGGAGCAcc           >  1:1152797/1‑100 (MQ=255)
                                                                                         gAAAGATCTT‑GCGCGGAATTCCTTGTCGGGATTGGCGGCAAGCTGAGCGATGGTCATCGCCACGACGTACGCGCAGCGGGTTATGATGGCTGGAGCAcgc          >  2:776837/1‑98 (MQ=255)
                                                                                           aaGATCTT‑TCGCGGTATTCCTTGTCGGGATTGGCGGCAAGCTGAGCGATGGTCATCGCCACGACGTGCGCGCACCGGATTATGATGACTGGAGCACCCCg        <  1:678509/100‑1 (MQ=255)
                                                                                            aGATCTT‑GCGCGGTATTCCTTGTCGGGATTGGCGGCAAGCTGAGCGATGGTCATCGCCACGACGTGCGCGCACCGGATTATGATGACTGGAGCACCCCGt       >  1:2838381/1‑100 (MQ=255)
                                                                                             gATCTT‑GCGCGGTATTCCTTGTCGGGATTGGCGGCAAGCTGAGCGATGGTCATCGCCACGACGTGCGCGCACCGGATTATGATGACTGGAGCACCCCGTc      <  1:95626/100‑1 (MQ=255)
                                                                                             gATCTT‑GCGCGGTATTCCATGTAGAGATTGGCGGCAAGCTGAGCGATGGTCATCGCCACGACGTGCGCGCACCGGATTATGATGACTGGAGCACCCCTTc      >  2:2657559/1‑100 (MQ=255)
                                                                                              aTCTT‑GCGCGGTATTCCTTGTCGGGATTGGCGGCAAGCTGAGCGATGGTCATCGCCACGACGTGCGCGCACCGGATTATGATGACTGGAGCACCCCGTc      <  1:743459/99‑1 (MQ=255)
                                                                                                  tGGCGCGGTATTCCTTGTCGGGATTGGCGGCAAGCTGAGCGATGGTCATCGCCACGACGTGCGCGCACCGGATTATGATGACTGGAGCACCCCGTCagag  >  1:454886/1‑100 (MQ=255)
                                                                                                   |                                                                                                  
CTGCCGGATCAGATCCACTTCGTACACAGCCAGGAGTTACTGTCTCGTTATCCGGATCTTGATGCCAAAGGGCGTGAGCGGGCGATAGCGAAAGATCTTGGCGCGGTATTCCTTGTCGGGATTGGCGGCAAGCTGAGCGATGGTCATCGCCACGACGTGCGCGCACCGGATTATGATGACTGGAGCACCCCGTCAGAG  >  NC_000913/3927635‑3927832

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑