Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,500,814 Δ1 bp 100% intergenic (‑23/‑446) yjgX ← / → yjgZ pseudogene fragment/uncharacterized protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,500,8110T.95.8% 511.4 / 11.7 119intergenic (‑20/‑449)yjgX/yjgZpseudogene fragment/uncharacterized protein
Reads supporting (aligned to +/- strand):  ref base T (1/3);  new base . (37/77);  total (39/80)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 130 total aligned reads displayed.

GTCCGCCAGAAAATCACCACGAATACCAGAATCGCGGCACTGACCAGAAAATGATAAACAGGAAACACGGGGCGGTTCACTTTGCTTTCTCCTGTAGTGTTTTCTGTAGGTTCTGAACCGGTTCTAGCGGGGTTGTGAGGCTGATGGCAATCGCCGGGCTCTTCCTGCATGTTACACTGGTACTGCAAGAGGGGG  >  NC_000913/4500712‑4500906
                                                                                                   |                                                                                               
  ccGCCAGAAAATCACCACGAATACCAGAATCGCGGCACTGACCAGAAAATGATAAACAGGAAACACGGGGCGGTTCACTTTGCTTTCTCCTGTAGTGttt                                                                                               >  2:880656/1‑100 (MQ=255)
   cGCCAGAAAATCACCACGAATACCAGAATCGCGGCACTGAACAGAAAATGATAAACAGGAAACACGGGGCGGTTCACTTTGCTTTCTCCTGTAGTGtttc                                                                                              >  2:2702150/1‑99 (MQ=255)
    gCCAGAAAATCACCACGAATACCAGAATCGCGGCACTGACCAGAAAATGATAAACAGGAAACACGGGGCGGTTCACTTTGCTTTCTCCTGTAGTGtttct                                                                                             >  2:1212302/1‑98 (MQ=255)
    gCCAGAAAATCACCACGAATACCAGAATCGCGGCACTGACCAGAAAATGATAAACAGGAAACACGGGGCGGTTCACTTTGCTTTCTCCTGTAGTGtttct                                                                                             >  2:1304547/1‑98 (MQ=255)
    gCCAGAAAATCACCACGAATACCAGAATCGCGGCACTGACCAGAAAATGATAAACAGGAAACACGGGGCGGTTCACTTTGCTTTCTCCTGTAGTGtttct                                                                                             >  2:628418/1‑98 (MQ=255)
    gCCAGAAAATCACCACGAATACCAGAATCGCGGCACTGACCAGAAAATGATAAACAGGAAACACGGGGCGGTTCACTTTGCTTTCTCCTGTAGTGtttct                                                                                             >  2:1958769/1‑98 (MQ=255)
     ccAGAAAATCACCACGAATACCAGAATCGCGGCACTGACCAGAAAATGATAAACAGGAAACACGGGGCGGTTCACTTTGCTTTCTCCTGTAGTGtttctg                                                                                            <  1:1425935/100‑4 (MQ=255)
      cAGAAAATCACCACGAATACCAGAACCACGGCACTGACCAGAAAATGATAAACAGGAAACACGGGGCGGCTCACTTTGCTTTCTCCTGTCGTGtttctgt                                                                                           >  2:1268913/1‑96 (MQ=255)
       aGAAAATCACCACGAATACCAGAATCGCGGCACTGACCAGAAAATGATAAACAGGAAACACGGGGCGTTTCACTTTGCTTTCTCCTGTAGTGTTTCTGTa                                                                                         <  1:2889702/100‑1 (MQ=255)
       aGAAAATCACCACGAATACCAGAATCGCGGCACTGACCAGAAAATGATAAACAGGAAACACGGGGCGGTTCACTTTGCTTTCTCCTGTAGTGTTTCTGTa                                                                                         <  2:2213596/100‑1 (MQ=255)
       aGAAAATCACCACGAATACCAGAATCGCGGCACTGACCAGAAAATGATAAACAGGAAACACGGGGCGGTTCACTTTGCTTTCTCCTGTAGTGTTTCTGTa                                                                                         <  1:1962976/100‑1 (MQ=255)
        gAAAATCACCACGAATACCAGAATCGCGGCACTGACCAGAAAATGATAAACAGGAAACACGGGGCGGTTCACTTTGCTTTCTCCTGTAGTGTTTCTGTAg                                                                                        >  1:2817231/1‑100 (MQ=255)
        gAAAATAACCACGAATACCAGAATCGCGGCACTGACCAGAAAATGATAAACAGGAAACACGGGGCGGTTCACTTTGCTTTCTACTGTAGTCtttctgt                                                                                           >  2:3032891/1‑94 (MQ=255)
             tCACCACGAATACCAGAATCGCGGCACTGACCAGAAAATGATAAACAGGAAACACGGGGCGGTTCACTTTGCTTTCTCCTGTAGTGTTTCTGTAGGTTCt                                                                                   <  1:1212313/100‑1 (MQ=255)
              caccacGAATACCAGAATCGCGGCACTGACCAGAAAATGATAAACAGGAAACACGGGGCGGTTCACTTTGCTTTCTCCTGTAGTGTTTCTGTAGGTTCTg                                                                                  >  2:1442790/1‑100 (MQ=255)
               accacGAATACCAGAATCGCGGCACTGACCAGAAAATGATAAACAGGAAACACGGGGCGGTTCACTTTGCTTTCTCCTGTAGTGTTTCTGTAGGTTCTGa                                                                                 >  1:1832759/1‑100 (MQ=255)
                ccacGAATACCAGAATCGCGGCACTGACCAGAAAATGATAAACAGGAAACACAGGGCGGATCACTTTGATTTCTCCTGTAGTGTTTCTGTAGGTTCTGaa                                                                                >  1:154474/1‑100 (MQ=255)
                 cacGAATACCAGAATCGCGGCACTGACCAGAAAATGATAAACAGGAAACACGGGGCGGTTCACTTTGCTTTCTCCTGTAGTGTTTCTGTAGGTTCTGa                                                                                 <  2:1842513/98‑1 (MQ=255)
                  acGAATACCAGAATCGCGGCACTGACCAGAAAATGATAAACAGGAAACACGGGGCGGTTCACTTTGCTTTCTCCTGTAGTGTTTCTGTAGGTTCTGAAcc                                                                              <  2:2844411/100‑1 (MQ=255)
                  acGAATACCAGAATCGCGGCACTGACCAGAAAATGATAAACAGGAAACACGGGGCGGTTCACTTTGCTTTCTCCTGTAGTGTTTCTGTAGGTTCTGAAcc                                                                              <  2:1679860/100‑1 (MQ=255)
                  acGAATACCAGAATCGCGGCACTGACCAGAAAATGATAAACAGGAAACACGGGGCGGTTCACTTTGCTTTCTCCTGTAGTGTTTCTGTAGGTTCTGAAcc                                                                              <  1:79769/100‑1 (MQ=255)
                   cGAATACCAGAATCGCGGCACTGACCAGAAAATGATAAACAGGAAACACGGGGCGGTTCACTTTGCTTTCTCCTGTAGTGTTTCTGTAGGTTCTGAACCg                                                                             >  2:1017600/1‑100 (MQ=255)
                    gAATACCAGAATCGCGGCACTGACCAGAAAATGATAAACAGGAAACACGGGGCGGTTCACTTTGCTTTCTCCTGTAGTGTTTCTGTAGGTTCTGAACCgg                                                                            <  2:590182/100‑1 (MQ=255)
                     aaTACCAGAATCGCGGCACTGACCAGAAAATGATAAACAGGAAACACGGGGCGGTTCACTTTGCTTTCTCCTGTAGTGTTTCTGTAGGTTCTGAACCGGt                                                                           <  2:2558176/100‑1 (MQ=255)
                      aaaCCAGAATCGCGGCACTTACCAGAAAATGATAAACAGGAAATACGGGGCGGTTCACTTTGCTTTCTCCTGTAGTGTTTCTGTAGGTTCTGAACCGGt                                                                           <  2:2919782/97‑1 (MQ=255)
                        aCCAGAATCGCGGCACTGACCAGAAAATGATAAACAGGAAACACGGGGCGGTTCACTTTGCTTTCTCCTGTAGTGTTTCTGTAGGTTCTGAACCGGTTCt                                                                        <  2:511787/100‑1 (MQ=255)
                         ccAGAATCGCGGCACTGACCAGAAAATGATAAACAGGAAACACGGGGCGGTTCACTTTGCTTTCTCCTGTAGTGTTTTCTGTAGGTTCTGAACCGGTTCt                                                                        >  2:1633042/1‑100 (MQ=255)
                         ccAGAATCGCGGCACTGACCAGAAAATGATAAACAGGAAACACGGGGCGGTTCACTTTGCTTTCTCCTGTAGTGTTTCTGTAGGTTCTGAACCGGTTCTa                                                                       >  2:3009879/1‑100 (MQ=255)
                          cAGAATCGCGGCACTGACCAGAAAATGATAAACAGGAAACACGGGGCGGTTCACTTTGCTTTCTCCTGTAGTGTTTCTGTAGGTTCTGAACCGGTTCTAg                                                                      >  2:2484267/1‑100 (MQ=255)
                            gAATCGCGGCACTGACCAGAAAATGATAAACAGGAAACACGGGGCGGTTCACTTTGCTTTCTCCTGTAGTGTTTCTGTAGGTTCTGAACCGGTTCTAGCg                                                                    <  1:2906983/100‑1 (MQ=255)
                              aTCGCGGCACTGACCAGAAAATGATAAACAGGAAACTCGGGGCGGTTCACTTTGCTTTCTCCTGTAGTGTTTCTGTAGGTTCTGAACCGGTTCTAGCggg                                                                  <  1:1944263/100‑1 (MQ=255)
                              aTCGCGGCACTGACAAGAAAATGATAAACAGGAAACACGGGGCGGTTCACTTTGCTTTCTCCTGTAGTGTTTCTGTAGGTTCTGAACCGGTTCTAGCggg                                                                  >  2:607837/1‑100 (MQ=255)
                               tCGCGGCACTGACCAGAAAATGATAAACAGGAAACACGGGGCGGTTCACTTTGCTTTCTCCTGTAGTGTTTCTGTAGGTTCTGAACCGGTTCTAGCgggg                                                                 <  1:191579/100‑1 (MQ=255)
                                  cgGCACTGACCAGAAAATGATAAACAGGAAACACGGGGCGGTTCACTTTGCTTTCTCCTGTAGTGTTTCTGTAGGTTCTGAACCGGTTCTAGCGGGGTtg                                                              <  1:546733/100‑1 (MQ=255)
                                    gCACTGACCAGAAAATGATAAACAGGAAACACGGGGCGGTTCACTTTGCTTTCTCCTGTAGTGTTTCTGTAGGTTCTGAACCGGTTCTAGCGGGGTtgtg                                                            >  2:1934967/1‑100 (MQ=255)
                                     cACTGACCAGAAAATGATAAACAGGAAACACGGGGCGGTTCACTTTGCTTTCTCCTGTGGTGTTTCTGTAGGTTCTGAACCGGTTCTAGCGGGGTTGTGa                                                           <  2:1647544/100‑1 (MQ=255)
                                      aCTGACCAGAAAATGATAAACAGGAAACACGTGGCGGTTCACTTTGCTTTCTCCTGTAGTGTTTCTGTAGGTTCTGAACCGGTTCTAGCGGGGTTGTGAg                                                          <  1:285033/100‑1 (MQ=255)
                                      aCTGACCAGAAAATGATAAACAGCAAACACGGGGCGGTTCACTTTGCTTTCTCCTGTAGTGTTTCTGTAGGTTCTGAACCGGTTCTAGCGGGGTtgtn                                                            <  2:434185/98‑2 (MQ=255)
                                        tGACCAGAAAATGATAAACAGGAAACACGGGGCGGTTCACTTTGCTTTCTCCTGTAGTGTTTCTGTAGGTTCTGAACCGGTTCTAGCGGGGTTGTGAGGc                                                        >  2:1416303/1‑100 (MQ=255)
                                         gACCAGAAAATGATAAACAGGATACACGGGGCGGTTCACTTTGCTTTCTCCTGTAGTGTTTCTGTAGGTTCTGAACCGGTTCTAGCGGGGTTGTGAGGc                                                        <  1:937070/99‑1 (MQ=255)
                                         gACCAGAAAATGATAAACAGGAAACACGGGGCGGTTCACTTTGCTTTCTCCTGTAGTGTTTTCTGTAGGTTCTGAACCGGTTCTAGCGGGGTTGTGAGGc                                                        <  2:853764/100‑1 (MQ=255)
                                         gACCAGAAAATGATAAACAGGAAACACGGGGCGGTTCACTTTGCTTTCTCCTGTAGTGTTTCTGTAGGTTCTGAACCGGTTCTAGCGGGGTTGTGAGGCt                                                       >  2:1408032/1‑100 (MQ=255)
                                         gACCAGAAAATGATAAACAGGAAACACGGGGCGGTTCACTTTGCTTTCTCCTGTAGTGTTTCTGTAGGTTCTGAACCGGTTCTAGCGGGGTTGTGAGGCt                                                       >  1:2879502/1‑100 (MQ=255)
                                          aCCAGAAAATGATAAATAGGAAACACGGGGCGGTTCACTTTGCTTTCTCCTGTAGTGTTTCTGTAGGTTCTGAACCGGTTCTAGCGGGGTTGTGAGGCt                                                       <  1:1464768/99‑1 (MQ=255)
                                           ccAGAAAATGATAAACAGGAAACACGGGGCGGTTCACTTTGCTTTCTCCTGTAGTGTTTCTGTAGGTTCTGAACCGGTTCTAGCGGGGTTGTGAGGCTGa                                                     >  1:1885191/1‑100 (MQ=255)
                                            cAGAAGATGATAATCAGGAGACTCGGGTCAGTTTGCTTTGCTTTCTCCTGTAGTGTTTCTGTAGGTTCTGAACCGTTTTTAGCGATGTTGTTAGGc                                                        <  2:1243901/96‑1 (MQ=255)
                                             aGAAAATGATAAACAGGAAACACGGGGCGGTTCACTTTGCTTTCTCCTGTAGTGTTTCTGTAGGTTCTGAACCGGTTCTAGCGGGGTTGTGAGGCTGATg                                                   >  1:37252/1‑100 (MQ=255)
                                              gAAAATGATAAACAGGAAACACGGGGCGGTTCACTTTGCTTTCTCCTGTAGTGTTTCTGTAGGTTCTGAACCGGTTCTAGCGGGGTTGTGAGGCTGATgg                                                  <  1:2745109/100‑1 (MQ=255)
                                              gAAAATGATAAACAGGAAACACGGGGCGGTTCACTTTGCTTTCTCCTGTAGTATTTCTGTAGGTTCTAAACCGGTTCTAGCGGGGTTGAGAGACTGATgg                                                  >  1:2223510/1‑100 (MQ=255)
                                               aaaaTGATAAACAGGAAACACGGGGCGGTTCACTTTGCTTTTTCCTGTAGTGTTTCTGTAGGTTCTGAACCGGTTCTAGCGGGGTTGTGAGGCTGATGGc                                                 <  1:446296/100‑1 (MQ=255)
                                               aaaaTGATAAACAGGAAACACGGGGCGGTTCACTTTGCTTTCTCCTGTAGTGTTTCTGTAGGTTCTGAACCGGTTCTAGCGGGGTTGTGAGGCTGATGGc                                                 <  1:836932/100‑1 (MQ=255)
                                                aaaTGATAAACAGGAAACACGGGGCGGTTCACTTTGCTTTCTCCTGTAGTGTTTCTGTAGGTTCTGAACCGGTTCTAGCGGGGTTGTGAGGCTGATgg                                                  <  1:2955785/98‑1 (MQ=255)
                                                  aTGATAAACAGGAATCTCGGGGCGGTTCACTTTGCTTTCTCCTGTAGTGTTTTCTGTAGGTTCTGAACCGGTTCTAGCGGGGTTGTGAGGCTGATGGCaa                                               <  1:169936/100‑1 (MQ=255)
                                                  aTGATAAACAGGAAACACGGGGCGGTTCACTTTGCTTTCTCCTGTAGTGTTTCTGTAGGTTCTGAACCGGTTCTAGCGGGGTTGTGAGGCTGATGGCAAt                                              <  1:1442804/100‑1 (MQ=255)
                                                      tAAACAGGAAACACGGGGCGGTTCACTTTGCTTTCTCCTGTAGTGTTTCTGTAGGTTCTGAACCGGTTCTAGCGGGGTTGTGAGGCTGATGGCAATCGcc                                          <  2:2703603/100‑1 (MQ=255)
                                                      tAAACAGGAAACACGGGGCGGTTCACTTTGCTTTCTCCTGTAGTGTTTCTGTAGGTTCTGAACCGGTTCTAGCGGGGTTGTGAGGCTGATGGCAATCGcc                                          <  1:1304559/100‑1 (MQ=255)
                                                        aaCAGGAAACACGGGGCGGTTCACTTTGCTTTCTCCTGTAGTGTTTCTGTAGGTTCTGAACCGGTTCTAGCGGGGTTGTGAGGCTGATGGCAATCGCCgg                                        <  1:1149694/100‑1 (MQ=255)
                                                         aCAGGAAACACGGGGCGGTTCACTTTGCTTTCTCCTGTAGTGTTTCTGTAGGTTCTGAACCGGTTCTAGCGGGGTTGTGAGGCTGATGGCAATCGCCggg                                       <  1:1612239/100‑1 (MQ=255)
                                                          cAGGAAACACGGGGCGGTTCACTTTGCTTTCTCCTGTAGTGTTTCTGTAGGTTCTGAACCGGTTCTAGCGGGGTTGTGAGGCTGATGGCAATCGCCGGGc                                      >  2:908762/1‑100 (MQ=255)
                                                            ggAAACACGGGGCGGTTCACTTTGCTTTCTCCTGTAGTGTTTCTGTAGGTTCTGAACCGGTTCTAGCGGGGTTGTGAGGCTGATGGCAATCGCCGGGctc                                    >  2:760971/1‑100 (MQ=255)
                                                             gAAACACGGGGCGGTTCACTTTGCTTTCTCCTGTAGTGTTTCTGTAGGTTCTGAACCGGTTCTAGCGGGGTTGTGAGGCTGATGGCAATCGCCGGGctct                                   <  1:456257/100‑1 (MQ=255)
                                                             gAAACACGGGGCGGTTCACTTTGCTTTCTCCTGTAGTGTTTCTGTAGGTTCTGAACCGGTTCTAGCGAGATTGTGAGGCTGATGGCAATCGCCGGGctct                                   >  1:827694/1‑100 (MQ=255)
                                                              aaaCACGGGGCTGTTCACTTTGCTTTCTCCTGTAGTGTTTCTGTAGGTTCTGAACCGGTTCTAGCGGGGTTGTGAGGCTGATGGCAATCGCCGGGCTCtt                                  <  1:3032925/100‑1 (MQ=255)
                                                               aaaaaggggCGGTTCACTTTGCTTTTTCCTGTAGTGTTTCTGTAGGTTCTGAACCGGTTCTAGCGGGGTTGTGAGGATGATGGCAATCg                                            <  2:867034/84‑1 (MQ=255)
                                                                  acGGGGCGGTTCACTTTGCTTTCTCCTGTAGTGTTTCTGTAGGTTCTGAACCGGTTCTAGCGGGGTTGTGAGGCTGATGGCAATCGCCGGGCTCTTCCTg                              <  1:1246313/100‑1 (MQ=255)
                                                                   cGGGGCGGTTCACTTTGCTTTCTCCTGTAGTGTTTCTGTAGGTTCTGAACCGGTTCTAGCGGGGTTGTGAGGCTGATGGCAATCGCCGGGCTCTTCCTGc                             <  1:1038038/100‑1 (MQ=255)
                                                                     gggCGGTTCACTTTGCTTTCTCCTGTAGTGTTTCTGTAGGTTCTGAACCGGTTCTAGCGGGGTTGTGAGGCTGATGGCAATCGCCGGGCTCTTCCTGCAt                           <  1:2456035/100‑1 (MQ=255)
                                                                       gctgTTCACTTTGCTATCTCCTGTAGTGTTTCTGTAGGTTCTGAACCAGTTCTAGCGGGGTTGTGAGGCTGATCGCAATCGCCGGGCTCTTCCTGCATGt                         <  2:2104624/97‑1 (MQ=255)
                                                                        cGGTTCACTTTGCTTTCTCCTGTAGTGTTTCTGTAGGTTCTGAACCGGTTCTAGCGGGGTTGTGAGGCTGATGGCAATCGCCGGGCTCTTCCTGCATGtt                        <  1:1069441/100‑1 (MQ=255)
                                                                          gTTCACTTTGCTTTCTCCTGTAGTGTTTCTGTAGGTTCTTAACCGGTTCTAGCGGGGTTGTGAGGCTGATGGCAATCGCCGGGCTCTTCCTGCATGTTa                       <  2:27966/99‑1 (MQ=255)
                                                                            tCACTTTGCTTTCTCCTGTAGTGTTTCTGTAGGTTCTGAACCGGTTCTAGCGGGGTTGTGAGGCTGATGGCAATCGCCGGGCTCTTCCTGCATGTTacac                    <  1:1338340/100‑1 (MQ=255)
                                                                            tCACTTTGCTTTCTCCTGTAGTGTTTCTGTAGGTTCTGAACCGGTTCTAGCGGGGTTGTGAGGCTGATGGCAATCGCCGGGCTCTTCCTGCATGTTacac                    <  2:1959571/100‑1 (MQ=255)
                                                                              aCTTTGCTTTTTCTTGTAGTGTTTCTGTAGGTTCTGAACCGGTTCTAGCGGGGTTGTGAGGCTGATGGCAATCGCCGGGCTCTTCCTGCATGTTACACTg                  <  1:444029/100‑1 (MQ=255)
                                                                               cTTTGCTTTCTCCTGTATTGTTTCTGTAGGTTCTGAACCGGTTCTAGCGGGGTTGTGAGGCTGATGGCAATCGCCGGGCTCTTCCTGCATGTTACACTgg                 <  1:1156050/100‑1 (MQ=255)
                                                                               cTTTGCTTTCTCCTGTAGTGTTTCTGTAGGTTCTGAACCGGTTCTAGCGGGGTTGTGAGGCTGATGGCAATCGCCGGGCTCTTCCTGCATGTTACACTgg                 <  1:1536046/100‑1 (MQ=255)
                                                                               cTTTGCTTTCTCCTGTAGTGTTTCTGTAGGTTCTGAACCGGTTCTAGCGGGGTTGTGAGGCTGATGGCAATCGCCGGGCTCTTCCTGCATGTTACACTgg                 <  2:2150394/100‑1 (MQ=255)
                                                                               cTTTGCTTTCGCCTGTAGTGTTTCTGTAGGTCCTGAACCGGTTCTAGCGGGGTTGTGAGGCTGATGCCAATCGCCGGGCTCTTCCTGCATGTTACACTgg                 <  2:1986778/100‑1 (MQ=255)
                                                                                tttGCTTTCTCCTGTAGTGTTTCTGTAGGTTCTGAACCGGTTCTAGCGGGGTTGTGAGGCTGATGGCAATCGCCGGGCTCTTCCTGCATGTTACACTgg                 >  2:1148354/1‑99 (MQ=255)
                                                                                tttGCTTTCTCCTGTAGTGTTTCTGTAGGTTCTGAACCGGTTCTAGCGGGGTTGTGAGGCTGATGGCAATCGCCGGGCTCTTCCTGCATGTTACACTGGt                <  2:1981596/100‑1 (MQ=255)
                                                                                tttGCTTTCTCCTGTAGTGTTTCTGTAGGTTCTGAACCGGTTCTAGCGGGGTTGTGAGGCTGATGGCAATCGCCGGGCTCTTCCTGCATGTTACACTGGt                <  1:1408045/100‑1 (MQ=255)
                                                                                   gCTTTCTCCTGTAGTGTTTCTGTAGGTTCTGAACCGGTTCTAGCGGGGTTGTGAGGCTGATGGCAATCGCCGGGCTCTTCCTGCATGTTACACTGGTACt             >  1:1528981/1‑100 (MQ=255)
                                                                                   gCTTTCTCCTGTAGTGTTTCTGTAGGTTCTGAACCGGTTCTAGCGGGGTTGTGAGGCTGATGGCAATCGCCGGGCTCTTCCTGCATGTTACACTGGTACt             >  1:2357566/1‑100 (MQ=255)
                                                                                    cTTTCTCCTGTAGTGTTTCTGTAGGTTCTGAACCTGTTCTAGCGGGGTTGTGAGGCTGATGGCAATCGCCGGGCTCTTCCTGCATGTTGCACCGGTACTg            <  2:1584261/100‑1 (MQ=255)
                                                                                    cTTTCTCCTGTAGTGTTTCTGTAGGTTCTGAACCGGTTCTAGCGGGGTTGTGAGGCTGATGGCAATCGCCGGGCTCTTCCTGCATGTTACACTGGTACTg            <  1:1068896/100‑1 (MQ=255)
                                                                                    cTTTCTCCTGTAGTGTTTCTGTAGGTTCTGAACCGGTTCTAGCGAGGTTGTGAGGCTGATGGCAATCGCCGGGCTCTTCCTGCATGTTACACTGGTACTg            >  1:1074651/1‑100 (MQ=255)
                                                                                    cTTTCTCCTGTAGTGTTTCTGTAGGTTCTCAACCGGTTCTAGCGTGGTTGTGAGGCTGATGGCAATCGCCGGGCTCTTCCTGCATGTTACACTGGTACTg            <  2:1097844/100‑1 (MQ=255)
                                                                                     tttCTCCTGTAGTGTTTCTGTAGGTTCTGAACCGGTTCTAGCGGGGTTGTGAGGCTGATGGCAATCGCCGGGCTCTTCCTGCATGTTACACTGGTACTGc           <  2:2914520/100‑1 (MQ=255)
                                                                                      ttCTCCTGTAGTGTTTCTGTAGGTTCTGAACCGGTTCTAGCGGGGTTGTGAGGCTGATGGCAATCGCCGGGCTCTTCCTGCATGTTACACTGGTACTGc           >  2:1151358/1‑99 (MQ=255)
                                                                                      ttCTCCTGTAGTGTTTCTGTAGGTTCTGAACCGGTTCTAGCGGGGTTGTGAGGCTGATGGCAATCGCCGGGCTCTTCCTGCATGTTACACTGGTACTGCa          >  2:548841/1‑100 (MQ=255)
                                                                                          ccTGTAGTGTTTCTGTAGGTTCTGAACCGGTTCTAGCGGGGTTGTGAGGCTGATGGCAATCGCCGGGCTCTTCCTGCATGTTACACTGGTACTGCAagag      >  2:180800/1‑100 (MQ=255)
                                                                                          ccTGTAGTGTTTCTGTAGGTTCTGAACCGGTTCTAGCGGGGTTGTGAGGCTGATGGCAATCGCCGGGCTCTTCCTGCATGTTACACTGGTACTGCAaga       >  2:1267589/1‑99 (MQ=255)
                                                                                           cTGTAGTGTTTCTGTAGGTTCTGAACCGGTTCTAGCGGGGTTGTGAGGCTGATGGCAATCGCCGGGCTCTTCCTGCATGTTACACTGGTACTGCAAGAgg     >  1:323528/1‑100 (MQ=255)
                                                                                           cTGTAGTGTTTCTGTAGGTTCTGAACCGGTTCTAGCGGGGTTGTGAGGCTGATGGCAATCGCCGGGCTCTTCCTGCATGTTACACTGGTACTGCAAGAgg     >  2:2478287/1‑100 (MQ=255)
                                                                                            tGTAGTGTTTCTGTAGGTTCTGAACCGGTTCTAGCGGGGTTGTGAGGCTGATGGCAATCGCCGGGCTCTTCCTGCATGTTACACTGGTACTGCAAGAggg    <  2:907929/100‑1 (MQ=255)
                                                                                            tGTAGTGTTTCTGTAGGTTCTGAACCGGTTCTAGCGGGGTTGTGAGGCTGATGGCAATCGCCGGGCTCTTCCTGCATGTTACACTGGTACTGCAAGAggg    <  1:2579129/100‑1 (MQ=255)
                                                                                            tGTAGTGTTTCTGTAGGTTCTGAACCGGTTCTAGCGGGGTTGTGAGGCTGATGGCAATCGCCGGGCTCTTCCTGCATGTTACACTGGTACTGCAAGAggg    <  2:621442/100‑1 (MQ=255)
                                                                                            tGTAGTGTTTATGTAGGTTCTGAACCGGTTCTAGCGGGGTTGTGAGGCTGATGGCAATCGCCGGGCGCTGCCTGCATGTTACACTGGTACTGCAAGAggn    <  2:1992515/100‑2 (MQ=255)
                                                                                             gTAGTGTTTCTGTAGGTTCTGAACCGGTTCTAGCGGGGTTGTTTTGCTGATGGCAATCGCCGGGCTCTTCCTGCATGTTACACTGGTACTGCAAGAggg    <  2:908641/99‑1 (MQ=255)
                                                                                             gTAGTGTTTCTGTAGGTTCTGAACCGGTTCTAGCGGGGTTGTGAGGCTGATGGCAATCGCCGGGCTCTTCCTGCATGTTACACTGGTACTGCAAGAgggg   >  1:1651234/1‑100 (MQ=255)
                                                                                              tAGTGTTTCTGTAGGTTCTGAACCGGTTCTAGCGGGGTTGTGAGGCTGATGGCAATCGCCGGGCTCTTCCTGCATGTTACACTGGTACTGCAAGAggggg  <  1:2179983/100‑1 (MQ=255)
                                                                                                   |                                                                                               
GTCCGCCAGAAAATCACCACGAATACCAGAATCGCGGCACTGACCAGAAAATGATAAACAGGAAACACGGGGCGGTTCACTTTGCTTTCTCCTGTAGTGTTTTCTGTAGGTTCTGAACCGGTTCTAGCGGGGTTGTGAGGCTGATGGCAATCGCCGGGCTCTTCCTGCATGTTACACTGGTACTGCAAGAGGGGG  >  NC_000913/4500712‑4500906

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: