Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 107,553 (T)9→8 100% intergenic (+79/‑152) lpxC → / → secM UDP‑3‑O‑acyl N‑acetylglucosamine deacetylase/regulator of secA translation

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913107,5450T.90.2% 337.4 / 32.4 92intergenic (+71/‑160)lpxC/secMUDP‑3‑O‑acyl N‑acetylglucosamine deacetylase/regulator of secA translation
Reads supporting (aligned to +/- strand):  ref base T (8/1);  new base . (46/37);  total (54/38)
Fisher's exact test for biased strand distribution p-value = 7.56e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 107 total aligned reads displayed.

TCAAAGCGCCTTCAGCTGTACTGGCATAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCGTTTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTCCGCACAACTTATCTTCATTCGTGCTGTGG  >  NC_000913/107446‑107638
                                                                                                   |                                                                                             
tCAAAGCGCCTTCAGCTGTACTGGCATAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCGt                                                                                               <  2:1746767/100‑1 (MQ=255)
   naGCGCCTTCAGCTGTACTGGCATAACGACATTGATACTGTCGTATAAAATTCGACTGGCAAATCTGGCAATCTCTCCGGCCAGGTGAACCAGTCGttt                                                                                             >  2:1819746/2‑99 (MQ=255)
   aaGCGCCTTCAGCTGTACTGGCATAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACAAGTCGtttt                                                                                            >  1:2103038/1‑100 (MQ=255)
      cgcCTTCAGCTGTACTGGCATAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCGttttttt                                                                                         <  1:1493047/100‑1 (MQ=255)
       gcCTTCAGCTGTACTGGCATAACGACATTTATACTTTCGTATAAAATTCGACTGGCAAATCTTGCACTCTCTCCGGCCAGGTGAACCAGTCGttttttt                                                                                         <  2:2902203/99‑1 (MQ=255)
       gcCTTCAGCTGTACTGGCATAACGACATTTATACTGTCGTATAAAATTTGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCGtttttttt                                                                                        <  1:2429184/100‑1 (MQ=255)
       gcCTTCAGCTGTACTGGCATAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCGtttttttt                                                                                        <  2:1739517/100‑1 (MQ=255)
       gcCTTCAGCTGTACTGGCATAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCGtttttttt                                                                                        >  1:436694/1‑100 (MQ=255)
        ccTTCAGCTGTACTGGCATAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCGttttttttt                                                                                       <  2:608492/100‑1 (MQ=255)
        ccTTCAGCTGTACTGGCATAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCGttttttttg                                                                                       <  2:2981081/100‑2 (MQ=255)
        ccTTCAGCTGTACTGGCATAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCGttttttttg                                                                                       >  2:628210/1‑99 (MQ=255)
        ccTTCAGCTGTACTGGCATAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCGGGCACTCTCTCCGGCCAGGTGAACCAGTCGttttttttg                                                                                       <  1:771225/100‑2 (MQ=255)
           tCAGCTGTACTGGCATAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCGttttttttgaat                                                                                    >  1:1735485/1‑96 (MQ=255)
             aGCTGTACTGGCATAACGACATTTATATTGTCGTATAAAATTCGACTGGTAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCGTTTTTTTTGAAttt                                                                                 <  1:2383700/100‑1 (MQ=255)
             aGCTGTACTGGCATAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCGTTTTTTTTGAAttt                                                                                 >  1:2246983/1‑100 (MQ=255)
              gCTGTACTGGCATAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTGGTTTTTTTTTAAtttt                                                                                >  2:1771338/1‑100 (MQ=255)
              gCTGTACTGGCATAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCGTTTTTTTTGAAtttt                                                                                >  1:1448508/1‑100 (MQ=255)
               ccgTACTGGCATAACGACATTTATACTGTCGTATAAAATTCGAGTGAAAAATCTGGCACTGTCTTCGGCCATGTGAACCAGTCGTTTTTTTTGAAtttt                                                                                <  2:742976/97‑1 (MQ=255)
               cTGTACTGGCATAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGTCACTCTCTCCGGCCAGGTGAACCAGTCGTTTTTTTTGAATTTta                                                                               <  2:768782/100‑1 (MQ=255)
               cTGTACTGGCATAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCGTTTTTTTTGAATTTta                                                                               >  2:1056863/1‑100 (MQ=255)
                   aCTGGCATAACGACATTTTTACTGTCGTATAAATTTCTTTTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCGTTTTTTTTGAATTTTATAag                                                                           <  1:780374/100‑1 (MQ=255)
                     tGGCATAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCGTTTTTTTTGAATTTTATAagag                                                                         <  1:2946220/100‑1 (MQ=255)
                      ggCATAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCGTTTTTTTTGAATTTTATAagag                                                                         >  1:1895172/1‑99 (MQ=255)
                      ggCATAACGACATTTATACTGTCGTATAAAATTCGACAGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCGTTTTTTTTGAATTTTATAAGAGc                                                                        >  2:1615298/1‑100 (MQ=255)
                        cATAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCGTTTTTTTTGTATTTTATAAGAGCta                                                                      >  2:210471/1‑100 (MQ=255)
                          tAACGACCTTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCGTTTTTTTTGAATTTTATAAGAGCtata                                                                    >  1:114960/1‑100 (MQ=255)
                           aaCGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCGTTTTTTTTGAATTTTATAAGAGCTATaa                                                                   >  2:1469452/1‑100 (MQ=255)
                            aCGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCGGGTGAACCAGTCGTTTTTTTTGAATTTTATAAGAGCTATaaa                                                                  >  2:2126032/1‑100 (MQ=255)
                            aCGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCGTTTTTTTTGAATTTTATAAGAGCTATaaa                                                                  >  2:29185/1‑100 (MQ=255)
                             cGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCGTTTTTTTTGAATTTTATAAGAGCTATaaaa                                                                 <  1:473069/100‑1 (MQ=255)
                             cGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCGTTTTTTTTGAATTTTATAAGAGCTATaaaa                                                                 >  2:2762613/1‑100 (MQ=255)
                                cATTTATACTGTCGTATAAAAATCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCGTTTTTTTTGAATTTTATAAGAGCTATAAAAAAc                                                              >  2:1000968/1‑100 (MQ=255)
                                  tttATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCGTTTTTTTTTAATTTTATAAGAGCTATAAAAAACgg                                                            <  2:2909601/100‑1 (MQ=255)
                                     ataCTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCGTTTTTTTTGAATTTTATAAAAGCTATAAAAAACAGTGc                                                         >  1:666736/1‑100 (MQ=255)
                                      taCTGTCGTATAAAATTCGACTGGCAAATTTGGCACTCTCTCCGGCCAGGTGAACCAGTCGTTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGc                                                         <  1:2750744/99‑1 (MQ=255)
                                        cTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCGTTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGaa                                                      >  2:2141882/1‑100 (MQ=255)
                                          gTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCGTTTTTTTTGATTTTTATAAGAGCTATAAAAAACGGTGCGAACg                                                    >  2:2072673/1‑100 (MQ=255)
                                           tCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCGTTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGc                                                   <  1:2696390/100‑1 (MQ=255)
                                              tataAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCGTTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGt                                                >  1:1048525/1‑100 (MQ=255)
                                              tataAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCGTTTTTTTTGAATTTTATAAAAGCTATAAAAAACGGTGCGAACGCTGt                                                >  1:2716165/1‑100 (MQ=255)
                                               ataAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCGTTTTTTTTTTTTTTTTTTAGACCTCGAAAAAACGGTGCGAAGGCt                                                  >  2:560214/1‑97 (MQ=255)
                                                taAAATTCGACTTGCAAATCTGGCACTCTTTCCGGCCAGGTGAACCAGTCGTTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGttt                                              <  1:737993/100‑1 (MQ=255)
                                                taAAATTCGACTGGGAAATCTGGCACTTTCTCCGGTCAGGTGAACCAGTCGTTTTTTTTGAATTTGATAAGAGCTATAAAAAACGGTGCGAAGGCTGttt                                              <  2:18263/100‑1 (MQ=255)
                                                taAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCGTTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGttt                                              <  2:1688250/100‑1 (MQ=255)
                                                taAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCGTTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGttt                                              <  2:32901/100‑1 (MQ=255)
                                                 aaaaTTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCGTTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGtttt                                             <  2:2466242/100‑1 (MQ=255)
                                                  aaaTTCGACTGGCAAATCTGGCACTTTCTCCGGTCAGGTGAACCAGTCGTTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTc                                            <  1:346825/100‑1 (MQ=255)
                                                  aaaTTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCGTTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTc                                            >  1:730070/1‑100 (MQ=255)
                                                  aaaTTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCGTTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTc                                            <  1:2264226/100‑1 (MQ=255)
                                                   ttccgatcTGGCTAATCTGGCATTTTCTCCGGCCAGGTGAACCAGTCGTTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTc                                            <  1:1285685/92‑1 (MQ=255)
                                                   aaTTCTACTGGTAAATCTGGCACTCTCTCCTGTCAGGTGAACCAGTCGTTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTc                                            <  1:1151935/99‑1 (MQ=255)
                                                   aaTTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCGTTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCt                                           >  1:894445/1‑100 (MQ=255)
                                                   aaTTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCGTTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCt                                           <  1:168876/100‑1 (MQ=255)
                                                     ttCGATTTGCAAATCTGGCTCTCTCTCCGGCCAGGTGAACCAGTCGTTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTa                                         <  1:2923116/100‑1 (MQ=255)
                                                     ttCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCGTTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTa                                         >  1:2160323/1‑100 (MQ=255)
                                                     gatctcTGGCAAATCTGGCACTCTTTCCGGCCAGGTGAACCAGTCGTTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTa                                         <  1:1418192/95‑1 (MQ=255)
                                                      tCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCGTTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTaa                                        >  1:2203925/1‑100 (MQ=255)
                                                       cGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCGTTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAg                                       <  1:2190951/100‑1 (MQ=255)
                                                       cGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCGTTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAg                                       <  2:1154756/100‑1 (MQ=255)
                                                       cGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCGTTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAg                                       >  1:581345/1‑100 (MQ=255)
                                                       cGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCGTTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAg                                       <  1:1811036/100‑1 (MQ=255)
                                                       cGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCGTTTTTTTTGAATTTTATAAAAGCAATAAAAAACAGTACGAAAGCTGTTTTCTTaa                                        >  1:461513/1‑99 (MQ=255)
                                                        gACTGGCAAATCTGGCACTCTCTCCGGCCATGTGAACCAGTCGTTTTTTTTGAATTTTATAAAAACTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGc                                      >  1:1065242/1‑100 (MQ=255)
                                                        gACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCGTTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGc                                      >  1:207655/1‑100 (MQ=255)
                                                        gACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCGTTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGc                                      <  2:795940/100‑1 (MQ=255)
                                                        gACTGGCAAATCTGGCACTCTCGCCGTCCAGGTGAACCAGTCGTTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGc                                      <  2:1565382/100‑1 (MQ=255)
                                                          cTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCGTTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAgatc                                    >  2:1418178/1‑97 (MQ=255)
                                                          cTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCGTTTTTTTTGAATATTATAAGAGCTATAAAAAACGGTGAGAACGCTGTTTTCTTAAGCAc                                    >  1:2383390/1‑100 (MQ=255)
                                                            ggCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCGTTTTTTTTTTTATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACt                                   >  2:55750/1‑100 (MQ=255)
                                                            ggCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCGTTTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACt                                   >  1:1953603/1‑100 (MQ=255)
                                                            ggCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCGTTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTcagatcggaa                                   >  2:1285673/1‑90 (MQ=255)
                                                            ggCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCGTTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACtt                                  >  1:615940/1‑100 (MQ=255)
                                                            ggCAAATCTGGCACTCACTCCAGCCAAGTGAACCAGTCGTTTTTTTTGCATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACt                                   >  2:1881851/1‑99 (MQ=255)
                                                              naaaTCTGGCACTCTCTCCGGCCAGGGGAACCAGTCGTTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTATTAAGCACtttt                                >  1:701177/2‑100 (MQ=255)
                                                               aaaTCTGGCACTCTCTCCGGCCAGGTGAACCAGTCGTTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTc                               <  2:2893128/100‑1 (MQ=255)
                                                               aaaTCTGGCACTCTCTCCGGCCAGGTGAACCAGTCGTTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTc                               <  1:3046018/100‑1 (MQ=255)
                                                                 aTCTGGCACTCTCTCCGGCCAGGTGAACCAGTCGTTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTCCg                             >  1:987442/1‑100 (MQ=255)
                                                                 aTCTGGCACTCTCTCCGGCCAGGTGAACCAGTCGTTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCGGTTTTCTTAAGCACTTTTCCg                             >  2:1897060/1‑100 (MQ=255)
                                                                  tCTGGCACTCTCTCCGGCCAGGTGAACCAGTCGTTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTCCGc                            <  2:730066/100‑1 (MQ=255)
                                                                   cTGGCACTCTCTCCGGCCAGGTGAACCAGTCGTTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTCCGca                           <  2:1976526/100‑1 (MQ=255)
                                                                       cACTCTCTCCGGCCAGGTGAACCAGTCGTTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTCCGCACAAc                       >  2:406740/1‑100 (MQ=255)
                                                                       cACTCTCTCCGGCCAGGTGAACCAGTCGTTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTCCGCACAAc                       <  2:897103/100‑1 (MQ=255)
                                                                        aCTCTCTCCGGCCAGGTGAACCAGTCGTTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTCCGCACAACt                      >  1:2819849/1‑100 (MQ=255)
                                                                            tctcCGGCCAGGTGAACCAGTCGTTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTCCGCACAACTTATc                  <  2:2208143/100‑1 (MQ=255)
                                                                             ctcCGGCCAGGTGAACAAGTCGATTTTTTTGAATTTTATAAGAGCTATAAAAAAAGGTGCGAACGCTGTTTTCTTAAGCACTTTTACGCACAACTTATCt                 >  2:886996/1‑100 (MQ=255)
                                                                              tcCGGCCAGGTGAACCAGTCGTTTTTTTTTTTTTTTTTAAAAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTCCGCACAACTTCTCtt                >  2:2562163/1‑100 (MQ=255)
                                                                              tcCGGCCAGGTGAACCAGTCGTTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTCCGCACAACTTATCtt                >  2:1259092/1‑100 (MQ=255)
                                                                              tcCGGCCAGGTGAACCAGTCG‑‑TTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTCCGCACAACTTATCTTc               >  1:1750685/1‑100 (MQ=255)
                                                                                cGGCCAGGTGAACCAGTCGTTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTCCGCACAACTTATCTTCa              <  2:903580/100‑1 (MQ=255)
                                                                                  gCCAGGTGAACCAGTCGTTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTCCGCACAACTTATCTTCAtt            <  2:2296213/100‑1 (MQ=255)
                                                                                    cAGGTGAACCAGTCGTTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTCCGCACAACTTATCTTCATTCg          >  1:2462265/1‑100 (MQ=255)
                                                                                     aGGTGAACCAGTCGTTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTCCGCACAACTTATCTTCATTCGt         >  1:2409527/1‑100 (MQ=255)
                                                                                      ggTGAACCAGTCGTTTTTTTTTTTTTTTTTAAGAGCTATAAAAAATAGTGCAAAAGCTGTTTTCTTAAGCTCTTTTTCGCACAACTTTTCTTCAtt            >  2:1801103/1‑96 (MQ=255)
                                                                                      ggTGAACCAGTCGTTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTCCGCACAACTTATCTTCATTCGTg        >  2:285098/1‑100 (MQ=255)
                                                                                       gTGAACCTGTCGTTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTCCGCACAACTTATCTTCATTCGTGc       <  1:2367617/100‑1 (MQ=255)
                                                                                        tGAACCAGTCGTTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTCCGCACAACTTATCTTCATTCGTGCt      >  2:1661075/1‑100 (MQ=255)
                                                                                         gAACCAGTCGTTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTCCGCACAACTTATCTTCATTCGTGCtg     >  1:2141128/1‑100 (MQ=255)
                                                                                           aCCAGTCGTTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTCCGCACAACTTATCTTCATTCGTGCtgtg   <  2:1895149/100‑1 (MQ=255)
                                                                                            ccAGTCGTTATTTTTTAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTTCGCACAACTTATCTTCATTCGTGCtgt    >  2:163294/1‑98 (MQ=255)
                                                                                            ccAGTCGTTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTCCGCACAACTTATCTTCATTCGTGCTGTgg  >  1:2630844/1‑100 (MQ=255)
                                                                                                   |                                                                                             
TCAAAGCGCCTTCAGCTGTACTGGCATAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCGTTTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTCCGCACAACTTATCTTCATTCGTGCTGTGG  >  NC_000913/107446‑107638

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: