Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,073,307 Δ1 bp 100% coding (705/1149 nt) rutA ← pyrimidine oxygenase, FMN‑dependent

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,073,3050A.96.2% 449.9 / 8.0 104coding (707/1149 nt)rutApyrimidine oxygenase, FMN‑dependent
Reads supporting (aligned to +/- strand):  ref base A (2/1);  new base . (49/51);  total (52/52)
Fisher's exact test for biased strand distribution p-value = 6.18e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.92e-01

Only 100 of 109 total aligned reads displayed.

GTCGGTTTCATCGGCAATCACCATAAACAACACATAAGAGCCAACGTCGCGTCCGGTTTGCTCTGCGGCCTGTTTCATCCGCGCAGCGGTCGGGGCGAAAGCCGTGGGTGTATTTACGCCTTTGCCGAAACAGAAGTTGAAATCGGCATACCGGGCGGAGAACGCCATGCCAGCGTCGCTTTGCCCGGCGCAG  >  NC_000913/1073208‑1073400
                                                                                                 |                                                                                               
gtcgGTTTCATCGGCAATCACCATAAACAACACATAAGAGCCAACGTCGCGTCCGGTTTGCTCTGCGGCCTGTTTCATCCGCGCAGCGGTCGGGGCGaag                                                                                               >  2:1053169/1‑99 (MQ=255)
 tcgGTTTCATCGGCAATCACCATAAACAACACATAAGAGCCAACGTCGCGTCCGGTTTGCTCTGCGGCCTGTTTCATCCGCGCAGCGGTCGGGGCGaagc                                                                                              <  1:2875578/100‑3 (MQ=255)
 tcgGTTTCATCGGCAATCACCATAAACAACACATAAGAGCCAACGTCGCGTCCGGTTTGCTCTGCGGCCTGTTTCATCCGCGCAGCGGTCGGGGCGaagc                                                                                              <  1:208115/100‑3 (MQ=255)
 tcgGTTTCATCGGCAATCACCATAAACAACACATAAGAGCCAACGTCGCGTCCGGGTTGCTCTGCGGCCTGTTTCATCCGCGCAGCGGTCGGGGCGaagc                                                                                              <  1:752742/100‑3 (MQ=255)
    gTTTCATCGGCAATCACCATAAACAACACATAAGAGCCAACGTCGCGTCCGGTTTGCTCTGCGGCCTGTTTCATCCGCGCAGCGGTCGGGGCGAAGCCGt                                                                                          >  1:2794965/1‑100 (MQ=255)
    gTTTCATCGGCAATCACCATAAACAACACATAAGAGCCAACGTCGCGTCCGGTTTGCTCTGCGGCCTGTTTCATCCGCGCAGCGGTCGGGGCGAAGCCGt                                                                                          >  1:3048679/1‑100 (MQ=255)
    gTTTCATCGGCAATCACCATAAACAACACACAAGAGCCAACGTCGCGTCCGGTTTGCTCTGCGGCCTGTTTCATCCGCGCAGCGGTCGGGGCGAAGCCGt                                                                                          <  1:81628/100‑1 (MQ=255)
     tttCATCGGCAATCACCATAAACAACACATAAGAGCCAACGTCGCGTCCGGTTTGCTCTGCGGCCTGTTTCATCCGCGCAGCGGTCGGGGCGAAGCCGTg                                                                                         <  2:125401/100‑1 (MQ=255)
       tCATCGGCAATCACCATAAACAACACATAAGAGCCAACGTCGCGTCCGGTTTGCTCTGCGGCCTGTTTCATCCGCGCAGCGGTCGGGGCGAAGCCGTggg                                                                                       <  1:2990146/100‑1 (MQ=255)
       tCATCGGCAATCACCATAAACAACACATAAGAGCCAACGTCGCGTCCGGTTTGCTCTGCGGCCTGTTTCATCCGCGCAGCGGTCGGGGCGAAGCCGTggg                                                                                       <  1:1579829/100‑1 (MQ=255)
        cATCGGCAATCACCATAAACAACACATAAGAGCCAACGTCGCGTCCGGTTTGCTCTGCGGCCTGTTTCATCCGCGCAGCGGTCGGGGCGAAGCCGTGGgt                                                                                      <  2:2162450/100‑1 (MQ=255)
        cATCGGCAATCACCATAAACAACACATAAGAGCCAACGTCGCGTCCGGTTTGCTCTGCGGCCTGTTTCATCCGCGCAGCGGTCGGGGCGAAGCCGTGGgt                                                                                      <  2:122860/100‑1 (MQ=255)
         aTCGGCAATCACCATAAACAACACATAAGAGCCAACGTCGCGTCCGGTTTGCTCTGCGGCCTGTTTCATCCGCGCAGCGGTCGGGGCGAAGCCGTGGgtg                                                                                     >  1:2166040/1‑100 (MQ=255)
         aTCGGCAATCACCATAAACAACACATAAGAGCCAACGTCGCGTCCGGTTTGCTCTGCGGCCTGTTTCATCCGCGCAGCGGTCGGGGCGAAGCCGTGGgtg                                                                                     >  1:1972991/1‑100 (MQ=255)
         aTCGGCAATCACCATAAACAACACATAAGAGCCAACGTCGCGTCCGGTTTGCTCTGCGGCCTGTTTCATCCGCGCAGCGGTCGGGGCGAAGCCGTGGgtg                                                                                     >  2:1504509/1‑100 (MQ=255)
         aTCGGCAATCACCATAAACAACACATAAGAGCCAACGTCGCGTCCGGTTTGCTCGGCGGCCTGTTTCATCCGCGCAGCGGTCGGGGCGAAGCCGTGGgtg                                                                                     <  1:2486417/100‑1 (MQ=255)
          tCGGCAATCACCATAAACAACACATAAGAGCCAACGTCGCGTCCGGTTTGCTCTGCGGCCTGTTTCATCCGCGCAGCGGTCGGGGCGAAGCCGTGGgtgt                                                                                    >  2:2264080/1‑100 (MQ=255)
           cGGCAATCACCATATACAACACATAAGATCCATCGTCGCGTCCGGTTTGCGCTGCGGCCTGTTTCATCCGCGCAGCGGTCGGGGCGAAGCCGTGGgtgtn                                                                                   <  1:2407735/100‑2 (MQ=255)
           cGGCAATCACCATAAACAACACATAAGAGCCAACGTCGCGTCCGGTTTGCTCTGCGGCCTGTTTCATCCGCGCAGCGGTCGGGGCGAAGCCGTGGgtgt                                                                                    <  2:1330394/99‑1 (MQ=255)
           cGGCAATCACCATAAACAACACATAAGAGCCAACGTCGCGTCCGGTTTGCTCTGCGGCCTGTTTCATCCGCGCAGCGGTCGGGGCGAAGCCGTGGGTGTa                                                                                   <  2:2813540/100‑1 (MQ=255)
            ggCAATCACCATAAACAACACATAAGAGCCAACGTCGCGTCCGGTTTGCTCTGCGGCCTGTTTCATCCGCGCAGCGGTCGGGGCGAAGCCGTGGGTGTAt                                                                                  >  2:2622165/1‑100 (MQ=255)
             gCAATCACCATAAACAACACATAAGAGCCAACGTCGCGTCCGGTTTGCTCTGCGGCCTGTTTCATCCGCGCAGCGGTCGGGGCGAAGCCGTGGGTGTAtt                                                                                 >  2:2963413/1‑100 (MQ=255)
             gCAATCACCATAAACAACACATAAGAGCCAACGTCGCGTCCGGTTTGCTCTGCGGCCTGTTTCATCCGCGCAGCGGTCGGGGCGAAGCCGTGGGTGTAtt                                                                                 <  1:259001/100‑1 (MQ=255)
              cAATCACCATAAACAACACATAAGAGCCAACGTCGCGTCCGGTTTGCTCTGCGGCCTGTTTCATCCGCGCAGCGGTCGGGGCGAAGCCGTGGGTGTAttt                                                                                <  1:1745850/100‑1 (MQ=255)
              cAATCACCATAAACAACACATAAGAGCCAACGTCGCGTCCGGTTTGCTCTGCGGCCTGTTTCATCCGCGCAGCGGTCGGGGCGAAGCCGTGGGTGTAttt                                                                                >  1:1158441/1‑100 (MQ=255)
               aaTCACCATAAACAACACATAAGAGCCAACGTCGCGTCCGGTTTGCTCTGCGGCCTGTTTCATCCGCGCAGCGGTCGGGGCGAAGCCGTGGGTGTATTTa                                                                               <  2:625814/100‑1 (MQ=255)
                 tCACCATAAACAACACATATGAGCCAACGTCGCGTCCGGTTTGCTCTGCGGCCTGTTTCATCCGCGCAGCGGTCGGGGCGAAGCCGTGGGTGTATTTACg                                                                             <  1:2406795/100‑1 (MQ=255)
                 tCACCATAAACAACACATAAGAGCCAACGTCGCGTCCGGTTTGCTCTGCGGCCTGTTTCATCCGCGCAGCGGTCGGGGCGAAGCCGTGGGTGTATTTACg                                                                             >  2:2085285/1‑100 (MQ=255)
                  cACCATAAACAACACATAAGAGCCAACGTCGCGTCCGGTTTGCTCTGCGGCCTGTTTCATCCGCGCAGCGGTCGGGGCGAAGCCGTGGGTGTATTTACGc                                                                            <  2:675867/100‑1 (MQ=255)
                   actttAAACAACACATAAGATCCAACGTCGCTTCCGGTTTGCTCTGTTGCCTGTTTCATCCGCGCAGCGGTCGGGGCGAAGCCGTGGGTGTATTTACGcc                                                                           <  1:119929/96‑1 (MQ=255)
                   aCCATAAACAACACATAAGTTCCAACGTCGCGTTCGGTTTGCTCTGCGGCCTGTTTCATCCGCGCAGCGGTCGGGGCGAAGCCGTGGGTGTATTTACGcc                                                                           <  1:585288/100‑1 (MQ=255)
                   aCCATAAACAACACATAAGAGCCAACGTCGCGTCCGGTTTGCTCTGCGGCCTGTTTCATCCGCGCAGCGGTCGGGGCGAAGCCGTGGGTGTATTTACGcc                                                                           >  2:2280675/1‑100 (MQ=255)
                   aCCATAAACAACACATAAGAGCCAACGTCGCGTCCGGTTTGCTCTGCGGCCTGTTTCATCCGCGCAGCGGTCGGGGCGAAGCCGTGGGTGTATTTACGcc                                                                           >  1:1864267/1‑100 (MQ=255)
                     cATAAACAACACATAAGAGCCAACGTCGCGTCCGGTTTGCTCTGCGGCCTGTTTCATCCGCGCAGCGGTCGGGGCGAAGCCGTGGGTGTATTTACGCCtt                                                                         >  2:2295571/1‑100 (MQ=255)
                     cATAAACAACACATAAGAGCCAACGTCGCGTCCGGTTTGCTCTGCGGCCTGTTTCATCCGCGCAGCGGTCGGGGCGAAGCCGTGGGTGTATTTACGCCtt                                                                         >  1:2824257/1‑100 (MQ=255)
                     cATAAACAACACATAAGAGCCAACGTCGCGTCCGGTTTGCTCTGCGGCCTGTTTCATCCGCGCAGCGGTCGGGGCGAAGCCGTGGGTGTATTTACGCCtt                                                                         >  2:287249/1‑100 (MQ=255)
                     cATAAACAACACATAAGAGCCAACGTCGCGTCCGGTTTGCTCTGCGGCCTGTTTCATCCGCGCAGCGGTCGGGGCGAAGCCGTGGGTGTATTTACGCCtt                                                                         >  1:367759/1‑100 (MQ=255)
                       tAAACAACACATAAGAGCCAACGTCGCGTCCGGTTTGCTCTGCGGCCTGTTTCATCCGCGCAGCGGTCGGGGCGAAAGCCGTGGGTGTATTTACGCCttt                                                                        >  1:2227322/1‑100 (MQ=255)
                         aacaacACATAAGAGCCAACGTCGCGTCCGGTTTGCTCTGCGGCCTGTTTCATCCGCGCAGCGGTCGGGGCGAAGCCGTGGGTGTATTTACGCCTTTGcc                                                                     <  2:2940988/100‑1 (MQ=255)
                          acaacaCATAAGAGTTAACGTCGCGTTCGGTTTGCTCTGCGGCCTGTTTCATCCGCGCAGCGGTCCGGGCGAAGCCGTGGGTGTATTTACGCCTTTGCCg                                                                    >  2:2628274/1‑100 (MQ=255)
                          acaacaCATAAGAGCCAACGTCGCGTCCGGTTTGCTCTGCGGCCTGTTTCATCCGCGCAGCGGTCGGGGCGAAGCCGTGGGTGTATTTACGCCTTTGCCg                                                                    >  1:3018626/1‑100 (MQ=255)
                            aacaCATAAGAGCCAACGTCGCGTCCGGTTTGCTCTGCGGCCTGTTTCATCCGCGCAGCGGTCGGGGCGAAGCCGTGGGTGTATTTACGCCTTTGCCGaa                                                                  <  1:1923032/100‑1 (MQ=255)
                             acacATAAGAGCCAACGTCGCGTCCGGTTTGCTCTGCGGCCTGTTTCATCCGCGCAGCGGTCGGGGCGAAGCCGTGGGTGTATTTACGCCTTTGCCGaaa                                                                 >  1:2591769/1‑100 (MQ=255)
                              cacaTAAGTGCCAACGTCGCGTCCGGTTTGCTCTGCGGCCTGTTTCATCCGCGCAGCGGTCGGGGCGAAGCCGTGGGTGTATTTACGCCTTTGCCGAAAc                                                                <  1:1774695/100‑1 (MQ=255)
                              cacaTAAGAGCCAACGTCGCGTCCGGTTTGCTCTGCGGCCTGTTTCATCCGCGCAGCGGTCGGGGCGAAGCCGTGGGTGTATTTACGCCTTTGCCGAAAc                                                                <  2:1742729/100‑1 (MQ=255)
                                caTAAGAGCCAACGAAGCGACCGGAATGCTAAGCAGCCTGTTTCATACGCGCAGCGGTCGGGGCCAAGACGTGGGCGTATTTACGCCTTTGCCGAAACAg                                                              >  2:286067/1‑100 (MQ=255)
                                 aTAAGAGCCAACGTCGCGTCCGGTTTGCTCTGCGGCCTGTTTCATCCGCGCAGCGGTCGGGGCGAAGCCGTGGGTGTATTTACGCCTTTGCCGAAACAGa                                                             >  1:1551703/1‑100 (MQ=255)
                                  tAAGAGCCAACGTCGCGTCCGGTTTGCTCTGCGGCCTGTTTCATCCGCGCAGCGGTCGGGGCGAAGCCGTGGGTGTATTTACGCCTTTGCCGaaa                                                                 >  2:2082441/1‑95 (MQ=255)
                                  tAAGAGCCAACGTCGCGTCCGGTTTGCTCTGCGGCCTGTTTCATCCGCGCAGCGGTCGGGGCGAAGCCGTGGGTGTATTTACGCCTTTGCCGAAACAGaa                                                            <  1:576359/100‑1 (MQ=255)
                                   aaGAGCCAACGTCGCGTCCGGTTTGCTCTGCGGCCTGTTTCATCCGCGCAGCGGTCGGGGCGAAGCCGTGGGTGTATTTACGCCTTTGCCGAAACAGAAg                                                           <  1:2499030/100‑1 (MQ=255)
                                     gagCCAACGTCGCGTCCGGTTTGCTCTGCGGCCTGTTTCATCCGCGCAGCGGTCGGGGCGAAGCCGTGGGTGTATTTACGCCTTTGCCGAAACAGAAGtt                                                         <  1:1292015/100‑1 (MQ=255)
                                      agCCAACGTCGCGTCCGGTTTGCTCTGCGGCCTGTTTCATCCGCGCAGCGGTCGGGGCGAAGCCGTGGGTGTATTTACGCCTTTGCCGAAACAGAAGTTg                                                        >  2:1412443/1‑100 (MQ=255)
                                      agCCAACGTCGCGTCCGGTTTGCTCTGCGGCCTGTTTCATCCGCGCAGCGGTCGGGGCGAAGCCGTGGGTGTATTTACGCCTTTGCCGAAACAGAAGTTg                                                        <  1:1354770/100‑1 (MQ=255)
                                        ccAACGTCGCGTCCGGTTTGCTCTGCGGCCTGTTTCATCCGCGCAGCGGTCGGGGCGAAGCCGTGGGTGTATTTACGCCTTTGCCGAAACAGAAGTTGaa                                                      >  1:2808577/1‑100 (MQ=255)
                                          aaCGTCGCGTCCGGTTTGCTCTGCGGCCTGTTTCATCCGCGCAGCGGTCGGGGCGAAGCCGTGGGTGTATTTACGCCTTTGCCGAAACAGAAGTTGAAAt                                                    >  1:313906/1‑100 (MQ=255)
                                          aaCGTCGCGTCCGGTTTGCTCTGCGGCCTGTTTCATCCGCGCAGCGGTCGGGGCGAAGCCGTGGGTGTATTTACGCCTTTGCCGAAACAGAAGTTGAAAt                                                    >  1:318068/1‑100 (MQ=255)
                                             gTCGCGTCCGGTTTGCTCTGCGGCCTGTTTCATCCGCGCAGCGGTCGGGGCGAAGCCGTGGGTGTATTTACGCCTTTGCCGAAACAGAAGTTGAAATCgg                                                 <  2:2972039/100‑1 (MQ=255)
                                             gTCGCGTCCGGTTTGCTCTGCGGCCTGTTTCATCCGCGCAGCGGTCGGGGCGAAGCCGTGGGTGTATTTACGCCTTTGCCGAAACAGAAGTTGAAATCgg                                                 <  2:2825532/100‑1 (MQ=255)
                                             gTCGCGTCCGGTTTGCTCTGCGGCCTGTTTCATCCGCGCAGCGGTCGGGGCGAAGCCGTGGGTGTATTTACGCCTTTGCCGAAACAGAAGTTGAAATCgg                                                 <  1:1412457/100‑1 (MQ=255)
                                               cgcgTCCGGTTTGCTCTGCGGCCTGTTTCATCCGTGCAGCGGTCGGGGCGAAAGCCGTGGGTGTATTTACGCCTTTGCCGAAACAGAAGTTGAAATCGGc                                                <  1:1629753/100‑1 (MQ=255)
                                               cgcgTCCGGTTTGCTCTGCGGCCTGTTTCATCCGCGCAGCGGTCGGGGCGAAGCCGTGGGTGTATTTACGCCTTTGCCGAAACAGAAGTTGAAATCGGCa                                               <  2:2875141/100‑1 (MQ=255)
                                                gcgTCCGGTTTGCTCTGCGGCCTGTTTCATCCGCGCAGCGGTCGGGGCGAAGCCGTGGGTGTAGTTACGCCTTTGCCGAAACAGAAGTTGAAATCCGCAt                                              >  2:2580578/1‑100 (MQ=255)
                                                 cgTCCGGTTTGCTCTGCGGCCTGTTTCATCCGCGCAGCGGTCGGGGCGAAGCCGTGGGTGTATTTACGCCTTTGCCGAAACAGAAGTTGAAATCGGCATa                                             >  2:325506/1‑100 (MQ=255)
                                                 cgTCCGGTTTGCTCTGCGGCCTGTTTCATCCGCGCAGCGGTCGGGGCGAAGCCGTGGGTGTATTTACGCATTTGCCGAAACAGAAGTTGAAATCGGCATa                                             >  1:2458254/1‑100 (MQ=255)
                                                 cgTCCGGTTTGCTCTGCGGCCTGTTCCATCCTCGCAGCGTTCGGGACGAAGCCGTGGGTGTCTTTACGCCTTTGCCTAAACAGAAGTTGAAATCGGc                                                <  2:1321953/97‑1 (MQ=255)
                                                  gTCCGGTTTGCTCTGCGGCCTGTTTCATCCGCGCAGCGGTCGGGGCGAAGCCGTGGGTGTATTTACGCCTTTGCCGAAACAGAAGTTGAAATCGGCATAc                                            <  1:287252/100‑1 (MQ=255)
                                                    ccGGTTTGCTCTGCGGCCTGTTTCATCCGCGCAGCGGTCGGGGCGAAGCCGTGGGTGTATTTACGCCTTTGCCGAAACAGAAGTTGAAATCGGCATACCg                                          >  1:838056/1‑100 (MQ=255)
                                                     cGGTTTGCTCTGCGGCCTGTTTCATCCGCGCAGCGGTCGGGGCGAAGCCGTGGGTGTATTTACGCCTTTGCCGAAACAGAAGTTGAAATCGGCATACCgg                                         >  2:695484/1‑100 (MQ=255)
                                                       gTTTGCTCTGCGGCCTGTTTCATCCGCGCAGCGGTCGGGGCGAAGCCGTGGGTGTATTTACGCCTTTGCCGAAACAGAAGTTGAAATCGGCATACCGGGc                                       >  1:811677/1‑100 (MQ=255)
                                                         ttGCTCTGCGGCCTGTTTCATCCGCGCAGCGGTCGGGGCGAAGCCGTGGGTGTATTTACGCCTTTGCCGAAACAGAAGTTGAAATCGGCATACCGGGCgg                                     <  2:579152/100‑1 (MQ=255)
                                                         ttGCTCTGCGGCCTGTTTCATCCGCGCAGCGGTCGGGGCGAAGCCGTGGGTGTATTTACGCCTTTGCCGAAACAGAAGTTGAAATCGGCATACCGGGCgg                                     <  1:2684324/100‑1 (MQ=255)
                                                          tGCTCTGCGGCCTGTTTCATCCGCGCAGCGGTCGGGGCGAAAGCCGTGGGTGTATTTACGCCTTTGCCGAAACAGAAGTTGAAATCGGCATACCGGGCgg                                     >  2:1336259/1‑100 (MQ=255)
                                                          tGCTCTGCGGCCTGTTTCATCCGCGCAGCGGTCGGGGCGAAGCCGTGGGTGTATTTACGCCTTTGCCGAAACAGAAGTTGAAATCGGCATACCGGGCGga                                    >  2:2412141/1‑100 (MQ=255)
                                                           gCTCTGCGGCCTGTTTCATCCGCGCAGCGGTCGGGGCGAAGCCGTGGGTGTATTTACGCCTTTGCCGAAACAGAAGTTGAAATCGGCATACCGGGCGgag                                   >  2:2517975/1‑100 (MQ=255)
                                                            ctctGCGGCCTGTTTCATCCGCGCAGCGGTCGGGGCGAAGCCGTGGGTGTATTTACGCCTTTGCCGAAACAGAAGTTGAAATCGGCATACCGGGCGgaga                                  >  1:1519626/1‑100 (MQ=255)
                                                             tctGCGGCCTGTTTCATCCGCGCAGCGGTCGGGGCGAAGCCGTGGGTGTATTTACGCCTTTGCCGAAACAGAAGTTGAAATCGGCATACCGGGCGGAGaa                                 >  1:2471370/1‑100 (MQ=255)
                                                              ctGCGGCCTGTTTCATCCGCGCAGCGGTCGGGGCGAAGCCGTGGGTGTATTTACGCCTTTGCCGAAACAGAAGTTGAAATCGGCATACCGGGAGGAGAAc                                <  1:1589988/100‑1 (MQ=255)
                                                                    ccTGTTTCATCCGCGCAGCGGTCGGGGCGAAGCCGTGGGTGTATTTACGCCTTTGCCGAAACAGAAGTTGAAATCGGCATACCGGGCGGAGAACGCCATg                          <  1:695488/100‑1 (MQ=255)
                                                                      tGTTTCATCCGCGCAGCGGTCGGGGCGAAGCCGTGGGTGTATTTACGCCTTTGCCGAAACAGAAGTTGAAATCGGCATACCGGGCGGAGAACGCCATGcc                        <  1:2306319/100‑1 (MQ=255)
                                                                       gTTTCATCCGCGCAGCGGTCGGGGCGAAGCCGTGGGTGTATTTACGCCTTTGCCGAAACAGAAGTTGAAATCGGCATACCGGGCGGAGAACGCCATGcc                        >  1:506485/1‑99 (MQ=255)
                                                                          tCATCCGCGCAGCGGTCGGGGCGAAGCCGTGGGTGTATTTACGCCTTTGCCGAAACAGAAGTTGAAATCGGCATACCGGGCGGAGAACGCCATGCCAGCg                    <  1:1328525/100‑1 (MQ=255)
                                                                          tCATCCGCGCAGCGGTCGGGGCGAAGCCGTGGGTGTATTTACGCCTTTGCCGAAACAGAAGTTGAAATCGGCATACCGGGCGGAGAACGCCATGCCAGCg                    <  1:466959/100‑1 (MQ=255)
                                                                          tCATCCGCGCAGCGGTCGGGGCGAAGCCGTGGGTGTATTTACGCCTTTGCCGAAACAGAAGTTGAAATCGGCATACCGGGCGGAGAACGCCATGCCAGCg                    <  2:2794933/100‑1 (MQ=255)
                                                                           cATCCGCGCAGCGGTCGGGGCGAAGCCGTGGGTGTATTTACGCCTTTGCCGAAACAGAAGTTGAAATCGGCATACCGGGCGGAGAACGCCATGCCAGCGt                   >  2:2262275/1‑100 (MQ=255)
                                                                           cATCCGCGCAGCGGTCGGGGCGAAGCCGTGGGTGTATTTACGCCTTTGCCGAAACAGAAGTTGAAATCGGCATACCGGGCGGAGAACGCCATGCCAGCGt                   >  2:1187763/1‑100 (MQ=255)
                                                                            aTCCGCGCAGCGGTCGGGGCGAAGCCGTGGGTGTATTTACGCCTTTGCCGAAACAGAAGTTGAAATCGGCATACCGGGCGGAGAACGCCATGCCAGCGTc                  >  2:1319749/1‑100 (MQ=255)
                                                                            aTCCGCGCAGCGGTCGGGGCGAAGCCGTGGGTGTATTTACGCCTTTGCCGAAACAGAAGTTGAAATCGGCATACCGGGCGGAGAACGCCATGCCAGCGTc                  <  1:2264105/100‑1 (MQ=255)
                                                                            aTCCGCGCAGCGGTCGGGGCGAAGCCGTGGGTGTATTTACGCCTTTGCCGAAACAGAAGTTGAAATCGACATACCGGGCGGAGAACGCCATGCCAGCGTc                  >  1:1289052/1‑100 (MQ=255)
                                                                             tCCGCGCAGCGGTCGGGGCGAAGCCGTGGGTGTATTTACGCCTTTGCCGAAACAGAAGTTGAAATCGGCATACCGGGCGGAGAACGCCATGCCAGCGTCg                 <  2:782314/100‑1 (MQ=255)
                                                                                  gcAGCGGTCGGGGCGAAGCCGTGGGTGTATTTACGCCTTTGCCGAAACAGAAGTTGAAATCGGCATACCGGGCGGAGAACGCCATGCCAGCGTCGCTTTg            <  1:2232184/100‑1 (MQ=255)
                                                                                   cAGCGGTCGGGGCGAAGCCGTGGGTGTATTTACGCCTTTGCCGAAACAGAAGTTGAAATCGGCATACCGGGCGGAGAACGCCATGCCAGCGTCGCTTTGc           <  2:2157272/100‑1 (MQ=255)
                                                                                    aGCGGTCGGGGCGCAGCCGTGGGTGTATTTACGCCTTTGCCGAAACAGAAGTTGAAATCGGCATACCGGGCGGAGAACGCCATGCCAGCGTCGCTTTGcc          >  2:3018044/1‑100 (MQ=255)
                                                                                    aGCGGTCGGGGCGAAGCCGTGGGTGTATTTACGCCTTTGCCGAAACAGAAGTTGAAATCGGCATACCGGGCGGAGAACGCTATGCCAGCGTCGCTTTGcg          <  2:1341845/100‑2 (MQ=255)
                                                                                    aGCGGTCGGGGCGAAGCCGTGGGTGTATTTACGCCTTTGCCGAAACAGAAGTTGAAATCAGCATACCGGGCGGAGAACGCCATGCCAGCGTCGCTTTGcc          >  1:2701939/1‑100 (MQ=255)
                                                                                      cGGTCGGGGCGAAGCCGTGGGTGTATTTACGCCTTTGCCGAAACAGAAGTTGAAATCGGCATACCGGGCGGAGAACGCCATGCCAGCGTCGCTTTGCCCg        <  2:1474067/100‑1 (MQ=255)
                                                                                        gTCGGGGCGAAGCCGTGGGTGTATTTACGCCTTTGCCGAAACAGAAGTTGAAATCGGCATACCGGGCGGAGAACGCCATGCCAGCGTCGCTTTGCCCgg       <  2:2591737/99‑1 (MQ=255)
                                                                                        gTCGGGGCGAAGCCGTGGGTGTATTTACGCCTTTGCCGAAACAGAAGTTGAAATCGGCATACCGGGCGGAGAACACCATGCCAGCGTCGCTTTGCCCGgc      >  2:509793/1‑100 (MQ=255)
                                                                                          cGGGGCGAAGCCGTGGGTGTATTTACGCCTTTGCCGAAACAGAAGTTGAAATCGGCATACCGGGCGGAGAACGCCATGCCAGCGTCGCTTTGCCCGgcgc    >  1:654699/1‑100 (MQ=255)
                                                                                          cGGGGCGAAGCCGTGGGTGTATTTACGCCTTTGCCGAAACAGAAGTTGAAATCGGCATACCGGGCGGAGAACGCCATGCCAGCGTCGCTTTGCCCGgcgc    <  2:2399055/100‑1 (MQ=255)
                                                                                            gggCGAAGCCGTGGGTGTATTTACGCCTTTGCCGAAACAGAAGTTGAAATCGGCATACCGGGCGGAGAACGCCATGCCAGCGTCGCTTTGCCCGGCGCAg  >  1:2417131/1‑100 (MQ=255)
                                                                                                 |                                                                                               
GTCGGTTTCATCGGCAATCACCATAAACAACACATAAGAGCCAACGTCGCGTCCGGTTTGCTCTGCGGCCTGTTTCATCCGCGCAGCGGTCGGGGCGAAAGCCGTGGGTGTATTTACGCCTTTGCCGAAACAGAAGTTGAAATCGGCATACCGGGCGGAGAACGCCATGCCAGCGTCGCTTTGCCCGGCGCAG  >  NC_000913/1073208‑1073400

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: