Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,170,338 (A)6→5 100% coding (37/963 nt) ldtC ← L,D‑transpeptidase linking Lpp to murein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,170,3330A.95.7% 490.7 / 11.1 115coding (42/963 nt)ldtCL,D‑transpeptidase linking Lpp to murein
Reads supporting (aligned to +/- strand):  ref base A (1/3);  new base . (47/63);  total (49/66)
Fisher's exact test for biased strand distribution p-value = 6.37e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.79e-01

Only 100 of 127 total aligned reads displayed.

ATGAAATTTGTTTTCGCCAACCAGACGACTGCCCGCTGGCGGCAGCGGCCAGGTGTTGGCTTTTGCCGGTAGCGCCAGCGCCACGGCAGCGGCGAACGTAAAAAACGTTAGCCAGCGAGAAAAACGCGTTTTGATCATCACCAAAAATCCATAATAAATATAAGGTTATTGTAATAAACAGATAAGCCTGAATTA  >  NC_000913/1170234‑1170428
                                                                                                   |                                                                                               
aTGAAATTTGTTTTCGCCAACCAGACGACTGCCCGCTGGCGGCAGCGGCCAGGTGTTGGCTTTTGCCGGTAGCGCCAGCGCCACGGCAGCGGCGAACGTa                                                                                                 <  1:1744890/100‑1 (MQ=255)
aTGAAATTTGTTTTCGCCAACCAGACGACTGCCCGCTGGCGGCAGCGGCCAGGTGTTGGCTTTTGCCGGTAGCGCCAGCGCCACGGCAGCGGCGAACGTa                                                                                                 >  2:604023/1‑100 (MQ=255)
   aaaTTTGTTTTCGCCAACCAGACGACTGCCCGCTGGCGGCAGCGGCCAGGTGTTGGCTTTTGCCGGTAGCGCTAGCGCCACGGCAGCGGCGAACGTaaaa                                                                                              <  2:2277941/100‑1 (MQ=255)
   aaaTTTGTTTTCGCCAACCAGACGACTGCCCGCTGGCGGCAGCGGCCAGGTGTTGGCTTTTGCCGGTAGCGCCAGCGCCACGGCAGCGGCGAACGTaaaa                                                                                              >  2:2144043/1‑100 (MQ=255)
    aaTTTGTTTTCGCCAACCAGACGACTGCCCGCTGGCGGCAGCGGCCAGGTGTTGGCTTTTGCCGGTAGCGCCAGCGCCACGGCAGCGGCGAACGTaaaaa                                                                                             >  1:1726622/1‑100 (MQ=255)
      tttGTTTTCGCCAACCAGACGACTGCCCGCTGGCGGCAGCGGCCAGGTGTTGGCTTTTGCCGGTAGCGCCAGCGCCACGGCAGCGGCGAACGTaaaaacg                                                                                           <  1:1710106/100‑3 (MQ=255)
        tGTTTTCGCCAACCAGACGACTGCCCGCTGGCGGCAGCGGCCAGGTGTTGGCTTTTGCCGGTAGCGCCAGCGCCACGGCAGCGGCGAACGTaaaaacgtt                                                                                         <  1:91790/100‑5 (MQ=255)
        tGTTTTCGCCAACCAGACGACTGCCCGCTGGCGGCAGCGGCCAGGTGTTGGCTTTTGCCGGTAGCGCCAGCGCCACGGCAGCGGCGAACGTaaaaacgtt                                                                                         <  2:1467312/100‑5 (MQ=255)
         gTTTTCGCCAACCAGACGACTGCCCGCTGGCGGCAGCGGCCAGGTGTTGGCTTTTGCCGGTAGCGCCAGCGCCACGGCAGCGGCGAACGTAAAAACGTTa                                                                                       <  2:376375/100‑1 (MQ=255)
          ttttCGCCAACCAGACGACTTCCCGCTGGCGGCAGCGGCCAGGTGTTGGCTTTTGCCGGTAGCGCCAGCGCCACGGCAGCGGCGAACGTAAAAACGTTAg                                                                                      <  1:714407/100‑1 (MQ=255)
          ttttCGCCAACCAGACGACTGCCCGCTGGCGGCAGCGGCCAGGTGTTGGCTTTTGCCGGTAGCGCCAGCGCCACGGCAGCGGCGAACGTAAAAACGTTAg                                                                                      <  2:2108879/100‑1 (MQ=255)
          ttttCGCCAACCAGACGACTGCCCGCTGGCGGCAGCGGCCAGGTGTTGGCTTTTGCCGGTAGCGCCAGCGCCACGGCAGCGGCGAACGTAAAAACGTTAg                                                                                      >  2:2832248/1‑100 (MQ=255)
            ttCTCCAACCAGACGACTGCCCGCTGGCGGCAGCGGCCAGGTGTTGGCTTTTGCCGGTAGCGCCAGCGCCACGGCAGCGGCGAACGTAAAAACGTTAg                                                                                      <  2:193524/98‑1 (MQ=255)
            ttCGCCACCGCGACGACTGCCCGCTGGAGGCAGAGGCCAGGTGTTGGCTTTTGCCGGTAGCGCCAGCGCCACGGCAGCGGCGAACGTAAAAACGTTAGc                                                                                     <  2:1764834/99‑1 (MQ=255)
            ttCGCCAACCAGACGACTGCCCGCTGGCGGCAGCGGCCAGGTGTTGGCTTTTGCCGGTAGCGCCAGCGCCACGGCAGCGGCGAACGTAAAAACGTTAGcc                                                                                    >  1:1447755/1‑100 (MQ=255)
             tCGCCAACCAGACGACTGCCCGCTGGCGGCAGCGGCCAGGTGTTGGCTTTTGCCGGTAGCGCCAGCGCCACGGCAGCGGCGAACGTAAAAACGTTAGCCa                                                                                   >  1:2377899/1‑100 (MQ=255)
             tCGCCAACCAGACGACTGCCCGCTGGCGGCAGCGGCCAGGTGTTGGCTTTTGCCGGTAGCGCCAGCGACAAGGCAGCGGCGAACGTAAAAACGTTAGcag                                                                                   >  1:1764854/1‑98 (MQ=255)
              cGCCAACCAGACGACTGCCCGCTGGCGGCAGCGGCCAGGTGTTGGCTTTTGCCGGTAGCGCCAGCGCCACGGCAGCGGCGAACGTAAAAACGCTAGCCAg                                                                                  <  1:1585426/100‑1 (MQ=255)
               gCCAACCAGACGACTGCCCGCTGGCGGCAGCGGCCAGGTGTTGGCTTTTGCCGGTAGCGCCAGCGCCACGGCAGCGGCGAACATAAAAACGTTAGCCAGc                                                                                 <  1:2617559/100‑1 (MQ=255)
                ccAACCAGACGACTGCCCGCTGGCGGCAGCGGCCAGGTGTTGGCTTTTGCCGGTAGCGCCAGCGCCACGGCAGCGGCGAACGTAAAAACGTTAGCCAGCg                                                                                <  2:3004169/100‑1 (MQ=255)
                   aCCAGACGACTGCCCGCTGGCGGCAGCGGCCAGGTGTTGGCTTTTGCCGGTAGCGCCAGCGCCACGGCAGCGGCGAACGTAAAAAACGTTAGCCAGCgag                                                                              <  1:1971161/100‑1 (MQ=255)
                   aCCAGACGACTGCCCGCTGGCGGCAGCGGCCAGGTGTTGGCTTTTGCCGGTAGCGCCAGCGCCACGGCAGCGGCGAACGTAAAAACGTTAGCCAGCgaga                                                                             <  1:2870550/100‑1 (MQ=255)
                    ccAGACGACTGCCCGCTGGCGGCAGCGGCCAGGTGTTGGCTTTTGCCGGTAGCGCCAGCGCCACGGCAGCGGCGAACGTAAAAACGTTAGCCAGCGAGaa                                                                            <  1:2976202/100‑1 (MQ=255)
                     cAGACGACTGCCCGCTAACACCAACGGCCAGATGATGTCTCTTGCCGGTACCCCCACAGCCACGACCGCCGCGAACGTAAACACCTTAACCACCGAGaa                                                                            >  2:823841/1‑99 (MQ=255)
                      aGACGACTGCCCGCTGGCGGCAGCGGCCAGGTGTTGGCTTTTGCCGGTAGCGCAAGCGCCACGGCAGCGGCGAACGTAAAAACGTTAGCCAACGAGaaaa                                                                          >  1:2542719/1‑100 (MQ=255)
                      aGACGACTGCCCGCTCGCGGCAGCGGCCAGGTGTTGGCTTTTGCCGGTAGCGCCAGCGCCACGGCAGCGGCGAACGGAAAAACGTTAGCCAGCGAGgaaa                                                                          >  2:556236/1‑100 (MQ=255)
                         cgacTGCCCGCTGGCGGCAGCGGCCAGGTGTTGGCTTTTGCCGGTAGCGCCAGCGCCACGGCAGCGGCGAACGTAAAAACGTTAGCCAGCGAGAAAAAcg                                                                       <  1:604027/100‑1 (MQ=255)
                          acctggccGCTGCCGCCAGCGGGCAGGTGTTGGCTTTTGCCGGTAGCGCCAGCGCCACGGCAGCGGCGAACGTAAAAACGTTAGCCAGCGAGAAAAAcgc                                                                      >  1:856668/7‑100 (MQ=37)
                           ccTGGCCGCTGCCGCCAGCGGGCAGGTGTTGGCTTTTGCCTGTAGCGCCAGCGCCACGTCAGCGGCGAACGTAAAAACGTTAGCCAGCGAGAAAAAcg                                                                       >  1:562805/2‑98 (MQ=37)
                           ccTGGCCGCTGCCGCCAGCGGGCAGGTGTTGGCTTTTGCCGGTAGCGCCAGCGCCACGGCAGCGGCGAACGTAAAAACGTTAGCCAGCGAGAAAAAcgcg                                                                     >  2:2241222/2‑100 (MQ=255)
                           ccTGGCCGCTGCCGCCAGCGGGCAGGTGTTGGCTTTTGCCGGTAGCGCCAGCGCCACGGCAGCGGCGAACGTAAAAACGTTAGCCAGCGAGAAAAAcgcg                                                                     >  1:2763132/2‑100 (MQ=255)
                           ccTGGCCGCTGCCGCCAGCGAGCAGGTGTTGGCTTTTGCCGGTAGCGCCAGCGCCACGGCAGCGGCGTACGTAAAAACGTTAGCCAGCGAGAAAAAcgcg                                                                     >  2:2131295/2‑100 (MQ=255)
                            cTGGCCGCTGCCGCCAGCGGGCAGGTGTTGGCTTTTGCCGGTAGCGCCAGCGCCACGGCAGCGGCGAACGTAAAAACGTTAGCCAGCGAGAAAAACGCGt                                                                    >  2:2996570/1‑100 (MQ=255)
                            cTGCCCGCTGGCGGCAGCGGCCAGGTGTTGGCTTTTGCCGGTAGCGCCAGCGCCACGGCAGCGGCGAACGTAAAAACGTTAGCCAGCGAGAAAAACGCGt                                                                    <  1:226855/100‑1 (MQ=255)
                             tggccGCTGCCGCCAGCGGGCAGGTGTTGGCTTTTGCCGGTAGCGCCAGCGCCACGGCAGCGGCGAACGTAAAAACGTTAGCCAGCGAGAAAAACGCGtt                                                                   >  1:1875293/4‑100 (MQ=255)
                             tggccGCTGCCGCCAGCGGGCAGGTGTTGGCTCTTGCCGGTAGCGCCAGCGCCACCGCAGCGGAGAACGTAAAAACATTAGCCAGCGAGAAAAACGCGt                                                                    >  1:1281554/4‑99 (MQ=255)
                             tGCCCGCTGGCGGCAGCGGCCAGGTGTTGGCTTTTGCCGGTAGCGCCAGCGCCACGGCAGCGGCGAACGTAAAAACGTTAGCCAGCGAGAAAAACGCGtt                                                                   >  1:1687595/1‑100 (MQ=255)
                             tGCCCGCTGGCGGCAGCGGCCAGGTGTTGGCTTTTGCCGGTAGCGCCAGCGCCACGGCAGCGGCGAACGTAAAAACGTTAGCCAGCGAGAAAAACGCGtt                                                                   <  1:1192841/100‑1 (MQ=255)
                              gCCCGCTGGCGGCAGCGGCCAGGTGTTGGCTTTTGCCGGTAGCGCCAGCGCCACGGCAGCGGCGAACGTAAAAACGTTAGCCAGCGAGAAAAACGCGttt                                                                  >  2:1110977/1‑100 (MQ=255)
                              gCCCGCTGGCGGCAGCGGCCAGGTGTTGGCTTTTGCCGGTAGCGCCAGCGCCACGGCAGCGGCGAACGTAAAAACGTTAGCCAGCGAGAAAAACGCGttt                                                                  >  1:1017572/1‑100 (MQ=255)
                               cccGCTGGCGGCAGCGGCCAGGTGTTGGCTTTTGCCGGTAGCGCCAGCGCCACGGCAGCGGCGAACGTAAAAACGTTAGCCAGCGAGAAAAACGCGtttt                                                                 >  1:2865599/1‑100 (MQ=255)
                                    tggtggCAGCGGCCAGGTGTTGGCTTTTGCCGGTAGCGCCAGCGCCACGGCAGCGGCGAACGTAAAAACGTTAGCCAGCGAGAAAAACGCGTTTTGatca                                                            <  1:3036438/100‑1 (MQ=255)
                                      gcggcAGCGGCCAGGTGTTGGCTTTTGCCGGTAGCGCCAGCGCCACGGCAGCGGCGAACGTAAAAACGTTAGCCAGCGAGAAAAACGCGTTTTGatcatc                                                          >  2:1243744/1‑100 (MQ=255)
                                      gcggcAGCGGCCAGGTGTTGGCTTTTGCCGGTAGCGCCAGCGCCACGGCAGCGGCGAACGTAAAAACGTTAGCCAGCGAGAAAAACGCGTTTTGatcatc                                                          <  1:1110987/100‑1 (MQ=255)
                                      gcggcAGCGGCCAGGTGTTGGCTTTTGCCGGTAGCGCCAGCGCCACGGCAGCGGCGAACGTAAAAACGTTAGCCAGCGAGAAAAACGCGTTTTGatcatc                                                          <  1:2096371/100‑1 (MQ=255)
                                       cggcAGCGGCCAGGTGTTGGCTTTTGCCGGTAGCGTCAGCGCCACGGCAGCGGCGAACGTAAAAACGTTAGCCAGCGAGAAAAACGCGTTTTGAtcatca                                                         <  1:940715/100‑1 (MQ=255)
                                       cggcAGCGGCCAGGTGTTGGCTTTTGCCGGTAGCGCCAGCGCCACGGCAGCGGCGAACGTAAAAACGTTAGCCAGCGAGAAAAACGCGTTTTGAtcatca                                                         <  2:27092/100‑1 (MQ=255)
                                       cggcAGCGGCAAGGTGTTGGCTTTTGCCGGTAGCGCCAGCGCCACGGCAGCGGCGAACGTAAAAACGTTAGCCAGCGAGAAAAACGCGTTTTGatcatc                                                          >  2:570158/1‑99 (MQ=255)
                                        ggcAGCGGCCAGGTGTTGGCTTTTGCCGGTAGCGCCAGCTCCACGGCAGCGGCGAACGTAAAAACGTTAGGCAGCGAGAAAAACGAGTTTTGATCATCAc                                                        >  2:2852240/1‑100 (MQ=255)
                                        ggcAGCGGCCAGGTGTTGGCTTTTGCCGGTAGCGCCAGCGCCACGGCAGCGGCGAACGTAAAAACGTTAGCCAGCGAGAAAAACGCGTTTTGATCATCAc                                                        <  1:1922746/100‑1 (MQ=255)
                                         gtaGCGGCCAGGTGTTGGCTTTTGTCTGTAGCGCCAGCGTCACGGCAGCGGTGAACGTAAAAATTTTAGCCAGCGAGAAAAACGCGTTTTGAtcatca                                                         <  2:1408000/96‑1 (MQ=255)
                                          cAGCGGCCAGGTGTTGGCTTTTGCCGGTAGCGCCAGCGCCACGGCAGCGGCGAACGTAAAAAACGTTAGCCAGCGAGAAAAACGCGTTTTGATCATCAcc                                                       <  1:2769374/100‑1 (MQ=255)
                                          cAGCGGCCAGGTGTTGGCTTTTGCCGGTAGCGCCAGCGCCACGGCAGCGGCGAACGTAAAAACGTTAGCCAGCGAGAAAAACGCGTTTTGATCATCACCa                                                      >  2:2026637/1‑100 (MQ=255)
                                          cAGCGGCCAGGTGTTGGCTTTTGCCGGTAGCGCCAGCGCCACGGCAGCGGCGAACGTAAAAACGTTAGCCAGCGAGAAAAACGCGTTTTGATCATCACCa                                                      >  2:2605228/1‑100 (MQ=255)
                                             cGGCCAGGTGTTGGCTTTTGCCGGTAGCGCCAGCGCCACGGCAGCGGCGAACGTAAAAACGTTAGCCAGCGAGAAAAACGCGTTTTGATCATCACCaaaa                                                   >  2:2099199/1‑100 (MQ=255)
                                             cGGCCAGGTGTTGGCTTTTGCCGGTAGCGCCAGCGCCACGGCAGCGGCGAACGTAAAAACGTTAGCCAGCGAGAAAAACGCGTTTTGATCATCACCaaa                                                    >  2:2410374/1‑99 (MQ=255)
                                                  aGGTGTTGGCTTTTGCCGGTAGCGCCAGCGCCACGGCAGCGGCGAACGTAAAAACGTTAGCCAGCGAGAAAAACGCGTTTTGATCATCACCAAAAATCCa                                              <  2:1163388/100‑1 (MQ=255)
                                                   ggtggtGGCTTTTGCCGGTCGCGCCAGCGCCACGGCAGCGGCGAACGTAAAAACGTTAGCCAGCGAGAAAAACGCGTTTTGTTCATCACCAAAAATcc                                               <  2:2731824/98‑1 (MQ=255)
                                                   ggTGTTGGCTTTTGCCTGTAGCGCCAGCTCCACGGCAGCGGCGAACGTTTTTACGTTATCCAGCGATAAAAACGCGTTTTGATCATCAcc                                                       <  2:2865619/90‑1 (MQ=255)
                                                   ggTGTTGGCTTTTGCCGGTAGCGCCAGCGCCATGGCAGCCGCCAACGTAAAAACGTTAGCCAGCGAGAAAAACGCGTTTTGATCATCACCAAAAATCCat                                             <  2:2132101/100‑1 (MQ=255)
                                                   ggTGTTGGCTTTTGCCGGTAGCGCCAGCGCCACGGCAGCGGCGAACGTAAAAACGTTAGCCAGCGAGAAAAACGCGTTTTGATCATCACCAAAAATCCat                                             <  2:166855/100‑1 (MQ=255)
                                                   ggTGTTGGCTTTTGCCGGTAGCGCCAGCGCCACGGCAGCGGCGAACGTAAAAACGTTAGCCAGCGAGAAAAACGCGTTTTGATCATCACCAAAAATCCat                                             <  2:817167/100‑1 (MQ=255)
                                                   ggTGTTGGCTTTTGCCGGTAGCGCCAGCGCCACGGCAGCGGCGAACGTAAAAACGTTAGCCAGCGAGAAAAACGCGTTTTGATCATCACCAAAAATCCat                                             >  1:415101/1‑100 (MQ=255)
                                                   ggTGTTGGCTTTTGCCGGTAGCGCCAGCGCCACGGCAGCGGCGAACGTAAAAACGTTAGCCAGCGAGAAAAACGCGTTTTGATCATCACCAAAAATCCat                                             <  2:177154/100‑1 (MQ=255)
                                                      gtTGGCTTTTGCCGGTATCGCCAGCGCCACGGCAGCGGCGAACGTAAAAACGTTAGCCAGCGAGAAAAACGCGTTTTGATCATCACCAAAAATCCataat                                          <  2:2547592/100‑1 (MQ=255)
                                                      gtTGGCTTTTGCCGGTAGCGCCAGCGCCACGGCAGCGGCGAACGTAAAAACGTTAGCCAGCGAGAAAAACGCGTTTTGATCATCACCAAAAATCCataat                                          >  1:2547623/1‑100 (MQ=255)
                                                         ggCTTTTGCCGGTAGCGCCAGCGCCACGGCAGCGGCGAACGTAAAAACGTTAGCCAGCGAGAAAAACGCGTTTTGATCATCACCAAAAATCCATaataaa                                       >  2:2280664/1‑100 (MQ=255)
                                                          gCTTTTGCCGGTAGCGCCAGTGCCACGGCAGCGGCGAACGTAAAAACGTTAGCCAGCGAGAAAAACGCGTTTTGATCATCACCAAAAATCCATaataaat                                      <  1:158883/100‑1 (MQ=255)
                                                           cTTTTGCCGGTAGCGCCAGCGCCACGGCAGCGGCGAACGTAAAAAACGTTAGCCAGCGAGAAAAACGCGTTTTGATCATCACCAAAAATCCATaataaat                                      <  1:2927073/100‑1 (MQ=255)
                                                            ttttGCCGGTAGCGCCAGCGCCACGGCAGCGGCGAACGTAAAAACGTTAGCCAGCGAGAAAAACGCGTTTTGATCATCACCAAAAATCCATAATAAatat                                    >  1:2341989/1‑100 (MQ=255)
                                                             tttGCCGGTAGCGCCAGCGCCACGGCAGCGGCGAACGTAAAAACGTTAGCCAGCGAGAAAAACGCGTTTTGATCATCACCAAAAATCCATAATAAAtata                                   <  1:1988790/100‑1 (MQ=255)
                                                               tGCCGGTAGCGCCAGCGTCACGGCAGCGGCGATCGTAAAAACGTTAGCCAGCGAGAAAAACGCGTTTTGATCATCACCAAAAATCCATAATAAATATAAg                                 <  1:2639984/100‑1 (MQ=255)
                                                               tGCCGGTAGCGCCAGCGCCACGGCAGCGGCGAACGTAAAAACGTTAGCCAGCTAGAAAAACGCGTTTTTATCATCACTAAAAATCCATAATAAATATAAg                                 <  2:519475/100‑1 (MQ=255)
                                                                 ccGGTAGCGCCAGCGCCACGGCAGCGGCGAACGTAAAAACGTTAGCCAGCGAGAAAAACGCGTTTTGATCATCACCAAAAATCCATAATAAATATAAGGt                               <  1:1977721/100‑1 (MQ=255)
                                                                   ggTAGCGCCAGCGCCACGGCAGCGGCGAACGTAAAAACGTTAGCCAGCGAGAAAAACGCGTTTTGATCATCACCAAAAATCCATAATAAATATAAGGTTa                             >  2:1385058/1‑100 (MQ=255)
                                                                   ggTAGCGCCAGCGCCACGGCAGCGGCGAACGTAAAAACGTTAGCCAGCGAGAAAAACGCGTTTTGATCATCACCAAAAATCCATAATAAATATAAGGTTa                             >  1:1854564/1‑100 (MQ=255)
                                                                    gTAGCGCCAGCGCCACGGCAGCGGCGAACGTAAAAACGTTAGCCAGCGAGAAAAACGCGTTTTGATCATCACCAAAAATCCATAATAAATATAAGGTTAt                            >  1:2498165/1‑100 (MQ=255)
                                                                    gTAGCGACAGCGCCACGGCAGCGGCGAACGTAAAAACGTTAGCCAGCGAGAAATACGCGTTTTGATCATCACCAAAAATCCATAATAAATATAAg                                 <  2:1002941/95‑1 (MQ=255)
                                                                      agcgccagcgccACGGCAGCGGCGAACGTAAAAACGTTAGCCAGCTAGAAAAACGCGTTTTTATCATCACCTAAAATCCTTAATAAATATAAGGTTAtt                           <  2:1238406/99‑1 (MQ=255)
                                                                      agcgccagcgccACGGCAGCGGCGAACGTAAAAACGTTAGCCAGCGAGAAAAACGCGTTTTGATCATCACCAAAAATCCATAATAAATATAAGGTTATTg                          <  2:152708/100‑1 (MQ=255)
                                                                       gcgaaagcgccaCGGCAGCGGCGAACGTAAAAACGTTAGCCAGCGAGAAAAACGCGTTTTGATCATAACTAAAAATCCATAATAAATATAAGGTTATTg                          <  2:2005474/94‑1 (MQ=255)
                                                                        cgccagcgccaCGGCAGCGGCGAACGTAAAAACGTTAGCCAGTGAGAAAAACGCGTTTTGATCATCACCAAAAATCCATAATAAATATAAGGTTATTGta                        <  1:45582/100‑1 (MQ=255)
                                                                        cgccagcgccaCGGCAGCGGCGAACGTAAAAACGTTAGCCAGCGAGAAAAACGCGTTTTGATCATCACCAAAAATCCATAATAAATATAAGGTTATTGta                        >  1:2728387/1‑100 (MQ=255)
                                                                        cgccagcgccaCGGCAGCGGCGAACGTAAAAACGTTAGCCAGCGAGAAAAACGCGAAATGATCATCACCAAAAATCCATAATAAATATAAGGTTATTGta                        >  1:801667/1‑100 (MQ=255)
                                                                          ccagcgccaCGGCAGCGGCGAACGTAAAAACGTTAGCCAGCGAGAAAAACGCGTTTTGATCATCACCAAAAATCCATAATAAATATAAGGTTATTGtaat                      <  2:1813709/100‑1 (MQ=255)
                                                                              cgccaCGGCAGCGGCGAACGTAAAAACGTTAGCCAGCGAGAAAAACGCGTTTTGATCATCACCAAAAATCCATAATAAATATAAGGTTATTGTAATAAAc                  <  2:1726602/100‑1 (MQ=255)
                                                                              cgccaCGGCAGCGGCGAACGTAAAAACGTTAGCCAGCGAGAAAAACGCGTTTTGATCATCACCAAAAATCCATAATAAATATAAGGTTATTGTAATAAAc                  <  2:1673791/100‑1 (MQ=255)
                                                                               gcaaCGGCAGCGGCGAACGTAAAAACGTTAGCTAGCGAGAAAAACGCGTTTTGATCATCACAAAAAATCCATAATAAATATAAGGTTATTGTAATAAACa                 <  2:1246646/97‑1 (MQ=255)
                                                                                 caCGGCAGCGGCGAACGTAAAAACGTTAGCCAGCGAGAAAAACGCGTTTTGATCATCACCAAAAATCCATAATAAATATAAGGTTATTGTAATAAACAGa               <  2:2341962/100‑1 (MQ=255)
                                                                                  aCGGCAGCGGCGAACGTAAAAACGTTAGCCAGCGAGAAAAACGCGTTTTGATCATCACCAAAAATCCATAATAAATATAAGGTTATTGTAATAAACAGa               <  1:618994/99‑1 (MQ=255)
                                                                                  aCGGCAGCGGCGAACGTAAAAACGTTAGCCAGCGAGAAAAACGCGTTTTGATCATCACCAAAAATCCATAATAAATATAAGGTTATTGTAATAAACAGAt              <  1:2423107/100‑1 (MQ=255)
                                                                                   cGGCAGCTGCGAACGTAAAAACGTTAGCCAGCGAGAAAAACGCGTTTTGATCATCACCAAAAATCCATAATAAATATAAGGTTATTGTAATAAACAGATa             <  1:556239/100‑1 (MQ=255)
                                                                                     gCAGCGGCGAACGTAAAAACGTTAGCCAGCGAGAAAAACGCGTTTTGATCATCACCAAAAATCCATAATAAATATAAGGTTATTGTAATAAACaagatcg           >  1:2145075/1‑94 (MQ=255)
                                                                                         cggcgAACGTAAAAACGTTAGCCAGCGAGAAAAACGCGTTTTGAACATAACCAAAAATCCATAATAAATATAAGGTTATTGGAATAAACAGATAAGCCTg       >  2:2462560/1‑100 (MQ=255)
                                                                                          ggcgAACGTAAAAAACGTTAGCCAGCGAGAAAAACGCGTTTTGATCATCACCAAAAATCCATAATAAATATAAGGTTATTGTAATAAACAGATAAGCCTg       >  1:2183922/1‑100 (MQ=255)
                                                                                          ggcgAACGTAAAAACGTTAGCCAGCGAGAAAAACGCGTTTTGATCATCACCAAAAATCCATAATAAATATAAGGTTATTGTAATAAACAGATAAGCCTGa      <  2:423881/100‑1 (MQ=255)
                                                                                            cgAACGTAAAAACGTTAGCAAGCGAGAAAAACGCGTTTTGATCATCACCAAAAATCCATAATAAATATAAGGTTATTGTAATAAACAGATAAGCCTGAAt    <  2:736496/100‑1 (MQ=255)
                                                                                             gAACGTAAAAACGTTAGCCAGCGAGAAAAACGCGTTTTGATCATCACCAAAAATCCATAATAAATATAAGGTTATTGTAATAAACAGATAAGCCTGAAtt   <  2:1687575/100‑1 (MQ=255)
                                                                                             gAACGTAAAAACGTTAGCCAGCGAGAAAAACGCGTTTTGATCATCACCAAAAATCCATAATAAATATAAGGTTATTGTAATAAACAGATAAGCCTGAAtt   >  1:1568346/1‑100 (MQ=255)
                                                                                              aaCGTAAAAACGTTAGCCAGCGAGAAAAACGCGTTTTGATCATCACCAAAAATCCATAATAAATATAATGTTATTGTAATAAACAGATAAGCCTGAATTa  <  2:2324134/100‑1 (MQ=255)
                                                                                                   |                                                                                               
ATGAAATTTGTTTTCGCCAACCAGACGACTGCCCGCTGGCGGCAGCGGCCAGGTGTTGGCTTTTGCCGGTAGCGCCAGCGCCACGGCAGCGGCGAACGTAAAAAACGTTAGCCAGCGAGAAAAACGCGTTTTGATCATCACCAAAAATCCATAATAAATATAAGGTTATTGTAATAAACAGATAAGCCTGAATTA  >  NC_000913/1170234‑1170428

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: