Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,446,362 Δ1 bp 17.7% intergenic (+156/+16) ydbL → / ← feaR DUF1318 family protein/transcriptional activator for tynA and feaB

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,446,3620A.17.7% 261.8 / 55.7 96intergenic (+156/+16)ydbL/feaRDUF1318 family protein/transcriptional activator for tynA and feaB
Reads supporting (aligned to +/- strand):  ref base A (38/41);  new base . (12/5);  total (50/46)
Fisher's exact test for biased strand distribution p-value = 1.13e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.76e-01

CTGAAACAAGGTGAATATTCAAAAACTCCTGTCAAATTGCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCAGTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCCCAAAGCGTTGCTTAAATACCGTTGAAAAATGACTCTGGTCAGAAA  >  NC_000913/1446263‑1446460
                                                                                                    |                                                                                                  
cTGAAACAAGGTGAATATTCAAAAACTCCTGTCAAATTGCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCAGTACa                                                                                                    >  2:2274850/1‑100 (MQ=255)
cTGAAACAAGGTGAATATTCAAAAACTCCTGTCAAATTGCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCAGTACa                                                                                                    >  2:2657986/1‑100 (MQ=255)
  gAAACAAGGTGAATATTCAAAAACTCCTGTCAAATTGCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCAGTACATg                                                                                                  <  1:2366278/100‑1 (MQ=255)
   aaaCAAGGTGAATATTCAAAAACTCCTGTCAAATTGCCTTTTGCTCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCAGTACATGc                                                                                                 <  1:195224/100‑1 (MQ=255)
        aGGTGAATATTCAAAAACTCCTGTCAAATTGCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCATGTACTGCAACGc                                                                                            <  1:370003/100‑1 (MQ=38)
         ggTGAATATTCAAAAACTCCTGTCAAATTGCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCATGTACTGCAACGcc                                                                                           <  1:2870668/100‑1 (MQ=38)
         ggTGAATATTCAAAAACTCCTGTCAAATTGCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCAGTACATGCAACGcc                                                                                           >  1:986798/1‑100 (MQ=255)
         ggTGAATATTCAAAAACTCCTGTCAAATTGCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCAGTACATGCAACGcc                                                                                           >  2:2533520/1‑100 (MQ=255)
         ggTGAATATTCAAAAACTCCTGTCAAATTGCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGAGTTGCAGTACATGCAACGc                                                                                            >  1:508835/1‑99 (MQ=255)
           tGAATATTCAAAAACTCCTGTCAAATTGCCTTTTTCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCAGTACATGCAACGcc                                                                                           <  1:2971739/98‑1 (MQ=255)
           tGAATATTCAAAAACTCCTGTCAAATTGCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCAGTACATGCAACGCCaa                                                                                         <  2:2771722/100‑1 (MQ=255)
            gAATATTCAAAAACTCCTGTCAAATTGCCTTTTGTGCTGAAAAATGCATAGTCAAATATCGGTTTTAACTAATTGGCGTTGCAGTACATGCAACGCCAAt                                                                                        <  1:1019089/100‑1 (MQ=255)
             aaaatacAAAAACTCCTGTCAAATTGCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCATGTACTGCAACGCCaa                                                                                         <  2:1616566/92‑1 (MQ=37)
             aaTATTCAAAAACTCCTGTCAAATTGCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCAGTACATGCAACGCCAAtt                                                                                       <  1:91037/100‑1 (MQ=255)
               tatTCAAAATCTCCTGTCAAATTGTCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCAGTACATGCAACGCCAAt                                                                                        <  1:2551335/97‑1 (MQ=255)
               tatTCAAAAACTCCTGTCAAATTACATAAAAAAAAAAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCAGTACATGCAACGCCAAttag                                                                                     <  2:1028722/100‑1 (MQ=25)
                    aaaaaCTCCTGTCAAATTGCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCAGTACATGCAACGCCAATTAGTTAGc                                                                                >  1:1525350/1‑100 (MQ=255)
                    aaaaaCTCCTGTCAAATTGCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCAGTACATGCAACGCCAATTAGTTAGc                                                                                >  2:225339/1‑100 (MQ=255)
                     aaaaCTCCTGTCAAATTGCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCAGTACATGCAACGCCAATTAGTTAGCg                                                                               <  2:3039404/100‑1 (MQ=255)
                         cTCCTGTCAAATTGCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCAGTACATGCAACGCCAATTAGTTAGCGGAAt                                                                           <  1:1762711/100‑1 (MQ=255)
                         cTCCTGTCAAATTGCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCAGTACATGCAACGCCAATTAGTTAGCGGAAt                                                                           <  1:1382777/100‑1 (MQ=255)
                           ccTGTCAAATTGCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCAGTACATGCAACGCCAATTAGTTAGCGGAAttt                                                                         <  2:2275565/100‑1 (MQ=255)
                             tGTCAGATTTCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCAGTACATGCTACGCTAATTAGTTAGCGGAATTTa                                                                        <  2:2018027/99‑1 (MQ=255)
                              gTCAAATTGCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCAGTACATGCAACGCCAATTAGTTAGCGGAATTTACg                                                                      >  1:1060354/1‑100 (MQ=255)
                              gTCAAATTGCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCAGTACATGCAACGCCAATTAGTTAGCGGAATTTACg                                                                      >  2:678298/1‑100 (MQ=255)
                               tCAAATTGCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCAGTACATGCAACGCCAATTAGTTAGCGGAATTTACGt                                                                     <  2:1339658/100‑1 (MQ=255)
                                 aaaTTGCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCAGTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCg                                                                   >  2:960147/1‑100 (MQ=255)
                                    ttGCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCAGTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATa                                                                >  1:2403014/1‑100 (MQ=255)
                                    ttGCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCAGTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATa                                                                >  1:2403255/1‑100 (MQ=255)
                                          tttGCCCTGAAATATGCATAGTCAACTATCTGTTTTAACTAATTGGCCTTGTAATACATGCAACATCAATTAGTTAGCTTAATTTACGTCGATAGTCGcc                                                          <  2:174552/100‑1 (MQ=255)
                                          tttGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCAGTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGcc                                                          >  1:1448441/1‑100 (MQ=255)
                                             gCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCAGTACATGCAATGCCAATGAGTTAGCGGAATTTACGTCGATACTCGCCTgg                                                       >  2:2133905/1‑100 (MQ=255)
                                              ctcTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCAGTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTgg                                                       <  1:655075/97‑1 (MQ=255)
                                              cccTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCAGTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGc                                                      >  1:2804623/1‑100 (MQ=255)
                                              cccTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCAGTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGc                                                      >  1:546199/1‑100 (MQ=255)
                                               ccTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCAGTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGc                                                      >  2:279596/1‑99 (MQ=255)
                                               ccTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCAGTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCg                                                     <  2:2403227/100‑1 (MQ=255)
                                               ccTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCAGTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCg                                                     <  2:1005663/100‑1 (MQ=255)
                                               ccTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCAGTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCg                                                     >  2:2255290/1‑100 (MQ=255)
                                               ccTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCAGTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCg                                                     <  1:1608710/100‑1 (MQ=255)
                                               ccTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCAGTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCg                                                     <  2:2402986/100‑1 (MQ=255)
                                                cTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCAGTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGt                                                    >  1:171294/1‑100 (MQ=255)
                                                 tGAAAAATGCTTATTCAAATTTTTGTTTTAACTATTTGGCGTTGCAGTACATGCAACGCCAATTAGTGAGCGGAATTTACGTCGATACTCGCCTGGCGt                                                    <  1:1974280/99‑1 (MQ=38)
                                                 tGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCAGTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTc                                                   >  2:1297026/1‑100 (MQ=255)
                                                 tGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCAGTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTc                                                   >  2:2008007/1‑100 (MQ=255)
                                                    aaaaTGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCAGTACATGCAACGCCAATTATTTAGCGGAAATTACGACGATACACGCCTGGCGTCa                                                  >  2:1440512/1‑98 (MQ=39)
                                                      aaTGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCAGTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATccc                                              <  1:1549084/100‑1 (MQ=255)
                                                      aaTGCAGAGTCAGATATCTGTTTTAACTAATTCGCGTTGCAGTACAGGCAACTCCAATTAGTTATCGGAATTTACGTCTATTCTCGCCTGGCGtcctcc                                               <  2:2641491/99‑1 (MQ=255)
                                                          cATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCAGTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCCCAAAg                                          >  2:1374596/1‑100 (MQ=255)
                                                          cATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCAGTACATGCAACGCCAATTAGTTAGCAAAATTTACGACAATACTCACCTGGCGTCATCCCAAAg                                          >  1:889475/1‑100 (MQ=38)
                                                           aTAGTCAAATATCTGTTTTAACTAATTGGCGTTGCAGTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCCCAAAGc                                         <  2:1211266/100‑1 (MQ=255)
                                                            tAGTCAAATATCTGTTTTAACTAATTGACGTTGCAGTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGCTACTCGCCTGGCGTCATCCCAAAGCg                                        >  2:659741/1‑100 (MQ=255)
                                                             aGTCAAATATCTGTTTTAACTAATTGGCGTTGCAGTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCCCAAAGCGt                                       <  1:795950/100‑1 (MQ=255)
                                                              gTCAAATATCTGTTTTAACTAATTGGCGTTGCAGTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCCCAAAGCGtt                                      >  1:2356389/1‑100 (MQ=255)
                                                              gTCAAATATCTGTTTTAACTAATTGGCGTTGCAGTACATGCAACGCCAATTAGTTAGAGGAATTTACGTAGAAAATCGCCTGGCGGCATCCAAAAGCGtt                                      >  1:2475091/1‑100 (MQ=38)
                                                              gTCAAATATCTGTTTGAACTAATTGGCGTTGCAGTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCCCAAAGCGtt                                      <  1:2993774/100‑1 (MQ=255)
                                                               tCAAATATCTGTTTTAACTAATTGGCGTTGCAGTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCCCAAAGCg                                        >  2:1937827/1‑97 (MQ=255)
                                                                cAAATATCTGTTTTAACTAATTGGCGTTGCAGTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCCCAAAGCGTTGc                                    <  1:127936/100‑1 (MQ=255)
                                                                cAAATATCTGTTTTAACTAATTGGCGTTGCAGTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCCCAAAGCGTTGc                                    <  1:788045/100‑1 (MQ=255)
                                                                 aaaTATCTGTTTTAACTAATTGGCGTTGCAGTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCCCAAAGCGTTGCt                                   <  1:2214267/100‑1 (MQ=255)
                                                                 aaaTATCTGTTTTAACTAATTGGCGTTGCAGTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCCCAAAGCGTTGCt                                   >  1:780681/1‑100 (MQ=255)
                                                                 aaaTATCTGTTTTAACTAATTGGCGTTGCAGTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCCCAAAGCGTTGCt                                   <  2:1593730/100‑1 (MQ=255)
                                                                  aaTATCTGTTTTAACTAATTGGCGTTGCAGTACATGCAACGCAAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCCCAAAGCGTTGc                                    <  1:1138863/98‑1 (MQ=255)
                                                                   atatCTGTTTTAACTAATTGGCGTTGCAGTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCCCAAAGCGTTGCTTa                                 <  1:1118876/100‑1 (MQ=255)
                                                                   atatCTGTTTTAACTAATTGGCGTTGCAGTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCCCAAAGCGTTGCTTa                                 <  1:166984/100‑1 (MQ=255)
                                                                         gTTTTAACTAATTGGCGTTGCAGTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCCCAAAGCGTTGCTTAAATAcc                           <  1:2188399/100‑1 (MQ=255)
                                                                           tttAACTAATTGGCGTTGCAGTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCCCAAAGCGTTGCTTAAATACCGt                         <  1:2529077/100‑1 (MQ=255)
                                                                              cgatctttGGCGTTGCATGTACTGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCCCAAAGCGTTGCTTAAATACCGTTGa                      <  1:1984547/94‑1 (MQ=37)
                                                                               aCTAATTGGCGTTGCATGTACTGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCCCAAAGCGTTGCTTAAATACCGTTGaa                     >  2:99090/1‑100 (MQ=37)
                                                                               aCTAATTGGCGTTGCAGTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCCCAAAGCGTTGCTTAAATACCGTTGaa                     >  1:372620/1‑100 (MQ=255)
                                                                                  aaTTGGCGTTGCATGTACTGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCCCAAAGCGTTGCTTAAATACCGTTGaaaaa                  >  1:1055325/1‑100 (MQ=38)
                                                                                  aaTTGGCGTTGCAGTACATGCAACGCCAATAAGATAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCCCAAAGCGTTGCTTAAATAGCGTTGaaaaa                  >  2:1997907/1‑100 (MQ=255)
                                                                                   aTTGGCGTTGCATGTACTGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCCCAAAGCGTTGCTTAAATACCGTTGAAAAAt                 >  1:541342/1‑100 (MQ=38)
                                                                                   aTTGGCGTTGCAGTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCCCAAAGCGTTGCTTAAATACCGTTGAAAAAt                 <  1:2617999/100‑1 (MQ=255)
                                                                                    ttGGCGTTGCATGTACTGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCCTCCCAAAGCGTTGCTTAAATACCGTTGAAAAATg                >  2:2892689/1‑100 (MQ=38)
                                                                                    ttGGCGTTGCATGTACTGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCCCAAAGCGTTGCTTAAATACCGTTGaagatcg                >  2:1984524/1‑95 (MQ=37)
                                                                                    ttGGCGTTGCATGTACTGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCCCAAAGCGTTGCTTAAATACCGTTGAAAAATg                >  2:2881036/1‑100 (MQ=38)
                                                                                    ctGGCGTTGCATGTACTGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCCCAAAGCGTTGCTTAAATACCGTTGAAAAATg                <  1:258831/99‑1 (MQ=38)
                                                                                     tGGCGTTGCATGTACTGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCCCAAAGCGTTGCTTAAATACCGTTGAAAAATGa               >  2:1684699/1‑100 (MQ=38)
                                                                                      ggCGTTGCATGTACTGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCCCAAAGCGTTGCTTAAATACCGTTGAAAAATGag              >  2:258828/1‑99 (MQ=38)
                                                                                      ggCGTTGCATGTACTGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGACGTCATCACAAAGAGTTGCTTAAATACCGTTGAAAAATGAc              >  1:190397/1‑100 (MQ=37)
                                                                                      ggCGTTGCATGTACTGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTAGCCTAGCGTCATCCCAAAAAGTTGCTTAAATACAGTTGAAAAATTAc              >  1:659733/1‑100 (MQ=25)
                                                                                      ggCGTTGCATGTACTGCAACGCCAATTAGATAGCGGAATTTACGTCAATACTCGCCTGACGTAATACCAAAGCGTTGCTTAAATACCGTTGAAAAATGa               >  2:1261770/1‑99 (MQ=25)
                                                                                      ggCGTTGCAGTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCCCAAAGCGTTGCTTAACTCCCGTTGAAAAATGAc              >  2:600868/1‑100 (MQ=255)
                                                                                      ggCGTTGCAGTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCCCAAAGCGTTGCTTAAATACCGTTGAAAAATGAc              >  1:2781564/1‑100 (MQ=255)
                                                                                       gCGTTGCATGTACTGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCCCAAAGCGTTGCTTAAATACCGTTGAAAAATGAct             >  2:1553514/1‑100 (MQ=38)
                                                                                        cGTTGCAGTACATGCAACGCAAATTAGTTAGCGGAATTTACGTCGATTCTCGCCTGGCGTCATACCAAAGCGTTGCTTAAATACCGTTGAAAAATGAc              >  2:3025855/1‑98 (MQ=255)
                                                                                         gTTGCAGTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCCCAAAGCGTTGCTTAAATACCGTTGAAAAATGActct           >  2:1544217/1‑100 (MQ=255)
                                                                                          ttGCAGTACATGCAACGACAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCCCAAAGCGTTGCTTAAATACCGTTGAAAAATGActc            <  1:2296762/98‑1 (MQ=255)
                                                                                            gCAGTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCCCAAAGCGTTGCTTAAATACCGTTGAAAAATGACTCTGGt        >  1:877703/1‑100 (MQ=255)
                                                                                            gCAGTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCCCAAAGCGTTGCTTAAATACCGTTGAAAAATGACTCTGGt        >  1:1499837/1‑100 (MQ=255)
                                                                                            gCAGTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCCCAAAGCGTTGCTTAAATACCGTTGAAAAATGACTCTGGt        <  1:1544235/100‑1 (MQ=255)
                                                                                             cAGTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCCCAAAGCGTTGCTTAAATACCGTTGAAAAATGACTCTGGTc       <  2:2458342/100‑1 (MQ=255)
                                                                                               aatACATGCAACGCTAAATAAATAGTAGAATTTACATAGATACTTGACTTGCGTCATCCCAAAGCGTTGTTTAAATAACTTTAAAAAATGACTCTGGTc       <  2:951121/97‑1 (MQ=255)
                                                                                                gTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCCCAAAGCGTTGCTTAAATACCGTTGAAAAATGACTCTGGTCAg     >  1:1774701/1‑100 (MQ=255)
                                                                                                gTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCCCAAAGCGTTGCTTAAATACCGTTGAAAAATGACTCTGGTCAg     <  1:469102/100‑1 (MQ=255)
                                                                                                  aCATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCCCAAAGCGTTGCTTAAATACCGTTGAAAAATGACTCTGGTCAGaa   >  1:2379855/1‑100 (MQ=255)
                                                                                                   cATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCCCAAAGCGTTGCTTAAATACCGTTGAAAAATGACTCTGGTCAGaaa  >  1:180402/1‑100 (MQ=255)
                                                                                                    aTGCAACGCCAATTAGTTAGCGGAATTTACGTCGTTACTCGCCTGGCTTCATCCCAAAGCGTTGCTTAAATACCGTTGAAAAATGACTCTGGTCAGaa   <  2:2966135/98‑1 (MQ=255)
                                                                                                    |                                                                                                  
CTGAAACAAGGTGAATATTCAAAAACTCCTGTCAAATTGCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCAGTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCCCAAAGCGTTGCTTAAATACCGTTGAAAAATGACTCTGGTCAGAAA  >  NC_000913/1446263‑1446460

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: