Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,520,504:1 +T 16.9% intergenic (‑180/+167) yjhU ← / ← yjhF putative DNA‑binding transcriptional regulator; KpLE2 phage‑like element/putative transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,520,5041.T16.9% 395.8 / 87.0 124intergenic (‑180/+167)yjhU/yjhFputative DNA‑binding transcriptional regulator; KpLE2 phage‑like element/putative transporter
Reads supporting (aligned to +/- strand):  ref base . (60/43);  new base T (8/13);  total (68/56)
Fisher's exact test for biased strand distribution p-value = 9.94e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.22e-01

Only 100 of 126 total aligned reads displayed.

ATGATTTTATACAGATATTTTATTCTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATGCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATCGCCAGCGATTCGTCACCGTGCAGA  >  NC_000913/4520405‑4520602
                                                                                                    |                                                                                                  
aTGATTTTATACAGATATTTTATTCTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATg                                                                                                     >  2:1918563/1‑100 (MQ=255)
   aTTTTATACAGATATTTTATTCTTTTACATGAAATAAACCAGCATACAATTTGAGCGATAGCAAGCTGTTACCCCATACTGTAAAGCCGGAGACATg                                                                                                     >  2:2447217/1‑97 (MQ=255)
      ttATACAGATATTTTATTCTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATGCTCCgg                                                                                              <  2:2911167/100‑1 (MQ=255)
       tataCAGATATTTTATTCTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATGCTCCGGc                                                                                             >  1:3026007/1‑100 (MQ=255)
       tataCAGATATTTTATTCTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATGCTCCGGc                                                                                             <  2:1856768/100‑1 (MQ=255)
       tataCAGATATTTTATTCTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGCATGTCTCCGGc                                                                                             <  2:794034/100‑1 (MQ=39)
         taCAGATATTTTATTCTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATGCTCCGGCtt                                                                                           <  2:104223/100‑1 (MQ=255)
         taCAGATATTTTATTCTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATGCTCCGGCtt                                                                                           <  2:795995/100‑1 (MQ=255)
         taCAGATATTTTATTCTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGCATGTCTCCGGCtt                                                                                           <  1:2215314/100‑1 (MQ=38)
         taCAGATATTTTATTCTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGCATGTCTCCGGCtt                                                                                           <  2:796265/100‑1 (MQ=38)
          aCATATATTTTATTCTTTTACATGAAATAAACCATCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGCATGTCTCCGGCttt                                                                                          <  2:929328/100‑1 (MQ=37)
          aCAGATATTTTATTCTTTTACATGATATAAACCAGCATACCATTTGATCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGCATGTCTCCGGCtt                                                                                           <  2:1695867/99‑1 (MQ=37)
          aCAGATATTTTATTCTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATGCTCCGGCttt                                                                                          <  1:1704532/100‑1 (MQ=255)
          aCAGATATTTTATTCTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGCATGTCTCCGGCttt                                                                                          <  2:325829/100‑1 (MQ=38)
             tcttccgatctCTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGCATGTCTCCGGCTTTACa                                                                                       <  1:1950119/90‑1 (MQ=25)
             gATATTTTATTCTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATGCTCCGGCTTTACa                                                                                       <  1:2717882/100‑1 (MQ=255)
             gATATTTTATTCTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGCATGTCTCCGGCTTTACa                                                                                       <  1:1737504/100‑1 (MQ=38)
             gATATTTTATTCTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGCATGTCTCCGGCTTTACa                                                                                       <  1:567151/100‑1 (MQ=38)
              atatTTTATTCTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGCATGTCTCCGGCTTTACAg                                                                                      <  1:910657/100‑1 (MQ=38)
              atatTTTATTCTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGCATGTCTCCGGCTTTACAg                                                                                      <  1:1457707/100‑1 (MQ=38)
               tatTTTATTCTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACAAACTCCGGCTTTACAGt                                                                                     >  1:2924010/1‑100 (MQ=255)
                atTTTATTCTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATGCTCCGGCTTTACAGTa                                                                                    >  1:908986/1‑100 (MQ=255)
                actttATTCTTTTACATGAAATAAACCAGCATACCATTTGGTCGATCGCCCGCTTTTCCCCCATACTGTAAAGCCTGATACATGCTCCGGCTTTACAGt                                                                                     <  2:1481318/97‑1 (MQ=255)
                 ttttATTCTTTTACATGAAATAAACCAGCATGCCATTTGAGCGATCGCCCGCTTTTCCCCCATACTGTAAAGTCGTAGACATGCTCCGGCTTTACAGTa                                                                                    <  2:2682777/99‑1 (MQ=255)
                      ttCTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATGCTCCGGCTTTACAGTATTACt                                                                               >  2:2184237/1‑99 (MQ=255)
                      ttCTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAACCGGAGACATGCTCCGGCTTTACAGTATTACTg                                                                              >  1:2600720/1‑100 (MQ=255)
                       tCTTTTACATGAAATCAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATGCTCCGGCTTTACAGTATTACTga                                                                             <  1:3000453/100‑1 (MQ=255)
                        cTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATGCTCCGGCTTTACAGTATTACTgag                                                                            >  1:122035/1‑100 (MQ=255)
                        cTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATGCTCCGGCTTTACAGTATTACTgag                                                                            >  2:472127/1‑100 (MQ=255)
                        cTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGCATGTCTCCGGCTTTACAGTAtgggggaa                                                                            <  2:2930499/100‑8 (MQ=21)
                         ttttACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATGCTCCGGCTTTACAGTATTACTgaga                                                                           <  1:2840008/100‑1 (MQ=255)
                         ttttACATGAAATAAACCAGAATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATGCTCCGGCTTTACAGTATTACTgaga                                                                           >  2:161558/1‑100 (MQ=255)
                          tttACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAAACATGCTCCGGCTTTACAGTATTACTGAGaa                                                                          >  1:502247/1‑100 (MQ=255)
                            taactGAACTAAACCAGCATACCATTTGTGCGATCGCCCGCTGTTGCCCCATACTGTAAAGCGTTAGACATGCTCCGGTTTTACAGTATGACTgag                                                                            <  2:2904867/92‑1 (MQ=255)
                              cATGAAATAAACCAGTATACCATTTGAGCGATCGCTCACTGTTCCCCCATACTGTAAATCCTGAGACATGCTCCGGCTTTACAGTATTACTGAGAATaca                                                                      >  2:2660536/1‑100 (MQ=255)
                              cATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATGCTCCGGCTTTACGGTATTACTGAGAATaca                                                                      >  1:2170524/1‑100 (MQ=255)
                              cATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATGCTCCGGCTTTACAGTATTACTGAGAATaca                                                                      >  2:1474810/1‑100 (MQ=255)
                              cATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATGCTCCGGCTTTACAGTATTACTGAGAATaca                                                                      >  2:959972/1‑100 (MQ=255)
                              cATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCACCATACTGTAAAGCCGGAGACCTGCTACGGCTTTACAGTATTATTGAGAATcca                                                                      >  2:245667/1‑100 (MQ=255)
                                tGAAATAAACCAGCATACCATTTGTGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATGCTCCGGCTTTACAGTATTACTGAGAATACAcc                                                                    <  1:1290244/100‑1 (MQ=255)
                                tGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAATCCGGAGACATGCTCCAGCTTTACAGTATTACTGAGAATACAcc                                                                    <  2:1136347/100‑1 (MQ=255)
                                tGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATGCTCCGGCTTTACAGTATTACTGAGAATacac                                                                     <  2:407344/99‑1 (MQ=255)
                                tGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATGCGCCGGCTTTACAGTATTACTGAGAATACAcc                                                                    <  2:1996617/100‑1 (MQ=255)
                                  aaaTAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATGCTCCGGCTTTACAGTATTACTGAGAATACACCCa                                                                  >  1:2225646/1‑100 (MQ=255)
                                  aaaTAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATGCTCCGGCTTTACAGTATTACTGAGAATACACCCa                                                                  >  2:2254686/1‑100 (MQ=255)
                                  aaaTAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATGCTCCGGCTTTACAATATTACTGAGAATACACCCa                                                                  >  1:632569/1‑100 (MQ=255)
                                   aaTACACCAGCATACCATTTGAGCGATCGCCCGCTGTTCACCCATACTGTAAAGCAGGAGACATACTCCGCCTTTACAGTATTACAGAAAAAACACCCAt                                                                 >  2:2154147/1‑100 (MQ=255)
                                       aaCCAGCATACCATTTGAGCGATCGCCCGGTGTTCCCCCATACTGTAAAGCCGGAGACATGCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAg                                                             <  1:2219203/100‑1 (MQ=255)
                                       aaCCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCCTACTGTAAAGCCGGAGACATGCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAg                                                             >  1:3033402/1‑100 (MQ=255)
                                       aaCCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATGCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAg                                                             >  1:2331508/1‑100 (MQ=255)
                                        aCCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCAGACTGTAAAGCCGGAGACATGCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGc                                                            <  2:2331481/100‑1 (MQ=255)
                                              aTACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATGCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATc                                                      >  1:1496197/1‑100 (MQ=255)
                                               tACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATGCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCg                                                     >  1:1082136/1‑100 (MQ=255)
                                               tACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATGCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCg                                                     <  1:2195176/100‑1 (MQ=255)
                                               tACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATGCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCg                                                     >  2:1176147/1‑100 (MQ=255)
                                                aCCATTTGGGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATGCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCg                                                     <  2:2225623/99‑1 (MQ=255)
                                                aCCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATGCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGa                                                    >  2:673321/1‑100 (MQ=255)
                                                 ccATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATGCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGaa                                                   >  1:1671159/1‑100 (MQ=255)
                                                 ccATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATGCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGaa                                                   >  1:2789301/1‑100 (MQ=255)
                                                   aTTTGAGCGATCGCCCGCTGTTCCTCCATACTGTAAAGCCGGAGACATGCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAgg                                                 <  2:533105/100‑1 (MQ=255)
                                                    tttGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATGCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGaa                                                   >  2:1306386/1‑97 (MQ=255)
                                                      tGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATGCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTc                                              <  1:2706918/100‑1 (MQ=255)
                                                       gAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATGCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCa                                             >  1:2385567/1‑100 (MQ=255)
                                                        aGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATGCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCaa                                            <  2:230406/100‑1 (MQ=255)
                                                         gCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATGCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAat                                           >  1:2221446/1‑100 (MQ=255)
                                                              cGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATGCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGc                                      <  2:2113555/100‑1 (MQ=255)
                                                               gCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATGCTCCGGCTTTACAGTATTACTGAGAGTACACTCATCAAGCATAATCGAAGGTTCAATATCGc                                      <  2:2114975/99‑1 (MQ=255)
                                                               gCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATGCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGcc                                     >  2:943851/1‑100 (MQ=255)
                                                               gCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATGCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGcc                                     >  2:2905147/1‑100 (MQ=255)
                                                                cccGCTGTTCCCCCATACTGTAAAGCCGGAGACATTCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGccc                                    <  1:902725/100‑1 (MQ=255)
                                                                cccGCTGTTCCCCCATACTGTAAAGCCGGAGACATGCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGccc                                    <  2:2614236/100‑1 (MQ=255)
                                                                     ttctcagtaaTACTGTAAAGCCGGAGCATGTCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCtttt                               <  1:1573612/91‑1 (MQ=21)
                                                                       ttCCCCCATACTGTAAAGCCGGAGACATGCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTg                             <  2:2700587/100‑1 (MQ=255)
                                                                           cccATACTGTAAAGCCGGAGACATGCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATCg                         >  1:3019787/1‑100 (MQ=255)
                                                                           cccATACTGTAAAGCCGGAGACATGCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATCg                         <  1:2689461/100‑1 (MQ=255)
                                                                           cccATACTGTAAAGCCGGAGACATGCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATCg                         >  2:1705731/1‑100 (MQ=255)
                                                                            ccATACTGTAAAGCCGGAGACATGCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGACCCTTTTTGATCGc                        >  2:2067240/1‑100 (MQ=255)
                                                                             cATACTGTAAAGCCGGAGACATGCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATCGcc                       >  1:81511/1‑100 (MQ=255)
                                                                             cATACTGTAAAGCCGGAGACATGCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATCGcc                       <  2:2265585/100‑1 (MQ=255)
                                                                             cATACTGTAAAGCCGGAGACATGCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATCGcc                       >  1:2907344/1‑100 (MQ=255)
                                                                               tACTGTAAAGCCGGAGACATGCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATCGCCAg                     >  1:2169233/1‑100 (MQ=255)
                                                                                aCTGTAAAGCCGGAGCGTGTCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGAATAATCGAAGGTTCAATATCGCCCCTTTTTGATCGCCAg                     >  1:586548/1‑99 (MQ=37)
                                                                                 cTGTAAAGCCGGAGACATGCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATCGCCAGCg                   >  2:768364/1‑100 (MQ=255)
                                                                                 cTGTAAAGCCGGAGCATGTCTCCGGCTTTACAGTATTACTGAGAATACACACATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATCGCCAGCg                   >  1:23834/1‑100 (MQ=37)
                                                                                  tGTAAAGCCGGAGACATGCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATCGCCAGCGa                  >  2:170106/1‑100 (MQ=255)
                                                                                   gTAAAGCCGGAGCATGTCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATCACCAGCGa                  >  1:1197632/1‑99 (MQ=37)
                                                                                    tAAAGCCGGAGCATGTCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATCGCCAGCGAtt                >  2:1474302/1‑100 (MQ=38)
                                                                                     aaaGCCGGAGACATGCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATCGCCAGCGATTc               >  2:3043967/1‑100 (MQ=255)
                                                                                     aaaGCCGGAGACATGCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGATTCAATATCGCCCCTTTTTGATCGCCAGCGATTc               >  1:2397734/1‑100 (MQ=255)
                                                                                     aaaGCCGGAGACATGCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCAAAGGTTCAATATCGCAACTTTTTGATCGCCATCGAtt                >  1:1362652/1‑99 (MQ=255)
                                                                                     aaaGCCGGAGCATGTCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATCGCCAGCGATTc               >  2:2977919/1‑100 (MQ=38)
                                                                                        gCCGGAGACATGCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCATTTTTGATCGCCAGCGATTCGTc            >  1:64006/1‑100 (MQ=255)
                                                                                        gCCGGAGCATGTCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATCGCCAGCGATTCGTc            >  1:2568817/1‑100 (MQ=39)
                                                                                          cGGAGACATGCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATAGCCCCTTTTTGATCGCCAGCGATTCGTCAc          >  2:1165096/1‑100 (MQ=255)
                                                                                             agaCATGCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATAGCCAGCGATTCGTCACCg        >  1:1481021/1‑99 (MQ=255)
                                                                                               aCATGCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATCGCCAGCGATTCGTCACCGTGc     <  1:803074/100‑1 (MQ=255)
                                                                                                cATGCTCCGTCTTTACAGTATTACTGAGAATACACTCATTAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATCGCCAGCGATTCGTCACCGTGCa    <  1:287950/100‑1 (MQ=255)
                                                                                                cATGCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATCGCCAGCGATTCGTCACCGTGCa    >  1:492485/1‑100 (MQ=255)
                                                                                                  tGCTCCGGCTTTACAGTATTACTGATAATACTCCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATCGCCAGCGATTCGTCACCGTGCAg   <  1:2533447/99‑1 (MQ=255)
                                                                                                  tGCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTTAATATCGCCCCTTTTTGATCCCCAGCGATTCGTCACCGTGCAGa  >  2:3032629/1‑100 (MQ=255)
                                                                                                    |                                                                                                  
ATGATTTTATACAGATATTTTATTCTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATGCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATCGCCAGCGATTCGTCACCGTGCAGA  >  NC_000913/4520405‑4520602

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: