Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,827,031 T→A 100% D410V (GAT→GTT)  astB ← succinylarginine dihydrolase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,827,0310TA98.0% 317.0 / ‑1.7 101D410V (GAT→GTT) astBsuccinylarginine dihydrolase
Reads supporting (aligned to +/- strand):  ref base T (2/0);  new base A (55/43);  total (58/43)
Fisher's exact test for biased strand distribution p-value = 5.05e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.20e-01

Only 100 of 101 total aligned reads displayed.

TCGCGCTGGAACGGATAAACCGAACCGAGATTCAGTAATTGGCTCAATACATCCAGTGCTTCCCGCCCTTCGCGCAGCAATTGCGGGTCGGCCAGATCGGCAGCAGTAAGGCGATCGCGGTAGTAACGATCCACCCAGTCATTGAGCGCATTAAACAGCGTATCGTTCATCATCACCGCCGGATTCACCGCCC  >  NC_000913/1826935‑1827127
                                                                                                |                                                                                                
tcGCGCTGGAACGGATAAACCGAACCGAGATTCAGTAATTGGCTCAATACATCCAGTGCTTCCCGCCCTTCGCGCAGCAATTGCGGGTCGGCCAGAACgg                                                                                               >  2:309640/1‑100 (MQ=255)
 cgcgCTGGAACGGATAAACCGAACCGAGATTCAGTAATTGGCTCAATACATCCAGTGCTTCCCGCCCTTCGCGCAGCAATTGCGGGTCGGCCAGAACg                                                                                                >  2:1302776/1‑98 (MQ=255)
 cgcgCTGGAACGGATAAACCGAACCGAGATTCAGTAATTGGCTCAATAAATCCAGTGCTTCCCGCCCTTAGCGCAGCAATTGCGGGTCGGCCAGAACg                                                                                                >  2:2700133/1‑98 (MQ=255)
 cgcgCTGGAACGGATAAACCGAACCGAGACTCAGTAATAGGCGCAATACATCCAGTGCTTCCCACCCTTCGCGCAGCAATTGCGGGTCGGCCAGACCg                                                                                                >  2:1875215/1‑98 (MQ=255)
   ctcttgAACGGATAAACCGAACCGGGATTCAGTAATTGGCTCAATACATCCAGTGCTTCCCGCCCTTCGCGCAGCAATTGCGGGTCGGCCAGAACGgca                                                                                             <  1:192352/94‑1 (MQ=255)
   cgcTGGAACGGATAAACCGAACCGAGATTCAGTAATTGGCTCAATACATCCAGTGCTTCCCGCCCTTCGCGCAGCAATTGCGGGTCGGCCAGAACGgcag                                                                                            >  1:811373/1‑100 (MQ=255)
    gcTGGAACGGATAAACCGAACCGAGATTCAGTAATTGGCTCAATACATCCAGTGCTTCCCGCCCTTCGCGCAGCAATTGCGGGTCGGCCAGAACGgcagc                                                                                           >  1:2913181/1‑100 (MQ=255)
    gcTGGAACGGATAAACCGAACCGAGATTCAGTAATTGGCTCAATACATCCAGTGCTTCCCGCCCTTCGCGCAGCAATTGCGGGTCGGCCAGAACGgcagc                                                                                           <  1:1820468/100‑1 (MQ=255)
    gcTGGAACGGATAAACCGAACCGAGATTCAGTAATTGGCTCAATACATCCAGTGCTGCCCGCCCTTCGCGCAGCAATTGCGGGTCGGCCAGAACGgcagc                                                                                           <  2:2775851/100‑1 (MQ=255)
     cTGGAACGGATAAACCGAACCGAGATTCAGTAATTGGCTCAATACATCCAGTGCTTCCCGCCCTTCGCGCAGCAATTGCGGGTCGGCCAGAACGgcagca                                                                                          >  1:2280/1‑100 (MQ=255)
       ggAACGGATAAACCGAACCGAGATTCAGTAATTGGCTCAATACATCCAGTGCTTCCCGCCCTTCGCGCAGCAATTGCGGGTCGGCCAGAACGGCAGCAGt                                                                                        >  1:2181617/1‑100 (MQ=255)
       ggAACGGATAAACCGAACCGAGATTCAGTAATTGGCTCAATACATCCAGTGCTTCCCGCCCTTCGCGCAGCAATTGCGGGTCGGCCAGAACGGCAGCAGt                                                                                        <  1:1654005/100‑1 (MQ=255)
       ggAACGGATAAACCGAACCGAGATTCAGTAATTGGCTCAATACATCCAGTGCTTCCCGCCCTTCGCGCAGCAATTGCGGGTCGGCCAGAACGGCAGCAGt                                                                                        >  1:1142398/1‑100 (MQ=255)
       ggAACGGATAAACCGAACCGAGATTCAGTAATTGGCTCAATACATCCAGTGCTTCCCGCCCTTCGCGCAGCAATTGCGGGTCGGCCAGAACGGCAGCAGt                                                                                        >  1:1181221/1‑100 (MQ=255)
        gAACGGATAAACCGAACCGAGATTCAGTAATTGGCTCAATACATCCAGTGCTTCCCGCCCTTCGCGCAGCAATTGCGGGTCGGCCAGAACGGCAGCAGTa                                                                                       <  1:1645012/100‑1 (MQ=255)
        gAACGGATAAACCGAACCGAGATTCAGTAATTGGCTCAATACATCCAGTGCTTCCCGCCCTTCGCGCAGCAAGTGCGAGTGGGCCAGAACagcagcccta                                                                                       >  2:2016147/1‑96 (MQ=255)
           cGGATAAACCGAACCGAGATTCAGTAATTGGCTCAATACATCCAGTGCTTCCCGCCCTTCGCGCAAAAATTGCGAGTCGGCCAGAACGgcagca                                                                                          >  1:2139574/1‑94 (MQ=255)
            ggATAAACCGAACCGAGATTCAGTAATTGGCTCAATACATCCAGTGCTTCCCGCCCTTCGCGCAGCAATTGCGGGTCGGCCAGAACGGCAGCAGTAAGGc                                                                                   >  1:2416156/1‑100 (MQ=255)
               tAAACCGAACCGAGATTCAGTAATTGGCTCAATACATCCAGTGCTTCCCGCCCTTCGCGCAGCAATTGCGGGTCGGCCAGAACGGCAGCAGTAAGGCGAt                                                                                >  2:8662/1‑100 (MQ=255)
                 aaccgaaccgAGATTCAGTAATTGGCTCAATACATCCAGTGCTTCCCGCCCTTCGCGCAGCAATTGAGGGTCGGCCATAACGGCAGCAGTAATGCGa                                                                                 >  2:2734368/1‑97 (MQ=255)
                  accgaaccgaGATTCAGTAATTGGCTCAATACATCCAGTGCTTCCCGCCCTTCGCGCAGCAATTGCGGGTCGGCCAGAACGGCAGCAGTAAGGCGATcgc                                                                             <  1:748956/100‑1 (MQ=255)
                   ccgaaccgaGATTCAGTAATTGGCTCAATACATCCAGTGCTTCCCGCCCTTCGCGCAGCAATTGCGGGTCGGCCAGAACGGCAGCAGTAAGGCGATcgcg                                                                            >  1:84476/1‑100 (MQ=255)
                     gaaccgaGATTCAGTAATTGGCTCAATACATCCAGTGCTTCCCGCCCTTCGCGCAGCAATTGCGGGTCGGCCAGAACGGCAGCAGTAAGGCGATCGCGgt                                                                          >  1:1802140/1‑100 (MQ=255)
                     gaaccgaGATTCAGTAATTGGCTCAATACATCCAGTGCTTCCCGCCCTTCGCGCAGCAATTGCGGGTCGGCCAGAACGGCAGCAGTAAGGCGATCGCGgt                                                                          >  1:1718744/1‑100 (MQ=255)
                        ccgaGATTCAGTAATTGGCTCAATACATCCAGTGCTTCCCGCCCTTCGCGCAGCAATTGCGGGTCGGCCAGAACGGCAGCAGTAAGGCGATCGCGgtagt                                                                       >  1:2206032/1‑100 (MQ=255)
                         cgaGATTCAGTAATTGGCTCAATACATCCAGTGCTTCCCGCCCTTCGCGCAGCAATTGCGGGTCGGCCAGAACGGCAGCAGTAAGGCGATCGCGgtagta                                                                      <  2:2398336/100‑1 (MQ=255)
                         cgaGATTCAGTAATTGGCTCAATACATCCAGTGCTTCCCGCCCTTCGCGCAGCAATTGCGGGTCGGCCAGAACGGCAGCAGTAAGGCGATCGCGggagta                                                                      >  2:1864615/1‑100 (MQ=255)
                           agaTTCAGTAATTGGCTCAATACATCCAGTGCTTCCCGCCCTTCGCGCAGCAATTGCGGGTCGGCCAGAACGGCAGCAGTAAGGCGATCGCGGTAGTAAc                                                                    <  1:376896/100‑1 (MQ=255)
                              ttCAGTAATTGGCTCAATACATCCAGTGCTTCCCGCCCTTCGCGCAGCAATTGCGGGTCGGCCAGAACGGCAGCAGTAAGGCGATCGCGGTAGTAACGAt                                                                 <  1:1202780/100‑1 (MQ=255)
                              ttCAGTAATTGGCTCAATACATCCAGTGCTTCCCGCCCTTCGCGCAGCAATTGCGGGTCGGCCAGAACGGCAGCAGTAAGGCGATCGCGGTAGTAACGAt                                                                 <  1:2258509/100‑1 (MQ=255)
                               tCAGTAATTGGCTCAATACATCCAGTGCTTCCCGCCCTTCTCGCAGCAATTGCGGGTCGGCCAGAACGGCAGCAGTAAGGCGATCGCGGTAGTAACGATc                                                                <  1:1065572/100‑1 (MQ=255)
                                cAGTAATTGGCTCAATACATCCAGTGCTTCCCGCCCTTCGCGCAGCAATTGCGGGTCGGCCAGAACGGCAGCAGTAAGGCGATCGCGGTAGTAACGATcc                                                               >  2:2148659/1‑100 (MQ=255)
                                cAGTAATTGGCTCAATACATACAGTGCTTCCCGCCCTTCGCGCAGCAATTGCGGGTCGGCCAGAACGGCAGCAGTAAGGCGATCGCGGTAGTAACGATc                                                                >  2:1135194/1‑99 (MQ=255)
                                    aaTTGGCTCAATACATCCAGTGCTTCCCGCCCTTCGCGCAGCAATTGCGGGTCGGCCAGAACGGCAGCAGTAAGGCGATCGCGGTAGTAACGATCCAccc                                                           <  1:889017/100‑1 (MQ=255)
                                    aaTTGGCTCAATACATCCAGTGCTTCCCGCCCTTCGCGCAGCAATTGCGGGTCGGCCAGAACGGCAGCAGTAAGGCGATCGCGGTAGTAACGATCCAccc                                                           <  1:975737/100‑1 (MQ=255)
                                      ttGGCTCAATACATCCAGTGCTTCCCGCCCTTCGCGCAGCAATTGCGGGTCGGCCAGAACGGCAGCAGTAAGGCGATCGCGGTAGTAACGATCCACCCac                                                         <  2:1370624/100‑2 (MQ=255)
                                      ttGGCTCAATACATCCAGTGCTTCCCGCCCTTCGCGCAGCAATTGCGGGTCGGCCAGAACGGCAGCAGTAAGGCGATCGCGGTAGTAACGATCCACCCAg                                                         <  2:2846805/100‑1 (MQ=255)
                                        ggCTCAATACATCCAGTGCTTCCCGCCCTTCGCGCAGCAATTGCGGGTCGGCCAGAACGGCAGCAGTAAGGCGATCGCGGTAGTAACGATCCACCCAGTc                                                       >  2:1123454/1‑100 (MQ=255)
                                        ggCTCAATACATCCAGTGCTTCCCGCCCTTCGCGCAGCAATTGCGGCTCGGCCAGAACGGCAGCAGTAAAGCGATCGCGGTAGTAACGATCCACCCAGTc                                                       >  1:2658029/1‑100 (MQ=255)
                                            cAATACATCCAGTGCTTCCCGCCCTTCGCGCAGCAATTGCGGGTCGGCCAGAACGGCAGCAGTAAGGCGATCGCGGTAGTAACGATCCACCCAGTCATTg                                                   <  2:2260192/100‑1 (MQ=255)
                                            cAATACATCCAGTGCTTCCCGCCCTTCGCGCAGCAATTGCGGGTCGGCCAGAACGGCAGCAGTAAGGCGATCGCGGTAGTAACGATCCACCCAGTCATTg                                                   <  1:1012050/100‑1 (MQ=255)
                                            cAATACATCCAGTGCTTCCCGCCCTTAGCGCAACAATTGCGGGACGGCCAGAACGGCAGCAGTAAGGCGATCGAGGTAGTAACGATCCACCCAGTCATTg                                                   >  2:335577/1‑100 (MQ=255)
                                             aaTACATCCAGTGCTTCCCGCCCTTCGCGCAGCAATTGCGGGTCGGCCAGAACGGCAGCAGTAAGGCGATCGCGGTAGTAACGATCCACCCAGTCATTGa                                                  >  1:3007320/1‑100 (MQ=255)
                                             aaTACATCCAGTGCTTCCCGCCCTTCGCGCAGCAATTGCGGGTCGGCCAGAACGGCAGCAGTAAGGCGATCGCGGTAGTAACGATCCACACAGTCAtt                                                    >  1:898092/1‑98 (MQ=255)
                                             aaTACATCCAGTGCTTCCCGCCCTTCGCGCAGCAATTGCGGGTCGGCCAGAACGGCAGCAGTAAGACGATCGCGGTAGTAACGATCCACCCAGTCATTGa                                                  >  2:2939143/1‑100 (MQ=255)
                                              aTACATCCAGTGCTTCCCGCCCTTCGCGCAGCAATTGCGGGTCGGCCAGATCGGCAGCAGTAAGGCGATCGCGGTAGTAACGATCCACCCAGTCATTGAg                                                 >  1:1395777/1‑100 (MQ=255)
                                              aTACATCCAGTGCTTCCCGCCCTTCGCGCAGCAATTGCGGGTCGGCCAGAACGGCAGCAGTAAGGCGATCGCGGTAGTAACGATCCACCCAGTCATTGAg                                                 >  1:1736703/1‑100 (MQ=255)
                                                aCATCCAGTGCTTCCCGCCCTTCGCGCAGCAATTGCGGGTCGGCCAGAACGGCAGCAGTAAGGCGATCGCGGTAGTAACGATCCACCCAGTCATTGAgcg                                               <  2:1256297/100‑1 (MQ=255)
                                                 cATCCAGTGCTTCCCGCCCTTCGCGCAGCAATTGCGGGTCGGCCAGAACGGCAGCAGTAAGGCGATCGCGGTAGTAACGATCCACCCAGTCATTGAgcgc                                              >  1:2617481/1‑100 (MQ=255)
                                                 cATCCAGTGCTTCCCGCCCTTCGCGCAGCAATTGCGGGTCGGCCAGAACGGCAGCAGTAAGGCGATCGCGGTAGTAACGATCCACCCAGGCATTGAg                                                 >  2:2201355/1‑97 (MQ=255)
                                                  aTCCAGTGCTTCCCGCCCTTCGCGCAGCAATTGCGGGTCGGCCAGAACGGCAGCAGTAAGGCGATCGCGGTAGTAACGATCCACCCAGTCATTGAGCGCa                                             >  1:2767914/1‑100 (MQ=255)
                                                  aTCCAGTGCTTCCCGCCCTTCGCGCAGCAATTGCGGGTCGGCCAGAACGGCAGCAGTAAGGCGATCGCGGTAGTAACGATCCACCCAGTCATTGAGCGCa                                             <  2:1802119/100‑1 (MQ=255)
                                                  aTCCAGTGCTTCCCGCCCTTCGCGCAGCAATTGCGGGTCGGCCAGAACGGCAGCAGTAAGGCGATCGAGGTAGTAAAGATCCACCCAGTCATTGAGCGCa                                             >  1:1958822/1‑100 (MQ=255)
                                                  aTCCAGTGCTTCCCGCCCTTCGCGCAGCAATTGCGGGTCGGCCAGAACGGCAGCAGCAAGGCGATCGCGGTAGTAACGATCCACCCAGTCATTGAGCGCa                                             >  2:1940325/1‑100 (MQ=255)
                                                    ccAGTGCTTCCCGCCCTTCGCGCAGCAATTGCGGGTCGGCCAGAACGGCAGCAGTAAGGCGATCGCGGTAGTAACGATCCACCCAGTCATTGAGCGCAtt                                           <  1:2099368/100‑1 (MQ=255)
                                                     cAGTGCTTCCCGCCCTTCGCGCAGCAATTGCGGGTCGGCCAGAACGGCAGCAGTAAGGCGATCGCGGTAGTAACGATCCACCCAGTCATTGAGCGCATTa                                          <  2:218297/100‑1 (MQ=255)
                                                     cAGTGCTTCCCGCCCTTCGCGCAGCAATTGCGGGTCGGCCAGAACGGCAGCAGTAAGGCGATCGCGGTAGTAACGATCCACCCAGTCATTGAGCGCATTa                                          >  1:1431910/1‑100 (MQ=255)
                                                     cAGTGCTTCCCGCCCTTCGCGCAGCAATTGCGGGTCGGCCAGAACGGCAGCAGTAAGGCGATCGCGGTAGTAACGATCCACCCAGTCATTGAGCGCATTa                                          >  1:1383661/1‑100 (MQ=255)
                                                       gTGCTTCCCGCCCTTCGCGTATCAATTGCGGGTTGGCCAGAACGGCAGCAGTAAGGCGATCGCGGTAGTAACGATCCACCCAGTCATTGAGCGCATTa                                          <  1:1135205/98‑1 (MQ=255)
                                                        tGCTTCCCGCCCTTCGCGCAGCAATTGCGGGTCGGCCAGAACGGCAGCAGTAAGGCGATCGCGGTAGTAACGATCCACCCAGTCATTGAGCGCATTAAAc                                       <  1:2764166/100‑1 (MQ=255)
                                                         ncTTCCCGCCCTTCGCGCAACAATTGCGAGTCGGCCAGAACGGCAGCAGTAAGGCGATCGCGGTAGTAACGATCCACCCAGTCATGGAGCGCATTAAACa                                      >  2:653913/2‑100 (MQ=255)
                                                          cTTCCCGCTCTTCGCGCAGCAATTGCGGGTCGGCCAGAACGGCAGCAGTAAGGCGATCGCGGTAGTAACGATCCACCCAGTCATTGAGCGCATTAAACAg                                     <  1:621140/100‑1 (MQ=255)
                                                          cTTCCCGCCCTTCGCGCAGCAATTGCGGGTCGGCCAGAACGGCAGCAGTAAGGCGATCGCGGTAGTAACGATCCACCCAGTCATTGAGCGCATTAAACAg                                     >  1:930365/1‑100 (MQ=255)
                                                          cTTCCCGCCCTTCGCGCAGCAATTGCGGGTCGGCCAGAACGGCAGCAGTAAGGCGATCGCGGTAGTAACGATCCACCCAGTCATTGAGCGCATTAAACAg                                     >  2:42230/1‑100 (MQ=255)
                                                          cTTCCCGCCCTTCGCGCAGCAATTGCGGGTCGGCCAGAACGGCAGCAGTAAGGCGATCGCGGTAGTAACGATCCACCCAGTCATTGAGCGCATTAAACAg                                     >  1:216689/1‑100 (MQ=255)
                                                           ttCCCGCCCTTCGCGCAGCAATTGCGGGTCGGCCAGAACGGCAGCAGTAAGGCGATCGCGGTAGTAACGATCCACCCAGTCATTGAGCGCATTAAACAGc                                    <  1:1499064/100‑1 (MQ=255)
                                                            tCCCGCCCTTCGCGCAGCAATTGCGGGTCGGCCAGAACGGCAGCAGTAAGGCGATCGCGGTAGTAACGATCCACCCAGTCATTGAGCGCATTAAACAGCg                                   <  1:204565/100‑1 (MQ=255)
                                                            tCCCGCCCTTCGCGCAGCAATTGCGGGTCGGCCAGAACGGCAGCAGTAAGGCGATCGCGGTAGTAACGATCCACCCAGTCATTGAGCGCATTAAACAGCg                                   >  2:204563/1‑100 (MQ=255)
                                                                  ccTTCGCGCAGCAATTGCGGGTCGGCCAGAACGGCAGCAGTAAGGCGATCGCGGTAGTAACGATCCACCCAGTCATTGAGCGCATTAAACAGCGTATCGt                             >  2:2082030/1‑100 (MQ=255)
                                                                  ccTTCGCGCAGCAATTGCGGGTCGGCCAGAACGGCAGCAGTAAGGCGATCGCGGTAGTAACGATCCACCCAGTCATTGAGCGCATTAAACAGCGTATCGt                             >  2:330063/1‑100 (MQ=255)
                                                                     tCGCGCAGCAATTGCGGGTCGGCCAGAACGGCAGCAGTAAGGCGATCGCGGTAGTAACGATCCACCCAGTCATTGAGCGCATTAAACAGCGTATCGTtca                          <  2:84475/100‑1 (MQ=255)
                                                                      cgcgCAGCAATTGCGGGTCGGCCAGAACGGCAGCAGTAAGGCGATCGCGGTAGTAACGATCCACCCAGTCATTGAGCGCATTAAACAGCGTATCGTtcat                         >  1:1018366/1‑100 (MQ=255)
                                                                      cgcgCAGCAATTGCGGGTCGGCCAGAACGGCAGCAGTAAGGCGATCGCGGTAGTAACGATCCACCCAGTCATTGAGCGCATTAAACAGCGTATCGTtcat                         >  2:1916352/1‑100 (MQ=255)
                                                                      cgcgCAGCAATTGCGGGTCGGCCAGAACGGCAGCAGTAAGGCGATCGCGGTAGTAACGATCCACCCAGTCATTGAGCGCATTAAACAGCGTATCGTtcat                         <  1:1270097/100‑1 (MQ=255)
                                                                      cgcgCAGCAATTGCGGGTCGACCAGAACGGCAGCAGTAAGGCGATCGCGGTAGTAACGATCCACCCAGTCATTGAGCGCATTAAACAGCGTATCGTtcat                         <  1:22955/100‑1 (MQ=255)
                                                                        cgcAGCAATTGCGGGTCGGCCAGAACGGCAGCAGTAAGGCGATCGCGGTAGTAACGATCCACCCAGTCATTGAGCGCATTAAACAGCGTATCGTtcatca                       <  2:216687/100‑1 (MQ=255)
                                                                        cgcAGCAATTGCGGGTCGGCCAGAACGGCAGCAGTAAGGCGATCGCGGTAGTAACGATCCACCCAGTCATTGAGCGCATTAAACAGCGTATCGTtcatca                       <  2:930359/100‑1 (MQ=255)
                                                                        cgcAGCAATTGCGGGTCGGCCAGAACGGCAGCAGTAAGGCGATCGCGGTAGTAACGATCCACCCAGTCATTGAGCGCATTAAACAGCGTATCGTtcatca                       <  1:2098153/100‑1 (MQ=255)
                                                                        cgcAGCAATTGCGGGTCGGCCAGAACGGCAGCAGTAAGGCGATCGCGGTAGTAACGATCCACCCAGTCATTGAGCGCATTAAACAGCGTATCGTtcatca                       >  1:493469/1‑100 (MQ=255)
                                                                         gcagcaATTGCGGGTCGGCCAGAACGTCGGCAATAAGGCTATCGCGGTAGTTACGATGCACCCAGTCATTGAGCGAATTAAACAGCGTATCGTtc                           <  2:1186433/95‑1 (MQ=255)
                                                                          cagcaATTGCGGGTCGGCCAGAACGGCAGCAGTAAGGCGATCGCGGTAGTAACGATCCACCCAGTCATTGAGCGCATTAAACAGCGTATCGTtcatcatc                     >  2:139436/1‑100 (MQ=255)
                                                                            gcaATTGCGGGTCGGCCAGATCGGCAGCAGTAAGGCGATCGCGGTAGTAACGATCCACCCAGACATTGAGCGCATTAAACAGCGTATCGTTCATCATCAc                   >  1:2409526/1‑100 (MQ=255)
                                                                            gcaATTGCGGGTCGGCCAGAACGGCAGCAGTAAGGCGATCGCGGTAGTAACGATCCACCCAGTCATTGAGCGCATTAAACAGCGTATCGTTCATCATCAc                   <  2:2207257/100‑1 (MQ=255)
                                                                               aTTGCGGGTCGGCCAGAACGGCAGCAGTAAGGCGATCGCGGTAGTAACGATCCACCCAGTCATTGAGCGCATTAAACAGCGTATCGTTCATCATCAccgc                >  2:1009842/1‑100 (MQ=255)
                                                                                ttGCGGGTCGGCCAGAACGGCAGCAGTAAGGCGATCGCGGTAGTAACGATCCACCCAGTCATTTAGCGCATTAAACAGCGTATCGTTCATCATCAccgcc               <  1:2137865/100‑1 (MQ=255)
                                                                                 tGCGGGTCGGCCAGAACGGCAGCAGTAAGGCGATCGCGGTAGTAACGATCCACCCAGTCATTGAGCGCATTAAACAGCGTATCGTTCATCATCAccgccg              <  2:1432876/100‑1 (MQ=255)
                                                                                 tGCGGGTCGGCCAGAACGGCAGCAGTAAGGCGATCGCGGTAGTAACGATCCACCCAGTCATTGAGCGCATTAAACAGCGTATCGTTCATCATCAccgccg              <  2:622463/100‑1 (MQ=255)
                                                                                  gCGGGTCGGCCAGAACGGCAGCAGTAAGGCGATCGCGGTAGTAACGATCCACCCAGTCATTGAGCGCATTAAACAGCGTATCGTTCATCATCACCGCCgg             <  1:2386118/100‑1 (MQ=255)
                                                                                    gggTCGGCCAGAACGGCAGCAGTAAGGCGATCGCGGTCGTAACGATCCACCCAGTCATTGAGCGCATTAAACAGCGCATCGTTCATCATCAccgcc               >  2:94765/1‑96 (MQ=255)
                                                                                    gggTCGGCCAGAACGGCAGCAGTAAGGCGATCGCGGTAGTAACGATCCACCCAGTCATTGAGCGCATTAAACAGCGTATCGTTCATCATCACCGCCGGAt           >  1:327641/1‑100 (MQ=255)
                                                                                     ggTCGGCCAGAACGGCAGCAGTAAGGCGATCGCGGTAGTAACGATCCACCCAGTCATTGAGCGCATTAAACAGCGTATCGTTCATCATCACCGCCGGAtt          >  1:2657556/1‑100 (MQ=255)
                                                                                      gTCGGCCAGAACGGCAGCAGTAAGGCGATCGCGGTAGTAACGATCCACCCAGTCATTGAGCGCATTAAACAGCGTATCGTTCATCATCACCGCCGGATTc         <  2:2913149/100‑1 (MQ=255)
                                                                                      gTCGGCCAGAACGGCAGCAGTAAGGCGATCGCGGTAGTAACGATCCACCCAGTCATTGAGCGCATTAAACAGCGTATCGTTCATCATCACCGCCGGATTc         >  1:642909/1‑100 (MQ=255)
                                                                                        cGGCCAGAACGGCAGTAGTAAGGCGATCGCGGTAGTAACGATCCACCCAGTCATTGAGCGCATTAAACAGCGTATCGTTCATCATCACCGCCgggcggtg       <  1:215922/100‑7 (MQ=255)
                                                                                        cGGCCAGAACGGCAGCAGTAAGGCGATCGCGGTAGTAACGATCCACCCAGTCATTGAGCGCATTAAACAGCGTATCGTTCATCATCACCGCCgggcggtg       >  1:2144098/1‑94 (MQ=255)
                                                                                         ggCCAGAACGGCAGCAGTAAGGCGATCGCGGTAGTAACGATCCACCCAGTCATTGAGCGCATTAAACAGCGTATCGTTCATCATCACCGCCGGATTCAcc      <  2:1130159/100‑1 (MQ=255)
                                                                                          gCCAGAACGGCAGCAGTAAAGCGATCGCGGTAGTAACGATCCACCCAGTCATTGAGCGCATTAAACAGCGTATCGTTCATCGTCACCGCCGGATTCAc       >  2:1573802/1‑98 (MQ=255)
                                                                                           ccAGAACGGCAGCAGTAAGGCGATCGCGGTAGTAACGATCCACCCAGTCATTGAGCGCATTAAACAGCGTATCGTTCATCATCACCGCCGGATTCACCGc    <  1:2274262/100‑1 (MQ=255)
                                                                                           ccAGAACGGCAGCAGTAAGGCGATCGCGGTAGTAACGATCCACCCAGTCATTGAGCGCATTAAACAGCGTATCGTTCATCATCACCGCCGGATTCACCGc    >  2:2417750/1‑100 (MQ=255)
                                                                                             aGAACGGCAGCAGTAAGGCGATCGCGGTAGTAACGATCCACCCAGTCATTGAGCGCATTAAACAGCGTATCGTTCATCATCACCGCCGGATTCACCGccc  <  1:494538/100‑1 (MQ=255)
                                                                                                |                                                                                                
TCGCGCTGGAACGGATAAACCGAACCGAGATTCAGTAATTGGCTCAATACATCCAGTGCTTCCCGCCCTTCGCGCAGCAATTGCGGGTCGGCCAGATCGGCAGCAGTAAGGCGATCGCGGTAGTAACGATCCACCCAGTCATTGAGCGCATTAAACAGCGTATCGTTCATCATCACCGCCGGATTCACCGCCC  >  NC_000913/1826935‑1827127

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: