Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,983,761 C→T 100% E437K (GAA→AAA)  araG ← L‑arabinose ABC transporter ATPase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,983,7610CT97.0% 317.2 / ‑0.3 101E437K (GAA→AAA) araGL‑arabinose ABC transporter ATPase
Reads supporting (aligned to +/- strand):  ref base C (2/1);  new base T (40/58);  total (42/59)
Fisher's exact test for biased strand distribution p-value = 5.69e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.22e-01

Only 100 of 103 total aligned reads displayed.

ATCCGGTCGGCAACGCCGAGGACTTCAGGTAAGTCGCTGGAGGCAAACAGCACCGCCACGCCCTGCGCCGCCAGCGCATAAATTACGTTATATATTTCGTGCTTAGCGCCAACATCAATGCCGCGCGTAGGTTCATCCAGCAAAATGACCTTCATCTCTTCCGATAACCAGCGGCCCAGAATGGCTTTTTGCT  >  NC_000913/1983664‑1983856
                                                                                                 |                                                                                               
aTCCGGTCGGCAACGCCGAGGACTTCAGGTAAGTCGCTGGAGGCAAACAGCACCGCCACGCCCTGCGCCGCCAGCGCATAAATTACGTTATATATTTTGt                                                                                               <  2:1445274/100‑1 (MQ=255)
 tCCGGTCGGCAACGCCGAGGACTTCAGGTAAGTCGCTGGAGGCAAACAGCACCGCCACGCCCTGCGCCGCCAGCGCATAAATTACGTTATATATTTtgtg                                                                                              <  2:2185001/100‑1 (MQ=255)
  ccGGTCGGCAACGCCGAGGACTTCAGGTAAGTCGCTGGAGGCAAACAGCACCGCCACGCCCTGCGCCGCCAGCGCATAAATTACGTTATATATTTtgtg                                                                                              <  2:646105/99‑1 (MQ=255)
  ccGGTCGGCAACGCCGAGGACTTCAGGTAAGTCGCTGGAGGCAAACAGCACCGCCACGCCCTGCGCCGCCAGCGCATAAATTACGTTATATATTTTGTGc                                                                                             >  2:2644650/1‑100 (MQ=255)
   cGGTCGGCAACGCCGAGGACTTCAGGTAAGTCGCTGGAGGCAAACAGCACCGCCACGCCCTGCGCCGCCAGCGAATAAATTACGTTATATATTTTGTGCt                                                                                            >  1:1820159/1‑100 (MQ=255)
    ggTCGGCAACGCCGAGGACTTCAGGTAAGTCGCTGGAGGCAAACAGCACCGCCACGCCCTGCGCCGCCAGCGCATAAATTACGTTATATATTTTGTGCtt                                                                                           >  1:2700340/1‑100 (MQ=255)
    ggTCGGCAACGCCGAGGACTTCAGGTAAGTCGCTGGAGGCAAACAGCACCGCCACGCCCTGCGCCGCCAGCGCATAAATTACGTTATATATTTTGTGCtt                                                                                           <  1:2191521/100‑1 (MQ=255)
     gTCGGCAACGCCGAGGACTTCAGGTAAGTCGCTGGAGGCAAACAGCACCGCCACGCCCTGCGCCGCCAGCGCATAAATTACGTTATATATTTTGTGCTTa                                                                                          >  1:1826075/1‑100 (MQ=255)
     gTCGGCAACGCCGAGGACTTCAGGTAAGTCGCTGGAGGCAAACAGCACCGCCACGCCCTGCGCCGCCAGCGCATAAATTACGTTATATATTTTGTGCTTa                                                                                          <  2:388751/100‑1 (MQ=255)
      tCGGCAACGCCGAGGACTTCAGGTAAGTCGCTGGAGGCAAACAGCACCGCCACGCCCTGCGCCGCCAGCGCATAAATTACGTTATATATTTTGTGCTTAg                                                                                         <  1:2905980/100‑1 (MQ=255)
       cGGCAACGCCGAGGACTTCAGGTAAGTCGCTGGAGGCAAACAGCACCGCCACGCCCTGCGCCGCCAGCGCATAAATTACGTTATATATTTTGTGCTTAgc                                                                                        <  1:1592035/100‑1 (MQ=255)
        ggCAACGCCGAGGACTTCAGGTAAGTCGCTGGAGGCAAACAGCACCGCCACGCCCTGCGCCGCCAGCGCATAAATTACGTTATATATTTTGTGCTTAgcg                                                                                       <  1:859067/100‑1 (MQ=255)
         gccaCGCCGAGGACTTCAGGTAAGTCGCTGGAGGCAAACAGCACCGTCACGCCCTGCGCCGCCAGCGCATAAATTACGTTGTATATTTTGTGCTTAgcgc                                                                                      <  2:653535/97‑1 (MQ=255)
         gCAACGCCGAGGTCTTCAGGTAAGTCGCTGAAGGCAAACAGCACCGCCACGCCCTGCGCCGCCAGCGCATAAATTACGCTATATATGTTGTGCTTAgcgc                                                                                      >  2:1374311/1‑100 (MQ=255)
         gCAACGCCGAGGACTTCAGGTAAGTCGCTGGAGGCAAACAGCACCGCCACGCCCTGCGCCGCCAGCGCATAAATTACGTTATATATTTTGTGCTTAgcgc                                                                                      >  2:680875/1‑100 (MQ=255)
         gCAACGCCGAGGACTTCAGGTAAGTCGCTGGAGGCAAACAGCACCGCCACGCCCTGCGCCGCCAGCGCATAAATTACGTTATATATTTTGTGCTTAgcgc                                                                                      >  2:2112000/1‑100 (MQ=255)
         gCAACGCCGAGGACTTCAGGTAAGTCGCTGGAGGCAAACAGCACCGCCACGCCCTGCGCCGCCAGCGCATAAATTACGTTATATATTTTGTGCTTAgcgc                                                                                      <  2:2999857/100‑1 (MQ=255)
            aCGCCGAGGACTTCAGGTAAGTCGCTGGAGGCAAACAGCACCGCCACGCCCTGCGCCGCCAGCGCATAAATTACGTTATATATTTTGTGCTTAGCGCCaa                                                                                   <  2:1342620/100‑1 (MQ=255)
            aCGCCGAGGACTTCAGGTAAGTCGCTGGAGGCAAACAGCACCGCCACGCCCTGCGCCGCCAGCGCATAAATTACGTTATATATTTTGTGCTTAGCGCCaa                                                                                   <  1:672268/100‑1 (MQ=255)
             cGCCGAGGACTTCAGGTAAGTCACTGGAGGCAAACAGCACCGCCACGCCCTGCGCCGCCAGCGCATAAATTACGTTATATATTTTGTGCTTAGCGCCAAc                                                                                  <  1:572611/100‑1 (MQ=255)
             cGCCGAGGACTTCAGGCAAGTCGCTGGAGGCAAACAGCACCGCCACGCCCTGCGCCGCCAGCGCATAAATTACGTTATATATTTTGTGCTTAGCGCCAAc                                                                                  >  2:2900792/1‑100 (MQ=255)
              gCCGAGGACTTCAGGTAAGTCGCTGGAGGCAAACAGCACCGCCACGCCCTGCGCCGCCAGCGCATAAATTACGTTATATATTTTGTGCTTAGCGCCAACa                                                                                 <  1:2238112/100‑1 (MQ=255)
               ncGAGGACTTCAGGTAAGTCGCTGGAGGCAAACAGCACCGCCACGCCCTGCGCCGCCAGCGCATAAATTACGTTATATATTTTGTGCTTAGCGCCAACAt                                                                                >  1:2768475/2‑100 (MQ=255)
               ccGAGGACTTCAGGTAAGTCGCTGGAGGCAAACAGCACCGCCACGCCCTGCGCCGCCAGCGCATAAATTACGTTATATATTTTGTGCTTAGCGCCAACAt                                                                                >  2:2320361/1‑100 (MQ=255)
                 gAGGACTTCAGGTAAGTCGCTGGAGGCAAACAGCACCGCCACGCCCTGCGCCGCCAGCGCATAAATTACGTTATATATTTTGTGCTTAGCGCCAACATCa                                                                              >  2:2196728/1‑100 (MQ=255)
                     aCTTCAGGTAAGTCGCTGGAGGCAAACAGCACCGCCATGCACTGCGCCGCCAGCGCATAAATTACGTTATATATTTTGTGCTTAGCGCCAACATCAATGc                                                                          <  1:1197535/100‑1 (MQ=255)
                     aCTTCAGGTAAGTCGCTGGAGGCAAACAGCACCGCCACGCCCTGCGCCGCCAGCGCATAAATTACGTTATATATTTTGTGCTTAGCGCCAACATCAATGc                                                                          <  2:663719/100‑1 (MQ=255)
                      cTTCAGGTAAGTCGCTGGAGGCAAACAGCACCGCCACGCCCTGCGCCGCCAGCGCATAAATTACGTTATATATTTTGTGCTTAGCGCCAACATCAATGcc                                                                         >  1:2347280/1‑100 (MQ=255)
                       ttCAGGTAAGTCGCTGGAGGCAAACAGCACCGCCACGCCCTGCGCCGCCAGCGCATAAATTACGTTATATATTTTGTGCTTAGCGCCAACATCAATGCcg                                                                        <  1:1280535/100‑1 (MQ=255)
                         cAGGTAAGTCGCTGGAGGCAAACAGCACCGCCACGCCCTGCGCCGCCAGCGCATAAATTACGTTATATATTTTGTGCTTAGCGCCAACATCAATGCcgcg                                                                      <  2:2038734/100‑1 (MQ=255)
                         cAGGTAAGTCGCTGGAGGCAAACAGCACCGCCACGCCCTGCGCCGCCAGCGCATAAATTACGTTATATATTTTGTGCTTAGCGCCAACATCAATGCcgcg                                                                      <  1:1007061/100‑1 (MQ=255)
                           ggTAAGTCGCTGGAGGCAAACAGCACCGCCACGCCCTGCGCCGCCAGCGCATAAATTACGTTATATATTTTGTGCTTAGCGCCAACTTCAATGCcgcgcg                                                                    <  1:2044697/100‑1 (MQ=255)
                                 tCGCTGGAGGCAAACAGCACCGCTACGCCTTGCGCCGTCAGCGCATAAATTACGTTATATATTTTGTGCTTAGCGCCAACATCAATGCCGCGCGTAGGtt                                                              <  1:1582637/100‑1 (MQ=255)
                                 tCGCTGGAGGCAAACAGCACCGCCACGCCCTGCGCCGCCAGCGCATAAATTACGTTATATATTTTGTGCTTAGCGCCAACATCTATGCCGCGCGTAGGtt                                                              <  2:2484056/100‑1 (MQ=255)
                                 tCGCTGGAGGCAAACAGCACCGCCACGCCCTGCGCCGCCAGCGCATAAATTACGTTATATATTTTGTGCTTAGCGCCAACATCAATGCCGCGCGTAGGtt                                                              <  1:2387802/100‑1 (MQ=255)
                                  cGCTGGAGGCAAACAGCACCGCCACGCCCTGCGCCGCCAGCGCATAAATTACGTTATATATTTTGTGCTTAGCGCCAACATCAATGCCGCGCGTAGGTTc                                                             <  1:2718735/100‑1 (MQ=255)
                                  cGCTGGAGGCAAACAGCACCGCCACGCCCTGCGCCGCCAGCGCATAAATTACGTTATATATTTTGTGCTTAGCGCCAACATCAATGCCGCGCGTAGGTTc                                                             <  2:2836553/100‑1 (MQ=255)
                                       gAGGCAAACAGCACCGCCACGCCCTGCGCCGCCAGCGCATAAATTACGTTATATATTTTGTGCTTAGCGCCAACATCAATGCCGCGCGTAGGTTCATCCa                                                        <  2:1732581/100‑1 (MQ=255)
                                       gAGGCAAACAGCACCGCCACGCCCTGCGCCGCCAGCGCATAAATTACGTTATATATTTTGTGCTTAGCGCCAACATCAATGCCGCGCGTAGGTTCATCCa                                                        <  1:1121366/100‑1 (MQ=255)
                                       gAGGCAAACAGCACCGCCACGCCCTGCGCCGCCAGCGCATAAATTACGTTATATATTTTGTGCTTAGCGCCAACATCAATGCCGCGCGTAGGTTCATCCa                                                        <  2:2692036/100‑1 (MQ=255)
                                        aGGCAAACAGCACCGCCACGCCCTGCGCCGCCAGCGCATAAATTACGTTATATATTTTGTGCTTAGCGCCAACATCAATGCCGCGCGTAGGTTCATCCAg                                                       >  2:2860297/1‑100 (MQ=255)
                                         ngCAAACAGCACCGCCACGCCCTGCGCCGCCAGCGCATAAATTACGTTATATATTTTGTGCTTAGCGCCAACATCAATGCCGCGCGTAGGTTCATCCAGc                                                      >  1:2385103/2‑100 (MQ=255)
                                         ggCAAACAGCACCGCCACGCCCTGCGCCGCCAGCGCATAAATTCCGTTATATATTTTGTGCTTAGCGCCAACATCAATGCCGCGCGTAGGTCCATCCAGc                                                      >  2:2500494/1‑100 (MQ=255)
                                          gCAAACAGCACCGCCACGCCCTGCGCCGCCAGCGCATAAATTACGTTATATATTTTGTGCTTAGCGCCAACATCAATGCCGCGCGTAGGTTCATCCAGCa                                                     <  1:1648027/100‑1 (MQ=255)
                                           naaaCAGCACCGCCACGCCCTGCGCCGCCAGCGCAAAAACACCATTATATATCTTGTGCTTAGCGCCAACATCAATGCCGCGCGGAGGTTCATCCAGCaa                                                    >  2:2075973/2‑100 (MQ=255)
                                           cAAACAGCACCGCCACGCCCTGCGCCGCCAGCGCATAAATTACGTTATATATTTTGTGCTTAGCGCCAACATCAATGCCGCGCGTAGGTTCATCCAGCaa                                                    >  2:61530/1‑100 (MQ=255)
                                           cAAACAGCACCGCCACGCCCTGCGCCGCCAGCGCATAAATTACGTTATATATTTTGTGCTTAGCGCCAACATCAATGCCGCGCGTAGGTTCATCCAGCaa                                                    >  2:2406614/1‑100 (MQ=255)
                                             aaCAGCACCGCCACGCCCTGCGCCGCCAGCGCATAAATTACGTTATATATTTTGTGCTTAGCGCCAACATCAATGCCGCGCGTAGGTTCATCCAGCaaaa                                                  <  1:2829197/100‑1 (MQ=255)
                                               cAGCACCGCCACGCCCTGCGCCGCCAGCGCATAAATTACGTTATATATTTTGTGCTTAGCGCCAACATCAATGCCGCGCGTAGGTTCATCCAGCAAAATg                                                >  2:1508106/1‑100 (MQ=255)
                                                aGCACCGCCTCGCCCTGCGCCGCCAGCGCATAAATTACGTTATATATTTTGTGCTTAGCGCCAACATCAATGCCGCGCGTAGGTTCATCCAGCAAAATg                                                <  1:1446000/99‑1 (MQ=255)
                                                aGCACCGCCACGCCCTGCGCCGCCAGCGCATAAATTACGTTATATATTTTGTGCTTAGCGCCAACATCAATGCCGCGCGTAGGTTCATCCAGCAAAATGa                                               >  2:278577/1‑100 (MQ=255)
                                                  cACCGCCACGCCCTGCGCCGCCAGCGCATAAATTACGTTATATATTTTGTGCTTAGCGCCAACATCAATGCCGCGCGTAGGTTCATCCAGCAAACTGAcc                                             >  2:3022503/1‑100 (MQ=255)
                                                  cACCGCCACGCCCTGCGCCGCCAGCGCATAAATTACGTTATATATTTTGTGCTTAGCGCCAACATCAATGCCGCGCGTAGGTTCATCCAGCAAAATGAcc                                             >  1:1615056/1‑100 (MQ=255)
                                                  cACCGCCACGCCCTGCGCCGCCAGCGCATAAATTACGTTATATATTTTGTGCTTAGCGCCAACATCAATGCCGCGCGTAGGTTCATCCAGCAAAATGAcc                                             <  1:986661/100‑1 (MQ=255)
                                                    ccGCCACGCCCTGCGCCGCCAGCGCATAAATTACGTTATATATTTTGTGCTTAGCGCCAACATCAATGCCGCGCGTAGGTTCATCCAGCAAAATGACCtt                                           <  1:2282007/100‑1 (MQ=255)
                                                    ccGCCACGCCCTGCGCCGCCAGCGCATAAATTACGTTATATATTTTGTGCTTAGCGCCAACATCAATGCCGCGCGTAGGTTCATCCAGCAAAATGACCtt                                           >  2:2310248/1‑100 (MQ=255)
                                                    ccGCCACGCCCTGCGCCGCCAGCGCATAAATTACGTTATATATTTTGTGCTTAGCGCCAACATCAATGCCGCGCGTAGGTTCATCCAGCAAAATGACCtt                                           <  1:1457880/100‑1 (MQ=255)
                                                    ccGCCACGCCCTGCGCCGCCAGCGCATAAATTACGTTATATATTTCGTGCTTAGCGCCAACATCAATGCCGCGCGTAGGTTCATCCAGCAAAATGACCtt                                           >  2:1378778/1‑100 (MQ=255)
                                                      gCCACGCCCTGCGCCGCCAGCGCATAAATTACGTTATATATTTTGTGCTTAGCGCCAACATCAATGCCGCGCGTAGGTTCATCCAGCAAAATGACCTTCa                                         >  1:571603/1‑100 (MQ=255)
                                                      gCCACGCCCTGCGCCGCCAGCGCATAAATTACGTTATATATTTTGTGCTTAGCGCCAACATCAATGCCGCGCGTAGGTTCATCCAGCAAAATGACCTTCa                                         >  1:2666283/1‑100 (MQ=255)
                                                      cgtaatttatGCGCTGGCGGCGCATAAATTACGTTATATATTTTGTGCTTAGCGCCAACATCAATGCAGCACGTAAGTTCATCCAGCAAAAAGACCTTCa                                         >  1:2016705/10‑100 (MQ=255)
                                                       ccACGCCCTGCGCCGCCAGCGCATAAATTACGTTATATATTTTGTGCTTAGCGCCAACATCAATGCAGCGCGTAGGTTCATCCAGCAAAATGACCTTCAt                                        >  1:2695273/1‑100 (MQ=255)
                                                       ccACGCCCTGCGCCGCCAGCGCATAAATTACGTTATATATTTCGTGCTTAGCGCCAACATCAATGCCGCGCGTAGGTTCATCCAGCAAAATGACCTTCAt                                        >  1:1453985/1‑100 (MQ=255)
                                                        cACGCCCTGCGCCGCCAGCGCATAAATTTCGTTATATATTTCGTGCTTAGCGCCAACATCAATGCCGCGCGTAGGTTCATCCAGCAAAATGACCTTCAtc                                       <  1:1378791/100‑1 (MQ=255)
                                                         aCGCCCTGCGCCGCCAGCGCATAAATTACGTTATATATTTTGTGCTTAGCGCCAACATCAATGCCGCGCGTAGGTTCATCCAGCAAAATGACCTTCAtct                                      >  2:2029872/1‑100 (MQ=255)
                                                         aCGCCCTGCGCCGCCAGCGCATAAATTACGTTATATATTTTGTGCTTAGCGCCAACATCAATGCCGCGCGTAGGTTCATCCAGCAAAATGACCTTCAtct                                      <  1:2900824/100‑1 (MQ=255)
                                                         aCGCCCTGCGCCGCCAGCGCATAAATTACGTTATATATTTTGTGCTTAGCGCCAACATCAATGCCGCGCGTAGGTTCATCCAGCAAAATGACCTTCAtc                                       <  1:609278/99‑1 (MQ=255)
                                                          cGCCCTGCGCCGCCAGCGCATAAATTACGTTATATTTTTTGTGCTTAGCGCCAACATCAATGCCGCGCGTAGGTTCATCCAGCAAAATGACCTTCAtctc                                     <  1:951750/100‑1 (MQ=255)
                                                          cGCCCTGCGCCGCCAGCGCATAAATTACGTTATATATTTTGTGCTTAGCGCCAACATCAATGCCGCGCGTAGGTTCATCCAGCAAAATGACCTTCAtctc                                     <  2:334958/100‑1 (MQ=255)
                                                           tttatGCGCCGCCAGCGCATAAATTACGTTATATATTTTGTGCTTAGCGCCAACATCAATGCCGCGCGTAGGTTCATCCAGCAAAATGACCTTCATctct                                    >  2:1442432/5‑100 (MQ=255)
                                                            cccTGCGCCGCCAGCGCATAAATTACGTTATATATTTTGTGCTTAGCGCCAACATCAATGCCGCGCGTAGGTTCATCCAGCAAAATGACCTTCATCTCtt                                   >  1:619777/1‑100 (MQ=255)
                                                            cccTGCGCCGCCAGCGCATAAATTACGTTATATATTTTGTGCTTAGCGCCAACATCAATGCCGCGCGTAGGTTCATCCAGCAAAATGACCTTCATCTCtt                                   >  1:2280461/1‑100 (MQ=255)
                                                              cTGCGCCGCCAGCGCATAAATTACGTTATATATTTTGTGCTTAGCGCCAACATCAATGCCGCGCGTAGGTTCATCCAGCAAAATGACCTTCATCTCTTcc                                 <  1:1414111/100‑1 (MQ=255)
                                                              cTGCGCCGCCAGCGCATAAATTACGTTATATATTTTGTGCTTAGCGCCAACATCAATGCCGCGCGTAGGTTCATCCAGCAAAATGACCTTCATCTCTTcc                                 <  1:396149/100‑1 (MQ=255)
                                                              cTGCGCCGCAAGCGGATAAATTACGTTATATATTTTGTGCTTAGCGACAACATCAATGCCGCGCGTAGGTTCATCCATCAAAATGACCTTCATCTCTTcc                                 <  2:741874/100‑1 (MQ=255)
                                                               tGCGCCGCCAGCGAATAAATTACGTTATATATTTTGAAATTAGCGCTAACATCAATGCGGCGCGTAGGTTCATCCAGCAAAATGACCTTCATCACTACCg                                >  2:1214138/1‑100 (MQ=255)
                                                                gcgcCGCCAGCGCATAAATTACGTTATATATTTTGTGCTTAGCGCCAACATCAATGCCGCGCGTAGGTTCATCCAGCAAAATGACCTTCATCTCTTCCGa                               <  1:2860329/100‑1 (MQ=255)
                                                                   ccgccAGCGCATAAATTACGTTATATATTTTGTGCTTATCGCCAACATCAATGCCGCGCGGAGGTTCAGCCAGCAAAATGACCTTCATCTCTTCCGATaa                            <  1:2876730/100‑1 (MQ=255)
                                                                        aGCGCATAAATTACGTTATATATTTTGTGCTTAGCGCCAACATCAATGCCGCGCGTAGGTTCATCCAGCAAAATGACCTTCATCTCTTCCGATAACCAGc                       <  1:1664710/100‑1 (MQ=255)
                                                                         gcgcATAAATTACGTTATATATTTTGTGCTTAGCGCCAACATCAATGCCTCGCGTATGTTCATCCAGCAATATTACCTTCATTTCGTCCAATAACCAGCg                      <  2:362681/100‑1 (MQ=255)
                                                                            cATAAATTTCGTTATATATTTTGTGCTTAGCGCCAACATCAATGCCGCGCGTAGGTTCATCCAGCAAAATGACCTTCATCTCTTCCGATAACCAGCGGc                    <  1:2726029/99‑1 (MQ=255)
                                                                             aTAAATTACGTTATATATTTTGTGCTTAGCGCCAACATCAATGCCGCGCGTAGGTTCATCCAGCAAAATGACCTTCATCTCTTCCGATAACCAGCGGccc                  >  2:1733073/1‑100 (MQ=255)
                                                                             aTAAATGACGTTATATATTTTGTGCTTAGCGGCAACGTCAATGACACGCGTCGGGTGATCCAGTAAAATGACCTTAATCTCTTCCGATAACCa                         <  2:2851193/93‑1 (MQ=255)
                                                                              tAAATTACGTTATATATTTTGTGCTTAGCGCCAACATCAATGCCGCGCGTAGGTTCATCCAGCAAAATGACCTTCATCTCTTCCGATAACCAGCGGCCCa                 <  1:1508123/100‑1 (MQ=255)
                                                                              tAAATTACGTTATATATTTTGTGCTTAGCGCCAACATCAATGCCGCGCGTAGGTTCATCCAGCAAAATGACCTTCATCTCTTCCGATAACCAGCGGCCCa                 <  1:2277156/100‑1 (MQ=255)
                                                                               aaaTTACGTTATATATTTTGTGCTTAGCGCCAACATCAATGCCGCGCGTAGGTTCATCCAGCAAAATGACCTTCATCTCTTCCGATAACCAGCGGCCCAg                <  1:1442446/100‑1 (MQ=255)
                                                                                 aTTACGTTATATATTTTGTGCTTAGCGACAAAATCAATGCAGCGCGTAGGTTCATCCAGCAAAATGACCTTCATCTCTTCCGATAACAAGCGGCCCAGaa              >  2:1445219/1‑100 (MQ=255)
                                                                                  ttACGTTATATATTTTGTGCTTAGCGCCAACATCAATGCCGCGCGTAGGTTCATCCAGCAAAATGACCTTCATCTCTTCCGATAACCAGCGGCCCAGAAt             >  1:2727998/1‑100 (MQ=255)
                                                                                   tACGTTATATATTTTGTGCTTAGCGCCAACATCAATGCCGCGCGTAGGTTCATCCAGCAAAATGACCTTCATCTCTTCCGATAACCAGCGGCCCAGAATg            <  2:1727611/100‑1 (MQ=255)
                                                                                   tACGTTATATATTTTGTGCTTAGCGCCAACATCAATGCCGCGCGTAGGTTCATCCAGCAAAATGACCTTCATCTCTTCCGATAACCAGCGGCCCAGAATg            <  2:2421801/100‑1 (MQ=255)
                                                                                      gTTATATATTTTGTGCTTAGCGCCAACATCAATGCCGCGCGTAGGTTCATCCAGCAAAATGACCTTCATCTCTTCCGATAACCAGCGGCCCAGAATGGCt         <  1:1264443/100‑1 (MQ=255)
                                                                                       ttATATATTTTGTGCTTAGCGCCAACATCAATGCCGCGCGTAGGTTCATCCAGCAAAATGACCTTCATCTCTTCCGATAACCAGCGGCCCAGAATGGCtt        >  1:3046568/1‑100 (MQ=255)
                                                                                       ttATATATTTTGTGCTTAGCGCCAACATCAATGCCGCGCGTAGGTTCATCCAGCAAAATGACCTTCATCTCTTCCGATAACCAGCGGCCCAGAATGGCtt        <  1:1816191/100‑1 (MQ=255)
                                                                                       ttATATATTTTGTGCTTAGCGCCAACATCAATGCCGCGCGTAGGTTCATCCAGCAAAATGACCTTCATCTCTTCCGATAACCAGCGGCCCAGAATGGCtt        >  1:3046977/1‑100 (MQ=255)
                                                                                        tatataTTTTGTGCTTAGCGCCAACATCAATGCCGCGCGTAGGTTCATCCAGCAAAATGACCTTCATCTCTTCCGATAACCAGCGGCCCAGAATGGCttt       >  1:1111999/1‑100 (MQ=255)
                                                                                          tatatTTTGTGCTTAGCGCCAACATCAATGCCGCGCGTAGGTTCATCCAGCAAAATGACCTTCATCTCTTCCGATAACCAGCGGCCCAGAATGGCttttt     <  2:1238944/100‑1 (MQ=255)
                                                                                            tatTTTGTGCTTAGCGCCAACATCAATGCCGCGCGTAGGTTCATCCAGCAAAATGACCTTCATCTCTTCCGATAACCAGCGGCCCAGAATGGCttttgct   <  1:2183010/100‑4 (MQ=255)
                                                                                            tatTTTGTGCTTAGCGCCAACATCAATGCCGCGCGTAGGTTCATCCAGCAAAATGACCTTCATCTCTTCCGATAACCAGCGGCCCAGAATGGCTTTTTg    >  1:2880731/1‑99 (MQ=255)
                                                                                             atTTTGTGCTTAGCGCCAACATCAATGCCGCGCGTAGTTTCATCCAGCAAAATGACCTTCATCTCGTCCGATAACCAGCGGCCCAGAATGGCTTTTTGCt  <  1:2473080/100‑1 (MQ=255)
                                                                                             atTTTGTGCTTAGCGCCAACATCAATGCCGCGCGTAGGTTCATCCAGCAAAATGACCTTCATCTCTTCCGATAACCAGCGGCCCAGAATGGCTTTTTGCt  >  1:1220603/1‑100 (MQ=255)
                                                                                                 |                                                                                               
ATCCGGTCGGCAACGCCGAGGACTTCAGGTAAGTCGCTGGAGGCAAACAGCACCGCCACGCCCTGCGCCGCCAGCGCATAAATTACGTTATATATTTCGTGCTTAGCGCCAACATCAATGCCGCGCGTAGGTTCATCCAGCAAAATGACCTTCATCTCTTCCGATAACCAGCGGCCCAGAATGGCTTTTTGCT  >  NC_000913/1983664‑1983856

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: