Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,161,946 A→G 100% A161A (GCA→GCG iceT → putative citrate/iron‑citrate/zinc‑citrate efflux transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,161,9460AG98.1% 333.3 / ‑2.9 102A161A (GCA→GCGiceTputative citrate/iron‑citrate/zinc‑citrate efflux transporter
Reads supporting (aligned to +/- strand):  ref base A (2/0);  new base G (46/54);  total (48/54)
Fisher's exact test for biased strand distribution p-value = 2.19e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.92e-01

Only 100 of 103 total aligned reads displayed.

CGCGAGCAATATATGGCGGCGATGACCTTTGTCACGTTACCCGGTCAGGTCGGTCCGCTGCTCGGTCCGGCGCTCGGCGGTCTGCTGGTGGAGTACGCATCGTGGCACTGGATCTTTTTGATCAACATTCCGGTGGGGATTATCGGTGCGATCGCCACATTGCTGTTAATGCCGAACTACACCATGCAGACGCG  >  NC_000913/2161848‑2162041
                                                                                                  |                                                                                               
cgcgAGCAATATATGGCGGCGATGACCTTGTCACGTTACCCGGTCAGGTCGGTCCGCTGCTCGGTCCGGCGCTCGGCGGTCTGCTGGTGGAGTACGCGTc                                                                                               <  1:1887196/100‑1 (MQ=255)
 gcgAGCAATATATGGCGGCGATGACCTTTGTCACTTTACCCGGTCAGGTCTGTCCGCTGCTCGGTCCGGCGCTCGGCGGTCTGCTGGTGGAGTACGCGTc                                                                                               <  1:2543023/100‑1 (MQ=255)
     gCAATATATGGCGGCGATGACCTTTGTCACGTTACCCGGTCAGGTCGGTCCGCTGCTCGGTCCGGCGCTCGGCGGTCTGCTGGTGGAGTACGCGTCGTgg                                                                                           >  2:44556/1‑100 (MQ=255)
      cAATATATGGCGGCGATGACCTTTGTCACGTTACCCGGTCAGGTCGGTCCGCTGCTCGGTCCGGCGCTCGGCGGTCTGCTGGTGGAGTACGCGTCGTGGc                                                                                          <  1:2021197/100‑1 (MQ=255)
      cAATATATGGCGGCGATGACCTTTGTCACGTTAACCGGTCAGGTCGGTCCGCTGCTCGGTCCGGCGCTCGACGGTCTGCTGGTGGAGTACGCGTCGTgg                                                                                           >  2:1824721/1‑99 (MQ=255)
        atatatGGCGGCGATGACCTTTGTCACGTTACCCGGTCAGGTCGGTCCGCTGCTCGGTCCGGCGTTCGGCGGTCTGCTTGTGGTGTACGCGTCGTGGCAc                                                                                        <  2:1453420/100‑1 (MQ=255)
        atatatGGCGGCGATGACCTTTGTCACGTTACCCGGTCAGGTCGGTCCGCTGCTCGGTCCGGCGCTCGGCGGTCTGCTGGTGGAGTACGCGTCGTgg                                                                                           <  1:705455/97‑1 (MQ=255)
        atatatGGCGGCGATGACCTTTGTCACGTTACCCGGTCAGGTCGGTCCGCTGCTCGGTCCGGCGCTCGGCGGTCTGCTGGTGGAGTACGCGTCGTGGCAc                                                                                        >  2:2718214/1‑100 (MQ=255)
         tatctGGCGGCGGTGACCTTTTTCACGATTCCCGGTCGTGTCGGTCGGCTGCTCGGTCCGGCGCTCGGCGGTCTGCTTGTGGAGTACGCGTCGTGGCACt                                                                                       <  2:1668643/100‑1 (MQ=255)
         tatatGGCGGCGATGACCTTTGTCACGTTACCCGGTCAGGTAGGTCCGCTGCTCGGTACGGCGCTTGGCGGTCTGCTGGTGGAGTACGCGTCGTGGCAc                                                                                        >  1:2987231/1‑99 (MQ=255)
          atatGGCGGCGATGACCTTTGTCACGTTACCCGGTCAGGTCGGTCCGCTGCTCGGTCCGGCGCTCGGCGGTCTGCTGGTGGAGTACGCGTCGTGGCACTg                                                                                      <  2:2810573/100‑1 (MQ=255)
            atGGCGGCGATGACCTTTGTCACGTTACCCGGTCAGGTCGGTCCGCTGCTCGGTCCGGCGCTCGGCGGTCTGCTGGTGGAGTACGCGTCGTGGCACTGGa                                                                                    >  2:643514/1‑100 (MQ=255)
            atGGCGGCGATGACCTTTGTCACGTTACCCGGTCAGGTCGGTCCGCTGCTCGGTCCGGCGCTCGGCGGTCTGCTGGTGGAGTACGCGTCGTGGCACTGGa                                                                                    >  2:1332758/1‑100 (MQ=255)
             tGGCGGCGATGACCTTTGTCACGTTACCCGGTCAGGTCGGTCCGCTGCTCGGTCCGGCGCTCGGCGGTCTGCTGGTGGAGTACGCGTCGTGGCACTGGa                                                                                    <  2:279195/99‑1 (MQ=255)
             tGGCGGCGATGACCTTTGTCACGTTACCCGGTCAGGTCGGTCCGCTGCTCGGTCCGGCGCTCGGCGGTCTGCTGGTGGAGTACGCGTCGTGGCACTGGAt                                                                                   <  1:1054865/100‑1 (MQ=255)
              ggcggcGATGACCTTTGTCACGTTACCCGGTCAGGTCGGTCCGCTGCTCGGTCCGGCGCTCGGCGGTCTGCTGGTGGAGTACGCGTCGTGGCACTGGATc                                                                                  >  1:1590134/1‑100 (MQ=255)
              ggcggcGATGACCTTTGTCACGTTACCCGGTCAGGTCGGTCCGCTGCTCGGTCCGGCGCTCGGCGGTCTGCTGGTGGAGTACGCGTCGTGGCACTGGATc                                                                                  >  1:2187410/1‑100 (MQ=255)
              ggcggcGATGACCTTTGTCACGTTACCCGGTCAGGTCGGTCCGCTGCTCGGTCCGGCGCTCGGCGGTCTGCTGGTGGAGTACGCGTCGTGGCACTGGATc                                                                                  >  2:2228858/1‑100 (MQ=255)
              ggcggcGATGACCTTTGTCACGTTACCCGGTCAGGTCGGTCCGCTGCTCGGTCCGGCGCTCGGCGGTCTGCTGGTGGAGTACGCGTCGTGGCACTGGATc                                                                                  >  2:1754811/1‑100 (MQ=255)
               gcggcgATGACCTTTGTCACGTTACCCGGTCAGGTCGGTCCGCTGCTCGGTCCGGCGCTCGGCGGTCTGCTGGTGGAGTACGCGTCGTGGCACTGGATc                                                                                  >  1:2615204/1‑99 (MQ=255)
               gcggcgATGACCTTTGTCACGTTACCCGGTCAGGTCGGTCCGCTGCTCGGTCCGGCGCTCGGCGGTCTGCTGGTGGAGTACGCGTCGTGGCACTGGATCt                                                                                 >  1:2413938/1‑100 (MQ=255)
                 tgcgATGACCTTTGTCACGTTACCCGGTCAGGTCGGTCCGCTGCTCGGTCCGGCGCTCGGCGGTCTGCTGGTGGAGTACGCGTCGTGGCACTGGATCttt                                                                               >  2:1195453/2‑100 (MQ=255)
                 ggcgATGACCTTTGTCACGTTACCCGGTCAGGTCGGTCCGCTGCTCGGTCCGGCGCTCGGCGGTCTGCTGGTGGAGTACGCGTCGTGGCACTGGATCttt                                                                               <  2:1007405/100‑1 (MQ=255)
                  gcgATGACCTTTGTCACGTTACCCGGTCAGGTCGGTCCGCTGCTCGGTCCGGCGCTCGGCGGTCTGCTGGTGGAGTACGCGTCGTGGCACTGGATCtttt                                                                              <  1:1908629/100‑1 (MQ=255)
                     aTGTCCCTTGTCACGTTACCCGGTCAGGTCGGTCCGCTGCTCGGTCCGGCGCTCGGCGGTCTGTTGGTGGAGTACGCGTCGTGGCACTGGATCTTTTTg                                                                            <  2:2615172/99‑1 (MQ=255)
                      tgtccTTTGTCAGGTGAGCCGGTCAGGTCGGTCCGCTGCTCGGTCCGGCGCTTGGCGGTCTGCTGGTGGAGTACGCGTCGTGGCACTGGATCTTTTTGa                                                                           <  2:2821339/96‑1 (MQ=255)
                      tGACCTTTGTCACGTTACCCGGTCAGGTCGGTCCGCTGCTCGGTCCGGCGCTCGGCGGTCTGCTGGTGGAGTACGCGTCGTGGCACTGGATCTTTTTGAt                                                                          >  2:522945/1‑100 (MQ=255)
                      tGACCTTTGTCACGTTACCCGGTCAGGTCGGTCCGCTGCTCGGTCCGGCGCTCGGCGGTCTGCTGGTGGAGTACGCGTCGTGGCACTGGATCTTTTTGAt                                                                          >  1:2514207/1‑100 (MQ=255)
                        aCCTTTGTCACGTTACCCGGTCAGGTCGGTCCGCTGCTCGGTCCGGCGCTCGGCGGTCTGCTGGTGGAGTACGCGTCGTGGCACTGGATCTTTTTGATc                                                                         <  2:2514178/99‑1 (MQ=255)
                         ccTTTGTCACGTTACCCGGTCAGGTCGGTCCGCTGCTCGGTCCGGCGCTCGGCGGTCTGCTGGTGGAGTACGCGTCGTGGCACTGGATCTTTTTGATCaa                                                                       >  2:2479904/1‑100 (MQ=255)
                         ccTTTGTCACGTTACCCGGTCAGGTCGGTCCGCTGCTCGGTCCGGCGCTCGGCGGTCTGCTGGTGGAGTACGCGTCGTGGCACTGGATCTTTTTGATCaa                                                                       >  2:1103657/1‑100 (MQ=255)
                         ccTTTGTCACGTTACCCGGTCAGGTCGGTCCGCTGCTCGGTCCGGCGCTCGGCGGTCTGCTGGTGGAGTACGCGTCGTGGCACTGGATCTTTTTGATCaa                                                                       >  1:690051/1‑100 (MQ=255)
                         ccTTTGTCACGTTACCCGGTCAGGTCGGTCCGCTGCTCGGTCCGGCGCTCGGCGGTCTGCTGGTGGAGTACGCGTCGTGGCACTGGATCTTTTTGATCaa                                                                       <  1:856576/100‑1 (MQ=255)
                         ccTTTGTCACGTTACCCGGTCAGGTCGGTCCGCTGCTCGGTCCGGCGCTCGGCGGTCTGCTGGTGGAGTACGCGTCGTGGCACTGGATCTTTTTGATCaa                                                                       <  1:1535438/100‑1 (MQ=255)
                         ccTTTGTCACGTTACCCGGTCAGGTCGGTCCGCTGCTCGGTCCGGCGCTCGGCGGTCTGCTGGTGGAGTACGCGTCGTGGCACTGGATCTTTTTGATCaa                                                                       <  2:2030706/100‑1 (MQ=255)
                         ccTTTGTCACGTTACCCGGACAGGTCGGTCCGCTGCTCGGTCCGGCGCTCGGCGGTCTGCTGGTGGTGTACGCGTCGTGGCACTGGATCTTTTTGATCa                                                                        >  2:1015656/1‑99 (MQ=255)
                           tttGTCACGTTACCCGGTCAGGTCGGTCCGCTGCTCGGTTCGGCGCTCGGCGGTCTGCTGGTGGAGTACGCGTCGTGGCACTGGATCTTTTTGATCAACa                                                                     <  1:848590/100‑1 (MQ=255)
                           tttGTCACGTTACCCGGTCAGGTCGGTCCGCTGCTCGGTCCGGCGCTCGGCGGTCTGCTGGTGGAGTACGCGTCGTGGCACTGGATCTTTTTGATCAACa                                                                     <  2:2360038/100‑1 (MQ=255)
                               tCACGTTACCCGGTCAGGTCGGTCCGCTGCTCGGTCCGGCGCTCGGCGGTCTGCTGGTGGAGTACGCGTCGTGGCACTGGATCTTTTTGATCAACATTcc                                                                 >  1:3022067/1‑100 (MQ=255)
                                cACGTTACCCGGTCAGGTCGGTCCGCTGCTCGGTCCGGCGCTCGGCGGTCTGCTGGTGGAGTACGCGTCGTGGCACTGGATCTTTTTGATCAACATTcc                                                                 <  2:1132810/99‑1 (MQ=255)
                                cACGTTACCCGGTCAGGTCGGTCCGCTGCTCGGTCCGGCGCTCGGCGGTCTGCTGGTGGAGTACGCATCGTGGCACTGGATCTTTTTGATCAACATTCCg                                                                >  2:474965/1‑100 (MQ=255)
                                cACGTTACCCGGTCAGGTCGGTCCGCTGCTCGGTCCGGCGCTCGGCGGTCTGCTGGTGGAGTACACGTCGTGGCACTGGATCTTTTTGATCAACATTCCg                                                                >  1:707243/1‑100 (MQ=255)
                                 aCGTTACCCGGTCAGGTCGGTCCGCTGCTCGGTTCGGCGTTCGGCGGTCTGCTGGTGGAGTACGCGTCGTGGCACTGGATCTTTTTGATCAACATTCCgg                                                               <  1:301426/100‑1 (MQ=255)
                                  cGTTACCCGGTCAGGTCGGTCCGCTGCTCGGTCCGGCGCTCGGCGGTCTGCTGGTGGAGTACGCGTCGTGGCACTGGATCTTTTTGATCAACATTCCGGt                                                              >  1:2989835/1‑100 (MQ=255)
                                     tACCCGGTCAGGTTGGTCCGCTGCTCGGTCCGGCGCTCGGCGGTCTGCTGGTGGAGTACGCGTCGTGGCACTGGATCTTTTTGATCAACATTCCGGTggg                                                           <  1:2076407/100‑1 (MQ=255)
                                     tACCCGGTCAGGTCGGTCCGCTGCTCGGTCCGGCGCTCGGCGGTCTGCTGGTGGAGTACGCGTCGTGGCACTGGATCTTTTTGATCAACATTCCGGTggg                                                           <  1:283820/100‑1 (MQ=255)
                                      aCCCGGTCAGGTCGGTCCGCTGCTCGGTCCGGCGCTCGGCGGTCTGCTGGTGGAGTACGTGTCGTGGCACTGGATCTTTTTGATCAACATTCCGGTgggg                                                          >  2:1225411/1‑100 (MQ=255)
                                      aCCCGGTCAGGTCGGTCCGCTGCTCGGTCCGGCGCTCGGCGGTCTGCTGGTGGAGTACGCGTCGTGGCACTGGATCTTTTTGATCAACATTCCGGTgggg                                                          >  2:2267299/1‑100 (MQ=255)
                                       cccGGTCAGGTCGGTCCGCTGCTCGGTCCGGCGCTCGGCGGTCTGCTGGTGGAGTACGCGTCGTGGCTCTGGATCTTTTTGATCAACATTCCGGTGGGGa                                                         <  1:2888653/100‑1 (MQ=255)
                                         cGGTCAGGTCGGTCCGCTGCTCTGTCCGGCGCTCGGCGGTCTGCTGGTGTAGTACGCGTCGTGGCACTGGATCTTTTTGATCAACATGCCGGTgggg                                                          <  2:2413909/97‑1 (MQ=255)
                                         cGGTCAGGTCGGTCCGCTGCTCGGTCCGGCGCTCGGCGGTCTGCTGGTGGAGTACGCGTCTTGGCACTGGATCTTTTTGATCAACATTCCGGTGGGGAtt                                                       <  2:752239/100‑1 (MQ=255)
                                         cGGTCAGGTCGGTCCGCTGCTCGGTCCGGCGCTCGGCGGTCTGCTGGTGGAGTACGCGTCGTGGCACTGGATCTTTTTGATCAACATTCCGGTGGGGAtt                                                       >  1:2144137/1‑100 (MQ=255)
                                         cGGTCAGGTCGGTCCGCTGCTCGGTCCGGCGCTCGGCGGTCTGCTGGTGGAGTACGCGTCGTGGCACTGGATCTTTTTGATCAACATTCCGGTGGGGAt                                                        >  2:2601560/1‑99 (MQ=255)
                                            tCAGGTCGGTCCGCTGCTCGGTCCGGCGCTCGGCGGTCTGCTGGTGGAGTACGCGTCGTGGCACTGGATCTTTTTGATCAACATTCCGGTGGGGATTATc                                                    <  1:1904192/100‑1 (MQ=255)
                                              aGGTCGGTCCGCTGCTCGGTCCGGCGCTCGGCGGTCTGCTGGTGGAGTACGCGTCGTGGCACTGGATCTTTTTGATCAACATTCCGGTGGGGATTATCgg                                                  <  1:1195464/100‑1 (MQ=255)
                                               ggtcggtcCGCTGCTCGGTCCGGCGCTCGGCGGTCTGCTGGTGGAGTACGCGTCGTGGCACTGGATCTTTTTGATCAACATTCCGGTGGGGATTATCGGt                                                 >  1:2254116/1‑100 (MQ=255)
                                               ggtcggtcCGCTGCTCGGTCCGGCGCTCGGCGGTCTGCTGGTGGAGTACGCGTCGTGGCACTGGATCTTTTTGATCAACATTCCGGTGGGGATTATCGGt                                                 >  2:1141412/1‑100 (MQ=255)
                                               ggtcggtcCGCTGCTCGGTCCGGCGCTCGGCGGTCTGCTGGTGGAGTACGAGTAATGGCACTGGATCTTTTTGATAAAAATTCCGGAGGGGATTATCg                                                   >  1:2417762/1‑98 (MQ=255)
                                                gtcggtcCGCTGCTCGGTCCGGCGCTCGGCGGTCTGCTGGTGGAGTACGCGTCGTGGCACTGGATCTTTTTGATCAACATTCCGGTGGGGATTATCGGTg                                                <  2:2914786/100‑1 (MQ=255)
                                                gtcggtcCGCTGCTCGGTCCGGCGCTCGGCGGTCTGCTGGTGGAGTACGCGTCGTGGCACTGGATCTTTTTGATCAACATTCCGGTGGGGATTATCGGTg                                                <  1:2479933/100‑1 (MQ=255)
                                                 tcggtcCGCTGCTCGGTCCGGCGCTCGGCGGTCTGCTGGTGGAGTACGCGTCGTGGCACTGGATCTTTTTGATCAACATTCCGGTGGTGATTATCGGTGc                                               <  1:1994634/100‑1 (MQ=255)
                                                 tcggtcCGCTGCTCGGTCCGGCGCTCGGCGGTCTGCTGGTGGAGTACGCGTCGTGGCACTGGATCTTTTTGATCAACATTCCGGTGGGGATTATCGGTGc                                               <  1:2331450/100‑1 (MQ=255)
                                                 tcggtcCGCTGCTCGGTCCGGCGCTCGGCGGTCTGCTGGTGGAGTACGCGTCGTGGCACTGGATCTTTTTGATCAACATTCCGGTGGGGATTATCGGTGc                                               <  1:1812173/100‑1 (MQ=255)
                                                 tcggtcCGCTGCTCGGTCCGGCGCTCGGCGGTCTGCTGGTGGAGTACGCGTCGTGGCACTGGATCTTTTTGATCAACATTCCGGTGGGGATTATCGGTGc                                               <  1:2474834/100‑1 (MQ=255)
                                                  cggtcCGCTGCTCGGTCCGGCGATCGGCGGTCTGCTGGTGGAGTACGCGTCGTGGCACTGGATCTTTTTGATCAACATTCCGGTGGGGATTATCGGTg                                                >  2:21101/1‑98 (MQ=255)
                                                    gtcCGCTGCTCGGTCCGGCGCTCGGCGGTCTGCTGGTGGAGTACGCGTCGTGGCACTGGATCTTTTTGATCAACATTCCGGTGGGGATTATCGGTGCGa                                             >  2:229949/1‑99 (MQ=255)
                                                    gtcCGCTGCTCGGTCCGGCGCTCGGCGGTCTGCTGGTGGAGTACGCGTCGTGGCACTGGATCTTTTTGATCAACATTCCGGTGGGGATTATCGGTGCGAt                                            >  1:2738317/1‑100 (MQ=255)
                                                    gtcCGCTGCTCGGTCCGGCGCTCGGCGGTCTGCTGGTGGAGTACGCGTCGTGGCACTGGATCTTTTTGATCAACATTCCGGTGGGGATTATCGGTGCGAt                                            >  1:1430223/1‑100 (MQ=255)
                                                      ccGCTGCTCGGTCCGGCGCTCGGCGGTCTGCTGGTGGAGTACGCGTCGTGGCACTGGATCTTTTTGATCAACATTCCGGTGGGGATTATCGGTGCGATCg                                          <  1:1008783/100‑1 (MQ=255)
                                                      ccGCTGCTCGGTCCGGCGCTCGGCGGTCTGCTGGTGGAGTACGCGTCGTGGCACTGGATCTTTTTGATCAAAATTCCGGTGGGGATTATCGGAGCAATc                                           >  1:2374183/1‑99 (MQ=255)
                                                       cGCTGCTCGGTCCGGCGCTCGGCGGTCTGCTGGTGGAGTACGCGTCGTGGCACTGGATCTTTTTGATCAACATTCCGGTGGGGATTATCGGTGCGATCGc                                         >  1:160815/1‑100 (MQ=255)
                                                        gctgctCGGTCCGGCGCTCGGCGGTCTGCTGGTGGAGTACGCGTCGTGGCACTGGATCTTTTCGATCACCATTCCGGCGGGGATTATCGGTGCGATCGcc                                        >  2:2043438/1‑100 (MQ=255)
                                                              cGGTCCGGCGCTCGGCGGTCTGCTGGTGGAGTACGCGTCGGGGCACTGGATCTTTTTGATCAACATTCCGGTGGTGATTATCGGTGCGATCGCCACAt                                    <  2:2957244/98‑1 (MQ=255)
                                                                  ccGGCGCTCGGCGGTCTGCTGGTGGAGTACGCGTCGTGGTACTGGATCTTTTTGATCAACATTCCGGTGGGGATTATCGGTGCGATCGCCACATTGCTGt                              <  1:815975/100‑1 (MQ=255)
                                                                    ggCGCTCGGCGGTCTGCTGGTGGAGTACGCGTCGTGGCACTGGATCTTTTTGATCAACATTCCGGTGGGGATTATCGGTGCGATCGCCACATTGCTGTTa                            <  2:2376807/100‑1 (MQ=255)
                                                                    ggCGCTCGGCGGTCTGCTGGTGGAGTACGCGTCGTGGCACTGGATCTTTTTGATCAACATTCCGGTGGGGATTATCGGTGCGATCGCCACATTGCTGTTa                            >  2:2719057/1‑100 (MQ=255)
                                                                    ggCGCTCGGCGGTCTGCTGGTGGAGTACGCGTCGTGGCACTGGATCTTTTTGATCAACATTCCGGTGAGGATTATCGGTGCGATCGCCACATTGCTGTTa                            >  1:686175/1‑100 (MQ=255)
                                                                     gcgcTCGGCGGTCTGCTGGTGGAGTACGCGTCGTGGCACTGGATCTTTTTGATCAACATTCCGGTGGGGATTATCGGTGCGATCGCCACATTGCTGTTaa                           >  2:1561325/1‑100 (MQ=255)
                                                                       gcTCGGCGGTCTGCTGGTGGAGTACGCGTCGTGGCACTGGATCTTTTTGATCAACATTCCGGTGGGGATTATCGGTGCGATCGCCACATTGCTGTTAAt                          <  2:707184/99‑1 (MQ=255)
                                                                        cTCGGCGGTCTGCTGGTGGAGTACGCGTCGTGGCACTGGATCTTTTTGATCAACATTCCGGTGGGGATTATCGGTGCGATCGCCACATTGCTGTTAATGc                        >  1:816254/1‑100 (MQ=255)
                                                                        cTCGGCGGTCTGCTGGTGGAGTACGCGTCGTGGCACTGGATCTTTTTGATCAACATTCCGGTGGGGATTATCGGTGCGATCGCCACATTGCTGTTAATGc                        <  1:5000/100‑1 (MQ=255)
                                                                          cggcggTCTGCTGGTGGAGTACGCGTCGTGGCACTGGATCTTTTTGATCAACATTCCGGTGGGGATTATCGGTGCGATCGCCACATTGCTGTTAATGCCg                      <  1:2013913/100‑1 (MQ=255)
                                                                           ggcggTCTGCTGGTGGAGTACGCGTCGTGGCACTGGATCTTTTTGATCAACATTCCGGTGGGGATTATCGGTGCGATCGCCACATTGCTGTTAATGCCGa                     >  1:2911586/1‑100 (MQ=255)
                                                                           ggcggTCTGCTGGTGGAGTACGCGTCGTGGCACTGGATCTTTTTGATCAACATTCCGGTGGGGATTATCGGTGCGATCGCCACATTGCTGTTAATGCCGa                     <  1:2186201/100‑1 (MQ=255)
                                                                             cggTCTGCTGGTGGAGTACGCGTCGTGGCACTGGATCTTTTTGATCAACATTCCGGTGGGGATTATCGGTGCGATCGCCACATTGCTGTTAATGCCGAAc                   <  2:1590115/100‑1 (MQ=255)
                                                                             cggTCTGCTGGTGGAGTACGCGTCGTGGCACTGGATCTTTTTGATCAACATTCCGGTGGGGATTATCGGTGCGATCGCCACATTGCTGTTAATGCCGAAc                   <  2:218914/100‑1 (MQ=255)
                                                                             cggTCTGCTGGTGGAGTACGCGTCGTGGCACTGGATCTTTTTGATCAACATTCCGGTGGGGATTATCGGTGCGATCGCCACATTGCTGTTAATGCCGAAc                   <  1:1735472/100‑1 (MQ=255)
                                                                              ggTCTGCTGGTGGAGTACGCGTCGTGGCACTGGATCTTTTTGATCAACATTCCGGTGGGGATTATCGGTGCGATCGCCACATTGCTGTTAATGCCGAACt                  <  1:1588783/100‑1 (MQ=255)
                                                                                tCTGCTGGTTGAGTACGCGTTTTTTTTTTGGATCTTTTTGTTTTATATTCCGGTGGGGATTATCGGTGCGATCGCCACATTGCTGTTAATGCCGAACTac                <  1:913056/100‑1 (MQ=255)
                                                                                 ctgctgGTGGAGTACGCGTCGTGGTACTGGATCTTTTTGATCAACATTCCGGTGGGGATTATCGGTGCGATCGCCACATTGCTGTTAATGCCGAACTaca               <  1:425281/100‑1 (MQ=255)
                                                                                   gctgGTGGAGTACGCGTCGTGGCACTGGATCTTTTTGATCAACATTCCGGTGGGGATTATCGGTGCGATCGCCACATTGCTGTTAATGCCGAACTACAcc             <  1:726245/100‑1 (MQ=255)
                                                                                    ctgGTGGAGTACGCGTCGTGGCACTGGATCTTTTTGATCAACATTCAGGTGGGGATTATCGGTGCGATCGCCACATTGCTGTTAATGCCGAACTACACCa            >  2:1676698/1‑100 (MQ=255)
                                                                                      ggtggAGTACGCGTCGTGGCACTGGATCTTTTTGATCAACATTCCGGTGGGGATTATCGGTGCGATCGCCACATTGCTGTTAATACCGAACTACACCATg          >  1:94729/1‑100 (MQ=255)
                                                                                      ggtggAGTACGCATCGTGGCACTGGATCTTTTTGATCAACATTCCGGTGGGGATTATCATAGCGATCGCCACATTGCTGTTAATGCCGAACTACACCATg          >  1:1578240/1‑100 (MQ=255)
                                                                                      ggtgaAGTACGCGTCGTGGCACTGGATCTTTTTGATCAACATTCCGGTGGGGATTATCGGTGCGATCGCCACATTGCTGTTAATGCCGAACTACACCATg          >  2:2740697/1‑100 (MQ=255)
                                                                                       gtggAGTACGCGTCTTGGCACTGGATCTTTTTGATCAACATTCCGGTGGGGATTATCGGTGCGATCGCCACATTGCTGTTAATGCCGAACTACACCATGc         <  2:2821945/100‑1 (MQ=255)
                                                                                       gtggAGTACGCGTCGTGGCACTGGATCTTTTTGATCAACATTCCGGTGGGGATTATCGGTGCGATCGCCACATTGCTGTTAATGCCGAACTACACCATGc         <  1:2228881/100‑1 (MQ=255)
                                                                                           aGTACGCGTCGTGGCACTGGATCTTTTTGATCAACATTCCGGTGGGGATTATCGGTGCGATCGCCACATTGCTGTTAATGCCGAACTACACCATGCa        >  2:1634209/1‑97 (MQ=255)
                                                                                             tACGCGTCGTGGCACTGGATCTTTTTGATCAACATTCCGGTGGGGATTATCGGTGCGATCGCCACATTGCTGTTAATGCCGAACTACACCATGCAGAcgc   <  1:1667092/100‑1 (MQ=255)
                                                                                              aCGCGTCGTGGCACTGGATCTTTTTGATCAACATTCCGGTGGGGATTATCGGTGCGATCGCCACATTGCTGTTAATGCCGAACTACACCATGCAGAcgcg  <  1:1958157/100‑1 (MQ=255)
                                                                                                  |                                                                                               
CGCGAGCAATATATGGCGGCGATGACCTTTGTCACGTTACCCGGTCAGGTCGGTCCGCTGCTCGGTCCGGCGCTCGGCGGTCTGCTGGTGGAGTACGCATCGTGGCACTGGATCTTTTTGATCAACATTCCGGTGGGGATTATCGGTGCGATCGCCACATTGCTGTTAATGCCGAACTACACCATGCAGACGCG  >  NC_000913/2161848‑2162041

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: