Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,168,420 A→T 17.0% intergenic (‑114/‑292) yegR ← / → yegS uncharacterized protein/phosphatidylglycerol kinase, metal‑dependent

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,168,4200AT17.0% 318.1 / 52.1 134intergenic (‑114/‑292)yegR/yegSuncharacterized protein/phosphatidylglycerol kinase, metal‑dependent
Reads supporting (aligned to +/- strand):  ref base A (60/50);  new base T (10/13);  total (71/63)
Fisher's exact test for biased strand distribution p-value = 3.66e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.16e-01

Only 100 of 135 total aligned reads displayed.

TTAAATACCAACGACACTCTCTAGTGTTTAAATATAATAATGGCATGATTATTATAATTGAATAGGATTATAATAAATGTTCTGTACAACATTTCCTACATAAGTAGGAATTACGGACATTGAGGCCCTTCAGGGTAACTCCATGGGGGCTTTAATATATTATATTGAAGATGCCACTGTTTAGTTGAATATTAGGTA  >  NC_000913/2168321‑2168518
                                                                                                   |                                                                                                  
ttAAATACCAACGACACTCTCTAGTGTTTAAATATAATAATGGCATGATTATTATAATTGAATAGGATTATAATAAATGTTCTGTACAACATTTCCTACa                                                                                                    <  2:238618/100‑1 (MQ=255)
     tACCAACGACACTCTCTAGTGTTTAAATATAATAATGGCATGATTATTATAATTGAATAGGATTATAATAAATGTTCTGTACAACATTTCCTACATAAGt                                                                                               >  1:2357715/1‑100 (MQ=255)
       ccAACGACACTCTCTAGTGTTTAAATATAATAATGGCATGATTATTATAATTGAATAGGATTATAATAAATGTTCTGTACAACATTTCCTACATAAGTAg                                                                                             <  1:1198811/100‑1 (MQ=255)
        cAACGACACTCTCTAGTGTTTAAATATAATAATGGCATGATTATTATAATTGAATAGGATTATAATAAATGTTCTGTACAACATTTCCTACATAAGTAgg                                                                                            >  2:1457984/1‑100 (MQ=255)
        cAACGACACTCTCTAGTGTTTAAATATAATAATGGCATGATTATTATAATTGAATAGGATTATAATAAATGTACTGTACAACATTTCCTACATAAGTAgg                                                                                            >  2:609164/1‑100 (MQ=255)
          aCGACACTCTCTAGTGTTTAAATATAATAATGGCATGATTATTATAATTGAATAGGATTATAATAAATGTTCTGTACAACATTTCCTACATAAGTAGGaa                                                                                          >  2:898994/1‑100 (MQ=255)
           cGAGACTCTCTAGTGTTTAAATATAATAATGGCATGATTATTATAATTGAATAGGATTATAATAAATGTTCTGTACAACATTTCCTACATAAGTAgg                                                                                            <  2:2437886/97‑1 (MQ=255)
           cGACACTCTCTAGTGTTTAAATATAATAATGGCATGATTATTATAATTGAATAGGATTATAATAAATGTTCTGTACAACATTTCCTACATAAGTAGGAAt                                                                                         >  1:1788502/1‑100 (MQ=255)
            gACACTCTCTAGTGTTTAAATATAATAATGGCATGATTATTATAATTAAATAGGATTATAATAAATGTTCTGTACAACATTTCCTACTTATGTAGGaaat                                                                                        <  2:1114543/100‑3 (MQ=255)
             ncacTCTCTAGTGTTTAAATATAATAATGGCATGATTATTATAATTGAATAGGATTATAATAAATGTTCTGTACAACATTTCCTACATAAGTAGGAATTa                                                                                       >  2:673591/2‑100 (MQ=255)
             acacTCTCTAGTGTTTAAATATAATAATGGCATGATTATTATAATTGAATAGGATTATAATAAATGTTCTGTACAACATTTCCTACATAAGTAGGAATTa                                                                                       >  1:1672217/1‑100 (MQ=255)
              cacTCTCTAGTGTTTAAATATAATAATTGCATGATTATTATAATTGAATAGGATTATAATAAATGTTCTGTACAACATTTCCTACTTATGTAGGaaat                                                                                        <  1:1249024/98‑3 (MQ=255)
                ctctctAGTGTTTAAATATAATAATGGCATGATTATTATAATTGAATAGGATTATAATAAATGTTCTGTACAACATTTCCTACATAAGTAGGAATTACgg                                                                                    <  1:64827/100‑1 (MQ=255)
                ctctctAGTGTTTAAATATAATAATGGCATGATTATTATAATTGAATAGGATTATAATAAATGTTCTGTACAACATTTCCTACATAAGTAGGAATTACgg                                                                                    >  2:2497446/1‑100 (MQ=255)
                ctctctAGTGTGTAAATATAATAATGGCATGATTATTATAATTGAATAGGATTATAATAAATGTTCTGTACAACATTTCCTACTTATGTAGGaaat                                                                                        <  1:2994568/96‑3 (MQ=255)
                 tctctATTGTTTAAATATAATAATGGCATGATTATTATAATTGAATAGGATTATAATAAATGTTCTGTACAACATTTCCTACTTATGTAGGaaatgttg                                                                                    <  1:1292962/99‑7 (MQ=255)
                 tctctAGTGTTTAAATATAATAATGGCATGATTATTATAATTGAATAGGATTATAATAAATGTTCTGTACAACATTTCCTACTTATGTAGGaaatgttgt                                                                                   <  2:2286878/100‑8 (MQ=255)
                 tctctAGTGTTTAAATATAATAATGGCATGATTATTATAATTGAATAGGATTATAATAAATGTTCTGTACAACATTTCCTACATAAGTAGGAATTACGGa                                                                                   >  1:2398117/1‑100 (MQ=255)
                   tctTGTTTTTAAATATAATAATGGCATGCATATTATAATTGAATAGGATTATAATAAATGTTCTCTACAACATTTCCTACTTATGTAGGaaatgtt                                                                                     <  1:1089843/96‑6 (MQ=255)
                   tctTGTGTTTAAATATAATAATTTCATTATTATTATAATTGAATATGATTATAATAAATGTTCTGTACAACATTTCCTACATAAGTAGGAATTACgg                                                                                    <  1:1802211/97‑1 (MQ=255)
                   tctAGTTTTTAAATATAATAATGGCATGATTATTATAATTGAATAGGATTATAATAAATGTTCTGTACAACATTTCCTACATAAGTAGGAATTACGGAc                                                                                  <  2:2841770/99‑1 (MQ=255)
                   tctAGTGTTTAAATATAATAATGGCATGATTATTATAATTGAATAGGATTATAATAAATGTTCTGTACAACATTTCCTACATAAGTAGGAATTACGGACa                                                                                 <  2:2734136/100‑1 (MQ=255)
                     tAGTGTTTAAATATAATAATGGCATGATTATTATAATTGAATAGGATTATAATAAATGTTCTGTACAACATTTCCTACATAAGTAGGAATTACGGACAtt                                                                               >  2:1596400/1‑100 (MQ=255)
                         gtTTAAATATAATCATGGCATGATTATTATAATTGAATAGGATTATAATAAATGTTCTGTACAACATTTCCTACATAAGTAGGAATTACGGACATTGAgg                                                                           >  1:2040885/1‑100 (MQ=255)
                         gtTTAAATATAATAATGGCATGATTAATATAATTGAATAGGATTATAATAAATGTTCTGTACAACATTTCCTACATAAGTAGGAATTACGGACATTGAgg                                                                           >  1:1849792/1‑100 (MQ=255)
                          tttAAATATAATAATGGCATGATTATTATAATTGAATAGGATTATAATAAATGTTCTGTACAACATTTCCTACATAAGTAGGAATTACGGACATTGAGGc                                                                          >  1:1441203/1‑100 (MQ=255)
                           ttAAATATAATAATGGCATGATTATTATAATTGAATAGGATTATAATAAATGTTCTGTACAACATTTCCTACATAAGTAGGAATTACGGACATTGAgg                                                                           <  2:2334435/98‑1 (MQ=255)
                           ttAAATATAATAATGGCATGATTATTATAATTGAATAGGATTATAATAAATGTTCTGTACAACATTTCCTACATAAGTAGGAATTACGGACATTGAGGcc                                                                         >  1:607661/1‑100 (MQ=255)
                             aaaTATAATAATGGCATGATTATTATAATTGAATAGGATTATAATAAATGTTCTGTACAACATTTCCTACATAAGTAGGAATTACTTACATTGAGGCCCt                                                                       >  2:1901961/1‑100 (MQ=255)
                             aaaTATAATAATGGCATGATTATTATAATTGAATAGGATTATAATAAATGTTCTGTACAACATTTCCTACATAAGTAGGAATTACGGACATTGAGGCCCt                                                                       >  2:1253929/1‑100 (MQ=255)
                                  taataaTGGCATGATTATTATAATTGAATTGTATTTTTATAAATGTTCTGTACAACATTTCCTACATAAGTAGGAATTACGGACATTGAGGCCCTTCAgg                                                                  <  1:24811/100‑1 (MQ=255)
                                  taataaTGGCATGATTATTATAATTGAATAGGATTATAATAAATGTTCTGTACAACATTTCCTACATAAGTAGGAATTACGGACATTGAGGCCCTTCAgg                                                                  <  2:1518378/100‑1 (MQ=255)
                                     taatGGCATGATTATTATAATTGAATAGGATTATAATAAATGTTCTGTACAACATTTCCTACATAAGTAGGAATTACGGACATTGAGGCCCTTCAGGGTa                                                               >  2:2120720/1‑100 (MQ=255)
                                       atGGCATGATTATTATAATTGAATAGGATTATAATAAATGTTCTGTACAACATTTCCTACATAAGTAGGAATTACGGACATTGAGGCCCTTCAGGGTAAc                                                             >  1:2276008/1‑100 (MQ=255)
                                           cATGATTATTATAATTGAATAGGATTATAATAAATGTTCTGTACAACATTTCCTACATAATTAGGAATTACGGACATTCATGCCCTTCAgaagaac                                                             >  2:3039525/1‑90 (MQ=255)
                                           cATGATTATTATAATTGAATAGGATTATAATAAATGTTCTGTACAACATTTCCTACATAAGTAGGAATTACGGACATTGAGGCCCTTCAGGGTAACTCCa                                                         >  2:2975030/1‑100 (MQ=255)
                                             ttattattATAATTGAATATGATTATAATAAATGTTTTGTACAACATTTCCTACATCAGTTGGAATTACGGACATTGAGGCCCTTCAcgg                                                                 <  2:2026190/88‑1 (MQ=255)
                                             tGATTATTATAATTGAATAGGATTATAATAAATGTTCTGTACAACATTTCCTACATAAGTAGGAATTACGGACATTGAGGCCCTTCAGGGTAACTCCATg                                                       >  2:2419910/1‑100 (MQ=255)
                                              gATTATTATAATTGAATAGGATTATAATAAATGTTCTGTACAACATTTCCTACATAAGTAGGAATTACGGACATTGAGGCCCTTCAGGGTAACTCCATg                                                       >  1:2917821/1‑99 (MQ=255)
                                               attattATAATTGAATAGGATTATAATAAATGTTCTGTACAACATTTCCTACATAAGTAGGAATTACGGACATTGAGGCCCTTCAGGGTAACTCCATggg                                                     <  1:68481/100‑1 (MQ=255)
                                                   ttatAATTGAATAGGATTATAATAAATGTTCTGTACAACATTTCCTACATAAGTAGGAATTACGGACATTGAGGCCCTTCAGGGTAACTCCATGGGGGCt                                                 >  2:1748600/1‑100 (MQ=255)
                                                   ttatAATTGAATAGGATTATAATAAATGTTCTGTACAACATTTCCTACATAAGTAGGAATTACGGACATTAAGGCCCTTCAGGGTAACTCCATGGGGTCt                                                 >  2:246228/1‑100 (MQ=255)
                                                        aTTGAATAGGATTATAATAAATGTTCTGTACAACATTTCCTACTTATGTAGGAATTACGGACATTGAGGCCCTTCAGGGTAACTCCATGGGGGCTTTAat                                            <  2:2429347/100‑1 (MQ=255)
                                                        aTTGAATAGGATTATAATAAATGTTCTGTACAACATTTCCTACATAAGTAGGAATTACGGACATTGATGCCCTTCCGGGTACCTCCATGGGGGCTTTaa                                             >  2:1750637/1‑99 (MQ=255)
                                                        aTTGAATAGGATTATAATAAATGTTCTGTACAACATTTCCTACATAAGTAGGAATTACGGACATTGAGGCCCTTCAGGGTAACTCCATGGGGGCTTTAat                                            <  1:998496/100‑1 (MQ=255)
                                                        aTTGAATAGGATTATAATAAATGTTCTGTACAACATTTCCTACATAAGTAGGAATTACGGACATTGAGGCCCTTCAGGGTAACTCCATGGGGGCTTTAat                                            >  2:225842/1‑100 (MQ=255)
                                                            aaTAGGATTATAATAAATGTTCTGTACAACATTTCCTACATAAGTAGGAATTACGGACATTGAGGCCCTTCAGGGTAACTCCATGGGGGCTTTAatatat                                        >  2:877317/1‑100 (MQ=255)
                                                            aaTAGGATTATAATAAATGTTCTGTACAACATTTCCTACATAAGTAGGAATTACGGACATTGAGGCCCTTCAGGGTAACTCCATGGGGGCTTTAatatat                                        >  1:2518932/1‑100 (MQ=255)
                                                            aaTAGGATTATAATAAATGTTCTGTACAACATTTCCTACATAAGTAGGAATTACGGACATTGAGGCCCTTCAGGGTAACTCCATGGGGGCTTTAatatat                                        <  2:2986131/100‑1 (MQ=255)
                                                              tAGGATTATAATAAATGTTCTGTACAACATTTCCTACATAAGTAGGAATTACGGACATTGAGGCCCTTCAGGGTAACTCCATGGGGGCTTTAAtatatta                                      <  1:1993366/100‑1 (MQ=255)
                                                               aGGATTATAATAAATGTTCTGTACAACATTTCCTACATAAGTAGGAATTACGGACATTGAGGCCCTTCAGGGTAACTCCATGGGGGCTTTAAtatattat                                     <  1:2548519/100‑1 (MQ=255)
                                                                 gATTATAATAAATGTTCTGTACAACATTTCCTACATAAGTAGGAATTACGGACATTGAGGCCCTTCAGGGTAACTCCATGGGGGCTTTAAtatattatat                                   <  2:2368166/100‑1 (MQ=255)
                                                                  aTTATAATAAATGTTCTGTACAACATTTCCTACATAAGTAGGAATTACGGACATTGAGGCTCTTCAGGGTAACTCCATGGGGGCTTTAATatattatatt                                  <  1:61654/100‑1 (MQ=255)
                                                                  aTTATAATAAATGTTCTGTACAACATTTCCAACGTAAGTAGGAATTACGGACATTGAGGCCCTTCAGGGTAACTCCATGGGGGCTTTAAAatattatatt                                  >  2:1990641/1‑100 (MQ=255)
                                                                     ataataAATGTTCTGTACAACATTTCCTACATAAGTAGGAATTACGGACATTGAGGCCCTTCAGGGTAACTCCATGGGGGCTTTAATATATTATATTGaa                               >  2:2015819/1‑100 (MQ=255)
                                                                      taataaATGTTCTGTACAACATTTCCTACATAAGTAGGAATTACGGACATTGAGGCCCTTCAGGGTAACTCCATGGGGGCTTTAATATATTATATTGAAg                              <  2:190417/100‑1 (MQ=255)
                                                                      taataaATGTTCTGTACAACATTTCCTACATAAGTAGGAATTACGGACATTGAGGCCCTTCAGGGTAACTCCATGGGGGCTTTAATATATTATATTGAAg                              <  1:2712300/100‑1 (MQ=255)
                                                                      taataaATGTTCTGTACAACATTTCCTACATAAGTAGGAATTACGGACATTGAGGCCCTTCAGGGTAACTCCATGGGGGCTTTAATATATTATATTGAAg                              >  2:242572/1‑100 (MQ=255)
                                                                       aataaATGTTCTGTACAACATTTCCTACATAAGTAGGAATTACGGACATTGAGGCCCTTCAGGGTAACTCCATGGGGGCTTTAATATATTATATTGaa                               >  1:1124532/1‑98 (MQ=255)
                                                                       aataaATGTTCTGTACAACATTTCCTACATAAGTAGGAATTACGGACATTGAGGCCCTTCAGGGTAACTCCATGGGGGCTTTAATATATTATATTGAAGa                             >  2:2093142/1‑100 (MQ=255)
                                                                        ataaATGTTCTGTACAACATTTCCTACATAAGTAGGAATTACGGACATTGAGGCCCTTCAGGGTAACTCCATGGGGGCTTTAATATATTATATTGAAGAt                            >  2:914311/1‑100 (MQ=255)
                                                                        ataaATGTTCTGTACAACATTTCCTACATAAGTAGGAATTACGGACATTGAGGCCCTTCAGGGTAACTCCATGGGGGCTTTAATATATTATATTGAAGAt                            >  2:2385360/1‑100 (MQ=255)
                                                                        ataaATGTTCTGTACAACATTTCCTACATAAGTAGGAATTACGGACATTGAGGCCCTTCAGGGTAACTCCATGGGGGCTTTAATATATTATATTGAAGAt                            >  2:1117941/1‑100 (MQ=255)
                                                                        ataaATGTTCTGTACAACATTTCCTACATAAGTAGGAATTACGGACATTGAGGCCCTTCAGGGTAACGCCATGGTGGCTTTAATATATTATATTGa                                <  2:2977996/96‑1 (MQ=255)
                                                                          aaaTGTTCTGTACAACATTTCCTACATAAGTAGGAATTACGGACATTGAGGCCCTTCAGGGTAACTCCATGGGGGCTTTAATATATTATATTGAAGATGc                          <  1:877323/100‑1 (MQ=255)
                                                                           aaTGTTCTGTACAACATTTCCTACATAAGTAGGAATTACGGACATTGAGGCCCTTCAGGGTAACTCCATGGGGGCTTTAATATATTATATTGAAGATGcc                         <  1:1703069/100‑1 (MQ=255)
                                                                           aaTGTTCTGTACAACATTTCCTACATAAGTAGGAATTACGGACATTGAGGCCCTTCAGGGTAACTCCATGGGGGCTTTAATATATTATATTGAAGATGcc                         <  2:2182292/100‑1 (MQ=255)
                                                                              gTTCTGTGCAACATTTCCTACATAAGTAGGAATTACGGACATTGAGGCCCTTCAGGGTAACTCCATGGGGGCTTTAATATATTATATTGAAGATGCCACt                      >  2:1095051/1‑100 (MQ=255)
                                                                              gTTCTGTACAACATTTCCTACATAAGTAGGAATTACGGACATTGAGGCCCTTCAGGGTAACTCCATGGGGGCTTTAATATATTATATTGAAGATGCCACt                      >  1:2459517/1‑100 (MQ=255)
                                                                                 cTGTACAACATTTCCTACATAAGTAGGAATTACGGACATTGAGGCCCTTCAGGGTAACTCCATGGGGGCTTTAATATATTATATTGAAGATGCCACTGtt                   <  1:2725503/100‑1 (MQ=255)
                                                                                  tGTACAACATTTCCTACATAAGTAGGAATTACGGACATTGAGGCCCTTCAGGGTAACTCCATGGGGGCTTTAATATATTATATTGAAGATGCCACTGttt                  <  2:2528112/100‑1 (MQ=255)
                                                                                   tgtccgtaattCCTACTTATGTAGGAATTACGGACATTGAGGCCCTTCAGGGTAACTCCATGGGGGCTTTAATATATTATATTGAAGATGCCACTGTTTa                 >  1:955484/10‑100 (MQ=38)
                                                                                   gTACAACATTTCCTACATAAGTAGGAATTACGGACATTGAGGCCCTTCAGGGTAACTCCATGGGGGCTTTAATATATTATATTGAAGATGCCACTGTTTa                 >  1:848146/1‑100 (MQ=255)
                                                                                    tACAACATTTCCTACATAAGTAGGAATTACGGACATTGAGGCCCTTCAGGGTAACTCCATGGGGGCTTTAATATATTATATTGAAGATGCCACTGTTTAg                <  1:985544/100‑1 (MQ=255)
                                                                                      ccgtaattCCTACTTATGTAGGAATTACGGACATTGAGGCCCTTCAGGGTAACTCCATGGGGGCTTTAATATATTATATTGAAGATGCCACTGTTTAGtt              >  1:2897999/7‑100 (MQ=255)
                                                                                      ccgtaattCCTACTTATGTAGGAATTACGGACATTGAGGCCCTTCAGGGTAACTCCATGGGGGCTTTAATATATTATATTGAAGATGCCACTGTTTAGtt              >  2:1566921/7‑100 (MQ=255)
                                                                                      caacaTTTCCTGCTTAAGTGGAAATTAGGGACATTTTGGCGCTTCAGGGTAACTCCATGGGGGCTTTATTATATTATATTGAAGATGCCACTGTTTAGtt              <  2:827325/100‑1 (MQ=255)
                                                                                      caacaTTTCCTACATAAGTAGGAATTACGGACATTGAGACCCTTCAGGGTAACTCCATGGGGGCTTTAATATATTATATTGAAGATGCCACTGTTTAGtt              >  2:1863563/1‑100 (MQ=255)
                                                                                       cgtaattCCTACTTATGTAGGAATTACGGACATTGAGGCCCTTCAGGGTAACTCCATGGGGGCTTTAATATATTATATTGAAGATGCCACTGTTTAGTTg             <  2:955478/95‑1 (MQ=255)
                                                                                       cgtaattCCTACTTATGTAGGAATTACGGACATTGAGGCCCTTCAGGGTAACTCCATGGGGGCTTTAATATATTATATTGAAGATGCCACTGTTTAGTTg             >  1:1863442/6‑100 (MQ=255)
                                                                                       aacaTTTCCTACATAAGTATGAATTACGGACATTTAGTCCCTTCATGGTAACTCCATGGGGGATTTAATATATTATATTAAAGATGCCACTGTTTAGTTg             <  1:1351898/100‑1 (MQ=255)
                                                                                       aacaTTTCCTACATAAGTAGGAATTACGGACATTGAGGCCCTTCAGGGTAACTCCATGGGGGCTTTAATATATTATATTGAAGATGCCACTGTTTAGTTg             >  2:1911879/1‑100 (MQ=255)
                                                                                       aacaTTTCCTACATAAGTAGGAATTACGGACATTGAGGCCCTTCAGGGTAACTCCATGGGGGCTTTAATATATTATATTGAAGATGCCACTGTTTAGTTg             <  1:847866/100‑1 (MQ=255)
                                                                                       aacaTTTCCTACATAAGTAGGAATTACGGACATTGAGGCCCTTCAGGGTAACTCCATGGGGGCTTTAATATATTATATTGAAGATGCCACTGTTTAGTTg             <  2:667966/100‑1 (MQ=255)
                                                                                        gtaattCCTACTTATGTAGGAATTACGGACATTGAGGCCCTTCAGGGTAACTCCATGGGGGCTTTAATATATTATATTGAAGATGCCACTGTTTAGTTGa            >  1:1022500/5‑100 (MQ=255)
                                                                                        gtaattCCTACTTATGTAGGAATTACGGACATTGAGGCCCTTCAGGGTAACTCCATAGGGGCTTTAATATATTATATTGAAGATGCCACTGTTTAGTTGa            >  1:1661324/5‑100 (MQ=255)
                                                                                        acaTTTCCTACATAAGTAGGAATTACGGACATTGAGGCCCTTCAGGGTAACTCCATGGGGGCTTTAATATATTATATTGAAGATGCCACTGTTTAGTTGa            >  1:115751/1‑100 (MQ=255)
                                                                                         caTTTCCTACATAAGTAGGAATTACGGACATTGAGGCCCTTCAGGGTAACTCCATGGGGGCTTTAATATATTATATTGAAGATGCCACTGTTTAGTTGaa           >  1:1774995/1‑100 (MQ=255)
                                                                                          aattCCTACTTATGTAGGAATTACGGACATTGAGGCCCTTCAGGGTAACTCCATGGGGGCTTTAATATATTATATTGAAGATGCCACTGTTTAGTTGa            >  2:2796865/3‑98 (MQ=255)
                                                                                          aTTTCCTACATAAGTAGGAATTACGGACATTGAGGCCCTTCAGGGTAACTCCATGGGTGTTTTAATATATTATATTGAAGATGCCACTGTTTAGTTGa            <  2:1872267/98‑1 (MQ=255)
                                                                                          aTTTCCTACATAAGTAGGAATTACGGACATTGAGGCCCTTCAGGGTAACTCCATGGGGGCTTTAATATATTATATTGAAGATGCCACTGTTTAGTTGAat          >  2:2403032/1‑100 (MQ=255)
                                                                                          aTTTCCTACATAAGTAGGAATTACGGACATTGAGGCCCTTCAGGGTAACTCCATGGGGGCTTTAATATATTATATTGAAGATGCCACTGTTTAGTTGAat          <  1:166549/100‑1 (MQ=255)
                                                                                          aTTTCCTACATAAGTAGGAATTACGGACATTGAGGCCCTTCAGGGTAACTCCATGGGGGCTTTAATATATTATATTGAAAATGCCACTGTTTAGTTGAat          <  2:1849771/100‑1 (MQ=255)
                                                                                           tttCCTACATAAGTAGGAATTACGGACATTGAGGCCCTTCAGGGTAACTCCATGGGGGCTTTAATATATTATATTGAAGATGCCACTGTTTAGTTGAata         >  2:318511/1‑100 (MQ=255)
                                                                                              ccTACTTATGTAGGAATTACGGACATTGAGGCCCTTCAGGGTAACTCCATGGGGGCTTTAATATATTATATTGAAGATGCCACTGTTTAGTTGAATATTa      >  2:2169523/1‑100 (MQ=255)
                                                                                              ccTACTTATGTAGGAATTACGGACATTGAGGCCCTTCAGGGTAACTCCATGGGGGCTTTAATATATTATATTGAAGATGCCACTGTTTAGTTGAATATTa      >  2:1702718/1‑100 (MQ=255)
                                                                                              ccTACATAAGTAGGAATTACGGACATTGAGGCCCTTCAGGGTAACTCCATGGTGGCTTTAATATATTATATTGAAGATGCCACTGTTTAGTTGAATATTa      <  2:2245547/100‑1 (MQ=255)
                                                                                                tACATAAGTAGGAATTACGGACATTGAGGCCCTTCAGGGTAACTCCATGGGGGCTTTAATATATTATATTGAAGATGCCACTGTTTAGTTGAATATTAgg    <  2:2347913/100‑1 (MQ=255)
                                                                                                tACATAAGTAGGAATTACGGACATTGAGGCCCTTCAGGGTAACTCCATGGGGGCTTTAATATATTATATTGAAGATGCCACTGTTTAGTTGAATATTAgg    <  1:912558/100‑1 (MQ=255)
                                                                                                 aCATAAGTAGGAATTACGGACATTGAGGCCCTTCAGGGTAACTCCATGGGGGCTTTAATATATTATATTGAAGATGCCACTGTTTAGTTGAATATTAGGt   <  2:328253/100‑1 (MQ=255)
                                                                                                   |                                                                                                  
TTAAATACCAACGACACTCTCTAGTGTTTAAATATAATAATGGCATGATTATTATAATTGAATAGGATTATAATAAATGTTCTGTACAACATTTCCTACATAAGTAGGAATTACGGACATTGAGGCCCTTCAGGGTAACTCCATGGGGGCTTTAATATATTATATTGAAGATGCCACTGTTTAGTTGAATATTAGGTA  >  NC_000913/2168321‑2168518

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: