| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 2,197,365 | G→A | 100% | pseudogene (892/2847 nt) | yehH → | DUF4132 family pseudogene; defective yehI paralog; molybdate metabolism regulator, first fragment; molybdate metabolism regulator, second fragment 2; yehI' molR mutations have phenotypes and the N‑terminal molR fragment of yehI' may be expressed and may have retained or evolved MolR function |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,197,365 | 0 | G | A | 97.8% | 258.7 / ‑3.8 | 85 | pseudogene (892/2847 nt) | yehH | DUF4132 family pseudogene; defective yehI paralog; molybdate metabolism regulator, first fragment; molybdate metabolism regulator, second fragment 2; yehI' molR mutations have phenotypes and the N‑terminal molR fragment of yehI' may be expressed and may have retained or evolved MolR function |
| Reads supporting (aligned to +/- strand): ref base G (1/1); new base A (47/36); total (48/37) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.64e-01 | |||||||||||
TACTTGGAGCAATATTCCAGATTTGAATGACGCCTACGTAAACATTTATCACTGACGGAAGAGTCTCTCTGGCAAAAATGTGCACAAAAACTTATTGCCGCAATTCCACATATTCCAGAATGGCGGCAACCATTAATTGCTTTGTTATTACCCGAAAAACCAGAAATTGCACATGAAATTGCCCAGCGTCTAC > NC_000913/2197269‑2197461 | tACTTGGAGCAATATTCCAGATTTGAATGACGCCTACGTAAACATTTATCACTGACGGAAGAGTCTCTCTGGCAAAAATGTGCACAAAAACTTATTACCg > 1:2082358/1‑100 (MQ=255) aCTTGGAGCAATATTCCAGATTTGAATGACGCCTACGTAAACATTTATCACTGACGGAAGAGTCTCTCTGGCAGAAATGTGCACAAAAACTTATTACCGc > 2:2553702/1‑100 (MQ=255) aCTTGGAGCAATATTCCAGATTTGAATGACGCCTACGTAAACATTTATCACTGACGGAAGAGTCTCTCTGGCAAAAATGTGCACAAAAACTTATTACCGc > 1:563930/1‑100 (MQ=255) aCTTGGAGCAATATTCCAGATTTGAATGACGCCTACGTAAACATTTATCACTGACGGAAGAGTCTCTCTGGCAAAAATGTGCACAAAAACTTATTACCGc > 1:554566/1‑100 (MQ=255) gAGCAATATTCCAGATTTGAATGACGCCTACGTAAACATTTATCACTGACGGAAGAGTCTCTCTGGCAAAAATGTGCACAAAAACTTATTACCGAAATTc > 1:1069376/1‑100 (MQ=255) aGCAATATTCCAGAGTTGAATGACGCCTACCTAAACATTTATCACTGACGGAAGAGTCTCTCTGGCAAAAATGTAAAAAAAAACTTATTACCGCAAtt < 2:2420588/98‑1 (MQ=255) ccatatTCCAGATTTAAATAACGACTACGTAAACATTTATCACTGACGGAAAAGTATCTCTGGCAAATATGTGCACAAAAAATTATTACCGCAATTCca > 2:29281/3‑99 (MQ=255) cAATATTCCAGATTTGACGGACGCCTACGTAAACATTTATTACTGACGGAATAGTCTCTCTGGCAAAAATGTGCACAAAATCTTATTACCGCAATTCcan < 2:2229289/100‑2 (MQ=255) aaTATTCCAGATTTGAATGACGCCTACGTAAACATTTATCACTGACGGAAGAGTCTCTCTGGCAAAAATGTGCACAAAAACTTATTACCGCAATTCcaca > 2:179730/1‑100 (MQ=255) aaTATTCCAGATTTGAATGACGCCTACGTAAACATTTATCACTGACGGAAGAGTCTCTCTGGCAAAAATGTGCACAAAAACTTATTACCGCAATTCcaca < 1:880411/100‑1 (MQ=255) aaTATTCCAGATTTGAATGACGCCTACGTAAACATTTATCACTGACGGAAGAGTCTCTCTGGCAAAAATGTGCACAAAAACTTATTACCGCAATTCcaca > 1:498532/1‑100 (MQ=255) atTCCAGATTTGAATGACGCCTACGTAAACATTTATCACTGACGGAAGAGTCTCTCTGGCAAAAATGTGCACAAAAACTTATTACCGCAATTCCACatat > 2:425323/1‑100 (MQ=255) atTCCAGATTTGAATGACGCCTACGTAAACATTTATCACTGACGGAAGAGTCTCTCTGGCAAAAATGTGCACAAAAACTTATTACCGCAATTCCACatat > 1:1270160/1‑100 (MQ=255) ttCCAGATTTGAATGACGCCTACGTAAACATTTATCACTGACGGAAGAGTCTCTCTGGCAAAAATGTGCACAAAAACTTATTACCGCAATTCCACATAtt > 2:455982/1‑100 (MQ=255) ttCCAGATTTGAATGACGCCTACGTAAACATTTATCACTGACGGAAGAGTCTCTCTGGCAAAAATGTGCACAAAAACTTATTACCGCAATTCCACATAtt > 1:2350638/1‑100 (MQ=255) ttCCAGATTTGAATGACGCCTACGTAAACATTTATCACTGACGGAAGAGTCTCTCTGGCAAAAATGTGCACAAAAACTTATTACCGCAATACcaca > 2:999594/1‑96 (MQ=255) ccAGATTTGAATGACGCCTACGTAAACATTTATCACTGACGGAAGAGTCTCTCTGGCAAAAATGTGCACAAAAACTTATTACCGCAATTCCACATATTcc < 2:1728822/100‑1 (MQ=255) ccAGATTTGAATGACGCCTACGTAAACATTTATCACTGACGGAAGAGTCTCTCTGGCAAAAATGTGCACAAAAACTTATTACCGCAATTCCACATATTcc > 2:2295305/1‑100 (MQ=255) aGATTTGAATGACGCCTACGTAAACATTTATCACTGACGGAAGAGTCTCTCTGGCAAAAATGTGCACAAAAACTTATTGCCGTAATTCCACATATTCCAg < 2:1437967/100‑1 (MQ=255) tttGAATGACGCTTACGTAAACATTTATCACTGACGGAAGAGTCTCTCTGGCAAAAATGTGCACAAAAACTTATTACCGCATTTCCACATATTCCAGAAt < 2:16652/100‑1 (MQ=255) tttGAATGACGCCTACGTAAACATTTATCACTGACGGAAGAGTCTCTCTGGCAAAAATGTGCACAAAAACTTATTACCGCAATTCCCCATATTCCAGAAt < 1:2040161/100‑1 (MQ=255) tGAATGACGCCTACGTAAACATTTATCACTGACGGAAGAGTCTCTCTGGCAAAAATGTGCACAAAAACTTATTACCGCAATTCCACATATTCCAGAATgg > 1:2402279/1‑100 (MQ=255) aTGACGCCTACGTAAACATTTATCACTGACGGAAGAGTCTCTCTGGCAAAAATGTGCACAAAAACTTATTACCGCAATTCCACATATTCCAGAATggcgg > 1:1947304/1‑100 (MQ=255) gACGCCTACGTAAACATTTATCACTTACGTAAGAGTCTCTCTGGCAAAAATGTGCACAAAAACTTATTACCGCAATTCCACATATTCCAGAATGGCGGCa < 2:1749633/100‑1 (MQ=255) cGCCTACGTAAACATTTATCACTGACGGAAGAGTCTCTCTGGCAAAAATGTGCACAAAAACTTATTACCGCAATTCCACATATTCCAGAATGGCGGCAAc < 1:1607897/100‑1 (MQ=255) cGCCTACGTAAACATTTATCACTGACGGAAGAGTCTCTCTGGCAAAAATGTGCACAAAAACTTATTACCGCAATTCCACATATTCCAGAAGGGCGGCa < 2:1445755/98‑1 (MQ=255) ccTACGTAAACATTTATCACTGACGGAAGAGTCTCTCTGGCAAAAATGTGCACAAAAACTTATTACCGCAATTCCACATATTCCAGAATGGCGGCAAGCa > 2:1838999/1‑100 (MQ=255) ccTACGTAAACATTTATCACTGACGGAAGAGTCTCTCTGGCAAAAATGTGCACAAAAACTTATTACCGCAATTCCACATATTCCAGAATGGCGGCAACCa > 2:1711083/1‑100 (MQ=255) tACGTAAACATTTATCACTGACGGAAGCGTCTCTCTGGCAAAAATGTGCACAAAAACTTATTACCGCAATTCCAAATATTCCAGAATGGCGGCAACAAtt > 1:1167745/1‑100 (MQ=255) tACGTAAACATTTATCACTGACGGAAGAGTTTCTCTGGCAAAAATGTGCACAAAAACTTATTACCGCAATTCCACATATTCCAGAATGGCGGCAACCa < 1:986139/98‑1 (MQ=255) tACGTAAACATTTATCACTGACGGAAGAGTCTCTCTGGCAAAAATGTGCACAAAAACTTATTACCGCAATTCCACATATTCCAGAATGGCGGCAACCAtt > 2:2287192/1‑100 (MQ=255) cGTAAACATTTATCACTGACGGAAGAGTCTCTCTGGCAAAAATGTGCACAAAATCTTATTACCGCAATTCCACATATTCCAGAATGGCGGCAACCATTaa < 2:1936388/100‑1 (MQ=255) tAAACATTTATCACTGACGGAAGAGTCTCTCTGGCAAAAATGTGCACAAAAACTTATTACCGCAATTCCACATATTCCAGAATGGCGGCAACCATTAAtt > 2:474638/1‑100 (MQ=255) tAAACATTTATCACTGACGGAAGAGTCTCTCTGGCAAAAATGTGCACAAAAACTTATTACCGCAATTCCACATATTCCAGAATGGCGGCAACCATTAAtt > 1:1907075/1‑100 (MQ=255) aaaCATTTATCACTGACGGAAGAGTCTCTCTGGCAAAAATGTGCACAAAAACTTATTACCGCAATTCCACATATTCCAGAATGGCGGCAACCATTAATTg < 2:934402/100‑1 (MQ=255) aaCATTTATCACTGACGGAAGAGTCTCTCTGGCAAAAATGTGCACAAAAACTTATTACCGCAATTCCACATATTCCAGAATGGCGGCAACCATTAATTg > 2:821196/1‑99 (MQ=255) aTTTATCACTGACGGAAGAGTCTCTCTGGCAAAAATGTGCACAAAAACTTATTACCGCAATTCCACATATTCCAGAATGGCGGCAACCATTAATTGCttt > 2:1607666/1‑100 (MQ=255) aTTTATCACTGACGGAAGAGTCTCTCTGGCAAAAATGTGCACAAAAACTTATTACCGCAATTCCACATATTCCAGAATGGCGGCAACCATTAATTGCttt < 1:2070016/100‑1 (MQ=255) aTTTATCACTGACGGAAGAGTCTCTCTGGCAAAAATGTGCACAAAAACTTATTACCGCAATTCCACATATTCCAGAATGGCGGCAACCATTAATTGCttt < 1:786524/100‑1 (MQ=255) tttATCACTGACGGAAGAGTCTCTCTGGCAAAAATGTGCACAAAAACTTATTACCGCAATTCCACATATTCCAGAATGGCGGCAACCATTAATTGCTTTg < 2:87076/100‑1 (MQ=255) tttATCACTGACGGAAGAGGCTCTCGTGCAAAAATGTGCACAAAAACTTATTACCGCAATTCCACATATTCCAGAATGGCTGCTACCATTAATTGCTTTg < 2:487119/100‑1 (MQ=255) ttATCACTGACGGAAGAGTCTCTCTTGCAAAAATGTGCACAAAAACTTATTACCGCAATTCCACATATTCCAGAATGGCGGCAACCATTAATTGCTTTGt < 1:58827/100‑1 (MQ=255) cACTGACGGAAGAGTCTCTCTGGCAAAAATGTGCACAAAAACTTATTACCGCAATTCCACATATTCCAGAATGGCGGCAACCATTAATTGCTTTGttatt > 1:1598993/1‑100 (MQ=255) cACTGACGGAAGAGTCTCTCTGGCAAAAATGTGCACAAAAACTTATTACCGCAATTCCACATATTCCAGAATGGCGGCAACCATTAATTGCTTTGttatt > 1:1469365/1‑100 (MQ=255) aCTGACGGAAGAGTCTCTCTGGCAAAAATGTGCACAAAAACTTATTACCGCAATTCCACATATTCCAGAATGGCGGCAACCATTAATTGCTTTGttatta > 1:1036912/1‑100 (MQ=255) tGACGGAAGAGTCTCTCTGGCAAAAATGTGCACAAAAACTTATTACCGCAATTCCACATATTCCAGAATGGCGGCAACCATTAATTGCTTTGTTATTAc < 2:2810042/99‑1 (MQ=255) ggAAGAGTCTCTCTGGCAAAAATGTGCACAAAAACTTATTACCGCAATTCCACATATTCCAGAATGGCAGCAACCATTAATTGCTTTGTTATTACCCGaa > 1:2970618/1‑100 (MQ=255) gAAGAGTCTCTCTGGCAAAAATGTGCACAAAAACTTATTACCGCAATTCCACATATTCCAGAATGGCGGCAACCATTAATTGCTTTGTTATTACCCGaaa > 2:415419/1‑100 (MQ=255) aatagactatctGGCAAAAATGTGCACAAAAACTTATTACCGCAATTCCACATATTCCAGAATGGCGGCAACCATTAATTGCTTTGTTATTACCCGaaaa < 2:2096459/91‑1 (MQ=255) aaGAGTCTCTCTGGCAAAAATGTGCACAAAAACTTATTACCGCAATTCCACATATTCCAGAATGGCGGCAACCATTAATTGCTTTGTTATTACCCGaaaa < 1:2963400/100‑1 (MQ=255) gagTCTCTCTGGCAAAAATGTGCACAAAAACTTATTACCGCAATTCCACATATTCCAGAATGGCGGCAACCATTAATTGCTTTGTTATTACCCGAAAAAc < 2:53027/100‑1 (MQ=255) agTCTCTCTGGCAAAAATGTGCACAAAAACTTATTACCGCAATTCCACATATTCCAGAATGGCGGCAACCATTAATTGCTTTGTTATTACCCGAAAAAcc < 2:2691619/100‑1 (MQ=255) agTCTCTCTGGCAAAAATGTGCACAAAAACTTATTACCGCAATTCCACATATTCCAGAATGGCGGCAACCATTAATTGCTTTGTTATTACCCGAAAAAcc < 1:220269/100‑1 (MQ=255) gTCTCTCTGGCAAAAATGTGCACAAAAACTTATTACCGCAATTTCACATATTCCAGAATGGCGGCAACCATTAATTGCTTTGTTATTACTCGAAAAACCa > 2:664875/1‑100 (MQ=255) ctctctGGCAAAAATGTGCACAAAAACTTATTACCGCAATTCCACATATTCCAGAATGGCGGCAACCATTAATTGCTTTGTTATTACCCGAAAAACCAGa > 1:2271735/1‑100 (MQ=255) ctGGCAAAAATGTGCACAAAAACTTATTACCGCAATTCCACATATTCCAGAATGGCGGCAACCATTAATTGCTTTGTTATTACCCGAAAAACCAGAAAt > 1:428362/1‑99 (MQ=255) tGGCAAAAATGTGCACAAAAACTTATTGCCGCAATTCCACATATTCCAGAATGGCGGCAACCATTAATTGCTTTGTTATTACCCGAAAAACCAGAAATTg > 2:231359/1‑100 (MQ=255) ggCAAAAATGTGCACAAAAACTTATTACCGCAATTCCACATATTCCAGAATGGCGGCAACCATTAATTGCTTTGTTATTACCCGAAAAACCAGAAATTGc < 1:2378709/100‑1 (MQ=255) ggCAAAAATGTGCACAAAAACTTATTACCGCAATTCCACATATTCCAGAATGGCGGCAACCATTAATTGCTTTGTTATTACCCGAAAAACCAGAAATTGc < 1:1741868/100‑1 (MQ=255) gCAAAAATGTGCACAAAAACTTATTACCGCAATTCCACATATTCCAGAATGGCGGCAACCATTAATTGCTTTGTTATTACCCGAAAAACCAGAAATTGca < 2:563927/100‑1 (MQ=255) gCAAAAATGTGCACAAAAACTTATTACCGCAATTCCACATATTCCAGAATGGCGGCAACCATTAATTGCTTTGTTATTACCCGAAAAACCAGAAATTGca < 2:2781127/100‑1 (MQ=255) gCAAAAATGTGCACAAAAACTTATTACCGCAATTCCACATATTCCAGAATGGCGGCAACCATTAATTGCTTTGTTATTACCCGAAAAACCAGAAATTGca < 2:1569659/100‑1 (MQ=255) gCAAAAATGTGCACAAAAACTTATTACCGCAATTCCACATATTCCAGAATGGCGGCAACCATTAATTGCTTTGTTATTACCCGAAAAACCAGAAATTGca > 1:2083620/1‑100 (MQ=255) gCAAAAATGTGCACAAAAACTTATTACCGCAATTCCACATATTCCAGAATGGCGGCAACCATTAATTGCTTTGTTATTACCCGAAAAACCAGAAATTGc < 1:774653/99‑1 (MQ=255) ctaaaaTGTGCACAAAAACTTATTATCGTATTTCCGTATAATCCAGAATGGCGGCAATCATTACTTGCTTTGTTATTACCCGAAAAACCAGAAATTGcac < 2:1364447/98‑1 (MQ=255) cAAAAATGTGCACAAAAACTTATTACCGCAATTCCACATATTCCAGAATGGCGGCAACCATTAATTGCTTTGTTATTACCCGAAAAACCAGAAATTGcac < 1:425326/100‑1 (MQ=255) gtgCACAAAAACTTATTACCGCAATTCCACATATTCCAGAATGGCGGCAACCATTAATTGCTTTGTTATTACCCGAAAAACCAGAAATTGCACATGAAAt > 1:1885557/1‑100 (MQ=255) gCACAAAAACTTATTACCGCAATTCCACATATTCCAGAATGGCGGCAACCATTAATTGCTTTGTTATTACCCGAAAAACCAGAAATTGCACATGAAATTg > 2:1334477/1‑100 (MQ=255) gCACAAAAACTTATTACCGCAATTCCACATATTCCAGAATGGCGGCAACCATTAATTGCTTTGTTATTACCCGAAAAACCAGAAATTGCACATGAAATTg < 1:2482154/100‑1 (MQ=255) gCACAAAAACTTATTACCGCAATTCCACATATTCCAGAATGGCGGCAACCATTAATTGCTTTGTTATTACCCGAAAAACCAGAAACTGCACATGAAATTg > 2:691355/1‑100 (MQ=255) cacaAAAACTTATTACCGCAATTCCACATATTCCAGAATTGCGGCAACCATTAATTGCTTTGTTATTACCCGAAAAACCAGAAATTGCACATGAAATTGc > 2:151435/1‑100 (MQ=255) cacaAAAACTTATTACCGCAATTCCACATATTCCAGAATGGCGGCAACCATTAATTGCTTTGTTATTACCCGAAAAACCAGAAATTGCACATGAAATTGc < 1:1304745/100‑1 (MQ=255) cacaAAAACTTATTACCGCAATTCCACATATTCCAGAATGGCGGCAACCATTAATTGCTTTGTTATTACCCGAAAAACCAGAAATTGCACATGAAATTGc > 2:176574/1‑100 (MQ=255) cacaAAAACTTATTACCGCAATTCCACATATTCCAGAATGGCGGCAACCATTAATTACTTAATTAATACCCGAAAAACCAGAAATTGCACATGAAATTGc > 1:64904/1‑100 (MQ=255) acaAAAACTTATTACCGCAATTCCACATATTCCAGAATGGCGGCAACCATTAATTGCTTTGTTATTACCCGAAAAACCAGAAATTGCACATGAAATTGcc > 2:1405780/1‑100 (MQ=255) acaAAAACTTATTACCGCAATTCCACATATTCCAGAATGGCGGCAACCATTAATTGCTTTGTTATTACCCGAAAAACCAGAAATTGCACATGAAATTAcc > 1:2197693/1‑100 (MQ=255) aaaaCTTATTACCGCAATTCCACATATTCCAGCATGGCGGCAACCATTAATTGCTTTGTTATTACCCGAAAAACCAGAAATTGCACATGAAATTGCCCAg > 1:468507/1‑100 (MQ=255) aaaaCTTATTACCGCAATTCCACATATTCCAGAATGGCGGCAACCATTAATTGCTTTGTTATTACCCGAAAAACCAGAAATTGCACATGAAATTGCCCAg > 2:2799181/1‑100 (MQ=255) aaaCTTATTACCGCAATTCCACATATGCCAGAATGGCGGCAACCATTAATTGCTTTGTTATTACCCGAAAAACCAGAAATTGCACATGAAATTGCCCAg < 2:1337878/99‑1 (MQ=255) aaCTTATTACCGCAATTCCACATATTCCAGAATGGCGGCAACCATTAATTGCTTTGTTATTACCCGAAAAACCAGAAATTGCACATGAAATTGCCCAGCg < 2:1397434/100‑1 (MQ=255) aCTTATTACCGCAATTCCACATATTCCAGAATGGCGGCAACCATTAATTGCTTTGTTATTACCCGAAAAACCAGAAATTGCACATGAAATTGCCCAGCGt > 1:2245451/1‑100 (MQ=255) cTTATTACCGCAATTCCACATATTCCAGAATGGCGGCAACCATTAATTGCTTTGTTATTACCCGAAAAACCAGAAATTGCACATGAAATTGCCCAGCGTc > 1:2289217/1‑100 (MQ=255) cTTATTACCGCAATTCCACATATTCCAGAATGGCGGCAACCATTAATTGCTTTGTTATTACCCGAAAAACCAGAAATTGCACATGAAATTGCCCAGCGTc > 1:1377512/1‑100 (MQ=255) tATTACCGCAATTCCACAAATTCCAGAATGGCGGCAACCATTAATTGCTTTGTTATTACCCGAAAAACCAGAAATTGCACATGAAATTTCCCAGCGTCTa > 2:2817243/1‑100 (MQ=255) aTTACCGCAATTCCACATATTCCAGAATGGCGGCAACCATTAATTGCTTTGTTATTACCCGAAAAACCAGAAATTGCACATGAAATTGCCCAGCGTCTAc < 1:1055670/100‑1 (MQ=255) | TACTTGGAGCAATATTCCAGATTTGAATGACGCCTACGTAAACATTTATCACTGACGGAAGAGTCTCTCTGGCAAAAATGTGCACAAAAACTTATTGCCGCAATTCCACATATTCCAGAATGGCGGCAACCATTAATTGCTTTGTTATTACCCGAAAAACCAGAAATTGCACATGAAATTGCCCAGCGTCTAC > NC_000913/2197269‑2197461 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |