Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,235,656 T→C 100% I131T (ATC→ACC)  preA → dihydropyrimidine dehydrogenase, NADH‑dependent, subunit C

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,235,6560TC97.5% 378.9 / ‑2.1 115I131T (ATC→ACC) preAdihydropyrimidine dehydrogenase, NADH‑dependent, subunit C
Reads supporting (aligned to +/- strand):  ref base T (1/2);  new base C (60/52);  total (61/54)
Fisher's exact test for biased strand distribution p-value = 6.00e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.17e-01

Only 100 of 115 total aligned reads displayed.

CCCGGACAAAGTATTGATCGCTTCGATCATGGGGGAAAATGAGCAGCAATGGGAGGAGCTGGCGCGCCTGGTGCAAGAAGCTGGCGCGGATATGATCGAGTGTAACTTCTCCTGTCCGCAAATGACTTCTCATGCGATGGGTAGCGATGTCGGGCAAAGCCCGGAGCTGGTAGAAAAATATTGTCGGGCAG  >  NC_000913/2235561‑2235751
                                                                                               |                                                                                               
ctcGGACAAAGTATTGATCGCTTCGATCATGGGGGAAAATGAGCAGCAATGGGAGGAGCTGGCGCGCCTGGTGCAAGAAGCTGGCGCGGATATGACCGAg                                                                                             <  1:1559166/98‑1 (MQ=255)
cccGGACAAAGTATTGATCGCTTCGATCATGGGGGAAAATGAGCAGCAATGGGAGGAGCTGGCGCGCCTGGTGCAAGAAGCTGGCGCGGATATGACCGAg                                                                                             >  1:13690/1‑100 (MQ=255)
cccGGACAAAGTATTGATCGCTTCGATCATGGGGGAAAATGAGCAGAAATGGGAGGAGCTGGCGCGCCTGGTGCAAGAAGCTGGCGCGGATATGACCGAg                                                                                             <  1:2358818/100‑1 (MQ=255)
cccGGACAAAGTATTGATCGCTTCGATCATGGAGGAACATGAGCAGCAATGGGAGGAGCAGGCGCGCCTGGTGCAAGAACCTGGCGCGGATATGACCGAg                                                                                             >  2:2733498/1‑100 (MQ=255)
  cGGACAAAGTATTGATCGCTTCGATCATGGGGGAAAATGAGCAGCAATGGGAGGAGCTGGCGCGCCTGGTGCAAGAAGCTGGCGCGGATATGACCGAgtg                                                                                           >  1:1227671/1‑100 (MQ=255)
   ggACAAAGTATTGATCGCCTCGATCATGGGGGAAAATGAGCAGCAATGGGAGGAGCTGGCGCGCCTGGTGCAAGAAGCTGGCGCGGATATGACCGAgtgt                                                                                          >  1:1813570/1‑100 (MQ=255)
    gACAAAGTATTGATCGCTTCGATCATGGGGGAAAATGAGCAGCAATGGGAGGAGCTGGCGCGCCTGGTGCAAGAAGCTGGCGCGGATATGACCGAGTGTa                                                                                         <  1:659782/100‑1 (MQ=255)
    gACAAAGTATTGATCGCTTCGATCATGGGGGAAAATCAGCAGCAATGGGAGGAGCTGGCGCGCCTGGTGCAAGAAGCTGGCGCGGATATGACCGAGTGTa                                                                                         <  1:2133449/100‑1 (MQ=255)
      cAAAGTATTGATCGCTTCGATCATGGGGGAAAATGAGCAGCAATGGGAGGAGCTGGCGCGCCTGGTGCAAGAAGCTGGCGCGGATATGACCGAGTGTAAc                                                                                       <  1:1101472/100‑1 (MQ=255)
      cAAAGTATTGATCGCTTCGATCATGGGGGAAAATGAGCAGCAATGGGAGGAGCTGGCGCGCCTGGTGCAAGAAGCTGGCGCGGATATGACCGAGTGTAAc                                                                                       <  1:688802/100‑1 (MQ=255)
       aaaGTATTGATCGCTTCGATCATGTGGGAAAATGAGCAGCAATGGGAGGAGCTGGCGCGCCTGGTGCAAGAAGCTGGCGCGGATATGACCGAGTGTAAc                                                                                       <  1:238661/99‑1 (MQ=255)
        aattATTGATCGCTTCGATCATGGGGGAAAATGAGCAGCATTGGGAGGATCTGGCGCGCCTGGTGCAAGAAGCTGGCGCGGATATGACCGAGTGTAACt                                                                                      <  2:1722825/96‑1 (MQ=255)
         aGTATTGATCGCTTCGATCATGGGGGAAAATGAGCAGCAATGGGAGGAGCTGGCGCGCCTGGTGCAAGAAGCTGGCGCGGATATGACCGAGTGTAACTtc                                                                                    <  2:1536565/100‑1 (MQ=255)
            aTTGATCGCTTCGATGATGGGGGAAAATGAGCAGCAATGGGAGGAGCTGGCGCGCCTGGTGCAAGATGCTGGCGCGGATATGACCGAGTGTAACTTCTcc                                                                                 <  2:2975579/100‑1 (MQ=255)
            aTTGATCGCTTCGATCATGGGGGAAAATGAGCAGCAATGGGAGGAGCTGGCGCGCCTGGTGCAAGAAGCTGGCGCGGATATGACCGAGTGTAACTTCTcc                                                                                 <  2:793896/100‑1 (MQ=255)
            aTTGATCGCTTCGATCATGGGGGAAAATGAGCAGCAATGGGAGGAGCTGGCGCGCCTGGTGCAAGAAGCTGGCGCGGATATGACCGAGTGTAACTTCTcc                                                                                 >  2:1569271/1‑100 (MQ=255)
            aTTGATCGCTTCGATCATGGGGGAAAATGAGCAGCAATGGGAGGAGCTGGCGCGCCTGGTGCAAGAAGCTGGCGCGGATATGACCGAGTGTAACTTCTcc                                                                                 >  2:1569873/1‑100 (MQ=255)
            aTTGATCGCTTCGATCATGGGGGAAAATGAGCAGCAATGGGAGGAGCTGGCGCGCCTGGTGCAAGAAGCTGGCGCGGATATGACCGAGTGTAACTTCTcc                                                                                 >  2:1604069/1‑100 (MQ=255)
            aTTGATCGCTTCGATCATGGGGGAAAATGAGCAGCAATGGGAGGAGCTGGCGCGCCTGGTGCAAGAAGCTGGCGCGGATATGACCGAGTGTAACTTCTcc                                                                                 <  2:2909045/100‑1 (MQ=255)
             ttGATCGCTTCGATCATGGGAGAAAATCAGCAGCAATGGGAGGAGCTGGCGCGCCTGGTGCAAGAAGCTGGCGCGGATATGACCGAGTGTAACTTCTCCt                                                                                >  2:1322383/1‑100 (MQ=255)
              tGATCGCTTCGATCATGGGGGAAAATGAGCAGCAATGGGAGGAGCTGGCGCGCCTGGTGCAAGAAGCTGGCGCGGATATGACCGAGTGTAACTTCTCCTg                                                                               >  2:1970486/1‑100 (MQ=255)
               gATCGCTTCGATCATGGGGGAAAATGAGCAGCAATGGGAGGAGCTGGCGCGCCTGGTGCAAGAAGCTGGCGCGGATATGACCGAGTGTAACTTCTCCTGt                                                                              >  2:1504860/1‑100 (MQ=255)
                aTCGCTTCGATCATGGGGGAAAATGAGCAGCAATGGGAGGAGCTGGCGCGCCTGGTGCAAGAAGCTGGCGCGGATATGACCGAGTGTAACTTCTCCTGTc                                                                             <  1:2156198/100‑1 (MQ=255)
                aTCGCTTCGATCATGGGGGAAAATGAGCAGCAATGGGAGGAGCTGGCGCGCCTGGTGCAAGAAGCTGGCGCGGATATGACCGAGTGTAACTTCTCCTGTc                                                                             <  1:1267807/100‑1 (MQ=255)
                aTCGCTTCGATCATGGGGGAAAATGAGCAGCAATGGGAGGAGCTGGCGCGCCTGGTGCAAGAAGCTGGCGCGGATATGACCGAGTGTAACTTCTCCTGTc                                                                             >  2:2089829/1‑100 (MQ=255)
                aTCGCTTCGATCATGGGGGAAAATGAGCAGCAATGGGAGGAGCTGGCGCGCCTGGTGCAAGAAGCTGGCGCGGATATGACCGAGTGTAACTTCTCCTGTc                                                                             >  2:1257302/1‑100 (MQ=255)
                 tCGCTTCGATCATGGGGGAAAATGAGCAGCAATGGGAGGAGCTGGCGCGCCTGGTGCAAGAAGCTGGCGCGGATATGACCGAGTGTAACTTCTCCTGTcc                                                                            <  1:1980367/100‑1 (MQ=255)
                  cGCTTCGATCATGGGGGAAAATGAGCAGCAATGGGAGGAGCTGGCGCGCCTGGTGCAAGAAGCTGGCGCGGATATGACCGAGTGTAACTTCTCCTGTCCg                                                                           >  2:1989347/1‑100 (MQ=255)
                    cTTCGATCATGGGGGAAAATGAGCAGCAATGGGAGGAGCTGGCGCGCCTGGTGCAAGAAGCTGGCGCGGATATGACCGAGTGTAACTTCTCCTGTCCGCa                                                                         <  1:2112258/100‑1 (MQ=255)
                      tCGATCATGGGGGAAAATGAGCAGCAATGGGAGGAGCTGGCGCGCCTGGTGCAAGAAGCTGGCGCGGATATGACCGAGTGTAACTTCTCCTGTCCGCaaa                                                                       <  2:2667499/100‑1 (MQ=255)
                        gATCATGGGGGAAAATGAGCAGCAATGGGAGGAGCTGGCGCGCCTGTTGCAAGAAGCTGGCGCGGATATGACCGAGTGTAACTTCTCCTGTCCGCAAATg                                                                     >  1:1349867/1‑100 (MQ=255)
                        gATCATGGGGGAAAATGAGCAGCAATGGGAGGAGCTGGCGCGCCTGGTGCAAGAAGCTGGCGCGGATATGATCGAGTGTAACTTCTCCTGTCCGCAAATg                                                                     <  1:2753405/100‑1 (MQ=255)
                         aTCATGGGGGAAAATAAGCAGCAATGGGAAGAGCAGGCGAGCCTGGTTCAAGAAGCTGGCGCGGATATGACCGAGTGTAACTTCTCGTGTGCGCa                                                                         >  2:1088708/1‑95 (MQ=255)
                             tGGGGGAAAATGAGCAGCAATGGGAGGAGCTGGCGCGCCTGGTGCAAGAAGCTGGCGCGGATATAACAGAGTGTAACTTCTCCTGTCCGCAAATGACTtc                                                                >  1:614985/1‑100 (MQ=255)
                              gggggAAAATGAGCAGCAATGGGAGGAGCTGGCGCGCCTGGTGCAAGAAGCTGGCGCGGATATGACCGAGTGTAACTTCTCCTGTCCGCAAATGACTtct                                                               >  2:1167145/1‑100 (MQ=255)
                              gggggAAAATGAGCAGCAATGGGAGGAGCTGGCGCGCCTGGTGCAAGAAGCTGGCGCGGATATGACCGAGTGTAACTTCTCCTGTCCGCAAATGACTtct                                                               >  2:156735/1‑100 (MQ=255)
                              gggggAAAATGAGCAGCAATGGGAGGAGCTGGCGCGCCTGGTGCAAGAAGCTGGCGCGGATATGACCGAGTGTAACTTCTCCTGTCCGCAAATGACTtct                                                               >  2:893866/1‑100 (MQ=255)
                              gggggAAAATGAGCAGCAATGGGAGGAGCTGGCGCGCCTGGTGCAAGAAGCTGGCGCGGATATGACCGAGTGTAACTTCTCCTGTCCGCAAATGACTtct                                                               <  1:435073/100‑1 (MQ=255)
                                gggAAAATGAGCAGCAATGGGAGGAGCTGGCGCGCCTGGTGCAAGAAGCTGGCGCGGATATGACCGAGTGTAACTTCTCCTGTCCGCAAATGACTTCTCa                                                             >  2:314919/1‑100 (MQ=255)
                                  gAAAATGAGCAGCAATGGGAGGAGCTGGCGCGCCTGGTGCAAGAAGCTGGCGCGGATATGACCGAGTGTAACTTCTCCTGTCCGCAAATGACTTCTCATg                                                           <  2:2667788/100‑1 (MQ=255)
                                       tGAGCAGCAATGGGAGGAGCTGGCGCGCCTGGTGCAAGAAGCTGGCGCGGATATGACCGAGTGTAACTTCTCCTGTCCGCAAATGACTTCTCATGCGATg                                                      <  1:2793874/100‑1 (MQ=255)
                                       tGAGCAGCAATGGGAGGAGCTGGCGCGCCTGGTGCAAGAAGCTGGCGCGGATATGACCGAGTGTAACTTCTCCTGTCCGCAAATGACTTCTCATGCGATg                                                      <  2:2605473/100‑1 (MQ=255)
                                       tGAGCAGCAATGGGAGGAGCTGGCGCGCCGGGTGCGAGAAGCTGGCGCGGATATGACCGAGTGTAACTTCTCCTGTCCGCAAATGACTTCTCATGCGATg                                                      <  2:1896652/100‑1 (MQ=255)
                                        gAGCAGCAATGGGAGGAGCTGGCGCGCTTGGTGCAAGAAGCTGGCGCGGATATGACCGAGTGTAACTTCTCCTGTCCGCAAATGACTTCTCATGCGATgg                                                     <  1:245593/100‑1 (MQ=255)
                                           cagcaATGGGAGGAGCTGGCGCGCCTGGTGCCAGAAGCTGGCGCGGATATGACCGAGTGTAACTTCTCCTGTCCACAAATGACTTCTCATGCGATGGGTa                                                  >  1:1253205/1‑100 (MQ=255)
                                           cagcaATGGGAGGAGCTGGCGCGCCTGGTGCAAGAAGCTGGCGCGGATATGACCGAGTGTAACTTCTCCTGTCCGCAAATGACTTCTCATGCGATGGGTa                                                  >  1:1247615/1‑100 (MQ=255)
                                           caacaATGGGAGGAGCTGGCGCGCCTGGTGCAAGAAGCTGGCGCGGATATGACCGAGTGTAACTTCTCCTGTCCGCAAATGACTTCTCATGCGATGGGTa                                                  >  2:3023974/4‑100 (MQ=255)
                                            agcaATGGGAGGAGCTGGCGCGCCTGGTGCAAGAAGCTGGCGCGGATATGACCGAGTATAACTTCTCCTGTCCGCAAATGACTTCTCATGCGATGGGTAg                                                 >  1:1065184/1‑100 (MQ=255)
                                             gcaATGGGAGGAGCTGGCGCGCCTGGTGCAAGAAGCTGGCGCGGATATGACCGAGTGTAACTTCTCCTGTCCGCAAATGACTTCTCATGCGATGGGTAGc                                                >  1:3018154/1‑100 (MQ=255)
                                             gcaATGGGAGGAGCTGGCGCGCCTGGTGCAAGAAGCTGGCGCGGAAATGACCGAGTGTAACTTCTCCTGTCCGCAAATGACTTCTCATGCGATGGGTAGc                                                >  2:2434082/1‑100 (MQ=255)
                                              caATGGGAGTAGCTGGCGCGCCTGGTGTAATAAGCTGTCGCGGATATGATCGAGTGTAACTTCTCCTGTCCGCAAATGACTTCTCATGCGATGGGTAGCg                                               <  1:54486/100‑1 (MQ=255)
                                               aaTGGGAGGAGCTGGCGCGCCTGGTGCAAGAAGCTGGCGCGGATATGATCGAGTGTAACTTCTCCTGTCCGCAAATGACTTCTCATGCGATGGGTAGCGa                                              >  1:931234/1‑100 (MQ=255)
                                               aaTGGGAGGAGCTGGCGCGCCTGGTGCAAGAAGCTGGCGCGGATATGACCGAGTGTAACTTCTCCTGTCCGCAAATGACTTCTCATGCGATGGGTAGCg                                               >  2:2053025/1‑99 (MQ=255)
                                               aaTGGGAGGAGCTGGCGCGCCTGGTGCAAGAAGCTGGCGCGGATATGACCGAGTGTAACTTCTCCTGTCCGCAAATGACTTCTCATGCGATGGGTAGCGa                                              <  2:1833363/100‑1 (MQ=255)
                                                  gggAGGAGCTGGCGCGCCTGGTGCAAGAAGCTGGCGCGGATATGACCGAGTGTAACTTCTCCTGTCCGCAAATGACTTCTCATGCGATGGGTAGCGATg                                            >  1:2825736/1‑99 (MQ=255)
                                                    gaggagCTGGCGCGCCTGGTGCAAGAAGCTGGCGCGGATATGACCGAGTGTAACTTCTCCTGTCCGCAAATGACTTCTCATGCGATGGGTAGCGATGTCg                                         <  2:2224577/100‑1 (MQ=255)
                                                    gaggagCTGGCGCGCCTGGTGCAAGAAGCTGGCGCGGATATGACCGAGTGTAACTTCTCCTGTCCGCAAATGACTTCTCATGCGATGGGTAGCGATGTCg                                         <  1:550522/100‑1 (MQ=255)
                                                    cggtcatatccgcgcCTGGTGCAAGAAGCTGGCGCGGATATGACCGAGTGTAACTTCTCCTGTCCGCAAATGACTTCTCATGCGATGGGTAGCGATGTCg                                         <  2:1629029/90‑1 (MQ=255)
                                                      ggagCTGGCGCGCCTGGTGCAAGAAGCTGGCGCGGATATGACCGAGTGTAACTTCTCCTGTCCGCAAATGACTTCTCATGCGATGGGTAGCGATGTCggg                                       <  1:1718965/100‑1 (MQ=255)
                                                         ncTGGCGCGCCTGGTGCAAGAAGCTGGCGCGGATATGACCGAGTGTAACTTCTCCTGTCCGCAAATGACTTCTCATGCGATGGGTAGCGATGTCGGGCaa                                    >  2:2677342/2‑100 (MQ=255)
                                                         gCTGGCGCGCCTGGTGCAAGAAGCTGGCGCGGATATGACCGAGTGTAACTTCTCCTGTCCGCAAATGACTTCTCATGCGATGGGTAGCGATGTCGGGCaa                                    >  2:2162350/1‑100 (MQ=255)
                                                         gCTGGCGCGCCTGGTGCAAGAAGCTGGCGCGGATATGACCGAGTGTAACTTCTCCTGTCCGCAAATGACTTCTCATGCGATGGGTAGCGATGTCGGGCaa                                    >  2:2163809/1‑100 (MQ=255)
                                                           tGGCGCGCCTGGTGCAAGAAGCTGGCGCGGATATGACCGAGTGTAACTTCTCCTGTCCGCAAATGACTTCTCATGCGATGGGTAGCGATGTCGGGCAAAg                                  >  1:2350363/1‑100 (MQ=255)
                                                           tGGCGCGCCTGGTGCAAGAAGCTGGCGCGGATATGACCGAGTGTAACTTCTCCTGTCCGCAAATGACTTCTCATGCGATGGGTAGCGATGTCGGGCAAAg                                  >  2:1836688/1‑100 (MQ=255)
                                                            ggCGCGCCTGGTGCAAAAAGCTGGCGCGGATATGACCGAGTGTAACTTCTGCTGTCCGCAAATGACTTCTCATGCGATGAGTGACGATGACGGGCa                                     >  2:2787718/1‑96 (MQ=255)
                                                              cgcgcCTGGTGCAAGAAGCTGGCGCGGATATGACCGAGTGTAACTTCTCCTGTCCGCAAATGACTTCTCATGCGATGGGTAGCGATGTCGGGCAAAGccc                               >  2:2825515/1‑100 (MQ=255)
                                                              cgcgcCTGGTGCAAGAAGCTGGCGCGGATATGACCGAGTGTAACTTCTCCTGTCCGCAAATGACTTCTCATGCGATGGGTAGCGATGTCGGGCAAAGccc                               >  1:2312031/1‑100 (MQ=255)
                                                                cgcCTGGTGCAAGAAGCTGGCGCGGATATGACCGAGTGTAACTTCTCCTGTCCGCAAATGACTTCTCATGCGATGGGTAGCGATGTCGGGCAAAGCCCgg                             <  2:1410224/100‑1 (MQ=255)
                                                                 gcCTGGTGCAAGAAGCTGGCGCGGATATGACCGAGTGTAACTTCTCCTGTCCGTAAATGACTTCTCATGCGATGGGTAGCGATGTCGGGCAAAGCCCgg                             <  2:5542/99‑1 (MQ=255)
                                                                 gcCTGGTGCAAGAAGCTGGCGCGGATATGACCGAGTGTAACTTCTCCTGTCCGCAAATGACTTCTCATGCGATGGGTAGCGATGTCGGGCAAAGCCCGGa                            <  2:2254426/100‑1 (MQ=255)
                                                                     ggTGCAAGAAGCTGGCGCGGATATGACCGAGTGTAACTTCTCCTGTCCGCAAATGACTTCTCATGCGATGGGTAGCGATGTCGGGCAAAGCCCGGAGc                          <  2:2075642/98‑1 (MQ=255)
                                                                     ggTGCAAGAAGCTGGCGCGGATATGACCGAGTGTAACTTCTCCTGTCCGCAAATGACTTCTCATGCGATGGGTAGCGATGTCGGGCAAAGCCCGGAGCTg                        <  1:2799341/100‑1 (MQ=255)
                                                                     ggTGCAAGAAGCTGGCGCGGATATGACCGAGTGTAACTTCTCCTGTCCGCAAATGACTTCTCATGCGATGGGTAGCGATGTCGGGCAAAGCCCGGAGCTg                        >  2:2907359/1‑100 (MQ=255)
                                                                     ggTGCAAGAAGCTGGCGCGGATATGACCGAGTGTAACTTCTCCTGTCCGCAAATGACTTCTCATGCGATGGGTAGCGATGTCGGGCAAAGCCCGGAGCTg                        >  2:2236503/1‑100 (MQ=255)
                                                                     ggTGCAAGAAGCTGGCGCGGATATGACCGAGTGTAACTTCTCCTGTCCGCAAATGACTTCTCATGCGATGGGTAGCGATGTCGGGCAAAGCCCGGAGCTg                        >  1:1136073/1‑100 (MQ=255)
                                                                     ggTGCAAGAAGCTGGCGCGGATATGACCGAGTGTAACTTCTCCTGTCCGCAAATGACTTCTCATGCGATGGGTAGCGATGTCGGGCAAAGCCCGGAGCTg                        <  1:1983170/100‑1 (MQ=255)
                                                                      gTGCAAGAAGCTGGCGCGGATATGACCGAGTGTAACTTCTCCTGTCCGCAAATGACTTCTCATGCGATGGGTAGCGATGTCGGGCAAAGCCCGGAGCTgg                       <  1:2886859/100‑1 (MQ=255)
                                                                      gTGCAAGAAGCTGGCGCGGATATGACCGAGTGTAACTTCTCCTGTCCGCAAATGACTTCTCATGCGATGGGTAGCGATGTCGGGCAAAGCCCGGAGCTgg                       >  1:1328852/1‑100 (MQ=255)
                                                                       tGCAAGGAGCTTGCGCGGAGATGACCGTGAGTAGGTTCGCGTGTCCGCAAATGTCTTTTCATGCGATGGGGAGCTATGTCGGGCAAAGCCCGGAGCTGGt                      <  2:191070/100‑1 (MQ=255)
                                                                        gCAAGAAGCTGGCGCGGATATGACCGAGTGTAACTTCTCCTGTCCGCAAATGACTTCTCATGCGATGGGTAGCGATGTCGGGCAAAGCCCGGAGCTGGTa                     >  2:449205/1‑100 (MQ=255)
                                                                        gCAAGAAGCTGGCGCGGATATGACCGAGTGTAACTTCTCCTGTCCGCAAATGACTTCTCATGCGATGGGTAGCGATGTCGGGCAAAGCCCGGAGCTGGTa                     <  1:1464020/100‑1 (MQ=255)
                                                                        gCAAGAAGCTGGCGCGGATATGACCGAGTGTAACTTCTCCTGTCCGCAAATAACTTCTCATGCGAAGGGTAGAGATGTCGGGCAAAGCACGGAGCTGGTa                     >  1:498297/1‑100 (MQ=255)
                                                                         cAAGAAGCTGGCGCGGATATGACCGAGTGTAACTTCTCCTGTCCGCAAATGACTTCTCATGCGATGGGTAGCGATGTCGGGCAAAGCCCGGAGCTGGTAg                    <  2:479479/100‑1 (MQ=255)
                                                                          aagaagCTGGCGCGGATATGACCGAGTGTAACTTCTCCTGTCTGCAAATGACTTCTCATGCGATGGGTAGCGATGTCGGGCAAAGCCCGGAGCTGGTAg                    <  1:776665/99‑1 (MQ=255)
                                                                          aagaagCTGGCGCGGATATGACCGAGTGTAACTTCTCCTGTCCGCAAATGACTTCTCATGCGATGGGTAGCGATGTCGGGCAAAGCCCGGAGCTGGTAGa                   <  1:2468116/100‑1 (MQ=255)
                                                                          aagaagCTGGCGCGGATATGACCGAGTGTAACTTCTCCTGTCCGCAAATGACTTCTCATGCGATGGGTAGCGATGTCGGGCAAAGCCCGGAGCTGGTAGa                   <  1:81767/100‑1 (MQ=255)
                                                                           agaagCTGGCGCGGATATGACCGAGTGTAACTTCTCCTGTCCGCAAATGACTTCTCATGCGATGGGTAGCGATGTCGGGCAAAGCCCGGAGCTGGTAGaa                  >  1:2833353/1‑100 (MQ=255)
                                                                             aagCTGGCGCGGATATGACCGAGTGTAACTTCTCCTGTCCGCAAATGACTTCTCATGCGATGGGTAGCGATGTCGGGCAAAGCCCGGAGCTGGTAGaaaa                <  1:953833/100‑1 (MQ=255)
                                                                              agCTGGCGCGGATATGACCGAGTGTAACTTCTTCTGTCCGCAAATGACTTCTCATGCGATGGGTAGCGATGTCGGGCAAAGCCCGGAGCTGGTAGaaaaa               <  2:2131549/100‑1 (MQ=255)
                                                                              agCTGGCGCGGATATGACCGAGTGTAACTTCTCCTGTCCGCAAATGACTTCTCATGCGATGGGTAGCGATGTCGGGCAAAGCCCGGAGCTGGTAGaaaaa               >  1:524938/1‑100 (MQ=255)
                                                                                  ggCGCGGATATGACCGAGTGTAACTTCTCCTGTCCGCAAATGACTTCTCATGCGATGGGTAGCGATGTCGGGCAAAGCCCGGAGCTGGTAGAAAAATAtt           >  1:2433425/1‑100 (MQ=255)
                                                                                  ggCGCGGATATGACCGAGTGTAACTTCTCCTGTCCGCAAATGACTTCTCATGCGATGGGTAGCGATGTAGACCAAAGCCCGGAGCTGGTAGAAAAATAtt           >  2:2656614/1‑100 (MQ=255)
                                                                                    cgcgGATATGACCGAGTGTAACTTCTCCTGTCCGCAAATGACTTCTCATGCGATGGGTAGCGATGTCGGGCAAAGCCCGGAGCTGGTAGAAAAATATTGt         >  2:2091537/1‑100 (MQ=255)
                                                                                       ggATATGACCGAGTGTAACTTCTCCTGTCCGCAAATGACTTCTCATGCGATGGGTAGCGATGTCGGGCAAAGCCCGGAGCTGGTAGAAAAATATTGTCgg      <  1:2091560/100‑1 (MQ=255)
                                                                                       ggATATGACCGAGTGTAACTTCTCCTGTCCGCAAATGACTTCTCATGAGATGGGTAGCGATGTCGGGCAAAGCCCGGAGCTGGTAGAAAAATATTGTCgg      >  2:891953/1‑100 (MQ=255)
                                                                                        gATATGACCGAGTGTAACTTCTCCTGTCCGCAAATGACTTCTCATGCGATGGGTAGCGATGTCGGGCAAAGCCCGGAGCTGGTAGAAAAATATTGTCggg     >  2:1375937/1‑100 (MQ=255)
                                                                                        gATATGACCGAGTGTAACTTCTCCTGTCCGCAAATGACTTCTCATGCGATGGGTAGCGATGTCGGGCAAAGCCCGGAGCTGGTAGAAAAATATTGTCggg     <  2:1201881/100‑1 (MQ=255)
                                                                                          tatGACCGAGTGTAACTTCTCCTGTCCGCAAATGACTTCTCATGCGATGGGTAGCGATGTCGGGCAAAGCCCGGAGCTGGTAGAAAAATATTGTCGGGc    <  2:109372/99‑1 (MQ=255)
                                                                                           atGACCGAGTGTAACTTCTCCTGTCCGCAAATGACTTCTGATGCGATGGGTAGCGATGTCGGGCAAAGCCCGGAGCTGGTAGAAAAATATTGTCGGGCAg  <  2:393369/100‑1 (MQ=255)
                                                                                           atGACCGAGTGTAACTTCTCCTGTCCGCAAATGACTTCTCATGCGATGGGTAGCGATGTCGGGCAAAGCCCGGAGCTGGTAGAAAAATATTGTCGGGCAg  <  1:670923/100‑1 (MQ=255)
                                                                                               |                                                                                               
CCCGGACAAAGTATTGATCGCTTCGATCATGGGGGAAAATGAGCAGCAATGGGAGGAGCTGGCGCGCCTGGTGCAAGAAGCTGGCGCGGATATGATCGAGTGTAACTTCTCCTGTCCGCAAATGACTTCTCATGCGATGGGTAGCGATGTCGGGCAAAGCCCGGAGCTGGTAGAAAAATATTGTCGGGCAG  >  NC_000913/2235561‑2235751

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: