Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,250,210 T→A 32.2% V165E (GTG→GAG)  yeiH → UPF0324 family inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,250,2100TA32.2% 151.9 / 97.6 114V165E (GTG→GAG) yeiHUPF0324 family inner membrane protein
Reads supporting (aligned to +/- strand):  ref base T (38/39);  new base A (28/9);  total (66/48)
Fisher's exact test for biased strand distribution p-value = 8.76e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 116 total aligned reads displayed.

ACACCAGCTGGTTGATCGGTGCCGGTAGCAGTATCTGTGGTGCTGCCGCGGTACTGGCGACTGAGCCGGTAGTGAAAGCGGAAGCCAGTAAAGTAACCGTGGCTGTTGCAACCGTTGTTATCTTCGGGACCGTCGCGATTTTCCTCTACCCGGCGATATATCCGCTGATGTCTCAATGGTTTAGTCCGGAAACTT  >  NC_000913/2250111‑2250305
                                                                                                   |                                                                                               
ncacCAGCTGGTTGATCGGTGCCGGTAGCAGTATCTGTGGTGCTGCCGCGGTACTGGCGACTGAGCCGGTAGTGAAAGCGGAAGCCAGTAAAGTAACCGt                                                                                                 >  2:2312741/2‑100 (MQ=255)
   ccAGCTGGTTGATCGGTGTCGGTAGCAGTATCTGTGGTGCTGCCGCGGTACTGGCGACTGAGCCGGTAGTGAAAGCGGAAGCCAGTAAAGTAACCGAGGc                                                                                              <  1:1047272/100‑1 (MQ=255)
   ccAGCTGGTTGATCGGTGTCGGTAGCAGTATCTGTGGTGCTGCCGCGGTACTGGCGACTGAGCCGGTAGTGAAAGCGGAAGCCAGTAAAGTAACCGAGGc                                                                                              <  1:2819310/100‑1 (MQ=255)
   ccAGCTGGTTGATCGGTGCCTGTAGCAGTATCTTTGGTGCTGCCGCGGTACTGGCGACTGAGCCGGTAGTGAAAGCGGAAGCCAGTAAAGTAACCGTGGc                                                                                              <  1:189014/100‑1 (MQ=255)
   ccAGCTGGTTGATCGGTGCCGGTAGCAGTATCTGTGGTGCTGCCGCGGTACTGGCGACTGAGCCGGTAGTGAAAGCGGAATCCAGTAAAGTAACCGAgg                                                                                               >  2:875399/1‑99 (MQ=255)
   ccAGCTGGTTGATCGGTGCCGGTAGCAGTATCTGTGGTGCTGCCGCGGTACTGGCGACTGAGCCGGTAGTGAAAGCGGAAGCCAGTAAAGTAACCGAGGc                                                                                              <  2:2438777/100‑1 (MQ=255)
   ccAGCTGGTTGATCGGTGCCGGTAGCAGTATCTGTGGTGCTGCCGCGGTAATGGCGACTGAGCCGGTAGTGAAAGCGGAAGCCAGTAAAGTAACAGt                                                                                                 >  1:883513/1‑97 (MQ=255)
    cAGCTGGTTGATCGGTGCCGGTAGCAGTATCTGTGGTGCTGCCGCGGTACTGGCGACTGAGCCGGTAGTGAAAGCGGAAGCCAGTAAAGTAACCGTGGCt                                                                                             <  1:1777596/100‑1 (MQ=255)
        tGGTTGATCGGTGCCGGTAGCAGTATCTGTGGTGCTGCCGCGGTACTGGCGACTGAGCCGGTAGTGAAAGCGGAAGCCAGTAAAGTAACCGTGGCTGTTg                                                                                         <  1:1615995/100‑1 (MQ=255)
         ggTTGATCGGTGCCGGTAGCAGTATCTGTGGTGCTGCCGCGGTACTGGCGACTGAGCCGGTAGTGAAAGCGGAAGCCAGTAAAGTAACCGAGGCTGTTGc                                                                                        >  1:2578498/1‑100 (MQ=255)
         ggTTGATCGGTGCCGGTAGCAGTATCTGTGGTGCTGCCGCGGTACTGGCGACTGAGCCGGTAGTGAAAGCGGAAGCCAGTAAAGTAACCGAGGCTGTTGc                                                                                        >  2:2712630/1‑100 (MQ=255)
         ggTTGATCGGTGCCGGTAGCAGTATCTGTGGTGCTGCCGCGGTACTGGCGACTGAGCAGGTAGTGAAAGCGGAAGCCAGTAAAGTAACCGTGGATGTTGc                                                                                        >  1:1930601/1‑100 (MQ=255)
          gTTGATCGGTGCCGGTAGCAGTATCTGTGGTGCTGCCGCGGTACTGGCGACTGAGCTGGTAGTGAAAGCGGAAGCCAGTAAAGTAACCGTGGCTGTTGCa                                                                                       >  2:1937203/1‑100 (MQ=255)
           ttGATCGGTGCCGGTAGCAGTATCTGTGGTGCTGCCGCGGTACTGGCGACTGAGCCGGTAGTGAAAGCGGAAGCCAGTAAAGTAACCGAGGCTGTTGCaa                                                                                      >  2:2159496/1‑100 (MQ=255)
            tGATCGGTGCCGGTAGCAGTATCTGTGGTGCTGCCGCGGTACTGGCGACTGAGCCGGTAGTGAAAGCGGAAGCCAGTAAAGTAACCGTGGCTGTTGCAAc                                                                                     >  1:2982501/1‑100 (MQ=255)
             gATCGGTGCCGGTAGCAGTATCTGTGGTGCTGCCGCGGTACTGGCGACTGAGCCGGTAGTGAAAGCGGAAGCCAGTAAAGTAACCGTGGCTGTTGCAAcc                                                                                    >  2:1060520/1‑100 (MQ=255)
             gATCGGTGCCGGTAGCAGTATCTGTGGTGCTACCGCGGTACTGGCGACTGAGCCGGTAGTGAAAGCGGAAGCCAGTAAAGTAACCGAGGCTGTTGCAAcc                                                                                    >  2:1556458/1‑100 (MQ=255)
                  gTGCCGGTAGCAGTATCTGTGGTGCTGCCGCGGTACTGGCGATTGAGCCGGTAGTGAAAGCGGAAGCCAGTAAAGTAACCGTGGCTGTTGCAACCgttgt                                                                               <  1:787530/100‑1 (MQ=255)
                  gTGCCGGTAGCAGTATCTGTGGTGCTGCCGCGGTACTGGCGACTGAGCCGGTAGTGAAAGCGGAAGCCAGTAAAGTAACCGTGGCTGTTGCAACCgttgt                                                                               <  1:2558211/100‑1 (MQ=255)
                   tGCCGGTAGCAGTATCTGTGGTGCTGCCGCGGTACTGGCGACTGAGCCGGTAGTGAAAGCGGAAGCCAGTAAAGTAACCGTGGCTGTTGCAACCgttgtt                                                                              <  2:1236624/100‑1 (MQ=255)
                     ccGGTAGCAGTATTTTTGGTGCTGCCGCGGTACCGGCGACTGAGCCGGTAGTGAAAGCGGAAGCCAGTAAAGTAACCGTGGCTGTTGCAACAGTTGTTa                                                                             <  2:1585446/99‑1 (MQ=255)
                         tAGCAGTATCTGTGATGCTACAGAGGTAATAGCGACTGAGACGGTAGTGAAAGCGGAAGCCAGTAAAGTAACCGTGGCTGTTGCAACCGTTGTTATCTTc                                                                        >  2:750779/1‑100 (MQ=255)
                          aGCAGTATCTGTGGTGCTGCCGCGGTACTGGCGACTGAGCCGGTAGTGAAAGCGGAAGCCAGTAAAGTAACCGTGGCTGTTGCAACCGTTGTTATCTTCg                                                                       <  1:2802240/100‑1 (MQ=255)
                          aGCAGTATCTGTGGTGCTGCCGCGGTACTGGCGACTGAGCCGGTAGTGAAAGCGGAAGCCAGTAAAGTAACCGAGGCTGTTGCAACCGTTGTTATCTTCg                                                                       <  1:1479874/100‑1 (MQ=255)
                           gCAGTATCTGTGGTGCTGCCGCGGTACTGGCGACTGAGCCGGTAGTGAAAGCGGACGCCAGTAAAGTAACCGTGGCTGTTGCAACCGTTGTTATCTTctg                                                                      >  2:1073088/1‑98 (MQ=255)
                           gCAGTATCTGTGGTGCTGCCGCGGTACTGGCGACTGAGCCGGTAGTGAAAGCGGAAGCCAGTAAAGTAACCGTGGCTGTTGCAACCGTTGTTATCTTCg                                                                       >  1:1236698/1‑99 (MQ=255)
                            cAGTATCTGTGGTGCTGCCGCGGTACTGGCGACTGAGCCGGTAGTGAAAGCGGAAGCCAGTAAAGTAACCGTGGCTGTTGCAACCGTTGTTATCTTCggg                                                                     <  1:1937226/100‑1 (MQ=255)
                            cAGTATCTGTGGTGCTGCCGCGGTACTGGCGACTGAGCCGGTAGTGAAAGCGGAAGCCAGTAAAGTAACCGTGGCTGTTGCAACCGTTGTTATCTTCggg                                                                     <  1:2835998/100‑1 (MQ=255)
                            cAGTATCTGTGGTGCTGCCGCGGTACTGGCGACTGAGCCGGTAGTGAAAGCGGAAGCCAGTAAAGTAACCGTGGCTGTTGCAACCGTTGTTATCTTCggg                                                                     >  1:2354997/1‑100 (MQ=255)
                            cAGTATCTGTGGTGCTGCCGCGGTACTGGCGACTGAGCCGGTAGTGAAAGCGGAAGCCAGTAAAGTAACCGAGGCTGTTGCAACCGTTGTTATCTTCgg                                                                      <  2:2890965/99‑1 (MQ=255)
                              gTATCTGTGGTGCTGCCGCGGTACTGGCGACTGAGCCGGTAGTGAAAGCGGAAGCCAGTAAAGTAACCGTGGCTGTTGCAACCGTTGTTATCTTCGGGAc                                                                   >  2:1216319/1‑100 (MQ=255)
                              cgaTCTGTGGTGCTGCCGCGGTACTGGCGACTGAGCCGGTAGTGAAAGCGGAAGCCAGTAAAGTAACCGAGGCTGTTGCAACCGTTGTTATCTTCGGGAc                                                                   <  2:2608158/98‑1 (MQ=255)
                               tATCTGTGGTGCTGCCGCGGTACTGGCGACTGAGCCGGTAGTGAAAGCGGAAGCCAGTAAAGTAACCGAGGCTGTTGCAACCGTTGTTATCTTCGGGAcc                                                                  >  2:2519517/1‑100 (MQ=255)
                                 tCTGTGGTGCTGCCGCGGTACTGGCGACTGAGCCGGTAGTGAAAGCGGAAGCCAGTAAAGTAACCGTGGCTGTTGCAACCGTTGTTATCTTCGGGACCgn                                                                <  1:2517613/100‑2 (MQ=255)
                                 tCTGTGGTGCTGCCGCGGTACTGGCGACTGAGCCGGTAGTGAAAGCGGAAGCCAGTAAAGTAACCGAGGCTGTTGCAACCGTTGTTATCTTCGGGACCGt                                                                >  2:470368/1‑100 (MQ=255)
                                 gcTGTGGTGCTGCCGCGGTACTGGCGAGTGAGCCGGTAGGGAAAGCGGAAGCCAGTAAAGTAAGGGAGGCGGTTGGAAGCGTTGTTATATTc                                                                        >  2:2581716/2‑92 (MQ=255)
                                  cTGTGGTGCTGCCGCGGTACTGGCGACTGAGCCGGTAGTGAAAGCGGAAGCCAGTAAAGTAACCGTGGCTGTTGCAACCGTTGTTATCTTCGGGACCGTc                                                               >  2:45171/1‑100 (MQ=255)
                                  cTGTGGTGCTGCCGCGGTACTGGCGACTGAGCCGGTAGTGAAAGCGGAAGCCAGTAAAGTAACCGTGGCTGTTGCAACCGTTGTTATCTTCGGGACCGTc                                                               >  2:1067060/1‑100 (MQ=255)
                                  cTGTGGTGCTGCCGCGGTACTGGCGACTGAGCCGGTAGTGAAAGCGGAAGCCAGTAAAGTAACCGAGGCTGTTGCAACCGTTGTTATCTTCGGGACCGt                                                                >  2:372970/1‑99 (MQ=255)
                                   tgtgGTGCTGCCGCGGTACTGGCGACTGAGCCGGTAGTGAAAGCGGAAGCCAGTAAAGTAACCGAGGCTGTTGCAACCGTTGTTATCTTCGGGACCGTcg                                                              >  1:1736796/1‑100 (MQ=255)
                                    gtggtgCTGCCGCGGTACTGGCGACTGAGCCGGTAGTGAAAGCGGAAGCCAGTAAAGTAACCGAGGCTGTTGCAACCGTTGTTATCTTCGGGAcagatcg                                                             >  1:2608190/1‑94 (MQ=255)
                                     tggtgCTGCCGCGGTACTGGCGACTGAGCCGGTAGTGGAAGCGGAAGCCAGTAAAGTAACCGTGGCTGTTGCAACCGTTGTTATCTTCGGGATCGTcgcg                                                            >  2:1624911/1‑100 (MQ=255)
                                       gtgCTGCCGCGGTACTGGCGACTGAGCCGGTAGTGAAAGCGGAAGCCAGTAAAGTAACCGTGGCTGTTGCAACCGTTGTTATCTTCGGGACCGTcgcg                                                            >  2:2769392/1‑98 (MQ=255)
                                        tgctgcCGCGGTACTGGCGACTGAGCCGGTAGTGAAAGCGGAAGCCAGTAAAGTAACCGAGGCTGTTGCAACCGTTGTTATCTTCAGGACCGTCGCGAtt                                                         >  1:2174016/1‑100 (MQ=255)
                                         gctgcCGCGGTACTGGCGACTGAGCCGGTAGTGAAAGTGGAAGCCAGTAAAGTAACCGTGGCTGTTGCAACCGTTGTTATCTTCGGGACCGTCGCGAttt                                                        <  1:961353/100‑1 (MQ=255)
                                           tgcCGCGGTACTGGCGACTGAGCCGGTAGTGAAAGCGGAAGCCAGTAAAGTAACCGTGGCTGTTGCAACCGTTGTTATCTTCGGGACCGTCGCGATTTTc                                                      >  1:923148/1‑100 (MQ=255)
                                              cgcgGTACTGGCGACTGAGCCGGTAGTGAAAGCGGAAGCCAGTAAAGTAACCGTGGCTGTTGCAACCGTTGTTATCTTCGGGACCGTCGCGATTTTCctc                                                   <  1:802356/100‑1 (MQ=255)
                                                     cTGGCGACTGAGCCGGTAGTGAAAGCGGAAGCCAGTAAAGTAACCGTGGCTGTTGCAACCGTTGTTATCTTCGGGACCGTCGCGATTTTCCTCTACCCg                                             >  1:383713/1‑99 (MQ=255)
                                                      tGGCGACTGAGCCGGTAGTGAAAGCGGAAGCCAGTAAAGTAACCGTGGCTGTTGCAACCGTTGTTATCTTCGGGACCGTCGCGATTTTCCTCTACCCGGc                                           >  2:2315802/1‑100 (MQ=255)
                                                      tGGCGACTGAGCCGGTAGTGAAAGCGGAAGCCAGTAAAGTAACCGAGGCTGTTGCAACCGTTGTTATCTTCGGGACCGTCGCGATTTTCCTCTACCCgac                                           <  1:2051605/100‑3 (MQ=255)
                                                       ggCGACTGAGCCGGTAGTGAAAGCGGAAGCCAGTAAAGTAACCGAGGCTGTTGCAACCGTTGTTATCTTCGGGACCGTCGCGATTTTCCTCTACCCGGCg                                          <  1:1379938/100‑1 (MQ=255)
                                                         cGACTGAGCCGGTAGTGAAAGCGGAAGCCAGTAAAGTAACCGTGGCTGTTGCAACCGTTGTTATCTTCGGGACCGTCGCGATTTTCCTCTACCCGGCGat                                        <  1:45171/100‑1 (MQ=255)
                                                         cGACTGAGCCGGTAGTGAAAGCGGAAGCCAGTAAAGTAACCGAGGCTGTTGCAACCGTTGTTATCTTCGGGACCGTCGCGATTTTCCTCTACCCGGCGat                                        >  1:1216400/1‑100 (MQ=255)
                                                            cTGAGCCGGTAGTGAAAGCGGAAGCCAGTAAAGTAACCGTGGCTGTTGCAACCGTTGTTATCTTCGGGACCGTCGCGATTTTCCTCTACCCGGCGatata                                     <  1:2022617/100‑1 (MQ=255)
                                                            cTGAGCCGGTAGTGAAAGCGGAAGCCAGTAAAGTAACCGTGGCTGTTGCAACCGTTGTTATCTTCGGGACCGTCGCGATTTTCCTCTACCCGGCGatata                                     >  2:1760609/1‑100 (MQ=255)
                                                             tGAGCCGGTAGTGAAAGCGGAAGCCAGTAAAGTAACCGTGGCTGTTGCAACCGTTGTTATCTTCGGGACCGTCGCGATTTTCCTCTACCCGGCGatatat                                    >  2:396127/1‑100 (MQ=255)
                                                              gAGCCGGTAGTGAAAGCGGAAGCCAGTAAAGTAACCGAGGCTGTTGCAACCGTTGTTATCTTCGGGACCGTCGCGATTTTCCTCTACCCGGCGatatat                                    >  2:477810/1‑99 (MQ=255)
                                                               aGCCGGTAGTTAAAGCGGAAGCCAGTAAAGTAACCGTGGTTGTTGCAACCGTTGTTATCTTCGGGACCGTCGCGATTTTCCTCTACCCGGCGATATATcc                                  <  1:1879553/100‑1 (MQ=255)
                                                                gCCGGTAGTGAAAGCGGAAGCCAGTAAAGTAACCGTGGCTGTTGCAACCGTTGTTATCTTCGGGACCGTCGCGATTTTCCTCTACCCGGCGATATATCCg                                 <  1:1670618/100‑1 (MQ=255)
                                                                gCCGGTAGTGAAAGCGGAAGCCAGTAAAGTAACCGAGGCTGTTGCAACCGTTGTTATCTTCGGGACCGTCGCGATTTTCCTCTACCCGGCGATATATCCg                                 >  1:2012958/1‑100 (MQ=255)
                                                                 ccGGTAGTGAAAGCGGAAGCCAGTAAAGTAACCGTGGCTGTTGCAACCGTTGTTATCTTCGGGACCGTCGCGATTTTCCTCTACCCGGCGATATATc                                   >  2:1801940/1‑97 (MQ=255)
                                                                 ccGGTAGTGAAAGCGGAAGCCAGTAAAGTAACCGTGGCTGTTGCAACCGTTGTTATCTTCGGGACCGTCGCGATTTTCCTCTACCCGGCGATATATCCGc                                >  1:1289313/1‑100 (MQ=255)
                                                                 ccGGTAGTGAAAGCGGAAGCCAGTAAAGTAACCGTGGCTGTTGCAACCGTTGTTATCTTCGGGACCGTCGCGATTTTCCTCTACCCGGCGATATATCCGc                                <  2:2766749/100‑1 (MQ=255)
                                                                 ccGGTAGTGAAAGCGGAAGCCAGTAAAGTAACCGTGGCTGTTGCAACCGTTGTTATCTTCGGGACCGTCGCGATTTTCCTCTAACCGGCGATATATCTGc                                >  1:732804/1‑100 (MQ=255)
                                                                  cGGTAGTGAAAGCGGAAGCCAGTAAAGTAACCGTGGCTGTTGCAACCGTTGTTATCTTCGGGACCGTCGCGATTTTCCTCTACCCGGCGATATATCCGCt                               >  1:2227013/1‑100 (MQ=255)
                                                                  cGGTAGTGAAAGCGGAAGCCAGTAAAGTAACCGTGGCTGTTGCAACCGTTGTTATCTTCGGGACCGTCGCGATTTTCCTCTACCCGGCGATATATCCGCt                               <  1:765654/100‑1 (MQ=255)
                                                                   ggTCGTGAAAGCGGAAGCCAGTAAAGTAACCGTGGCTGTTGCAACCGTGGTTATCTTAGGGACCGTCGCGATTTTCCTCTGCTCGCCGATCTATCCGCTg                              >  2:1314989/1‑100 (MQ=255)
                                                                   ggTAGTGAAAGCGGAAGCCAGTAAAGTAACCGTGGCTGTTGCAACCGTTGTTATCTTCGGGACCGTCGCGATTTTCCTCTACCCGGCGATATATCCGCt                               <  1:710881/99‑1 (MQ=255)
                                                                   ggTAGTGAAAGCGGAAGCCAGTAAAGTAACCGTGGCTGTTGCAACCGTTGTTATCTTCGGGACCGTCGCGATTTTCCTCTACCCGGCGATATATCCGCTg                              >  1:475060/1‑100 (MQ=255)
                                                                   ggTAGTGAAAGCGGAAGCCAGTAAAGTAACCGAGGCTGTTGCAACCGTTGTTATCTTCGGGACCGTCGCGATTTTCCTCTACCCGGCGATATATCCGCTg                              >  1:2984341/1‑100 (MQ=255)
                                                                     tAGTGAAAGCGGAAGCCAGTAAAGTAACCGTGGCTGTTGCAACCGTTGTTATCTTCGGGACCGTCGCGATTTTCCTCTACCCGGCGATATATCCGCTGAt                            <  2:2982468/100‑1 (MQ=255)
                                                                        tGAAAGCGGAAGCCAGTAAAGTAACCGAGGCTGTTGCAACCGTTGTTATCTTCGGGACCGTCGCGATTTTCCTCTACCCGGCGATATATCCGCTGATGtc                         >  1:1672666/1‑100 (MQ=255)
                                                                        tGAAAGCGGAAGCCAGTAAAGTAACCGAGGCTGTTGCAACCGTTGTTATCTTCGGGACCGTCGCGATTTTCCTCTACCCGGCGATATATCCGCTGATGtc                         >  1:2809736/1‑100 (MQ=255)
                                                                         gAAAGCGGAAGCCAGTAAAGTAACCGTGGCTGTTGCAACCGTTGTTATCTTCGGGACCGTCGCGATTTTCCTCTACCCGGCGATATATCCGCTGATGtct                        <  2:2093206/100‑1 (MQ=255)
                                                                         gAAAGCGGAAGCCAGTAAAGTAACCGAGGCTGTTGCAACCGTTGTTATCTTCGGGACCGTCGCGATTTTCCTCTACCCGGCGATATATCCGCTGATGtct                        >  2:37543/1‑100 (MQ=255)
                                                                         gAAAGCGGAAGCCAGTAAAGTAACCGAGGCTGTTGCAACCGTTGTTATCTTCGGGACCGTCGCGATTTTCCTCTACCCGGCGATATATCCGCTGATGtct                        >  1:2157166/1‑100 (MQ=255)
                                                                         gAAAGCGGAAGCCAGTAAAGTAACCGAGGCTGTTGCAACCGTTGTTATCTTCGGGACCGTCGCGATTTTCCTCTACCCGGCGATATATCCGCTGATGtct                        >  1:760092/1‑100 (MQ=255)
                                                                         aaaaGCGGAAGCCAGTAAAGTAACCGTGGCTGTTGCACACGTTGTTATATTAGGGAACGTCGCGATTTTCCTCTACCCGGCGATATATCCGCTGTTAtct                        >  2:1040639/2‑100 (MQ=255)
                                                                          aaaGCGGAAGCCAGTAAAGTAACCGTGGCTGTTGCAACCGTTGTTATCTTCGGGACCGTCGCGATTTTCCTCTACCCGGCGATATATCCGCTGATGtctc                       >  1:2487677/1‑100 (MQ=255)
                                                                             gCGGAAGCCAGTAAAGTAACCGTGGCTGTTGCAACCGTTGTTATCTTCGGGACCGTCGCGATTTTCCGCTACCCGGCGATATATCCGCTGATGTCTCa                      <  1:720247/98‑1 (MQ=255)
                                                                              cGGAAGCCAGTAAAGTAACCGAGGCTGTTGCAACCGTTGTTATCTTCGGGACCGTCGCGATTTTACTCTACACGGCGATATATCCGCTGATGTCTCAATg                   >  2:1672390/1‑100 (MQ=255)
                                                                               ggAAGCCAGTAAAGTAACCGTGGCTGTTGCAACCGTTGTTATCTTCGGGACCGTCGCGATTTTCCTCTACCCGGCGATATATCCGCTGATGTCTCAATgg                  <  1:860266/100‑1 (MQ=255)
                                                                               ggAAGCCAGTAAAGTAACCGAGGCTGTTGCAACCGTTGTTATCTTCGGGACCGTCGCGATTTTCCTCTACCCGGCGATATATCCGCTGATGTCTCAATgg                  >  1:2868473/1‑100 (MQ=255)
                                                                                  aGCCAGTAAATTAACCGTGGCTGTTGCAACCGTTGTTATCTTCGGGACCGTCGCGATTTTCCTCTACCCGGCGATATATCCGCTGATGTCTCAATGGttt               <  2:679166/100‑1 (MQ=255)
                                                                                     cAGTAAAGTAACCGTGGCTGTTGCAACCGTTGTTATCTTCGGGACCGTCGCGATTTTCCTCTACCCGGCGATATATCCGCTGATGTCTCAATGGTTTa              >  1:2966912/1‑98 (MQ=255)
                                                                                     cAGTAAAGTAACCGTGGCTGTTGCAACCGTTGTTATCTTCGGGACCGTCGCGATTTTCCTCTACCCGGCGATATATCCGCTGATGTCTCAATGGTTTAGt            >  1:1372412/1‑100 (MQ=255)
                                                                                      agtaaagtaaCCGTGGCTGTTGCAACCGTTGTTATCTTCGGGACCGTCGCGATTTTCCTCTACCCGGCGATATATCCGCTGATGTCTCAATGGTTTAGTc           <  2:2753454/100‑1 (MQ=255)
                                                                                      agtaaagtaaCCGTGGCTGTTGCAACCGTTGTTATCTTCGGGACCGTCGCGATTTTCCTCTACCCGGCGATATATCCGCTGATGTCTCAATGGTTTAGTc           <  2:1784394/100‑1 (MQ=255)
                                                                                      agtaaagtaaCCGTGGCTGTTGCAACCGTTGTTATCTTCGGGACCGTCGCGATTTTCCTCTACCCGCCGATATATCCGCTGATGTCTCAATGGTTTAGTc           >  2:944884/1‑100 (MQ=255)
                                                                                       gtaaagtaaCCGTGGCTGTTGCAACCGTTGTTATCTTCGGGACCGTCGCGATTTTCCTCTACCCGGCGATATATCCGCTGATGTCTCAATGGTTTAGTcc          <  1:2976888/100‑1 (MQ=255)
                                                                                       gtaaagtaaCCGTGGCTGTTGCAACCGTTGTTATCTTCGGGACCGTCGCGATTTTCCTCTACCCGGCGATATATCCGCTGATGTCTCAATGGTTTAGTcc          <  1:870411/100‑1 (MQ=255)
                                                                                       gtaaagtaaCCGTGCCTGTTGCAACCGTTGTTATCTTCGGGACCGTCGCGATTTTCCTCTACCCGGCGATATATCCGCTGATGTCTCAATGGTTTAGTcc          <  2:2065652/100‑1 (MQ=255)
                                                                                        taaagtaaCCGTGGCTGTTGCAACCGTTGTTATCTTCGGGACCGTCGCGATTTTCCTCTACCCGGCGATATATCCGCTGATGTCTCAATGGTTTAGTCCg         <  2:1049299/100‑1 (MQ=255)
                                                                                          aagtaaCCGTGGCTGTTGCAACCGTTGTTATCTTCGGGACCGTCGCGATTTTCCTCTACCCGGCGATATATCCGCTGATGTCTCAATGGTTTAGTCCGGa       <  1:1616557/100‑1 (MQ=255)
                                                                                           agtaaCCGTGGCTGTTGCAACCGTTGTTATCTTCGGGACCGTCGCGATTTTCCTCTACCCGGCGATATATCCGCTGATGTCTCAATGGTTTAGTCCGGaa      <  1:2472012/100‑1 (MQ=255)
                                                                                           agtaaCCGTGGCTGTTGCAACCGTTGTTATCTTCGGGACCGTCGCGATTTTCCTCTACCCGGCGATATATCCGCTGATGTCTCAATGGTTTAGTCCGGaa      <  1:2472037/100‑1 (MQ=255)
                                                                                           agtaaCCGTGGCTGTTGCAACCGTTGTTATCTTCGGGACCGTCGCGATTTTCCTCTACCCGGCGATATATCCGCTGATGTCTCAATGGTTTAGTCCGGaa      >  1:2090165/1‑100 (MQ=255)
                                                                                            gtaaCCGTGGCTGTTGCAACCGTTGTTATCTTCGGGACCGTCGCGATTTTCCTCTACCCGGCGATATATCCGCTGATGTCTCAATGGTTTAGTCCGGaaa     >  1:686550/1‑100 (MQ=255)
                                                                                              aaCCGAGGCTGTTGCAACCGTTGTTATCTTCGGGACCGTCGCGATTTTCCTCTACCCGGCGATATATCCGCTGATGTCTCAATGGTTTAGTCCGGAAACt   >  1:985748/1‑100 (MQ=255)
                                                                                               aCCGTGGCTGTTGCAACCGTTGTTATCTTCGGGACCGTCGCGATTTTCCTCTACCCGGCGACATATCCGCTGATGTCTCAATGGTTTAGTCCGGAAACtt  >  2:2191260/1‑100 (MQ=255)
                                                                                                   |                                                                                               
ACACCAGCTGGTTGATCGGTGCCGGTAGCAGTATCTGTGGTGCTGCCGCGGTACTGGCGACTGAGCCGGTAGTGAAAGCGGAAGCCAGTAAAGTAACCGTGGCTGTTGCAACCGTTGTTATCTTCGGGACCGTCGCGATTTTCCTCTACCCGGCGATATATCCGCTGATGTCTCAATGGTTTAGTCCGGAAACTT  >  NC_000913/2250111‑2250305

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: