Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,278,298 C→T 100% intergenic (‑61/+272) yejG ← / ← bcr uncharacterized protein/bicyclomycin/cysteine/sulfonamide efflux transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,278,2980CT96.9% 300.5 / 0.2 97intergenic (‑61/+272)yejG/bcruncharacterized protein/bicyclomycin/cysteine/sulfonamide efflux transporter
Reads supporting (aligned to +/- strand):  ref base C (0/3);  new base T (46/48);  total (46/51)
Fisher's exact test for biased strand distribution p-value = 2.44e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.10e-01

Only 100 of 104 total aligned reads displayed.

GCAGTCGATGGACGATTGAGAGTTGTAATGAAGTCACTTATTTTCCCCGGAAGCACATTCACTTCACAATTTGTTTACATCAATTTTAACACATCATCAACAATCCGTCTTTATACAGATCGTAAAGATTGATGCCTCGCCTGCGTGGCCTCTATGGCTCTGATTTAAGTAATATCTGCGGGCGTGGTCAC  >  NC_000913/2278201‑2278391
                                                                                                 |                                                                                             
ttccgatctGGACGATTGAGAGTTGTAATGAAGTCACTTATTTTCCCCGGAAGCACATTCACTTCACAATTTGTTTACATCAATTTTAACACATCATtaa                                                                                             <  2:108153/92‑1 (MQ=255)
 cAGTCGATGGACGATTGAGAGTTGTAATGAAGTCACTTATTTTCCCCGGAAGCACATTCACTTCACAATTTGTTTACATCAATTTTAACACATCATtaac                                                                                            >  2:438869/1‑100 (MQ=255)
 cAGTCGATGGACGATTGAGAGTTGTAATGAAGTCACTTATTTCCCCCGGAAGCACATTCACTTCACAATTTGTTTACATCAATTTTAACACATCATtaac                                                                                            <  2:2479137/100‑1 (MQ=255)
  aGTCGATGGACGATTGAGAGTTGTAATGAAGTTACTTATTTTCCCCGGAAGCACATTCACTTCACAATTTGTTTACATCAATTTTAACACATCATtaaca                                                                                           <  1:851731/100‑1 (MQ=255)
   gTCGATGGACGATTGAGAGTTGTAATGAAGTCACTTATTTTCCCCGGAAGCACATTCACTTCACAATTTGTTTACATCAATTTTAACACATCATtaacaa                                                                                          >  1:64072/1‑100 (MQ=255)
     cGATGGACGATTGAGAGTTGTAATGAAGTCACTTATTTTCCCCGGAAGCACATTCACTTCACAATTTGTTTACATCAATTTTAACACATCATTAACAATc                                                                                        <  1:1871252/100‑1 (MQ=255)
     cGATGGACGATTGAGAGTTGTAATGAAGTCACTTATTTTCCCCGGAAGCACATTCACTTCACAATTTGTTTACATCAATTTTAACACATCATTAACAATc                                                                                        >  2:174516/1‑100 (MQ=255)
     cGATGGACGATTGAGAGTTGTAATGAAGTCACTTATTTTCCCCGGAAGCACATTCACTTCACAATTTGTTTACATCAATTTTAACACATCATTAACAATc                                                                                        >  1:797260/1‑100 (MQ=255)
     cGATGGACGATTGAGAGTTGTAATGAAGTCACTTATTTTCCCCGGAAGCACATTCACTTCACAATTTGTTTACATCAATTTTAACACATCATTAACAATc                                                                                        >  1:3021393/1‑100 (MQ=255)
      gATGGACGATTGAGAGTTGTAATGAAGTCACTTATTTTCCCCGGAAGCACATTCACTTCACAATTTGTTTACATCAATTTTAACACATCATTAACAATcc                                                                                       <  1:818152/100‑1 (MQ=255)
        tGGAAGATTGAGAGTTGTAATGAAGTCACTTATTTTCCCCGGAAGCACATTCACTTCACAATTTGTTTACATCAATTTTAACACATCATTAACAATCCGt                                                                                     >  2:563050/1‑100 (MQ=255)
          gACGATTGAGAGTTGTAATGAAGTCACTTATTTTCCCCGGAAGCACATTCACTTCACAATTTGTTTACATCAATTTTAACACATCATTAACAATCCGTCt                                                                                   <  2:2723920/100‑1 (MQ=255)
           aCGATTGAGAGTTGTAATGAAGTCACTTCTTTTCCCCGGAAGTACATTCACTTCACAATTTGTTTGCATCAATTTTAACATTTCATTAACAAGCCGTCtt                                                                                  <  2:1103960/100‑1 (MQ=255)
             gATTGAGAGTTGTAATGAAGTCACTTATTTTCCCCGGAAGCACATTCACTTCACAATTTGTTTACATCAATTTTAACACATCATTAACAATCCGTCTTta                                                                                <  2:2939155/100‑1 (MQ=255)
             gATTGAGAGTTGTAATGAAGTCACTTATTTTCCCCGGAAGCACATTCACTTCACAATTTGTTTACATCAATTTTAACACATCATTAACAATCCGTCTTta                                                                                <  1:999171/100‑1 (MQ=255)
              aTTGAGAGTTGTAATGAAGTCACTTATTTTCCCCGGAAGTACATTCACTTCACAATTTGTTTACATCAATTTTAACACATCATTAACAATCCGTCTTtat                                                                               <  1:221697/100‑1 (MQ=255)
                tGAGAGTTGTAATGAAGTCACTTATTTTCCCCGGAAGCACATTCACTTCACAATTTGTTTACATCAATTTTAACACATCATTAACAATCCGTCTTTATAc                                                                             <  2:2280437/100‑1 (MQ=255)
                 gagaGTTGTAATGAAGTCACTTATTTTCCCCGGAAGCACATTCACTTCACAATTTGTTTACATCAATTTTAACACATCATTAACAATCCGTCTTTATACa                                                                            >  1:1643009/1‑100 (MQ=255)
                 gagaGTTGTAATGAAGTCACTTATTTTCCCCGGAAGCACATTCACTTCACAATTTGTTTACATCAATTTTAACACATCATTAACAATCCGTCTTTATACa                                                                            >  2:566820/1‑100 (MQ=255)
                 gagaGTTGTAATGAAGTCACTTATTTTCCCCGGAAGCACATTCACTTCACAATTTGTTTACATCAATTTTAACACATCATCAACAATCCGTCTTTATACa                                                                            <  1:866521/100‑1 (MQ=255)
                  agagTTGTAATGAAGTCACTTATTTTCCCCGGAAGCACATTCACTTCACAATTTGTTTACATCAATTTTAACACATCATTAACAATCCGTCTTTATACAg                                                                           >  2:485731/1‑100 (MQ=255)
                  agagTTGTAATGAAGTCACTTATTTTCCCCGGAAGCACATTCACTTCACAATTTGTTTACATCAATTTTAACACATCATTAACAATCCGTCTTTATACAg                                                                           <  2:2862777/100‑1 (MQ=255)
                     gTTGTAATGAAGTCACTTATTTTCCCCGGAAGCACATTCACTTCACAATTTGTTTACATCAATTTTAACACATCATTAACAATCCGTCTTTATACAGATc                                                                        >  1:1640032/1‑100 (MQ=255)
                      ttGTAATGAAGTCACTTATTTTCCCCGGAAGCACATTCACTTCACAATTTGTTTACATCAATTTTAACACATCATTAACAATCCGTCTTTATACAGATCg                                                                       <  1:344273/100‑1 (MQ=255)
                       tttAATGATGTCACTTATTGTCCCCAGAAGCACATTCACTTCACAATTTGTTTACATCAATTTTAACACATCATTAACAATCCGTCTTTATACAGATCg                                                                       <  1:555083/97‑1 (MQ=255)
                       tGTAATGAAGTTACTTATTTTCCCCGGAAGCACATTTACTTCACAATTTGTTTACATCAATTTTAACACATCATTAACAATCCGTCTTTATACAGATCg                                                                       <  1:1766753/99‑1 (MQ=255)
                        gTAATGAAGTCACTTATTTTCCCCGGAAGCACATTCACTTCACAATTTGTTTACATCAATTTTAACACATCATTAACAATCCGTCTTTATACAGATCGTa                                                                     >  2:2613709/1‑100 (MQ=255)
                         tAATGAAGTCACTTATTTTCCCCGGAAGCACATTCACTTCACAATTTGTTTACATCAATTTTAACACATCATTAACAATCCGTCTTTATACAGATCGTaa                                                                    >  1:1543104/1‑100 (MQ=255)
                         tAATGAAGTCACTTATTTTCCCCGGAAGCACATTCACTTCACAATTTGTTTACATCAATTTTAACACATCATTAACAATCAGTCTTTATACAGATCGTaa                                                                    >  1:2538854/1‑100 (MQ=255)
                             gAAGTCACTTATTTTCCCCGGAAGCACATTCACTTCACAATTTGTTTACATCAATTTTAACACATCATTAACAATCCGTCTTTATACAGATCGTAAAGAt                                                                >  1:871809/1‑100 (MQ=255)
                              aaatCACTTATTTTCCCCGGAAGCACATTCACTTCACAATTTGTTTACATCAATTTTAACACATCATTAACAATCCGTCTTTATACAGATCGTAAAGAtt                                                               <  2:2957756/97‑1 (MQ=255)
                                gTCACTTATTTTCCCCGGAAGTACATTCACTTCACAATTTGTTTACATCAATTTTAACACATCATTAACAATCCGTCTTTATACAGATCGTAAAGATTGa                                                             <  1:586598/100‑1 (MQ=255)
                                 ttaCTTTTTTTCCCCTGAAGCGCATTCACTTCACAATTTGTTTACATCAATTTTAATACATCATTAACAATCCGTCTTTATACAGATCGTAAAGAtt                                                               <  2:237364/95‑1 (MQ=255)
                                 tCACTTATTTTCCCCGGAAGCACATTCACTTCACAATTTGTTTACATCAATTTTAACACATCATTAACAATCCGTCTTTATACAGATCGTAAAGATTGa                                                             <  1:216327/99‑1 (MQ=255)
                                  cACTTATTTTCCCCGTAAGCACATTCACTTCACAATTTGTTTACATCAATTTTAACACATCATTAACAATCCGTCTTTATACAGATCGTAAAGATTGATg                                                           <  1:1232937/100‑1 (MQ=255)
                                   aCTTATTTTCCCCGGAAGCACATTCACTTCTCAATTTGTTTACATCAATTTTAACACATCATTAACAATCCGTCTTTATACAGATCGTAAAGATTGATGc                                                          <  2:709915/100‑1 (MQ=255)
                                   aCTTATTTTCCCCGGAAGCACATTCACTTCAAAATTTGTTTACATCAATTTTAACACATCATTAACAATCCGTCTTTATACAGATCGTAAAGATTGAtaa                                                          >  2:2722912/1‑98 (MQ=255)
                                    cTTATTTTCCCCGGAAGCACATTCACTTCACAATTTGTTTACATCAATTTTAACACATCATTAACAATCCGTCTTTATACAGATCGTAAAGATTGATGcc                                                         >  2:2434089/1‑100 (MQ=255)
                                      tATTTTCCCCGGAAGCACATTCACTTCACAATTTGTTTACATCAATTTTAACACATCATTAACAATCCGTCTTTATACAGATCGTAAAGATTGATGCCTc                                                       >  2:472905/1‑100 (MQ=255)
                                      tATTTTCCCCGGAAGCACATTCACTTCACAATTTGTTTACATCAATTTTAACACATCATTAACAATCCGTCTTTATACAGATCGTAAAGATTGATGCCTc                                                       >  1:1617234/1‑100 (MQ=255)
                                       aTTTTCCCCGGAAGCACATTCACTTCACAATTTGTTTACATCAATTTTAACACATCATCAACAATCCGTCTTTATACAGATCGTAAAGATTGATGCCTCg                                                      <  2:345884/100‑1 (MQ=255)
                                       aTTTTCCCCGGAACCACATTCACTACACAATTTGTTTACATCAATTTTAACACATCATTAACAATCCGTCTTTATACAGATCGTAAAGATTGATGCCTCg                                                      >  2:1023405/1‑100 (MQ=255)
                                         tttCCGCGGAAGCACATTCACTTCACAATTTGCTTACATCAATTTTAACACATTATTAACAATCTGTCTTTATACAGTTCGTAAAGATTGATGCCTc                                                       <  2:1206645/97‑1 (MQ=255)
                                         tttCCCCTGAAGCACATTCACTTCAAAATTTGTTTACATCAATTTTAACACATCATTAACAATCCGTCTTTATACAGATCGTAAAGATTGATGCCTCGcc                                                    <  2:642287/100‑1 (MQ=255)
                                         tttCCCCGGAAGCACATTCACTTCACAATTTGTTTACATCAATTTTAACACATCATTAACAATCCGTCTTTATACAGATCGTAAAGATTGATGCCTCGcc                                                    <  2:1462535/100‑1 (MQ=255)
                                          ttCCCCGGAAGCACATTCACTTCACAATTTGTTTACATCAATTTTAACACATCATTAACAATCCGTCTTTATACAGATCGTAAAGATTGATGCCTCGCCt                                                   >  1:801166/1‑100 (MQ=255)
                                           tCCCCGGAAGCACATTCACTTCACAATTTGTTTACATCAATTTTAACACATCATTAACAATCCGTCTTTATACAGATCGTAAAGATTGATGCCTCGCCta                                                  <  1:2125798/100‑2 (MQ=255)
                                             cccGGAAGCACATTCACTTCACAATTTGTTTACATCAATTTTAACACATCATTAACAATCCGTCTTTATACAGATCGTAAAGATTGATGcagatcggaag                                                >  1:1969948/1‑90 (MQ=255)
                                               cGGAAGCACATTCACTTCACAATTTGTTTACATCAATTTTAACACATCATTAACAATCCGTCTTTATACAGATCGTAAAGATTGATGCCTCGCCTGCGTg                                              <  1:1947221/100‑1 (MQ=255)
                                               cGGAAGCACATTCACTTCACAATTTGTTTACATCAATTTTAACACATCATTAACAATCCGTCTTTATACAGATCGTAAAGATTGATGCCTCGCCTGCGTg                                              <  2:1594542/100‑1 (MQ=255)
                                                ggAAGCACATTCACTTCACAATTTGTTTACATCAATTTTAACACATCATTAACAATCCGTCTTTATACAGATCGTAAAGATTGATGCCTCGCCTGCGTgg                                             <  1:731476/100‑1 (MQ=255)
                                                 gAAGCACATTCACTTCACAATTTGTTTACATCAATTTTAACACATCATTAACAAGCCGTCTTTATACAGATCGTAAAGATTGATGCCTCGCCTGCGTgg                                             <  2:2708284/99‑1 (MQ=255)
                                                 gAAGCACATTCACTTCACAATTTGTTAACATCAATTTTAACACATCATTAACAATCTGACTTTATACAGATCATCAAGATTGATGCCTCGCCTGCGTg                                              >  2:465800/1‑98 (MQ=255)
                                                  aaGCACATTCACTTCACAATTTGTTTACATCAATTTTAACACATCATTAACAATCCGTCTTTATACAGATCGTAAAGATTGATGCCTCGCCTGCGTGGcc                                           <  1:7735/100‑1 (MQ=255)
                                                   aGCACATTCCCTTCACAATTTGTTTACATCAATTTTAACACATCATTAACAATCCGTCTTTATACAAATCGTAAAGATTAAAGCCTCGCCTGCGTgg                                             >  2:1114186/1‑97 (MQ=255)
                                                    gCACATTCACTTCACAATTTGTTTACATCAATTTTAACACATCATTAACAATCCGTCTTTATACAGATCGTAAAGATTGATGCCTCGCCTGCGTGGCctc                                         <  1:1329882/100‑1 (MQ=255)
                                                    gCACATTCACTTCACAATTTGTTTACATCAATTTTAACACATCATTAACAATCCGTCTTTATACAGATCGTAAAGATTGATGCCTCGCCTGCGTGGCctc                                         <  1:163568/100‑1 (MQ=255)
                                                      acaTTCACTTCACAATTTGTTTACATCAATTTTAACACATCATTAACAATCCGTCTTTATACAGATCGTAAAGATTGATGCCTCGCCTGCGTGGCCTCTa                                       >  2:2668220/1‑100 (MQ=255)
                                                       caTTCACTTCACAATTTGTTTACATCAATTTTAACACATCATTAACAATCCGTCTTTATACAGATCGTAAAGATTGATGCCTCGCCTGCGTGGCCTCTAt                                      >  2:2397801/1‑100 (MQ=255)
                                                       caTTCACTTCACAATTTGTTTACATCAATTTTAACACATCATTAACAATCCGTCTTTATACAGATCGTAAAGATTGATGCCTCGCCTGCGTGGCCTCTAt                                      >  1:279212/1‑100 (MQ=255)
                                                        aTTCACTTCACAATTTGTTTACATCAATTTTAACACATCATTAACAATTCGTCTTTATACAGATCGTAAAGATTGATGCCTCGCCTGCGTGGCCTCTATg                                     <  1:783532/100‑1 (MQ=255)
                                                          tcacttcacAATTTGTTTACATCAATTTTAACACATCATTAACAATCCGTCTTTATACAGATCGTAAAGATTGATGCCTCGCCTGCGTGGCCTCTATGGc                                   <  1:2594476/100‑1 (MQ=255)
                                                          tcacttcacAATTTGTTTACATCAATTTTAACACATCATTAACAATCCGTCTTTATACAGATCGTAAAGATTGATGCCTCGCCTGCGTGGCCTCTATGGc                                   <  2:1736458/100‑1 (MQ=255)
                                                            gcttcacACTTTGTTTACATCAATTATAACACATCATTCACAATCCGTCTTTATACAGGTCGTCAAGATTGATGCATCACCTGCTTGGCCTCTATGGc                                   >  2:2910109/2‑98 (MQ=255)
                                                              ttcacAATTTGTTTACATCAATTTTAACACATCATTAACAATCCGTCTTTATACAGATCGTAAAGATTGATGCCTCGCCTGCGTGGCCTCTATGGCTCTg                               <  1:2598601/100‑1 (MQ=255)
                                                                cacaATTTGTTTACATCAATTTTAACACATCATCAACAATCCGTCTTTATACAGATCGTAAAGATTGATGCCTCGCCTGCGTGGCCTCTATGGCTCTGAt                             <  2:262806/100‑1 (MQ=255)
                                                                 acaATTTGTTTACATCAATTTTAACACATCATTAACAATCCGTCTTTATACAGATCCTAAAGATTGATGCCTCGCCTGCGTGGCCTCTATGGCCCTGAt                             >  1:1549404/1‑99 (MQ=255)
                                                                   aaTTTGTTTACATCAATTTTAACACATCATTAACAATCCGTCTTTATACAGATCGTAAAGATTGATGCCTCGCCTGCGTGGCCTCTATGGCTCTGATTTa                          >  1:1570468/1‑100 (MQ=255)
                                                                     tttGTTTACATCAATTTTAACACATTATTAACAATCCGTCTTTATACAGATCGTAAAGATTGATGCCTCGCCTGCGTGGCCTCTATGGCTCTGATTTAAg                        <  2:2022902/100‑1 (MQ=255)
                                                                     tttGTTTACATCAATTTTAACACATCATTAACAATCCGTCTTTATACAGATCGTAAAGATTGATGCCTCGCCTGCGTGGCCTCTATGGCTCTGATTTAAg                        <  2:801162/100‑1 (MQ=255)
                                                                      ttGTTTACATCAATTTTAACACATCATTAACAATCCGTCTTTATACAGATCGTAAAGATTGATGCCTCGCCTGCGTGGCCTCTATGGCTCTGATTTAAGt                       <  1:1975342/100‑1 (MQ=255)
                                                                       tGTTTACATCAATTTTAACACATCATTAACAATCCGTCTTTATACAGATCGTAAAGATTGATGCCTCGCCTGCGTGGCCTCTATGGCTCTGATTTAAGTa                      <  1:726285/100‑1 (MQ=255)
                                                                           tACATCAATTTTAACACATCATTAACAATCCGTCTTTATACAGATCGTAAAGATTGATGCCTCGCCTGCGTGGCCTCTATGGCTCTGATTTAAGTAatat                  >  2:1536001/1‑100 (MQ=255)
                                                                            aCATCAATTTTAACACATCATTAACAATCCGTCTTTATACAGATCGTAAAGATTGATGCCTCGCCTGCGTGGCCTCTATGGCTCTGATTTAAGTAATATc                 <  2:2931744/100‑1 (MQ=255)
                                                                             cATCAATTTTAACACATCATTAACAATCCGTCTTTATACAGATCGTAAAGATTGATGCCTCGCCTGCGTGGCCTCTATGGCTCTGATTTAAGTAATATCt                >  1:1014897/1‑100 (MQ=255)
                                                                             cATCAATTTTAACACATCATTAACAATCCGTCTTTATACAGATCGTAAAGATTGATGCCTCGCCTGCGTGGCCTCTATGGCTCTGATTTAAGTAATATCt                >  1:2401156/1‑100 (MQ=255)
                                                                             cATCAATTTTAACACATCATTAACAATCCGTCTTTATACAGATCGTAAAGATTGATGCCTCGACTGCGTGGCTTCTATGGCTCTGATTTAAGTAATATCt                >  2:565781/1‑100 (MQ=255)
                                                                             cATCAATTTTAACACATCATTAACAATCCGTCTTTATACAGATCGTAAAGATTGATACCTCGCCTGAGTAGCCTATATGTCTCTGATATAAGTAatatat                >  1:477870/1‑98 (MQ=255)
                                                                              aTCAATTTTAACACATCATTAACAATCCGTCTTTATACAGATCGTAAAGATTGATGCCTCGCCTGCGTGGCCTCTATGGCTCTGATTTAAGTAATATCTg               >  1:1540762/1‑100 (MQ=255)
                                                                               tCAATTTTAACACATCATTAACAATCCGTCTTTATACAGATCGTAAAGATTCATGCCTCGCCTGCGTGGCCTCTATGACACAGAACAAAATAATATCt                >  1:745000/1‑98 (MQ=255)
                                                                                cAATTTTAACACATCATTATCAATCCGTCTTTATACAGATCGTAAAGATTGATGCCTCGCCTGCGTGGCCTCTATGGCTCTGATTTAAGTAATATCTGCg             <  2:220420/100‑1 (MQ=255)
                                                                                cAATTTTAACACATCATTAACAATCCGTCTTTATACAGATCGTAAAGATTGATGCCTCGCCTGCGTGGCCTCTATGGCTCTGATTTAAGTAATATCTGCg             <  1:933023/100‑1 (MQ=255)
                                                                                 aaTTTTAACACATCATTAACAATCCGTTTTTATACAGATCGTAAAGATTGATGCCTCGCCTGCGTGGCCTCTATGGCTCTGATTTAAGTAATATCTGCgg            <  1:1812820/100‑1 (MQ=255)
                                                                                 aaTTTTAACACATCATTAACAATCCGTCTTTATACAGATCGTAAAGATTGATGCCTCGCCTGCGTGGCCTCTATGGCTCTGATTTAAGTAATATCTGCgg            <  1:1952386/100‑1 (MQ=255)
                                                                                 aaTTTTAACACATCATTAACAATCCGTCTTTATACAGATCGTAAAGATTGATGCCTCACCTGCGTGGCCTCTATGGCTCTGATTTAAGTAATATCTGCgg            >  2:2136987/1‑100 (MQ=255)
                                                                                  aTTTTAACACATCATTAACAATCCGTCTTTATACAGATCGTAAAGATTGATGCCTCGCCTGCGTGGCCTCTATGGCTCTGATTTAAGTAATATCTGCggg           <  2:1243139/100‑1 (MQ=255)
                                                                                   ttttAACACATCATTAACAATCCGTCTTTATACAGTTCGTAAAGATTGATGCCTCGCCTGCGTGGCCTCTATGGCTCTGATTTAAGTAATATCTGCGGGc          <  1:2325629/100‑1 (MQ=255)
                                                                                     ttAACACATCATTGACAATCCGTTTTTATACAGATTGTAAAGATTGATGCCTCGCCTGCGTGGCCTCTATGGCTCTGATTTAAGTAATATCTGCGGGCGt        <  1:474193/100‑1 (MQ=255)
                                                                                     ttAACACATCATTAACAATCCGTCTTTATACAGCTCGTAAAGATTGATGCCTCGCCTGCGTGGCCTCTATGGCTCTGATTTAAGTAATATCTGCGGGCGt        >  2:1813730/1‑100 (MQ=255)
                                                                                     ttAACACATCATTAACAATCCGTCTTTATACAGATCGTAAAGATTGATGCCTCGCCTGCGTGGCCTCTATGGCTCTGATTTAAGTAATATCTGCGGGCGt        <  1:1536019/100‑1 (MQ=255)
                                                                                       aaCACATCATTAACAATCCGTCTTTATACAGATCGTAAAGATTGATGCATCGCCTGCGTGGCCTCTATGGCTCTGATTTAAGTAATATCTGCGGGCGTgg      >  1:20838/1‑100 (MQ=255)
                                                                                        acacATCATTAACAATCCGTCTTTATACAGATCGTAAAGATTGATGCCTCGCCTGCGTGGCCTCTATGGCTCTGATTTAAGTAATATCTGCGGGCGTGGt     >  2:476180/1‑100 (MQ=255)
                                                                                         cacaTCCTTACCAATCCGTCTTTATACAGATCGTAAAGATTGATGCCTCGCCTGAGTGGCCTCTATCGCGCTGATTTAAGTAATATCTGCAGGCGt        >  2:2682987/1‑96 (MQ=255)
                                                                                         cacaTCATTAACAATCCGTCTTTATACAGATCGTAAAGATTGATGCCTCGCCTGCGTGGCCTCTATGGCTCTGATTTAAGTAATATCTGCGGGCGTgg      >  2:659049/1‑98 (MQ=255)
                                                                                         cacaTCATTAACAATCCGTCTTTATACAGATCGTAAAGATTGATGCCTCGCCTGCGTGGCCTCTATGGCTCTGATTTAAGTAATATCTGCGGGCGTGGTc    <  1:1561677/100‑1 (MQ=255)
                                                                                         cacaTCATTAACAATCCGTCTTTATACAGATCGTAAAGATTGATGCCTAGCCTGCGTGGCCTCAATGGCTCTGATTTAAGAAATATCTGCGGGCGTGGTc    >  1:918713/1‑100 (MQ=255)
                                                                                          acaTCATTAACAATCCGTCTTTATACAGATCGTAAAGATTGATGCCTCGCCTGCGTGGCCTCTATGGCTCTGATTTAAGTAATATCTGCGGGCGTGGTCa   >  1:977412/1‑100 (MQ=255)
                                                                                          acaTCATTAACAATCCGTCTTTATACAGATCGTAAAGATTGATGCCTCGCCTGCGTGGCCTCTATGGCTCTGATTTAAGTAATATCTGCGGGCGTGGTCa   >  2:2118527/1‑100 (MQ=255)
                                                                                           catcatTAACAATCCGTCTTTATACAGATCGTAAAGATTGATGCCTCGCCTGCGTGGCCTCTATGGCTCTGATTTAAGTAATATCTGCGGGCGTGGTCAc  >  1:1341594/1‑100 (MQ=255)
                                                                                              cattaACAGTCCGTATTTATACAGATCGTAAAGATTGATGCCTCGCCTGCGTGGCCTCTATGGCTCTGATTTAAGTAATATCTGCGGGCGTGGTCAc  <  2:2271215/97‑1 (MQ=255)
                                                                                                 |                                                                                             
GCAGTCGATGGACGATTGAGAGTTGTAATGAAGTCACTTATTTTCCCCGGAAGCACATTCACTTCACAATTTGTTTACATCAATTTTAACACATCATCAACAATCCGTCTTTATACAGATCGTAAAGATTGATGCCTCGCCTGCGTGGCCTCTATGGCTCTGATTTAAGTAATATCTGCGGGCGTGGTCAC  >  NC_000913/2278201‑2278391

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: