Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,307,386 A→T 100% D410E (GAT→GAA yojI ← microcin J25 efflux ABC transporter permease/ATPase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,307,3860AT97.8% 367.7 / ‑1.6 114D410E (GAT→GAAyojImicrocin J25 efflux ABC transporter permease/ATPase
Reads supporting (aligned to +/- strand):  ref base A (1/2);  new base T (55/56);  total (56/58)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.77e-01

Only 100 of 114 total aligned reads displayed.

CGTTGCTTAACTCAAGCTTATGAGCCATTTTCAGCTGCGCCAGCCACTTCTCAACCAGTTGCGGGTTAGCGGGTTTACCCTCCGGCCCCAGCAGTTGATCAAACAGCCAGACATCGGTAAACACTGCCGAAAACAGTTTGCGATAATCTTCCGGTTGTTCGCCGCTGACAGGTTTGCCATCCAGCAAGATTTCG  >  NC_000913/2307289‑2307482
                                                                                                 |                                                                                                
cGTTGCTTAACTCAAGCTTATGAGCCATTTTCAGCTGCGCCAGCCACTTCTCAACCAGTTGCGGGTTAGCGGGTTTACCCTCCGGCCCCAGCAGTTGTTc                                                                                                <  1:2374661/100‑1 (MQ=255)
 gTTGCTTAACTCAAGCTTATGAGCCATTTTCAGCTGCGCCAGCCACTTCTCAACCAGTTGCGGGTTAGCGGGTTTACCCTCCGGCCCCAGCAGTTGTTCa                                                                                               <  1:241371/100‑1 (MQ=255)
 gTTGCTTAACTCAAGCTTATGAGCCATTTTCAGCTGCGCCAGCCACTTCTCAACCAGTTGCGGGTTAGCGGGTTTACCCTCCGGCCCCAGCAGTTGTTCa                                                                                               >  1:2676937/1‑100 (MQ=255)
  ttGCTTAACTCAAGCTTATGAGCCATTTTCAGCTGCGCCAGCCACTTCTCAACCAGTTGCGGGTTAGCGGGTTTACCCTCCGGCCCCAGCAGTTGTTCaa                                                                                              >  1:2622725/1‑100 (MQ=255)
  ttGCTTAACTCAAGCTTATGAGCCATTTTCAGCTGCGCCAGCCACTTCTCAACCAGTTGCGGGTTAGCGGGTTTACCCTCCGGCCCCAGCAGTTGTTCaa                                                                                              <  1:2319573/100‑1 (MQ=255)
     cTTAACTCAAGCTTATGAGCCATTTTCAGCTGCGCCAGCCACTTCTCAACCAGTTGCGGGTTAGCGGGTTTACCCTCCGGCCCCAGCAGTTGTTCAAACa                                                                                           >  1:1645092/1‑100 (MQ=255)
     cTTAACTCAAGCTTATGAGCCATTTTCAGCTGCGCCAGCCACTTCTCAACCAGTTGCGGGTTAGCGGGTTTACCCTCCGGCCCCAGCAGTTGTTCAAACa                                                                                           >  1:2849840/1‑100 (MQ=255)
     cTTAACTCAAGCTTATGAGCCATTTTCAGCTGCGCCAGCCACTTCTCAACCAGTTGCGGGTTAGCGGGTTTACCCTCCGACCCCAGCAGTTGTTCAAACa                                                                                           >  1:2713169/1‑100 (MQ=255)
     cTTAACTCAAGCTTATGAGCCATTTTCAGCTGCACCAGCCACTTCTCAATCAGTTGCGGGTTAGCGGGTTGACCCTCCGGCCCCAACAGTTGATCGAACa                                                                                           >  2:879574/1‑100 (MQ=255)
       tAACTCAAGCTTATGAGCCATTTTCAGCTGCGCCAGCCACTTCTCAACCAGTTGCGGGTTAGCGGGTTTACCCTCCGGCCCCAGCAGTTGTTCAAACAGc                                                                                         >  2:228799/1‑100 (MQ=255)
        aaCTCAAGCTTATGAGCCATTTTCAGCTGCGCCAGCCACTTCTCAACCAGTTGCGGGTTAGCGGGTTTACCCTCCGGCCCCAGCAGTTGTTCAAACAGcc                                                                                        <  2:365582/100‑1 (MQ=255)
        aaCTCAAGCTTATGAGCCATTTTCAGCTGCGCCAGCCACTTCTCAACCAGTTGCGGGTTAGCGGGTTTACCCTCCGGCCCCAGCAGTTGTTCAAACAGcc                                                                                        <  1:1710845/100‑1 (MQ=255)
         aCTCAAGCTTATGAGCCATTTTCAGCTGCACCAGCCACTTCTCAATCAGTTGCGGGTTAGCGGGTTGACCCTCCGGCCCCAACAGTTGATCGAACAGCCa                                                                                       <  1:879580/100‑1 (MQ=255)
          cTCAAGCTTATGAGCCATTTTCAGCTGCGCCAGCCACTTCTCAACCAGTTGCGGGTTAGCGGGTTTACCCTCCGGCCCCAGCAGTTGTTCAAACAgatcg                                                                                      >  2:2364494/1‑96 (MQ=255)
          cTCAAGCTTATGAGCCATTTTCAGCTGCGCCAGCCACTTCTCAACCAGTTGCGGGTTAGCGGGTTTACCCTCCGGCCCCAGCAGTTGTTCAAACAGCCAg                                                                                      <  1:651011/100‑1 (MQ=255)
          cTCAAGCTTATGAGCCATTTTCAGCTGCGCCAGCCACTTCTCAACCAGTTGCGGGTTAGCGGGTTTACCCTCCGGCCCCAGCAGTTGTTCAAACAGCCAg                                                                                      >  1:2596861/1‑100 (MQ=255)
          cTCAAGCTTATGAGCCATTTTCAGCTGCGCCAGCCACTTCTCAACCAGTTGCGGGTTAGCGGGTTTACCCTCCGGCCCCAGCAGTTGTTCAAACAGCCAg                                                                                      >  1:2407806/1‑100 (MQ=255)
           tCAAGCTTATGAGCCATTTTCAGCTGCGCCAGCCACTTCTCAACCAGTTGCGGGTTAGCGGGTTTACCCTCCGGCCCCAGCAGTTGTTCAAACAGCCan                                                                                      <  2:2964472/99‑2 (MQ=255)
           tCAAGCTTATGAGCCATTTTCAGCTGCGCCAGCCACTTCTCAACCAGTTGCGGGTTAGCGGGTTTACCCTCCGGCCCCAGCAGTTGTTCAAACAGCCAGa                                                                                     <  1:294073/100‑1 (MQ=255)
           tCAAGCTTATGAGCCATTTTCAGCTGCGCCAGCCACTTCTCAACCAGTTGCGGGTTAGCGGGTTTACCCTCCGGCCCCAGCAGTTGTTCAAACAGCCAGa                                                                                     >  1:380325/1‑100 (MQ=255)
            cAAGCTTATGAGCCATTTTCAGCTGCGCCAGCCACTTCTCAACCCGTTGCGTGTTAGCGGCTTAATCCTCCAGCCAAAGCAATTGTTTAAACAGCCAg                                                                                      >  1:115596/1‑98 (MQ=255)
            cAAGCTTATGAGCCATTTTCAGCTGCGCCAGCCACTACTCAACCAGTTGCGGGTTAGCGGGTTTACCCTCCGGCCCCAGCAGTTGTTCAAACAGCCAGAc                                                                                    >  1:2138520/1‑100 (MQ=255)
              accTTATGATCCATTTTCAGCTGCGCCAGCCACTTCTCAACCAGTTGCGGGTTATCGGTTTTACCCTCCGGCTCCATCAGTTTTTCAAACAGCCAGACa                                                                                   <  2:616362/97‑1 (MQ=255)
                cTTATGAGCCATTTTCAGCTGCGCCAGCCACTTCTCAACCAGTTGCGGGTTAGCGGGTTTACCCTCCGGCCCCAGCAGTTGTTCAAACAGCCAGACATc                                                                                 <  1:602197/99‑1 (MQ=255)
                 ttATGAGCCATTTTCAGCTGCGCCAGCCACTTCTCAACCAGTTGCGGGTTAGCGGGTTTACCCTCCGGCCCCAGCAGTTGTTCAAACAGCCAGACATCgg                                                                               <  1:1383211/100‑1 (MQ=255)
                  naTGAGCCATTTTCAGCTGCGCCAGCCACTTCTCAACCAGTTGCGGGTTAGCGGGTTTACCCTCCGGCACCAGCAGTTGTTCAAACAGCCAGACATCgg                                                                               >  2:948137/2‑99 (MQ=255)
                   aTGAGCCATTTTCAGCTGCGCCAGCCACTTCTCAACCAGTTGCGGGTTAGCGGGTTTACCCTCCGGCACCAGCAGTTGTTCAAACAGCCAGACATCGGTa                                                                             >  1:1204164/1‑100 (MQ=255)
                    tGAGCCATTTTCAGCTGCGCCAGCCACTTCTCAACCAGTTGCGGGTTAGCGGGTTTACCCTCCGGCCCCAGCAGTTGTTCAAACAGCCAGACATCGGTaa                                                                            <  2:1197752/100‑1 (MQ=255)
                     gAGCCATTTTCAGCTGCGCCAGCCACTTCTCAACCAGTTGCGGGTTAGCGGGTTTACCCTCCGGCCCCAGCAGTTGTTCAAACAGCCAGACATCGGTaaa                                                                           >  2:3010949/1‑100 (MQ=255)
                     gAGCCATTTTCAGCTGCGCCAGCCACTTCTCAACCAGTTGCGGGTTAGCGGGTTTACCCTCCGGCCCCAGCAATTGTTCAAACAGCCAGACATCGGTaaa                                                                           >  1:1293444/1‑100 (MQ=255)
                     gAGCCACCTTCAGCTGCGCCAGCCACTACTTAACTAGTGGCGGGTTAGCGGCTTTAGCCTGCGCCTTTAGCAGTTGTTCTAACAGCCAGACATCGGTaaa                                                                           >  2:1938184/1‑100 (MQ=255)
                      aGCCATTTTCAGCTGCGTCAGCCATTTTTTTACCAGTTGCGGGTTAGCGTGTTTACTCTTCGGACCCAGCAGTTGTTCAAACAGCCAGACATCGGTaaa                                                                           <  1:22227/99‑1 (MQ=255)
                       gCCATTTTCAGCTGCGCCAGCCACTTCTCAACCAGTTGCGGGTTAGCGGGTTTACCCTCCGGCCCCAGCAGTTGTTCAAACAGCCAGACATCGGTAAaca                                                                         <  1:1207600/100‑1 (MQ=255)
                       gCCATTTTCAGCTGCGCCAGCCACTTCTCAACCAGTTGCGGGTTAGCGGGTTTACCCTCCGGCCCCAGCAGTTGTTCAAACAGCCAGACATCGGTAAaca                                                                         <  2:584281/100‑1 (MQ=255)
                       gCCATTTTCAGCTGCGCCAGCCACTTCTCAACCAGTTGCGGGTTAGCGGGTTTACCCTCCGGCCCCAGCAGTTGTTCAAACAGCCAGACATCGGTAAaca                                                                         >  2:905170/1‑100 (MQ=255)
                         cATTTTCAGCTGCGCCAGCCACTTCTCAACCAGTTGCGGGTTAGCGGTTTTACCATCCGGCCCCAGCAGTTGTTCAAACAGCCAGACATCGGTAAACACt                                                                       >  2:1065515/1‑100 (MQ=255)
                         cATTTTCAGCTGCGCCAGCCACTTCTCAACCAGTTGCGGGTTAGCGGGTTTACCCTCCGGCCCCAGCAGTTGTTCAAACAGCCAGACATCGGTAAACACt                                                                       >  1:2463930/1‑100 (MQ=255)
                         cATTTTCAGCTGCGCCAGCCACTTCTCAACCAGTTGCGGGTTAGCGGGTTTACCCTCCGGCCCCAGCAGTTGTTCAAACAGCCAGACATCGGTAAACACt                                                                       >  1:52344/1‑100 (MQ=255)
                         cATTTTCAGCTGCGCCAGCCACTTCTCAACCAGTTGCGGGTTAGCGGGTTTACCCTCCGGCCCCAGCAGTTGTTCAAACAGCCAGACATCGGTAAACACt                                                                       <  1:2761461/100‑1 (MQ=255)
                            tttCAGCTGCGCCAGCCACTTCTCAACCAGTTGCGGGTTAGCGGGTTTACCCTCCGGCCCCAGCAGTTGTTCAAACAGCCAGACATCGGTAAACACTGcc                                                                    <  1:1208167/100‑1 (MQ=255)
                             ttCAGCTGCGCCAGCCACTTCTCAACCAGTTGCGGGTTAGCGGGTTTACCCTCCGGCCCCAGCAGTTGTTCAAACAGCCAGACATCGGTAAACACTGCCg                                                                   >  2:255995/1‑100 (MQ=255)
                              tCAGCTGCGCCAGCCACTTCTCAACCAGTTGCGGGTTAGCGGGTTTACCCTCCGGCCCCAGCAGTTGTTCAAACAGCCAGACATCGGTAAACACTGCCGa                                                                  <  1:589536/100‑1 (MQ=255)
                                aGCTGCGCCAGCCACTTCTCAACCAGTTGCGGGTTAGCGGGTTTACCCTCCGGCCCCAGCAGTTGTTCAAACAGCCAGACATCGGTAAACACTGCCGaaa                                                                >  2:1182007/1‑100 (MQ=255)
                                aGCTGCGCCAGCCACTTCTCAACCAGTTGCGGGTTAGCGGGTTTACCCTCCGGCCCCAGCAGTTGTTCAAACAGCCAGACATCGGTAAACACTGCCGaaa                                                                >  2:1529850/1‑100 (MQ=255)
                                 gCTGCGCCAGCCACTTCTCAACCAGTTGCGGGTAGCGGGTTTACCCTCCGGCCCCAGCAGTTGTTCAAACAGCCAGAGATCGGTAAACACTGCCGAAAAc                                                              <  2:1759431/100‑1 (MQ=255)
                                  cTGCGCCAGCCACTTCTCAACCAGTTGCGGGTTAGCGGGTTTACCCTCCGGCCCCAGCAGTTGTTCAAACAGCCAGACATCGGTAAACACTGCCGAAAAc                                                              <  1:1843692/100‑1 (MQ=255)
                                  cTGCGCCAGCCACTTCTCAACCAGTTGCGGGTTAGCGGGTTTACCCTCCGGCCCCAGCAGTTGTTCAAACAGCCAGACATCGGTAAACACTGCCGAAAAc                                                              >  1:1565405/1‑100 (MQ=255)
                                     cgcCAGCCACTTCTCAACCAGTTGCGGGTTAGCGGGTTTACCCTCCGGCCCCAGCAGTTGTTCAAACAGCCAGACATCGGTAAACACTGCCGAAAAc                                                              <  2:2474020/97‑1 (MQ=255)
                                      gccagccaCTTCTCAACCAGTTGCGGGTTAGCGGGTTTACCCTCCGGCCCCAGCAGTTGTTCAAACAGCCAGACATCGGTAAACACTGCCGAAAACAGtt                                                          >  2:1143568/1‑100 (MQ=255)
                                        cagccaCTTCTCAACCAGTTGCGGGTTAGCGGGATTGCCCTCAGAACCGAGAAGTATTTCAAACAGCCAGACATAGGTAAACACAGCCGACAACAGt                                                           >  2:2831904/1‑97 (MQ=255)
                                        cagccaCGTCTCAACCAGTTGCGGGTTAGCTAGTTTACCCTCCGGCCCCAGCAGTTGTTCAAACAGCCAGACATCGGTAAACACTGCCGAAAACAGttt                                                         <  2:332681/99‑1 (MQ=255)
                                         agccaCTTCTCAACCAGTTGCGGGTTAGCGGGTTTACCCTCCGGCCCCAGCAGTTGTTCAAACAGCCAGACATCGGTAAACACTGCCGAAAACAGTTTGc                                                       >  2:386953/1‑100 (MQ=255)
                                          gccaCTTCTCAACCAGTTGCGGGTTAGCGGGTTTACCCTCCGGCCCCAGCAGTTGTTCAAACAGCCAGACATCGGTAAACACTGCCGAAAACAGTTTGCg                                                      >  2:2690037/1‑100 (MQ=255)
                                          gccaCTTCTCAACCAGTTGCGGGTTAGCGGGTTTACCCTCCGGCCCCAGCAGTTGTTCAAACAGCCAGACATCGGTAAACACTGCCGAAAACAGTTTGCg                                                      >  2:1403467/1‑100 (MQ=255)
                                           ccaCTTCTCAACCAGTTGCGGGTTAGCGGGTTTACCCTCCGGCCCCAGCAGTTGTTCAAACAGCCAGACATCGGTAAACACTGCCGAAAACAGTTTGCGa                                                     >  1:1895558/1‑100 (MQ=255)
                                            caCTTCTCAACCAGTTGCGGGTTAGCGGGTTTACCCTCCGGCCCCAGCAGTTGTTCAAACAGTCAGACATCGGTAAACACTGCCGAAAACAGTTTGCGAt                                                    <  2:2672741/100‑1 (MQ=255)
                                             aCTTCTCAACCAGTTGCGGGTTAGCGGGTTTACCCTCCGGCCCCAGCAGTTGTTCAAACAGCCAGACATCGGTAAACACTGCCGAAAACAGTTTGCGATa                                                   >  2:1712207/1‑100 (MQ=255)
                                                tctcAACCATTTGCGGGTTATCGGGTTTATCCTCCGGCCCTAGTAGTTGTTCAAACAGCCAGACATCGGTAAACACTGCCGAAAACAGTTTGCGATAATc                                                <  1:939669/100‑1 (MQ=255)
                                                tctcAACCAGTTGCGGGTTAGCGGGTTTACCCTCCGGCCCCAGCAGTTGTTCAAACAGCCAGACATCGGTAAACACTGCCGAAAACAGTTTGCGATAATc                                                <  2:168016/100‑1 (MQ=255)
                                                 ctcAACCAGTTGCGGATTAGCGGATTTACCCTCCGGCCCCAGCAGTTGTTCAAACAGCCAGACATCGGTAACCGCTGCCGAAAACAGTTTGCGATAATc                                                >  2:1804555/1‑99 (MQ=255)
                                                   cAACCAGTTGCGGGTTAGCGGGTTTACCCTCCGGCCCCAGCAGTTGTTCAAACAGCGAGACATCGGTAAACACTGCCGAAAACAGTTTGCGATAATCTTc                                             >  2:1933569/1‑100 (MQ=255)
                                                   cAACCAGTTGCGGGTTAGCGGGTTTACCCTCCGGCCCCAGCAGTTGTTCAAACAGCCAGACATCGGTAAACACTGCCGAAAACAGTTTGCGATAATCTTc                                             >  1:895564/1‑100 (MQ=255)
                                                   cAACCAGTTGCGGGTTAGCGGGTTTACCCTCCGGCCCCAGCAGTTGTTCAAACAGCCAGACATCGGTAAACACTGCCGAAAACAGTTTGCGATAATCTTc                                             >  1:2953034/1‑100 (MQ=255)
                                                    aaCCAGTTGCGGGTTAGCGGGTTTACCCTCCGGCCCCAGCAGTTGTTCAAACAGCCAGACATCGGTAAACACTGCCGAAAACAGTTTGCGATAATCTTcc                                            <  2:373455/100‑1 (MQ=255)
                                                    aaCCAGTTGCGGGTTAGCGGGTTTACCCTCCGGCCCCAGCAGTTGTTCAAACAGCCAGACATCGGTAAACACTGCCGAAAACAGTTTGCGATAATCTTc                                             >  2:2858513/1‑99 (MQ=255)
                                                    aaCCAGTTGCGGGTTAGCGGGTTTACCCTCCGGCCCCAGCAGTTGTTCAAACAGCCAGACATCGGTAAACACTGCCGAAAACACTTTGCGATAATCTTcc                                            >  2:840899/1‑100 (MQ=255)
                                                    aaCCAGTTGCGGGTTAGCGGGTTTACCCTCCGGCCCCAGCAGTTGTTCAAACAGCCAGACAACGGTAAACACTGCCGAAAACAGTTAGCGATAATCTTcc                                            <  2:1868821/100‑1 (MQ=255)
                                                     aCCAGTTGCGGGTTAGCGGGTTTACCCTCCGGCCCCAGCAGTTGTTCAAACAGCCAGACATCGGTAAACACTGCCGAAAACAGTTTGCGATAATCTTCCg                                           >  1:2646141/1‑100 (MQ=255)
                                                       cAGTTGCGGGTTAGCGGGTTTACCCTCCGGCCCCAGCAGTTGTTCAAACAGCCAGACATCGGTAAACACTGCCGAAAACAGTTTGCGATAATCTTCCGgt                                         <  2:1559840/100‑1 (MQ=255)
                                                       cAGTTGCGGGTTAGCGGGTTTACCCTCCGGCCCCAGCAGTTGTTCAAACAGCCAGACATCGGTAAACACTGCCGAAAACAGTTTGCGATAATCTTCCGgt                                         <  2:2622693/100‑1 (MQ=255)
                                                         gTTGCGGGTTAGCGGGTTTACCCTCCGGCCCCAGCAGTTGTTCAAACAGCCAGACATCGGTAAACACTGCCGAAAACAGTTTGCGATAATCTTCCGgttg                                       >  1:870407/1‑100 (MQ=255)
                                                          ttGCTGTTTAGCTGGTTTACCTTTTGGTCTCAGTAGTTGTTCAAACAGCCAGACATCGGTAAACACTGCCGAAAACAGTTTGCGATAATCTTCCGgttgt                                      <  1:1496498/100‑1 (MQ=255)
                                                           tGCGGGTTAGCGGGTTTACCCTCCGGCCCCAGCAGTTGTTCAAACAGCCAGACATCGGTAAACACTGCCGAAAACAGTTTGCGATAATCTTCCGgttgtt                                     <  2:2493882/100‑1 (MQ=255)
                                                            gCGGGTTAGCGGGTTTACCCTCCGGCCCCAGCAGTTGTTCAAACAGCCAGACATCGGTAAACACTGCCGAAAACAGTTTGCGATAATCTTCCGGTTGTTc                                    >  1:982748/1‑100 (MQ=255)
                                                             cGGGTTAGCGGGTTTACCCTCCGGCCCCAGCAGTTGTTCAAACAGCCAGACATCGGTAAACACTGCCGAAAACAGTTTGCGATAATCTTCCGGTTGTTcg                                   >  1:2442801/1‑100 (MQ=255)
                                                               ggTTAGCGGGTTTACCCTCCGGCCCCAGCAGTTGTTCGAACAGCCAGACATCGGTAAACACTGCCGAAAACAGTTTGCGAGAATCTTCCGGTTGTTcgcc                                 <  2:1517737/100‑1 (MQ=255)
                                                               ggTTAGCGGGTTTACCCTCCGGCCCCAGCAGTTGTTCAAACAGCCAGACATCGGTAAACACTGCCGAAAACAGTTTGCGATAATCTTCCGGTTGTTcgcc                                 <  1:1645499/100‑1 (MQ=255)
                                                                gTTAGCGGGTTTACCCTCCGGCCCCAGCAGTTGTTCAAACAGCCAGACATCGGTAAACACTGCCGAAAACAGTTTGCGATAATCTTCCGGTTGTTcgccg                                >  1:2400712/1‑100 (MQ=255)
                                                                 ttAGCGGGTTTACCCTCCGGCCCCAGCAGTTGTTCAAACAGCCAGACATCGGTAAACACTGCCGAAAACAGTTTGCGATAATCTTCCGGTTGTTcgccgc                               <  1:742052/100‑1 (MQ=255)
                                                                  tAGCGGGTTTACCCTCCGGCCCCAGCAGTTGTTCAAACAGCCAGACATCGGTAAACACTGCCGAAAACAGTTTGCGATAATCTTCCGGTTGTTCGCCGCt                              <  1:2328763/100‑1 (MQ=255)
                                                                      gggTTTACCCTCCGGCCCCAGCAGTTGTTCAAACAGCCAGACATCGGTAAACACTGCCGAAAACAGTTTGCGATAATCTTCCGGTTGTTCGCCGCTGACa                          <  1:1933592/100‑1 (MQ=255)
                                                                       ggTTTACCCTCCGGCCCCAGCAGTTGTTCAAACAGCCAGACATCGGTAAACACTGCCGAAAACAGTTTGCGATAATCTTCCGGTTGTTCGCCGCTGACAg                         >  1:1249307/1‑100 (MQ=255)
                                                                         tttACCCTCCGGCCCCAGCAGTTGTTCAAACAGCCAGACATCTGTAAACACTGCCGAAAACAGTTTGCGATAATCTTCCGGTTGTTCGCCGCTGACAGGt                       <  1:2248535/100‑1 (MQ=255)
                                                                         tttACCCTCCGGCCCCAGCAGTTGTTCAAACAGCCAGACATCGGTAAACACTGCCGAAAACAGTTTGCGATAATCTTCCGGTTGTTCGCCGCTGACAGGt                       >  1:2119207/1‑100 (MQ=255)
                                                                          ttACCCTCCGGCCCCAGCAGGTGTTCAAACAGCCAGACATCGGTAAACACTGCCGAAAACAGTTTGCGATAATCTTCCGGTTGTTCGCCGCTGACAGGtt                      <  2:975164/100‑1 (MQ=255)
                                                                           tACCCTCCGGCCCCAGCAGTTGTTCAAACAGCCAGACATCGGTAAACACTGCCGAAAACAGTTTGCGATAATCTTCCGGTTGTTCGCCGCTGACAGGttt                     >  2:933392/1‑100 (MQ=255)
                                                                            aCCCTCCGGCCCCAGCAGTTGTTCAAACAGCCAGACATCGGTAAACACTGCCGAAAACAGTTTGCGATAATCTTCCGGTTGTTCGCCGCTGACAGGTTTg                    >  2:120582/1‑100 (MQ=255)
                                                                            aCCCTCCGGCCCCAGCAGTTGTTCAAACAGCCAGACATCGGTAAACACTGCCGAAAACAGTTTGCGATAATCTTCCGGTTGTTCGCCGCTGACAGGTTTg                    <  2:1645073/100‑1 (MQ=255)
                                                                             cccTCCGGCCCCAGCAGTTGTTCAAACAGCCAGACATCGGTAAACACTGCCGAAAACAGTTTGCGATAATCTTCCGGTTGTTCGCCGCTGACAGGTTTGc                   <  1:3010982/100‑1 (MQ=255)
                                                                             cccTCCGGCCCCAGCAGTTGTTCAAACAGCCAGACATCGGTAAACACTGCCGAAAACAGTTTGCGATAATCTTCCGGTTGTTCGCCGCTGACAGGTTTGc                   >  2:2713997/1‑100 (MQ=255)
                                                                             cccTCCGGCCCCAGCAGTTGTTCAAACAGCCAGACATCGGTAAACACTGCCGAAAACAGTTTGCGATAATCTTCCGGTTGTTCGCCGCTGACAGGTTTGc                   <  2:1565387/100‑1 (MQ=255)
                                                                             cccTCCGGCCCCAGCAGTTGTTCAAACAGCCAGACAGCGGTAAACACTGCCGAAAACAGTTTGCGATAATCTTCCGGTTGTTCGCCGCTGACAGGTTTGc                   >  1:2142881/1‑100 (MQ=255)
                                                                              ccTCCGGCCCCAGCAGTTGTTCAAACAGCCAGACATCGGTAAACACTGCCGAAAACAGTTTGCGATAATCTTCCGGTTGTTCGCCGCTGACAGGTTTGcc                  <  2:415914/100‑1 (MQ=255)
                                                                                 ccGGCCCCAGCAGTTGTTCAAACAGCCAGACATCGGTAAACACTGCCGAAAACAGTTTGCGATAATCTTCCGGTTGTTCGCCGCTGACAAGTTTGCCATc               >  2:1228820/1‑100 (MQ=255)
                                                                                      cccAGCAGTTGTTCAAACAGCCAGACATCGGTAAACACTGCCGAAAACAGTTTGCGATAATCTTCCGGTTGTTCGCCGCTGACAGGTTTGCCATCCAGCa          >  2:357766/1‑100 (MQ=255)
                                                                                       ccAGCAGTTGTTCAAACAGCCAGACATCGGTAAACACTGCCGAAAACAGTTTGCGATAATCTTCCGGTTGTTCGCCGCTGACAGGTTTGCCATCCAGCaa         >  2:161821/1‑100 (MQ=255)
                                                                                          gcagTTGTTCAAACAGCCAGACATCGGTAAACACTGCCGAAAACAGTTTGCGATAATCTTCCGGTTGTTCGCCGCTGACAGGTTTGCCATCCAGCAAGAt      <  1:2702349/100‑1 (MQ=255)
                                                                                           cagTTGTTCAAACAGCCAGACATCGGTAAACACTGCCGAAAACAGTTTGCGATAATCTTCCGGTTGTTCGCCGCTGACAGGTTTGCCATCCAGCAAGAt      <  1:729163/99‑1 (MQ=255)
                                                                                             gTTGATCGAACAGCCAGACATCGGTAAACACTGCCGTAAACGGTTTGCTATAATCTTCCGGTTGTTCGCCGATGTCAGCTTTGCCATCCAGCAAGATTTc   <  2:1224278/100‑1 (MQ=255)
                                                                                              ttGTTCAAACAGCCAGACATCGGTAAACACTGCCGAAAACAGTTTGCGATAATCTTCCGGTTGTTCGCCGCTGACAGGTTTGCCATCCAGCAAGATTTcg  <  2:840841/100‑1 (MQ=255)
                                                                                                 |                                                                                                
CGTTGCTTAACTCAAGCTTATGAGCCATTTTCAGCTGCGCCAGCCACTTCTCAACCAGTTGCGGGTTAGCGGGTTTACCCTCCGGCCCCAGCAGTTGATCAAACAGCCAGACATCGGTAAACACTGCCGAAAACAGTTTGCGATAATCTTCCGGTTGTTCGCCGCTGACAGGTTTGCCATCCAGCAAGATTTCG  >  NC_000913/2307289‑2307482

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: