BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	2,361,323	T→A	100%	Q31L (CAG→CTG)	rhmD ←	L‑rhamnonate dehydratase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,361,323	0	T	A	98.4%	391.0 / ‑2.9	119	Q31L (CAG→CTG)	rhmD	L‑rhamnonate dehydratase
Reads supporting (aligned to +/- strand): ref base T (2/0); new base A (57/60); total (59/60)
Fisher's exact test for biased strand distribution p-value = 2.44e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.72e-01

Only 100 of 119 total aligned reads displayed.

CAAGAACGTTAATGCCAAATGACTGGCGTGACTGCTCGTAATCGCGGTATTTACTCATCGGGGTGGCAATATGATCGTCTATCCAGTGATTCGCCCCCTGGTCGTGATAATCACCGCCGCCAGCGCCTTTTTCTGCTGTCGCACCGCCAGTAAACCAGGCGCGAACCTGTTTAATTTTTGGTAGGGTCATGATGT  >  NC_000913/2361225‑2361419

 aaGAACGTTAATTCCAAATGACTGGCGTGACTGCTCGTAATCGCGGTATTTACTCATCGGGGTGGCAATATGATCGTCTATCCAGTGATTCGCCCCCAgg                                                                                                <  1:2957893/100‑1 (MQ=255)

     aCGTTAATGCCAAATGACTGGCGTGACTGCTCGTAATCGCGGTATTTACTCATCGGGGTGGCAATATGATCGTCTATCCAGTGATTCGCCCCCAGGTCGt                                                                                            <  1:1940196/100‑1 (MQ=255)

     aCGTGAATGCCAAATGACTGGCGTGACTGCTCGTAATCGCGGTATTTACTCATCGGGGTGGCAATATGATCGTCTATCCAGTGATTCGCCTCCAGGTc                                                                                              <  2:2770326/98‑1 (MQ=255)

      cGTTAATGCCAAATGACTGGCGTGACTGCTCGTAATCGCGGTATTTACTCATCGGGGTGGCAATATGATCGTCTATCCAGTGATTCGCCCCCAGGTCGTg                                                                                           <  1:3017334/100‑1 (MQ=255)

        ttAATGCCAAATGACTTGCGTGACTGCTCGTAATCGTGGTATTTACTCATCGGGGTGGCAATATGATCGTCTATCCAGTGATTCGCCCCCAGGTCGTGAt                                                                                         <  1:157984/100‑1 (MQ=255)

         tAATGCCAAATGTCTTGCGTGACTGCTCTTAATCGCGGTATTTACTCATCGGGGTGGCAATATGATCGTCTATCCATTGATTCGCCCCCAGGTCGTCAt                                                                                         <  2:2189191/99‑1 (MQ=255)

          aaTGCCAAATGACTGGCGTGACTGCTCGTAATCGCGGTATTTACTCATCGGGGTGGCAATATGATCGTCTATCCAGTGATTCGCCCCCAGGTCGTGATaa                                                                                       <  2:763146/100‑1 (MQ=255)

          aaTGCCAAATGACTGGCGTGACTGCTCGTAATCGCGGTATTTACTCATCGGGGTGGCAATATGATCGTCTATCCAGTGATTCGCCCCCAGGTCGTGATaa                                                                                       >  1:1785119/1‑100 (MQ=255)

            tGCCAAATGACTGGCGTGACTGCTCGTAATCGCGGTATTTACTCATCGGGGTGGCAATATGATCGTCTATCCAGTGATTCGCCCCCAGGTCGTGATAATc                                                                                     >  2:2022019/1‑100 (MQ=255)

              ccAAATGACTGGCGTGACTGCTCGTAATCGCGGTATTTACTCATCGGGGTGGCAATATGATCGTCTATCCAGTGATTCGCCCCCAGGTCGTGATAATCAc                                                                                   >  2:2365568/1‑100 (MQ=255)

               cAAATGACTGGCGTGACTGCTCGTAATCGCGGTATTTACTCATCGGGGTGGCAATATGATCGTCTATCCAGTGATTCGCCCCCAGGTCGTGATAATCAcc                                                                                  <  1:1606031/100‑1 (MQ=255)

               cAAATGACTGGCGTGACTGCTCGTAATCGCGGTATTTACTCATCGGGGTGGCAATATGATCGTCTATCCAGTGATTCGCCCCCAGGTCGTGATAATCAcc                                                                                  >  1:527161/1‑100 (MQ=255)

                 aaTGACTGGCGTGATTGCTCGTAATCGCGGTATTTACTCATCGGGGTGGCAATATGATCGTCTATCCAGTGATTCGCCCCCAGGTCGTGATAATCAccgc                                                                                <  1:636373/100‑1 (MQ=255)

                  aTGACTGGCGTGACTGCTCGTAATCGCGGTATTTACTCATCGGGGTGGCAATATGATCGTCTATCCAGTGATTCGCCCCCAGGTCGTGATAATCAccgcc                                                                               <  2:1424233/100‑1 (MQ=255)

                     aCTGGCGTGACTGCTCGTAATCGCGGTATTTACTCATCGGTGTGGCAATATGATCGTCTATCCAGTGATTCGCCCCCAGGTCGTGATAATCACCgccgcc                                                                            <  1:846736/100‑1 (MQ=255)

                      cTGGCGTGACTGCTCGTAATCGCGGTATTTACTCATCGGGGTGGCAATATGATCGTCTATCCAGTGATTCGCCCCCAGGTCGTGATAATCACCGCCGCCa                                                                           <  1:3023408/100‑1 (MQ=255)

                        ggCGTGACTGCTCGTAATCGCGGTATTTACTCATCGGGGTGGCAATATGATCGTCTATCCAGTGATTCGCCCCCAGGTCGTGATAATCACCGCCGCCAgc                                                                         >  2:2478475/1‑100 (MQ=255)

                        ggCGTGACTGCTCGTAATCGCGGTATTTACTCATCGGGGTGGCAATATGATCGTCTATCCAGTGATTCGCCCCCAGGTCGTGATAATCACCGCCGCCAgc                                                                         >  1:347256/1‑100 (MQ=255)

                        ggCGTGACTGCTCGTAATCGCGGTATTTACTCATCGGGGTGGCAATATGATCGTCTATCCAGTGATTCGCCCCCAGGTCGTGATAATCACCGCCGCCAgc                                                                         >  2:126391/1‑100 (MQ=255)

                        ggCGTGACTGCTCGTAATCGCGGTATTTACTCATCGGGGTGGCAATATGATCGTCTATCCAGTGATTCGCCCCCAGGTCGTGATAATCACCACCGCCAgc                                                                         >  1:1494977/1‑100 (MQ=255)

                         gCGTGACTGCTCGTAATCGCTGTATTTATTTTTCGTGGTGGCAATATGATCGTCTATCCAGTGATTCGCCCCCAGGTCGTGATAATCACCGCCGCCAgcg                                                                        <  1:963528/100‑1 (MQ=255)

                          cGTGACTGCTCGTAATCGCGGTATTTACTCATCGGGGTGGCAATATGATCGTCTATCCAGTGATTCGCCCCCAGGTCGTGATAATCACCGCCGCCAgcgc                                                                       >  1:1154571/1‑100 (MQ=255)

                          cGTGACTGCTCGTAATCGCGGTATTTACTCATCGGGGTGGCAATATGATCGTCTATCCAGTGATTCGCCCCCAGGTCGTGATAATCACCGCCGCCAgcgc                                                                       >  2:1115405/1‑100 (MQ=255)

                          cGTGACTGCTCGTAATCGCGGTATTTACTCATCGGGGTGGCAATATGATCGTCTATCCAGTGATTCGCCCCCAGGTCGTGATAATCACCGCCGCCAgcg                                                                        >  1:102634/1‑99 (MQ=255)

                                 gCTCGTAATCGCGGTATTTACTCATCGGGGTGGCAATATGATCGTCTATCCAGTGATTCGCCCCCAGGTCGTGATAATCACCGCCGCCAGCGCCttttt                                                                 >  1:2193178/1‑99 (MQ=255)

                                 gCTCGTAATCGCGGTATTTACTCATAGGGGTGGCAATATGATCGTCTATCCAGTGATTCGCCCCCAGGTCGTGATAATCACCGCCGCCAGCGCCTTTTTc                                                                <  2:782773/100‑1 (MQ=255)

                                 gCTCGTAATCGCGGTATTTACTAATCGGGGTGGCAATATGATCGTCTATCCAGTGATTCGCCCCCAGGTTGTGATAGTCACCGCCGCGAGCGCCttttt                                                                 >  2:2849855/1‑99 (MQ=255)

                                  cTCGTAATCGCGGTATTTACTCATCGGGGTGGCAATATGATCGTCTATCCAGTGATTCGCCCCCAGGTCGTGATAATCACCGCCGCCAGCGCCTTTTTct                                                               >  1:2820427/1‑100 (MQ=255)

                                   tCGTAATCGCGGTATTTACTCATCGGGGTGGCAATATGATCGTCTATCCAGTGATTCGCCCCCAGGTCGTGATAATCACCGCCGCCAGCGCCTTTTTctg                                                              >  2:1103834/1‑100 (MQ=255)

                                   tCGTAATCGCGGTATTTACTCATCGGGGTGGCAATATGATCGTCTATCCAGTGATTCGCCCCCAGGTCGTGATAATCACCGCCGCCAGCGCCTTTTTctg                                                              <  1:2022042/100‑1 (MQ=255)

                                    cGTAATCGCGGTATTTACTCATCGTGGTGGCAATATGATCGTCTATCCAGTGATTCGCCCCCAGGTCGTGATAATCACCGCCGCCAGCGCCTTTTCctgc                                                             <  1:1237120/100‑1 (MQ=255)

                                    cGTAATCGCGGTATTTACTCATCGGGGTGGCAATATGATCGTCTATCCAGTGATTCGCCCCCAGGTCGTGATAATCACCGCCGCCAGCGCCTTTTTctgc                                                             <  2:347253/100‑1 (MQ=255)

                                    cGTAATCGCGGTATTTACTCATCGGGGTGGCAATATGATCGCCTATCCAGTGATTCGCCCCCAGGTCGTGATAATCACCGCCGCCAGCGCCTTTTTctgc                                                             <  1:1730095/100‑1 (MQ=255)

                                     gTAATCGCGGTATTTACTCATCGGGGTGGCAATATGTTTGTCTATCCAGTGATTCGCCCCCAGGTCGTGATAATCACCGCCGCCAGCGCCTTTTTctgc                                                             <  1:981861/99‑1 (MQ=255)

                                     gTAATCGCGGTATTTACTCATCGGGGTGGCAATATGATCGTCTATTCAGTGATTCGCCCCCAGGTCGTGATAATCACCGCCGCCAGCGCCTTTTTctgct                                                            <  1:2331403/100‑1 (MQ=255)

                                     gTAATCGCGGTATTTACTCATCGGGGTGGCAATATGATCGTCTATCCAGTGATTCGCCCCCAGGTCGTGATAATCACCGCCGCCAGCGCCTTTTTctgct                                                            <  2:218616/100‑1 (MQ=255)

                                     gTAATCGCGGTATTTACTCATCGGGGTGGCAATATGATCGTCTATCCAGTGATTCGCCCCCAGGTCGTGATAATCACCGCCGCCAGCGCCTTTTTctgct                                                            <  1:406969/100‑1 (MQ=255)

                                      tAATCGCGGTATTTACTCATCGGGGTGGCAATATGATCGTCTATCCAGTGATTCGCCCCCAGGTCGTGATAATCACCGCCGCCAGCGCCTTTTTctgctg                                                           >  1:2984487/1‑100 (MQ=255)

                                       aaTCGCGGTATTTACTCATCGGGGTGGCAATATGATCGTCTATCCAGTGATTCGCCCCCAGGTCGTGATAATCACCGCCGCCAGCGCCTTTTTCTGCTGt                                                          >  1:524348/1‑100 (MQ=255)

                                        aTCGCGGTATTTACTCATCGGGGTGGCAATATGATCGTCTATCCAGTGATTCGCCCCCAGGTCGTGATAATCACCGCCGCCAGCGCCTTTTTCTGCTGTc                                                         <  2:515750/100‑1 (MQ=255)

                                         tCGCGGTATTTACTCATCGGGGTGGCAATATGATCGTCTATCCAGTGATTCGCCCCCAGGTCGTGATAATCACCGCCGCCAGCGCCTTTTTCTGCTGTCg                                                        <  1:2710363/100‑1 (MQ=255)

                                           gcgGTATTTACTCATCGGGGTGGCAATATGATCGTCTATCCAGTGATTCGCCCCCAGGTCGTGATAATCACCGCCGCCAGCGCCTTTTTCTGCTGTCGCa                                                      <  1:1060947/100‑1 (MQ=255)

                                            cgGTATTTACTCATCGGGGTGGCAATATGATCGTCTATCCAGTGATTCGCCCCCAGGTCGTGATAATCACCGCCGCCAGCGCCTTTTTCTGCTGTCGCAc                                                     >  1:2475564/1‑100 (MQ=255)

                                              gTATTTACTCATCGGGGTGGCAATATGATCGTCTATCCAGTGATTCGCCCCCAGGTCGTGATAATCACCGCCGCCAGCGCCTTTTTCTGCTGTCGCACCg                                                   >  1:753391/1‑100 (MQ=255)

                                                aTTTACTCATCGGGGTGGCAATATGATCGTCTATCCAGTGATTCGCCCCCAGGTCGTGATAATCACCGCCGCCAGCGCCTTTTTCTGCTGTCGCACCGcc                                                 <  1:2882466/100‑1 (MQ=255)

                                                  ttACTCATCGGGGTGGCAATATGATCGTCTATCCAGTGATTCGCCCCCAGGTCGTGATAATCACCGCCGCCAGCGCCTTTTTCTGCTGTCGCACCGCCAg                                               >  2:1327976/1‑100 (MQ=255)

                                                    aCTCATCGTGGTGGCAATATGATCGTCTATCCAGTGATTCGCCCCCAGGTCGTGGTAATCACCGCCGCCAGCGCCTTTTTCTGCTGTCGCACCGCCAGTa                                             <  1:728069/100‑1 (MQ=255)

                                                     cTCATCGGGGTGGCAATATGATCGTCTATCCAGTGATTCGCCCCCAGGTCGTGATAATCACCGCCGCCAGCGCCTTTTTCTGCTTTCGCACCGCCAGTaa                                            >  2:620048/1‑100 (MQ=255)

                                                       cATCGGGGTGGCAATATGATCGTCTATCCAGTGATTCGCCCCCAGGTCGTGATAATCACCGCCGCCAGCGCCTTTTTCTGCTGTCGCACCGCCAGTAAAc                                          >  1:502806/1‑100 (MQ=255)

                                                       cATCGGGGTGGCAATATGATCGTCTATCCAGTGATTCGCCCCCAGGTCGTGATAATCACCGCCGCCAGCGCCTTTTTCTGCTGTCGCACCGCCAGTAAAc                                          >  2:40460/1‑100 (MQ=255)

                                                       cATCGGGGTGGCAATATGATCGTCTATCCAGTGATTCGCCCCCAGGTCGTGATAATCACCGCCGCCAGCGCCTTTTTCTGCTGTCGCACCGCCAGTAAAc                                          >  2:1163807/1‑100 (MQ=255)

                                                       cATCGGGGTGGCAATATGATCGTCTATCCAGTGATTCGCCCCCAGGTCGTGATAATCACCGCCGCCAGCGCCTTTTTCTGCTGTCGCACCGCCAGTAAAc                                          <  1:2600693/100‑1 (MQ=255)

                                                          cGGGGTGGCAATATGATCGTCTATCCAGTGATTCGCCCCCAGGTCGTGATAATCACCGCCGCCAGCGCCTTTTTCTGCTGTCGCACCGCCAGTAAACCAg                                       >  2:2341043/1‑100 (MQ=255)

                                                            gggTGGCAATATGATCGTCTATCCAGTGATTCGCCCCCAGGTCGTGATAATCACCGCCGCCAGCGCCTTTTTCTGCTGTCGCACCGCCAGTAAACCAGgc                                     >  1:917992/1‑100 (MQ=255)

                                                             ggTGGCAATATGATCGTCTATCCAGTGATTCGCCCCCAGGTCGTGATAATCACCGCCGCCAGCGCCTTTTTCTGCTGTCGCACCGCCAGTAAACCAGgcg                                    <  1:742155/100‑1 (MQ=255)

                                                             ggTGGCAATATGATCGTCTATCCAGTGATTCGCCCCCAGGTCGTGATAATCACCGCCGCCAGCGCCTTTTTCTGCTGTCGCACCGCCAGTAAACCAGgcg                                    <  1:2913607/100‑1 (MQ=255)

                                                                ggCAATATGATCGTCTATCCAGTGATTCGTCCCCAGGTCGTGATAATCACCGCCGCCAGCGCCTTTTTCTGCTGTCGCACCGCCAGTAAACCAGGCGCGa                                 <  1:1477721/100‑1 (MQ=255)

                                                                 gCAATATGATCGTCTATCCAGTGATTCGCCCCCAGGTCGTGATAATCACCGCCTCCAGCGCCTTTTTCTGCTGTAAAACCACCAGTAAACCAGGCGCGaa                                >  1:539071/1‑100 (MQ=255)

                                                                 gCAATATGATCGTCTATCCAGTGATTCGCCCCCAGGTCGTGATAATCACCGCCGCCAGCGCCTTTTTCTGCTGTCGCACCGCCAGTAAACCAGGCGCGaa                                >  1:2349176/1‑100 (MQ=255)

                                                                  cAATATGGTGGTCTATCCAGTGATTCTCCCCCAGATCGTGATAATCACCGCCGCTAGCGCCTTTTTCTGCTGTCGCACCGCCAGTAAACAAGGCGCGa                                 <  2:3016035/98‑1 (MQ=255)

                                                                  cAATATGATCGTCTATCCAGTGATTCGCCCCCAGGTCGTGATAATCACCGCCGCCAGCGCCTTTTTCTGCTGTCGCACCGCCAGTAAACCAGGCGCGaa                                >  2:479470/1‑99 (MQ=255)

                                                                  cAATATGATCGTCTATCCAGTGATTCGCCCCCAGGTCGTGATAATCACCGCCGCCAGCGCCTTTTTCTGCTGTCGCACCGCCAGTAAACCAGGCGCGAAc                               >  1:1336123/1‑100 (MQ=255)

                                                                  cAATATGATCGTCTATCCAGTGATTCGCCCCCAGGTCGTGATAATCACCGCCGCCAGCGCCTTTTTCTGCTGTCGCACCGCCAGTAAACCAGGCGCGAAc                               >  1:2548289/1‑100 (MQ=255)

                                                                  cAATATGATCGTCTATCCAGTGATTCGCCCCCAGGTCGTGATAATCACCGCCGCCAGCGCCTTTTTCTGCTGTCGCACCGCCAGTAAACCAGGCGCGAAc                               >  2:75886/1‑100 (MQ=255)

                                                                  cAATATGATCGTCTATCCAGTGATTCGCCCCCAGGTCGTGATAATCACCGCCGCCAGCGCCTTTTTCTGCTGTCGCACCGCCAGTAAACCAGGCGCGAAc                               >  2:1865558/1‑100 (MQ=255)

                                                                  cAATATGATCGTCTATCCAGTGATTCGCCCCCAGGTCGTGATAATCACCGCCGCCAGCGCCTTTTTATGCTGTCGCACCGCCAGTAAACCAGGCGCGAAc                               >  1:1511544/1‑100 (MQ=255)

                                                                  cAATATGATCGTCTATCCAGAGATTCGCCCCCAGGTCGTGATAATCACCGCCGCCAGCGCCTTTTTCTGCTGTCGCACCCCCAGTAAACCAGCCACGac                                >  2:1853702/1‑98 (MQ=255)

                                                                   aaTATGATCGTCTATCCAGTGATTCGCCCCCAGGTCGTGATAATCACCGCCGCCAGCGCCTTTTTCTGCTGTCGCACCGCCAGTAAACCAGGCGCGAAcc                              <  1:1131887/100‑1 (MQ=255)

                                                                     tatGATCGTCTATCCAGTGATTCGCCCCCAGGTCGTGATAATCACCGCCGCCAGCGCCTTTTTCTGCTGTCGCACCGCCAGTAAACCAGGCGCGAACCTg                            >  2:2202747/1‑100 (MQ=255)

                                                                       ttaTCTTCTATACAGTTATTCGCCCCTAGTTCCTGATAATTACCGCCGCCATCGCCTTTTTCTGCTGTCTCACCTTCAGTAAACCAGGCGCGAACCTGtt                          <  2:488859/98‑1 (MQ=255)

                                                                       tGATCGTCTATCCAGTGATTCGCCCCCAGGTCGTGATAATCACCGCCGCCAGCGCCTTTTTCTGCTGTCGCACCGCCAGTAAACCAGGCGCGAACCTGtt                          >  2:2910353/1‑100 (MQ=255)

                                                                       tGATCGTCTATCCAGTGATTCGCCCCCAGGTCGTGATAATCACCGCCGCCAGCGCCTTTTTCTGCTGTCGCACCGCCAGTAAACCAGGCGCGAACCTGt                           >  2:2794782/1‑99 (MQ=255)

                                                                       tGATCGTCTATCCAGTGATTCGCCCCCAGGTCGTGATAATCACCGCCGCCAGCACCTTTTTCTGCTGTCGCACCGCCAGTAAACCATGCGCGAACCTGtt                          >  1:1758569/1‑100 (MQ=255)

                                                                        gATCGTCTATCCAGTGATTCGCCCCCAGGTCGTGATAATCACCGCCGCCAGCGCCTTTTTCTGCTGTCGCACCGCCAGTAAACCAGGCGCGAACCTGttt                         <  2:461260/100‑1 (MQ=255)

                                                                        gATCGTCTATCCAGTGATTCGCCCCCAGGTCGTGATAATCACCGCCGCCAGCGCCTTTTTCTGCTGTCGCACCGCCAGTAAACCAGGCGCGAACCTGttt                         >  1:1070661/1‑100 (MQ=255)

                                                                        gATCGTCTATCCAGTGATTCGCCCCCAGGTCGTGATAATCACCGCCGCCAGCGCCTTTTTCTGCTGTCGCACCGCCAGTAAACCAGGCGCGAACCTGttt                         <  2:1910568/100‑1 (MQ=255)

                                                                        gATCGTCTATCCAGTGATTCGCCCCCAGGTCGTGATAATCACCGCCGCCAGCGCCTTTTTCTGCTGTCGCACCGCCAGTAAACCAGGCGCGAACCTGttt                         <  1:2590702/100‑1 (MQ=255)

                                                                        gATCGTCTATCCAGTGATTCACCCCCAGGTCGTGATAATCACCGCCGCCAGCGCCTTTTTCTGCTGTCGCACCGCCAGTAAACCAGGCGCGAACCTGtt                          >  2:2477262/1‑99 (MQ=255)

                                                                               tATCCAGTGATTCGCCCCCAGGTCGTGATAATCACCGCCGCCAGCGCCTTTTTCTGCTGTCGCACCGCCAGTAAACCAGGCGCGAACCTGTTTAAttttt                  >  1:1627009/1‑100 (MQ=255)

                                                                                aTCCAGTGATTCGCCCCCAGGTCGTTATAATCACCGCCGCCAGCGCCTTTTTCTGCTGTCGCACCGCCAGTAAACCAGGCGCGAACCTGTTTAATTTTTg                 <  1:299523/100‑1 (MQ=255)

                                                                                aTCCAGTGATTCGCCCCCAGGTCGTGATAATCACCGCTGCCAGCGCCTTTTTCTGCTGTCGCACCGCCAGTAAACCAGGCGCGAACCTGTTTAATTTTTg                 <  1:304196/100‑1 (MQ=255)

                                                                                aTCCAGTGATTCGCCCCCAGGTCGTGATAATCACCGCCGCCAGCGCCTTTTTCTGCTGTCGCACCGCCAGTAAACCAGGCGCGCACCTGTTTAATTTTTg                 >  2:1209289/1‑100 (MQ=255)

                                                                                aTCCAGTGATTCGCCCCCAGGTCGTGATAATCACCGCCGCCAGCGCCTTTTTCTGCTGTCGCACCGCCAGTAAACCAGGCGCGAACCTGTTTAATTTTTg                 >  2:1159907/1‑100 (MQ=255)

                                                                                 tCCAGTGATTCGCCCCCTGGTCGTGATAATCACCGCCGCCAGCGCCTTTTTCTGCTGTCGCACCGCCAGTAAACCAGGCGCGAACCTGTTTAATTTTTgg                >  1:2392870/1‑100 (MQ=255)

                                                                                 tCCAGTGATTCGCCCCCAGGTCGTGATAATCACCGCCGCCAGCGCCTTTTTCTGCTGTCGCACCGCCAGTAAACCAGGCGCGAACCTGTTTAATTTTTgg                >  1:2154680/1‑100 (MQ=255)

                                                                                 tCCAGTGATTCGCCCCCAGGTCGTGATAATCACCGCCGCCAGCGCCTTTTTCTGCTGTCGCACCGCCAGTAAACCAGGCGCGAACCTGTTTAATTTTTgg                <  1:2341070/100‑1 (MQ=255)

                                                                                 tCCAGTGATTCGCCCCCAGGTCGTGATAATCACCGCCGCCAGCGCCTTTTTCTGCTGTCGCACCGCCAGTAAACCAGGCGCGAACCTGTTTAATTTTTgg                <  1:2282204/100‑1 (MQ=255)

                                                                                  ccAGTGATTCGCCCCCAGGTCGTGATAATCACCGCCGCCAGCGCCTTTTTCTGCTGTCGCACCGCCAGTAAACCAGGCGCGAACCTGTTTAATTTTTGGt               <  2:2104610/100‑1 (MQ=255)

                                                                                   cAGTGATTCGCCCCCAGGTCGTGATAATCACCGCCGCCAGCGCCTTTTTCTGCTGTCGCACCGCCAGTAAACCAGGCGCGAACCTGTTTAATTTTTGGTa              >  2:2812435/1‑100 (MQ=255)

                                                                                   cAGTGATTCGCCCCCAGGTCGTGATAATCACCGCCGCCAGCGCCTTTTTCTGCTGTCGCACCGCCAGTAAACCAGGCGCGAACCTGTTTAATTTTTGGTa              <  1:1997685/100‑1 (MQ=255)

                                                                                     gTGATTCGCCCCCAGGTCGTGATAATCACCGCCGCCAGCGCCTTTTTCTGCTGTCGCACCGCCAGTAAACCAGGCGCGAACCTGTTTAATTTTTGGTAgg            <  2:1923794/100‑1 (MQ=255)

                                                                                      tGATTCGCCCCCAGGTCGTGATAATCACCGCCGCCAGCGCCTTTTTCTGCTGTCGCACCGCCAGTAAACCAGGCGCGAACCTGTTTAATTTTTGGTAggg           >  1:865966/1‑100 (MQ=255)

                                                                                        aTTCGCCCCCAGGTCGTGATAATCACCGCCGCCAGCGCCTTTTTCTGCTGTCGCACCGCCAGTAAACCAGGCGCGAACCTGTTTAATTTTTGGTAGGGTc         >  2:139089/1‑100 (MQ=255)

                                                                                        aTTCGCCCCCAGGTCGTGATAATCACCGCCGCCAGCGCCTTTTTCTGCTGTCGCACCGCCAGTAAACCAGGCGCGAACCTGTTTAATTTTTGGTAGGGTc         <  2:1336111/100‑1 (MQ=255)

                                                                                           cGCCCCCAGGTCGTGATAATCACCGCCGCCAGCGCCTTTTTCTGCTGTCGCACCGCCAGTAAACCAGGCGCGAACCTGTTTAATTTTTGGTAGGGTCatg      <  2:1593231/100‑1 (MQ=255)

                                                                                            gCCCCCAGGTCGTGATAATCACCGCCGCCAGCGCCTTTTTCTGCTGTCGCACCGCCAGTAAACCAGGCGCGAACCTGTTTAATTTTTGGTAGGGTCatga     >  1:2612143/1‑100 (MQ=255)

                                                                                             cccccAGGTCGTGATAATAAACGCCGCCAGCGACTTTTTCTGCTGTCGCACCGCCAGCAAACCAGGCGCGACCCTGTTTAATTCTTGGTAGGGTAatga     >  2:2130019/1‑99 (MQ=255)

                                                                                              ccccAGGTCGTGATAATCACCGCCGCCAGCGCCTTTTTCTGCTGTCGCACCGCCAGTAAACCAGGCGCGAACCTGTTTAATTTTTGGTAGGGTCatgatg   <  2:731335/100‑1 (MQ=255)

                                                                                              ccccAGGTCGTGATAATCACCGCCGCCAGCGCCTTTTTCTGCTGTAGCACCACCAGTAAACCAGACGCGAACCTGTTTAATTTTTGGTAGGGTCatga     >  1:56920/1‑98 (MQ=255)

                                                                                               cccAGGTCGTGATAATCACCGCCGCCAGCGCCTTTTTCTGCTGTCGCACCGCCAGTAAACCAGGCGCGAACCTGTTTAATTTTTGGTAGGGTCATGATGt  <  1:2658109/100‑1 (MQ=255)

CAAGAACGTTAATGCCAAATGACTGGCGTGACTGCTCGTAATCGCGGTATTTACTCATCGGGGTGGCAATATGATCGTCTATCCAGTGATTCGCCCCCTGGTCGTGATAATCACCGCCGCCAGCGCCTTTTTCTGCTGTCGCACCGCCAGTAAACCAGGCGCGAACCTGTTTAATTTTTGGTAGGGTCATGATGT  >  NC_000913/2361225‑2361419

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`