Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,386,365 T→A 100% F169Y (TTC→TAC)  yfbL → putative M28A family peptidase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,386,3650TA96.9% 384.2 / 0.4 122F169Y (TTC→TAC) yfbLputative M28A family peptidase
Reads supporting (aligned to +/- strand):  ref base T (2/2);  new base A (59/59);  total (61/61)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 123 total aligned reads displayed.

GTGTGGCAGGATTACTCGAACTGGCACGTTTGTTACATCAGCAAGTACCGAAAACAGGCGTGCAGCTGGTCGCCTATGCGTCGGAAGAACCGCCCTTCTTTCGTAGCGATGAAATGGGGAGCGCGGTGCATGCAGCTTCGCTTGAGCGTCCAGTGAAATTAATGATAGCACTGGAGATGATTGGCTATTACG  >  NC_000913/2386269‑2386460
                                                                                                |                                                                                               
gtgtGGCAGGATTACTCGAACTGGCACGTTTGTTACATCAGCAAGTACCGAAAACAGGCGTGCAGCTGGTCGCCTATGCGTCGGAAGAACCGCCctactt                                                                                              >  2:1645213/1‑100 (MQ=255)
   tgGCAGTATTACTCGAACTGGCACGTTTGTTACATCAGCAAGTACCGAAAACAGGCGTGCAGCTGGTCGCCTATGCGTCGGAAGAACCGCCCTACTTTCg                                                                                           <  1:2226299/100‑1 (MQ=255)
    ggCAGGATTACTCGAACTGGCACGTTTGTTACATCAGCAAGTACCGAAAACAGGCGTGCAGCTGGTCGCCTATGCGTCGGAAGAACCGCCCTACTTTCGt                                                                                          >  2:297321/1‑100 (MQ=255)
    ggCAGGATTACTCGAACTGGCACGTTTGTTACATCAGCAAGTACCGAAAACAGGCGTGCAGCTGGTCGCCTATGCGTCGGAAGAACCGCCCTACTTTCGt                                                                                          >  1:1958552/1‑100 (MQ=255)
    ggCAGGATTACTCGAACTGGCACGTTTGTTACATCAGCAAGTACCGAAAACAGGCGTGCAGCTGGTCGCCTATGCGTCGGAAGAACCGCCCTACTTTCGt                                                                                          >  2:1261616/1‑100 (MQ=255)
      cAGGATTATTCGAACTGGCACGTTTGTTACATCAGCAAGTACCGAAAACAGGCGTGCAGCTGGTCGCCTATGCGTCGGAAGAACCGCCCTACTTTCg                                                                                           <  2:2320886/97‑1 (MQ=255)
       aGGATTACTCGAACTGGCACGTTTGTTACATCAGCAAGTACCGAAAACAGGCGTGCAGCTGGTCGCCTATGCGTCGGAAGAACCGCCCTACTTTCGTAGc                                                                                       <  1:2885756/100‑1 (MQ=255)
       aGGATTACTCGAACTGGCACGTTTGTTACATCAGCAAGTACCGAAAACAGGCGTGCAGCTGGTCGCCTATGCGTCGGAAGAACCGCCCTACTTTCGTAGc                                                                                       <  1:297324/100‑1 (MQ=255)
       aGGATTACTCGAACTGGCACGTTTGTTACATCAGCAAGTACCGAAAACAGGCGTGCAGCTGGTCGCCTATGCGTCGGAAGAACCGCCCTACTTTCGTAGc                                                                                       <  1:336410/100‑1 (MQ=255)
       aGGATTACTCGAACTGGCACGTTTGTTACATCAGCAAGTACCGAAAACAGGCGTGCAGCTGGTCGCCTATGCGTCGGAAGAACCGCCCTACTTTCGTAGc                                                                                       >  2:1595399/1‑100 (MQ=255)
       aGGATTACTCGAACTGGCACGTTTGTTACATCAGCAAGTACCGAAAACAGGCGTGCAGCTGGTCGCCTATGCGTCGGAAGAACCGCCCTACTTTCGTAGc                                                                                       <  1:2109903/100‑1 (MQ=255)
       aGGATTACTCGAACTGGCACGTTTGTTACATCAGCAAGTACCGAAAACAGGCGTGCAGCTGGTCGCCTATGCGTCGGAAGAACCGCCCTACTTTCGTAGc                                                                                       <  1:1291114/100‑1 (MQ=255)
         gATTACTCGAACTGGCACGTTTGTTACATCAGCAAGTACCGAAAACAGGCGTTAAGATGGTCGCATATGCGTCAGAAGAACCGCCCTAATTTCGTAGCGa                                                                                     >  1:14791/1‑100 (MQ=255)
          aTTACTCGAACTGGCACGTTTGTTACATCAGCAAGTACCGAAAACAGGCGTGCAGCTGGTCGCCTATGCGTCGGAAGAACCGCCCTACTTTCGTAGCGAt                                                                                    <  2:2379138/100‑1 (MQ=255)
          aTTACTCGAACTGGCACGTTTGTTAAATCAGCAAGTACCGAAAACAGGCGTGAAGCTGGTCGCCTATGAGTCGGAAGAACCGCCCTAGTTTCGTAGCGAt                                                                                    >  2:2141238/1‑100 (MQ=255)
            tACTCGAACTGGCACGTTTGTTACATCAGCAAGTACCGAAAACAGGCGTGCAGCTGGTCGCCTATGCGTCGGAAGAACCGCCCTACTTTCGTAGCGATGa                                                                                  >  1:70526/1‑100 (MQ=255)
             aCTCGAACTGGCACGTTTGTTACATCAGCAAGTACCGAAAACAGGCGTGCAGCTGGTCGCCTATGCGTCGGAAGAACCGCCCTACTTTCGTAGCGATGaa                                                                                 >  1:260068/1‑100 (MQ=255)
             aCTCGAACTGGCACGTTTGTTACATCAGCAAGTACCGAAAACAGGCGTGCAGCTGGTCGCCTATGCGTCGGAAGAACCGCCCTACTTTCGTAGCGATGaa                                                                                 >  1:1173688/1‑100 (MQ=255)
              cTCGAACTGGCACGTTTGTTACATCAGCAAGTACCGAAAACAGGCGTGCAGCTGGTCGCCTATGCGTCGGAAGAACCGCCCTACTTTCGTAGCGATGaaa                                                                                <  1:1519281/100‑1 (MQ=255)
              cTCGAACTGGCACGTTTGTTACATCAGCAAGTACCGAAAACAGGCGTGCAGCTGGTCGCCTATGCGTCGGAAGAACCGCCCTACTTTCGTAGCGATGaaa                                                                                >  1:1080362/1‑100 (MQ=255)
              cTCGAACTGGCACGTTTGTTACATCAGCAAGTACCGAAAACAGGCGTGCAGCTGGTCGCCTATGCGTCGGAAGAACCGCCCTACTTTCGTAGCGATGaaa                                                                                >  1:2676048/1‑100 (MQ=255)
              cTCGAACTGGCACGTTTGTTACATCAGCAAGTACCGAAAACAGGCGTGCAGCTGGTCGCCTATGCGTCGGAAGAACCGCCCTACTTTCGTAGCGATGaaa                                                                                >  1:1262616/1‑100 (MQ=255)
               tCGAACTGGCACGTTTGTTACATCAGCAAGTACCGATAACTGGCGTGCAGCTGGTCGCCTATGCGTCGGAAGAACCGCCCTACTTTCGTAGCGATGAAAt                                                                               <  1:2307489/100‑1 (MQ=255)
                cGAAATTGCACGTTTGTTACATCAGCAAGTACCGAAAACAGGCGTGCAGCTGGTCGCCTATGCGTCGGAAGATCCGCCCTACTTTCCTAg                                                                                        <  2:2704800/90‑1 (MQ=255)
                     tGGCACGTTTGTTACATCAGCAAGTACCGAAAACAGGCGTGCAGCTGGTCGCCTATGCGTCGGAAGAACCGCCCTACTTTCGTAGCGATGAAATGGGGAg                                                                         <  1:21746/100‑1 (MQ=255)
                        cACGTTTGTTACATCAGCAAGTACCGAAAACAGGCGTGCAGCTGGTCGCCTATGCGTCGGAAGAACCGCCCTACTTTCGTAGCGATGAAATGGGGAgcgc                                                                      <  1:305081/100‑1 (MQ=255)
                        cACGTTTGTTACATCAGCAAGTACCGAAAACAGGCGTGCAGCTGGTCGCCTATGCGTCGGAAGAACCGCCCTACTTTCGTAGCGATGAAATGGGGAgcgc                                                                      <  2:230842/100‑1 (MQ=255)
                          cGTTTGTTACATCAGCAAGTACCGAAAACAGGCGTGCAGCTGGTCGCCTATGCGTCGGAAGAACCGCCCTTCTTTCGTAGCGATAAAATGGGGAGcgcg                                                                     >  1:1482485/1‑99 (MQ=255)
                               gTTACATCAGCAAGTACCGAAAACTGGCGTGCAGCTGGTCGCCTATGCGTCGGAAGAACCGCCCTACTTTCGTAGCGATGAAATGGGGAGCGCGGtgcat                                                               >  2:2174626/1‑100 (MQ=255)
                               gTTACATCAGCAAGTACCGAAAACAGGCGTGCAGCTGGTCGCCTATGCGTCGGAAGAACCGCTCTACTTTCGTAGCGAAGAAATGGGGAGCGCGGtgca                                                                >  2:799752/1‑99 (MQ=255)
                               gTTACATCAGCAAGTACCGAAAACAGGCGTGCAGCTGGTCGCCTATGCGTCGGAAGAACCGCCCTACTTTCGTAGCGATGAAATGGGGAGCGCGGtgcat                                                               >  1:170679/1‑100 (MQ=255)
                                 tACATCAGCAAGTATCGAAAACTGGCGTGTAGCTGGTCGCCTATGTGTCGGAAGAACCGCCCTACTTTCGTAGCGATGAAATGGGGAGCGCGGtgcatgc                                                             <  1:488105/100‑1 (MQ=255)
                                  aCATCAGCAAGTACCGAAAACAGGCGTGCAGCTGGTCGCCTATGCGTCGGAAGAACCGCCCTTCTTTCGTAGCGATGAAATGGGGAGCGCGGtgcatgca                                                            <  1:1800182/100‑1 (MQ=255)
                                  aCATCAGCAAGTACCGAAAACAGGCGTGCAGCTGGTCGCCTATGCGTCGGAAGAACCGCCCTTCTTTCGTAGCGATGAAATGGGGAGCGCGGtgcatgca                                                            <  2:2193639/100‑1 (MQ=255)
                                  aCATCAGCAAGTACCGAAAACAGGCGTGCAGCTGGTCGCCTATGCGTCGGAAGAACCGCCCTACTTTCGTAGCGATGAAATGGGGAGCGCGGtgcatgca                                                            >  2:2166050/1‑100 (MQ=255)
                                  aCATCAGCAAGTACCGAAAACAGGCGTGCAGCTGGTCGCCTATGCGTCGGAAGAACCGCCCTACTTTCGTAGCGATGAAATGGGGAGCGCGGtgcatgca                                                            >  1:6667/1‑100 (MQ=255)
                                  aCATCAGCAAGTACCGAAAACAGGCGTGCAGCTGGTCGCCTATGCGTCGGAAGAACCGCCCTACTTTCGTAGCGATGAAATGGGGAGCGCGGtgcatgca                                                            <  1:395740/100‑1 (MQ=255)
                                  aCATCAGCAAGTACCGAAAACAGGCGTGCAGCTGGTCGCCTATGCGTCGGAAGAACCGCCCTACTTTCGTAGCGATGAAATGGGGAGCGCGGtgcatgca                                                            >  1:2929131/1‑100 (MQ=255)
                                  aCATCAGCAAGTACCGAAAACAGGCGTGCAGCTGGTCGCCTATGCGTCGGAAGAACCGCCCTACTTTCGTAGCGATGAAATGGGGAGCGCGGtgcatgca                                                            <  2:3029862/100‑1 (MQ=255)
                                  aCATCAGCAAGTACCGAAAACAGGCGTGCAGCTGGTCCCCTATGCGTCGGAAGAACCGCCCTACTTTCGTAGCGATGAAATGGGGAGCGCGGtgcatgca                                                            >  2:1611278/1‑100 (MQ=255)
                                   cATCAGCAAGTACCGAAAACAGGCGTGCAGCTGGTCGCCTATGCGTCGGAAGAACCGCCCTACTTTCGTAGCGATGAAATGGGGAGCGCGGTGCATGCAg                                                           >  1:1354071/1‑100 (MQ=255)
                                   cATCAGCAAGTACCGAAAACAGGCGTGCAGCTGGTCGCCTATGCGTCGGAAGAACCGCCCTACTTTCGTAGCGATGAAATGGGGAGCGCGGTGCATGCAg                                                           >  2:1024139/1‑100 (MQ=255)
                                    aTCAGCAAGTACCGAAAACAGGCGTGCAGCTGGTCGCCTATGCGTCGGAAGAACCGCCCTACTTTCGTAGCGATGAAATGGGGAGCGCGGTGCATGCAGc                                                          <  2:1546442/100‑1 (MQ=255)
                                    aTCAGCAAGTACCGAAAACAGGCGTGCAGCTGGTCGCCTATGCGTCGGAAGAACCGCCCTACTTTCGTAGCGATGAAATGGGGAGCGCGGTGCATGCAGc                                                          <  1:1595418/100‑1 (MQ=255)
                                     tCAGCAAGTACCGAAAACAGGCGTGCAGCTGGTCGCCTATGCGTCGGAAGAACCGCCCTACTTTCGTAGCGATGAAATGGGGAGCGCGGTGCATGCAGCt                                                         >  2:2728645/1‑100 (MQ=255)
                                     tCAGCAAGTACCGAAAACAGGCGTGCAGCTGGTCGCCTATGCGTCGGAAGAACCGCCCTACTTTCGTAGCGATGAAATGGGGAGCGCGGTGCATGCAGCt                                                         >  2:2010818/1‑100 (MQ=255)
                                      cAGCAAGTACCGAAAACAGGCGTGCAGCTGGTCGCCTATGCGTCGGAAGAACCGCCCTACTTTCGTAGCGATGAAATGGGGAGCGCGGTGCATGCAGCtt                                                        >  2:1693497/1‑100 (MQ=255)
                                       aGCAAGTACCGAAAACAGGCGTGCAGCTGGTCGCCTATGCGTCGGAAGAACCGCCCTACTTTCGTAGCGATGAAATGGGGAGCGCGGTGCATGCAGCTTc                                                       >  1:65074/1‑100 (MQ=255)
                                        gCAAGTACCGAAAACAGGCGTGCAGCTGGTCGCCTATGCGTCGGAAGAACCGCCCTACTTTCGTAGCGATGAAATGGGGAGCGCGGTGCATGCAGCTTCg                                                      >  2:2105322/1‑100 (MQ=255)
                                          aaGTACCGAAAACAGGCGTGCAGCTGGTCGCCTATGCGTCGGAAGAACCGCCCTACTTTCGTAGCGATGAAATGGGGAGCGCGGTGCATGCAGCTTCGCt                                                    <  2:2216754/100‑1 (MQ=255)
                                           aGTACCGAAAACAGGCGTGCAGCTGGTCGCCTATGCGTCGGAAGAACCGCCCTACTTTCGTAGCGATGAAATGGGGAGCGCGGTGCATGCAGCTTCGCtt                                                   <  2:2988039/100‑1 (MQ=255)
                                            gTACCGAAAACAGGCGTGCAGCTGGTCGCCTATGCGTCGGAAGAACCGCCCTACTTTCGTAGCGATGAAATGGGGAGCGCGGTGCATGCAGCTTCGCTTg                                                  >  2:200186/1‑100 (MQ=255)
                                            gTACCGAAAACAGGCGTGCAGCTGGTCGCCTATGCGTCGGAAGAACCGCCCTACTTTCGTAGCGATGAAATGGGGAGCGCGGTGCATGCAGCTTCGCTTg                                                  >  1:2161970/1‑100 (MQ=255)
                                              aCCGAAAACAGGCGTGCAGCTGGTCGCTTATGCGTCGGAAGAACCGCCCTACTTTCGTAGCGATGAAATGGGGAGCGCGGTGCATGCAGCTTCGCTTGAg                                                <  1:162038/100‑1 (MQ=255)
                                              aCCGAAAACAGGCGTGCAGCTGGTCGCCTATGCGTCGGAAGAACCGCCCTACTTTCGTAGCGATGAAATGGGGAGCGCGGTGCATGCAGCTTCGCTTGAg                                                <  1:2600773/100‑1 (MQ=255)
                                               cgacgcataGGCGACCAGCTGCACGCCTATGCGTCGGAAGAACCGCCCTACTTTCGTAGCGATGAAATGGGGAGCGCGGTGCATGCAGCTTCGCTTGAg                                                >  2:66774/9‑99 (MQ=37)
                                               ccGAAAACAGGCGTGCAGCTGGTCGCCTATGCGTCGGAAGAACCGCCCTACTTTCGTAGCGATGAAATGGGGAGCGCGGTGCATGCAGCTTCGCTTGAg                                                <  1:2058434/99‑1 (MQ=255)
                                               ccGAAAACAGGCGTGCAGCTGGTCGCCTATGCGTCGGAAGAACCGCCCTACTTTCGTAGCGATGAAATGGGGAGCGCGGTGCATGCAGCTTCGCTTGAGc                                               >  2:2375646/1‑100 (MQ=255)
                                                 gAAAACAGGCGTGCAGCTGGTCGCCTATGCGTCGGAAGAACCGCCCTACTTTCGTAGCGATGAAATGGGGAGCGCGGTGCATGCAGCTTCGCTTGAGCGt                                             >  1:807588/1‑100 (MQ=255)
                                                   aaaCAGGCGTGCAGCTGGTCGCCTATGCGTCGGAAGAACCGCCCTACTTTCGTAGCGATGAAATGGGGAGCGCGGTGCATGCAGCTTCGCTTGAGCGTcc                                           <  1:1080277/100‑1 (MQ=255)
                                                    aaCAGGCGTGCCGCTGGTCGCCTATGCGTCGGAAGAACCGCCCTACTGTCGTAGGGATGAAACGGGGAGCGCGGTGCATGCAGCTTCGCtt                                                   >  2:2314610/1‑91 (MQ=255)
                                                    aaCAGGCGTGCAGCTGGTCGCCTATGCGTCGGAAGAACCGCCCTACTTTCGTAGCGATGAAATGGGGAGCGCGGTGCATGCAGCTTCGCTTGAGCGTCCa                                          <  2:2285942/100‑1 (MQ=255)
                                                      cAGGCGTGCAGCTGGTCGCCTATGCGTCGGAAGAACCGCCCTACTTTCGTAGCGATGAAATGGGGAGCGCGGTGCATGCAGCTTCGCTTGAGCGTCCAGt                                        <  2:1173677/100‑1 (MQ=255)
                                                       aGGCGTGCAGCTGGTCGCCTATGCGTCGGAAGAACCGCCCTACTTTCGTAGCGATGAAATGGGGAGCGCGGTGCATGCAGCTTCGCTTGAGCGTCCAGTg                                       <  1:1628358/100‑1 (MQ=255)
                                                       aGGCGTGCAGCTGGTCGCCTATGCGTCGGAAGAACCGCCCTACTTTCGTAGCGATGAAATGGGGAGCGCGGTGCATGCAGCTTCGCTTGAGCGTCCAGTg                                       <  1:850920/100‑1 (MQ=255)
                                                       aGGCGTGCAGCTGGTCGCCTATGCGTCGGAAGAACCGCCCTACTTTCGTAGCGATGAAATGGGGAGCGCGGTGCATGCAGCTTCGCTTGAGCGTCCAGTg                                       <  1:329385/100‑1 (MQ=255)
                                                           gTGCAGCTGGTCGCCTATGCGTCGGAATAACCGCCCTACTTTCGTAGCGATGAAATGGGGAGCGCGGTGCATGCAGCTTCGCTTGAGCGTCCAGTGAAAt                                   <  1:2356596/100‑1 (MQ=255)
                                                           gTGCAGCTGGTCGCCTATGCGTCGGAAGAACCGCCCTACTTTCGTAGCGATGAAATGGGGAGCGCGGTGCATGCAGCTTCGCTTGAGCGTCCAGTGAAAt                                   >  2:1197042/1‑100 (MQ=255)
                                                            tGCAGCTGGTCGCCTATGCGTCGGAAGAACCGCCCTACTTTCGTAGCGATGAAATGGGGAGCGCGGTGCATGCAGCTTCGCTTGAGCGTCCAGTGAAAtt                                  <  2:361481/100‑1 (MQ=255)
                                                             gCAGCTGGTCGCCTATGCGTCGGAAGAACCGCCCTACTTTCGTAGCGATGAAATGGGGAGCGCGGTGCATGCAGCTTCGCTTGAGCGTCCAGTGAAATTa                                 >  1:1779344/1‑100 (MQ=255)
                                                             gCAGCTGGTCGCCTATGCGTCGGAAGAACCGCCCTACTTTCGTAGCGATGAAATGGGGAGCGCGGTGCATGCAGCTTCGCTTGAGCGTCCAGTGAAATTa                                 >  1:477401/1‑100 (MQ=255)
                                                             gCAGCTGGTCGCCTATGCGTCGGAAGAACCGCCCTACTTTCGTAGCGATGAAATGGGGAGCGCGGTGCATGCAGCTTCGCTTGAGCGTCCAGTGAAATTa                                 >  1:1833943/1‑100 (MQ=255)
                                                               aGCTGGTCGCCTATGCGTCGGAAGAACCGCCCTACTTTCGTAGCGATGAAATGGGGAGCGCGGTGCATGCAGCTTCGCTTGAGCGTCCAGTGAAATTAAt                               <  2:1958529/100‑1 (MQ=255)
                                                               aGCTGGTCGCCTATGCGTCGGAAGAACCGCCCTACTTTCGTAGCGATGAAATGGGGAGCGCGGTGCATGCAGCTTCGCTTGAGCGTCCAGTGAAATTAAt                               <  1:2105345/100‑1 (MQ=255)
                                                               aGCTGGTCGCCTATGCGTCGGAAGAACCGCCCTACTTTCGTAGCGATGAAATGGGGAGCGCGGTGCATGCAGCTTCGCTTGAGCGTCCAGTGAAATTAAt                               <  1:2630789/100‑1 (MQ=255)
                                                                gCTGGTCGCCTATGCGTCTGAAGAACTGCTCTACTTTCTTATCGATGAAATGGTGAGCGCGGTGCAGGCAGCTTCGCTTGAGCGTCCAGTGAAATTAATg                              <  1:852511/100‑1 (MQ=255)
                                                                gCTGGTCGCCTATGCGTCGGAAGAACCGCCCTACTTTCGTAGCGATGAAATGGGGAGCGCGGTGCATGCAGCTTCGCTTGAGCGTCCAGTGAAATTAATg                              <  2:1835992/100‑1 (MQ=255)
                                                                 cTGGTCGCCTATGCGTCGGAAGAACCGCCCTACTTTCGTAGCGATGAAATGGGGAGCGCGGTGCATGCAGCTTCGCTTGAGCGTCCAGTGAAATTAATGa                             <  1:1298396/100‑1 (MQ=255)
                                                                   ggTCGCCTATGCGTCGGAAGAACCGCCCTACTTTCGTAGCGATGAAATGGGGAGAGCGGTGCATGCAGCTTCGCTTGAGCGTCCAGTGAAATTAATGATa                           >  1:2469868/1‑100 (MQ=255)
                                                                      cGCCTATGCGTCGGAAGAACCGCCCTACTTTCGTAGCGATGAAATGGGGAGCGCGGTGCATGCAGCTTCGCTTGAGCGTCCAGTGAAATTAATGATAGCa                        >  1:1145348/1‑100 (MQ=255)
                                                                       gCCTATGCGTCGGAAGAACCGCCCTACTTTCGTAGCGATGAAATGGGGAGCGCGGTGCATGCAACTTCGCTTGAGCGTCCAGTGAAATTAATGATAGCAc                       >  1:599597/1‑100 (MQ=255)
                                                                        ccTATGCGTCGGAAGAACCGCCCTACTTTCGTAGCGATGAAATGGGGAGCGCGGTGCATGCAGCTTCGCTTGAGCGTCCAGTGAAATTAATGATAGCACt                      >  1:425382/1‑100 (MQ=255)
                                                                        ccTATGCGTCGGAAGAACCGCCCTACTTTCGTAGCGATGAAATGGGGAGCGCGGTGCATGCAGCTTCGCTTGAGCGTCCAGTGAAATTAATGATAGCACt                      >  2:2385822/1‑100 (MQ=255)
                                                                          tATGCGTCGGAAGAACCGCCCTACTTTCGTAGCGATGAAATGGGGAGCGCGGTGCATGCAGCTTCGCTTGAGCGTCCAGTGAAATTAATGATAGCACTgg                    >  2:2926711/1‑100 (MQ=255)
                                                                          tATGCGTCGGAAGAACCGCCCTACTTTCGTAGCGATGAAATGGGGAGCGCGGTGCATGCAGCTTCGCTTGAGCGTCCAGTGAAATTAATGATAGCACTgg                    <  2:2749477/100‑1 (MQ=255)
                                                                           aggCGTCGGAAGAACCGCCCTACTTTCGTAGCGATGAAATGGGGAGCGCGGTGCATGCAGCTTCGCTTGAGCGTCCAGTGAAATTAATGATAGCACTGga                   <  2:1733380/98‑1 (MQ=255)
                                                                             gCGTCGGAAGAACCGCCCTACTTTCGTAGCGATGAAATGGGGAGCGCGGTGCATGCAGCTTCGCTTGAGCGTCCAGTGAAATTAATGATAGCACTGgaga                 <  1:2012565/100‑1 (MQ=255)
                                                                              cGTCGGAAGAACCGCCCTACTTTCGTAGCGATGAAATGGGGAGCGCGGTGCATGCAGCTTCGCTTGAGCGTCCAGTGAAATTAATGATAGCACTGGAgat                >  2:1735546/1‑100 (MQ=255)
                                                                               gTCGGAAGAACCGCCCTACTTTCGTAGCGATGAAATGGGGAGCGCGGTGCATGCAGCTTCGCTTGAGCGTCCAGTGAAATTAATGATAGCACTGGAgatg               >  1:895594/1‑100 (MQ=255)
                                                                                  ggAAGAAGCGCCCGACTTTCGTAGCGATGAAATGGGGAGCGCAGTGCATGCAGCTTCGCTTGCGCGTCCAGTGAAATAAATGATAGCACTGGAGATGAtt            >  1:2054417/1‑100 (MQ=255)
                                                                                  ggAAGAACCGCCCTACTTTCGTAGCGATGAAATGGGGAGCGCGGTGCATGCAGCTTCGCTTGAGCGTCCAGTGAAATTAATGATAGCACTGGAGATGAtt            >  1:1127748/1‑100 (MQ=255)
                                                                                  ggAAGAACCGCCCTACTTTCGTAGCGATGAAATGGGGAGCGCGGTGCATGCAGCTTCGCTTGAGCGTCCAGTGAAATTAATGATAGCACTGGAGATGAtt            >  2:675364/1‑100 (MQ=255)
                                                                                     agaaCCGCCCTACTTTCGTAGCGATGAAATGGGGAGCGCGGTGCATGCAGCTTCGCTTGAGTGTCCAGTGAAATTAATGATAGCACTGGAGATGATTGGc         <  2:1874920/100‑1 (MQ=255)
                                                                                     agaaCCGCCCTACTTTCGTAGCGATGAAATGGGGAGCGCGGTGCATGCAGCTTCGCTTGAGCGTCCAGTGAAATTAATGATAGCACTGGAGATGATTGGc         <  1:2913954/100‑1 (MQ=255)
                                                                                      gaaCCGCCCTACTTTCGTAGCGATGAAATGGGGAGCGCGGTGCATGCAGCTTCGCTGGAGCGTCCAGTGAAATTAATGATAGCACTGGGGATGATTGGc         >  2:2578938/1‑99 (MQ=255)
                                                                                        aCCGCCCTACTTTCGTAGCGATGAAATGGGGAGCGCGGTGCATGCAGCTTCGCTTGAGCGTCCAGTGAAATTAATGATAGCACTGGAGATGATTGGCTAt      >  2:777439/1‑100 (MQ=255)
                                                                                         ccGCCCTACTTTCGTAGCGATGAAATGGGGAGCGCGGTGCATGCAGCTTCGCTTGAGCGTCCAGTGAAATTAATGATAGCACTGGAGATGATTGGCTAtt     >  1:610955/1‑100 (MQ=255)
                                                                                          cGCCCTACTTTCGTAGCGATGAAATGGGGAGCGCGGTGCATGCAGCTTCGCTTGAGCGTCCAGTGAAATTAATGATAGCACTGGAGATGATTGGCTATTa    <  2:2421639/100‑1 (MQ=255)
                                                                                          cGCCCTACTTTCGTAGCGATGAAATGGGGAGCGCGGTGCATGCAGCTTCGCTTGAGCGTCCAGTGAAATTAATGATAGCACTGGAGATGATTGGCTATTa    <  1:1405620/100‑1 (MQ=255)
                                                                                            cccTACTTTCGTAGCGATGAAATGGGGAGCGCGGTGCATGCAGCTTCGCTTGAGCGTCCAGTGAAATTAATGATAGCACTGGAGATGATTGGCTATTACg  >  1:149423/1‑100 (MQ=255)
                                                                                                |                                                                                               
GTGTGGCAGGATTACTCGAACTGGCACGTTTGTTACATCAGCAAGTACCGAAAACAGGCGTGCAGCTGGTCGCCTATGCGTCGGAAGAACCGCCCTTCTTTCGTAGCGATGAAATGGGGAGCGCGGTGCATGCAGCTTCGCTTGAGCGTCCAGTGAAATTAATGATAGCACTGGAGATGATTGGCTATTACG  >  NC_000913/2386269‑2386460

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: