| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 2,495,173 | T→A | 100% | A40A (GCA→GCT) | yfdY ← | DUF2545 family putative inner membrane protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,495,173 | 0 | T | A | 97.2% | 265.4 / ‑1.6 | 85 | A40A (GCA→GCT) | yfdY | DUF2545 family putative inner membrane protein |
| Reads supporting (aligned to +/- strand): ref base T (1/2); new base A (48/34); total (49/36) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.71e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.73e-01 | |||||||||||
AGCGAATCATCAAGCCACAAGCCGGGGCAAGAAACATAAATATCCCCGTCACTAGTTCATTACCGCCAGTTAACCACATGGTGAAGTAATAAATGAGTGCAGCGAGGATCACCATGCCGAAGAAAGAAGAGACCGCCGATACCACGTTCAGTACCTGACCGATGTAGCCGCTTACGCAGACAATACACAGGGCG > NC_000913/2495076‑2495269 | aGCGAATCATCAAGCCACAAGCCGGGGCAAGAAACATAAATATCCCCGTCACTAGTTCATTAACGCCAGTTAACCACATGATGAAGTAATAAATGAGAGc > 1:950998/1‑100 (MQ=255) gCGAATCATCAAGCCACAAGCCGGGGCAAGAAACATAAATATCCCCGTCACTAGTTCATTACCGCCAGTTAACCACATGGTGAAGTAATAAATGAGAGCa > 2:702970/1‑100 (MQ=255) gCGAATCATCAAGACACAAGCCGGGGCAAGAAACATAATTATCCCCGTCACTAGTTCATTACCGCCAGTTAACCACATGGTGAAGTAATAAATGAGAGCa > 2:1869938/1‑100 (MQ=255) gAATCATCAAGCCACAAGCCGGGGCAAGAAACATAAATATCCCCGTCACTAGTTCATTACCGCCAGTTAACCACATGGTGAAGTAATAAATGAGagcagc > 2:2427554/1‑100 (MQ=255) aaTCATCAAGCCACAAGCCGGGGCAAGAAACATAAATATCCCCGTCACTAGTTCATTACCGCCAGTTAACCACATGGTGAAGTAATAAATGAGTGCAGCg < 2:2374875/100‑1 (MQ=255) tcatcaAGCCACAAGCCGGGGCAAGAAACATAAATATCCCCGTCACTAGTTCATTACCGCCAGTTAACCACATGGTGAAGTAATAAATGAGAGCAGCGAg < 2:74829/100‑1 (MQ=255) catcaAGCCACAAGCCGGGGCAAGAAACATAAATATCCCCGTCACTAGTTCATTACCGCCAGTTAACCACATGGTGAAGTAATAAATGAGAGCAGCGAgg > 2:2965944/1‑100 (MQ=255) tcaAGCCACAAGCCGGGGCAAGAAACATAAATATCCCCGTCACTAGTTCATTACCGCCAGTTAACCACATGGTGAAGTAATAAATGAGAGCAGCGAGGa > 2:558365/1‑99 (MQ=255) tcaAGCCACAAGCCGGGGCAAGAAACATAAATATCCCCGTCACTAGTTCATTACCGCCAGTTAACCACATGGTGAAGTAATAAATGAGAGCAGCGAGGAt < 1:900158/100‑1 (MQ=255) caAGTCACAAGCCGGGGCAAGAAACATAAATATCCCCGTCACTAGTTCATTACCGCCAGTTAACCACATGGTGAAGTAATAAATGAGAGCAGCGAGGATc > 2:2913469/1‑100 (MQ=255) caAGCCACAAGCCGGGGCAAGAAACATAAATATCCCCGTCACTAGTTCATTACCGCCAGTTAACCACATGGTGAAGTAATAAATGAGAGCAGCGAGGATc > 1:482344/1‑100 (MQ=255) aaGCCACAAGCCGGGGCAAGAAACATAAATATCCCCGTCACTAGTTCATTACCGCCAGTTAACCACATGGTGAAGTAATAAATGAGAGCAGCGAGGATCa < 2:2779127/100‑1 (MQ=255) aGCCACAAGCCGGGGCAAGAAACATAAATATCCCCGTCACTAGTTCATTACCGCCAGTTAACCACATGGTGAAGTAATAAATGAGAGCAGCGAGGATCAc > 1:1955396/1‑100 (MQ=255) aGCCACAAGCCGGGGCAAGAAACATAAATATCCCCGTCACTAGTTCATTACCGCCAGTTAACCACATGGTGAAGTAATAAATGAGAGCAGCGAGGATCAc < 1:2917346/100‑1 (MQ=255) aGCCACAAGCCGGGGCAAGAAACATAAATATCCCCGTCACTAGTTCATTACCGCCAGTTAACCACATGGTGAAGTAATAAATGAGAGCAGCGAGGATCAc > 2:1796091/1‑100 (MQ=255) aGCCACAAGCCGGGGCAAGAAACATAAATATCCCCGTCACTAGTTCATTACAGCCAGTTAACCACATGGTGAAGTAATAAATGAGAGCAGCGAGGATCAc > 2:272995/1‑100 (MQ=255) gCCACAAGCCGGGGCAAGAAACATAAATATCCCCGTCACTAGTTCATTACCGCCAGTTAACCACATGGTGAAGTAATAAATGAGAGCAGCGAGGATCAcc > 2:879543/1‑100 (MQ=255) cacaAGCCGGGGCAAGAAACATAAATATCCCCGTCACTAGTTCATTACCGCCAGTTAACCACATGGTGAAGTAATAAATGAGAGCAGCGAGGATCACCAt > 1:1466403/1‑100 (MQ=255) aaGCCGGGGCAAGAAACATAAATATCCCCGTCACTAGTTCATTACCGCCAGTTAACCACATGGTGAAGTAATAAATGAGTGCAGCGAGGATCACCATGcc > 2:2462143/1‑100 (MQ=255) aaGCCGGGGCAAGAAACATAAATATCCCCGTCACTAGTTCATTACCGCCAGTTAACCACATGGTGAAGTAATAAATGAGAGCAGCGAGGATCACCATGcc < 2:99293/100‑1 (MQ=255) aaGCCGGGGCAAGAAACATAAATATCCCCGTCACTAGTTCATTACCGCCAGTTAACCACATGGTGAAGTAATAAATGAGAGCAGCGAGGATCACCATGcc < 1:2678249/100‑1 (MQ=255) aaGCCGGGGCAAGAAACATAAATATCCCCGTCACTAGTTCATTACCGCCAGTTAACCACATGGTGAAGTAATAAATGAGAGCAGCGAGGATCACCATGcc < 2:1196912/100‑1 (MQ=255) aaGCCGGGGCAAGAAACATAAATATCCCCGTCACTAGTTCATTACCGCCAGTTAACCACATGGTGAAGTAATAAATGAGAGCAGCGAAGATcggaagagc > 1:2366904/1‑92 (MQ=255) aGCCGGGGCAAGAAACATAAATATTCCCGTCACTAGTTCATTAACGCCAGTTAACCACATGGTGAAGTAATAAATGAGAGCAGCGAGGATCACCATGCCg < 1:410150/100‑1 (MQ=255) aGCCGGGGCAAGAAACATAAATATCCCCGTCACTAGTTCATTACCGCCAGTTAACCACATGGTGAAGTAATAAATGAGAGCAGCGAGGATCACCATGCCg > 1:1813368/1‑100 (MQ=255) gCCGGGGCAAGACACATAAATATCCCCGTCACTAGTTCATTACCGCCAGTTAACCACATGGCGAAGTAATAAATGAGAGCAGCAAGGATCACCATGCCga > 2:1770755/1‑100 (MQ=255) gCCGGGGCAAGAAACATATATATCCCCGTCACTAGTTTATTACCGCCAGTTAACCACATGGTGAAGTAATAAATGAGAGCAGCGAGGATCACCATGCCga < 1:87181/100‑1 (MQ=255) gCCGGGGCAAGAAACATAAATATCCCCGTCACTAGTTCATTACCGCCAGTTAACCACATGGTGAAGTAATAAATGAGAGCAGCGAGGATCACCATGCCga > 2:1746070/1‑100 (MQ=255) gCAAGAAAGATAAATATCCCCGTCACTAGTTCATTACCGCCAGTTAACCAGATGGTGAAGTAATAAATGAGAGCAGCGAGGATCACCATGCCGaagaaag < 2:862541/100‑1 (MQ=255) gCAAGAAACATAAATATCCCCGTCACTAGTTCATTACCGCCAGTTAACCACATGGTGAAGTAATAAATGAGAGCAGCGAGGATCACCATGCCGaagaaag > 1:714740/1‑100 (MQ=255) cAAGAAACATAAATATCCCCGTCACTAGTTCATTACCGCCAGTTAACCACATGGTGAAGTAATAAATGAGAGCAGCGAGGATCACCATGCCGaagaaaga > 1:962891/1‑100 (MQ=255) cAAGAAACATAAATATCCCCGTCACTAGTTCATTACCGCCAGTTAACCACATGGTGAAGTAATAAATGAGAGCAGCGAGGATCACCATGCCGaagaaaga > 1:1623363/1‑100 (MQ=255) gAAACATAAATATCACCATCACTAATTCATTAACACCAGTTAAACACATGGAAAAGTAATAAATGAGAGCAGCGAAGATCACCATACCGAAGAAagaaga > 2:5815/1‑100 (MQ=255) aaaCATAAATATCCCCGTCACTAGTTCATTACCGCCAGTTAACCACATGGTGAAGTAATAAATGAGAGCAGCGAGGATCACCATGCCGAAGAAAGAagag < 2:2327894/100‑1 (MQ=255) aaaCATAAATATCCCCGTCACTAGTTCATTACCGCCAGTTAACCACATGGTGAAGTAATAAATGAGAGCAGCGAGGATCACCATGCCGAAGAAAGAagag < 2:296168/100‑1 (MQ=255) cATAAATATCCCCGTCACTAGTTCATTACCGCCAGTTAACAACATGGAGAAGTAATAAATGAGAGAAGCAAGGATCACCATGCCGAATAAAGAAGAGAcc > 2:1100696/1‑100 (MQ=255) aTAAATATCCCCGTCACTAGTTCATTACCGCCAGTTAACCACATGGTGAAGTAATAAATGAGAGCAGCGAGGATCACCATGCCGAAGAAAGAAGAGAccg > 2:259815/1‑100 (MQ=255) aTAAATATCCCCGTCACTAGTTCATTACCGCCAGTTAACCACATGGTGAAGTAATAAATGAGAGCAGCGAGGATCACCATGCCGAAGAAAGAAGAGAccg > 1:856740/1‑100 (MQ=255) tAAATATCCCCGTCACTAGTTCATTACCGCCAGTTAACCACATGGTGAAGTAATAAATGAGAGCAGCGAGGATCACCATGCCGAAGAAAGAAGAGAccgc > 1:1455590/1‑100 (MQ=255) cgatctcccGTCACTAGTTCATTACCGCCAGTTAACCACATGGTGAAGTAATAAATGAGAGCAGCGAGGATCACCATGCCGAAGAAAGAAGAGAccgccg < 2:2413903/94‑1 (MQ=255) atatCCCCGTCACTAGTTCATTACCGCCAGTTAACCACATGGTGAAGTAATAAATGAGAGCAGCGAGGATCACCATGCCGAAGAAAGAAGAGACCGCCGa > 1:266277/1‑100 (MQ=255) tatCCCCGTCACTAGTTCATTACCGCCAGTTAACCACATGGTGAAGTAATAAATGAGAGCAGCGAGGATCACCATGCCGAAGAAAGAAGAGACCGCCGAt > 2:1712177/1‑100 (MQ=255) atCCCCGTCACTAGTTCATTACCGCCAGTTAACCACATGGTGAAGTAATAAATGAGAGCATCGAGGATCACCATGCCGAAGAAAAAAGAGACCGCAGATa > 1:461837/1‑100 (MQ=255) ccccGTCACTAGTTCATTACCGCCAGTTAACCACATGGTGAAGTAATAAATGAGAGCAGCGAGGATCACCATGCCGAAGAAAGAAGAGACCGCCGATAcc > 2:713075/1‑100 (MQ=255) cccGTCACTAGTTCATTACCGTCAGTTAACCACATGGTGAAGTAATAAATGAGAGCAGCGAGGATCACCATGCCGAAGAAAGAAGAGACCGCCGATACCa < 1:520963/100‑1 (MQ=255) cccGTCACTAGTTCATTACCGCCAGTTAACCACATGGTTAAGTAATAAATGAGAGCAGCGAGGATCACCATGCTGAAGTAAGAAGAGACCGCCGATa < 2:644045/97‑1 (MQ=255) cccGTCACTAGTTCATTACCGCCAGTTAACCACATGGTGAAGTAATAAATGAGAGCAGCGAGGATCACCATGCCGAAGAAAGAAGAGACCGCCGagatcg > 1:2413932/1‑95 (MQ=255) cACTAGTTCATTACCGCCAGTTAACCACATGGTGAAGTAATAAATGAGAGCAGCGAGGATCACCATGCCGAAGAAAGAAGAGACCGCCGATACCACGTTc > 2:2625028/1‑100 (MQ=255) cACTAGTTCATTACCGCCAGTTAACCACATGGTGAAGTAATAAATGAGAGCAGCGAGGATCACCATGCCGAAGAAAGAAGAGACCGCCGATACCACGTTc < 1:1770965/100‑1 (MQ=255) cTAGTTCATTACCGCCAGTTAACCACATGGTGAAGTAATAAATGAGAGCAGCGAGGATCACCATGCCGAAGAAAGAAGAGACCGCCGATACCACGTTCAg > 1:10708/1‑100 (MQ=255) aGTTCATTACCGCCAGTTAACCACATGGTGAAGTAATAAATGAGAGCAGCGAGGATCACCATGCCGAAGAAAGAAGAGACCGCCGATACCACGTTCAGTa > 2:2556798/1‑100 (MQ=255) aGTTCATTACCGCCAGTTAACCACATGATTAAGTAATAAATAAGAGCAGCGAGTATCACCATGCCGAATAAAGAAGATACCGCCGATACCACTTTCAGt < 2:1412184/99‑1 (MQ=255) gTTCATTACCGCCAGTTAACCACATGGTGAAGTAATAAATGAGAGCAGCGAGGATCACCATGCCGAATAAAGAAGATACCGCCTATACCACGTTCAGTAc < 2:2137310/100‑1 (MQ=255) ttcacgtccGCCAGTGAACCACATGGTGAAGTAAGAAGGGAGTGCCGCGTTGATCACCATGCCGACGAAAGAAGAGTCCGCCGATACCGCGttga < 2:1739854/88‑3 (MQ=255) tgcATTACCGCTAGTTAACCACATGGTTAAGTAATAAATGAGAGCAGCGAGGATCACCATGCCGAAGAAAGAAGAGACCGCCGATACCACGTTCAGTAcc < 1:1234646/98‑1 (MQ=255) cATTACCGCCAGTTAACCACATGGTGAAGTAATAAATGAGAGCAGCGAGGATCACCATGCCGAAGAAAGAAGAGACCGCCGATACCACGTTCAGTACCTg < 1:2914939/100‑1 (MQ=255) cATTACCGCCAGTTAACCACATGGTGAAGTAATAAATGAGAGCAGCGAGGATCACCATGCCGAAGAAAGAAGAGACCGCCGATACCACGTTCAGTACCTg < 2:1980507/100‑1 (MQ=255) ttACCGCCAGTTAACCACATGGTGAAGTAATAAATGAGAGCAGCGAGGATCACCATTCCGAAGAAAGAAGAGACCTCCGATACCACGTTCAGTACCTGAc < 2:2454483/100‑1 (MQ=255) ttACCGCCAGTTAACCACATGGTGAAGTAATAAATGAGAGCAGCGAGGATCACCATGCCGAAGAAAGAAGAGACCGCCGATACCACGTTCAGTACCTGAc > 2:2980981/1‑100 (MQ=255) aCCGCCAGTTAACCACATGGTGAAGTAATAAATGAGAGCAGCGAGGATCACCATGCCGAAGAAAGAAGAGACCGCCGATACCACGTTCAGTACCTGACCg < 1:2663016/100‑1 (MQ=255) cGCCAGTTAACCACATGGTGAAGTAATAAATGAGAGCAGCGAGGATCACCATGCCGAAGAAAGAAGAAACCGCCGATACCACGTTCAGTACCTGACCGAt > 1:195368/1‑100 (MQ=255) gctaGTTAACCACATGGTGAAGTAATAAATGAGAGCAGCGAGGATCACCATGCCGAAGAAAGAAGAGACCGCCGATACCACGTTCAGTACCTGACCGATg < 2:2034364/97‑1 (MQ=255) gCCAGTTAACCACATGGTGAAGTAATAAATGAGAGCAGCGAGGATCACCATGCCGAAGAAAGAAGAGACCGCCGATACCACGTTCAGTACCTGACCGATg < 2:206370/100‑1 (MQ=255) ccAGTTAACCACATGGTGAAGTAATAAATGAGAGCAGCGAGGATCACCATGCCGAAGAAAGAAGAGACCGCCGATACCACGTTCAGTACCTGACCGATGt > 1:2603470/1‑100 (MQ=255) cAGTTAACCACATGGTGAAGTAATAAATGAGAGCAGCGAGGATCACCATGCCGAAGAAAGAAGAGACCGCCGATACCACGTTCAGTACCTGACCGATGTa < 2:1955373/100‑1 (MQ=255) cAGTTAACCACATGGTGAAGTAATAAATGAGAGCAGCGAGGATCACCATGCCGAAGAAAGAAGAGACCGCCGATACCACGTTCAGTACCTGACCGATGTa > 1:2386873/1‑100 (MQ=255) tAACCACATGGTGAAGTAATAAATGAGAGCAGCGAGGATCACCATGCCGAAGAAAGAAGAGACCGCCGATACCACGTTCAGTACCTGACCGATGTAGCCg > 1:1075105/1‑100 (MQ=255) aaCCACATGGTGAAGTAATAAATGAGAGCAGCGAGGATCACCATGCCGAAGAAAGAAGAGACCGCCGATACCACGTTCAGTACCTGACCGATGTAGCCg > 2:471709/1‑99 (MQ=255) aCCACATGGTGAAGTAATAAATGAGAGCAGCGAGGATCACCATGCCGAAGAAAGAAGAGACCGCCGATACCACGTTCAGTACCTGACCGATGTAGCCGCt < 2:2738152/100‑1 (MQ=255) ccACATGGTGAAGTAATAAATGAGAGCAGCGATGATCACCATGCCGAAGAAAGAAGAGACCGCCGATACCACGTTCAGTACCTGACCGATGTAGCCGCt < 1:490297/99‑1 (MQ=255) ccACATGGTGAAGTAATAAATGAGAGCAGCGAGGATCACCATGCCGAAGAAAGAAGAGACCGCCGATACCACGTTCAGTACCTGACCGATGTAGCCGCtt > 1:1200756/1‑100 (MQ=255) cacaTGGTGAAGTAATAAATGAGAGCAGCGAGGATCACCATGCCGAAGAAAGAAGAGACCGCCGATACCACGTTCAGTACCTGACCGATGTAGCCGCTTa > 1:3009459/1‑100 (MQ=255) cacaTGGTGAAGTAATAAATGAGAGCAGCGAGGATCACCATGCCGAAGAAAGAAGAGACCGCCGATACCACGTTCAGTACCTGACCGATGTAGCAGCTTa > 2:1736691/1‑100 (MQ=255) ggTGAAGTAATAAATGAGAGCAGCGAGGATCACCATGCCGAAGAAAGAAGAGACCGCCGATACCACGTTCAGTACCTGACCGATGTAGCCGCTTACGCAg < 2:1517294/100‑1 (MQ=255) gTGAAGTAATAAATGAGAGCAGCGAGGATCACCATGCCGAAGAAAGAAGAGACCGCCGATACCACGTTCAGTACCTGACCGATGTAGCCGCTTACGCAGa < 1:2965977/100‑1 (MQ=255) tGAAGTAATAAATGAGAGCAGCGAGGATCACCATGCCGAAGAAAGAAGAGACCGCCGATACCACGTTCAGTACCTGCCCGATGTAGCCGATTACGCa < 2:2578934/97‑1 (MQ=255) tGAAGTAATAAATGAGAGCAGCGAGGATCACCATGCCGAAGAAAGAAGAGACCGCCGATACCACGTTCAGTACCTGACCGATGTAGCCGCTTACGCAGAc < 1:308549/100‑1 (MQ=255) tGAAGTAATAAATGAGAGCAGCGAGGATCACCATGCCGAAGAAAGAAGAGACCGCCGATACCACGTTCAGTACCTGACCGATGTAGCCGCTTACGCAGAc < 1:305463/100‑1 (MQ=255) aGTAATAAATGAGAGCAGCGAGGATCACCATGCCGAAGAAAGAAGAGACCGCCGATACCACGTTCAGTACCTGACCGATGTAGCCGCTTACGCAGACAAt > 1:1354952/1‑100 (MQ=255) taataaATGAGAGCAGCGAGGATCACCATGCCGAAGAAAGAAGAGACCGCCGATACCACGTTCAGTACCTGACCGATGTAGCCGCTTACGCAGACAATac < 1:800288/100‑1 (MQ=255) taataaATGAGAGCAGCGAGGATCACCATGCCGAAGAAAGAAGAGACCGCCGATACCACGTTCAGTACCTGACCGATGTAGCCGCTTACGCAGACAATac > 2:879498/1‑100 (MQ=255) aataaATGAGAGCAGCGAGGATCACCATGCCGAAGAAAGAAGAGACCGCCGATACCACGTTCAGTACCTGACCGATGTAGCCGCTAACGCAGACAATaca > 1:212738/1‑100 (MQ=255) aaaTGAGAGCAGCGAGGATCACCATGCCGAAGAAAGAAGAGACCGCCGATACCACGTTCAGTACCTGACCGATGTAGCCGCTTACGCAGACAATACACAg > 2:338763/1‑100 (MQ=255) aaTGAGAGCAGCGAGGATCACCATGCCGAAGAAAGAAGAGACCGCCGATACCACGTTCAGTACCTGACCGATGTAGCCGCTTACGCAGACAATACACAgg > 1:576175/1‑100 (MQ=255) tGAGAGCAGCGAGGATCACCATGCCGAAGAAAGAAGAGACCGCCGATACCACGTTCAGTACCTGACCGATGTAGCCGCTTACGCAGACAATACACAGGGc > 2:2576328/1‑100 (MQ=255) tGAGAGCAGCGAGGATCACCATGCCGAAGAAAGAAGAGACCGCCGATACCACGTTCAGTACCTGACCGATGTAGCCGCTTACGCAGACAATACACAGGGc > 1:1666795/1‑100 (MQ=255) gagaGCAGCGAGGATCACCATGTCGAAGAAAGAAGAGACCGCCGATACCACGTTCAGTACCTGACCGATGTAGCCGCTTACGCAGACAATACACAGGGCg > 1:2516204/1‑100 (MQ=255) gagaGCAGCGAGGATCACCATGCCGAAGAAAGAAGAGACCGCCGATACCACGTTCAGTACCTTACCGATGTAGCCGCTTACGCAGACAATACACAGGGCg < 2:1619397/100‑1 (MQ=255) | AGCGAATCATCAAGCCACAAGCCGGGGCAAGAAACATAAATATCCCCGTCACTAGTTCATTACCGCCAGTTAACCACATGGTGAAGTAATAAATGAGTGCAGCGAGGATCACCATGCCGAAGAAAGAAGAGACCGCCGATACCACGTTCAGTACCTGACCGATGTAGCCGCTTACGCAGACAATACACAGGGCG > NC_000913/2495076‑2495269 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |