BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	2,530,925	T→C	100%	Q103Q (CAA→CAG)	zipA ←	FtsZ stabilizer

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,530,925	0	T	C	96.8%	466.9 / 0.7	144	Q103Q (CAA→CAG)	zipA	FtsZ stabilizer
Reads supporting (aligned to +/- strand): ref base T (2/4); new base C (66/72); total (68/76)
Fisher's exact test for biased strand distribution p-value = 6.84e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

Only 100 of 144 total aligned reads displayed.

TTCAGGCTGCGGCTGATAGGCAGGCTGCTGCACCGGCTGCGCTGGATGCGGAGCATGTTGCGGCGGTACCTGCGCTTCAGGCGGCTGCTGGACCGGTTGACGCGGCTGCGCAGACGCATAAGGCGGTTGGTACTGGTGTTGCGGCGACGGACGAGCAGCCTCATGCTCCTGAGCGTTAGCCGGGGCATGATTC  >  NC_000913/2530829‑2531021

 tCAGGCTGCGGCTGATAGGCAGGCTGCTGCACCGGCTGCGCTGGATACGGAGCATGTTGCGGCGGTACCTGCGCTTCAGGCGGCTGCTGGACCGGCTGAc                                                                                              >  1:35418/1‑100 (MQ=255)

   aGGCTGCGGCTGATAGGCAGGCTGTTGCACCGGCTGCGCTTGATGCTGAGCATGTTGCGGCGGTACCTGCGCTTCAGGCGGCTGCTGGACCGGTTGAcgc                                                                                            <  1:1046818/100‑1 (MQ=255)

   aGGCTGCGGCTGATAGGCAGGCTGCTGCACCGGCTGCGCTGGATGCGGAGCATGTTGCGGCGGTACCTGCGCTTCAGGCGGCTGCTGGACCGGCTGAagc                                                                                            <  1:3013617/100‑1 (MQ=255)

    ggCTGCGGCTGATAGGCAGGCTGCTGCACCGGCTGCGCTGGATGCGGAGCATGTTGCGGCGGTACCTGCGCTTCAGGCGGCTGCTGGACCGGCTGAcgcg                                                                                           >  1:490360/1‑100 (MQ=255)

    ggCTGCGGCTGATAGGCAGGCTGCTGCACCGGCTGCGCTGGATGCGGAGCATGTTGCGGCGGTACCTGCGCTTCAGGCGGCTGCTGGACCGGCTGAcgcg                                                                                           >  2:1574805/1‑100 (MQ=255)

    ggCTGCGGCTGATAGGCAGGCTGCTGCACCGGCTGCGCTGGATGCGGAGCATGTTGCGGCGGTACCTGCGCTGCAGGCCGCTGCTGGACCGGCTGAcgc                                                                                            >  2:2387768/1‑99 (MQ=255)

    ggCTGCGGCTGATAGGAAGGCTGCTGCACCGGCTGCGCTGGATGCGGAGCATGTTGCGGCGGTACCTGCGCTTCAGGCGGCTGCTGGACAGGCTGAcgcg                                                                                           >  1:1346823/1‑100 (MQ=255)

      cTGCGGCTGATAGGCAGGCTGCTGCACCGGCTGCGCTGGATGCGGAGCATGTTGCGGCGGTACCTGCGCTTCAGGCGGCTGCTGGACCGGCTGACGCGGc                                                                                         >  2:2078812/1‑100 (MQ=255)

         cGGCTGATAGGCAGGCTGCTGCACCGGCTGCGCTGGATGCGGAGCATGTTGCGGCGGTACCTGCGCTTCAGGCGGCTGCTGGACCGGCTGACACGGCTg                                                                                       <  2:690518/99‑1 (MQ=255)

          ggCTGATAGGCAGGCTGCTGCACCGGCGGCGCTGGATGCGGAGCATGTTGCGGCGGTACCTGCGCTTCAGGCGGCTGCTGGACCGGCTGACGCGGCTgcg                                                                                     <  2:826814/100‑1 (MQ=255)

              gATAGGCAGGCTGCTGCACCGGCTGCGCTGGATGCGGAGCATGTTGCGGCGGTACCTGCGCTTCAGGCGGCTGCTGGACCGGCTGACGCGGCTGCGCAGa                                                                                 >  1:2134392/1‑100 (MQ=255)

                tAGGCAGGCTGCTGCACCGGCTGCGCTGTATGCGGAGCATGTTGCGGCGGTACCTGCGCTTCAGGCGGCTGCTGGACCGGCTGACGCGGCTGCGCAGACg                                                                               <  1:403866/100‑1 (MQ=255)

                tAGGCAGGCTGCTGCACCGGCTGCGCTGGATGCGGAGCATGTTGCGGCGGTACCTGCGCTTCAGGCGGCTGCTGGACCGGCTGACGCGGCTGCGCAGACg                                                                               <  1:2854382/100‑1 (MQ=255)

                tAGGCAGGCTGCTGCACCGGCTGCGCTGGATGCGGAGCATGTTGCGGCGGTACCTGCGCTTCAGGCGGCTGCTGGACCGGCTGACGCGGCTGCGCAGACg                                                                               >  1:2453943/1‑100 (MQ=255)

                tAGGCAGGCTGCTGCACCGGCTGCGCTGGATGCGGAGCATGTTGCGGCGGTACCTGCGCTTCAGGCGGCTGCTGGACCGGCTGACGCGGCTGCGCAGACg                                                                               <  1:195098/100‑1 (MQ=255)

                 aggcaggcTGCTGCACCGGCTGCGCCGGATGCGGTGCATGTTGCGGCTGTATCTGCGCTTCAGCCGGCTGCTGGACCTGCTGTCGCGGCTGCGAAGACg                                                                               <  2:155175/99‑1 (MQ=255)

                  ggcaggcTGCTGCACCGGCTGCGCTGGATGCGGAGCATGTTGCTGCGGTACCTGCGCTTCAGGCGGCTGCTGGACCGGCTGACGCGACTGCGCAGACGCa                                                                             <  1:906098/100‑1 (MQ=255)

                  ggcaggcTGCTGCACCGGCTGCGCTGGATGCGGAGCATGTTGCGGCGGTACCTGCGCTTCAGGCGGCTGCTGGACCGGCTGACGCGGCTGCGCAGACGCa                                                                             >  2:2183183/1‑100 (MQ=255)

                  ggcaggcTGCTGCACCGGCTGCGCTGGATGCGGAGCATGTTGCGGCGGTACCTGCGCTTCAGGCGGCTGCTGGAACGGCTGACGCGGCTGCGCAGACGCa                                                                             >  1:2792802/1‑100 (MQ=255)

                    caggcTGCTGCACCGGCTGCGCTGGATGCGGAGCATGTTGCGGCGGTACCTTCGCTTCAGACGGCTACTGGACCGGCAGACGCGGCTGCGCAGACGCATa                                                                           >  1:2535781/1‑100 (MQ=255)

                    caggcTGCTGCACCGGCTGCGCTGGATGCGGAGCATGTTGCGGCGGTACCTGCGCTTCAGGCGGCTGCTGGACCGGCTGATGCGGCTGCGCAGACGCATa                                                                           <  2:719320/100‑1 (MQ=255)

                    caggcTGCTGCACCGGCTGCGCTGGATGCGGAGCATGTTGCGGCGGTACCTGCGCTTCAGGCGGCTGCTGGACCGGCTGACGCGGCTGCGCAGACGCATa                                                                           <  1:1442884/100‑1 (MQ=255)

                       gctgctGCACCGGCTGCGCTGGATGCGGAGCATGTTGCGGCGGTACCTGCGCTTCAGGCGGCTGCTGGACCGGCTGACGCGGCTGCGCAGACGCATAAgg                                                                        <  1:883327/100‑1 (MQ=255)

                       gctgctGCACCGGCTGCGCTGGATGCGGAGCATGTTGCGGCGGTACCTGCGCTTCAGGCGGCTGCTGGACCGGCTGACGCGGCTGCGCAGACGCATAAgg                                                                        <  1:609130/100‑1 (MQ=255)

                       gctgctGCACCGGCTGCGCTGGATGCGGAGCATGTTGCGGCGGTACCTGCGCTTCAGGCGGCTGCTGGACCGGCTGACGCGGCTGCGCAGACGCATAAgg                                                                        >  1:1707460/1‑100 (MQ=255)

                          gctgcACCGGCTGCGCTGGATGCGGAGCATGTTGCGGCGGTACCTGCGCTTCAGGCGGCTGCTGGACCGGCTGACGCGGCTGCGCAGACGCCTAAGGCgg                                                                     >  2:941846/1‑100 (MQ=255)

                          gctgcACCGGCTGCGCTGGATGCGGAGCATGTTGCGGCGGTACCTGCGCTTCAGGCGGCTGCTGGACCGGCTGACGCGGCTGCGCAGACGCATAAGGCgg                                                                     >  1:1290247/1‑100 (MQ=255)

                             gcACCGGCTGCGCTGGATGCGGAGCATGTTGCGGCGGTACCTGCGCTTCAGGCGGCTGCTGGACCGGCTGACGCGGCTGCGCAGACGCATAAGGCGGTTg                                                                  <  1:1998443/100‑1 (MQ=255)

                             gcACCGGCTGCGCTGGATGCGGAGCATGTTGCGGCGGTACCTGCGCTTCAGGCGGCTGCTGGACCGGCTGACGCGGCTGCGCAGACGCATAAGGCGGTTg                                                                  >  2:1862026/1‑100 (MQ=255)

                              cgccGGCTGCGCTGGATGCGGAGCATGTTGCGGCGGTACCTGCGCTTCAGGCGGCTGCTGGACCGGCTGACGCGGCTGCGCAGACGCATAAGGCGGTTgg                                                                 >  1:2054372/3‑100 (MQ=255)

                               aCCGGCTGCGCTGGATGCGGAGCATGTTGCGGCGGTACCTGCGCTTCAGGCGGCTGCTGGACCGGCTGACGCGGCTGCGCAGACGCATAAGGCGGTTGGt                                                                <  2:2604996/100‑1 (MQ=255)

                                ccGGCTGCGCTGGATGCGGAGCATGTTGCGGCGGTACCTGCGCTTCAGGCGGCTGCTGGACCGGCTGACACGGCTGCGCAGACGCATAAGGCGGTTGGTa                                                               >  1:128290/1‑100 (MQ=255)

                                 cGGCTGCGCTGGATGCGGAGCATGTTGCGGCGGTACCTGCGCTTCAGGCGGCTGCTGGACCGGCTGACGCGGCTGCGCAGACGCATAAGGCGGTTGGTAc                                                              <  1:960361/100‑1 (MQ=255)

                                    cTGCGCTGGATGCGGAGCATGTTGCGGCGGTACCTGCGCTTCAGGCGGCTGCTGGACCGGCTGACGCGGCTGCGCAGACGCATAAGGCGGTTGGTACTgg                                                           <  2:428096/100‑1 (MQ=255)

                                    cTGCGCTGGATGCGGAGCATGTTGCGGCGGTACCTGCGCTTCAGGCGGCTGCTGGACCGGCTGACGCGGCTGCGCAGACGCATAAGGCGGTTGGTACTgg                                                           <  1:1266609/100‑1 (MQ=255)

                                     tGCGCTGGATGCGGAGCATGTTGCGGCGGTACCTGCGCTTCAGGCGGCTGCTGGACCGGCTGACGCGGCTGCGCAGACGCATAAGGCGGTTGGTACTGgt                                                          <  2:1435808/100‑1 (MQ=255)

                                     tGCGCTGGATGCGGAGCATGTTGCGGCGGTACCTGCGCTTCAGGCGGCTGCTGGACCGGCTGACGCGGCTGCGCAGACGCATAAGGCGGTTGGTACTGgt                                                          <  1:654137/100‑1 (MQ=255)

                                     tGCGCTGGATGCGGAGCATGTTGCGGCGGTACCTGCGCTTCAGGCGGCTGCTGGACCGGCTGACGCGGCTGCGCAGACGCATAAGGCGGTTGGTACTGgt                                                          <  1:1745137/100‑1 (MQ=255)

                                       cgcTGGATGCGGAGCATGTTGCGGCGGTACCTGCGCTTCAGGCGGCTGCTGGACCGGCTGACGCGGCTGCGCAGACGCATAAGGCGGTTGGTACTGgtgt                                                        >  2:2877663/1‑100 (MQ=255)

                                        gcTGGATGCGGAGCATGTTGCGGCGGTACCTGCGCTTCAGGCGGCTGCTGGACCGGCTGACGCGGCTGCGCAGACGCATAAGGCGGTTGGTACTGGTGtt                                                       >  1:2385930/1‑100 (MQ=255)

                                        gcTGGATGCGGAGCATGTTGCGGCGGTACCTGCGCTTCAGGCGGCTGCTGGACCGGCTGACGCGGCTGCGCAGACGCATAAGGCGGTTGGTACTGGTGtt                                                       >  1:1372070/1‑100 (MQ=255)

                                         cTGGATGCGGAGCATGTTGCGGCGGTACCTGCGCTTCAGGCGGCTGCTGGACCGGCTGACGCGGCTGCGCAGACGCATAAGGCGGTTGGTACTGGTGTTg                                                      >  2:2314047/1‑100 (MQ=255)

                                         cTGGATGCGGAGCATGTTGCGGCGGTACCTGCGCTTCAGGCGGCTGCTGGACCGGCTGACGCGGCTGCGCAGACGCATAAGGCGGTTGGTACTGGTGTTg                                                      >  2:1047782/1‑100 (MQ=255)

                                          tGGATGCTGAGCATGTTGCGGCGGTACCTGCGCTTCAGGCGGCTGCTGGACCGGCTGACGCGGCTGCGCAGACGCATAAGGCGGTTGGTACTGGTGTTg                                                      <  1:552783/99‑1 (MQ=255)

                                            gATGCGGAGCATGTTGCGGCGGTACCTGCGCTTCAGGCGGCTGCTGGACCGGCTGACGCGGCTGCGCAGACGCATAAGGCGGTTGGTACTGGTGTTgcgg                                                   <  1:2579268/100‑1 (MQ=255)

                                             aTGCGGAGCATGTTGCGGCGGTACCTGCGCTTCAGGCGGCTGCTGGACCGGCTGACGCGGCTGCGCAGACGCATAAGGCGGTTGGTACTGGTGTTgcggc                                                  <  2:2200613/100‑1 (MQ=255)

                                             aTGCGGAGCATGTTGCGGCGGTACCTGCGCTTCAGGCGGCTGCTGGACCGGCTGACGCGGCTGCGCAGACGCATAAGGCGGTTGGTACTGGTGTTgcggc                                                  >  1:2206629/1‑100 (MQ=255)

                                             aTGCGGAGCATGTTGCGGCGGTACCTGCGCTTCAGGCGGCTGCTGGACCGGCTGACGCGGCTGCGCAGACGCATAAGGCGGTTGGTACTGGTGTTgcggc                                                  <  2:1397558/100‑1 (MQ=255)

                                             aTGCGGAGCATGTTGCGGCGGTACCTGCGCTTCAGGCGGCTGCTGGACCGGCTGACGCGGCTGCGCAGACGCATAAGGCGGTTGGTACTGGTGTTgcggc                                                  <  2:791499/100‑1 (MQ=255)

                                              tGCGGAGCATGTTGCGGCGGTACCTGCGCTTCAGGCGGCTGCTGGACCGGCTGACGCGGCTGCGCAGACGCATAAGGCGGTTGGTACTGGTGTTgcggcg                                                 <  1:2714741/100‑1 (MQ=255)

                                               gCGGAGCATGTTGCGGCGGTACCTGCGCTTCAGGCGGCTGCTGGACCGGCTGACGCGGCTGCGCAGACGCATAAGGCGGTTGGTACTGGTGTTGCGGCga                                                <  1:2370304/100‑1 (MQ=255)

                                                ccgAGCACGTTGCTGCGTGACCGGCGCTTCAGGCGGCTGCTGGACCGGTTGACGCGGCTGCGCAGAAGCCTACGGGGGTTGGTAATGGTGTTgaggc                                                  >  2:2845759/3‑97 (MQ=255)

                                                cGGAGCATGTTGCGGCGGTACCTGCGCTTCAGGCGGCTGCTGGACCGGCTGACGCGGCTGCGCAGACGCATAAGGCGGTTGGTACTGGTGTTGCGGCgac                                               >  2:10538/1‑100 (MQ=255)

                                                 ggAGCATGTTGCGGCGGTACCTGCGCTTCAGGCGGCTGCTGGACCGGCTGACGCGGCTGCGCAGACGCATAAGGCGGTTGGTACTGGTGTTGCGGCgacg                                              <  2:1777496/100‑1 (MQ=255)

                                                 ggAGCATGTTGCGGCGGTACCTGCGCTTCAGGCGGCTGCTGGACCGGCTGACGCGGCTGCGCAGAAGCATAAGGCGGTTGGTACTGGTGTTGCGGCgacg                                              >  1:2809343/1‑100 (MQ=255)

                                                   aGCATGTGGCGGCGGTACCTGCGCTTCAGGCGGCTGCTGGACCGGCTGACGCGGCTGCGCAGACGCATAAGGCGGTTGGTACTGGTGTTGCGGCgacgga                                            <  2:503649/100‑1 (MQ=255)

                                                    gCATGTTGCGGCGGTACCTGCGCTTCAGGCGGCTGCTGGACCGGCTGACGCGGCTGCGCAGACGCATAAGGCGGTTGGTACTGGTGTTGCGGCgacggac                                           <  2:2478934/100‑1 (MQ=255)

                                                    gCATGTTGCGGCGGTACCTGCGCTTCAGGCGGCTGCTGGACCGGCTGACGCGGCTGCGCAGACGCATAAGGCGGTTGGTACTGGTGTTGCGGCgacggac                                           >  2:2612280/1‑100 (MQ=255)

                                                     cATGTTGCGTCTGTACCTGTTTTTCAAGCGGCTGCTGGACCGGTTGACACGGCTGCGCAGACGCATAAGGCGGTTGGTACTGGTGTTGCGGCgacgg                                             <  1:3022993/97‑1 (MQ=255)

                                                     cATGTTGCGGCGGTACCTGCGCTTCAGGCGGCTGCTGGACCGGCTGACGCGGCTGCGCAGACGCATAAGGCGGTTGGTACTGGTGTTGCGGCgacggacg                                          <  2:2444380/100‑1 (MQ=255)

                                                     cATGTTGCGGCGGTACCTGCGCTTCAGGCGGCTGCTGGACCGGCTGACGCGGCTGCGCAGACGCATAAGGCGGTTGGTACTGGTGTTGCGGCgacggacg                                          <  1:2117047/100‑1 (MQ=255)

                                                     cATGTTGCGGCGGTACCTGCGCTTCAGGCGGCTGCTGGACCGGCTGACGCGGCTGCGCAGACGCATAAGGCGGTTGGTACTGGTGTTGCGGCgacggacg                                          <  2:1372527/100‑1 (MQ=255)

                                                       tGTTGCGGCGGTACATGCGCTACAAGCGGCTGCTGGACCCGCTGACGCGGCTGCGCAGACGCATAAGTCGGTTGGTACTGGTGGTGCGGCTACGGACTag                                        >  2:2536630/1‑100 (MQ=255)

                                                         ttGCGGCGGTACCTGCGCTTCAGGCGGCTGCTGGACCGGCTGACGCGGCTGCGCAGACGCATAAGGCGGTTGGTACTGGTGTTGCGGCGACGGACGagca                                      <  2:1325704/100‑1 (MQ=255)

                                                          tGCGGCGGTACCTGCGCTTCAGGCGGCTGCTGGACCGGCTGACGCGGCTGCGCAGACGCATAAGGCGGTTGGTACTGATGTTGCGGCGACGGACGagcag                                     >  2:2270056/1‑100 (MQ=255)

                                                            cggcggTACCTGCGCTTCAGGCGGCTGCTGGACCGGCTGACGCGGCTGCGCAGACGCATAAGGCGGTTGGTACTGGTGTTGCGGCGACGGACGAGCAGcc                                   >  1:833833/1‑100 (MQ=255)

                                                            cggcggTACCTGCGCTTCAGGCGGCTGCTGGACCGGCTGACGCGGCTGCGCAGACGCATAAGGCGGTTGGTACTGGTGTTGCGGCGACGGACGAGCAGcc                                   <  2:1972755/100‑1 (MQ=255)

                                                              gcggTACCTGCGCTTCAGGCGGCTGCTGGACCGGCTGACGCGGCTGCGCAGACGCATAAGGCGGTTGGTACTGGTGTTGCGGCGACGGACGAGCAGCCTC                                 >  1:1499373‑M1/1‑100 (MQ=255)

                                                               cggTACCTGCGCTTCAGGCGGCTGCTGGACCGGCTGACGCGGCTGCGCAGACGCATAAGGCGGTTGGTACTGGTGTTGCGGCGACGGACGAGCAGCCTCA                                <  1:2937458‑M1/100‑1 (MQ=255)

                                                                ggTACCTGCGCTTCAGGCGGCTGCTGGACCGGCTGACGCGGCTGCGCAGACGCATAAGGCGGTTGGTACTGGTGTTGCGGCGACGGACGAGCAGCCTCAT                               >  1:177596‑M1/1‑100 (MQ=255)

                                                                ggTACCTGCGCTTCAGGCGGCTGCTGGACCGGCTGACGCGGCTGCGCAGACGCATAAGGCGGTTGGTACTGGTGTTGCGGCGACGGACGAGCAGCCTCAT                               >  1:2283108‑M1/1‑100 (MQ=255)

                                                                  tACCTGCGCTTCAGGCGGCTGCTGGACCGGCGGACGCGGCTGCGCAGACGCATAAGGCGGTTGGTACTGGTGTTGCGGCGACGGACGAGCAGCCTCATGC                             <  2:2280765‑M1/100‑1 (MQ=255)

                                                                    ccTGCGCTTCAGGCGGCTGCTGGACCGGCTGACGCGGCTGCGCAGACGCATACGGCGGTTGGTACTGGTGTTGCGGCGCCGGACGAGCAGCCTCATGCTc                           >  2:1689476/1‑100 (MQ=255)

                                                                    ccTGCGCTTCAGGCGGCTGCTGGACCGGCTGACGCGGCTGCGCAGACGCATAAGGCGGTTGGTACTGGTGTTGCGGCGACGGACGAGCAGCCTCATGC                             >  1:1825545‑M1/1‑98 (MQ=255)

                                                                     cTGCGCTTCAGGCGGCTGCTGGACCAGCTGACGCGGCTGCGCAGACGCATAAGGCGGTTGGTACTGGTGTTGCGGCGACGGACGAGCAGCCTCATGCTcc                          >  2:570823/1‑100 (MQ=255)

                                                                     cTGCGCTTCACGCGGCTGCTGGACCGGCTGACGCGGCTGCGCAGACGCATAAGGCGGTTGGTACTGGTGTTGCGGCGACGGACGAGCAGCCTCATGCTcc                          >  2:2136655/1‑100 (MQ=255)

                                                                      tGCGCTTCAGGCGGCTGCTGGACCGGTTGACGCGGCTGCGCAGACGCATAAGGCGGTTGGTACTGGTGTTGCGGCGACGGACGAGCAGCCTCATGCTCCt                         <  2:923560/100‑1 (MQ=255)

                                                                        cgcTTCAGGCGGCTGCTGGACCGGCTGACGCGGCTGCGCAGACGCATAAGGCGGTTGGTACTGGTGTTGCGGCGACGGACGAGCAGCCTCATGCTCCTGa                       <  2:1135992/100‑1 (MQ=255)

                                                                         gcTTCAGGCGGCTGCTGGACCGGCTGACGCGGCTGCGCAGACGCATAAGGCGGTTGGTACTGGTGTTGCGGCGACGGACGAGCAGCCTCATGCTCCTGAg                      <  1:2183206/100‑1 (MQ=255)

                                                                           ttCAGGCGGCTGCTGGACCGGCTGACGCGGCTGCGCAGACGCATAAGGCGGTTGGTACTGGTGTTGCGGCGACGGACGAGCAGCCTCATGCTCCTGAGCg                    <  1:2826724/100‑1 (MQ=255)

                                                                              aGGCGGCTGCTGGACCGGCTGACGCGGCTGCGCAGACGCATAAGGCGGTTGGTACTGGTGTTGCGGCGACGGACGAGCAGCCTCATGCTCCTGAGCGTTa                 <  2:1773241/100‑1 (MQ=255)

                                                                                 cggcTGCTGGACCGGCTGACGCGGCTGCGCAGACGCATAAGGCGGTTGGTACTGGTGTTGCGGCGACGGACGAGCAGCCTCATGCTCCTGAGAGTTAGcc              >  1:1325070/1‑100 (MQ=255)

                                                                                   gctgctGGACCGGCTGACGCGGCTGCGCAGACGCATAAGGCGGTTGGTACTGGTGTTGCGGCGACGGACGAGCAGCCTCATGCTCCTGAGCGTTAGCCgg            >  2:1298924/1‑100 (MQ=255)

                                                                                    ctgctgGACCGGCTGACGCGGCTGCGCAGACGCATAAGGCGGTTGGTACTGGTGTTGCGGCGACGGACGAGCAGCCTCATGCTCCTGAGCGTTAGCCggg           <  1:2515604/100‑1 (MQ=255)

                                                                                    ctgctgGACCGGCTGACGCGGCTGCGCAGACGCATAAGGCGGTTGGTACTGGTGTTGCGGCGACGGACGAGCAGCCTCATGCTCCTGAGCGTTAGCCggg           >  2:1795226/1‑100 (MQ=255)

                                                                                     tgctgGACCGGCTGACGCGGCTGCGCAGACGCATAAGGCGGTTGGTACTGGTGTTGCGGCGACGGACGAGCAGCCTCATGCTCCTGAGCGTTAGCCGGGg          <  1:91978‑M2/100‑2 (MQ=255)

                                                                                      gctgGACCGGCTGACGCGGCTGCGCAGACGCATAAGGCGGTTGGTACTGGTGTTGCGGCGACGGACGAGCAGCCTCATGCTCCTGAGCGTTAGCCGGGgc         >  1:2299176‑M2/1‑98 (MQ=255)

                                                                                      gctgGACCGGCTGACGCGGCTGCGCAGACGCATAAGGCGGTTGGTACTGGTGTTGCGGCGACGGACGAGCAGCCTCATGCTCCTGAGCGTTAGCCGGGgc         <  2:1234813‑M2/100‑3 (MQ=255)

                                                                                       ctgGACCGGCTGACGCGGCTGCGCAGACTCATAAGGCGGTTGGTACTGGTGTTGCGGCGACGGACGAGCAGCCTCATGCTCCTGAGCGTTAGCCGGGgca        >  2:465411‑M2/1‑97 (MQ=255)

                                                                                        tgGACCGGCTGACGCGGCTGCGCAGACGCATAAGGCGGTTGGTACTGGTGTTGCGGCGACGGACGAGCAGCCTCATGCTCCTGAGCGTTAGCCGGGgcat       <  1:1004566‑M2/100‑5 (MQ=255)

                                                                                         ggACCGGCTGACGCGGCTGCGCAGACGCATAAGGCGGTTGGTACTGGTGTTGCGGCGACGGACGAGCAGCCTCATGCcccggctaacgctcaggagcatg      <  1:545108‑M1/100‑24 (MQ=255)

                                                                                         ggACCGGCTGACGCGGCTGCGCAGACGCATAAGGCGGTTGGTACTGGTGTTGCGGCGACGGACGAGCAGCCTCATGCcccggctaacgctcaggagcatg      <  1:1301701‑M1/100‑24 (MQ=255)

                                                                                         ggACCGGCTGACGCGGCTGCGCAGACGCATAAGGCGGTTGGTACTGGTGTTGCGGCGACGGACGAGCAGCCTCATGCTCCTGAGCGTTAGCCGGGgcatg      <  1:514388‑M2/100‑6 (MQ=255)

                                                                                         ggACCGGCTGACGCGGCTGCGCAGACGCATAAGGCGGTTGGTACTGGTGTTGCGGCGACGGACGAGCAGCCTCATGCTCCTGAGCGTTAGCCGGGgcatg      <  2:169987‑M2/100‑6 (MQ=255)

                                                                                          gcccggCTGACGCGGCTGCGAAGACGCATAAGGCGGTTGGTACTGGTGTTGCGGCGACGGACGAGCAGCCTCATGCcccggctaacgctcaggagcagn      <  2:328603‑M1/97‑24 (MQ=255)

                                                                                          gACCGGCTGACGCGGCTGCGCAGACGCATAAGGCGGTTGGTACTGGTGTTGCGGCGACGGACGAGCAGCCTCATGCcccggctaacgctcaggagcatga     <  2:2651778‑M1/100‑25 (MQ=255)

                                                                                           aCCGGCTGACGCGGCTGCGCAGACGCATAAGGCGGTTGGTACTGGTGTTGCGGCGACGGACGAGCAGCCTCATGCcccggctaacgctcaggagcatgag    <  1:2910879‑M1/100‑26 (MQ=255)

                                                                                             cGGCTGACGCGGCTGCGCAGACGCATAAGGCGGTTGGTACTGGTGTTGCGGCGACGGACGAGCAGCCTCATGCcccggctaacgctcaggagcatgaggc  <  2:2969467‑M1/100‑28 (MQ=255)

                                                                                             cGGCTGACGCGGCTGCGCAGACGCATAAGGCGGTTGGTACTGGTGTTGCGGCGACGGACGAGCAGCCTCATGCcccggctaacgctcaggagcatgaggc  <  1:1086563‑M1/100‑28 (MQ=255)

                                                                                             cGGCTGACGCGGCTGCGCAGACGCATAAGGCGGTTGGTACTGGTGTTGCGGCGACGGACGAGCAGCCTCATGCTCCTGAGCGTTAGCCGGGGCATGATTc  >  2:397952/1‑100 (MQ=255)

TTCAGGCTGCGGCTGATAGGCAGGCTGCTGCACCGGCTGCGCTGGATGCGGAGCATGTTGCGGCGGTACCTGCGCTTCAGGCGGCTGCTGGACCGGTTGACGCGGCTGCGCAGACGCATAAGGCGGTTGGTACTGGTGTTGCGGCGACGGACGAGCAGCCTCATGCTCCTGAGCGTTAGCCGGGGCATGATTC  >  NC_000913/2530829‑2531021

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`