Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,534,206 T→G 100% R47R (CGT→CGG ptsI → PEP‑protein phosphotransferase of PTS system (enzyme I)

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,534,2060TG98.4% 317.9 / ‑4.3 97R47R (CGT→CGGptsIPEP‑protein phosphotransferase of PTS system (enzyme I)
Reads supporting (aligned to +/- strand):  ref base T (0/2);  new base G (60/34);  total (61/36)
Fisher's exact test for biased strand distribution p-value = 1.38e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.65e-01

AAGCTCTGCTTCTGAAAGAAGACGAAATTGTCATTGACCGGAAAAAAATTTCTGCCGACCAGGTTGATCAGGAAGTTGAACGTTTTCTGAGCGGTCGTGCCAAGGCATCAGCCCAGCTGGAAACGATCAAAACGAAAGCTGGTGAAACGTTCGGTGAAGAAAAAGAAGCCATCTTTGAAGGGCATATTATGCT  >  NC_000913/2534109‑2534301
                                                                                                 |                                                                                               
aaGCTCTGCTTCTGAAAGAAGACGAAATTGTCATTGACCTGAAAAAAATTTCTGCCGACCAGGTTGATCAGGAAGTTGAACGTTTTCTGAGCGGTCGGGc                                                                                               <  1:785835/100‑1 (MQ=255)
  gCTCTGCTTCTGAAAGAAGACGAAATTGTCATTGACCGGAAAAAAATTTCTGCCGACCAGGTTGATCAGGAAGTTGAACGTTTTCTGAGCGGTCGGGcc                                                                                              >  2:803433/1‑99 (MQ=255)
  gCTCTGCTTCTGAAAGAAGACGAAATTGTCATTGACCGGAAAAAAATTTCTGCCGACCAGGTTGATCAGGAAGTTGAACGTTTTCTGAGCGGTCGGGCCa                                                                                             <  1:1453564/100‑1 (MQ=255)
  gCTCTGCTTCTGAAAGAAGACGAAATTGTCATTGACCGGAAAAAAATTTCTGCCGACCAGGTTGATCAGGAAGTTGAACGTTTTCTGAGCGGTCGGGCCa                                                                                             >  2:1448264/1‑100 (MQ=255)
   ctctGCTTCTGAAAGAAGACGAAATTGTCATTGACCGGAAAAAAATTTCTGCCGACCAGGTTGATCAGGAAGTTGAACGTTTTCTGAGCGGTCGGGCCaa                                                                                            >  2:1898957/1‑100 (MQ=255)
     ctGCTTCTGAAAGAAGACGAAATTGTCATTGACCGGAAAAAAATTTCTGCCGACCAGGTTGATCAGGAAGTTGAACGTTTTCTGAGCGGTCGGGCCAAgg                                                                                          >  2:936657/1‑100 (MQ=255)
      tGCTTCTGAAAGAAGACGAAATTGTCATTGACCGGAAAAAAATTTCTGCCGACCAGGTTGATCAGGAAGTTGAACGTTTTCTGAGCGGTCGGGCCAAgg                                                                                          <  2:645017/99‑1 (MQ=255)
        cTTCTGAAAGAAGACGAAATTGTCATTGACCGGAAAAAAATTTCTGCCGACCAGGTTGATCAGGAAGTTGAACGTTTTCTGAGCGGTCGGGCCAAGGCAt                                                                                       >  1:1947317/1‑100 (MQ=255)
        cTTCTGAAAGAAGACGAAATTGTCATTGACCGGAAAAAAATTTCTGCCGACCAGGTTGATCAGGAAGTTGAACGTTTTCTGAGCGGTCGGGCCAAGGCAt                                                                                       >  2:1530147/1‑100 (MQ=255)
          tCTGAAAGAAGACGAAATTGTCATTGACCGGAAAAAAATTTCTGCCGACCAGGTTGATCAGGAAGTTGAACGTTTTCTGAGCGGTCGGGCCAAGGCATCa                                                                                     <  1:256105/100‑1 (MQ=255)
          tCTGAAAGAAGACGAAATTGTCATTGACCGGAAAAAAATTTCTGCCGAACAGGTTGATCAGGAAGTTGAACGATTTCTGAGCGGTCGGGCCAAGGCATCa                                                                                     >  1:2739769/1‑100 (MQ=255)
           cTGAAAGAAGACGAAATTGTCATTGACCGGAAAAAAATTTCTGCCGACCAGGTTGATCAGGACGTTGAACGTTTTCTGAGCGGTCGGGCCAAGGCATCAg                                                                                    >  2:446898/1‑100 (MQ=255)
           cTGAAAGAAGACGAAATTGTCATTGACCGGAAAAAAATTTCTGCCGACCAGGTTGATCAGGAAGTTGAACGTTTTATGAGCGGTCGGGCCAAGGCATCAg                                                                                    >  1:2581467/1‑100 (MQ=255)
            ttaaaGAAGACTAAATTGTCATTTACCGGAAAAAAATTTCTGCCGACCAGGTTGATCAGGAAGTTGAACGTTTTCTGAGCGGTCGGGCCAAGGCATc                                                                                      <  2:1908400/95‑1 (MQ=255)
            tGAAAGAAGACGAAATTGTCATTGACCGGAAAAAAATTTCTGCCGACCAGGTTGATCAGGAAGTTGAACGTTTTCTGAGCGGTCGGGCCAAGGCATCAGc                                                                                   >  1:883480/1‑100 (MQ=255)
              aatgaagaCGACATCGCTATTGACAGCAAATAAAGTCTTGCCGACCAGGTTGATCATACAGTTGATCTTTTCCTTAGCGGGTGTGTCAAGGAATCAGc                                                                                   <  2:2595046/95‑1 (MQ=255)
               aataagaCGAAATTGTTATTGATCGGAAAAAAATTTCTGCCGACCAGGTTGATCAGGAAGTTGAACGTTTTCTGAGCGGTCGGGCCAAGGCATCAGCCCa                                                                                <  1:394073/97‑1 (MQ=255)
               aagaagACGAAATTGTCATTGACCGGAAAAAAATTTCTGCCGACCAGGTTGATCAGGAAGTTGAACGTTTTCTGAGCGGTCGGGCCAAGGCATCAGCCCa                                                                                <  2:1698989/100‑1 (MQ=255)
                   agaCGGAATTGTCATTGACCGGAAAAGAATTTGTGCCGACCAGGTTGATCAGGGAGGTGAACGTTTTCTGAGCGGTCGTGGCAAGGCATCAGCCCAGCTg                                                                            <  1:1773909/100‑1 (MQ=255)
                   agaCGAAATTGTCATTGACCGGAAAAAAATTTCTGCCGACCAGGTTGATCAGGAAGTTGAACGTTTTCTGAGCGGTCGGGCCAAGGCATCAGCCCAGCTg                                                                            >  1:2421574/1‑100 (MQ=255)
                      cGAAATTGTCATTGACCGGAAAAAAATTTCTGCCGACCAGGTTGATCAGGAAGTTGAACGTTTTCTGAGCGGTCGGGCCAAGGCATCAGCCCAGCTGGaa                                                                         >  1:2618819/1‑100 (MQ=255)
                       gAAATTGTCATTGACCGGAAAAAATTTCTGCCGACCAGGTTGATCAGGAAGTTGAACGTTTTCTGAGCGGTCGGGCCAAGGCATCAGCCCAGCTGGAAAc                                                                       >  2:367408/1‑100 (MQ=255)
                        aaaTTGTCATTGACCGGAAAAAAATTTCTGCCGTCCAGGTTGATTAGGAAGTTGAACGTTTTCTGAGCGGTCGGGCCAAGGCATCAGCCCAGCTGGAAAc                                                                       <  1:837544/100‑1 (MQ=255)
                        aaaTTGTCATTGACCGGAAAAAAATTTCTGCCGACCAGGTTGATCAGGAAGTTGAACGTTTTCTGAGCGGTCGGGCCAAGGCATCAGCCCAGCTGGAAAc                                                                       <  1:878146/100‑1 (MQ=255)
                         aaTTGTCATTGACCGGAAAAAAATTTCTGCCGACCAGGTTGATCATGAAGTTGAACGTTTTCTTAGCGGTCGGGCCAAGGCATCAGCCCAGCTGGAAAc                                                                       <  2:917039/99‑1 (MQ=255)
                         aaTTGTCATTGACCGAAAAAAAATTTCTGACGACCAGGTTGATAAGGAAGTTGAACGATTTCTGAGCGATCGGGCCAAGGCATCAGCCCAGCTGGaaa                                                                        >  2:308678/1‑98 (MQ=255)
                            tGTCATTGACCGGAAAAAAATTTCTGCCGACCAGGTTGATCAGGAAGTTGAACGTTTTCTGAGCGGTCGGGCCAAGGCATCAGCCCAGCTGGAAACGATc                                                                   <  1:2126799/100‑1 (MQ=255)
                            tGTCATTGACCGGAAAAAAATTTCTGCCGACCAGGTTGATCAGGAAGTTGAACGTTTTCTGAGCGGTCGGGCCAAGGCATCAGCCCAGCTGGAAACGATc                                                                   <  2:858521/100‑1 (MQ=255)
                             gatcttGACCGGAAAAAAATTTCTGCCGACCAGGTTGATCAGGAAGTTGAACGTTTTCTGAGCGGTCGGGCCAAGGCATCAGCCCAGCTGGAAACGATCa                                                                  <  2:666213/96‑1 (MQ=255)
                             gTCATTGACCTGAAAAAAATTTCTCCCGACCAAGTTGATCAGGAAGTTGAACGTTTTCTGAGCGGTCGGGCCAAGGCATCAGCCCAGCTGGAAACGATCa                                                                  <  2:1340356/100‑1 (MQ=255)
                             gTCATTGACCGGAAAAAAATTTCTGCCGACCAGGTTGATCAGGAAGTTGAACGTTTTCTGAACGGTCGGGCCAAGGCATCAGCCCAGCTGGAAACGATCa                                                                  >  1:1137945/1‑100 (MQ=255)
                               cATTGACCGGAAAAAAATTTCTGCCGACCAGGTTGATCAGGAAGTTGAACGTTTTCTGAGCGGTCGGGCCAAGGCATCAGCCCCGCTGGAAACGATCaaa                                                                >  2:529935/1‑100 (MQ=255)
                                aTTGACCGGAAAAAAATTTCTGCCGACCAGGTTGATCAGGAAGTTGAACGTTTTCTGAGCGGTCGGGCCAAGGCATCAGCCCAGCTGGAAACGATCaaaa                                                               <  1:956501/100‑1 (MQ=255)
                                  tGACCGGAAAAAAATTTCTGCCGACCAGGTTGATCAGGAAGTTGAACGTTTTCTGAGCGGTCGGGCAAAGGCATCAGCCCAGCTGGAAACGATCaagatc                                                             >  1:666217/1‑96 (MQ=255)
                                   gACCGGAAAAAAATTTCTGCCGACCAGGTTGATCAGGAAGTTGAACGTTTTCTGAGCGGTCGGGCCAAGGCATCAGCCCAGCTGGAAACGATCAAAACGa                                                            >  1:1116405/1‑100 (MQ=255)
                                   gACCGGAAAAAAATTTCTGCCGACCAGGTTGATCAGGAAGTTGAACGTTTTCTGAGCGGTCGGGCCAAGGCATCAGCCCAGCTGGAAACGATCAAAACGa                                                            >  1:1760783/1‑100 (MQ=255)
                                    aCCGGAAAAAAATTTCTGCCGACCAGGTTGATCAGGAAGTTGAACGTTTTCTGAGCGGTCGGGCCAAGGCATCAGCCCAGCTGGAAACGATCAAAACGaa                                                           >  1:2010053/1‑100 (MQ=255)
                                      cttccgatcttttCTGCCGACCAGGTTGATCAGGAAGTTGAACGTTTTCTGAGCGGTCGGGCCAAGGCATCAGCCCAGCTGGAAACGATCAAAACGAAAg                                                         <  2:650554/90‑1 (MQ=255)
                                       ggAAAAAAATTTCTGCCGACCAGGTTGATCAGGAAGTTGAACGTTTTCTGAGCGGTCGGGCCAAGGCATCAGCCCAGCTGGAAACGATCAAAACGAAAGc                                                        >  1:2515200/1‑100 (MQ=255)
                                        gAAAAAAATTTCTGCCGACCAGGTTGATCAGGAATTTGAACGTTTTCTGATCAGTCAGGCCAAAACATCAGCCCAGCTGGAAACGATCAAAACGAAAGCt                                                       >  1:1807649/1‑100 (MQ=255)
                                        gAAAAAAATTTCTGCCGACCAGGTTGATCAGGAAGTTGAACGTTTTCTGAGCGGTCGGGCCAAGGCATCAGCCCAGCTGGAAACGATCAAAACGAAAGCt                                                       >  2:2764354/1‑100 (MQ=255)
                                          aaaaaaTTTCTGCCGACCAGGTTGATCAGGAAGTTGAACGTTTTCTGAGCGGTCGGGCCAAGGCATCAGCCCAGCTGGAAACGATCAAAACGAAAGCTgg                                                     >  2:2105315/1‑100 (MQ=255)
                                          aaaaaaTTTCTGCCGACCAGGTTGATCAGGAAGTTGAACGTTTTCTGAGCGGTCGGGCCAAGGCATCAGCCCAGCTGGAAACGATCAAAACGAAAGCTgg                                                     >  1:1883283/1‑100 (MQ=255)
                                          aaaaaaTTTCTGCCGACCAGGTTGATCAGGAAGTTGAACGTTTTCTGAGCAGTCGGGCCAAGGCATCAGCCCAGCTGGAAACGATCAAAACGAAAGCTgg                                                     >  1:765217/1‑100 (MQ=255)
                                           aaaaaTTTCTGCCGACCAGGTTGATCAGGAAGTTGAACGTTTTCTGAGCGGTCGGGCCAAGGCATCAGCCCATCTGGAAACGATCAAAACGAAAGCTGGt                                                    <  2:1505460/100‑1 (MQ=255)
                                            aaaaTTTCTGCCGACCAGGTTGATCAGGAAGTTGAACGTTTTCTGAGCGGTCGGGCCAAGGCATCAGCCCAGCTGGAAACGATCAAAACGAAAGCTGGTg                                                   <  1:1513254/100‑1 (MQ=255)
                                            aaaaTTTCTGCCGACCAGGTTGATCAGGAAGTTGAACGTTTTCTGAGCGGTAGGGCCAAAACAACAGCCCAGCTGGAAACGATCAAAACGAAAGCTGGTg                                                   >  1:332238/1‑100 (MQ=255)
                                                tttCTGCCGACCAGGTTGATCAGGAAGTTGAACGTTTTCTGAGCGGTCGGGCCAAGGCATCAGCCCAGCTGGAAACGATCAAAACGAAAgagatcggaag                                               >  1:650558/1‑90 (MQ=255)
                                                tttCTGCCGACCAGGTTGATCAGGAAGTTGAACGTTTTCTGAGCGGTCGGGCCAAGGCATCAGCCCAGCTGGAAACGATCAAAACGAAAGCTGGTGAAAc                                               >  1:2651464/1‑100 (MQ=255)
                                                 ttCTGCCGACCAGGTTGATCAGGAAGTTGAACGTTTTCTGAGCGGTCGGGCCAAGGCATCAGCCCAGCTGGAAACGATCAAAACGAAAGCTGGTGAAACg                                              >  2:2475134/1‑100 (MQ=255)
                                                   cTGCCGACCAGGTTGATCAGGAAGTTGAACGTTTTCTGAGCGGTCGGGCCAAGGCATCAGCCCAGCTGGAAACGATCAAAACGAAAGCTGGTGAAACGtt                                            >  1:219327/1‑100 (MQ=255)
                                                    tGCCGACCAGGTTGATCAGGAAGTTGAACGTTTTCTGAGCGGTCGGGCCAAGGCATCAGCCCAGCTGGAAACGATCAAAACGAAAGCTGGTGAAACGTTc                                           <  2:2618787/100‑1 (MQ=255)
                                                     gCCGACCAGGTTGATCAGGAAGTTGAACGTTTTCTGAGCGGTCGGGCCAAGGCATCAGCCCAGCTGGAAACGATCAAAACGAAAGCTGGTGAAACGTTCg                                          >  2:1083390/1‑100 (MQ=255)
                                                     gCCGACCAGGTTGATCAGGAAGTTGAACGTTTTCTGAGCGGTCGGGCCAAGGCATCAGCCCAGCTGGAAACGATCAAAACGAAAGCTGGTGAAACGTTCg                                          >  2:1408708/1‑100 (MQ=255)
                                                      ccGACCAGGTTGATCAGGAAGTTGAACGTTTTCTGAGCGGTCGGGCCAAGGCATCAGCCCAGCTGGAAACGATCAAAACGAAAGCTGGTGAAACGTTCgg                                         >  2:2912182/1‑100 (MQ=255)
                                                      ccGACCAGGTTGATCAGGAAGTTGAACGTTTTCTGAGCGGTCGGGCCAAGGCATCAGCCCAGCTGGAAACGATCAAAACGAAAGCTGGTGAAACGTTCgg                                         >  1:2413307/1‑100 (MQ=255)
                                                       cgcccAGGTTGATCAGGAAGTTGAACGTTTTCTGAGCGGTCGGGCCAAGGCATCAGCCCAGCTGGAAACGATCAAAACGAAAGCTGGTGAAACGTTCGGt                                        <  2:2579570/97‑1 (MQ=255)
                                                       cGACCAGGTTGATCAGGAAGTTGAACGTTTTCTGAGCGGTCGGGCCAAGGCATCAGCCCAGCTGGAAACGATCAAAACGAAAGCTGGTGAAACGTTCGGt                                        >  1:487116/1‑100 (MQ=255)
                                                        gACCAGGTTGATCAGGAAGTTGAACGTTTTCTGAGCGGTCGGGCCAAGGCATCAGCCCAGCTGGAAACGATCAAAACGAAAGCTGGTGAAACGTTCGGTg                                       <  2:595768/100‑1 (MQ=255)
                                                         aCCAGGTTGATCAGGAAGTTGAACGTTTTCTGAGCGGTCGGGCCAAGGCATCAGCCCAGCTGGAAACGATCAAAACGAAAGCTGGTGAAACGTTCGGTg                                       <  1:2935871/99‑1 (MQ=255)
                                                            aGGTTGATCAGGAAGTTGAACGTTTTCTGAGCGGTCGGGCCAAGGCATCAGCCCAGCTGGAAACGATCAAAACGAAAGCTGGTGAAACGTTCGGTgaaga                                   <  1:348435/100‑1 (MQ=255)
                                                            aGGTTGATCAGGAAGTTGAACGTTTTCTGAGCGGTCGGGCCAAGGCATCAGCCCAGCTGGAAACGATCAAAACGAAAGCTGGTGAAACGTTCGGTgaaga                                   <  1:2696845/100‑1 (MQ=255)
                                                             ggTTGATCAGGAAGTTGAACGTTTTCTGAGCGGTCGGGCCAAGGCATCAGCCCAGCTGGAAACGATCAAAACGAAAGCTGGTGAAACGTTCGGTgaagaa                                  >  1:22672/1‑100 (MQ=255)
                                                             ggTTGATCAGGAAGTTGAACGTTTTCTGAGCGGTCGGGCCAAGGCATCAGCCCAGCTGGAAACGATCAAAACGAAAGCTGGTGAAACGTTCGGTgaagaa                                  >  1:22827/1‑100 (MQ=255)
                                                             atttcaTCAGGAAGTTAAACATTATCTAAGCGGTCGGGCCAAGACATCTTCCCAGCTGTCAACAATAACAACGAAAGCTGGTGAAACGTTCGGTgaagaa                                  >  2:683087/6‑100 (MQ=255)
                                                               ttGATCAGGAAGTTGAACGTTTTCTTAGCGGTCGGGCCAAGGCATCAGCCCAGCTGGAAACGATCAAAACGAAAGCTGGTGAAACGTTCGGTGAAGaaaa                                <  1:1162461/100‑1 (MQ=255)
                                                                tGATCAGGAAGTTGAACGTTTTCTGAGCGGTCGGGCCAAGGCATCAGCCCAGCTGGAAACGATCAAAACGAAAGCTGGTGAAACGTTCGGTGAAGaaaaa                               <  1:2500949/100‑1 (MQ=255)
                                                                tGATCAGGAAGTTGAACGTTTTCTGAGCGGTCGGGCCAAGGCATCAGCCCAGCTGGAAAAGATCAAAACGAAAGCTGGTGAAACGTTCGGTGAAGaaaaa                               >  1:2323697/1‑100 (MQ=255)
                                                                 gATCAGGAAGTTGAACGTTTTCTGAGCGGTCGGGCCAAGGCATCAGCCCAGCTGGAAACGATCAAAACGAAAGCTGGTGAAACGTTCGGTGAAGAAAaag                              >  1:2571544/1‑100 (MQ=255)
                                                                 gATCAGGAAGTTGAACGTTTTCTGAGCGGTCGCGCCAAGGCATCAGCCCAGCTGGAAACGATCAAAACAAAAGCTGGTGAAACGTTCGGTGAAGAAAaag                              >  1:1042180/1‑100 (MQ=255)
                                                                   tCAGGAAGTTGAACGTTTTCTGAGCGGTCGGGCCAAGGCATCAGCCCAGCTGGAAACGATCCAAACGCAAGCTGGTCAAACGTTCGGTGAAGAAAaagaa                            >  2:852950/1‑100 (MQ=255)
                                                                    cAGGAAGTTGAACGTTTTCTGAGCGGTCGGGCCAAGGCATCAGCCCAGCTGGAAACGATCAAAACGAAAGCTTGTGAAACGTTCGGTGAAGAAAaagaag                           <  2:2315722/100‑1 (MQ=255)
                                                                    cAGGAAGTTGAACGTTTTCTGAGCGGTCGGGCCAAGGCATCAGCCCAGCTGGAAACGATCAAAACGAAAGCTGGTGAAACGTTCGGTGAAGAAAaagaag                           >  2:1235763/1‑100 (MQ=255)
                                                                     aGGAAGTTGAACGTTTTCTGAGCGGTCGGGCCAAGGCATCAGCCCAGCTGGAAACGATCAAAACGAAAGCTGGTGAAACGTTCGGTGAAGAAAAAGAAGc                          >  2:924911/1‑100 (MQ=255)
                                                                      ggAAGTTGAACGTTTTCTGAGCGGTCGGGCCAAGGCATCAGCCCAGCTGGAAATGATCAAAACGAAAGCTGGTGAAACGTTCGGTGAAGAAAAAGAAGcc                         <  2:1681630/100‑1 (MQ=255)
                                                                      ggAAGTTGAACGTTTTCTGAGCGGTCGGGCCAAGGCATCAGCCCAGCTGGAAACGATCAAAACGAAAGCTGGTGAAACGTTCGGTGAAGAAAAAGAAGcc                         >  2:1796706/1‑100 (MQ=255)
                                                                      ggAAGTTGAACGTTTTCTGAGCGGTCGGGCCAAGGCATCAGCCCAGCTGGAAACGATCAAAACGAAAGCTGGTGAAACGTTCGGTGAAGAAAAAGAAGcc                         <  1:1427934/100‑1 (MQ=255)
                                                                        aaGTTGAACGTTTTCTGAGCGGTCGGGCCAAGGCATCAGCCCAGCTGGAAACGATCAAAACGAAAGCTGGTGAAACGTTCGGTGAAGAAAAAGAAGCCAt                       >  2:1061489/1‑100 (MQ=255)
                                                                            tGAACGTTTTCTGAGCGGTCGGGCCAAGGCATCAGCCCAGCTGGAAACGATCAAAACGAAAGCTGGTGAAACGTTCGGTGAAGAAAAAGAAGCCATCttt                   >  1:232781/1‑100 (MQ=255)
                                                                             gAACGTTTTCTGAGCGGTCGGGCCAAGGCATCAGCCCAGCTGGAAACGATCAAAACGAAAGCTGGTGAAACGTTCGGTGAAGAAAAAGAAGCCATCTTTg                  >  2:2285993/1‑100 (MQ=255)
                                                                               aCGTTTTCTGAGCGGTCGGGCCAAGGCATCAGCCCAGCTGGAAACGATCAAAACGAAAGCTGGTGAAACGTTCGGTGAAGAAAAAGAAGCCATCTTTGaa                >  1:2551565/1‑100 (MQ=255)
                                                                                cGTTTTCTGAGCGGTCGGGCCAAGGCATCAGCCCAGCTGGAAACGATCAAAACGAAAGCTGGTGAAACGTTCGGTGAAGAAAAAGAAGCCATCTTTGAAg               >  2:1281954/1‑100 (MQ=255)
                                                                                   tttCTGAGCGGTCGGGCCAAGGCATCAGCCCAGCTGGAAACGATCAAAAGGAAAGATGGTGAAACGTTAGGTGAAGAAAAAGAAGCAATCTTTGa                 >  1:2550260/1‑95 (MQ=255)
                                                                                   tttCTGAGCGGTCGGGCCAAGGCATCAGCCCAGCTGGAAACGATCAAAACGAAAGCTGGTGAAACGTTCGGTGAAGAAAAAGAAGCCATCTTTGAAGGGc            >  1:2396878/1‑100 (MQ=255)
                                                                                      cTGAGCGGTCGGGCCAAGGCATCAGCCCAGCTGGAAACGATCAAAACGAAAGCTGGTGAAACGTTCGGTGAAGAAAAAGAAGCCATCTTTGAAGGGCata         >  2:2066851/1‑100 (MQ=255)
                                                                                        gAGCGGTCGGTCCAAGGCATCAGCCCAGCTGGAAACGATCAAAATGAAAGCTGTTGAAACGTTCGGTGAAGAAAAAGAAGCCATCTTTGAAGGGCatat        <  1:2577513/99‑1 (MQ=255)
                                                                                        gAGCGGTCGGGCCAAGGCATCAGCCCAGCTGGAAACGATCAAAACGAAAGCTGGTGAAACGTTCGGTGAAGAAAAAGAAGCCATCTTTGAAGGGCAtatt       >  1:1578811/1‑100 (MQ=255)
                                                                                        gAGCGGTCGGGCCAAGGCATCAGCCCAGCTGGAAACGATCAAAACGAAAGCTGGTGAAACGTTCGGTGAAGAAAAAGAAGCCATCTTTGAAGGGCAtatt       >  1:1543419/1‑100 (MQ=255)
                                                                                          gCGGTCGGGCCAAGGCATCAGCCCAGCTGGAAACGATCAAAACGAAAGCTGGTGAAACGTTCGGTGAAGAAAAAGAAGCCATCTTTGAAGGGCAtattat     <  1:2288281/100‑1 (MQ=255)
                                                                                           cGGTCGGGCCAAGGCATCAGCCCAGCTGGAAATGATCAAAACGAAAGCTGGTGAAACGTTCGGTGAAGAAAAAGAAGCCATCTTTGAAGGGCAtatta      <  1:1604681/98‑1 (MQ=255)
                                                                                           cGGTCGGGCCAAGGCATCAGCCCAGCTGGAAACGATCAAAACGAAAGCTGGTGAAACGTTCGGTGAAGAAAAAGAAGCCATCTTTGAAGGGCATATTAtg    >  1:1963297/1‑100 (MQ=255)
                                                                                            ggTCGGGCCAAGGCATCAGCCCAGCTGGAAACGATCAAAACGAAAGCTGGTGAAACGTTCGGTGAAGAAAAAGAAGCCATCTTTGAAGGGCATATTAtgc   <  2:2644005/100‑1 (MQ=255)
                                                                                            ggTCGGGCCAAGGCATCAGCCCAGCTGGAAACGATCAAAACGAAAGCTGGTGAAACGTTCGGTGAAGAAAAAGAAGCCATCTTTGAAGGGCATATTAtgc   <  2:1543401/100‑1 (MQ=255)
                                                                                            ggTCGGGCCAAGGCATCAGCCCAGCTGGAAACGATCAAAACGAAAGCTGGTGAAACGTTCGGTGAAGAAAAAGAAGCCATCTTTGAAGGGCATATTAtgc   >  1:2554978/1‑100 (MQ=255)
                                                                                            ggTCGGGCCAAGGCATCAGCCCAGCTGGAAACGATCAAAACGAAAGCTGGTGAAACGTTCGGTGAAGAAAAAGAAGCCATCTTTGAAGGGCATATTAtgc   >  1:886918/1‑100 (MQ=255)
                                                                                             gTCGGGCCAAGGCATCAGCCCAGCTGGAAACGATCAAAACGAAAGCTGGTGAAACGTTCGGTGAAGAAAAAGAAGCCATCTTTGAAGGGCATATTAtgct  >  1:40607/1‑100 (MQ=255)
                                                                                             gTCGGGCCAAGGCATCAGCCCAGCTGGAAACGATCAAAACGAAAGCTGGTGAAACGTTCGGTGAAGAAAAAGAAGCCATCTTTGAAGGGCATATTAtgct  >  2:985427/1‑100 (MQ=255)
                                                                                                 |                                                                                               
AAGCTCTGCTTCTGAAAGAAGACGAAATTGTCATTGACCGGAAAAAAATTTCTGCCGACCAGGTTGATCAGGAAGTTGAACGTTTTCTGAGCGGTCGTGCCAAGGCATCAGCCCAGCTGGAAACGATCAAAACGAAAGCTGGTGAAACGTTCGGTGAAGAAAAAGAAGCCATCTTTGAAGGGCATATTATGCT  >  NC_000913/2534109‑2534301

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: