Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,560,321 C→T 15.6% A22V (GCT→GTT)  yffL → CPZ‑55 prophage; uncharacterized protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,560,3210CT15.6% 256.0 / 34.9 109A22V (GCT→GTT) yffLCPZ‑55 prophage; uncharacterized protein
Reads supporting (aligned to +/- strand):  ref base C (42/50);  new base T (11/6);  total (53/56)
Fisher's exact test for biased strand distribution p-value = 1.90e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.39e-02

Only 100 of 116 total aligned reads displayed.

AATAAATTATTCAATATCAAGAACTTAAATCCATGTTTGCAACGAAAGATCCAGAGTTCGAAAATAGGATCAACACAAATAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCATGTCTGATATGCTAGCAGTAGAACAAGAAACAAACAATGATGTTCGTCA  >  NC_000913/2560225‑2560420
                                                                                                |                                                                                                   
aataatTTATTCAATATCAAGAACTTAAATACATGTTTGCAACTAAAGATCCAGAGTTCGAAAATAGGATCAACACAAATAAAAGCCCCCGGAAtgctgn                                                                                                  <  2:908826/100‑2 (MQ=255)
aaTAAATTATTCAATATCAAGAACTTAAATCCATGTTTGCAACGAAAGATCCAGAGTTCGAAAATAGGATCAACACAAATAAAAGCCCCCGGAAtgctgn                                                                                                  <  2:2753134/100‑2 (MQ=255)
aaTAAATTATTCAATATCAAGAACTTAAATCCATGTTTGCAACGAAAGATCCAGAGTTCGAAAATAGGATCAACACAAATAAAAGCCCCCGGAAtgctgc                                                                                                  <  1:598868/100‑1 (MQ=255)
aaTAAATTATTCAATATCAAGAACTTAAATCCATGTTTGCAACGAAAGATCCAGAGTTCGAAAATAGGATCAACACAAATAAAAGCCCCCGGAAtgctgc                                                                                                  >  2:46468/1‑100 (MQ=255)
aaTAAATTATTCAATATCAAGAACTTAAATCCATGTTTGCAACGAAAGATCCAGAGTTCGAAAATAGGATCAACACAAATAAAAGCCCCCGGAAtgctg                                                                                                   <  1:1402316/99‑1 (MQ=255)
 aTAAATTATTCAATATCAAGAATTTAATTCCATGTTTGCAACGAAAGATCCAGAGTTCGAAAATAGGATCAACACAAATAAAAGCCCCCGGAATGCTGCa                                                                                                 <  1:1381164/100‑1 (MQ=255)
 aTAAATTATTCAATATCAAGAACTTAAATCCATGTTTGCAACGAAAGATCCAGAGTTCGAAAATAGGATCAACACAAATAAAAGCCCCCGGAATGCTGCa                                                                                                 >  2:95124/1‑100 (MQ=255)
 aTAAATTATTCAATACCAAGAACTTAAATCCATGTTTGCAACGAAAGATCCAGAGTTCGAAAATAGGATCAACACAAATAAAAGCCCCCGGAATGCTGCa                                                                                                 <  1:2996163/100‑1 (MQ=255)
   aaaTTATTCAATATTATTAATTTAAATTCGTGTTTGCAACGAAAGATCAAGAGTTCGAAAATAGTATCAACACAAATAACAGCCCCCGGAATGCTGCAAc                                                                                               <  1:1305950/100‑1 (MQ=255)
   aaaTTATTCAATATCAAGAACTTAAATCCATGTTTGCAACGAAAGATCCAGAGTTCGAAAATAGGATCAACACAAATAAAAGCCCCCGGAATGCTGCAAc                                                                                               <  1:2837546/100‑1 (MQ=255)
   aaaTTATTCAATATCAAGAACTTAAATCCATGTTTGCAACGAAAGATCCAGAGTTCGAAAATAGGATCAACACAAATAAAAGCCCCCGGAATGCTGCAAc                                                                                               <  2:2079778/100‑1 (MQ=255)
   aaaTTATTCAATATCAAGAACTTAAATCCATGTTTGCAACGAAAGATCCAGAGTTCGAAAATAGGATCAACACAAATAAAAGCCCCCGGAATGCTGCAAc                                                                                               <  2:2773059/100‑1 (MQ=255)
    aaTTATTCAATATCGAGAACTTAAATCCATGTTTGCAACGAAAGATCCAGAGTTCGAAAATAGGATCAACACAAATAAAAGCCCCCGGAATGCTGCAAc                                                                                               <  1:3006095/99‑1 (MQ=255)
    aaTTATTCAATATCAAGAACTTAAATCCATGTTTGCAACGAAAGATCCAGAGTTCGAAAATAGGATCAACACAAATAAAAGCCCCCGGAATGCTGCAACa                                                                                              <  2:2371318/100‑1 (MQ=255)
     attattCAATATCAAGAACTTAAATCCATGTTTGCAACGAAAGATCCAGAGTTCGAAAATAGGATCAACACAAATAAAAGCCCCCGGAATGCTGCAACAt                                                                                             >  1:11081/1‑100 (MQ=255)
         ttCAATATCAAGAACTTAAATCCATGTTTGCAACGAAATATCCATAGTGCGAAAATAGGATCAACACAAATAAAAGCCCCCGGAATGCTGCAACATGCCg                                                                                         >  2:605382/1‑100 (MQ=255)
           cAATATCAAGAACTTAAATCCATGTTTGCAACGAAAGATCCAGAGTTCGAAAATAGGATCAACACAAATAAAAGCCCCCGGCATGTTGCAGCATTCCggg                                                                                       <  2:71226/100‑1 (MQ=38)
           cAATATCAAGAACTTAAATCCATGTTTGCAACGAAAGATCCAGAGTTCGAAAATAGGATCAACACAAATAAAAGCCCCCGGAATGCTGCAACATGCCggg                                                                                       <  1:713434/100‑1 (MQ=255)
            aaTATCAAGAACTTAAATCCATGTTTGCAACGAAAGATCCAGAGTTCGAAAATAGGATCAACACAAATAAAAGCCCCCGGAATGCTGCAACATGCCgggg                                                                                      <  1:2639775/100‑1 (MQ=255)
             atatCAAGAACTTAAATCCATGTTTGCAACGAAAGATCCAGAGTTCGAAAATAGGATCAACACAAATAAAAGCCCCCGGCATGTTGCAGCATTCCggggg                                                                                     <  2:1452196/100‑1 (MQ=38)
             atatCAAGAACTTAAATCCATGTTTGCAACGAAAGATCCAGAGTTCGAAAATAGGATCAACACAAATAAAAGCCCCCGGCATGTTGCACCATCCCggggg                                                                                     <  2:1580890/100‑1 (MQ=255)
              tatTAAGAACTTAAATCCATGTTTGCAACTAAAGATGCAGTGTTCGAAAATAGGATCAACACAAATAAACGCCCCCGGTATGCTGCAACATGCCGGGGGc                                                                                    <  2:2197576/100‑1 (MQ=255)
              tatCAAGAACTTAAATCCATGTTTGCATCGAAAGATCCAGAGTTCGAAAATAGGATCAACACAAATAAAAGCCCCCGGCATGTTGCAGCATTCCgggg                                                                                      <  2:1482719/98‑1 (MQ=38)
              tatCAAGAACTTAAATCCATGTTTGCAACGAAAGATCCAGAGTTCGAAAATAGGATCAACACAAATAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGc                                                                                    <  1:2608539/100‑1 (MQ=255)
              tatCAAGAACTTAAATCCATGTTTGCAACGAAAGATCCAGAGTTCGAAAATAGGATCAACACAAATAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGc                                                                                    <  2:2760649/100‑1 (MQ=255)
                tCAAGAACTTAAATCCATGTTTGCAACGAAAGATCCAGAGTTCGAAAATAGGATCAACACAAATAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCgg                                                                                  <  2:878116/100‑1 (MQ=255)
                tCAAGAACTTAAATCCATGTTTGCAACGAAAGATCCAGAGTTCGAAAATAGGATCAACACAAATAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCgg                                                                                  >  2:226519/1‑100 (MQ=255)
                tCAAGAACTTAAATCCATGTTTGCAACGAAAGATCCAGAGTTCGAAAATAGGATCAACACAAATAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCgg                                                                                  >  2:2215069/1‑100 (MQ=255)
                tCAAGAACTTAAATCCATGTTTGCAACGAAAGATCCAGAGTTCGAAAATAGGATCAACACAAATAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCgg                                                                                  >  1:16201/1‑100 (MQ=255)
                tCAAGAACTTAAATCCATGTTTGCAACGAAAGATCCAGAGTTCGAAAATAGGATCAACACAAATAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCgg                                                                                  <  1:1671843/100‑1 (MQ=255)
                 cAAGAACTTAAATCCATGTTTGCAACGAAAGATCCATATTTCGAAAATAGGATCAACACAAATAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCGGt                                                                                 <  1:773013/100‑1 (MQ=255)
                 cAAGAACTTAAATCCATGTTTGCAACGAAAGATCCAGAGTTCGAAAATAGGATCAACACAAATAAAAGCCCCCGGCATGTTGCAGCATTCCGGGGGcttt                                                                                 <  1:1186561/100‑4 (MQ=37)
                 cAAGAACTTAAATCCATGTTTGCAACGAAAGATCCAGAGTTCGAAAATAGGATCAACACAAATAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCgg                                                                                  >  2:1884766/1‑99 (MQ=255)
                  aaGAACTTAAATCCATGTTTGCAACGAAAGATCCAGAGTTCGAAAATAGGATCAACACAAATATAAGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTa                                                                                <  1:2139459/100‑1 (MQ=255)
                  aaGAACTTAAATCCATGTTTGCAACGAAAGATCCAGAGTTCGAAAATAGGATCAACACAAATAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTa                                                                                <  2:2105753/100‑1 (MQ=255)
                   aGATCTTAAATCCATGTTTGCAACGAAATTTCTAGAGTTCGAAAATAGGATCAACACAAATAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTa                                                                                <  1:768886/99‑1 (MQ=255)
                    gAACTTAAATCCATGTTTGCAACGAAAGATCCAGAGTTCGAAAATAGGATCAACACAAATAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTATg                                                                              <  2:207440/100‑1 (MQ=255)
                     aaCTTAAATCCATGTTTGCAACGAAAGATCCAGAGTTCGAAAATAGGATCAACACAAATAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTATga                                                                             <  2:963513/100‑1 (MQ=255)
                      aCTTAAATCCATGTTTGCAACGAAAGATCCAGAGTTCGAAAATAGGATCAACACAAATAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTATgag                                                                            <  2:2807323/100‑1 (MQ=255)
                       cTTAAATCCATGTTTGCAACGAAAGATCCAGAGTTCGAAAATAGGATCAACACAAATAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTATgaga                                                                           <  2:1897239/100‑1 (MQ=255)
                       cTTAAATCCATGTTTGCAACGAAAGATCCAGAGTTCGAAAATAGGATCAACACAAATAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTATgaga                                                                           <  1:975403/100‑1 (MQ=255)
                         tAAATCCATGTTTGCAACGAAAGATCCAGAGTTCGAAAATAGGATCAACACAAATAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTATGAGaaa                                                                         <  1:2107541/100‑1 (MQ=255)
                         tAAATCCATGTTTGCAACGAAAGATCCAGAGTTCGAAAATAGGATCAACACAAATAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTATGAGaa                                                                          <  1:2289207/99‑1 (MQ=255)
                         tAAATCCATGTTTGCAACGAAAGATCCAGAGTTCGAAAATAGGATCAACACAAATAAAAGCCCCAGGAATGCTGCAACATGCCGGGGGCGGTATGAGaaa                                                                         >  1:1125525/1‑100 (MQ=255)
                           aaTCCATGTTTGCAACGAAAGATCCAGAGTTCGAAAATAGGATCAACACAAATAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACa                                                                       >  2:2467269/1‑100 (MQ=255)
                             tCCATGTTTGCAACGAAAGATCCAGAGTTCGAAAATAGGATCAACACAAATAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACAAg                                                                     <  1:419999/100‑1 (MQ=255)
                              ccATGTTTGCAACGAAAGCTCCAGAGTTCGAAAATAGGATCAACTCAAATAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACaa                                                                      >  1:159640/1‑98 (MQ=255)
                               cATGTTTGCAACGAAAGATCCAGAGTTCGAAAATAGGATCAACACAAATAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGc                                                                    >  1:1539497/1‑99 (MQ=255)
                               cATGTTTGCAACGAAAGATCCAGAGTTCGAAAATAGGATCAACACAAATAAAAGCCCCCGGAATGCTGCAACATGCAAGGGGCGATATGAGAAACAAg                                                                     >  1:2729343/1‑98 (MQ=255)
                                aTGTTTGCAACGAAAGATCCAGAGTTCGAAAATAGGATCAACACAAATAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGCTa                                                                  <  2:1341916/100‑1 (MQ=255)
                                   tttGCAACGAAAGATCCAGAGTTCGAAAATAGGATCAACACAAATAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAg                                                               <  1:559575/100‑1 (MQ=255)
                                        aaCGAAAGATCGAGAGTTCGAAAATAGGAGCAACACAAATAAAAGGCCCCGGAATGCTGCAAAATGCCGGGGGCGGTATGAGAAACAAGCTAAAGgag                                                            <  2:829086/98‑1 (MQ=255)
                                        aaCGAAAGATCCAGAGTTCGAAAATAGGATCAACACAAATAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGaa                                                          >  1:1752410/1‑100 (MQ=255)
                                        aaCGAAAGATCCAGAGTTCGAAAATAGGATCAACACAAATAAAAGCACCCGGAATGCTGAAACATGACGGGGGCGGTATGAGAAACAAGCTAAAGGAGaa                                                          >  1:97838/1‑100 (MQ=255)
                                              aGATCCAGAGTTCGAAAATAGGATCAACACAAATAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTtctc                                                    <  1:1800455/100‑1 (MQ=255)
                                               gATCCAGAGTTCGAAAATAGGATCAACACAAATAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCa                                                   >  1:2713404/1‑100 (MQ=255)
                                               gATCCAGAGTTCGAAAATAGGATCAACACAAATAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCa                                                   >  2:116937/1‑100 (MQ=255)
                                                 tCCAGAGTTCGAAAATAGGATCAACACAAATAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCATg                                                 <  1:2796150/100‑1 (MQ=255)
                                                   cAGAGTTCGAAAATAGGATCAACACAAATAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCATGTc                                               >  2:1129036/1‑100 (MQ=255)
                                                       gTTCGAAAATAGGATCAACACAAATAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCATGTCTGat                                           >  1:1691371/1‑100 (MQ=255)
                                                       gTTCGAAAATAGGATCAACACAAATAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCATGTCTGat                                           >  1:1864520/1‑100 (MQ=255)
                                                       gTTAGAAAATAGGATCAACACAAATAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGTGAATTTCTCATGTCTGat                                           <  2:1187524/100‑1 (MQ=255)
                                                         tCGAAAATAGGATCAACACAAATAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCATGTCTGatat                                         <  1:2614471/100‑1 (MQ=255)
                                                          cGAAAATAGGATCAACACAAATAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCATGTCTGATATg                                        >  1:147793/1‑100 (MQ=255)
                                                          cGAAAATAGGATCAACACAAATAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCATGTCTGATATg                                        >  2:2029065/1‑100 (MQ=255)
                                                            aaaaTAGGATCAACACAAATAAAAGCCCCCGGAATGCTGCAACATGCCGGGGACGATATGAGAAAAAAAAAAAAGGAGAATTTCTCATGTCTGATATGc                                       >  1:769607/1‑99 (MQ=255)
                                                             aaaTAGGATCAACACAAATAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCATGTCTGATATGCTa                                     <  1:208521/100‑1 (MQ=255)
                                                              aaTAGGATCAACACAAATAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCATGTCTGATATGCTAg                                    >  1:973332/1‑100 (MQ=255)
                                                              aaTAGGATCAACACAAATAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCATGTCTGATATGCTAg                                    <  1:658077/100‑1 (MQ=255)
                                                              aaTAGGATCAACACAAATAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCATGTCTGATATGCTAg                                    <  1:1193736/100‑1 (MQ=255)
                                                               aTAGGATCAACACAAATAAAAGCCCCCGGAATGCTGTAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCATGTCTGATATGCTAGc                                   <  1:2573482/100‑1 (MQ=255)
                                                                 aGGATCAACACGAATAAAATCCCCCGGAATGCTGCAACATTCCGTGGGCGGTATGAGAAACAAGCTAAAGGATAATTTCTCATGTCTGATATGCTAGc                                   <  2:2876688/98‑1 (MQ=255)
                                                                    aTCAACACAAATAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGCATTTCTCATGTCTGATATGCTAGCAGTa                               >  2:2573164/1‑99 (MQ=255)
                                                                     tCAACACAAATAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCATGTCTGATATGCTAGCAGTAGa                             <  1:149960/100‑1 (MQ=255)
                                                                        acacAAATAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCATGTCTGATATGCTAGCAGTAGAACa                          >  2:2553109/1‑100 (MQ=255)
                                                                           tctcataccgCCCCCGGCATGTTGCAGCATTCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCATGTCTGATATGCTAGCAGTAGAACAAGa                       >  2:1390927/10‑100 (MQ=25)
                                                                           caAATAAAAGCCCCTGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCATGTCTGATATGCTAGCAGTAGAACAAGa                       >  2:1635763/1‑100 (MQ=255)
                                                                            aaaTAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCATGTCTGATATGCTAGCAGTAGAACAAGaa                      <  1:226521/100‑1 (MQ=255)
                                                                              atctaccgccccGGCATGTTGCAGCATTCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCATGTCTGATATGCTAGCAGTAGAACAAGaaac                    <  1:258244/92‑1 (MQ=37)
                                                                                aaaaGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCATGTCTGATATGCTAGCAGTAGAACAAGaaacaa                  >  2:644025/1‑100 (MQ=255)
                                                                                  ccgCCCCCGGCATGTTGCAGCATTCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCATATCTGATATGATAGCAGTAGAACAAGaaacaaac                >  1:525121/3‑100 (MQ=37)
                                                                                  accgccccGGCATGTTGCAGCATTCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCATGTCTGATATGCTAGCAGTAGAACAAGaaacagat                >  2:258241/5‑97 (MQ=37)
                                                                                   cgCCCCCGGCATGTTGCAGCATTCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCATGTCTGATATGCTAGCAGTAGAACAAGAaacaaaca               >  2:1894762/2‑100 (MQ=38)
                                                                                    gCCCCCGGCATGTTGCAGCATTCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCATGTCTGATATGCTAGCAGTAGAACAAGAAacaaacaa              >  1:727572/1‑100 (MQ=38)
                                                                                    gCCCCCGGCATGTTGCAGCATTCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTATCATGTCAGATATGCTAGCAGTAGAACAAGAAacaaacaa              >  1:640718/1‑100 (MQ=37)
                                                                                     cccccGGCATGTTGCAGCATTCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCATGTCTGATATGCTAGCAGTAGAACAAGAAACAAACAat             >  2:2844295/1‑100 (MQ=38)
                                                                                     cccccGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCATGTCTGATATGCTAGCAGTAGAACAAGAAACAAACAat             >  2:2437344/1‑100 (MQ=255)
                                                                                     cccccGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCATGTCTGATATGCTAGCAGTAGAACAAGAAACAAACAat             <  1:1289291/100‑1 (MQ=255)
                                                                                      ccccGGCATGTTGCAGCATTCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCATGTCTGATATGCTAGCAGTAGAACAAGAAACAAACAatg            >  1:2937318/1‑100 (MQ=38)
                                                                                      ccccGGCATGTTGCAGCATTCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCATGTCTGATATGCTAGCAGTAGAACAAGAAACAAACAatg            >  2:2491012/1‑100 (MQ=38)
                                                                                      ccccGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCATGTCTGATATGCTAGCAGTAGAACAAGAAACAAACAatg            >  1:228540/1‑100 (MQ=255)
                                                                                       cccGGCATGTTGCAGCATTCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCATGTCTGATATGCTAGCAGTAGAACAAGAAACAAACAatga           >  1:1292819/1‑100 (MQ=38)
                                                                                       cccGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCATGTCTGATATGCTAGCAGTAGAACAAGAAACAAACAatga           >  2:491556/1‑100 (MQ=255)
                                                                                       cccGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCATGTCTGATATGCTAGCAGTAGAACAAGAAACAAACAatga           >  1:807134/1‑100 (MQ=255)
                                                                                       cccGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCATGTCTGATATGCTAGCAGTAGAACAAGAAACAAACAatga           >  2:854282/1‑100 (MQ=255)
                                                                                        ccGGCATGTTGCAGCATTCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCATGTCTGATATGCTAGCAGTAGAACAAGAAACAAACAatgat          >  2:1733185/1‑100 (MQ=38)
                                                                                        ccGGAATGCTGCAACCTGCCGGGAGAGGTATGAGAAACACGCTAACGGAGAATTCCTCATGTCTGATATGTTAGCAGTAGAACAAGaatcaaactg              <  2:1763573/96‑3 (MQ=255)
                                                                                        ccGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCATGTCTGATATGCTAGCAGTAGAACAAGAAACAAACAatgat          >  2:1521263/1‑100 (MQ=255)
                                                                                               gctgcAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCATGTCTGATATGCTAGCAGTAGAACAAGAAACAAACAATGATGTTCGTc   >  2:547955/1‑100 (MQ=255)
                                                                                                ctgcAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCATGTCTGATATGCTAGCAGTAGAACAAGAAACAAACAATGATGTTCGTCa  >  1:2944616/1‑100 (MQ=255)
                                                                                                |                                                                                                   
AATAAATTATTCAATATCAAGAACTTAAATCCATGTTTGCAACGAAAGATCCAGAGTTCGAAAATAGGATCAACACAAATAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCATGTCTGATATGCTAGCAGTAGAACAAGAAACAAACAATGATGTTCGTCA  >  NC_000913/2560225‑2560420

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: