BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	232,196	G→T	100%	G91C (GGC→TGC)	yafE →	putative S‑adenosyl‑L‑methionine‑dependent methyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	232,196	0	G	T	100.0%	428.7 / ‑6.7	132	G91C (GGC→TGC)	yafE	putative S‑adenosyl‑L‑methionine‑dependent methyltransferase
Reads supporting (aligned to +/- strand): ref base G (0/2); new base T (69/60); total (70/62)

Only 100 of 132 total aligned reads displayed.

GATAACGCATTTGATATTGTTATCAGCCGTTATTCTGCCCATCACTGGCATGATGTTGGTGCAGCACTGCGAGAAGTGAATAGGATATTGAAACCTGGCGGTAGGCTGATTGTGATGGACGTAATGTCTCCGGGTCACCCAGTGCGCGACATCTGGTTACAGACGGTAGAAGCATTACGCGATACCTCTCACG  >  NC_000913/232100‑232292

gATAACGCATTTGATATTGTTATCAGCCGTTATTCTGCCCATCACTGGCATGATTTTGGTGCAGCACTGCGAGAAGTGAATAGGATATTGAAACCTTGCg                                                                                               >  2:2011661/1‑100 (MQ=255)

gATAACGCATTTGATATTGTTATCAGCCGTTATTCTGCCCATCACTGGCATGATGTTGGTGCAGCACTGCGAGAAGTGAATAGGATATTGAAACCTTGCg                                                                                               >  1:1007164/1‑100 (MQ=255)

 aTAACGCATTTGATATTGTTATCAGCCGTTATTCTGCCCATCACTGGCATGATGTTGGTGCAGCACTGCGAGAAGTGAATAGGATATTGAAACCTTGCgg                                                                                              <  1:2122943/100‑1 (MQ=255)

    aCGCATTTGATATTGTTATCAGCCGTTATTCTGCCCATCACTGGCATGATGTTGGTGCAGCACTGCGAGAAGTGAATAGGATATTGAAACCTTGCg                                                                                               <  2:444720/96‑1 (MQ=255)

    aCGCATTTGATATTGTTATCAGCCGTTATTCTGCCCATCACTGGCATGATGTTGGTGCAGCACTGCGAGAAGTGAATAGGATATTGAAACCTTGCGGTAg                                                                                           >  1:2421645/1‑100 (MQ=255)

    aCGCATTTGATATTGTTATCAGCCGTTATTCTGCCCATCACTGGCATGATGTTGGTGCAGCACTGCGAGAAGTGAATAGGATATTGAAACCTTGCGGTAg                                                                                           >  1:629350/1‑100 (MQ=255)

     cGCATTTGATATTGTTATCAGCCGTTATTCTGCCCATCACTGGCATGATGTTGGTGCAGCACTGCGAGAAGTGAATAGGATATTGAAACCTTGCGGTAgg                                                                                          >  1:1470113/1‑100 (MQ=255)

     cGCATTTGATATTGTTATCAGCCGTTATTCTGCCCATCACTGGCATGATGTTGGTGCAGCACTGCGAGAAGTGAATAGGATATTGAAACCTTGCGGTAg                                                                                           <  1:2694352/99‑1 (MQ=255)

      gCATTTGCTATCGTTAGCAGCCGTTATCATGCCCATCCATGGCATGATGTTGGTGAAGTACTGCGACAAGTGAATAGCCCATTCAAACCTTGCCGTAGGc                                                                                         <  2:1765125/100‑1 (MQ=255)

      gCATTTGATATTGTTATCAGCCGTTATTCTGCCCATCACTGGCATGATGTTGGTGCAGCACTGCGAGAAGTGAATAGGATATTGAAACCTTGCGGTAGGc                                                                                         <  2:1793481/100‑1 (MQ=255)

        aTTTGATATTGTTATCAGCCGTTATTCTGCCCATCACTGGCATGATGTTGGTGCAGCACTGCGAGAAGTGAATAGGATATTGAAACCTTGCGGTAGGCTg                                                                                       >  1:465886/1‑100 (MQ=255)

         tttGATATTGTTATCAGCCGTTATTCTGCCCATCACTGGCATGATGTTGGTGCAGCACTGCGAGAAGTGAATAGGATATTGAAACCTTGCGGTAGGCTGa                                                                                      <  1:1554320/100‑1 (MQ=255)

           tGATATTGTTATCAGCCGTTATTCTGCCCATCACTGGCATGATGTTGGTGCAGCACTGCGAGAAGTGAATAGGATATTGAAACCTAGCGGTAGGCTGAtt                                                                                    >  2:2065904/1‑100 (MQ=255)

            gATATTGTTATCAGCCGTTATTCTGCCCATCACTGGCATGATGTTGGTGCAGCACTGCGAGAAGTTAATAGGATATTGAAACCTTGCGGTAGGCTGATtg                                                                                   <  2:2562502/100‑1 (MQ=255)

            gATATTGTTATCAGCCGTTATTCTGCCCATCACTGGCATGATGTTGGTGCAGCACTGCGAGAAGTGAATAGGATATTGAAACCTTGCGGTAGGCTGATtg                                                                                   <  2:2027477/100‑1 (MQ=255)

             atatTGTTATCAGCCGTTATTCTGCCCATCACTGGCATGATGTTGGTGCAGCACTGCGAGAAGTGAATATGATATTGAAACCTTGCGGTAGGCTGATtgt                                                                                  <  2:1663922/100‑1 (MQ=255)

             atatTGTTATCAGCCGTTATTCTGCCCATCACTGGCATGATGTTGGTGCAGCACTGCGAGAAGTGAATAGGATATTGAAACCTTGCGGTAGGCTGATtgt                                                                                  >  1:736436/1‑100 (MQ=255)

             atatTGTTATCAGCCGTTATTCTGCCCATCACTGGCATGATGTTGGTGCAGCACTGCGAGAAGTGAATAGGATATTGAAACATTGCGGTAGGCTGATtgt                                                                                  >  2:535623/1‑100 (MQ=255)

              tatTGTTATCAGCCGTTATTCTGCCCATCACTGGCATGATGTTGGTGCAGCACTGCGAGAAGTGAATAGGATATTGAAACCTTGCGGTAGGCTGATtgtg                                                                                 >  1:2399948/1‑100 (MQ=255)

               atTGTTATCAGCCGTTATTCTGCCCATCACTGGCATTATGTTGGTGCAGCACTGCGAGAAGTGAATAGGATATTGAAACCTTGCGGTAGGCTGATTGTGa                                                                                <  1:61904/100‑1 (MQ=255)

                ttGTTATCAGCTGTTATTCTGCCCATCACTGGCATGATGTTGGTGCAGCACTGCGAGAAGTGAATAGGATATTGAAACCTTGCGGTAGGCTGATTGTGAt                                                                               <  1:2927968/100‑1 (MQ=255)

                ttGTTATCAGCCGTTATTCTGCCCATCACTGGCATGATGTTGGTGCAGCACTGCGAGAAGTGAATAGGATATTGAAACCTTGCGGGAGGCTGATTGTGAt                                                                               >  2:2317988/1‑100 (MQ=255)

                 tGTTATCAGCCGTTATTCTGCCCATCACTGGCATTATGTTGGTGCAGCACTGCGAGAAGTGAATAGGATATTGAAACCTTGCGGTAGGCTGATTGTGATg                                                                              <  2:2511670/100‑1 (MQ=255)

                  gTTATCAGCCGTTATTCTGCCCATCACTGGCATGATGTTGGTGCAGCACTGCGAGAAGTGAATAGGATATTGAAACCTTGCGGTAGGCTGATTGTGATgg                                                                             <  2:1138321/100‑1 (MQ=255)

                    tATCAGCCGTTATTCTGCCCATCACTGGCATGATGTTGGTGCAGCACTGCGAGAAGTGAATAGGATATTGAAACCTTGCGGTAGGCTGATTGTGATGGAc                                                                           >  2:2049641/1‑100 (MQ=255)

                     aTCAGCCGTTATTGTGCCAATCACGGGCATAATTATATCACAGAACTGAGGGACGTGAATAGGATATTAAAACACTGAGGTAGGCCGCTTGTGACAGACg                                                                          >  2:1120822/1‑100 (MQ=255)

                     aTCAGCCGTTATTCTGCCCATCACTGGCATGATGTTTGTTCATCTCTGCGAGAAGTGAATAGGATATTGAAACCTTGCGGTAGGCTGATTGTGATGGACg                                                                          <  1:159264/100‑1 (MQ=255)

                         gCCGTTATTCTGCCCATCACTGGCATGATGTTGGTGCAGCACTGCGAGAAGTGAATAGGATATTGAAACCTTGCGGTAGGCTGATTGTGATGGACGTAAt                                                                      >  1:93722/1‑100 (MQ=255)

                          ccGTTATTCTGCCCATCACTGGCATGATGTTGGTGCAGCACTGCGAGAAGTGAATAGGATATTGAAACCTTGCGGTAGGCTGATTGTGATGGACGTAATg                                                                     <  2:93721/100‑1 (MQ=255)

                          ccGTTATTCTGCCCATCACTGGCATGATGTTGGTGCAGCACTGCGAGAAGTGAATAGGATATTGAAACCTTGCGGTAGGCTGATTGTGATGGACGTAATg                                                                     <  1:1646255/100‑1 (MQ=255)

                          ccGTTATTCTGCCCATCACTGGCATGATGTTGGTGCAGCACTGCGAGAAGTGAATAGGATATTGAAACCTGGCGGTAGGCTGATTGTGATGGACGTAATg                                                                     <  2:860823/100‑1 (MQ=255)

                           cGTTATTCTGCCCATCACTGGCATGATGTTGGTGCAGCACTGCGAGAAGTGAATAGGATATTGAAACATTGCGGTAGGCTGATTGTGATGGACGTAATGt                                                                    >  1:218042/1‑100 (MQ=255)

                           cGTTATTCTGCCCATCAATGGCATGATGTTGGTGCAGCACTGCGAGAAGTGAATAGGATATTGAAACCTTGCGGTAGGCTGATTGTGATGGACGTAATg                                                                     <  1:2642235/99‑1 (MQ=255)

                            gTTATTCTGCCCATCACTGGCATGATGTTGGTGCAGCACTGCGAGAAGTGAATAGGATATTGAAACCTTGCGGTAGGCTGATTGTGATGGACGTAATGtc                                                                   <  1:2121767/100‑1 (MQ=255)

                                ttCTGCCCATCACTGGCATGATGTTGGTGCAGCACTGCGAGAAGTGAATAGGATATTGAAACCTTGCGGTAGGCTGATTGTGATGGACGTAATGTCTCCg                                                               <  1:1898590/100‑1 (MQ=255)

                                 tCTGCCCATCACTGGCATGATGTTGGTGCAGCACTGCGAGAAGTGAATAGGATATTGAAACCTTGCGGTAGGCTGATTGTGATGGACGTAATGTCTCCgg                                                              >  2:1172030/1‑100 (MQ=255)

                                  cTGCCCATCACTGGCATGATGTTGGTGCAGCACTGCGAGAAGTGAATAGGATATTGAAACCTTGCGGTAGGCTGATTGTGATGGACGTAATGTCTCCggg                                                             <  2:736432/100‑1 (MQ=255)

                                  cTGCCCATCACTGGCATGATGTTGGTGCAGCACTGCGAGAAGTGAATAGGATATTGAAACCTTGCGGTAGGCTGATTGTGATGGACGTAATGTCTCCggg                                                             <  1:1909386/100‑1 (MQ=255)

                                    gCCCATCACTGGCATGATGTTGGTGCAGCACTGCGAGAAGTGAATAGGATATTGAAACCTTGCGGTAGGCTGATTGTGATGGACGTAATGTCTCCGGGTc                                                           >  2:1721486/1‑100 (MQ=255)

                                    gCCCATCACTGGCATGATGTTGGTGCAGCACTGCGAGAAGTGAATAGGATATTGAAACCTTGCGGTAGGCTGATTGTGATGGACGTAATGTCTCCGGGTc                                                           >  1:1056026/1‑100 (MQ=255)

                                        atctcTGGCATGATGTTGGTGCAGCACTGCGAGAAGTGAATAGGATATTGAAACCTTGCGGTAGGCTGATTGTGATGGACGTAATGTCTCCGGGTCAccc                                                       <  2:652958/96‑1 (MQ=255)

                                        aTCACTGGCATGATGTTGGTGCAGCACTGCGAGAAGTGAATAGGATATTGAAACCTTGCGGTAGGCTGATTGTGATGGACGTAATGTCTCCGGGTCAccc                                                       <  2:326312/100‑1 (MQ=255)

                                           aCTGGCATGATGTTGGTGCAGCACTGCGAGAAGTGAATAGGATATTGAAACCTTGCGGTAGGCTGATTGTGATGGACGTAATGTCTCCGGGTCACCCAGt                                                    >  1:2719325/1‑100 (MQ=255)

                                            cTGGCATGATGTTGGTGCAGCACTGCGAGAAGTGAATAGGATATTGAAACCTTGCGGTAGGCTGATTGTGATGGACGTAATGTCTCCGGGTCACCCAgat                                                   >  1:652962/1‑98 (MQ=255)

                                            cTGGCATGATGTTGGTGCAGCACTGCGAGAAGTGAATAGGATATTGAAACCTTGCGGTAGGCTGATTGTGATGGACGTAATGTCTCCGGGTCACCCAGTg                                                   >  1:2016587/1‑100 (MQ=255)

                                            cTGGCATGATGTTGGTGCAGCACTGCGAGAAGTGAATAGGATATTGAAACCTTGCGGTAGGCTGATTGTGATGGACGTAATGTCTCCGGGTCACCCAGTg                                                   <  1:2104354/100‑1 (MQ=255)

                                              ggCATGATGTTGGTGCAGCACTGCGAGAAGTGAATAGGATATTGAAACCTTGCGGTAGGCTGATTGTGATGGACGTAATGTCTCCGGGTCACCCAGTgcg                                                 >  1:2982162/1‑100 (MQ=255)

                                              ggCATGATGTTGGTGCAGCACTGCGAGAAGTGAATAGGATATTGAAACCTTGCGGTAGGCTGATTGTGATGGACGTAATGTCTCCGGGTCACCCAGTgcg                                                 >  2:845369/1‑100 (MQ=255)

                                                cATGATGTTGGTGCAGCACTGCGAGAAGTGAATAGGATATTGAAACCTTGCGGTAGGCTGATTGTGATGGACGTAATGTCTCCGGGTCACCCAGTGcgcg                                               <  2:1126277/100‑1 (MQ=255)

                                                cATGATGTTGGTGCAGCACTGCGAGAAGTGAATAGGATATTGAAACCTTGCGGTAGGCTGATTGTGATGGACGTAATGTCTCCGGGTCACCCAGTGcgcg                                               <  1:156408/100‑1 (MQ=255)

                                                cATGATGTTGGTGCAGCACTGCGAGAAGTGAATAGGATATTGAAACCTTGCGGTAGGCTGATTGTGATGGACGTAATGTCTCCGGGTCACCCAGTGcgcg                                               >  2:149302/1‑100 (MQ=255)

                                                     tgTTGGTGCCGCACTGCGAGAAGTGAAAAGGATATTGAAACCTTTCGGTAGGCTGATTATGATGGCCGTAATGTCTCCGGGTCACCCAGTGCGCGACAt                                           >  2:1407828/1‑99 (MQ=255)

                                                     tgTTGGTGCAGCACTGCGAGAAGTGAATAGGATATTGAAACCTTGCGGTAGGCTGATTGTGATGGACGTAATGTCTCCGGGTCACCCAGTGCGCGACAt                                           <  2:1487938/99‑1 (MQ=255)

                                                      gTTGGTGCAGCACTGCGAGAAGTGAATAGGATATTGAAACCTTGCGGTAGGCTGATTGTGATGGACGTAATGTCTCCGGGTCACCCAGTGCGCGACATCt                                         <  1:1710240/100‑1 (MQ=255)

                                                        tGGTGCAGCACTGCGAGAAGTGAATAGGATATTGAAACCTTGCGGTAGGCTGATTGTGATGGACGTAATGTCTCCGGGTCACCCAGTGCGCGACATCTgg                                       <  2:661119/100‑1 (MQ=255)

                                                        tGGTGCAGCACTGCGAGAAGTGAATAGGATATTGAAACCTTGCGGTAGGCTGATTGTGATGGACGTAATGTCTCCGGGTCACCCAGTGCGCGACATCTg                                        >  2:2745282/1‑99 (MQ=255)

                                                         ggTGCAGCACTGCGAGAAGTGAATAGGATATTGAAACCTTGCGGTAGGCTGATTGTGATGGACGTAATGTCTCCGGGTCACCCAGTGCGCGACATCTGGt                                      >  1:924067/1‑100 (MQ=255)

                                                         ggTGCAGCACTGCGAGAAGTGAATAGGATATTGAAACCTTGCGGTAGGCTGATTGTGATGGACGTAATGTCTCCGGGTCACCCAGTGCGCGACATCTGGt                                      <  2:2765781/100‑1 (MQ=255)

                                                         atctcagcaCTGCGAGAAGTGAATAGGATATTGAAACCTTGCGGTAGGCTGATTGTGATGGACGTAATGTCTCCGGGTCACCCAGTGCGCGACATCTGGt                                      <  2:1731733/96‑1 (MQ=255)

                                                          gTGCAGCACTGCGAGAAGTGAATATGTTATTTAAATCTTGCGGTAGGCTGATTGTGATGGACGTAATGTCTCCGGGTCACCCAGTGCGCGACATCTGGt                                      <  1:771015/99‑1 (MQ=255)

                                                           tGCAGCACTGCGAGAAGTGAATAGGATATTGAAACCTTGCGGTAGGCTGATTGTGATGGACGTAATGTCTCCGGGTCACCCAGTGCGCGACATCTGGTTa                                    <  2:2986328/100‑1 (MQ=255)

                                                           tGCAGCACTGCGAGAAGTGAATAGGATATTGAAACCTTGCGGTAGGCTGATTGTGATGGACGTAATGTCTCCGGGTCACCCAGTGCGCGACATCTGGTTa                                    >  2:2346350/1‑100 (MQ=255)

                                                           tGCAGCACTGCGAGAAGTGAATAGGATATTGAAACCTTGCGGTAGGCTGATTGTGATGGACGTAATATCTCCGGGTCACCCAGTGCGCGAAATCTGGTTa                                    >  1:1207214/1‑100 (MQ=255)

                                                           tGCAGCACTGCGAGAAGTGAATAGGATATTGAAACCTTGCGGTAGGCTGATGGTGATGGACGTAATGTCTCCGGGTCACCCAGTGCGCGACATCTGGTTa                                    >  2:1323940/1‑100 (MQ=255)

                                                             cagcaCTGCGAGAAGTGAATAGGATATTGAAACCTTGCGGTAGGCTGATTGTGATGGACGTAATGTCTCCGGGTCACCCAGTGCGCGACATCTGGTTACa                                  <  1:2785970/100‑1 (MQ=255)

                                                              agcaCTGGGAGAAGGGAATAGGATATTGAGACCTTGCGGTAGGCTGATTGTGATGGACGTAATGTCTCCGGGTAACCCAGTGCGCGATATCTGGTTACAg                                 <  2:2543409/100‑1 (MQ=255)

                                                               gcaCTGCGAGAAGTGAATAGGATATTGAAACCTTGCGGTAGGCTGATTGTGATGGACGTAATGTCTCCGGGTCACCCAGTGCGCGACATCTGGTTACAGa                                >  2:693274/1‑100 (MQ=255)

                                                                caCTGCGAGAAGTGAATAGGATATTGAAACCTTGCGGTAGGCTGATTGTGATGGACGTAATGTCTCCGGGTCACCCAGTGCGCGACATCTGGTTACAGAc                               >  2:2712211/1‑100 (MQ=255)

                                                                  cggCGAGAAGTGAATAGGATATTGAAACCTTGCGGTAGGCTGATTGTGATGGACGTAATGTCTCCGGCTCTCCCAGTGCGCGACATCTGGTTACAg                                 <  2:1435043/94‑1 (MQ=255)

                                                                  cTGCGAGAAGTGAATAGGATATTGAAACCTTGCGGTAGGCTGATTGTGATGGACGTAATGTCTCCGGGTCACCCAGTGCGCGACATCTGGTTACAGACgg                             >  1:140892/1‑100 (MQ=255)

                                                                   tGCGAGAAGTGAATAGGATATTGAAACCTTGCGGTAGGCTGATTGTGATGGACGTAATGTCTCCGGGTCACCCAGTGCGCGACATCTGGTTACAGACGGt                            >  2:1340501/1‑100 (MQ=255)

                                                                   tGCGAGAAGTGAATAGGATATTGAAACCTTGCGGTAGGCTGATTGTGATGGACGTAATGTCTCCGGGTCACCCAGTGCGCGACATCTGGTTACAGACGGt                            <  2:2365715/100‑1 (MQ=255)

                                                                    gCGAGAAGTGAATAGGATATTGAAACCTTGCGGTAGGCTGATTGTGATGGACGTAATGTCTCCGGGTCACCCAGTGCGCGACATCTGGTTACAGACGGt                            <  2:888916/99‑1 (MQ=255)

                                                                    gCGAGAAGTGAATAGGATATTGAAACCTTGCGGTAGGCTGATTGTGATGGACGTAATGTCTCCGGGTCACCCAGTGCGCGACATCTGGTTACAGACGGTa                           >  1:2961127/1‑100 (MQ=255)

                                                                    gCGAGAAGTGAATAGGATATTGAAACCTTGCGGTAGGCTGATTGTGATGGACGTAATGTCTCCGGGTCACCCAGTGCGCGACATCTGGTTACAGACGGTa                           >  1:317180/1‑100 (MQ=255)

                                                                     cGAGAAGTGAATAGGATATTGAAACCTTGCGGTAGGCTGATTGTGATGGACGTAATGTCTCCGGGTCACCCAGTGCGCGACATCTGGTTACAGACGGTAg                          >  2:107123/1‑100 (MQ=255)

                                                                     cGAGAAGTGAATAGGATATTGAAACCTTGCGGTAGGCTGATTGTGATGGACGTAATGTCTCCGGGTCACCCAGTGCGCGACATCTGGTTACAGACGGTAg                          <  2:840683/100‑1 (MQ=255)

                                                                      gagaAGTGAATAGGATATTGAAACCTTGCGGTAGGCTGATTGTGATGGACGTAATGTCTCCGGGTCACCCAGTGCGCGACATCTGGTTACAGACGGTAGa                         <  1:1172041/100‑1 (MQ=255)

                                                                      gagaAGTGAATAGGATATTGAAACCTTGCGGTAGGCTGATTGTGATGGACGTAATGTCTCCGGGTCACCCAGTGCGCGACATCTGGTTACAGACGGTAGa                         >  1:2395185/1‑100 (MQ=255)

                                                                      gagaAGTGAATAGGATATTGAAACCTTGCGGTAGGCTGATTGTGATGGACGTAATGTCTCCGGGTCACCCAGTGCGCGACATCTGGTTACAGACGGTAGa                         >  2:1510713/1‑100 (MQ=255)

                                                                        gaAGTGAATAGGATATTGAAACCTTGCGGTAGGCTGATTGTGATGGACGTAATGTCTCCGGGTCACCCAGTGCGCGACATCTGGTTACAGACGGTAGAAg                       >  2:483091/1‑100 (MQ=255)

                                                                         aaGTGAATAGGATATTGAAACCTTGCGGTAGGCTGATTGTGATGGACGTAATGTCTCCGGGTCACCCAGTGCGCGACATCTGGTTACAGACGGTAGAAGc                      <  1:515926/100‑1 (MQ=255)

                                                                          aGTGAATAGGATATTGAAACCTTGCGGTAGGCTGATTGTGATGGACGTAATGTCTCCGGGTCACCCAGTGCGCGACATCTGGTTACAGACGGTAGAAGCa                     >  2:509786/1‑100 (MQ=255)

                                                                           gTGAATAGGATATTGAAACCTTGCGGTAGGCTGATTGTGATGGACGTAATGTCTCCGGGTCACCCAGTGCGCGACATCTGGTTACAGACGGTAGAAGCAt                    <  2:1109178/100‑1 (MQ=255)

                                                                           gTGAATAGGATATTGAAACCTTGCGGTAGGCTGATTGTGATGGACGTAATGTCTCCGGGTCACCCAGTGCGCGACATCTGGTTACAAACAGTAGAAGAAt                    >  1:2893734/1‑100 (MQ=255)

                                                                             gAATAGGATATTGAAACCTTGCGGTAGGCTGATTGTGATGGACGTAATGTCTCCGGGTCACCCAGTGCGCGACATCTGGTTACAGACGGTAGAAGCATTa                  <  1:2065927/100‑1 (MQ=255)

                                                                                tAGGATATTGAAACCTTGCGGTAGGCTGATTGTGATGGACGTAATGTCTCCGGGTCACCCAGTGCGCGTCATCTGGTTACAGACGGTAGAAGCATTActc               <  1:2047426/100‑3 (MQ=255)

                                                                                 aGGATATTGAAACCTTGCGGTAGGCTGATTGTGATGGACGCAATGTCTCCGGGTCACCCAGTGCGCGACATCTGGTTACAGACGGTAGAAGCATTAcgcg              <  1:408783/100‑1 (MQ=255)

                                                                                  ggATATTGAAACCTTGCGGTAGGCTGATTGTGATGGACGTAATGTCTCCGGGTCACCCAGTGCGCGACATCTGGTTACAGACGGTAGAAGCATTAcgcg              >  2:791926/1‑99 (MQ=255)

                                                                                     tatTGATACTTTGCGGTAGGCTGATTGTGATGGACGTAATGTCTCCGGGTCACCTAGTGCGCGTCGTCTGGTTACAGACGGTAGAAGTATTACGCGATaa          <  2:1237122/100‑2 (MQ=255)

                                                                                      atTGAAACCTTGCGGTAGGCTGATTGTGATGGACGTAATGTCTCCGGGTCACCCAGTGCGCGACATCTGGTTACAGACGGTAGAAGCATTACGCGATAcc         <  1:1327445/100‑1 (MQ=255)

                                                                                      atTGAAACCTTGCGGTAGGCTGATTGTGATGGACGTAATGTCTCCGGGTCACCCAGTGCGCGACATCTGGTTACAGACGGTAGAAGCATTACGCGATAcc         >  2:1954143/1‑100 (MQ=255)

                                                                                       ttGAAACCTTGCGGTAGGCTGATTGTGATGGACGTAATGTCTCCGGGTCACCCAGTGCGCGACATCTGGTTACAGACGGTAGAAGCATTACGCGATACct        >  2:1584028/1‑100 (MQ=255)

                                                                                       ttGAAACCTTGCGGTAGGCTGATTGTGATGGACGTAATGTCTCCGGGTCACCCAGTGCGCGACATCTGGTTACAGACGGTAGAAGCATTACGCGATACct        >  2:905828/1‑100 (MQ=255)

                                                                                       ntGAAACCTTGCGGTAGGCTGATTGTGATGGACGTAATGTCTCCGGGTCACCCAGTGCGCGACATCTGGTTACAGACGGTAGAAGCATTACGCGATACct        >  1:1556165/2‑100 (MQ=255)

                                                                                        tGAAACCTTGCGGTAGGCTGATTGTGGTGGACGTAATGTTTCCGGGTCACCCAGTGCGCGAGATCTGGTTACAGAAGGTAGAAGAATTACGCGATa           >  1:2687536/1‑96 (MQ=255)

                                                                                         gAAACCTTGCGGTAGGCTGATTGTGATGGACGTAATGTCTCCGGGTCACCCAGTGCGCGACATCTGGTTACAGACGGTAGAAGCATTACGCGATACctct      >  2:2480973/1‑100 (MQ=255)

                                                                                         gAAACCTTGCGGTAGGCTGATTGTGATGGACGTAATGTCTCCGGGTCACCCAGTGCGCGACATCTGGTTACAGACGGTAGAAGCATTACGCGATACctct      >  1:758569/1‑100 (MQ=255)

                                                                                         gAAACCTTGCGGTAGGCTGATTGTGATGGACGTAATGTCTCCGGGCCACCCAGTGCGCGACATCTGGTTACAGACGGTAGAAGCATTACGCGATACctct      <  2:659543/100‑1 (MQ=255)

                                                                                             ccTTGCGGTAGGCTGATTGTGATGGACGTAATGTCTCCGGGTCACCCAGTGCGCGACATCTGGTTACAGACGGTAGAAGCATTACGCGATACCTCTCACg  >  2:2236160/1‑100 (MQ=255)

GATAACGCATTTGATATTGTTATCAGCCGTTATTCTGCCCATCACTGGCATGATGTTGGTGCAGCACTGCGAGAAGTGAATAGGATATTGAAACCTGGCGGTAGGCTGATTGTGATGGACGTAATGTCTCCGGGTCACCCAGTGCGCGACATCTGGTTACAGACGGTAGAAGCATTACGCGATACCTCTCACG  >  NC_000913/232100‑232292

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`