Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,600,288 C→T 100% S128L (TCG→TTG)  gcvR → transcriptional repressor, regulatory protein accessory to GcvA

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,600,2880CT98.3% 373.6 / ‑2.4 116S128L (TCG→TTG) gcvRtranscriptional repressor, regulatory protein accessory to GcvA
Reads supporting (aligned to +/- strand):  ref base C (1/1);  new base T (57/57);  total (58/58)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.69e-01

Only 100 of 117 total aligned reads displayed.

GTCTGGGTTCAGGTCGATGTGGCAGACTCCCCGCATTTAATTGAACGCTTCACAGCACTTTTCGACGCGCATCATATGAACATTGCGGAGCTGGTGTCGCGCACGCAACCTGCTGAAAATGAACGGGCTGCGCAGTTGCATATTCAGATAACCGCCCACAGCCCCGCATCTGCGGACGCAGCAAATATTGAGCA  >  NC_000913/2600191‑2600384
                                                                                                 |                                                                                                
gtctgGGTTCAGGTCGATGTGGCAGACTCCCCGCATTTAATTGAACGCTTCACAGCACTTTTCGACGCGCATCATATGAACATTGCGGAGCTGGTGTtgc                                                                                                >  1:1489298/1‑100 (MQ=255)
  ctgGGTTCAGGTCGATGTGGCAGACTCCCCGCATTTAATTGAACGCTTCACAGCACTTTTCGACGCGCATCATATGAACATTGCGGAGCTGGTGTTgcgc                                                                                              <  2:2960451/100‑1 (MQ=255)
   tgtgTTCAGGTTGATGTGGCAGACTCCCCGCATTTAATTGAACGCTTCACAGCACTTTTCGACGCGCATCATATGAACATTGCGGAGCTGGTGTTgcgcn                                                                                             <  2:2211013/97‑2 (MQ=255)
   tgGGTTCAGGTCGATGTGGCAGACTCCCCGCATTTAATTGAACGCTTCACAGCACTTTTCGACGCGCATCATATGAACATTGCGGAGCTGGTGTTGcgca                                                                                             >  2:1301237/1‑100 (MQ=255)
    gggTTCAGGTCGATGTGGCAGACTCCCCGCATTTAATTGAACGCTTCACAGCACTTTTCGACGCGCATCATATGAACATTGCGGAGCTGGTGTTGcgcac                                                                                            >  1:461365/1‑100 (MQ=255)
    gggTTCAGGTCGATGTGGCAGACTCCCCGCATTTAATTGAACGCTTCACAGCACTTTTCGACGCGCATCATATGAACATTGCGGAGCTGGTGTTGcgcac                                                                                            <  2:2352399/100‑1 (MQ=255)
    gggTTCAGGTCGATGTGGCAGACTCCCCGCATTTAATTGAACGCTTCACAGCACTTTTCGACGCGCATCATATGAACATTGCGGAGCTGGTGTTGcgcac                                                                                            >  2:2356461/1‑100 (MQ=255)
     ggTTCAGGTCGATGTGGCAGACTCCCCGCATTTAATTGAACGCTTCACAGCACTTTTCAACGCACATCATATGAACATTACGGAGCTGGTGTTGcgcacg                                                                                           >  1:296884/1‑100 (MQ=255)
           ggTCGATGTGGCAGACTCCCCGCATTTAATTGAACGCTTCACAGCACTTTTCGACGCGCATCATATGAACATTGCGGAGCTGGTGTTGCGCACGCAACct                                                                                     >  1:794626/1‑100 (MQ=255)
           ggTCGATGTGGCAGACTCCCCGCATTTAATTGAACGCTTCACAGCACTTTTCGACGCGCATCATATGAACATTGCGGAGCTGGTGTTGCGCACGCAACct                                                                                     >  2:2115503/1‑100 (MQ=255)
            gTCGATGTGGCAGACTCCCCGCATTTAATTGAACGCTTCACAGCACTTTTCGACGCGCATCATATGAACATTGCGGAGCTGGTGTTGCGCACGCAACctg                                                                                    <  2:2220932/100‑1 (MQ=255)
             tCGATGTGGCAGACTCCCCGCATTTAATTGAACGCTTCACAGCACTTTTCGACGCGCATCATATGAACATTGCGGAGCTGGTGTTGCGCACGCAACctgc                                                                                   <  2:878292/100‑1 (MQ=255)
             tCGATGTGGCAGACTCCCCGCATTTAATTGAACGCTTCACAGCACTTTTCGACGCGCATCATATGAACATTGCGGAGCTGGTGTCGCGCACGCAACctgc                                                                                   >  1:2855309/1‑100 (MQ=255)
             tCGATCTGGCAGACTAAACGAATTTAATTGAAAGCTTCACAGCACTTTTCGACGCGCATCATATGAACATTGCGAAGCTGGTGTTGCGCAAGCAAActg                                                                                    >  2:1491467/1‑99 (MQ=255)
                aTGTGGCAGACTCCCCGCATTTAATTGAACGCTTCACAGCACTTTTCGACGCGCATCATATGAACATTGCGGAGCTGGTGTTGCGTGCGCAAcaccagct                                                                                <  1:819045/100‑8 (MQ=255)
                aTGTGGCAGACTCCCCGCATTTAATTGAACGCTTCACAGCACTTTTCGACGCGCATCATATGAACATTGCGGAGCTGGTGTTGCGCACGCAACCTGCTGa                                                                                >  1:1877933/1‑100 (MQ=255)
                  gtgGCAGACTCCCCGCATTTAATTGAACGCTTCACAGCACTTTTCGACGCGCATCATATGAACATAGCGGAGCTGGTGTTGCGCACGCAACCTGCTGaaa                                                                              >  2:1595020/1‑100 (MQ=255)
                    ggCAGACTCCCCGCATTTAATTGAACGCTTCACAGCACTTTTCGACGCGCATCATATGAACATTGCGGAGCTGGTGTTGCGCACGCAACCTGCTGaaaa                                                                             >  1:2150939/1‑99 (MQ=255)
                    ggCAGACTCCCCGCATTTAATTGAACGCTTCACAGCACTTTTCGACGCGCATCATATGAACATTGCGGAGCTGGTGTTGCGCACGCAACCTGCTGAAAAt                                                                            <  2:600227/100‑1 (MQ=255)
                     gCAGACTCCCCGCATTTAATTGAACGCTTCACAGCACTTTTCGACGCGCATCATATGAACATTGCGGAGCTGGTGTTGCGCACGCAACCTGCTGAAAATg                                                                           <  2:1069254/100‑1 (MQ=255)
                     gCAGACTCCCCGCATTTAATTGAACGCTTCACAGCACTTTTCGACGCGCATCATATGAACATTGCGGAGCTGGTGTTGCGCACGCAACCTGCTGAAAATg                                                                           <  2:1578887/100‑1 (MQ=255)
                     gCAGACTCCCCGCATTTAATTGAACGCTTCACAGCACTTTTCGACGCGCATCATATGAACATTGCGGAGCTGGTGTTGCGCACGCAACCTGCTGAAAATg                                                                           >  1:1770037/1‑100 (MQ=255)
                        gACTCCCCGCATTTAATTGAACGCTTCACAGCACTTTTCGACGCGCATCATATGAACATTGCGGAGCTGGTGTTGCGCACGCAACCTGCTGAAAATGAAc                                                                        <  2:1802555/100‑1 (MQ=255)
                          cTCCCCGCATTTAATTGAACGCTTCACAGCACTTTTCGACGCGCATCATATGAACATTGCGGAGCTGGTGTTGCGCACGCAACCTGCTGAAAATGAACg                                                                       <  1:1974592/99‑1 (MQ=255)
                            ccccGCATTTAATTGAACGCTTCACAGCACTTTTTGACGCGCATCATATGGACATTGCGGAGCTGGTGTTGCGCACGCAACCTGCTGAAAATGAACGGGc                                                                    <  2:1269425/100‑1 (MQ=255)
                            ccccGCATTTAATTGAACGCTTCACAGCACTTTTCGACGCGCATCATATGAACATTGCGGAGCTGGTGTTGCGCACGCAACCTGCTGAAAATGAACGGGc                                                                    >  2:2818303/1‑100 (MQ=255)
                             cccGCATTTAATTGAACGCTTCACAGCACTTTTCGACGCGCATCATATGAACATTGCGGAGCTGGTGTTGCGCACGCAACCTGCTGAAAATGAACGGGCt                                                                   >  1:1065789/1‑100 (MQ=255)
                             cccGCATTTAATTGAACGCTTCACAGCACTTTTCGACGCGCATCATATGAACATTGCGGAGCTGGTGTTGCGCACGCAACCTGCTGAAAATGAACGGGCt                                                                   >  1:163831/1‑100 (MQ=255)
                                gCATTTAATTGAACGCTTCACAGCACTTTTCGACGCGCATCATATGAACATTGCGGAGCTGGTGTTGCGCACGCAACCTGCTGAAAATGAACGGGCTgcg                                                                >  1:531424/1‑100 (MQ=255)
                                gCATTTAATTGAACGCTTCACAGCACTTTTCGACGCGCATCATATGAACATTACGGAGCTGGTGTTGCGCACGCAACCTGCTGAAAATGAACGGGCTgcg                                                                >  1:799544/1‑100 (MQ=255)
                                 cATTTAATTGAACGCTTCACAGCACTTTTCGACGCGCATCATATGTACATTGCGGAGCTGGTGTTGCGCACGCAACCTGCTGAAAATGAACGGGCTgcgc                                                               <  2:2912663/100‑1 (MQ=255)
                                  aTTTAATTGAACGCTTCACAGCACTTTTCGACGCGCATCATATGAACATTGCGGAGCTGGTGTTGCGCACGCAACCTGCTGAAAATGAACGGGCTGCGCa                                                              >  2:1919157/1‑100 (MQ=255)
                                  aTTTAATTGAACGCTTCACAGCACTTTTCGACGCGCATCATATGAACATTGCGGAGCTGGTGTTGCGCACGCAACCTGCTGAAAATGAACGGGCTGCGCa                                                              >  1:2964708/1‑100 (MQ=255)
                                   tttAATTGAACGCTTCACAGCACTTTTCGACGCGCATCATATGAACATTGCGGAGCTGGTGTTGCGCACGCAACCTGCTGAAAATGAACGGGCTGCGCAg                                                             >  1:1926059/1‑100 (MQ=255)
                                    ttAATTGAACGCTTCACAGCACTTTTCGACGCGCATCATATGAACATTGCGGAGCTGGTGTTGCGCACGCAACCTGCTGAAAATGAACGGGCTGCGCAGt                                                            <  2:1315504/100‑1 (MQ=255)
                                     tAATTGAACGCTTCACAGCACTTTTCGACGCGCATCATATGAACATTGCGGAGTTGGTGTTGCGCACGCAACCTGCTGAAAATGAACGGGCTGCGCAGtt                                                           <  1:876539/100‑1 (MQ=255)
                                     tAATTGAACGCTTCACAGCACTTTTCGACGCGCATCATATGAACATTGCGGAGCTGGTGTTGCGCACGCAACCTGCTGAAAATGAACGGGCTGCGCAGtt                                                           >  2:197795/1‑100 (MQ=255)
                                     tAATTGAACGCTTCACAGCACTTTTCGACGCGCATCATATGAACATTGCGGAGCTGGTGTTGCGCACGCAACCTGCTGAAAATGAACGGGCTGCGCAGtt                                                           >  2:1917746/1‑100 (MQ=255)
                                     tAATTGAACGCTTCACAGCACTTTTCGACGCGCATCATATGAACATTGCGGAGCTGGTGTTGCGCACGCAACCTGCTGAAAATGAACGGGCTGCGCAGtt                                                           >  2:264620/1‑100 (MQ=255)
                                      aaTTGAACGCTTCACAGCACTTTTCGACGCGCATCATATGAACATTGCGGAGCTGGTGTTGCGCACGCAACCTGCTGAAAATGAACGGGCTGCGCAGTTg                                                          <  2:5598/100‑1 (MQ=255)
                                      aaTTGAACGCTTCACAGCACTTTTCGACGCGCATCATATGAACATTGCGGAGCTGGTGTCGCGCACGCAACCTGCTGAAAATGAACGGGCTGCGCAGTTg                                                          <  1:2668475/100‑1 (MQ=255)
                                       aTTGAACGCTTCACAGCACTTTTCGACGCGCATCATATGAACATTGCGGAGCTGGTGTTGCGCACGCAACCTGCTGAAAATGAACGGGCTGCGCAGTTGc                                                         >  2:1164381/1‑100 (MQ=255)
                                       aTTGAACGCTTCACAGCACTTTTCGACGCGCATCATATGAACATTGCGGAGCTGGTGTTGCGCACGCAACCTGCTGAAAATGAACGGGCTGCGCAGTTGc                                                         >  2:1578078/1‑100 (MQ=255)
                                        ttGAACGCTTCACAGCACTTTTCGACGCGCATCATATGAACATTGCGGAGCTGGTGTTGCGCACGCAACCTGCTGAAAATGAACGGGCTGCGCAGTTGCa                                                        <  1:420965/100‑1 (MQ=255)
                                        ttGAACGCTTCACAGCACTTTTCGACGCGCATCATATGAACATTGCGGAGCTGGTGTTGCGCACGCAACCTGCTGAAAATGAACGGGCTGCGCAGTTGCa                                                        <  2:961668/100‑1 (MQ=255)
                                        ttGAACGCTTCACAGCACTTTTCGACGCGCATCATATGAACATTGCGGAGCTGGTGTTGCGCACGCAACCTGCTGAAAATGAACGGGCTGCGCAGTTGCa                                                        >  2:1925198/1‑100 (MQ=255)
                                         tGAACGCTTCACAGCACTTTTCGACGCGCATCATATGAACATTGCGGAGCTGGTGTTGCGCACGCAACCTGCTGAAAATGAACGGGCTGCGCAGTTGCat                                                       >  1:746370/1‑100 (MQ=255)
                                         tGAACGCTTCACAGCACTTTTCGACGCGCATCATATGAACATTGCGGAACTGGTGTTGAGCACGCAACCAGCTGAAAATGAACGGGCTGCGCAGATGCa                                                        >  1:196707/1‑99 (MQ=255)
                                          gAACGCTTCACAGCACTTTTCGACGCGCATCATATGAACATTGCGGAGCTGGTGTTGCGCACGCAACCTGCTGAAAATGAACGGGCTGCGCAGTTGCata                                                      >  2:581711/1‑100 (MQ=255)
                                          gAACGCTTCACAGCACTTTTCGACGCGCATCATATGAACATTGCGGAGCTGGTGTTGCGCACGCAACCTGCTGAAAATAAACGAAATGCGCAGTTACata                                                      >  1:1483044/1‑100 (MQ=255)
                                          gAACGCATAACAGCACTTTTCGACGCGCATCATAAGAACATTGCGGAGCTGGTGTTGCGCACGCAACCTGCTGAAAATGAACGGGCTGCGCATTGGCata                                                      >  2:1763059/1‑100 (MQ=255)
                                             cGCTTCACAGCACTTTTCGACGCGCATCATATGAACATTGCGGAGCTGGTGTTGCGCACGCAACCTGCTGAAAATGAACGGGCTGCGCAGTTGCATATTc                                                   <  1:1777107/100‑1 (MQ=255)
                                             cGCTTCACAGCACTTTTCGACGCGCATCATATGAACATTGCGGAGCTGGTGTTGCGCACGCAACCTGCTGAAAATGAACGGGCTGCGCAGTTGCATATTc                                                   <  2:1740943/100‑1 (MQ=255)
                                             cGCTTCACAGCACTTTTCGACGCGCATCATATGAACATTGCGGAGCTGGTGTTGCGCACGCAACCTGCTGAAAATGAACGGGCTGCGCAGTTGCATATTc                                                   >  2:966091/1‑100 (MQ=255)
                                             cGCTTCACAGCACTTTTCGACGCGCATCATATGAACATTGCGGAGATGGTGTTGCGAACACAACATGATGAAAATGAAAGGGCAGGGCAGATGCataatg                                                   >  1:2756170/1‑97 (MQ=255)
                                              gCTTCACAGCACTTTTCGACGCGCATCATATGAACATTGCGGAGCTGGTGTTGCGCACGCAACCTGCTGAAAATGAACGGGCTGCGCAGTTGCATATTCa                                                  <  1:2115526/100‑1 (MQ=255)
                                              gCTTCACAGCACTTTTCGACGCGCATCATATGAACATTGCGGAGCTGGTGTTGCCCACGCAATCTGCTGAAAATGTACGGGCTGTGCAGTTGCATATTCa                                                  <  2:2431869/100‑1 (MQ=255)
                                               cTTCACAGCACTTTTCGACGCGCATCATATGAACATTGCGGAGCTGGTGTTGCGCACGCAACCTGCTGAAAATGAACGGGCTGCGCAGTTGCATATTCa                                                  >  1:899789/1‑99 (MQ=255)
                                                 tCACAGCACTTTTCGACGCGCATCATATGAACATTGCGGAGCTGGTGTTGCGCACGCAACCTGCTGAAAATGAACGGGCTGCGCAGTTGCATATTCAGAt                                               <  1:1318081/100‑1 (MQ=255)
                                                   acaGCACTTTTCGACGCGCATCATATGAACATTGCGGAGCTGGTGTTGCGCACGCAACCTGCTGAAAATGAACGGGCTGCGCAGTTGCATATTCAGATaa                                             >  2:800877/1‑100 (MQ=255)
                                                   acaGCACTTTTCGACGCGCATCATATGAACATTGCGGAGCTGGTGTTGCGCACGCAACCTGCTGAAAATGAACGGGCTGCGCAGTTGCATATTCAGATaa                                             <  1:2570718/100‑1 (MQ=255)
                                                    caGCACTTTTCGACGCGCATCATATGAACATTGCGGAGCTGGTGTTGCGCACGCAACCTGCTGAAAATGAACGGGCTGCGCAGTTGCATATTCAGATAAc                                            >  2:515933/1‑100 (MQ=255)
                                                    caGCACTTTTCGACGCGCATCATATGAACATTGCGGAGCTGGTGTTGCGCACGCAACCTGCTGAAAATGAACGGGCTGCGCAGTTGCATATTCAGATAAc                                            <  1:437525/100‑1 (MQ=255)
                                                     aGCACTTTTCGACGCGCATCATATGAACATTGCGGAGCTGGTGTTGCGCACGCAACCTGCTGAAAATGAACGGGCTGCGCAGTTGCATATTCAGATAAcc                                           <  1:525397/100‑1 (MQ=255)
                                                      gCACTTTTCGACGCGCATCATATGAACATTGCGGAGCTGGTGTTGCGCACGCAACCTGCTGAAAATGAACGGGCTGCGCAGTTGCATATTCAGATAACCg                                          >  2:847997/1‑100 (MQ=255)
                                                      gCACTTTTCGACGCGCATCATATGAACATTGCGGAGCTGGTGTTGCGCACGCAACCTGCTGAAAATGAACGGGCTGCGCAGTTGCATATTCAGATAACCg                                          >  1:2916284/1‑100 (MQ=255)
                                                       cACTTTTCGACGCGCATCATATGAACATTGCGGAGCTGGTGTTGCGCACGCAACCTGCTGAAAATGAACGGGCTGCGCAGTTGCATATTCAGATAACCGc                                         >  2:768341/1‑100 (MQ=255)
                                                       cACTTTTCGACGCGCATCATATGAACATTGCGGAGCTGGTGTTGCGCACGCAACCTGCTGAAAATGAACGGGCTGCGCAGTTGCATATTCAGATAACCGc                                         <  1:1669097/100‑1 (MQ=255)
                                                         cTTTTCGACGCGCATCATATGAACATTGCGGAGCTGGTGTTGCGCACGCAACCTGCTGAAAATGAACGGGCTGCGCAGTTGCATATTCAGATAACCGccc                                       <  2:1845427/100‑1 (MQ=255)
                                                          ttttCGACGCGCATCATATGAACATTGCGGAGCTGGTGTTGCGCACGCAACCTGCTGAAAATGAACGGGCTGCGCAGTTGCATATTCAGATAACCGCCca                                      <  2:2456324/100‑1 (MQ=255)
                                                           tttCGACGCGCATCATATGAACATTGCGGAGCTGGTGTTGCGCACGCAACCTGCTGAAAATGAACGGGCTGCGCAGTTGCATATTCAGATAACCGCCcac                                     <  1:2148272/100‑1 (MQ=255)
                                                              cGACGCGCATCATATGAACATTGCGGAGCTGGTGTTGCGCACGCAACCTGCTGAAAATGAACGGGCTGCGCAGTTGCATATTCAGATAACCGCCCACAGc                                  <  2:899783/100‑1 (MQ=255)
                                                                aCGCGCATCATATGAACATTGCGGAGCTGGTGTTGCGCACGCAACCTGCTGAAAATGAACGGGCTGCGCAGTTGCATATTCAGATAACCGCCCACAGccc                                <  1:1833792/100‑1 (MQ=255)
                                                                 cgcgCATCATATGAACATTGCGGAGCTGGTGTTGCGCACGCAACCTGCTGAAAATGAACGGGCTGCGCAGTTGCATATTCAGATAACCGCCCACAGcccc                               <  2:1489282/100‑1 (MQ=255)
                                                                  gcgcATCATATGAACATTGCGGAGCTGGTGTTGCGCACGCAACCTGCTGAAAATGAACGGGCTGCGCAGTTGCATATTCAGATAACCGCCCACAGCCCCg                              >  1:2963074/1‑100 (MQ=255)
                                                                      atcatATGAACATTGCGGAGCTGGTGTTGCGCACGCAACCTGCTGAAAATGAACGGGCTGCGCAGTTGCATATTCAGATAACCGCCCACAGCCCCGCATc                          >  1:2235655/1‑100 (MQ=255)
                                                                         atatGAACATTGCGGAGCTGGTGTTGCGCACGCAACCTGCTGAAAATGAACGGGCTGCGCAGTTGCATATTCAGATAACCGCCCACAGCCCCGCATCTGc                       <  2:163829/100‑1 (MQ=255)
                                                                          tatGAACATTGCGGAGCTGGTGTTGCGCACGCAACCTGCTGAAAATGAACGGGCTGCGCAGTTGCATATTCAGATAACCGCCCACAGCCCCGCATCTGCg                      <  1:1925359/100‑1 (MQ=255)
                                                                          cttGAACATTGCGGAGCTGGTGTTGCGCACGCAACCTGCTGAAAATGAACGGGCTTCGCAGTTGCATATTCAGATAACCGCCCACAGCCCCGCATCTGCg                      <  2:783165/98‑1 (MQ=255)
                                                                            tGAACATTGCGGAGCTGGTGTTGCGCACGCAACCTGCTGAAAATGAACGGGCAGCGCAGTTGCATATTCAGATAACCGCCCACAACCCCGCATCTGCgag                    >  1:783169/1‑98 (MQ=255)
                                                                             gAACATTGCGGAGCTGGTGTTGCGCACGCAACCTGCTGAAAATGAACGGGCTGCGCAGTTGCATATTCAGATAACCGCCCACAGCCCCGCATCTGCGGAc                   <  1:1428038/100‑1 (MQ=255)
                                                                             gAACATTGCGGAGCTGGTGTTGCGCACGCAACCTGCTGAAAATGAACGGGCTGCGCAGTTGCATATTCAGATAACCGCCCACAGCCCCGCATCTGCGGAc                   <  1:1383903/100‑1 (MQ=255)
                                                                             gAACATTGCGGAGCTGGTGTTGCGCACGCAACCTGCTGAAAATGAACGGGCTGCGCAGTTGCATATTCAGATAACCGCCCACAGCCCCGCATCTGCGGAc                   >  1:2110433/1‑100 (MQ=255)
                                                                                cATTGCGGAGCTGGTGTTGCGCACGCAACCTGCTGAAAATGAACGGGCTGCGCAGTTGCATATTCAGATAACCGCCCACAGCCCCGCATCTGCGGACgca                <  2:2831207/100‑1 (MQ=255)
                                                                                  ttGCGGAGCTGGTGTTGCGCACGCAACCTGCTGAAAATGAACGGGCTGCGCAGTTGCATATTCAGATAACCGCCCACAGCCCCGCATCTGCGGACgcagc              <  1:1763079/100‑1 (MQ=255)
                                                                                  ttGCGGAGCTGGTGTTGCGCACGCAACCTGCTGAAAATGAACGGGCTGCGCAGTTGCATATTCAGATAACCGCCCACAGCCCCGCATCTGCGGACgcagc              <  2:800133/100‑1 (MQ=255)
                                                                                    gCGGAGCTGGTGTTGCGCACGCAACCTGCTGAAAATGAACGGGCTGCGCAGTTGCATATTCAGATAACCGCCCACAGCCCCGCATCTGCGGACGCAGCaa            >  1:2632931/1‑100 (MQ=255)
                                                                                    gCGGAGCTGGTGTTGCGCACGCAACCTGCTGAAAATGAACGGGCTGCGCAGTTGCATATTCAGATAACCGCACACAGCCCCGCATCTGCGGACGCAGCaa            >  1:384818/1‑100 (MQ=255)
                                                                                     cgtaGCTGGTGTTGCGCACGCATCCTGCTGAAAATGAACGGGCTGCGCAGTTGCATATTCAGATAACCGCCCACAGCCCCGCATCTGCGGACGCAGCaaa           <  1:525430/97‑1 (MQ=255)
                                                                                        aGCTGGTGTTGCGCACGCAACGTGCTGAAAATGAACGGGCTGCGCAGTTGCATATTCAGATAACCGCCCACAGCCCCGCATCTGCGGACGCAGCaaa           <  2:2437502/97‑1 (MQ=255)
                                                                                         gCTGGTGTTGCGCACGCAACCTGCTGAAAATGAACGGTCTGCGCAGTTGCATATTCAGATAACCGCCCACAGCCCCGCATCTGCGGACGCAGCAAATAtt       <  1:968338/100‑1 (MQ=255)
                                                                                         gCTGGTGTTGCGCACGCAACCTGCTGAAAATGAACGGGCTGCGCAGTTGCATATTCAGATAACCGCCCACAGCCCCGCATCTGCGGACGCAGCAAATAtt       <  1:2246055/100‑1 (MQ=255)
                                                                                          cTGGTGTTGCGCACGCAACCTGCTGAAAATGAACGGGCTGCGCAGTTGCATATTCAGATAACCGCCCACAGCCCCGCATCTGCGGACGCAGCAAATATTg      <  1:1615220/100‑1 (MQ=255)
                                                                                           tGGTGTTGCGCACGCAACCTGCTGAAAATGAACGGGCTGCGCAGTTGCATATTCAGATAACCGCCCACAGCCCCGCATCTGCGGACGCAGCAAATATTGa     >  2:807348/1‑100 (MQ=255)
                                                                                            ggTGTTGCGCACGCAACCTGCTGAAAATGAACGGGCTGCGCAGTTGCATATTCAGATAACCGCCCACAGCCCCGCATCTGCGGACGCAGCAAATATTGAg    <  2:753879/100‑1 (MQ=255)
                                                                                            ggTGTTGCGCACGCAACCTGCTGAAAATGAACGGGCTGCGCAGTTGCATATTCAGATAACCGCCCACAGCCCCGCATCTGCGGACGCAGCAAATATTGAg    >  1:436484/1‑100 (MQ=255)
                                                                                             gtgtTGCGCACGCAACCTGCTGAAAATGAACGGGCTGCGCAGTTGCATATTCAGATAACCGCCCACAGCCCCGCATCTGCGGACGCAGCAAATATTGAg    <  1:886278/99‑1 (MQ=255)
                                                                                             gtgtTGCGCACGCAACCTGCTGAAAATGAACGGGCTGCGCAGTTGCATATTCAGATAACCGCCCACAGCCCCGCATCTGCGGACGCAGCAAATATTGAGc   <  1:51543/100‑1 (MQ=255)
                                                                                             gtgtTGCGCACGCAACCTGCTGAAAATGAACGGGCTGCGCAGTTGCATATTCAGATAACCGCCCACAGCCCCGCATCTGCGGACGCAGCAAATATTGAGc   <  1:253088/100‑1 (MQ=255)
                                                                                              tgtTGCGCACGCAACCTGCTGAAAATGAACGGGCTGCGCAGTTGCATATTCAGATAACCGCCCACAGCCCCGCATCTGCGGACGCAGCAAATATTGAGCa  >  2:2323545/1‑100 (MQ=255)
                                                                                                 |                                                                                                
GTCTGGGTTCAGGTCGATGTGGCAGACTCCCCGCATTTAATTGAACGCTTCACAGCACTTTTCGACGCGCATCATATGAACATTGCGGAGCTGGTGTCGCGCACGCAACCTGCTGAAAATGAACGGGCTGCGCAGTTGCATATTCAGATAACCGCCCACAGCCCCGCATCTGCGGACGCAGCAAATATTGAGCA  >  NC_000913/2600191‑2600384

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: