Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,707,320 A→T 15.3% intergenic (‑196/+2) lepA ← / ← rseC back‑translocating elongation factor EF4, GTPase/SoxR iron‑sulfur cluster reduction factor component

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,707,3200AT15.3% 324.4 / 43.0 123intergenic (‑196/+2)lepA/rseCback‑translocating elongation factor EF4, GTPase/SoxR iron‑sulfur cluster reduction factor component
Reads supporting (aligned to +/- strand):  ref base A (59/45);  new base T (9/10);  total (68/55)
Fisher's exact test for biased strand distribution p-value = 4.64e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.78e-01

Only 100 of 129 total aligned reads displayed.

CACTACAACATTGAGGCGAGGAAATGTTCATACCGTATGGATTGTGGTATCTGGAAACGTCCTCGCATTTGTTATGCAAAATGCAACAAAGCCAGTGAAATCACTGGCTCGCGTCTTCCGAAGATGTTTCAAATCGCACAAGGCCAGGCGGCAATGCCACGCTTAAGATGATCGGTTGCCATTCTGCCCGGGCAGCAA  >  NC_000913/2707222‑2707419
                                                                                                  |                                                                                                   
cactacaACATTGAGGCGAGGAAATGTTCATACCGTATGGATTGTGGTATCTGGAAACGTCCTCGCATTTGTTATGCAAAATGCAACAAAGCCAGTGaaa                                                                                                    <  2:2840666/100‑1 (MQ=255)
cactacaACATTGAGGCGAGGAAATGTTCATACCGTATGGATTGTGGTATCTGGAAACGTCCTCGCATTTGTTATGCAAAATGCAACAAAGCCAGTGaaa                                                                                                    <  2:1559326/100‑1 (MQ=255)
cactacaACATTGAGGCGAGGAAATGTTCATACCGTATGGATTGTGGTATCTGGAAACGTCCTCGCATTTATTATACAAAATGCAACAAAGCCAATaaaa                                                                                                    >  1:2127313/1‑100 (MQ=255)
  ctacaACATTGAGGCGAGGAAATGTTCATACCGTATGGATTGTGGTATCTGGAAACGTCCTCGCATTTGTTATGCAAAATGCAACAAAGCCAGTGAAATc                                                                                                  >  1:1582948/1‑100 (MQ=255)
  ctacaACATTGAGGCGAGGAAATGTTCATACCGTATGGATTGTGGTATCTGGAAACGTCCTCGCATTTGTTATGCAAAATGCAACAAAGCCAGTGAAATc                                                                                                  >  1:1467836/1‑100 (MQ=255)
     caacaTTGAGGCGAGGAAATGTTCATACCGTATGGATTGTGGTATCTGGAAACGTCCTCGCATTTGTTATGCAAAATGCAACAAAGCCAGTGAAATCACt                                                                                               <  2:369869/100‑1 (MQ=255)
      aacaTTGAGGCGAGGAAATGTTCATACCGTATGGATTGTGGTATCTGGAAACGTCCTCGCATTTGTTATGCAAAATGCAACAAAGCCAGTGAAATCACTg                                                                                              >  2:1950351/1‑100 (MQ=255)
        caTTGAGGCGCGGAAATGTTCATACCGTATGGATTGTGGTATCTGGAAACGTCCTCGCATTTGTTATGCAAAATGCAACAAAGCCAGTGAAATCACTGGc                                                                                            <  2:1986047/100‑1 (MQ=255)
         aTTGAGGCGAGGAAATGTTCATACCGTATGGATTGTGGTATCTGGAAACGTCCTCGCATTTGTTATGCAAAATGCAACAAAGCCAGTGAAATCACTGGc                                                                                            >  1:1657452/1‑99 (MQ=255)
         aTTGAGGCGAGGAAATGTTCATACCGTATGGATTGTGGTATCTGGAAACGTCCTCGCATTTGTTATGCAAAATGCAACAAAGCCAGTGAAATCACTGGCt                                                                                           <  2:2277045/100‑1 (MQ=255)
           tGAGGCGAGGAAATGTTCATACCGTATGGATTGTGGTATCTGGAAACGTCCTCGCATTTGTTATGCAAAATGCAACAAAGCCAGTGATTTCACTGGCttt                                                                                         <  1:2534898/100‑3 (MQ=255)
           tGAGGCGAGGAAATGTTCATACCGTATGGATTGTGGTATCTGGAAACGTCCTCGCATTTGTTATGCAAAATGCAACAAAGCCAGTGATTTCACTGGCttt                                                                                         <  1:1606615/100‑3 (MQ=255)
           tGAGGCGAGGAAATGTTCATACCGTATGGATTGTGGTATCTGGAAACGTCCTCGCATTTGTTATGCAAAATGCAACAAAGCCAGTGATTTCACTGGCt                                                                                           <  2:485545/98‑1 (MQ=255)
             aGGCGAGGAAATGTTCATACCGTATGGATTGTGGTATCTGGAAACGTCCTCGCATTTGTTATGCAAAATGCAACAAAGCCAGTGAAATCACTGGCTcgcg                                                                                       >  2:515451/1‑100 (MQ=255)
               gCGAGGAAATGTTCATACCGTATGGATTGTGGTATCTGGAAACGTCCTCGCATTTGTTATGCAAAATGCAACAAAGCCAGTGATTTCACTGGCtttgttg                                                                                     <  1:1716050/100‑7 (MQ=39)
               gCGAGGAAATGTTCATACCGTATGGATTGTGGTATCTGGAAACGTCCTCGCATTTGTTATGCAAAATGCAACAAAGCCAGTGATTTCACTGGCtttgttg                                                                                     <  1:1821397/100‑7 (MQ=39)
               gCGAGGAAATGTTCATACCGTATGGATTGTGGTATCTGGAAACGTCCTCGCATTTGTTATGCAAAATGCAACAAAGCCAGTGAAATCACTGGCTCGCGTc                                                                                     >  1:801985/1‑100 (MQ=255)
                cGAGGAAATGTTCATACCGTATGGATTGTGGTATCTGGAAACGTCCTCGCATTTGTTATGCAAAATGCAACAAAGCCAGTGATTTCACTGGCtttgttgc                                                                                    <  2:2159244/100‑8 (MQ=38)
                  aGGAAATGTTCATACCGTATGGATTGTGGTATCTGGAAACGTCCTCGCATTTGTTATGCAAAATGCAACAAAGCCAGTGAAATCACTGGCTCGCGTCTTc                                                                                  <  1:2430434/100‑1 (MQ=255)
                   ggAATTGTTCATACCGTATGGATTGTGGTATCTGGAAACGTCCTTGCATTTGTTATGCAAAATGCAACAAAGCCAGTGATTTCACTGGCtttgttg                                                                                     <  1:1808509/96‑7 (MQ=38)
                   ggAAATGTTCATACCGTATGGATTGTGGTATCTTGAAACTTCTTTGTATTTGTTATGCAAAATGCAACAAAGCCAGTGAAATCACTGGCTCGCGTCTTcc                                                                                 <  1:762056/100‑1 (MQ=255)
                   ggAAATGTTCATACCGTATGGATTGTGGTATCTGGAAACGTCCTCGCATTTGTTATGCAAAATGCAACAAAGCCAGTGATTTCACTGGCtttgttgcatt                                                                                 <  1:1049637/100‑11 (MQ=38)
                    gAAATGTTCATACCGTATGGATTGTGGTATCTGGAAACGTCCTCGCATTTGTTATGCAAAATGCAACAAAGCCAGTGAAATCACTGGCTCGCGTCTTCCg                                                                                <  1:138367/100‑1 (MQ=255)
                    gAAATGTTCATACCGGATAGATTGTGGTAACTGGAAACTTCCTCGCATTTGTTATGCAAAATGCAACAAAGCCAGTGAAATCACTGGCTCGCGTCTTcc                                                                                 <  2:234442/99‑1 (MQ=255)
                     aaaTGTTCATACCGTATGGATTGTGGTATCTGGAAACGTCCTCGCATTTGTTATGCAAAATGCAACAAAGCCAGTGAAATCACTGGCTCGCGTCTTCCGa                                                                               >  2:1544240/1‑100 (MQ=255)
                        tGTTCATACCGTATGGATTGTGGTATCTGGAAACGTCCTCGCATTTGTTATGCAAAATGCAACAAAGCCAGTGAAATCACTGGCTCGCGTCTTCCGAAGa                                                                            >  2:2395874/1‑100 (MQ=255)
                         gTTCATACCGTATGGATTGTGGTATCTGGAAACGTCCTCGCATTTGTTATGCAAAATGCAACAAAGCCAGTGAAATCACTGGCTCGCGTCTTCCGa                                                                               >  2:2842472/1‑96 (MQ=255)
                              tACCGTATGGATTGTGGTATCTGGAAACGTCCTCGCATTTGTTATGCAAAATGCAACAAAGCCAGTGAAATCACTGGCTCGCGTCTTCCGAAGATg                                                                          >  2:2461894/1‑96 (MQ=255)
                               aCCGTATGGATTGTGGTATCTGGAAACGTCCTCGCATTTGTTATGCAAAATGCAACAAAGCCAGTGAAATCACTGGCTCGCGTCTTCCGAAGATGTTTCa                                                                     <  1:2761745/100‑1 (MQ=255)
                                  gTATGGATTGTGGTATCTGGAAACGTCCTCGCATTTGTTATGCAAAATGCAACAAAGCCAGTGAAATCACTGGCTCGCGTCTTCCGAAGATGTTTCAAAt                                                                  <  2:1692622/100‑1 (MQ=255)
                                   tATGGATTGTGGTATCTGGAAACGTCCTCGCATTTGTTATGCAAAATGCAACAAAGCCAGTGAATTCACTGGCTCGCGTCTTCCGAAGATGTTTCaaa                                                                   <  2:1693738/98‑1 (MQ=255)
                                   tATGGATTGTGGTATCTGGAAACGTCCTCGCATTTGTTATGCAAAATGCAACAAAGCCAGTGAAATCACTGGCTCGCGTCTTCCGAAGATGTTTCAAATc                                                                 >  2:1376289/1‑100 (MQ=255)
                                   tATGGATTGTGGTATCTGGAAACGTCCTCGCATTTGTTATGCAAAATGCAACAAAGCCAGTGAAATCACTGGCTCGCGTCTTCCGAAGATGTTTCAAATc                                                                 <  1:542415/100‑1 (MQ=255)
                                    aTGGATTGTGGTATCTGGAAACGTCCTCGCATTTGTTATGCAAAATGCAACAAAGCCAGTGAAATCACTGGCTCGCGTCTTCCGAAGATGTTTCAAATCg                                                                <  1:1136399/100‑1 (MQ=255)
                                     tGGATTGTGGTATCTGGAAACGTCCTCGCATTTGTTATGCAAAATGCAACAAAGCCAGTGAAATCACTGGCTCGCGTCTTCCGAAGATGTTTCAAATCGc                                                               >  2:794306/1‑100 (MQ=255)
                                     tGGATTGTGGTATCTGGAAACGTCCTCGCATTTGTTATGCAAAATGCAACAAAGCCAGTGAAATCACTGGCTCGCGTCTTCCGAAGATGTTTCAAATCGc                                                               >  1:666284/1‑100 (MQ=255)
                                     tGGATTGTGGTATCTGGAAACGTCCTCGCATTTGTTATGCAAAATGCAACAAAGCCAGTGAAATCACTGGCTCGCGTCTTCCGAAGATGTTTCAAATCGc                                                               >  1:666293/1‑100 (MQ=255)
                                      ggATTGTGGTATCTGGAAACGTCCTCGCATTTGTTATGCAAAATGCAACAAAACCAGTGAAATCACTAGCTCGCGTCTTCCGAAGATGTTTCAAATCGca                                                              >  1:95230/1‑100 (MQ=255)
                                        aTTGTGGTATCTGGAAACGTCCTCTCATTTGTTATGCAAAATGCAACAAAGCCAGTGAAATCACTGGCTCGCGTCTTCCGAAGATGTTTCAAATCGcaca                                                            <  1:1340353/100‑1 (MQ=255)
                                          tgtgGTATCTGGAAACGTCCTCGCATTTGTTATGCAAAATGCAACAAAGCCAGTGAAATCACTGGCTCGCGTCTTCCGAAGATGTTTCAAATCGCACAAg                                                          <  2:3004053/100‑1 (MQ=255)
                                            tgGTATCGGGAAACGTCCTCGCATTTGTTATGCAAAATGCAACAAAGCCAGTGAAATCACTGGCTCGCGTCTTCCGAAGATGTTTCAAATCGCACAAGGc                                                        <  2:1491226/100‑1 (MQ=255)
                                             ggTATCTGGAAACGTCCTCGCATTTGTTATGCAAAATGCAACAAAGCCAGTGAAATCACTGGCTCGCGTCTTCCGAAGATGTTTCAAATCGCACAAGGcc                                                       >  1:2555274/1‑100 (MQ=255)
                                             ggTATCTGGAAACGTCCTCGCATTTGTTATGCAAAATGCAACAAAGCCAGTGAAATCACTGGCTCGCGTCTTCCGAAGATGTTTCAAATCGCACAAGGcc                                                       >  2:2019834/1‑100 (MQ=255)
                                             ggTATCTGGAAACGTCCTCGCATTTGTTATGCAAAATGCAACAAAGCCAGTGAAATCACTGGCTCGCGTCTTCCGAAGATGTTTCAAATCGCACAAGGcc                                                       >  1:1087928/1‑100 (MQ=255)
                                              gTATCTGGAAACGTCCTCGCATTTGTTATGCAAAATGCAACAAAGCCAGTGAAATCACTGGCTCGCGTCTTCCGAAGATGTTTCAAATCGCACAAGGCCa                                                      >  2:837959/1‑100 (MQ=255)
                                              gTATCTGGAAACGTCCTCGCATTTGTTATGCAAAATGCAACAAAGCCAGTGAAATCACTGGCTCGCGTCTTCCGAAGATGTTTCAAATCGCACAAGGCCa                                                      >  1:2231555/1‑100 (MQ=255)
                                               tATCTGGAAACGTCCTCGCATTTGTTATGCAAAATGCAACAAAGCCAGTGAAATCACTGGCTCGCGTCTTCCGAAGATGTTTCAAATCGCACAAGGCCAg                                                     <  1:2471872/100‑1 (MQ=255)
                                                  cTGGAAACGTCCTCGCATTTGTTATGCAAAATGCAACAACGCCAGTGAAATCACTGGCTCGCGTCTTCCGAAAATGTTTCAAAACGAACAAAGCCAggc                                                   >  1:485792/1‑99 (MQ=255)
                                                   tGGAAACGTCCTCGCATTTGTTATGCAAAATGCAACAAAGCCAGTGAAATCACTGGCTCGCGTCTTCCGAAGATGTTTCAAATCGCACAAGGCCAggcgg                                                 >  2:1387855/1‑100 (MQ=255)
                                                   tGGAAACGTCCTCGCATTTGTTATGCAAAATGCAACAAAGCCAGTGAAATCACTGGCTCGCGTCTTCCGAAGATGTTTCAAATCGCACAAGGCCAggcgg                                                 >  2:2379809/1‑100 (MQ=255)
                                                    ggAAACGTCCTCGCATTTGTTATGCAAAATGCAACAAAGCCAGTGAAATCACTGGCTCGCGTCTTCCGAAGATGTTTCAAATCGCACAAGGCCAggcggc                                                <  1:766492/100‑1 (MQ=255)
                                                    ggAAACGTCCTCGCATTTGTTATGCAAAATGCAACAAAGCCAGTGAAATCACTGGCTCGCGTCTTCCGAAGATGTTTCAAATCGCACAAGGCCAggcggc                                                <  1:1695267/100‑1 (MQ=255)
                                                     gAAACGTCCTCGCATTTGTTATGCAAAATGCAACAAAGCCAGTGAAATCACTGGCTCGCGTCTTCCGAAGATGTTTCAAATCGCACAAGGCCAGGCGGCa                                               >  2:1200095/1‑100 (MQ=255)
                                                     gAAACGTCCTCGCATTTGTTATGCAAAATGCAACAAAACCAGTGAAATCACTGGCTAACGTCTTCCGAAGATGTTTCAAATCGCACAAAGCCAGACGGCa                                               >  2:2994593/1‑100 (MQ=255)
                                                      aaaCGTCCTCGCATTTGTTATGCAAAATGCAACAAAGCCAGTGAAATCACTGGCTCGCGTCTTCCGAAGATGTTTCAAATCGCACAAGGCCAGGCGGCaa                                              <  2:2067298/100‑1 (MQ=255)
                                                         cGTCCTCGCATTTGTTATGCAAAATGCAACAAAGCCAGTGAAATCACTGGCTCGCGCCTTCCGAAGATGATTCAAATCGCACAAGGCCAGGCGGCAATGc                                           >  1:891492/1‑100 (MQ=255)
                                                            ccTCGCATTTGTTATGCAAAATGCAACAAAGCCAGTGAAATCACTGGCTCGCGTCTTCCGAAGATGTTTCAAATCGCACAAGGCCAGGCGGCAATGCCAc                                        >  1:2820748/1‑100 (MQ=255)
                                                            ccTCGCATTTGTTATGCAAAATGCAACAAAGCCAGTGAAATCACTGGCTCGCGTCTTCCGAAGATGTATCAAATCGCACAAGGCCAGGCGGCAATGCCAc                                        >  2:1383755/1‑100 (MQ=255)
                                                             cTCGCATTTGTTATGCAAAATGCAACAAATCCAGTGAAATCACTGGCTCGCGTCTTCCGAAGATGTGTCAAATCGCACAAGGCCAGGCGGCAATGCCACg                                       <  2:1823236/100‑1 (MQ=255)
                                                             cTCGCATTTGTTATGCAAAATGCAACAAAGCCAGTGAAATCACTGGCTCGCGTCTTCCGAAGATGTTTCAAATCGCACAAGGCCAGGCGGCAATGCCACg                                       <  1:122104/100‑1 (MQ=255)
                                                             cTCGCATTTGTTATGCAAAATGCAACAAAGCCAGTGAAATCACTGGCTCGCGTCTTCCGAAGATGTTTCAAATCGCACAAAGCCAGGCGGCAATGCCACg                                       >  1:440025/1‑100 (MQ=255)
                                                              tCGCATTTGTTATGCAAAATGCAACAAAGCCAGTGAAATCACTGGCTCGCGTCTTCCGAAGATGTTTCAAATCGCACAAGGCCAGGCGGCAATGCCACGc                                      >  2:1442530/1‑100 (MQ=255)
                                                               cGCATTTGTTATGCAAAATGCAACAAAGCCAGTGAAATCACTGGCTCGCGTCTTCCGAAGATGTTTCAAATCGCACAAGGCCAGGCGGCAATGCCACGCt                                     >  2:111205/1‑100 (MQ=255)
                                                               cGCATTTGTTATGCAAAATGCAACAAAGCCAGTGAAATCACTGGCTCGCGTCGTCCGAAGAAGTTGCAATTCGCCCAAGGCCAGGCGGCAATGCCACGCt                                     <  1:2712690/100‑1 (MQ=255)
                                                                 cATTTGTTATGCAAAATGCAACAAAGCCAGTGAAATCACTGGCTCGCGTCTTCCGAAGATGTTTCAAATCGCACAAGGCCAGGCGGCAATGCCACGCttn                                   <  2:2906978/100‑2 (MQ=255)
                                                                 cATTTGTTATGCAAAATGCAACAAAGCCAGTGAAATCACTGGCTCGCGTCTTCCGAAGATGTTTCAAATCGCACAAGGCCAGGCGGCAATGCCACGCTTa                                   >  1:1323161/1‑100 (MQ=255)
                                                                  aTTTGTTATGCAAAATGCAACAAAGCCAGTGAAATCACTGGCTCGCGTCTTCCGAAGATGTTTCAAATCGCACAAGGCCAGGCGGCAATGCCACGCTTaa                                  >  1:1006166/1‑100 (MQ=255)
                                                                         aTGCAAAATGCAACAAAGCCAGTGAAATCACTGGCTCGCGTCTTCCGAAGATGTTTCAAATCGCACAAGGCCAGGCGGCAATGCCACGCTTAAGATGATc                           <  2:1088373/100‑1 (MQ=255)
                                                                          tGCAAAATGCAACAAAGCCAGTGAAATCACTGGCTCGCGTCTTCCGAAGATGATTAAAATAGCACAAGGCCAGGCGGCAATGCCACGCTTAAGATGATCg                          >  1:2376455/1‑100 (MQ=255)
                                                                           gCAAAATGCAACAAAGCCAGTGAAATCACTGGCTTGCGTTTTCCGAAGATGTTTCAAATCGCACAAGGCCAGGCGGCAATGCCACGCTTAAGATGATCgg                         <  1:1635721/100‑1 (MQ=255)
                                                                           gCAAAATGCAACAAAGCCAGTGAAATCACTGGCTCGCGTCTTCCGAAGATGTTTCAAATCGCACAAGGCCAGGCGGCAATGCCACGCTTAAGATGATCgg                         >  2:1686939/1‑100 (MQ=255)
                                                                           gCAAAATGCAACAAAGCCAGTGAAATCACTGGCTCGCGTCTTCCGAAGATGTTTCAAATCGCACAAGGCCAGGCGGCAATGCCACGCTTAAGATGATCg                          >  1:3026896/1‑99 (MQ=255)
                                                                           gCAAAATGCAACAAAGCCAGTGAAATCACTGGCTCGCGTCTTCCGAAGATGTTTCAAATCGCACAAGGCCAGGCGGCAATGCCAAGCTTAAgatgat                            >  1:701732/1‑97 (MQ=255)
                                                                            cAAAATGCAACAAAGCCAGTGAAATCACTGGCTCGCGTCTTCCGAAGATGTTTCAAATCGCACAAAGCCAGACGGCAATGCCACGCTTAAGATGATCGGt                        >  1:1326509/1‑100 (MQ=255)
                                                                            cAAAATGCAACAAAGCCAGTGAAATCACTGGCTCGCGTCTTCCGAAGATGTTTCAAATCGCACAAAGACAGGCGGCAATGCCACGCTTAAGATGATCGGt                        >  1:642309/1‑100 (MQ=255)
                                                                              aaaTGCAACAAAGCCAGTGAAATCACTGGCTCGCGTCTTCCGAAGATGTTTCAAATCGCACAAGGCCAGGCGGCAATGCCACGCTTAAGATGATCGGTTg                      <  2:2309370/100‑1 (MQ=255)
                                                                               aaTGCAACAAAGCCAGTGAAATCACTGGCTCGCGTCTTCCGAAGATGTTTCAAATCGCACAAGGCCAGGCGGCAATGCCACGCTTAAGATGATCGGTTGc                     <  1:3036421/100‑1 (MQ=255)
                                                                               aaTGCAACAAAGCCAGTGAAATCACTGGCTCGCGTCTTCCGAAGATGTTTCAAATCGCACAAGGCCAGGCGGCAATGCCACGCTTAAGATGATCGGTTGc                     <  1:2441745/100‑1 (MQ=255)
                                                                                gaagacgcgaGCCAGTGATTTCACTGGCTCGCGTCTTCCGAAGATATTTCAAATCACACAAAGCCAAGCGGCAATGCCACGCTTAAGATGATCGGTTGc                     >  1:1041857/10‑99 (MQ=25)
                                                                                aTGCAACAAAGCCAGTGAAATCACTGGCTCGCGTCTTCCGAAGATGTTTCAAATCGCACAAGGCCAGGCGGCAATGCCACGCTTAAGATGATCGGTTGcc                    <  2:2854429/100‑1 (MQ=255)
                                                                                  gCAACAAAGCCAGTGAAATCACTGGCTCGCGTCTTCCGAAGATGTTTCAAATCGCACAAGGCCAGGCGGCAATGCCACGCTTAAGATGATCGGTTGCCAt                  <  2:2555243/100‑1 (MQ=255)
                                                                                     acaaAGCCAGTGAAATCACTGGCTCGCGTCTTCCGAAGATGTTTCAAATCGCACAAGGCCAGGCGGCAATGCCACGCTTAAGATGATCGGTTGCCATTCt               >  2:569065/1‑100 (MQ=255)
                                                                                      caaAGCCAGTGAAATCACTGGCTCGCGTCTTCCGAAGATGTTTCAAATCGCACAAGGCCAGGCGGCAATGCCACGCTTAAGATGATCGGTTGCCATTCTg              >  1:2781864/1‑100 (MQ=255)
                                                                                       cgaGCCAGTGATTTCACTGGCTCGCGTCTTCCGCAGATGTTTCAAATCGCACAAGGCCAAGCGTCAATGCCACACTTAAAATGATAGGTTGCCATTCTGc             >  1:202383/3‑100 (MQ=255)
                                                                                       cgaGCCAGTGATTTCACTGGCTCGCGTCTTCCGAAGATGTTTCAAATCGCACAAGGCCAGGCGGCAATGCCACGCTTAAGATGATCGGTTGCCATTCTGc             >  1:2374501/3‑100 (MQ=255)
                                                                                       cgaGCCAGTGATTTCACTGGCTCGCGTCTTCCGAAGATGTTTCAAATCGCACAAGGCCAGGCGGCAATGCCACGCTTAAGATGATCGGTTGCCATTCTGc             >  2:117382/3‑100 (MQ=255)
                                                                                       cgaGCCAGTGATTTCACTGGCTCGCGTCTTCCGAAGATGTTTCAAATCGCACAAGGCCAGGCGGCAATGCCACGCTTAAGATGATCGGTTGCCATTCTGc             >  1:2164118/3‑100 (MQ=255)
                                                                                       cgaGCCAGTGATTTCACTGGCTCGCGTCTTCCGAAGATGTTTCAAATCGCACAAGGCCAGACGGCAATGCCACGCTTAAGATGATCGGTTGCCATTCt               >  1:559840/3‑98 (MQ=255)
                                                                                       aaaGCCAGTGAAATCACTGGCTCGCGTCTTCCGAAGATGTTTCAAATCGCACAAGGCCAGGCGGCAATGCCACGCTTAAGATGATCGGTTGCCATTCTGc             <  2:801981/100‑1 (MQ=255)
                                                                                         aGCCAGTGATTTCACTGGCTCGCGTCTTCCGAAGATGTTTCAAATCGCACAAGGCCAGGCGGCAATGCCACGCTTAAGATGATCGGTTGCCATTCTGccc           >  1:2276755/1‑100 (MQ=255)
                                                                                          gCCAGTGATTTCACTGGCTCGCGTCTACCGAAGATGTTTCAAATCGCACAAGGCCAGGCGGCAAAGCCACGCTTAAGATGATCGGCTGCCATTCTGcc            >  2:261344/1‑98 (MQ=255)
                                                                                          gCCAGTGAAATCACTGGCTCGCGTCTTCCGAAGATGTTTCAAATCGCACAAGGCCCGGCGGCAATGCCACGCTTAAGATGATCGGTTGCCATTCTGccc           >  1:2867922/1‑99 (MQ=255)
                                                                                          gCCAGTGAAATCACTGGCTCGCGTCTTCCGAAGATGTTTCAAATCGCACAAGGCCAGGCGGCAATGCCACGCTTAAGATGATCGGTTGCCATTCTGCCCg          >  1:2146148/1‑100 (MQ=255)
                                                                                           ccAGTGAAATCACTGGCTCGCGTCTTCCGAAGATGTTTCAAATCGCACAAGGCCAGGCGGCAATGCCACGCTTAAGATGATCGGTTGCCATTCTGCCCgg         <  2:2155142/100‑1 (MQ=255)
                                                                                             aGTGAAATCACTGGCTCGCGTCTTCCGAAGATGTTTCAAATCGCACAAGGCCAGGCGGCAATGCCACGCTTAAGATGATCGGTTGCCATTCTGCCCgg         >  2:10786/1‑98 (MQ=255)
                                                                                              gTGAAATCACTGGCTCGCGTCTTCCGAAGATGTTTCAAATCGCACAAGGCCAGGCGGCAATGCCACGCTTAAGATGATCGGTTGCCATTCTGCCCGGgca      <  2:208600/100‑1 (MQ=255)
                                                                                                gAAATCACTGTCTCGCGTCTTCCGAAGATGTTTCAAATCGCACAAGGCCAGGCGGCAATGCCACGCTTAAGATGATCGGTTGCCATTCTGCCCGGgcagc    <  2:182681/100‑1 (MQ=255)
                                                                                                gAAATCACTGGCTCGCGTCTTCCGAAGATGTTTCAAATCGCACAAGGCCAGGCGGCAATGCCACGCCTAAGATGATCGGTTGCCATTCTGCCCGGgcagc    >  1:2494746/1‑100 (MQ=255)
                                                                                                 aaaTCACTGGCTCGCGTCTTCCGAAGATGTTTCAAATCGCACAAGGCCAGGCGGCAATGCCACGCTTAAGATGATCGGTTGCCATTCTTCGCGGtcagc    >  2:26472/1‑99 (MQ=255)
                                                                                                  aaTCACTGGCTCGCGTCTTCCGAAGATGTTTCAAATCGCACAAGGCCAGGCTGCAATGCCACGCTTAAGAAGAACGGTTGCCATTCAACCCGGGCAGCaa  >  1:1116611/1‑100 (MQ=255)
                                                                                                  |                                                                                                   
CACTACAACATTGAGGCGAGGAAATGTTCATACCGTATGGATTGTGGTATCTGGAAACGTCCTCGCATTTGTTATGCAAAATGCAACAAAGCCAGTGAAATCACTGGCTCGCGTCTTCCGAAGATGTTTCAAATCGCACAAGGCCAGGCGGCAATGCCACGCTTAAGATGATCGGTTGCCATTCTGCCCGGGCAGCAA  >  NC_000913/2707222‑2707419

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: