Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,900,481 G→T 100% intergenic (‑208/‑111) ygcW ← / → yqcE putative SDR family oxidoreductase/putative MFS transporter, inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,900,4810GT99.3% 437.1 / ‑4.7 136intergenic (‑208/‑111)ygcW/yqcEputative SDR family oxidoreductase/putative MFS transporter, inner membrane protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base T (60/75);  minor base A (1/0);  total (61/75)
Fisher's exact test for biased strand distribution p-value = 4.49e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.34e-01

Only 100 of 137 total aligned reads displayed.

CATAACTGCTAATGTGATCGTAATCACAGTGTGATATTCGTATTGTCAAATAATTTTAAAAAGCAGAAGCGTGATTACTCTCACACAACACCTCTGCTTTCCATGTTATTTATTATTTCAATTACTAGAGACCTGAGAAAAGTAATCCACCTGAAAGGGCGGCTTACTTTTCTCTTTTTTTTGTCTATTACA  >  NC_000913/2900386‑2900577
                                                                                               |                                                                                                
cATAACTGCTAATGTGATCGTAATCACAGTGTGATATTCGTATTGTCAAATAATTTTAAAAAGCAGAAGCGTGATTACTCTCACACAACACCTCTTCttt                                                                                              >  1:800209/1‑100 (MQ=255)
  tAACTGCTAATGTGATTGTAATCACAGTGTGATATTCGTATTGTCAAATAATTTTAAAAAGCAGAAGCGTGATTACTCTCACACAACACCTCTTCTTTcc                                                                                            <  2:2619805/100‑1 (MQ=255)
  tAACTGCTAATGTGATCGTAATCACAGTGTGATATTCGTATTGTCAAATAATTTTAAAAAGCAGAAGCGTGATTACTCTAACACAACACCTCTTCTTTcc                                                                                            <  2:102305/100‑1 (MQ=255)
    aCTGCTAATGTGATCGTAATCACAGTGTGATATTCGTATTGTCAAATAATTTTAAAAAGCAGAAGCGTGATTACTCTCACACAACACCTCTTCTTTCCAt                                                                                          <  1:361404/100‑1 (MQ=255)
    aCTGCTAATGTGATCGTAATCACAGTAGTATATTCGTATTGTCAAATAATTTTAAAAAGCATAAGCGTGATTTCTTTCACACATCACCTCTTCTTTCCAt                                                                                          <  2:475829/100‑1 (MQ=255)
     cTGCTAATGTGATCGTAATCACAGTGTGATATTCGTATTGTCAAATAATTTTAAAAAGCAGAAGCGTGATTACTCTCACACAACACCTCTTCTTTCCAt                                                                                          <  2:2230353/99‑1 (MQ=255)
     cTGCTAATGTGATCGTAATCACAGTGTGATATTCGTATTGTCAAATAATTTTAAAAAGCAGAAGCGTGATTACTCTCACACAACACCTCTTCTTTCCATg                                                                                         <  1:3008217/100‑1 (MQ=255)
       ncTAATGTGATCGTAATCACAGTGTGATATTCGTATTGTCAAATAATTTTAAAAAGCAGAAGCGTGATTACTCTCACACAACACCTCTTCTTTCCATGtt                                                                                       >  1:1298939/2‑100 (MQ=255)
        cTAATGTGATCGTAATCACAGTGTGATATTCGTATTGTCAAATAATTTTAAAAAGCAGAAGCGTGATTACTCTCACACAACACCTCTTCTTTCCATGtta                                                                                      <  2:1510218/100‑1 (MQ=255)
        cTAATGTGATCGTAATCACAGTGTGATATTCGTATTGTCAAATAATTTTAAAAAGCAGAAACGTGATTACTCTCACACAACACATCTTCTTTCCATGtta                                                                                      >  1:1667796/1‑100 (MQ=255)
          aaTGTAATCGTAATCACAGTGTGATATTCGTATTGTCAAATAATTTTAAAAAGCAGAAGCGTGATTACTCTCACACAACACCTCTTCTTTCCATGttatt                                                                                    >  1:2437842/1‑100 (MQ=255)
           aTGTGATCGTAATCACAGTGTGATATTCGTATTGTCAAATAATTTTAAAAAGCAGAAGCGTGATTACTCTCACACAACACCTCTTCTTTCCATGttattt                                                                                   >  2:1136718/1‑100 (MQ=255)
           aTGTGATCGTAATCACAGTGTGATATTCGTATTGTCAAATAATTTTAAAAAGCAGAAGCGTGATTACTCTCACACAACACCTCTTCTTTCCATGttattt                                                                                   >  1:1721009/1‑100 (MQ=255)
             gtgATCGTAATCACAGTGTGATATTCGTATTGTCAAATAATTTTAAAAAGCAGAAGCGTGATTACTCTCACACAACACCTCTTCTTTCCATGttatttat                                                                                 >  2:283148/1‑100 (MQ=255)
             gtgATCGTAATCACAGTGTGATATTCGTATTGTCAAATAATTTTAAAAAGCAGAAGCGTGATTACTCTCACACAACACCTCTTCTTTCCATGttatttat                                                                                 <  2:367804/100‑1 (MQ=255)
               gATCGTAATCACAGTGTGATATTCGTATTGTCAAATAATTTTAAAAAGCAGAAGCGTGATTACTCTCACACAACACCTCTTCTTTCCATGTTATttatta                                                                               >  2:31796/1‑100 (MQ=255)
               gATCGTAATCACAGTGTGATATTCGTATTGTCAAATAATTTTAAAAAGCAGAAGCGTGATTACTCTCACACAACACCTCTTCTTTCCATGTTATttatta                                                                               >  1:2748248/1‑100 (MQ=255)
               gATCGTAATCACAGTGTGATATTCGTATTGTCAAATAATTTTAAAAAGCAGAAGCGTGATTACTCTCACACAACACCTCTACTTTCCATGTTATttatta                                                                               >  1:776255/1‑100 (MQ=255)
                ctCGTAATCACAGTGTGATATTCGTATTGTCAAATAATTTTAAAAAGCAGAAGCGTGATTACTCTCACACAACACCTCTTCTTTCCATGTTATTtattat                                                                              <  1:647394/99‑1 (MQ=255)
                aTCGTAATCACAGTGTGATATTCGTATTGTCAAATAATTTTAAAAAGCAGAAGCGTGATTACTCTCACACAACACCTCTTCTTTCCATGTTATTtattat                                                                              <  2:2501222/100‑1 (MQ=255)
                aTCGTAATCACAGTGTGATATTCGTATTGTCAAATAATTTTAAAAAGCAGAAGCGTGATTACTCTCACACAACACCTCTTCTTTCCATGTTATTtattat                                                                              >  2:1192324/1‑100 (MQ=255)
                 ccgatctCACAGTGTGATATTCGTATTGTCAAATAATTTTAAAAAGCAGAAGCGTGATTACTCTCACACAACACCTCTTCTTTCCATGTTATTTattatt                                                                             <  1:1468812/94‑1 (MQ=255)
                  cGTAATCACAGTGTGATATTCGTATTGTCAAATAATTTTAAAAAGCAGAAGCGTGATTACTCTCACACAACACCTCTTCTTTCCATGTTATTtattatag                                                                            >  2:647390/1‑98 (MQ=255)
                  cGTAATCACAGTGTGATATTCGTATTGTCAAATAATTTTAAAAAGCAGAAGCGTGATTACTCCCACACAACACCTCTTCTTTCCATGTTATTTATTAttt                                                                            <  2:80489/100‑1 (MQ=255)
                   gTAATCACAGTGTGATATTCGTATTGTCAAATAATTTTAAAAAGCAGAAGCGTGATTACTCTCACACAACACCTCTTCTTTCCATGTTATTTATTATTTc                                                                           <  1:2801323/100‑1 (MQ=255)
                    tAATCACAGTGTGATATTCGTATTGTCAAATAATTTTAAAAAGCAGAAGCGTGATTACTCTCACACAACACCTCTTCTTTCCATGTTATTTATTATTTcn                                                                          <  2:1298927/100‑2 (MQ=255)
                    tAATCACAGTGTGATATTCGTATTGTCAAATAATTTTAAAAAGCAGAAGCGTGATTACTCTCACACAACACCTCTTCTTTCCATGTTATTTATTATTTCa                                                                          <  1:1921663/100‑1 (MQ=255)
                     aaTCACAGTGTGATATTCGTATTGTCAAATTATTTTAATTAGCAGAAGCGTGATTACTCTCACACAACACCTCTTCTTTCCATGTTATTTATTATTTCaa                                                                         <  1:184034/100‑1 (MQ=255)
                     aaTCACAGTGTGATATTCGTATTGTCAAATAATTTTAAAAAGCAGAAGCGTGATTACTCTCACACAACACCTCTTCTTTCCATGTTATTTATTATTTCaa                                                                         <  1:3041835/100‑1 (MQ=255)
                      aTCACAGTGTGATATTCGTATTGTCAAATAATTTTAAAAAGCAGAAGCGTGATTACTCTCACACAACACCTCTTCTTTCCATGTTATTTATTATTTCAAt                                                                        >  2:2023834/1‑100 (MQ=255)
                      aTCACAGTGTGATATTCGTATTGTCAAATAATTTTAAAAAGCAGAAGCGTGATTACTCTCACACAACACCTCTTCTTTCCATGTTATTTATTATTTCAAt                                                                        >  1:391163/1‑100 (MQ=255)
                      aTCACAGTGTGATATTCGTATTGTCAAATAATTTTAAAAAGCAGAAGCGTGATTACTCTCACACAACACCTCTTCTTTCCATGTTATTTATTATTTCAAt                                                                        >  2:1053060/1‑100 (MQ=255)
                       tCACAGTGTGATATTCGTATTGTCAAATAATTTTAAAAAGCAGAAGCGTGATTACTCTCACACAACACCTCTTCTTTCCATGTTATTTATTATTTCAAtt                                                                       <  2:2884883/100‑1 (MQ=255)
                       tCACAGTGTGATATTCGTATTGTCAAATAATTTTAAAAAGCAGAAGCGTGATTACTCTCACACAACACCTCTTCTTTCCATGTTATTTATTATTTCAAtt                                                                       <  2:1398905/100‑1 (MQ=255)
                         acaGTGTGATATTCGTATTGTCAAATAATTTTAAAAAGCAGAAGCGTGATTACTCTCACACAATATCTCTTCTTTCCATGTTATTTATTATTTCAATTa                                                                      >  1:1887819/1‑99 (MQ=255)
                         acaGTGTGATATTCGTATTGCCAAATAATTTTAAAAAGCAGAAGCGTGATTACTCTCACACAACACCTCTTCTTTCCATGTTATTTATTATTTCAATTAc                                                                     <  1:2887135/100‑1 (MQ=255)
                          caGTGTGATATTCGTATTGTCAAATAATTTTAAAAAGCAGAAGCGTGATTACTCTCACACAACACCTCTTCTTTCCATGTTATTTATTATTTCAATTACt                                                                    <  2:1338773/100‑1 (MQ=255)
                            gtgtGATATTCGTATTGTCAAATAATTTTAAAAAGCAGAAGCGTGATTACTCTCACACAACACCTCTTCTTTCCATGTTATTTATTGTTTCAATTACtcg                                                                  <  1:2059274/100‑3 (MQ=255)
                            gtgtGATATTCGTATTGTCAAATAATTTTAAAAAGCAGAAGCGTGATTACTCTCACACAACACCTCTTCTTTCCATGTTATTTATTATTTCAATTACTag                                                                  >  2:2468343/1‑100 (MQ=255)
                            gtgtGATATTCGTATTGTCAAATAATTTTAAAAAGCAGAAGCGTGATTACTCTCACACAACACCTCTTCTTTCCATGTTATTTATTATTTCAATTACTag                                                                  <  2:2411481/100‑1 (MQ=255)
                            gtgtGATATTCGTATTGTCAAATAATTTTAAAAAGCAGAAGCGTGATTACTCTCACACAACACCTCTTCTTTCCATGTTATTTATTATTTCAATTACTa                                                                   <  2:1355372/99‑1 (MQ=255)
                               tgATATTCGTATTGTCAAATAATTTTAAAAAGCAGAAGCGTGATTACTCTCACACAACACCTCTTCTTTCCATGTTATTTATTATTTCAATTACTAgaga                                                               >  2:1508444/1‑100 (MQ=255)
                                gATATTCGTATTGTCAAATAATTTTAAAAAGCAGAAGCGTGATTACTCTCACACAACACCTCTTCTTTCCATGTTATTTATTATTTCAATTACTagagtc                                                              >  2:2280773/1‑98 (MQ=255)
                                gATATTCGTATTGTCAAATAATTTTAAAAAGCAGAAGCGTGATTACTCTCACACAACACCTCTTCTTTCCATGTTATTTATTATTTCAATTACTAGAGAc                                                              >  2:707283/1‑100 (MQ=255)
                                  tatTCGTATTGTCAAATAATTTTAAAAAGCAGAAGCGTGATTACTCTCACACAACACCTCTTCTTTCCATGTTATTTATTATTTCAATTACTAGAGACCt                                                            >  2:1120124/1‑100 (MQ=255)
                                  tatTCGTATTGTCAAATAATTTTAAAAAGCAGAAGCGTGATTACTCTCACACAACACCTCTTCTTTCCATGTTATTTATTATTTCAATTACTAGAGACCt                                                            >  1:2413144/1‑100 (MQ=255)
                                   atTCGTATTGTCAAATAATTTTAAAAAGCAAAAGCGTGATTACTCTCACACAACACCTCTTCTTTCCATGTTATTTATTATTTCAATTACTAAAGACCTg                                                           >  2:1757702/1‑100 (MQ=255)
                                    ttCGTATTGTCAAATAATTTTAAAAAGCAGAAGCGTGATTACTCTCACACAACACCTCTTCTTTCCATGTTATTTATTATTTCAATTACTAGAGACCTga                                                          <  1:2110918/100‑1 (MQ=255)
                                       gTATTGTCAAATAATTTTAAAAAGCAGAAGCGTGATTACTCTCACACAACACCTCTTCTTTCCATGTTATTTATTATTTCAATTACTAGAGACCTGAGaa                                                       >  1:508965/1‑100 (MQ=255)
                                        tATTGTCAAATAATTTTTATAAGCAGAAGTGTGATTTCTCTCACACAACACCTCTTCTTTCCATGTTATTTATTATTTCAATTACTAGAGACCTGAGaaa                                                      <  1:1094859/100‑1 (MQ=255)
                                         aTTGTCAAATAATTTTAAAAAGCAGAAGCGTGATTACTCTCACACAACACCTCTTCTTTCCATGTTATTTATTATTTAAATTACTAGAGACTTTAGaaaa                                                     >  1:856142/1‑100 (MQ=255)
                                          ttGTCAAATAATTTTAAAAAGTAGAAGCGTGATTACTCTCACACAACACCTCTTCTTTCCATGTTATTTATTATTTCAATTACTAGAGACCTGAGAAAAg                                                    <  1:1434426/100‑1 (MQ=255)
                                            gTCAAATAATTTTAAAAAGCAGAAGCGTGATTACTCTCACACAACACCTCTTCTTTCCATGTTATTTATTATTTCAATTACTAGAGACCTGAGAAAAGTa                                                  >  1:2644960/1‑100 (MQ=255)
                                              cAAATAATTTTAAAAAGCAGAAGCGTGATTACTCTCACACAACACCTCTTCTTTCCATGTTATTTATTATTTCAATTACTAGAGACCTGAGAAAAGTAAt                                                <  2:837697/100‑1 (MQ=255)
                                                aataatTTTAAAAAGCAGAAGCGTGATTACTCTCACACAACACATCTTCTTTCCATGTTATTTATTATTTCAATTACTAGAGACCTGAGAAAAGTAATcc                                              >  2:1682777/1‑100 (MQ=255)
                                                 ataatTTTAAAAAGCAGAAGCGTGATTACTCTCACACAACACCTCTTCTTTCCATGTTATTTATTATTTCAATTACTAGAGACCTGAGAAAAGTAATCCa                                             <  2:2024610/100‑1 (MQ=255)
                                                  taatTTTAAAAAGCAGAAGCGTGATTACTCTCACACAACACCTCTTCTTTCCATGTTATTTATTATTTCAATTACTAGAGACCTGAGAAAAGTAATCCAc                                            <  1:2941121/100‑1 (MQ=255)
                                                   aatTTTAAAAAGCAGAAGCGTGATTACTCTCACACAACACCTCTTCTTTCCATGTTATTTATTATTTCAATTACTAGAGACCTGAGAAAAGTAATCCa                                             >  1:1736887/1‑98 (MQ=255)
                                                    atTTTAAAAAGCAGAAGCGTGATTACTCTCACACAACACCTCTTCTTTCCATGTTATTTATTATTTCAATTACTAGAGACCTGAGAAAAGTAATCCACCt                                          >  1:1124295/1‑100 (MQ=255)
                                                      tttAAAAAGCAGAAGCGTGATTACTCTCACACAACACCTCTTCTTTCCATGTTATTTATTATTTCAATTACTAGAGACCTGAGAAAAGTAATCCACCTGa                                        >  2:2959477/1‑100 (MQ=255)
                                                         aaaaaGCAGAAGCGTGATTTCTCTCACACAACACCTCTTCTTTCCATGTTATTTATTATTTCAATTACTAGAGACCTGAGAAAAGTAATCCACCTGAAAg                                     <  1:1632090/100‑1 (MQ=255)
                                                          aaaaGCAGAAGCGTGATTACTCTCACACAAAAACTCTTCTTTCCACGTTATTTATTATTTCAATTACTAGAGACCTGAGAAAAGTAATACACCTGAAAg                                     >  2:1974241/1‑99 (MQ=255)
                                                             aGCAGAAGCGTGATTACTCTCACACAACACCTCTTCTTTCCATGTTATTTATTATTTCAATTACTAGAGACCTGAGAAAAGTAATCCACCTGAAAGggcg                                 <  1:2921812/100‑1 (MQ=255)
                                                               cAGAAGCGTGATTACTCTCACACAACACCTCTTCTTTCCATGTTATTTATTATTTCAATTACTAGAGACCTGAGAAAAGTAATCCACCTGAAAGggcggc                               <  1:2280799/100‑1 (MQ=255)
                                                                aGAAGCGTGATTACTCTCACACAACACCTCTTCTTTCCATGTTATTTATTATTTCAATTACTAGAGACCTGAGAAAAGTAATCCACCTGAAAGGGCGGCt                              <  2:1001727/100‑1 (MQ=255)
                                                                  aaGCGTGATTACTCTCACACAACACCTCTTCTTTCCATGTTATTTATTATTTCAATTACTAGAGACCTGAGAAAAGTAATCTACCTGAAAGGGCGGCTTa                            <  2:1063383/100‑1 (MQ=255)
                                                                   aGCGTGATTACTCTCACACAACACCTCTTCTTTCCATGTTATTTATTATTTCAATTACTAGAGACCTGAGAAAAGTAATCCACCTGAAAGGGCGGCTTAc                           <  2:1281096/100‑1 (MQ=255)
                                                                   aGCGTGATTACTCTCACACAACACCTCTTCTTTCCATGTTATTTATTATTTCAATTACTAGAGACCTGAGAAAAGTAATCCACCTGAAAGGGCGGCTTAc                           <  1:968754/100‑1 (MQ=255)
                                                                     cGTGATTTCTCTCACACAACACCTCTTCTTTCCATGTTATTTATTATTTCAATTACTAGAGACCTGAGAAAAGTAATCCACCTGAAAGGGCGGCt                              <  1:1805277/95‑1 (MQ=255)
                                                                      gTGATTACTCTCACACAACACCTCTTCTTTCCATGTTATTTATTATTTCAATTACTAGAGACCTGAGAAAAGTAATCCACCTGAAAGGGCGGCTTACttt                        <  2:802603/100‑1 (MQ=255)
                                                                      gTGATTACTCTCACACAACACCTCTTCTTTCCATGTTATTTATTATTTCAATTACTAGAGACCTGAGAAAAGTAATCCACCTGAAAGGGCGGCTTACttt                        <  2:697027/100‑1 (MQ=255)
                                                                      gTGATTACTCTCACACAACACCTCTTCTTTCCATGTTATTTATTATTTCAATTACTAGAGACCTGAGAAAAGTAATCCACCTGAAAGGGCGGCTTACttt                        >  1:1828076/1‑100 (MQ=255)
                                                                        caTTACTCTCACACAACACCTCTTATTTCCATGTTATTTATTATTACCATTACTACAACCCTGAGAAAAGTAATCAAGCTGAAAGGACAGTTAACtttt                       >  2:684092/2‑99 (MQ=255)
                                                                         aTTACTCTCACACAACACCTCTTCTTTCCATGTTATTTATTATTTCAATTACTAGAGACCTGAGAAAAGTAATCCACCTGAAAGAGCGGCTTACTTTtct                     >  1:1786145/1‑100 (MQ=255)
                                                                          ttACTCTCACACAACACCTCTTCTTTCCATGTTATTTATTATTTCAATTACTAGAGACCTGAGAAAAGTAATCCACCTGAAAGGGCGGCTTACTTTtctc                    <  2:2777866/100‑1 (MQ=255)
                                                                           tACTCTCACACAACACCTCTTCTTTCCATGTTATTTATTATTTCAATTACTAGAGACCTGAGAAAAGTAATCCACCTGAAAGGGCGGCTTACTTTTctct                   >  1:2742929/1‑100 (MQ=255)
                                                                             ctctCACACAACACCTCTTCTTTCCATGTTATTTATTATTTCAATTACTAGAGACCTGAGAAAAGTAATCCACCTGAAAGGGCGGCTTACTTTTCTCttt                 >  1:17275/1‑100 (MQ=255)
                                                                               ctcACACAACACCTCTTCTTTCCATGTTATTTATTATTTCAATTACTAGAGACCTGAGAAAAGTAATCCACCTGAAAGGGCGGCTTACTTTTCTCttttt               >  2:1703442/1‑100 (MQ=255)
                                                                               ctcACACAACACCTCTTCTTTCCATGTTATTTATTATTTCAATTACTAGAGACCTGAGAAAAGTAATCCACCTGAAAGGGCGGCTTACTTTTCTCttttt               >  2:2612598/1‑100 (MQ=255)
                                                                               ctcACACAACACCTCTTCTTTCCATGTTATTTATTATTTCAATTACTAGAGACCTGAGAAAAGTAATCCACCTGAAAGGGCGGCTTACTTTTCTCttttt               >  2:2278653/1‑100 (MQ=255)
                                                                                tcACACAACACCTCTTCTTTCCATGTTATTTATTATTTCAATTACTAGAGACCTGAGAAAAGTAATCCACCTGAAAGGGCGGCTTACTTTTCTCtttttt              <  1:880563/100‑1 (MQ=255)
                                                                                tcACACAACACCTCTTCTTTCCATGTTATTTATTATTTCAATTACTAGAGACCTGAGAAAAGTAATCCACCTGAAAGGGCGGCTTACTTTTCTCtttttt              >  2:2094734/1‑100 (MQ=255)
                                                                                 cacacaACACCTCTTCTTTCCATGTTATTTATTATTTCAATTACTAGAGACCTGAGAAAAGTAATCCACCTGAAAGGGCGGCTTACTTTTCTCttttttt             <  1:1192335/100‑1 (MQ=255)
                                                                                 cacacaACACCTCTTCTTTCCATGTTATTTATTATTTCAATTACTAGAGACCTGAGAAAAGTAATCCACCTGAAAGGGCGGCTTACTTTTCTCttttttt             <  2:800205/100‑1 (MQ=255)
                                                                                 cacacaACACCTCTTCTTTCCATGTTATTTATTATTTCAATTACTAGAGACCTGAGAAAAGTAATCCACCTGAAAGGGCGGCTTACTTTTCTCttttttt             <  1:2390206/100‑1 (MQ=255)
                                                                                 cacacaACACCTCTTCTTTCCATGTTATTTATTATTTCAATTACTAGAGACCTGAGAAAAGTAATCCACCTGAAAGGGCGGCTTACTTTTCTCttttttt             <  2:479044/100‑1 (MQ=255)
                                                                                  acacaACACCTCTTCTTTCCATGTTATTTATTATTTCAATTACTAGAGACCTGAGAAAAGTAATCCACCTGAAAGGGCGGCTTACTTTTCTCtttttttt            <  2:297669/100‑1 (MQ=255)
                                                                                  acacaACACCTCTTCTTTCCATGTTATTTATTATTTCAATTACTAGAGACCTGAGAAAAGTAATCCACCTGAAAGGGCGGCTTACTTTTCTCtttttttt            <  1:1997959/100‑1 (MQ=255)
                                                                                   cacaACACCTCTTCTTTCCATGTTATTTATTATTTCAATTACTAGAGACCTGAGAAAAGTAATCCACCTGAAAGGGCGGCTTACTTTTCTCTTTTTTTTg           <  1:707287/100‑1 (MQ=255)
                                                                                   cacaACACCTCTTCTTTCCATGTTATTTATTATTTCAATTACTAGAGACCTGAGAAAAGTAATCCACCTGAAAGGGCGGCTTACTTTTCTCTTTTTTTTg           >  1:1290656/1‑100 (MQ=255)
                                                                                    acaactCCTCTTCTTTCCATGTTATTTATTATTTCAATTACTAGAGACCTGAGAAAAGTAATCCACCTGAAAGGGCGGCTTACTTTTCTCTTTTTTTTGt          <  1:1360430/100‑1 (MQ=255)
                                                                                     caacaCCTCTTCTTTCCATGTTATTTATTATTTCAATTACTAGAGACCTGAGAAAAGTAATCCACCTGAAAGGGCGGCTTACTTTTCTCTTTTTTTTGTc         <  2:1290644/100‑1 (MQ=255)
                                                                                     caacaCCTCTTCTTTCCATGTTATTTATTATTTCAATTACTAGAGACCTGAGAAAAGTAATCCACCTGAAAGGGCGGCTTACTTTTCTCTTTTTTTTGTc         <  1:1508461/100‑1 (MQ=255)
                                                                                      aacaCCTCTTCTTTCCATGTTATTTATTATTTCAATTACTAGAGACCTGAGAAAAGTAATCCACCTGAAAGGGCGGCTTACTTTTCTCTTTTTTTTGTCt        <  2:2134320/100‑1 (MQ=255)
                                                                                       acacTTCTTTTTTCCATGTTATTTATTATTTCAATTACTAAAAAACTGATAAAATTAATCCACCTGAAAGGGCGTCTTACTTTTCTCTTTTTTTTGTc         <  2:41289/98‑1 (MQ=255)
                                                                                       acacCTCTTCTTTCCATGTTATTTATTATTTCAATTACTAGAGACCTGAGAAAAGTAATCCACCTGAAAGGGCGGCTTACTTTTCTCTTTTTTTTGTCTa       <  2:2838510/100‑1 (MQ=255)
                                                                                          ccTCTTTTTTCCATGTTATTTATTATTTCAATTACTAGAGACCTGAGAAAAGTAATCCACCTGAAAGGGCGGCTTACTTTTCTCttttttttttgt        <  1:1757722/96‑5 (MQ=255)
                                                                                          ccTCTTCTTTCCATGTTATTTATTATTTCAATTACTAGAGTCCTGAGAAAAGTAATCCACCTGAAAGGGCGGCTTACTTTTCTCTTTTTTTTGTCTATTa    <  1:2491490/100‑1 (MQ=255)
                                                                                          ccTCTTCTTTCCATGTTATTTATTATTTCAATTACTAGAGACCTGAGAAAAGTAATCCACCTGAAAGGGCGGCTTACTTTTCTCTTTTTTTTGTCTATTa    >  1:433558/1‑100 (MQ=255)
                                                                                            tcttctTTCCATGTTATTTATTATTTCAATTACTAGAGACCTGAGAAAAGTAATCCACCTGAAAGGGCGTCTTACTTTTCTCTTTTTTTTGTCTTTTACa  >  2:1432389/1‑100 (MQ=255)
                                                                                               |                                                                                                
CATAACTGCTAATGTGATCGTAATCACAGTGTGATATTCGTATTGTCAAATAATTTTAAAAAGCAGAAGCGTGATTACTCTCACACAACACCTCTGCTTTCCATGTTATTTATTATTTCAATTACTAGAGACCTGAGAAAAGTAATCCACCTGAAAGGGCGGCTTACTTTTCTCTTTTTTTTGTCTATTACA  >  NC_000913/2900386‑2900577

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: