Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,971,151 T→C 16.7% intergenic (+18/‑120) ygdQ → / → ygdR UPF0053 family inner membrane protein/DUF903 family verified lipoprotein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,971,1510TC16.7% 272.5 / 47.4 125intergenic (+18/‑120)ygdQ/ygdRUPF0053 family inner membrane protein/DUF903 family verified lipoprotein
Reads supporting (aligned to +/- strand):  ref base T (46/58);  new base C (11/10);  total (57/68)
Fisher's exact test for biased strand distribution p-value = 6.32e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.49e-02

Only 100 of 127 total aligned reads displayed.

GGTTACATCTACTTCGCGATGTTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCGGTTTATAAACAGACTATTATCATAGGT  >  NC_000913/2971053‑2971249
                                                                                                  |                                                                                                  
ggTTACATCTACTTCGCGATGTTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTg                                                                                                   >  1:838280/1‑100 (MQ=255)
  ttACATCTACTTCGCGATGTTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATATTCCGTACGCTCTCCt                                                                                                    >  1:2991079/1‑97 (MQ=255)
  ttACATCTACTTCGCGATGTTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCg                                                                                                 <  1:1868258/100‑1 (MQ=255)
   tACATCTACTTCGCGATGTTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCgg                                                                                                <  1:1358632/100‑1 (MQ=255)
   tACATCTACTTCGCGATGTTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCgg                                                                                                <  2:447325/100‑1 (MQ=255)
   tACATCTACTTCGCGATGTTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCgg                                                                                                >  1:2134201/1‑100 (MQ=255)
       tCTACTTCGCGATGTTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAgg                                                                                            >  2:1179309/1‑100 (MQ=255)
        cTACTTCGCGATGTTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAggg                                                                                           <  1:2901962/100‑1 (MQ=255)
        cTACTTCGCGATGTTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAggg                                                                                           <  1:1887967/100‑1 (MQ=255)
         tACTTCGCGATGTTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAggg                                                                                           >  1:2150793/1‑99 (MQ=255)
             tCGCGATGTTCTTCTCTATTGCGGTTGAAAGCCTCATCTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCCCTCCCGCAGGAGAGCGTac                                                                                      <  1:987018/100‑1 (MQ=38)
             tCGCGATGTTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAATAAAAAGAATCCGCTCTGATACTCCGTACGCCCTCCCGCAGGAGAGCGTac                                                                                      <  1:132635/100‑1 (MQ=38)
             tCGCGATGTTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCCCTCCCGCAGGAGAGCGTac                                                                                      <  1:1060778/100‑1 (MQ=38)
             tCGCGATGTTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCCCTCCCGCAGGAGAGCGTac                                                                                      <  1:2624022/100‑1 (MQ=39)
             tCGCGATGTTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCCCTCCCGCAGGAGAGCGTac                                                                                      <  1:2232384/100‑1 (MQ=38)
             tCGCGATGTTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTAATACTCAGTACGCTCTCCTACGTGAGGGCGTac                                                                                      >  1:2789997/1‑100 (MQ=255)
               gtgATGTTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCCCTCCCGCAGGAGAGCGTacgg                                                                                    <  1:2550492/98‑3 (MQ=38)
               gcgATGTTCTTTTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCCCTCCCGCAGGAGAGCGTacg                                                                                     <  1:683676/99‑2 (MQ=38)
               gcgATGTTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTact                                                                                     >  1:501372/1‑99 (MQ=255)
                cgATGTTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTactca                                                                                   >  2:840038/1‑100 (MQ=255)
                  aTGTTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTactcact                                                                                 <  1:1751585/100‑1 (MQ=255)
                    gTTCTTCTCTATTGCGGTTGTAAGCCTCAACTTGATTCGAAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTcc                                                                               <  1:1770707/100‑1 (MQ=255)
                    gTTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCATACGCTCTCCTGCGGGAGGGCGTACTCACTcc                                                                               <  1:155207/100‑1 (MQ=255)
                     ttcttcTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCt                                                                              >  2:217062/1‑100 (MQ=255)
                     ttcttcTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCCCTCCCGCAGGAGAGCGTacggagtatc                                                                              <  1:2167260/100‑9 (MQ=25)
                       cttctCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCttt                                                                            >  1:2427104/1‑100 (MQ=255)
                         tctcTATTGCGGCTGAAAGACTCAAATTGATTCATAACAAAGAGAATCCGCTCTGAAACTCCGTACGCTCTCCTACGGCAGGCAGTactcact                                                                                 >  2:2448375/1‑93 (MQ=255)
                           tctATTGCGGTTGAAAGCCTCAACTTGATTCGTAACAAATAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTAcc                                                                        <  2:2134178/100‑1 (MQ=255)
                           tctATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGATTCCGCTCTGATACTCCGTACGCTCTTCTGCGGGAGGGCGTACTCACTCCTTTTAcc                                                                        <  2:2116214/100‑1 (MQ=255)
                             tATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCt                                                                      <  2:56308/100‑1 (MQ=255)
                              aTTGCGTTTGAAAGCCTCAACTTAATTCGCAACAAAAGGAAACCGCTCTGATACTCCGTACGCTCTCCTGAGGGAGGGCGTACTCAATCCTTTTACACt                                                                      >  2:1600871/1‑99 (MQ=255)
                              aTTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTc                                                                     <  2:2098330/100‑1 (MQ=255)
                                tGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTcac                                                                   <  2:2143197/100‑1 (MQ=255)
                                 gCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTcaca                                                                  >  1:857321/1‑100 (MQ=255)
                                  cGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAg                                                                 <  1:217064/100‑1 (MQ=255)
                                  cGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAg                                                                 <  1:1698389/100‑1 (MQ=255)
                                    gTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGtt                                                               >  2:1524257/1‑100 (MQ=255)
                                    gTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGtt                                                               >  1:864295/1‑100 (MQ=255)
                                     ttGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGttt                                                              >  1:2274225/1‑100 (MQ=255)
                                     ttGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGttt                                                              <  1:205192/100‑1 (MQ=255)
                                      tGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTa                                                             >  1:1674015/1‑100 (MQ=255)
                                       gAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTaa                                                            <  2:1578227/100‑1 (MQ=255)
                                        aaaGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTACGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAg                                                           >  1:1497069/1‑100 (MQ=255)
                                         aaGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGa                                                          <  2:1578937/100‑1 (MQ=255)
                                          aGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGAt                                                         <  1:2771615/100‑1 (MQ=255)
                                           gCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGAt                                                         <  2:2843476/99‑1 (MQ=255)
                                            ccTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGAttt                                                       >  1:1561108/1‑100 (MQ=255)
                                            ccTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGAttt                                                       >  1:1197207/1‑100 (MQ=255)
                                            ccTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCCCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGAtt                                                        >  1:3017372/1‑99 (MQ=255)
                                             cTCAACTTGATTCGCAACAAAAAGATTCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGAtttt                                                      >  2:783353/1‑100 (MQ=255)
                                              tCAACTTGATTCGTAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTc                                                     >  1:2061505/1‑100 (MQ=255)
                                               cAACTTGATTTGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTcc                                                    <  1:2890871/100‑1 (MQ=255)
                                               cAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTcc                                                    <  2:838276/100‑1 (MQ=255)
                                               cAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTcc                                                    <  2:1757698/100‑1 (MQ=255)
                                                 aCTTGATTCGCAACAAAAAGAACCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTg                                                  >  2:1756014/1‑100 (MQ=255)
                                                      aTTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCGCTGTTTAAGATTTTCCTGCTTTc                                             <  2:384202/100‑1 (MQ=255)
                                                       ttCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCa                                            >  2:941288/1‑100 (MQ=255)
                                                        tCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCaa                                           <  2:2934060/100‑1 (MQ=255)
                                                          gCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGa                                         >  1:2859021/1‑100 (MQ=255)
                                                          gCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGa                                         <  1:1436941/100‑1 (MQ=255)
                                                          gCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGa                                         >  1:1479119/1‑100 (MQ=255)
                                                          gCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGa                                         <  1:1563690/100‑1 (MQ=255)
                                                           caacaaAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGat                                        <  2:1808812/100‑1 (MQ=255)
                                                            aacaacAAGAATCCGCTCTGATACTCCGTACGCTCTCCCGCGGAAGGGCGTACTCACTCCTTTTACCCCCACAGATTAAGATTTTCCTGCTTTCaa                                           >  2:1220203/1‑96 (MQ=255)
                                                            aacaaAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGata                                       >  1:199806/1‑100 (MQ=255)
                                                            aacaaAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGata                                       <  1:1034986/100‑1 (MQ=255)
                                                             acaaAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGatat                                      >  2:182040/1‑100 (MQ=255)
                                                              caaAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGTGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGatata                                     <  2:790456/100‑1 (MQ=255)
                                                               aaaaaGAATCCTCTTTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTATGATTTTCCTGCTTTCAAGatata                                     <  2:351377/99‑1 (MQ=255)
                                                                         cGCTCTGATACTCCGTACGCTCTCCTGCGGGTGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTTCTTTCAAGATATATAACGTCGGtt                          <  2:721719/100‑1 (MQ=255)
                                                                         cGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCGGtt                          <  1:1492103/100‑1 (MQ=255)
                                                                          gCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCGGttt                         <  2:1197196/100‑1 (MQ=255)
                                                                          gCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCGGttt                         <  2:2323385/100‑1 (MQ=255)
                                                                              tGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCGGTTTATaa                     >  2:1391207/1‑100 (MQ=255)
                                                                                ggagtgagTACGCCCTCCCGCAGGAGAGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCGGTTTATAAAc                   >  1:1381939/8‑100 (MQ=37)
                                                                                 gagtgagTACGCCCTCCCGCAGGAGAGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCGGTTTATAAACa                  >  1:270132/7‑100 (MQ=37)
                                                                                  aCTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCGGTTTATAAACAg                 <  2:2406620/100‑1 (MQ=255)
                                                                                   gtgagTACGCCCTCCCGCAGGAGAGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCGGTTTATAAACAGa                >  2:665479/5‑100 (MQ=37)
                                                                                   cTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCGGTTTATAAACAGa                >  2:2175069/1‑100 (MQ=255)
                                                                                   cTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCGGTTTATAAACAGa                <  1:1214472/100‑1 (MQ=255)
                                                                                    tCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCGGTTTATAAACAGAc               >  1:1343112/1‑100 (MQ=255)
                                                                                     gagTACGCCCTCCCGCAGGAGAGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCGGTTTATAAACAGACt              >  1:528030/3‑100 (MQ=38)
                                                                                     ccGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCGGTTTATAAACAGACt              >  1:2248333/1‑100 (MQ=255)
                                                                                     ccGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCGGTTTATAAACAGACt              <  2:2955638/100‑1 (MQ=255)
                                                                                      agTACGCCCTCCCGCAGGAGAGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCGGTTTATAAACAGACta             >  1:1581428/2‑100 (MQ=38)
                                                                                      agTACGCCCTCCCGCAGGAGAGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCGGTTTATAAACAGACta             >  2:2571445/2‑100 (MQ=38)
                                                                                       gTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCGGTTTATAAACAGACtat            <  1:2558790/100‑1 (MQ=255)
                                                                                       gTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCGGTTTATAAACAGACtat            <  1:274223/100‑1 (MQ=255)
                                                                                       gTACGCCCTCCCGCAGGAGAGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCGGTTTATAAACAGACtat            >  2:2106104/1‑100 (MQ=38)
                                                                                       gTACGCCCTCCCGCAGGAGAGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCGGTTTATAAACAGACtat            >  2:1524697/1‑100 (MQ=38)
                                                                                          cgtttgcttGCGGGAGTGCGTACTTACTTCTTTTATCCTCTCAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCGGTTTATAAACAGACtattat         <  1:246797/92‑1 (MQ=255)
                                                                                          cGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCGGTTTATAAACAGACtattat         >  1:246463/1‑100 (MQ=255)
                                                                                          cGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCGGTTTATAAACAGAAtattat         >  1:2288069/1‑100 (MQ=255)
                                                                                            ctctCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCATAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGGCGGTTTATAAACAGACTATTATCa       <  1:1592864/100‑1 (MQ=255)
                                                                                             tctcCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCGGTTTATAAACAGACTATTATCAt      <  2:1575004/100‑1 (MQ=255)
                                                                                              ctcCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCGGTTTATAAACAGACTATTATCAta     >  1:407795/1‑100 (MQ=255)
                                                                                              ctcCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAAATTTTCCTGCTTTCAAGATATATAACGTCGGTTTATAAACAGACTATTATCAta     <  2:1463655/100‑1 (MQ=255)
                                                                                                ccTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCGGTTTATAAACAGACTATTATCAtagg   >  1:238961/1‑100 (MQ=255)
                                                                                                 cTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCGGTTTATAAACAGACTATTATCAtaggt  <  1:525346/100‑1 (MQ=255)
                                                                                                 cTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCGGATTATAAACAGACTATTATCAtag    >  1:2544182/1‑98 (MQ=255)
                                                                                                  |                                                                                                  
GGTTACATCTACTTCGCGATGTTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCGGTTTATAAACAGACTATTATCATAGGT  >  NC_000913/2971053‑2971249

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: