Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,971,154 G→A 15.8% intergenic (+21/‑117) ygdQ → / → ygdR UPF0053 family inner membrane protein/DUF903 family verified lipoprotein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,971,1540GA15.8% 292.4 / 42.2 127intergenic (+21/‑117)ygdQ/ygdRUPF0053 family inner membrane protein/DUF903 family verified lipoprotein
Reads supporting (aligned to +/- strand):  ref base G (47/60);  new base A (10/10);  total (57/70)
Fisher's exact test for biased strand distribution p-value = 6.33e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.15e-02

Only 100 of 132 total aligned reads displayed.

TTACATCTACTTCGCGATGTTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCGGTTTATAAACAGACTATTATCATAGGTAGG  >  NC_000913/2971055‑2971252
                                                                                                   |                                                                                                  
ttACATCTACTTCGCGATGTTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCg                                                                                                    <  1:1868258/100‑1 (MQ=255)
 tACATCTACTTCGCGATGTTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCgg                                                                                                   >  1:2134201/1‑100 (MQ=255)
 tACATCTACTTCGCGATGTTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCgg                                                                                                   <  1:1358632/100‑1 (MQ=255)
    aTCTACTTCGCGATGTTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGa                                                                                                 >  1:2488163/1‑99 (MQ=255)
     tCTACTTCGCGATGTTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAgg                                                                                               >  1:2773998/1‑100 (MQ=255)
      cTACTTCGCGATGTTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAggg                                                                                              <  1:2901962/100‑1 (MQ=255)
      cTACTTCGCGATGTTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAggg                                                                                              <  2:790512/100‑1 (MQ=255)
      cTACTTCGCGATGTTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAggg                                                                                              <  1:1887967/100‑1 (MQ=255)
       tACTTCGCGATGTTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAggg                                                                                              >  1:2150793/1‑99 (MQ=255)
           tCGCGATGTTCTTCTCTATTGCGGTTGAAAGCCTCATCTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCCCTCCCGCAGGAGAGCGTac                                                                                         <  1:987018/100‑1 (MQ=38)
           tCGCGATGTTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAATAAAAAGAATCCGCTCTGATACTCCGTACGCCCTCCCGCAGGAGAGCGTac                                                                                         <  1:132635/100‑1 (MQ=38)
           tCGCGATGTTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCCCTCCCGCAGGAGAGCGTac                                                                                         <  1:1060778/100‑1 (MQ=38)
           tCGCGATGTTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCCCTCCCGCAGGAGAGCGTac                                                                                         <  1:2232384/100‑1 (MQ=38)
           tCGCGATGTTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCCCTCCCGCAGGAGAGCGTac                                                                                         <  1:2624022/100‑1 (MQ=39)
           tCGCGATGTTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTAATACTCAGTACGCTCTCCTACGTGAGGGCGTac                                                                                         >  1:2789997/1‑100 (MQ=255)
             gtgATGTTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCCCTCCCGCAGGAGAGCGTacgg                                                                                       <  1:2550492/98‑3 (MQ=38)
             gcgATGTTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTact                                                                                        >  1:501372/1‑99 (MQ=255)
              cgATGTTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTactca                                                                                      >  2:840038/1‑100 (MQ=255)
                  gTTCTTCTCTATTGCGGTTGTAAGCCTCAACTTGATTCGAAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTcc                                                                                  <  1:1770707/100‑1 (MQ=255)
                  gTTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCATACGCTCTCCTGCGGGAGGGCGTACTCACTcc                                                                                  <  1:155207/100‑1 (MQ=255)
                  gTTCTTCTCTATTGCGCTTGATAGCCTCAACTTGATTCGCAACAAAACGAATCCGCTCTGATACTCCGTACGCCATCCCGCAGGAGAGCGTacc                                                                                        <  2:1107749/94‑2 (MQ=255)
                   ttcttcTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCt                                                                                 >  2:217062/1‑100 (MQ=255)
                   ttcttcTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCCCTCCCGCAGGAGAGCGTacggagtatc                                                                                 <  1:2167260/100‑9 (MQ=25)
                     cttctCTATTGCGGTTGAAAGCCTCATCTTTATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCttt                                                                               <  1:171026/100‑1 (MQ=255)
                     cttctCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCttt                                                                               >  1:2427104/1‑100 (MQ=255)
                       tctcTATTGCGGCTGAAAGACTCAAATTGATTCATAACAAAGAGAATCCGCTCTGAAACTCCGTACGCTCTCCTACGGCAGGCAGTactcact                                                                                    >  2:2448375/1‑93 (MQ=255)
                         tctATTGCGGTTGAAAGCCTCAACTTGATTCGTAACAAATAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTAcc                                                                           <  2:2134178/100‑1 (MQ=255)
                         tctATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGATTCCGCTCTGATACTCCGTACGCTCTTCTGCGGGAGGGCGTACTCACTCCTTTTAcc                                                                           <  2:2116214/100‑1 (MQ=255)
                           tATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCt                                                                         <  2:56308/100‑1 (MQ=255)
                            aTTGCGTTTGAAAGCCTCAACTTAATTCGCAACAAAAGGAAACCGCTCTGATACTCCGTACGCTCTCCTGAGGGAGGGCGTACTCAATCCTTTTACACt                                                                         >  2:1600871/1‑99 (MQ=255)
                             ttGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTca                                                                       <  2:3039792/100‑1 (MQ=255)
                               gCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTcaca                                                                     >  1:857321/1‑100 (MQ=255)
                                cGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAg                                                                    <  1:1698389/100‑1 (MQ=255)
                                cGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAg                                                                    <  1:217064/100‑1 (MQ=255)
                                  gTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGtt                                                                  >  1:864295/1‑100 (MQ=255)
                                  gTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGtt                                                                  >  2:1524257/1‑100 (MQ=255)
                                   ttGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGttt                                                                 >  1:2274225/1‑100 (MQ=255)
                                   ttGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGttt                                                                 <  1:205192/100‑1 (MQ=255)
                                    tGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTa                                                                >  1:1674015/1‑100 (MQ=255)
                                     gAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTaa                                                               >  2:1631803/1‑100 (MQ=255)
                                     gAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTaa                                                               <  2:1578227/100‑1 (MQ=255)
                                      aaaGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAg                                                              <  2:2220257/100‑1 (MQ=255)
                                      aaaGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTACGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAg                                                              >  1:1497069/1‑100 (MQ=255)
                                         gCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGAt                                                            <  2:2843476/99‑1 (MQ=255)
                                         gCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTAAGCTATCCTGCGGGAGAGCGTACTCAATCCTTTTACACTCACAGTTTAAGAt                                                            >  1:2935917/1‑99 (MQ=255)
                                          ccTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGAttt                                                          >  1:1197207/1‑100 (MQ=255)
                                          ccTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCCCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGAtt                                                           >  1:3017372/1‑99 (MQ=255)
                                           cTCAACTTGATTCGCAACAAAAAGATTCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGAtttt                                                         >  2:783353/1‑100 (MQ=255)
                                            tCAACTTGATTCGTAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTc                                                        >  1:2061505/1‑100 (MQ=255)
                                             cAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTcc                                                       <  2:63616/100‑1 (MQ=255)
                                               aCTTGATTCGCAACAAAAAGAACCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTg                                                     >  2:1756014/1‑100 (MQ=255)
                                                    aTTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCGCTGTTTAAGATTTTCCTGCTTTc                                                <  2:384202/100‑1 (MQ=255)
                                                     ttCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCa                                               <  2:2132889/100‑1 (MQ=255)
                                                     ttCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCa                                               >  2:941288/1‑100 (MQ=255)
                                                      tCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCaa                                              <  2:2934060/100‑1 (MQ=255)
                                                        gCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGa                                            >  1:2384457/1‑100 (MQ=255)
                                                        gCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGa                                            <  1:1563690/100‑1 (MQ=255)
                                                        gCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGa                                            >  1:2859021/1‑100 (MQ=255)
                                                        gCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGa                                            >  1:1479119/1‑100 (MQ=255)
                                                        gCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGa                                            <  1:1436941/100‑1 (MQ=255)
                                                         caacaaAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGat                                           <  2:1808812/100‑1 (MQ=255)
                                                          aacaacAAGAATCCGCTCTGATACTCCGTACGCTCTCCCGCGGAAGGGCGTACTCACTCCTTTTACCCCCACAGATTAAGATTTTCCTGCTTTCaa                                              >  2:1220203/1‑96 (MQ=255)
                                                          aacaaAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGata                                          <  1:1034986/100‑1 (MQ=255)
                                                          aacaaAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGata                                          >  1:199806/1‑100 (MQ=255)
                                                           acaaAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGatat                                         >  2:182040/1‑100 (MQ=255)
                                                            caaAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGTGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGatata                                        <  2:790456/100‑1 (MQ=255)
                                                            caaAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGatata                                        <  1:466579/100‑1 (MQ=255)
                                                             aaaaaGAATCCTCTTTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTATGATTTTCCTGCTTTCAAGatata                                        <  2:351377/99‑1 (MQ=255)
                                                                        gCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCGGttt                            <  1:2570879/100‑1 (MQ=255)
                                                                        gCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCGGttt                            <  2:1229081/100‑1 (MQ=255)
                                                                        gCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCGGttt                            <  2:1197196/100‑1 (MQ=255)
                                                                            tGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCTTTCTTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCGGTTtata                         <  1:141861/99‑1 (MQ=255)
                                                                            tGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCGGTTTATaa                        >  2:1391207/1‑100 (MQ=255)
                                                                               gagtgagTACGCCCTCCCGCAGGAGAGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCGGTTTATAAACa                     >  1:270132/7‑100 (MQ=37)
                                                                                aCTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCGGTTTATAAACAg                    <  2:2406620/100‑1 (MQ=255)
                                                                                 gtgagTACGCCCTCCCGCAGGAGAGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCGGTTTATAAACAGa                   >  2:665479/5‑100 (MQ=37)
                                                                                 cTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCGGTTTATAAACAGa                   <  1:1214472/100‑1 (MQ=255)
                                                                                 cTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCGGTTTATAAACAGa                   >  2:2175069/1‑100 (MQ=255)
                                                                                  tgagTACGCCCTCCCGCAGGAGAGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCGGTTTATAAACAGAc                  >  1:1526745/4‑100 (MQ=38)
                                                                                  tCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCGGTTTATAAACAGAc                  >  1:1343112/1‑100 (MQ=255)
                                                                                   gagTACGCCCTCCCGCAGGAGAGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCGGTTTATAAACAGACt                 >  1:528030/3‑100 (MQ=38)
                                                                                   ccGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCGGTTTATAAACAGACt                 >  1:2248333/1‑100 (MQ=255)
                                                                                    agTACGCCCTCCCGCAGGAGAGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCGGTTTATAAACAGACta                >  1:1581428/2‑100 (MQ=38)
                                                                                    agTACGCCCTCCCGCAGGAGAGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCGGTTTATAAACAGACta                >  2:2571445/2‑100 (MQ=38)
                                                                                     gTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCGGTTTATAAACAGACtat               <  1:2558790/100‑1 (MQ=255)
                                                                                     gTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCGGTTTATAAACAGACtat               <  1:274223/100‑1 (MQ=255)
                                                                                     gTACGCCCTCCCGCAGGAGAGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCGGTTTATAAACAGACtat               >  2:2106104/1‑100 (MQ=38)
                                                                                       aCGCCCTCCCGCAGGAGAGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCGGTTTATAAACAGACtatta             >  2:1572542/1‑100 (MQ=255)
                                                                                        cgtttgcttGCGGGAGTGCGTACTTACTTCTTTTATCCTCTCAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCGGTTTATAAACAGACtattat            <  1:246797/92‑1 (MQ=255)
                                                                                          ctctCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCGGTTTATAAACAGACTATTATCa          <  1:1578232/100‑1 (MQ=255)
                                                                                           tctcCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCGGTTTATAAACAGACTATTATCAt         <  2:1575004/100‑1 (MQ=255)
                                                                                            ctcCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCGGTTTATAAACAGACTATTATCAta        <  1:814250/100‑1 (MQ=255)
                                                                                            ctcCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAAATTTTCCTGCTTTCAAGATATATAACGTCGGTTTATAAACAGACTATTATCAta        <  2:1463655/100‑1 (MQ=255)
                                                                                             tcCTGCGGGAGGGCATACTCACTCCTTTTACCCTCACATTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCAGTTTATAAACAAACTATTATc           >  2:1865829/1‑96 (MQ=255)
                                                                                              ccTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCGGTTTATAAACAGACTATTATCAtagg      >  1:238961/1‑100 (MQ=255)
                                                                                               cTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCGGTTTATAAACAGACTATTATCAtaggt     <  1:525346/100‑1 (MQ=255)
                                                                                                  cGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCGGTTTATAAACAGACTATTATCAtaggtagg  <  1:2490590/100‑1 (MQ=255)
                                                                                                  cGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCGGTTTATAAACAGACTATTATCAtaggtagg  <  2:1008418/100‑1 (MQ=255)
                                                                                                  cGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCGGTTTATAAACAGACTATTATCAtaggtagg  <  2:864291/100‑1 (MQ=255)
                                                                                                  cGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCGGTTTATAAACAGACTATTATCAtaggtagg  <  2:2858989/100‑1 (MQ=255)
                                                                                                   |                                                                                                  
TTACATCTACTTCGCGATGTTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCGGTTTATAAACAGACTATTATCATAGGTAGG  >  NC_000913/2971055‑2971252

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: