Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,971,159 G→A 17.0% intergenic (+26/‑112) ygdQ → / → ygdR UPF0053 family inner membrane protein/DUF903 family verified lipoprotein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,971,1590GA17.0% 262.4 / 41.5 122intergenic (+26/‑112)ygdQ/ygdRUPF0053 family inner membrane protein/DUF903 family verified lipoprotein
Reads supporting (aligned to +/- strand):  ref base G (42/58);  new base A (11/10);  total (53/69)
Fisher's exact test for biased strand distribution p-value = 4.70e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.40e-02

Only 100 of 131 total aligned reads displayed.

TCTACTTCGCGATGTTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCGGTTTATAAACAGACTATTATCATAGGTAGGCCAAAT  >  NC_000913/2971060‑2971258
                                                                                                   |                                                                                                   
tCTACTTCGCGATGTTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAgg                                                                                                     >  1:2773998/1‑100 (MQ=255)
 cTACTTCGCGATGTTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAggg                                                                                                    <  2:790512/100‑1 (MQ=255)
 cTACTTCGCGATGTTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAggg                                                                                                    <  1:2901962/100‑1 (MQ=255)
 cTACTTCGCGATGTTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAggg                                                                                                    <  1:1887967/100‑1 (MQ=255)
      tCGCGATGTTCTTCTCTATTGCGGTTGAAAGCCTCATCTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCCCTCCCGCAGGAGAGCGTac                                                                                               <  1:987018/100‑1 (MQ=38)
      tCGCGATGTTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAATAAAAAGAATCCGCTCTGATACTCCGTACGCCCTCCCGCAGGAGAGCGTac                                                                                               <  1:132635/100‑1 (MQ=38)
      tCGCGATGTTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCCCTCCCGCAGGAGAGCGTac                                                                                               <  1:1060778/100‑1 (MQ=38)
      tCGCGATGTTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCCCTCCCGCAGGAGAGCGTac                                                                                               <  1:2624022/100‑1 (MQ=39)
      tCGCGATGTTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCCCTCCCGCAGGAGAGCGTac                                                                                               <  1:2232384/100‑1 (MQ=38)
      tCGCGATGTTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTAATACTCAGTACGCTCTCCTACGTGAGGGCGTac                                                                                               >  1:2789997/1‑100 (MQ=255)
        gtgATGTTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCCCTCCCGCAGGAGAGCGTacgg                                                                                             <  1:2550492/98‑3 (MQ=38)
        gcgATGTTCTTTTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCCCTCCCGCAGGAGAGCGTacg                                                                                              <  1:683676/99‑2 (MQ=38)
        gcgATGTTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTact                                                                                              >  1:501372/1‑99 (MQ=255)
         cgATGTTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTactca                                                                                            >  2:840038/1‑100 (MQ=255)
           aTGTTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTactcact                                                                                          <  1:1751585/100‑1 (MQ=255)
             gTTCTTCTCTATTGCGGTTGTAAGCCTCAACTTGATTCGAAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTcc                                                                                        <  1:1770707/100‑1 (MQ=255)
             gTTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCATACGCTCTCCTGCGGGAGGGCGTACTCACTcc                                                                                        <  1:155207/100‑1 (MQ=255)
             gTTCTTCTCTATTGCGCTTGATAGCCTCAACTTGATTCGCAACAAAACGAATCCGCTCTGATACTCCGTACGCCATCCCGCAGGAGAGCGTacc                                                                                              <  2:1107749/94‑2 (MQ=255)
              ttcttcTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCt                                                                                       >  2:217062/1‑100 (MQ=255)
              ttcttcTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCCCTCCCGCAGGAGAGCGTacggagtatc                                                                                       <  1:2167260/100‑9 (MQ=25)
                cttctCTATTGCGGTTGAAAGCCTCATCTTTATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCttt                                                                                     <  1:171026/100‑1 (MQ=255)
                cttctCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCttt                                                                                     >  1:2427104/1‑100 (MQ=255)
                    tctATTGCGGTTGAAAGCCTCAACTTGATTCGTAACAAATAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTAcc                                                                                 <  2:2134178/100‑1 (MQ=255)
                    tctATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGATTCCGCTCTGATACTCCGTACGCTCTTCTGCGGGAGGGCGTACTCACTCCTTTTAcc                                                                                 <  2:2116214/100‑1 (MQ=255)
                      tATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCt                                                                               <  2:56308/100‑1 (MQ=255)
                       aTTGCGTTTGAAAGCCTCAACTTAATTCGCAACAAAAGGAAACCGCTCTGATACTCCGTACGCTCTCCTGAGGGAGGGCGTACTCAATCCTTTTACACt                                                                               >  2:1600871/1‑99 (MQ=255)
                       aTTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTc                                                                              <  2:2098330/100‑1 (MQ=255)
                        ttGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTca                                                                             <  2:3039792/100‑1 (MQ=255)
                         tGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTcac                                                                            <  2:2143197/100‑1 (MQ=255)
                          gCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTcaca                                                                           >  1:857321/1‑100 (MQ=255)
                           cGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAg                                                                          <  1:217064/100‑1 (MQ=255)
                           cGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAg                                                                          <  1:1698389/100‑1 (MQ=255)
                             gTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGtt                                                                        >  2:1524257/1‑100 (MQ=255)
                              ttGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGttt                                                                       >  1:2274225/1‑100 (MQ=255)
                              ttGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGttt                                                                       <  1:205192/100‑1 (MQ=255)
                                gAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTaa                                                                     <  2:1578227/100‑1 (MQ=255)
                                gAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTaa                                                                     >  2:1631803/1‑100 (MQ=255)
                                 aaaGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAg                                                                    <  2:2684436/100‑1 (MQ=255)
                                 aaaGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAg                                                                    <  2:2220257/100‑1 (MQ=255)
                                 aaaGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTACGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAg                                                                    >  1:1497069/1‑100 (MQ=255)
                                   aGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGAt                                                                  <  1:2771615/100‑1 (MQ=255)
                                    gCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGAt                                                                  <  2:2843476/99‑1 (MQ=255)
                                    gCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTAAGCTATCCTGCGGGAGAGCGTACTCAATCCTTTTACACTCACAGTTTAAGAt                                                                  >  1:2935917/1‑99 (MQ=255)
                                     ccTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGAttt                                                                >  1:1197207/1‑100 (MQ=255)
                                     ccTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCCCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGAtt                                                                 >  1:3017372/1‑99 (MQ=255)
                                      cTCAACTTGATTCGCAACAAAAAGATTCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGAtttt                                                               >  2:783353/1‑100 (MQ=255)
                                       tCAACTTGATTCGTAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTc                                                              >  1:2061505/1‑100 (MQ=255)
                                        cAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTcc                                                             <  2:63616/100‑1 (MQ=255)
                                          aCTTGATTCGCAACAAAAAGAACCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTg                                                           >  2:1756014/1‑100 (MQ=255)
                                           cTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGc                                                          <  1:218417/100‑1 (MQ=255)
                                                ttCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCa                                                     >  2:941288/1‑100 (MQ=255)
                                                   gCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGa                                                  <  1:1563690/100‑1 (MQ=255)
                                                   gCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGa                                                  >  1:2384457/1‑100 (MQ=255)
                                                   gCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGa                                                  >  1:1479119/1‑100 (MQ=255)
                                                   gCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGa                                                  <  1:1436941/100‑1 (MQ=255)
                                                   gCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGa                                                  >  1:2859021/1‑100 (MQ=255)
                                                    caacaaAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGat                                                 <  2:1808812/100‑1 (MQ=255)
                                                     aacaacAAGAATCCGCTCTGATACTCCGTACGCTCTCCCGCGGAAGGGCGTACTCACTCCTTTTACCCCCACAGATTAAGATTTTCCTGCTTTCaa                                                    >  2:1220203/1‑96 (MQ=255)
                                                     aacaaAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGata                                                <  1:1034986/100‑1 (MQ=255)
                                                     aacaaAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGata                                                >  1:199806/1‑100 (MQ=255)
                                                      acaaAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGatat                                               >  2:182040/1‑100 (MQ=255)
                                                       caaAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGTGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGatata                                              <  2:790456/100‑1 (MQ=255)
                                                       caaAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGatata                                              <  1:466579/100‑1 (MQ=255)
                                                        aaaaaGAATCCTCTTTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTATGATTTTCCTGCTTTCAAGatata                                              <  2:351377/99‑1 (MQ=255)
                                                        aaaaaGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGatatat                                             >  1:1266423/1‑100 (MQ=255)
                                                                   gCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCGGttt                                  <  2:1229081/100‑1 (MQ=255)
                                                                   gCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCGGttt                                  <  2:1197196/100‑1 (MQ=255)
                                                                   gCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCGGttt                                  <  2:2323385/100‑1 (MQ=255)
                                                                       tGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCTTTCTTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCGGTTtata                               <  1:141861/99‑1 (MQ=255)
                                                                       tGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCGGTTTATaa                              >  2:1391207/1‑100 (MQ=255)
                                                                          gagtgagTACGCCCTCCCGCAGGAGAGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCGGTTTATAAACa                           >  1:270132/7‑100 (MQ=37)
                                                                           aCTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCGGTTTATAAACAg                          <  2:2406620/100‑1 (MQ=255)
                                                                            gtgagTACGCCCTCCCGCAGGAGAGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCGGTTTATAAACAGa                         >  2:665479/5‑100 (MQ=37)
                                                                             tgagTACGCCCTCCCGCAGGAGAGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCGGTTTATAAACAGAc                        >  1:1526745/4‑100 (MQ=38)
                                                                             tCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCGGTTTATAAACAGAc                        >  1:1343112/1‑100 (MQ=255)
                                                                              ccGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCGGTTTATAAACAGACt                       <  2:2955638/100‑1 (MQ=255)
                                                                               agTACGCCCTCCCGCAGGAGAGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCGGTTTATAAACAGACta                      >  2:2571445/2‑100 (MQ=38)
                                                                               agTACGCCCTCCCGCAGGAGAGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCGGTTTATAAACAGACta                      >  1:1581428/2‑100 (MQ=38)
                                                                                gTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCGGTTTATAAACAGACtat                     <  1:2558790/100‑1 (MQ=255)
                                                                                gTACGCCCTCCCGCAGGAGAGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCGGTTTATAAACAGACtat                     >  2:2106104/1‑100 (MQ=38)
                                                                                  aCGCCCTCCCGCAGGAGAGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCGGTTTATAAACAGACtatta                   >  2:1572542/1‑100 (MQ=255)
                                                                                   cgtttgcttGCGGGAGTGCGTACTTACTTCTTTTATCCTCTCAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCGGTTTATAAACAGACtattat                  <  1:246797/92‑1 (MQ=255)
                                                                                   cGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCGGTTTATAAACAGACtattat                  >  1:246463/1‑100 (MQ=255)
                                                                                   cGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCGGTTTATAAACAGAAtattat                  >  1:2288069/1‑100 (MQ=255)
                                                                                     ctctCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCATAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGGCGGTTTATAAACAGACTATTATCa                <  1:1592864/100‑1 (MQ=255)
                                                                                     ctctCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCGGTTTATAAACAGACTATTATCa                <  1:1578232/100‑1 (MQ=255)
                                                                                      tctcCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCGGTTTATAAACAGACTATTATCAt               <  2:1575004/100‑1 (MQ=255)
                                                                                       ctcCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCGGTTTATAAACAGACTATTATCAta              <  1:814250/100‑1 (MQ=255)
                                                                                       ctcCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAAATTTTCCTGCTTTCAAGATATATAACGTCGGTTTATAAACAGACTATTATCAta              <  2:1463655/100‑1 (MQ=255)
                                                                                        tcCTGCGGGAGGGCATACTCACTCCTTTTACCCTCACATTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCAGTTTATAAACAAACTATTATc                 >  2:1865829/1‑96 (MQ=255)
                                                                                          cTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCGGTTTATAAACAGACTATTATCAtaggt           <  1:525346/100‑1 (MQ=255)
                                                                                          cTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCGGATTATAAACAGACTATTATCAtag             >  1:2544182/1‑98 (MQ=255)
                                                                                            gCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCGGTTTATAAACAGACTATTATCAtaggtag         >  1:2610777/1‑100 (MQ=255)
                                                                                             cGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCGGTTTATAAACAGACTATTATCAtaggtagg        <  2:2858989/100‑1 (MQ=255)
                                                                                             cGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCGGTTTATAAACAGACTATTATCAtaggtagg        <  2:1008418/100‑1 (MQ=255)
                                                                                             cGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCGGTTTATAAACAGACTATTATCAtaggtagg        >  1:867052/1‑100 (MQ=255)
                                                                                             cGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCGGTTTATAAACAGACTATTATCAtaggtagg        <  2:864291/100‑1 (MQ=255)
                                                                                               ggAGGGCGTACTCACTCCTTTTTCCCTCACAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCGGTTTATAAACAGACTATTATCATAGGTAGGcc      <  1:1068859/100‑1 (MQ=255)
                                                                                                   ggCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCGGTTTATAAACAGACTATTATCATAGGTAGGCCAAAt  >  1:1814650/1‑100 (MQ=255)
                                                                                                   ggCGTACTCACTCCTTTTACACTCACAGTTTAAGATTTTCCTGCTTTCAAAATATATAACGTCGGTTTATAAAAAGACTATTATCATAGGTAAGCCAAAt  >  2:1853549/1‑100 (MQ=255)
                                                                                                   |                                                                                                   
TCTACTTCGCGATGTTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCGGTTTATAAACAGACTATTATCATAGGTAGGCCAAAT  >  NC_000913/2971060‑2971258

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: