BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	3,150,120	T→C	100%	F153F (TTT→TTC)	yghA →	putative oxidoreductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,150,120	0	T	C	97.9%	346.6 / ‑2.9	109	F153F (TTT→TTC)	yghA	putative oxidoreductase
Reads supporting (aligned to +/- strand): ref base T (0/3); new base C (48/56); total (49/60)
Fisher's exact test for biased strand distribution p-value = 2.51e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 109 total aligned reads displayed.

AAGCGCACAAGGCGTTAGGCGGGCTGGATATTATGGCGCTGGTCGCCGGGAAACAGGTTGCCATTCCGGATATTGCAGACCTCACCAGCGAACAGTTTCAAAAGACCTTTGCCATTAACGTTTTCGCGCTGTTCTGGCTAACCCAGGAAGCGATCCCCCTGCTACCGAAAGGTGCAAGTATCATCACCACTTCG  >  NC_000913/3150023‑3150216

aaGCGCACAAGGCGTTAGGCGGGCTGGATATTATGGCGCTGGTCGCCGGGAAACAGGTTGCCATTCCGGATATTGCAGACCTCACCAGCGAACAGTTCCa                                                                                                <  2:1104594/100‑1 (MQ=255)

aaGCGCACAAGGCGTTAGGCGGGCTGGATATTATGGCGCTGGTCGCCGGGAAACAGGTTGCCATTCCGGATATTGCAGACCTCACCAGCGAACAGTTCCa                                                                                                <  2:1081813/100‑1 (MQ=255)

    gcACAAGGCGTTAGGCGGGCTGGATATTATGGCGCTGGTCGCCGGGAAACAGGTTGCCATTCCGGATATTGCAGACCTCACCAGCGAACAGTTCCAAAAg                                                                                            >  1:2076611/1‑100 (MQ=255)

     cacaAGGCGTTAGGCGGGCTGGATATTATGGCGCTGGTCGCCGGGAAACAGGTTGCCATTCCGGATATTGCAGACCTCACCAGCGAACAGTTCCAAAAg                                                                                            >  2:908485/1‑99 (MQ=255)

      acaAGTCGTTAGGCGGGCTGGATATTATGGTGCTGGTCGCCGGGAAACAGGTTGCCATTCCGGATATTGCAGACCTCACCAGCGAACAGTTTCAAAAGAc                                                                                          <  2:2801763/100‑1 (MQ=255)

      acaAGGCGTTAGGCGGGCTGGATATTATGGCGCTGGTCGCCGGGAAACAGGTTGCCATTCCGGATATTGCAGACCTCACCAGCGAACAGTTCCAAAAGAc                                                                                          <  2:2357925/100‑1 (MQ=255)

       caAGGCGTTAGGCGGGCTGGATATTATGGCGCTGGTCGCCGGGAAACAGGTTGCCATTCCGGATATTGCAGACCTCACCAGCGAACAGTTCCAAAAGAcc                                                                                         <  2:534559/100‑1 (MQ=255)

       caAGGCGTTAGGCGGGCTGGATATTATGGCGCGGGTCGCCGGGAAACAGGTTGCCATTCCGGATATTGCAGACCTCACCAGCGAACAGTTCCAAAAGAcc                                                                                         >  1:2887733/1‑100 (MQ=255)

         aGGCGTTAGGCGGGCTGGATATTATGGCGCTGGTCGCCGGGAAACAGGTTGCCATTCCGGATATTGCAGACCTCACCAGCGAACAGTTCCAAAAGACCtt                                                                                       >  2:2447947/1‑100 (MQ=255)

          ggCGTTAGGCGGGCTGGATATTATGGCGCTGGTCGCCGGGAAACAGGTTGCCATTCCGGATATTGCAGACCTCACCAGCGAACAGTTCCAAAAGACCttt                                                                                      >  2:2470302/1‑100 (MQ=255)

            cGTTAGGCGGGCTGGATATTATGGCGCTGGTCGCCGGGAAACAGGTTGCCATTCCGGATATTGCAGACCTCACCAGCGAACAGTTCCAAGAGACCTTTGc                                                                                    >  1:1725549/1‑100 (MQ=255)

            cGTTAGGCGGGCTGGATATTATGGCGCTGGTCGCCGGGAAACAGGTTGCCATTCCGGATATTGCAGACCTCACCAGCGAACAGTTCCAAAAGACCTTTg                                                                                     >  1:480484/1‑99 (MQ=255)

             gTTAGGCGGGCTGGATATTATGGCGCTGGTCGCCGGGAAACAGGTTGCCATTCCGGATATTGCAGATCTCACCAGCGAACAGTTCCAAAAGACCTTTGcc                                                                                   <  1:1164968/100‑1 (MQ=255)

              ttAGGCGGGCTGGATATTATGGCGCTGGTCGCCGGGAAACAGGTTGCCATTCCGGATATTGCAGACCTCACCAGCGAACAGTTCCAAAAGACCTTTGCCa                                                                                  <  1:2645459/100‑1 (MQ=255)

               tAGGCGGGCTGGATATTATGGCGCTGGTCGCCGGGAAACAGGTTGCCATTCCGGATATTGCAGACCTCACCAGCGAACAGTTCCAAAAGACCTTTGCCAt                                                                                 <  2:1599007/100‑1 (MQ=255)

                 ggCGGGCTGGATATTATGGCGCTGGTCGCCGGGAAACAGGTTGCCATTCCGGATATTGCAGACCTCACCAGCGAACAGTTCCAAAAGACCTTTGCCATTa                                                                               >  2:1519678/1‑100 (MQ=255)

                        tGGATATTATGGCGCTGGTCGCCGGGAAACAGGTTGCCATTCCGGATATTGCAGACCTCACCAGCGAACAGTTCCAAAAGACCTTTGCCATTAACGtttt                                                                        <  2:724489/100‑1 (MQ=255)

                         ggATATTATGGCGCTGGTCGCCGGGAAACAGGTTGCCATTCCGGATATTGCAGACCTCACCAGCGAACAGTTCCAAAAGACCTTTGCCATTAACGTTTTc                                                                       >  2:652645/1‑100 (MQ=255)

                          gATATTATGGCGCTGGTCGCCGGGAAACAGGTTGCCATTCCGGATATTGCAGACCTCACCAGCGAACAGTTCCAAAAGACCTTTGCCATTAACGTTTTcg                                                                      >  2:2058008/1‑100 (MQ=255)

                          gATATTATGGCGCTGGTCGCCGGGAAACAGGTTGCCATTCCGGATATTGCAGACCTCACCAGCGAACAGTTCCAAAAGACCTTTGCCATTAACGTTTTcg                                                                      <  1:2628618/100‑1 (MQ=255)

                           atatTATGGCGCTGGTCGCCGGGAAACAGGTTGCCATTCCGGATATTGCAGACCTCACCAGCGAACAGTTCCAAAAGACCTTTGCCATTAACGTTTTcn                                                                      <  2:2514758/99‑2 (MQ=255)

                             attatGGCGCTGGTCGCCGGGAAACAGGTTGCCATTCCGTATATTGCAGACCTCACCAGCGAACAGTTCCAAAAGACCTTTGCCATTAACGTTTTCgcgc                                                                   <  2:2750494/100‑1 (MQ=255)

                               tatGGCGCTGGTCGCCGGGAAACAGGTTGCCATTCCGGATATTGCAGACCTCACCAGCGAACAGTTCCAAAAGACCTTTGCCATAAACGTTTTCGCGCTg                                                                 >  1:1386810/1‑100 (MQ=255)

                                atGGCGCTGGTCGCCGGGAAACAGGTTGCCATTCCGGATATTGCAGACCTCACCAGCGAACAGTTCCAAAAGACCTTTGCCATTAACGTTTTCGCGCTGt                                                                <  2:521733/100‑1 (MQ=255)

                                      cTGGTCGCCGGGAAACAGGTTGTCATTCCGGATATTGCAGACCTCACCAGCGAACAGTTCCAAAAGACCTTTGCCATTAACGTTTTCGCGCTGTTCTGGc                                                          <  1:156553/100‑1 (MQ=255)

                                      cTGGTCGCCGGGAAACAGGTTGCCATTCCGGATATTGCAGACCTCACCAGCGAACAGTTCCAAAAGACCTTTGCCATTAACGTTTTCGCGCTGTTCTGGc                                                          <  1:318524/100‑1 (MQ=255)

                                      cTGGTCGCCGGGAAACAGGTTGCCATTCCGGATATTGCAGACCTCACCAGCGAACAGTTCCAAAAGACCTTTGCCATTAACGTTTTCGCGCTGTTCTGGc                                                          <  2:1653641/100‑1 (MQ=255)

                                       tGGTCGCCGGGAAACAGGTTGCCATTCCGGATATTGCAGACCTCACCAGCGAACAGTTCCAAAAGACCTTTGCCATTAACGTTTTCGCGCTGTTCTGGCt                                                         <  1:632044/100‑1 (MQ=255)

                                         gTCGCCGGGAAACAGGTTGCCATTCCGGATATTGCAGACCTCACCAGCGAACAGTTCTAAAAGACCTTTGCCATTAACGTTTTCGCGCTGTTCTGGCTa                                                        <  1:2536035/99‑1 (MQ=255)

                                           cGCCGTGAAACAGCTTGCCATTCCGGAAATTGCAGAACTCACCAGCGAACAGTTCCAAAAGACCTTTGCCAACAACGTTTTCGCGCTGTTCTGGCCAAcc                                                     >  2:1624095/1‑100 (MQ=255)

                                           cGCCGGGAAACAGGTTGCCATTCCGGATATTGCAGACCTCACCAGCGAACAGTTCCAAAAGACCTTTGCCATTAACGTTTTCGCGCTGTTCTGGCTAAcc                                                     >  2:2231777/1‑100 (MQ=255)

                                           cGCCGGGAAACAGGTTGCCATTCCGGATATTGCAGACCTCACCAGCGAACAGTTCCAAAAGACCTTTGCCATTAACGTTTTAGCGCTGTTCTGGATa                                                        >  2:2742857/1‑97 (MQ=255)

                                           cGCCGGGAAACAGGTTGCCATTCCGGATATTGCAGACCTCACCAGCGAACAGTTCCAAAAAACCTTTGCCATTAACGTTTTCGCGCTGTTCTGGCTAAcc                                                     >  1:1852781/1‑100 (MQ=255)

                                           cGCCGGGAAACAGGTTGCCATTCCGGATATTGCAGACCTCACCAGCGAACAGTTACAAAAGACCTTTGACATTAACGTTTTCGCGCTGTTCTGGCTaa                                                       >  1:2413055/1‑98 (MQ=255)

                                            gCCGGGAAACAGGTTGCCATTCCGGATATTGCAGTCTTCACCAGCGAACAGTTTCAAAAGACCTTTGCCATTAACGTTTTCGCGCTGTTCTGGCTAAccc                                                    <  1:1825964/100‑1 (MQ=255)

                                             ccGGGAAACAGGTTGCCATTCCGGATATTGCAGACCTCACCAGCGAACAGTTCCAAAAGACCTTTGCCATTAACGTTTTCGCGCTGTTCTGGCTAACCCa                                                   <  2:129808/100‑1 (MQ=255)

                                               gggAAACAGGTTGCCATTCCGGATATTGCAGACCTCACCAGCGAACAGTTCCAAAAGACCTTTGCCATTAACGTTTTCGCGCTGTTCTGGCTAACCCAgg                                                 >  1:2251311/1‑100 (MQ=255)

                                                ggAAACAGGTTGCCATTCCGGATATTGCAGACCTCACCAGCGAACAGTTCCAAAAGACCTTTGCCATTAACGTTTTCGCGCTGTTCTGGCTAACCCAGGa                                                <  2:652889/100‑1 (MQ=255)

                                                ggAAACAGGTTGCCATTCCGGATATTGCAGACCTCACCAGCGAACAGTTCCAAAAGACCTTTGCCATTAACGTTTTCGCGCTGTTCTGGCTAACCCAGGa                                                >  2:1317762/1‑100 (MQ=255)

                                                 gAAACAGGTTGCCATTCCGGATATTGCAGACCTCACCAGCGAACAGTTCCAAAAGACCTTTGCCATTAACGTTTTCGCGCTGTTCTGGCTAACCCAGGaa                                               >  1:2612492/1‑100 (MQ=255)

                                                  aaaCAGGTTGCCATTCCTGATATTGCAGACCTCACCAGCGAACAGTTTCAAAAGACCTTTGCCATTAACGTTTTCGCGCTGTTCTGGCTAACCCAGGa                                                <  1:2226110/98‑1 (MQ=255)

                                                   aaCAGGTTGCCATTCCGGATATTGCAGACCTCACCAGCGAACAGTTCCAAAAGACCTTTGCCATTAACGTTTTCGCGCTGTTCTGGCTAACCCAGGAAGc                                             >  2:1297232/1‑100 (MQ=255)

                                                   aaCAGGTTGCCATTCCGGATATTGCAGACCTCACCAGCGAACAGTTCCAAAAGACCTTTGCCATTAACGTTTTCGCGCTGTTCTGGCTAACCCAGGAAGc                                             <  1:2447976/100‑1 (MQ=255)

                                                   aaCAGGTTGCCATTCCGGATATTGCAGACCTCACCAGCGAACAGTTCCAAAAGACCTTTGCCATTAACGTTTTCGCGCTGTTCTGGCTAACCCAGGAAGc                                             >  2:533028/1‑100 (MQ=255)

                                                    aCAGGTTGCCATTCCGGATATTGCAGACCGCACCAGCGAACAGTTCCAAAAGACCTTTGCCATTAACGTTTTCGCGCTGTTCTGGCTAACCCAGGAAGc                                             <  2:344588/99‑1 (MQ=255)

                                                     cAGGTTGCCATTCCGGATATTGCAGACCTCACCAGCGAACAGTTCCAAAAGACCTTTGCCATTAACGTTTTCGCGCTGTTCTGGCTAACCCAGGAAGCGa                                           <  1:1541668/100‑1 (MQ=255)

                                                       ggTTGCCATTCCGGATATTGCAGACCTCACCAGCGAACAGTTCCAAAAGACCTTTGCCATTAACGTTTTCGCGCTGTTCTGGCTAACCCAGAAAGCGAt                                          >  1:732927/1‑99 (MQ=255)

                                                        gTTGCCATTCCGGATATTGCAGACCTCACCAGCGAACAGTTCCAAAAGACCTTTGCCATTAACGTTTTCGCGCTGTTCTGGCTAACCCAGGAAGCGATcc                                        <  2:1242211/100‑1 (MQ=255)

                                                         ttGCCATTCCGGATATTGCAGACCTCACCAGCGAACAGTTCCAAAAGACCTTTGCCATTAACGTTTTCGCGCTGTTCTGGCTAACCCAGGAAGCGATccc                                       <  1:263987/100‑1 (MQ=255)

                                                           gCCATTCCGGATATTGCAGACCTCACCAGCGAACAGTTCCAAAAGATCTTTGCCATTAACGTTTTCGCGCTGTTCTGGCTAACCCAGGAAGCGATccccc                                     <  1:1041140/100‑1 (MQ=255)

                                                           gCCATTCCGGATATTGCAGACCTCACCAGCGAACAGTTCCAAAAGACCTTTGCCATTAACGTTTTCGCGCTGTTCTGGCTAACCCAGGAAGCGATccccc                                     >  1:1056221/1‑100 (MQ=255)

                                                           gCCATTCCGGATATTGCAGACCTCACCAGCGAACAGTTCCAAAAGACCTTTGCCATTAACGTTTTCGCGCTGTTCTGGCTAACCCAGGAAGCGATccccc                                     <  1:1065358/100‑1 (MQ=255)

                                                           gCCATTCCGGATATTGCAGACCTCACCAGCGAACAGTTCCAAAAGACCTTTGCCATTAACGTTTTCGCGCTGTTCTGGCTAACCCAGGAAGCGATccccc                                     >  1:118976/1‑100 (MQ=255)

                                                             cATTCCGGATATTGCAGACCTCACCAGCGAACAGTTCCAAAAGACCTTTACCATTAACGTTTTCGCGCTGTTCTGGCTAACCCAGGAAGCGATCCCCCTg                                   <  1:102711/100‑1 (MQ=255)

                                                                tCCGGATATTGCAGACCTCACCAGCGAACAGTTCCAAAAGACCTTTGCCATTAACGTTTTCGCGCTGTTCTGGCTAACCCAGGAAGCGATCCCCCTGCTa                                >  2:134271/1‑100 (MQ=255)

                                                                tCCGGATATTGCAGACCTCACCAGCGAACAGTTCCAAAAGACCTTTGCCATTAACGTTTTCGCGCTGTTCTGGCTAACCCAGGAAGCGATCCCCCTGCTa                                <  2:2806266/100‑1 (MQ=255)

                                                                tCCGGATATTGCAGACCTCACCAGCGAACAGTTCCAAAAGACCTTTGCCATTAACGTTTTCGCGCTGTTCTGGCTAACCCAGGAAGCGATCCCCCTGCTa                                >  1:2237351/1‑100 (MQ=255)

                                                                 ccGGATATTGCAGACCTCACCAGCGAACAGTTCCAAAAGACCTTTGCCATTAACGTTTTCGCGCTGTTCTGGCTAACCCAGGAAGCGATCCCCCTGCTAc                               >  2:407291/1‑100 (MQ=255)

                                                                 ccGGATATTGCAGACCTCAACAGCGAACAGTTCCAAAAGACCTTTGCCATTAACGTTGTCGCGCTGTTAGGGCTAACCAAGAAAGCGATCCCCCTGCTAc                               >  2:2675601/1‑100 (MQ=255)

                                                                  cGGATATTGCAGACCTCACCAGCGAACAGTTCCAAAAGACCTTTGCCATTAACGTTTTCGCGCTGTTCTGGCTAACCCAGGAAGCGATCCCCCTGCTAc                               <  2:41469/99‑1 (MQ=255)

                                                                     atatTGCAGACCTCACCAGCGAACAGTTCCAAAAGACCTTTGCCATTAACGTTTTCGCGCTGTTCTGGCTAACCCAGGAAGCGATCCCCCTGCTACCGaa                           >  1:3012644/1‑100 (MQ=255)

                                                                      tatTGCAGTCCTCACCAGCGAAAAGTTCCAAAAGACCTTTGCCATTAACGTTTTCGCGATGTTCTGGCTAACCCAGGAAGCGATCCCCCTGCTACCGaa                           <  1:1052968/99‑1 (MQ=255)

                                                                       atTGCAGACCTCACCAGCGAACAGTTCCAAAAGACCTTTGCCATTAACGTTTTCGCGCTGTTCTGGCTAACCCAGGAAGCGATCCCCCTGCTACCGAAAg                         >  2:2686635/1‑100 (MQ=255)

                                                                        ttGCAGACCTCACCAGCGAACAGTTCCAAAAGACCTTTGCCATTAACGTTTTCGCGCTGTTCTGGCTAACCCAGGAAGCGATCCCCCTGCTACCGAAAgg                        <  2:531555/100‑1 (MQ=255)

                                                                        ttGCAGACCTCACCAGCGAACAGTTCCAAAAGACCTTTGCCATTAACGTTTTCGCGCTGTTCTGGCTAACCCAGGAAGCGATCCCCCTGCTACCGAAAgg                        <  2:2606120/100‑1 (MQ=255)

                                                                        ttGCAGACCTCACCAGCGAACAGTTCCAAAAGACCTTTGCCATTAACGTTTTCGCGCTGTTCTGGCTAACCCAGGAAGCGATCCCCCTGCTACCGAAAgg                        <  2:2076588/100‑1 (MQ=255)

                                                                        ttGCAGACCTCACCAGCGAACAGTTCCAAAAGACCTTTGCCATTAACGTTTTCGCGCTGTTCTGGCTAACCCAGGAAGCGATCCCCCTGCTACCGAAAgg                        <  2:1288616/100‑1 (MQ=255)

                                                                        ttGCAGACCTCACCAGCGAACAGTTCCAAAAGACCTTTGCCATTAACGTTTTCGCGCTGTTCTGGCTAACCCAGGAAGCGATCCCCCTGCTACCGAAAg                         >  2:1027076/1‑99 (MQ=255)

                                                                        ttGCAGACCTCACCAGCGAACAGTTCCAAAAGACCTTTGCCATTAACGTTTTCGCGCTATTCTAGCTAACACAGAAAGCGATCCCCCTGCTACCGAAAgg                        >  1:2439879/1‑100 (MQ=255)

                                                                         tGCAGACCTCACCAGCGAACAGTTCCAAAAGACCTTTGCCATTAACGTTTTCGCGCTGTTCTGGCTAACCCAGGAAGCGATCCCCCTGCTACCGAAAGGt                       >  2:83008/1‑100 (MQ=255)

                                                                         tGCAGACCTCACCAGCGAACAGTTCCAAAAGACCTTTGCCATTAACGTTTTCGCGCTGTTCTGGCTAACCCAAGAAGCGATCCCCCTGCTACCGAAAGGt                       >  2:2428890/1‑100 (MQ=255)

                                                                          gCAGACCTCACCAGCGAACAGTTCCAAAAGACCTTTGCCATTAACGTTTTCGCGCTGTTCTGGCTAACCCAGGAAGCGATCCCCCTGCTACCGAAAgg                        >  2:773295/1‑98 (MQ=255)

                                                                          gCAGACCTCACCAGCGAACAGTTCCAAAAGACCTTTGCCATTAACGTTTTCGCGCTGTTCTGGCTAACCCAGGAAGCGATCCCCCTGCTACCGAAAGGTg                      <  1:2142993/100‑1 (MQ=255)

                                                                           cAGACCTCACCAGCGAACAGTTCCAAAAGACCTTTGCCATTAACGTTTTCGCGCTGTTCTGGCTAACCCAGGAAGCGATCCCCCTGCTACCGAAAGGTGc                     >  2:2883090/1‑100 (MQ=255)

                                                                           cAGACCTCACCAGCGAACAGTTCCAAAAGACCTTTGCCATTAACGTTTTCGCGCTGTTCTGGCTAACCCAGGAAGCGATCCCCCTGCTACCGAAAGGTGc                     >  2:376143/1‑100 (MQ=255)

                                                                            aGACCTCACCAGCGAACAGTTCCAAAAGACCTTTGCCATTAACGTTTTCGCGCTGTTCTGGCTAACCCAGGAAGCGATCCCCCTGCTACCGAAAGGTGCa                    <  2:480481/100‑1 (MQ=255)

                                                                            aGACCTCACCAGCGAACAGTTCCAAAAGACCTTTGCCATTAACGTTTTCGCGCTGTTCTGGCTAACCCAGGAAGCGATCCCCCTGCTACCGAAAGGTGCa                    <  1:185489/100‑1 (MQ=255)

                                                                            aGACCTCACCAGCGAACAGTTCCAAAAGACCTTTGCCATTAACGTTTTCGCGCTGTTCTGGCTAACCCAGGAAGCGATCCCCCTGCTACCGAAAGGTGCa                    <  1:426976/100‑1 (MQ=255)

                                                                              aCCTCACCAGCGAACAGTTCCAAAAGACCTTTGCCATTAACGTTTTCGCGCTGTTCTGGCTAACCCAGGAAGCGATCCCCCTGCTACCGAAAGGTGCAAg                  <  1:2878289/100‑1 (MQ=255)

                                                                              aCCTCACCAGCGAACAGTTCCAAAAGACCTTTGCCATTAACGTTTTCGCGCTGTTCTGGCTAACCCAGGAAGCGATCCCCCTGCTACCGAAAGGTGCAAg                  <  1:83009/100‑1 (MQ=255)

                                                                               ccTCACCAGCGAACAGTTCCAAAAGACCTTTGCCATTAACGTTTTCGCGCTGTTCTGGCTAACCCAGGAAGCGATCCCCCTGCTACCGAAAGGTGCAAGt                 <  1:571775/100‑1 (MQ=255)

                                                                               ccTCACCAGCGAACAGTTCCAAAAGACCTTTGCCATTAACGTTTTCGCGCTGTTCTGGCTAAAACAGGAAGCGATCCCCCTGCTACCGAAAGGTGCAAGt                 >  1:2293074/1‑100 (MQ=255)

                                                                                cTCACCAGCGAAGAGTGGCAAAAGACCTTTGTCATTAACGTTTTCGCGCTGTTCTGGCTAACCCAGGAAGCGATCCTCCTGCTACCGAAAGGTGCAAGTa                <  2:2110151/100‑1 (MQ=255)

                                                                                  cACCAGCGAACAGTTCCAAAAGACCTTGGCCATTAACGTTTTCGCGCTGTTCTGGCTAACCCAGGAAGCGATCCTCCTGCTACCTAAAGGTGCAAGTatc              <  2:1185834/100‑1 (MQ=255)

                                                                                   aCCAGCGAACAGTTCCAAAAGACCTTTGCCATTAACGTTTTCGCGCTGTTCTGGCTAACCCAGGAAGCGATCCCCCTGCTACCGAAAGGTGCAAGTatca             <  1:1342904/100‑1 (MQ=255)

                                                                                    ccAGCGAACAGTTCCAAAAGACCTTTGCCATTAACGTTTTCGCGCTGTTCTGGCTAACCCAGGAAGCGATCCCCCTGCTACCGAAAGGTGCAAGTatcat            >  2:1508419/1‑100 (MQ=255)

                                                                                     cAGCGAACAGTTCCAAAAGACCTTTGCCATTAACGTTTTCGCGCTGTTCTGGCTAACCCAGGAAGCGATCCCCCTGCTACCGAAAGGTGCAAGTatcatc           <  2:2599266/100‑1 (MQ=255)

                                                                                     cAGCCAACAGTTCCAAAATACCTTTGCCATTAACGTTTTCGCGCTGTTCTGGCTAACCCAGGAAGCGATCCCCCTGCTACCGAAAGGTGCAAGTatcatc           <  1:2675633/100‑1 (MQ=255)

                                                                                       gCGAACAGTTCCAAAAGACCTTTGCCATTAACGTTTTCGCGCTGTTCTGGCTAACCCAGGAAGCGATCCCCCTGCTACCGAAAGGTGCAAGTATCATcac         >  2:326666/1‑100 (MQ=255)

                                                                                       gCGAACAGTTCCAAAAGACCTTTGCCATTAACGTTTTCGCGCTGTTCTGGCTAACCCAGGAAGCGATCCCCCTGCTACCGAAAGGTGCAAGTATCATcac         >  2:1530329/1‑100 (MQ=255)

                                                                                       gCGAACAGTTCCAAAAGACCTTTGCCATTAACGTTTTCGCGCTGTTCTGGCTAACCCAGGAAGCGATCCCCCTGCTACCGAAAGGAGCAAGTATCATcac         >  2:2066753/1‑100 (MQ=255)

                                                                                        cGAACAGTTCCAAAAGACCTTTGCCATTAACGTTTTCGCGCTGTTCTGGCTAACCCAGGAAGCGATCCCCCTGCTACCGAAAGGTGCAAGTATCATcacc        <  1:2333745/100‑1 (MQ=255)

                                                                                        cGAACAGTTCCAAAAGACCTTTGCCATTAACGTTTTCGCGCTGTTCTGGCTAACCCAGGAAGCGATCCCCCTGCTACCGAAAGGTGCAAGTATCATcacc        <  2:118620/100‑1 (MQ=255)

                                                                                         gAACAGTTCCAAAAGACCTTTGCCATTAACGTTTTCGCGCTGTTCTGGCTAACCCAGGAAGCGATCCCCCTGCTACCGAAAGGTGCAAGTATCATcacca       >  2:2965383/1‑100 (MQ=255)

                                                                                         gAACAGTTCCAAAAGACCTTTGCCATTAACGTTTTCGCGCTGTTCTGGCTAACCCAGGAAGCGATCCCCCTGCTACCGAAAGGTGCAAGTATCATcacca       >  2:1267736/1‑100 (MQ=255)

                                                                                          aaCAGTTCCAAAAGACCTTTGCCATTAACGTGTTCGCGCGGTTCTGGCTAACCCAGGAAGCGATCCCCCTGCTACCGAAAGGTGCAAGTATCATcacc        <  1:2891816/98‑1 (MQ=255)

                                                                                            cAGTTCCAAAAGACCTTTGCCATTAACGTTTTCGCGCTGTTCTGGCTAACCCAGGAAGCGATCCCCCTGCTACCGAAAGGTGCAAGTATCATCACCACtt    >  1:1759611/1‑100 (MQ=255)

                                                                                              gTTCCAAAAGACCTTTGCCATTAACGTTTTCGCGCTGTTCTGGCTAACCCAGGAAGCGATCCCCCTGCTACCGAAAGGTGCAAGTATCATCACCACTTCg  >  2:2861420/1‑100 (MQ=255)

                                                                                              gTTCCAAAAGACCTTTGCCATTAACGTTTTCGCGCTGTTCTGGCTAACCCAGGAAGCGATCCCCCTGCTACCGAAAGGTGCAAGTATCATCACCACTTCg  <  1:1322795/100‑1 (MQ=255)

                                                                                              gTTCCAAAAGACCTTTGCCATTAACGTTTTCGCGCTGTTCTGGCTAACCCAGGAAGCGATCCCCCTGCTACCGAAAGGTGCAAGTATCATCACCACTTCg  >  1:2575611/1‑100 (MQ=255)

AAGCGCACAAGGCGTTAGGCGGGCTGGATATTATGGCGCTGGTCGCCGGGAAACAGGTTGCCATTCCGGATATTGCAGACCTCACCAGCGAACAGTTTCAAAAGACCTTTGCCATTAACGTTTTCGCGCTGTTCTGGCTAACCCAGGAAGCGATCCCCCTGCTACCGAAAGGTGCAAGTATCATCACCACTTCG  >  NC_000913/3150023‑3150216

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`