Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,452,057 A→T 100% I77N (ATC→AAC)  rplD ← 50S ribosomal subunit protein L4

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,452,0570AT98.5% 423.4 / ‑2.2 129I77N (ATC→AAC) rplD50S ribosomal subunit protein L4
Reads supporting (aligned to +/- strand):  ref base A (2/0);  new base T (62/65);  total (64/65)
Fisher's exact test for biased strand distribution p-value = 2.44e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.27e-01

Only 100 of 129 total aligned reads displayed.

AGGATGCTTTTCAGCGCGCCGCGGTACATCTTCTTGTTAACTTTTTGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAACGCCAGATCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGCCACGGTTTTTTACCGGAACCAGTTACTTCAGCACGAGTCTTCTG  >  NC_000913/3451961‑3452151
                                                                                                |                                                                                              
aGGATGCTTTTCAGCGCGCCGCGGTACATCTTCTTGTTAACTTTTTGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAACGCCAGTTCg                                                                                             <  2:177830/100‑1 (MQ=255)
 ggATGCTTTTCAGCGCGCCGCGGTACATCTTCTTGTTAACTTTTTGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAACGCCAGTTCgg                                                                                            <  2:899827/100‑1 (MQ=255)
 ggATGCTTTTCAGCGCGCCGCGGTACATCTTCTTGTTAACTTTTTGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAACGCCAGTTCgg                                                                                            <  1:1268312/100‑1 (MQ=255)
 ggATGCTTTTCAGCGCGCCGCGGTACATCTTCTTGTTAACTTTTTGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAACGCCAGTTCgg                                                                                            <  2:1459915/100‑1 (MQ=255)
 ggATGCTTTTCAGCGCGCCGCGGTACATCTTCTTGTTAACTTTTTGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAACGCCAGTTCgg                                                                                            >  2:167889/1‑100 (MQ=255)
   aTGCTTTTCAGCGCGCCGCGGTACATCTTCTTGTTAACTTTTTGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAACGCCAGTTCGGGc                                                                                          <  1:2019986/100‑1 (MQ=255)
   aTGCTTTTCAGCGCGCCGCGGTACATCTTCTTGTTAACTTTTTGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAACGCCAGTTCGGGc                                                                                          <  1:1963926/100‑1 (MQ=255)
      cTTTTCAGCGCGCCGCGGTACATCTTCTTGTTAACTTTTTGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAACGCCAGTTCGGGctct                                                                                       >  1:2450615/1‑100 (MQ=255)
       ttttCAGCGCGCCGCGGTACATCTTCTTGTTAACTTTTTGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAACGCCAGTTCGGGCTCtt                                                                                      <  1:1400487/100‑1 (MQ=255)
        tttCAGCGCGCCGCGGTACATCTTCTTGTTAACTTTTTGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAACGCCAGTTCGGGCTCTTg                                                                                     <  2:640138/100‑1 (MQ=255)
          tCAGCGCGCCGCGGTACATCTTCTTGTTAACTTTTTGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAACGCCAGTTCGGGCTCTTGAt                                                                                   <  2:2916632/100‑1 (MQ=255)
          tCAGCGCGCCGCGGTACATCTTCTTGTTAACTTTTTGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAACGCCAGTTCGGGCTCTTGAt                                                                                   <  1:744521/100‑1 (MQ=255)
             gcgcgcCGCGGTACATCTTCTTGTTAATTTTTTGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAACGCCAGTTCGGGCTCTTGATAGa                                                                                <  1:2592977/100‑1 (MQ=255)
              cgcgcCGCGGTACATCTTCTTGTTAACTTTTTGACTGTGGTCCTGCGGTCGAGCAGCAAAGGTCACGCCACCAGAACGCCAGTTCGGGCTCTTGATAGa                                                                                <  1:959403/99‑1 (MQ=255)
              cgcgcCGCGGTACATCTTCTTGTTAACTTTTTGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAACGCCAGTTCGGGCTCTTGATAGaa                                                                               >  1:1771915/1‑100 (MQ=255)
               gcgcCGCGGTACATCTTCTTGTTAACTTTTTGGCTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAACGCCAGTTCGGGCTCTTGATAGAAc                                                                              <  1:2953060/100‑1 (MQ=255)
               gcgcCGCGGTACATCTTCTTGTTAACTTTTTGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAACGCCAGTTCGGGCTCTTGATAGAAc                                                                              <  2:2733146/100‑1 (MQ=255)
               gcgcCGCGGTACATCTTCTTGTTAACTTTTTGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAACGCCAGTTCGGGCTCTTGATAGAAc                                                                              <  2:2431748/100‑1 (MQ=255)
               gcgcCGCGGTACATCTTCTTGTTAACTTTTTGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAACGCCAGTTCGGGCTCTTGATAGAAc                                                                              >  1:2180458/1‑100 (MQ=255)
                cgccgcGGTACATCTTCTTGTTAACTTTTTGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAACGCCAGTTCGGGCTCTTGATAGAAcc                                                                             >  1:1323380/1‑100 (MQ=255)
                cgccgcGGTACATCTTCTTGTTAACTTTTTGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAACGCCAGTTCGGGCTCTTGATAGAAcc                                                                             <  2:141959/100‑1 (MQ=255)
                cgccgcGGTACATCTTCTTGTTAACTTTTTGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAACGCCAGTTCGGGCTCTTGATAGAAcc                                                                             <  1:594136/100‑1 (MQ=255)
                    gcgGTACATCTTCTTGTTAACTTTTTGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAACGCCAGTTCGGGCTCTTGATAGAACCAGaa                                                                         >  2:2743407/1‑100 (MQ=255)
                    gcgGTACATCTTCTTGTTAACTTTTTGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAACGCCAGTTCGGGCTCTTGATAGAACCAGaa                                                                         <  1:1943255/100‑1 (MQ=255)
                      ggTACATCTTCTTGTTAACTTTTTGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAACGCCAGTTCGGGCTCTTGATAGAACCAGAAcg                                                                       <  1:597000/100‑1 (MQ=255)
                        tACATCTTCTTGTTAACTTTTTGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAACGCCAGTTCGGGCTCTTGATAGAACCAGAAcgcg                                                                     <  1:1800377/100‑1 (MQ=255)
                        tACATCTTCTTGTTAACTTTTTGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAACGCCAGTTCGGGCTCTTGATAGAACCAGAAcgcg                                                                     <  1:2254748/100‑1 (MQ=255)
                           aTCTTCTTGTTAACTTTTTGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAACGCCAGTTCGGGCTCTTGATAGAACCAGAACGCGCAc                                                                  >  1:2873217/1‑100 (MQ=255)
                                 ttGTTAACTTTTTGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAACGCCAGTTCGGGCTCTTGATAGAACCAGAACGCGCACGGCCgg                                                            >  1:1761315/1‑100 (MQ=255)
                                   gTTAACTTTTTGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAACGCCAGTTCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTg                                                          >  2:886066/1‑100 (MQ=255)
                                   gTTAACTTTTTGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAACGCCAGTTCGGGCTCTTAATAGAACCAGAACGCGCACGGCCGGt                                                           >  1:2381788/1‑99 (MQ=255)
                                    ttAACTATTTGACTGTGTAACAGCGGAAGAGCAGCAAAGGAAACGCAACTAGAACGCAAATTCGGGATCTTGATAGAACCAGAACTCGCAcagacagaac                                                         >  2:2360107/1‑91 (MQ=255)
                                     tAACTTTTTTACTGTGGTCCTGCTGACGTGCAGCAAAGGTCACGCCACCAGAACGCCAGTTCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGcc                                                        <  1:1953993/100‑1 (MQ=255)
                                     tAACTTTTTGACTGTGGTCCTGCGGACGAGCAGCAAATGTCACGCCACCAGAACGCCAGTTCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGcc                                                        <  1:1621708/100‑1 (MQ=255)
                                     tAACTTTTTGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAACGCCAGTTCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTgac                                                        >  2:1843261/1‑98 (MQ=255)
                                     tAACTTTTTGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAACGCCAGTTCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGcc                                                        <  2:1868446/100‑1 (MQ=255)
                                     tAACTTTTTGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAACGCCAGTTCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGcc                                                        >  1:2823858/1‑100 (MQ=255)
                                        cTTTTTGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAACGCCAGTTCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCttt                                                     >  1:1252622/1‑100 (MQ=255)
                                          ttttGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAACGCCAGTTCGTGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCt                                                   <  2:750375/100‑1 (MQ=255)
                                             tGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAACGCCAGTTCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGgc                                                <  1:2501999/100‑1 (MQ=255)
                                             tGACTGTGGTCCTGCGGACGAGCAGCAAAGATCGCGCCACCAGAACGCCAGTTCGGGCTCTTGATAGAACAAGAACGCGCACGGCCGGTACCTTTCTgg                                                 >  2:1989295/1‑99 (MQ=255)
                                                cTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAACGCCAGTTCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGcc                                             >  1:2823414/1‑100 (MQ=255)
                                                cTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAACGCCAGTTCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGcc                                             <  1:1339961/100‑1 (MQ=255)
                                                cTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAACGCCAGTTCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGcc                                             <  2:1223252/100‑1 (MQ=255)
                                                  gtgGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAACGCCAGTTCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGCCAc                                           <  1:2327762/100‑1 (MQ=255)
                                                  gtgGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAACGCCAGTTCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGCCAc                                           >  1:2006967/1‑100 (MQ=255)
                                                    ggTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAACGCCAGTTCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGCCACgg                                         >  1:1896479/1‑100 (MQ=255)
                                                    ggTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAACGCCAGTTCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGCCACgg                                         >  2:1526021/1‑100 (MQ=255)
                                                    ggTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAACGCCAGTTCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGCCACgg                                         <  2:1252610/100‑1 (MQ=255)
                                                    ggTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAACGCCAGTTCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGCCACgg                                         >  1:1580176/1‑100 (MQ=255)
                                                      tCCTGCGGACGAGCAGCAAAGGTCGCGCCACCAGAACGCCAGTTCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGCCACGGtt                                       <  2:573289/100‑1 (MQ=255)
                                                       ccTGCGGCCGAGCAGCAAAGGTCACGCCACCAGAACGCCAGTTCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGCCACGGttt                                      >  2:191525/1‑100 (MQ=255)
                                                        cTGCGGACGAGCAGCAAAGGTCACGCCACCAGAACGCCAGTTCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGCCACGGtttt                                     <  1:469279/100‑1 (MQ=255)
                                                        cTGCGGACGAGCAGCAAAGGTCACGCCACCAGAACGCCAGTTCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGCCACGGtttt                                     >  1:1923989/1‑100 (MQ=255)
                                                        cTGCGGACGAGCAGCAAAGGTCACGCCACCAGAACGCCAGTTCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGCCACGGtttt                                     <  1:477210/100‑1 (MQ=255)
                                                        cTGCGGACGAGCAGCAAAGGTCACGCCACCAGAACGCCAGTTCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGCCACGGtttt                                     >  1:403538/1‑100 (MQ=255)
                                                         tgcagagcagcagcAAAGCTCACGCCACCATAACGCCAGTTCTGGCTCTTTATATAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGCCACGGtttt                                     <  2:2823382/91‑1 (MQ=255)
                                                         tGCGGACGAGCAGCAAAGGTCACGCCACCAGAACGCCAGTTCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGCCACGGttttt                                    >  2:2582664/1‑100 (MQ=255)
                                                         tGCGGACGAGCAGCAAAGGTCACGCCACCAGAACGCCAGTTCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGCCACGGttttt                                    >  2:94401/1‑100 (MQ=255)
                                                           cgtaCGAGCAGCAAAGGTCACGCCACCAGAACGCCAGTTCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGCCACGGttttt                                    <  2:2858785/95‑1 (MQ=255)
                                                             gACGAGCAGCAAAGGTCACGCCACCAGAACGCCAGTTCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGCCACGGTTTTTTAcc                                <  2:1761295/100‑1 (MQ=255)
                                                             gACGAGCAGCAAAGGTCACGCCACCAGAACGCCAGTTCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGCCACGGTTTTTTAcc                                <  2:375729/100‑1 (MQ=255)
                                                              aCGAGCAGCAAAGGTCACGCCACCGGACCGCCAGTTCCGGCTCTTGATAGAACCAGAACGCGCACGGCCGCTGCCTTTCTGGCGCCACGGtttt                                     <  2:1958061/94‑1 (MQ=255)
                                                               cGAGCAGCAAAGGTCACGCCACCAGAACGCCAGTTCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGCCACGGTTTTTTACCgg                              <  2:3000780/100‑1 (MQ=255)
                                                               cGAGCAGCAAAGGTCACGCCACCAGAACGCCAGTTCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGCCACGGTTTTTTACCgg                              <  2:418513/100‑1 (MQ=255)
                                                               agAGCAGCAAAGATCACGCCACCAGAACGCCAATTCGGGCTCTTGCTAGAACCAGAACGCGCACGCCCGGTGCATTTCTAGCCCCACGGTTTTTTAc                                 >  2:1366566/2‑97 (MQ=255)
                                                                gAGCAGCAAAGGTCACGCCACCAGAACGCCAGTTCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGCCACGGTTTTTTACCGGa                             >  2:2737824/1‑100 (MQ=255)
                                                                 agcagcAAAGGTCACTCCACCAGAACGCCAGTTCGGGCTCTTGATAGAACCATAACGCGCACGGCAGGTGCATTTCTGGCGCCACGGTTTTGTACCGGaa                            >  2:3024422/1‑100 (MQ=255)
                                                                 agcagcAAAGGTCACGCCACCAGAACGCCAGTTCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGCCACGGTTTTTTACCGGaa                            >  1:1421358/1‑100 (MQ=255)
                                                                  gcagcaAAGGTCACGCCACCAGAACGCCAGTTCGGGCTCTTGATAGAACCAGAACGCGCACGACCGGTACCTTTCTGGCGCCACGGTTTTTTACCGGAAc                           >  1:147555/1‑100 (MQ=255)
                                                                  gcagcaAAGGTCACGCCACCAGAACGCCAGTTCGGGCTCTTGATAGAACCAGAACGCACACGGCCGGTGCCTTTCTGGCGCCACGGTTTTTTACCGGAAc                           >  1:1015952/1‑100 (MQ=255)
                                                                   cagcaAAGGTCACGCCACCAGAACGCCAGTTCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGCCACGGTTTTTTACCGGAAcc                          >  1:1013016/1‑100 (MQ=255)
                                                                   cagcaAAGGTCACGCCACCAGAACGCCAGTTCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGCCACGGTTTTTTACCGGAAcc                          >  1:881409/1‑100 (MQ=255)
                                                                    agcaAAAGTCACTTCACCAAAACGCCAGTTCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGCCACGGTTTTTTACCGGAAcc                          >  2:1705383/1‑99 (MQ=255)
                                                                     gcaAAGGTCACGCCACCAGAACGCCAGTTCGGGCTCTTGATAGAACCAGAGCGCGCACGGTCGGTGCCTTTCTGGCGCCACGGTTTTTTACCGGAACCAg                        <  2:455228/100‑1 (MQ=255)
                                                                     gcaAAGGTCACGCCACCAGAACGCCAGTTCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGCCACGGTTTTTTACCGGAACCAg                        >  1:1914522/1‑100 (MQ=255)
                                                                       aaaGGTCACGCCACCAAAACGCCAGTTCGGGCTCTTGATAGAACCAGCAAGCGCACGGCCAGTGCCTTTCTGGCGCCACGGTCTTTTACCGGAACCa                         >  2:436316/1‑97 (MQ=255)
                                                                         aGGTCACGCCACCAGAACGCCAGTTCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGACACGGTTTTTTACCGGAACCAGTTAc                    <  1:1843282/100‑1 (MQ=255)
                                                                          ggTCACGCCACCAGAACGCCAGTTCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGCCACGGTTTTTTACCGGAACCAGTTACt                   >  1:1039759/1‑100 (MQ=255)
                                                                          ggTCACGCCACCAGAACGCCAGATCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGCCACGGTTTTTTACCGGAACCAGTTACt                   >  2:2343707/1‑100 (MQ=255)
                                                                            tCACGCCACCAGAACGCCAGTTCGGTCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGCCACGGTTTTTTACCGGAACCAGTTACTTc                 <  2:653058/100‑1 (MQ=255)
                                                                            tCACGCCACCAGAACGCCAGTTCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGCCACGGTTTTTTACCGGAACCAGTTACTTc                 <  2:2873185/100‑1 (MQ=255)
                                                                            tCACGCCACCAGAACGCCAGTTCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGCCACGGTTTTTTACCGGAACCAGTTACTTc                 <  2:2399557/100‑1 (MQ=255)
                                                                            tCACGCCACCAGAACGCCAGTTCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGCCACGGTTTTTTACCGGAACCAGTTACTTc                 <  2:1601882/100‑1 (MQ=255)
                                                                             cACGCCACCAGAACGCCAGTTCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGCCACGGTTTTTTACCGGAACCAGTTACTTCa                >  2:1741533/1‑100 (MQ=255)
                                                                             cACGCCACCAGAACGCCAGTTCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGACACGGTTTTTTACCGGAACCAGTTACTTCa                >  2:2555546/1‑100 (MQ=255)
                                                                               cGCCACCAGAACGCCAGTTCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGCCACGGTTTTTTACCGGAACCAGTTACTTCAGc              <  1:598833/100‑1 (MQ=255)
                                                                                gCCACCAGAACGCCAGTTCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGCCACGGTTTTTTACCGGAACCAGTTACTTCAGCa             <  2:1072810/100‑1 (MQ=255)
                                                                                gCCACCAGAACGCCAGTTCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGCCACGGTTTTTTACCGGAACCAGTTACTTCAGCa             <  1:2270212/100‑1 (MQ=255)
                                                                                 ccaccaGAACGCCAGTTCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGCCACGGTTTTTTACCGGAACCAGTTACTTCAGCAc            >  1:384126/1‑100 (MQ=255)
                                                                                  aaccaGAACGCCAGTTCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGCCACGGTTTTTTACCGGAACCAGTTACTTCAGCACg           >  2:3033511/2‑100 (MQ=255)
                                                                                   accaGAACGCCAGTTCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGCCACGGTTTTTTACCGGAACCAGTTACTTCAGCACGa          >  1:901723/1‑100 (MQ=255)
                                                                                    ccaGAACGCCAGTTCGGTCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCTCCACGGTTTTTTACCGGAACCAGTTACTTCAGCACGa          >  2:2578540/1‑99 (MQ=255)
                                                                                     caGAACGCCAGTTCGGGCTCTTGATAGAAGCAGAACGCGCACGGCCGGTGCCTTTCTGGCGCCACGGTTTTTTACCGGAATCAGTTACTTCAGCACGAGt        <  2:2089173/100‑1 (MQ=255)
                                                                                      aGAACGCCAGTTCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGCCACGGTTTTTTACCGGAACCAGTTACTTCAGCACGAGtc       <  2:978499/100‑1 (MQ=255)
                                                                                          cGCCAGTTCGGGCTCTTGATAGAACCAGTACGCGCACGGCCGGTGCCTTTCTGGCGCCACGGTTTTTTACCGGAACCAGTTACTTCAGCACGAGtcttc    <  1:2594792/99‑1 (MQ=255)
                                                                                          cGCCAGTTCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGCCACGGTTTTTTACTTGAATCAGTTACTTCAGCACGAGtcttct   >  2:1896824/1‑100 (MQ=255)
                                                                                          cGCCAGTTCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGCCACGGTTTTTTACCGGCACCAGTTACTTCAGCACGAGtcttct   >  2:970830/1‑100 (MQ=255)
                                                                                           gCCAGTTCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGCCACGGTTTTTTACCGGAACCAGTTACTTCAGCACGAGTCTTCTg  <  1:1031865/100‑1 (MQ=255)
                                                                                           gCCAGTTCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGCCACGGTTTTTTACCGGAACCAGTTACTTCAGCACGAGTCTTCTg  >  2:91483/1‑100 (MQ=255)
                                                                                                |                                                                                              
AGGATGCTTTTCAGCGCGCCGCGGTACATCTTCTTGTTAACTTTTTGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAACGCCAGATCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGCCACGGTTTTTTACCGGAACCAGTTACTTCAGCACGAGTCTTCTG  >  NC_000913/3451961‑3452151

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: