Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,477,434 T→C 100% intergenic (‑15/‑206) fkpA ← / → slyX FKBP‑type peptidyl‑prolyl cis‑trans isomerase (rotamase)/phi X174 lysis protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,477,4340TC98.1% 332.0 / ‑2.6 102intergenic (‑15/‑206)fkpA/slyXFKBP‑type peptidyl‑prolyl cis‑trans isomerase (rotamase)/phi X174 lysis protein
Reads supporting (aligned to +/- strand):  ref base T (2/0);  new base C (41/59);  total (43/59)
Fisher's exact test for biased strand distribution p-value = 1.75e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.41e-01

Only 100 of 102 total aligned reads displayed.

GCTTCAGCAGCAAAAGTGATTGGTGCATGCAGGGCAACGGCCATTGTGGTCGCCAGCAGCGTTACTTTAAACAGTGATTTCATCCATATCTCCAGGATCGGGGCATCTCACCCCAGGGTTAACTATTATCAGAAGGGTACTATAAAGCGTTGTCGAACAAATCTACATACAGACACGCCCTATTATCATCTATT  >  NC_000913/3477337‑3477530
                                                                                                 |                                                                                                
gCTTCAGCAGCAAAAGTGATTGGTGCATGCAGGGCAACGGCCATTGTGGTCGCCAGCAGCGTTACTTTAAACAGTGATTTCATCCATATCTCCAGGACCg                                                                                                <  2:2708010/100‑1 (MQ=255)
   tCAGCAGCAAAAGTGATTGGTGCATGCAGGGCAACGGCCATTGTGGTCGCCAGCAGCGTTACTTTAAACAGTGATTTCATCCATATCTCCAGGACCgggg                                                                                             <  2:882473/100‑1 (MQ=255)
     agcagcAAAAGTGATTGGTGCATGCAGGGCAACGGCCATTGTGGTCGCCAGCAGCGTTACTTTAAACAGTGATTTCATCCATATCTCCAGGACCGGGGCa                                                                                           >  1:940675/1‑100 (MQ=255)
     agcagcAAAAGTGATTGGTGCATGCAGGGCAACGGCCATTGTGGTCGCCAGCAGCGTTACTTTAAACAGTGATTTCATCCATATCTCCAGGACCGGGGCa                                                                                           <  1:235275/100‑1 (MQ=255)
       cagcaAAAGTGATTGGTGCATGCAGTGTTACGGCCATTGTGGTTGTCAGCAGCGTTACTTTAAACAGTGATTTCATCCATATCTCCAGGACCGGGGCAtc                                                                                         <  1:163/100‑1 (MQ=255)
       cagcaAAAGTGATTGGTGCATGCAGGGCAACGGCCATTGTGGTCGCCAGCAGCGTTACTTTAAACAGTGATTTCATCCATATCTCCAGGACCGGGGCAtc                                                                                         >  2:1394037/1‑100 (MQ=255)
        agcaAAAGTGATTGGTGCATGCAGGGCAACGGCCATTGTGGTCGCCAGCAGCGTTACTTTAAACAGTGATTTCATCCATATCTCCAGGACCGGGGCAtct                                                                                        <  2:2551998/100‑1 (MQ=255)
         gcaAAAGTGATTGGTGCATGCAGGGCAATGGCCATTGTGGTCGCCAGCATCGTTACTTTAAACAGTGATTTCATCCATATCTCCAGGACCGGGGCAtct                                                                                        <  2:399128/99‑1 (MQ=255)
         gcaAAAGTGATTGGTGCATGCAGGGCAACGGCCATTGTGGTCGCCAGCAGCGTTACTTTAAACAGTGATTTCATCCATATCTCCAGGACCGGGGCAtctc                                                                                       >  1:2287845/1‑100 (MQ=255)
             aaGTGATTGGTGCATGCAGGGCAACGGCCATTGTGGTCGCCAGCAGCGTTACTTTAAACAGTGATTTCATCCATATCTCCAGGACCGGGGCATCTCAccc                                                                                   <  2:1296155/100‑1 (MQ=255)
              aGTGATTGGTGCATGCAGGGCAACGGCCATTGTGGTCGCCAGCAGCGTTACTTTAAACAGTGATTTCATCCATATCTCCAGGACCGGGGCATCTCAcccc                                                                                  <  1:2026848/100‑1 (MQ=255)
              aGTGATTGGTGCATGCAGGGCAACGGCCATTGTGGTCGCCAGCAGCGTTACTTTAAACAGTGATTTCATCCATATCTCCAGGACCGGGGCATCTCAcccc                                                                                  >  1:2445965/1‑100 (MQ=255)
              aGTGATTGGTGCATGCAGGGCAACGGCCATTGTGGTCGCCAGCAGCGTTACTTTAAACAATGATTTCAACCATATCACCAGGACCGGGGAATCTCAcccc                                                                                  >  1:1829768/1‑100 (MQ=255)
               gTGATTGGTGCATGCAGGGCAACGGCCATTGTGGTCGCCAGCAGCGTTACTTTAAACAGTGATTTCATCCATATCTCCAGGACCGGGGCATCTCACCCCa                                                                                 >  1:2238288/1‑100 (MQ=255)
                 gATTGGTGCATGCAGGGCAACGGCCATTGTGGTCGCCAGCAGCGTTACTTTAAACAGTGATTTCATCCATATCTCCAGGACCGGGGCATCTCACCCCAgg                                                                               <  1:1675137/100‑1 (MQ=255)
                   ttGGTGCATGCAGGGCAACGGTCATTGTTGTTGTTAGTAGTGTTACTTTAAACAGTGATTTCATCCATATCTCCAGGACCGGGGCATCTCACCCCAGGGt                                                                             <  1:1266813/100‑1 (MQ=255)
                   ctGGTGCATGCAGGGCAACGGCCATTGTGGTCGCCAGCAGCGTTACTTTAAACAGTGATTTCATCCATATCTCCAGGACCGGGGCATCTCACCCCAGGGt                                                                             <  2:1373175/99‑1 (MQ=255)
                    tGGTGCATGCAGGGCAACGGCCATTGTGGTCGCCAGCAGCGTTACTTTAAACAGTGATTTCATCCATATCTCCAGGACCGGGGCATCTCACCCCAGGGtt                                                                            <  1:400456/100‑1 (MQ=255)
                     ggTGCATGCAGGGCAACGGCCATTGTGGTCGCCAGCAGCGTTACTTTAAACAGTGATTTCATCCATATCTCCAGGACCGGGGCATCTCACCCCAGGGTTa                                                                           <  2:2540291/100‑1 (MQ=255)
                     ggTGCATGCAGGGCAACGGCCATTGTGGTCGCCAGCAGCGTTACTTTAAACAGTGATTTCATCCATATCTCCAGGACCGGGGCATCTCACCCCAGGGTTa                                                                           <  2:2539845/100‑1 (MQ=255)
                          atgcaGGGCAACGGCCATTGTGGTCGCCAGCAGCGTTACTTTAAACAGTGATTTCATCCATATCTCCAGAACCGGGGCATCTCACCCCAGGGTTAACtat                                                                      >  1:564889/1‑100 (MQ=255)
                           tgcaGGGCAACGGCCATTGTGGTCGCCAGCAGCGTTACTTTAAACAGTGATTTCATCCATATCTCCAGGACCGGGGCATCTCACCCCAGGGTTAACtatt                                                                     >  2:1686581/1‑100 (MQ=255)
                           tgcaGGGCAACGGCCATTGTGGTCGCCAGCAGCGTTACTTTAAACAGTGATTTCATCCATATCTCCAGGACCGGGGCATCTCACCCCAGGGTTAACtatt                                                                     <  1:1650360/100‑1 (MQ=255)
                           tgcaGGGCAACGGCCATTGTGGCCGCCAGCACCGTTACTTTAAACAGCGATTTCATCCATATCTCCAGGACCGGGGCATCTCACCCCAGGGTTAACtatt                                                                     <  2:2046998/100‑1 (MQ=255)
                           tgaaGGGCAACGGCCATTGTGGTCGCCAGCAGCGTTACTTTAAACAGTGATATCATCCATATCTCCAGGACCGGGGCATCTCACCCCAGGGTTAACtat                                                                      >  2:1692812/4‑99 (MQ=255)
                              aGGGCAACGGCCATTGTGGTCGCCAGCAGCGTTACTTTAAACAGTGATTTCATCCATATCTCCAGGTCCGGGGCATCTCACCCCAGGGTTAACTATTATc                                                                  <  2:2003737/100‑1 (MQ=255)
                              aGGGCAACGGCCATTGTGGTCGCCAGCAGCGTTACTTTAAACAGTGATTTCATCCATATCTCCAGGACCGGGGCATCTCACCCCAGGGTTAACTATTATc                                                                  <  1:1019444/100‑1 (MQ=255)
                               gggCAACGGCCATTGTGGTCGCCAGCAGCGTTACTTTAAACAGTGATTTCATCCATATCTCCAGGACCGGGGCATCTCACCCCAGGGTTAACTATTATCa                                                                 >  1:1973662/1‑100 (MQ=255)
                               gggCAACGGCCATTGTGGTCGCCAGCAGCGTTACTTTAAACAGTGATTTCATCCATATCTCCAGGACCGGGGCATCTCACCCCAGGGTTAACTATTATCa                                                                 <  2:1356815/100‑1 (MQ=255)
                               gggCAACGGCCAGTGTGGTCGCCAGCAGCGTTACTTTAAACAGTGATTTCATCCATATCTCCAGGATCGGGGCATCTCACCCCAGGGTTAACTATTATCa                                                                 >  1:1991399/1‑100 (MQ=255)
                                 gCAACGGCCATTGTGGTCGCCAGCAGCGTTACTTTAAACAGTGATTTCATCCATATCTCCAGGACCGGGGCATCTCACCCCAGGGTTAACTATTATCAGa                                                               >  2:3019479/1‑100 (MQ=255)
                                   aaCGGCCATTGTGGTCGCCAGCAGCGTTACTTTAAACAGTGATTTCATCCATATCTCCAGGACCGGGGCATCTCACCCCAGGGTTAACTATTATCAGAAg                                                             <  2:1922077/100‑1 (MQ=255)
                                     cGGCCATTGTGGTCGCCAGCAGCGTTACTTTAAACAGTGATTTCATCCATATCTCCAGGACCGGGGCATCTCACCCCAGGGTTAACTATTATCAGAAggg                                                           <  1:1330115/100‑1 (MQ=255)
                                         cATTGTGGTCGCCAGCAGCGTTACTTTAAACAGTGATTTCATCCATATCTCCAGGACCGGGGCATCTCACCCCAGGGTTAACTATTATCAGAAGGGTACt                                                       >  2:2444363/1‑100 (MQ=255)
                                         cATTGTGGTCGCCAGCAGCGTTACTTTAAACAGTGATTTCATCCATATCTCAAGGACCAGAGCATCTCAACCCAGGGTTAACTATTATCAGAAGGGTACt                                                       >  1:919831/1‑100 (MQ=255)
                                           ttGTGGTCGCCAGCAGCGTTACTTTAAACAGTGATTTCATCCATATCTCCAGGACCGGGGCATCTCACCCCAGGGTTAACTATTATCAGAAGGGTACtat                                                     <  1:307983/100‑1 (MQ=255)
                                           ttGTGGTCGCCAGCAGCGTTACTTTAAACAGTGATTTCATCCATATCTCCAGGACCGGGGCATCTCACCCCAGGGTTAACTATTATCAGAAGGGTACtat                                                     <  1:307953/100‑1 (MQ=255)
                                             gtgGTCGCCAGCAGCGTTACTTTAAACAGTGATTTCATCCATATCTCCAGGACCGGGGCATCTCACCCCAGGGTTAACTATTATCAGAAGGGTACTATaa                                                   >  2:2870707/1‑100 (MQ=255)
                                               ggTCGCCAGCAGCGTTACTTTAAACAGTGATTTCATCCATATCTCCAGGACCGGGGCATCTCACCCCAGGGTTAACTATTATCAGAAGGGTACTATAAAg                                                 >  2:1964164/1‑100 (MQ=255)
                                               ggTCGCCAGCAGCGTTACTTTAAACAGTGATTTCATCCATATCTCCAGGACCGGGGCATCTCACCCCAGGGTTAACTATTATCAGAAGGGTACTATAAAg                                                 <  1:1774747/100‑1 (MQ=255)
                                               ggTCGCCAGCAGCGTTACTTTAAACAGTGATTTCATCCATATCTCCAGGACCGGGGCATCTCACCCCAGGGTTAACTATTATCAGAAGGGTACTATAAAg                                                 <  1:1774798/100‑1 (MQ=255)
                                                ntCGCCAGCAGCGTTACTTTAAACAGTGATTTCATCCATATCTCCAGGACCGGGGCATCTCACCCCAGGGTTAACTATTATCAGAAGGGTACTATAAAGc                                                >  2:1028227/2‑100 (MQ=255)
                                                gTCGCCAGCAGCGTTACTTTAAACAGTGATTTCATCCATATCTCCAGGACCGGGGCATCTCACCCCAGGGTTAACTATTATCAGAAGGGTACTATAAAGc                                                >  1:2545133/1‑100 (MQ=255)
                                                gTCGCCAGCAGCGTTACTTTAAACAGTGATTTCATCCATATCTCCAGGACCGGGGCATCTCACCCCAGGGTTAACTATTATCAGAAGGGTACTATAAAGc                                                >  1:1175718/1‑100 (MQ=255)
                                                gTCGCCAGCAGCGTTACTTTAAACAGTGATTTCATCCATATCTCCAGGACCGGGGCATCTCACCCCAGGGTTAACTATTATCAGAAGGGTACTATAAAGc                                                >  1:1868239/1‑100 (MQ=255)
                                                  cGCTAGCAGCGTTACTTTAAACATTAAACACATCGATAACGCCATTACCGGGTCATCGCACGTCAGGGGGAACTTTTATCGTAGGGGTACTATAATGc                                                >  2:1003456/1‑98 (MQ=255)
                                                  cGCCAGCAGCGTTACTTTAAACAGTGATTTCATCCATATCTCCAGGACCGGGGCATCTCACCCCAGGGTTAACTATTATCAGAAGGGTACTATAAAGCGt                                              <  2:199771/100‑1 (MQ=255)
                                                  cGCCAGCAGCGTTACTTTAAACAGTGATTTCATCCATATCTCCAGGACCGGGGCATCTCACCCCAGGGTTAACTATTATCAGAAGGGTACTATAAAGCGt                                              <  2:825958/100‑1 (MQ=255)
                                                   gCCAGCAGCGTTACTTTAAACAGTGATTTCATCCATATCTCCAGGACCGGGGCATCTCACCCCAGGGTTAACTATTATCAGAAGGGTACTATAAAGCGt                                              <  2:902444/99‑1 (MQ=255)
                                                    ccAGCAGCGTTACTTTAAACAGTGATTTCATCCATATCTCCAGGACCGGGGCATCTCACCCCAGGGTTAACTATTATCAGAAGGGTACTATAAAGCGTTg                                            <  1:1686601/100‑1 (MQ=255)
                                                    ccAGCAGCGTTACTTTAAACAGTGATTTCATCCATATCTCCAGGACCGGGGCATCTCACCCCAGGGTTAACTATTATCAGAAGGGTACTATAAAGCGTTg                                            <  2:747027/100‑1 (MQ=255)
                                                     cagcagCGTTACTTTAAACAGTGATTTCATCCATATCTCCAGGACCGGGGCATCTCACCCCAGGGTTAACTATTATCAGAAGGGTACTATAAAGCGTTGt                                           <  1:1426232/100‑1 (MQ=255)
                                                      agcagcGTTACTTTAAACAGTGATTTCATCCATATCTCCAGGACCGGGGCATCTCACCCCAGGGTTAACTATTATCAGAAGGGTACTATAAAGCGTTGTc                                          <  1:1746433/100‑1 (MQ=255)
                                                       gcagcGTTACTTTAAACAGTGATTTCATCCATATCTCCAGGACCGGTGCATCTCACCCCAGGGTTAACTATTATCAGAAGGGTACTATAAAGCGTTGTCg                                         <  2:516145/100‑1 (MQ=255)
                                                          gcGTTACTTTAAACAGTGATTTCATCCATATCTCCAGGACCGGGGCATCTCACCCCAGGGTTAACTATTATCAGAAGGGTACTATAAAGCGTTGTCGAAc                                      <  1:2456253/100‑1 (MQ=255)
                                                           cGTTACTTTAAACAGTGATTTCATCCATATCTCCAGGACCGGGGCATCTCACCCCAGGGTTAACTATTATCAGAAGGGTACTATAAAGCGTTGTCGAACa                                     <  2:2163355/100‑1 (MQ=255)
                                                            gTTACTTTAAACAGTGATTTCATCCATATCTCCAGGACCGGGGCATCTCACCCCAGGGTTAACTATTATCAGAAGGGTACTATAAAGCGTTGTCGAACaa                                    >  1:1814106/1‑100 (MQ=255)
                                                                 tttAAACAGTGATTTCATCCATATCTCCAGGACCGGGGCATCTCACCCCAGGGTTAACTATTATCAGAAGGGTACTATAAAGCGTTGTCGAACAAATCta                               <  2:1495410/100‑1 (MQ=255)
                                                                  ttAAACAGTGATTTCATCCATATCTCCAGGACCGGGGCATCTCACCCCAGGGTTAACTATTATCAGAAGGGTACTATAAAGCGTTGTCGAACAAATCtan                              <  1:1028234/100‑2 (MQ=255)
                                                                  ttAAACAGTGATTTCATCCATATCTCCAGGACCGGGGCATCTCACCCCAGGGTTAACTATTATCAGAAGGGTACTATAAAGCGTTGTCGAACAAATCtac                              >  1:2884502/1‑100 (MQ=255)
                                                                   tAAACAGTGATTTCATCCATATCTCCAGTTCCGGGGCATCTCACCCAAGGGTTAACTATTATCAGAAGGGTACTATAAAGCGTTGTCGAACAAATCtaca                             <  1:47536/100‑1 (MQ=255)
                                                                    aaaCAGTGATTTCATCCATATCTCCAGGACCGGGGCATCTCACCCCAGGGTTAACTATTATCAGAAGGGTACTATAAAGCGTTGTCGAACAAATCtacat                            >  2:1111495/1‑100 (MQ=255)
                                                                    aaaCAGTGATTTCATCCATATCTCCAGGACCGGGGCATCTCACCCCAGGGTTAACTATTATCAGAAGGGTACTATAAAGCGTTGTCGAACAAATCtacat                            >  1:2429956/1‑100 (MQ=255)
                                                                     aaCAGTGATTTCATCCATATCTCCAGGATCGGGGCATCTCACCCCAGGGTTAACTATTATCAGAAGGGTACTATAAAGCGTTGTCGAACAAATCtacata                           >  1:3005621/1‑100 (MQ=255)
                                                                     aaCAGTGATTTCATCCATATCTCCAGGACTGGGGCATCTCACCCCAGGGTTAACGATTATCAGAAGGGTACTATAAAGCGTTGTCGAACAAATCtacat                            <  1:3038175/99‑1 (MQ=255)
                                                                     aaCAGTGATTTCATCCATATCTCCAGGACCGGGGCATCTCACCCCAGGGTTAACTATTATCAGAAGGGTACTATAAAGCGTTGTCGAACAAATCtacata                           <  1:785252/100‑1 (MQ=255)
                                                                     aaCAGTGATTTCATCCATATCTCCAGGACCGGGGCATCTCACCCCAGGGTTAACTATTATCAGAAGGGTACTATAAAGCGTTGTCGAACAAATCtacata                           >  1:58294/1‑100 (MQ=255)
                                                                      aCAGTGATTTCATCCATATCTCCAGGACCGGGGCATCTCACCCCAGGGTTAACTATTATCAGAAGGGTACTATAAAGCGTTGTCGAACAAATCtacatac                          <  2:800316/100‑1 (MQ=255)
                                                                      aCAGTGATTTCATCCATATCTCCAGGACCGGGGCATCTCACCCCAGGGTTAACTATTATCAGAAGGGTACTATAAAGCGTTGTCGAACAAATCtacatac                          >  2:2304002/1‑100 (MQ=255)
                                                                       cAGTGATTTCATCCATATCTCCAGGACCGGGGCATCTCACCCCAGGGTTAACTATTATCAGAAGGGTACTATAAAGCGTTGTCGAACAAATCtacataca                         <  2:497349/100‑1 (MQ=255)
                                                                       cAGTGATTTCATCCATATCTCCAGGACCGGGGCATCTCACCCCAGGGTTAACTATTATCAGAAGGGTACTATAAAGCGTTGTCGAACAAATCtacataca                         >  1:2799786/1‑100 (MQ=255)
                                                                         gTGATTTCATCCATATCTCCAGGACCGGGGCATCTCACCCCAGGGTTAACTATTATCAGAAGGGTACTATAAAGCGTTGTCGAACAAATCTACATACAGa                       >  2:894877/1‑100 (MQ=255)
                                                                          cgATCTCATCCATATCTCCAGGACCGGGGCATCTCACCCCAGGGTTAACTATTATCAGAAGGGGACTATAAAGCGTTGTCGAACAAATCTACATACAGac                      <  1:2147208/99‑1 (MQ=255)
                                                                           gATTTCATCCATATCTCCAGGACCGGGGCATCTCACCCCAGGGTTAACTATTATCAGAAGGGTACTATAAAGCGTTGTCGAACAAATCTACATACAGaca                     <  2:2564911/100‑1 (MQ=255)
                                                                               tCATCCATATCTCCAGGACCGGGGCATCTCACCCCAGGGTTAACTATTATCAGAAGGGTACTATAAAGCGTTGTCGAACAAATCTACATACAGACACGcc                 >  1:942156/1‑100 (MQ=255)
                                                                                cATCCATATCTCCAGGACCGGGGCATCTCACCCCAGGGTTAACTATTATCAGAAGGGTACTATAAAGCGTTGTCGAACAAATCTACATACAGacagatcg                >  2:2147185/1‑95 (MQ=255)
                                                                                 aTCCATATCTCCAGGACCGGGGCATCTCACCCCAGGGTTAACTATTATCAGAAGGGTACTATAAAGCGTTGTCGAACAAATCTACATACAGACACGCCCt               <  1:2829449/100‑1 (MQ=255)
                                                                                  tCCATATCTCCAGGACCGGGGCATCTCACCCCAGGGTTAACTATTATCAGAAGGGTACTATAAAGCGTTGTCGAACAAATCTACATACAGACACGCCCta              <  1:1964187/100‑1 (MQ=255)
                                                                                   ccATATCTCCAGGACCGGGGCATCTCACCCCAGGGTTAACTATTATCAGAAGGGTACTATAAAGCGTTGTCGAACAAATCTACATACAGACACGCCCtat             <  1:1673878/100‑1 (MQ=255)
                                                                                   ccATATCTCCAGGACCGGGGCATCTCACCCCAGGGTTAACTATTATCAGAAGGGTACTATAAAGCGTTGTCGAACAAATCTACATACAGACACGCCCtat             <  2:2721790/100‑1 (MQ=255)
                                                                                   ccATATCTCCAGGACCGGGGCATCTCACCCCAGGGTTAACTATTATCAGAAGGGTACTATAAAGCGTTGTCGAACAAATCTACATACAGACACGCCCtat             <  2:1728437/100‑1 (MQ=255)
                                                                                    cATATCTCCAGGACCGGGGCATCTCACCTCAGGGTTAACTATTATCAGAAGGGTACTATAAAGCGTTGTCGAACAAATCTACATACAGACACGCCCtatt            <  1:122212/100‑1 (MQ=255)
                                                                                     atatCTCCAGGACCGGGGCATCTCACCCCAGGGTTAACTATTATCAGAAGGGTACTATAAAGCGTTGTCGAACAAATCTACATACAGACACGCCCtatta           >  1:2221106/1‑100 (MQ=255)
                                                                                     atatCTCCAGGACCGGGGCATCTCACCCCAGGGTTAACTATTATCAGAAGGGTACTATAAAGCGTTGTCGAACAAATCTACATACAGACACGCCCtatta           <  2:2903534/100‑1 (MQ=255)
                                                                                      tatCTCCAGGACCGGGGCATCTCACCCCAGGGTTAACTATTATCAGAAGGGTACTATAAAGCGTTGTCGAACAAATCTACATACAGACACGCCCtattat          >  1:1967287/1‑100 (MQ=255)
                                                                                        tctcCAGGACCGGTGCATCTCACCCCAGGGTTAACTATTATCAGAAGGGTACTATAAAGCGTTGTCGAACAAATCTACATACAGACACGCCCTATTatca        <  1:2911540/100‑1 (MQ=255)
                                                                                        tctcCAGGACCGGGGCATCTCACCCCAGGGTTAACTATTATCAGAAGGGTACTATAAAGCGTTGTCGAACAAATCTACATACAGACACGCCCTATTatca        <  1:2444392/100‑1 (MQ=255)
                                                                                        tctcCAGGACCGGGGCATCTCACCCCAGGGTTAACTATTATCAGAAGGGTACTATAAAGCGTTGTCGAACAAATCTACATACAGACACGCCCTATTatca        <  2:2287819/100‑1 (MQ=255)
                                                                                         ctcCAGGTCCGTGGTATCTCACCCCAGGGTTAACTATTATCAGAAGGGTACTATAAAGCGTTGTCGAACAAATCTACATACAGACACGCCCTATTatcat       <  1:274941/100‑1 (MQ=255)
                                                                                         ctcCAGGACCGGGGCATCTCACCCCAGGGTTAACTATTATCAGAAGGGTACTATAAAGCGTTGTCGAACAAATCTACATACAGACACGCCCTATTatcat       >  1:2517830/1‑100 (MQ=255)
                                                                                         ctcCAGGACCGGGGCATCTCACCCCAGGGTTAACTATTATCAGAAGGGTACTATAAAGCGTTGTCGAACAAATCTACATACAGACACGCCCTATTatcat       >  2:2865759/1‑100 (MQ=255)
                                                                                         ctcCAGGACCGGGGCATCTCACCCCAGGGTTAACTATTATCAGAAGGGTACTATAAAGCGTTGTCGAACAAATCTACATACAGACACGCCCTATTatcat       >  1:2709930/1‑100 (MQ=255)
                                                                                         ctcCAGGACCGGGGCATCTCACCCCAGGGTTAACTATTATCAGAAGAGTACTATAAAGCGTTGTCGAACAAATCTACATACAGACACGCCCTATTatcat       >  1:100564/1‑100 (MQ=255)
                                                                                          tcCAGGACCGGGGCATCTCACCCCAGGGTTAACTATTATCAGAAGGGTACTATAAAGCGTTGTCGAACAAATCTACATACAGACACGCCCTATTatcatc      >  2:711037/1‑100 (MQ=255)
                                                                                           ccAGGACCGGGGCATCTCACCCCAGGGTTAACTATTATCAGAAGGGTACTATAAAGCGTTGTCGAACAAATCTACATACAGACACGCCCTATTatcatc      <  2:2445936/99‑1 (MQ=255)
                                                                                           ccAGGACCGGGGCATCTCACCCCAGGGTTAACTATTATCAGAAGGGTACTATAAAGCGTTGTCGAACAAATCTACATACAGACACGCCCTATTATCATCt     >  2:2021117/1‑100 (MQ=255)
                                                                                           ccAGGACCGGGGCATCTCACCCCAGGGTTAACTATTATCAGAAGGGTACTATAAAGCGTTGTCGAACAAATCTACATACAGACACGCCCTATTATCATCt     >  2:1045751/1‑100 (MQ=255)
                                                                                              ggACCGGGGCATCTCACCCCAGGGTTAACTATTATCAGTAGGGTACTATAAAGCGTTGCCGAACAAATCTACATACAGACACGCCCTATTATCATCTAtt  <  2:1649711/100‑1 (MQ=255)
                                                                                              ggACCGGGGCATCTCACCCCAGGGTTAACTATTATCAGAAGGGTACTATAAAGCGTTGTCGAACAAATCTACATACAGACACGCCCTATTATCATCTAtt  >  2:1547171/1‑100 (MQ=255)
                                                                                              ggACCGGGGCATCTCACCCCAGGGTTAACTATTATCAGAAGGGTACTATAAAGCGTTGTCGAACAAATCTACATACAGACACGCCCTATTATCATCTAtt  >  1:2856586/1‑100 (MQ=255)
                                                                                                 |                                                                                                
GCTTCAGCAGCAAAAGTGATTGGTGCATGCAGGGCAACGGCCATTGTGGTCGCCAGCAGCGTTACTTTAAACAGTGATTTCATCCATATCTCCAGGATCGGGGCATCTCACCCCAGGGTTAACTATTATCAGAAGGGTACTATAAAGCGTTGTCGAACAAATCTACATACAGACACGCCCTATTATCATCTATT  >  NC_000913/3477337‑3477530

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: