Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,524,413 A→G 100% I515V (ATT→GTT)  mrcA → penicillin‑binding protein 1a, murein transglycosylase and transpeptidase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,524,4130AG97.2% 425.0 / ‑1.0 131I515V (ATT→GTT) mrcApenicillin‑binding protein 1a, murein transglycosylase and transpeptidase
Reads supporting (aligned to +/- strand):  ref base A (4/1);  new base G (59/67);  total (63/68)
Fisher's exact test for biased strand distribution p-value = 1.95e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 131 total aligned reads displayed.

GCAAGTATGTTGAACGATGTGCCAATTTCTCGCTGGGATGCAAGTGCCGGTTCTGACTGGCAGCCGAAGAACTCACCACCGCAGTATGCTGGTCCAATTCGCTTACGTCAGGGGCTGGGTCAGTCGAAAAACGTGGTGATGGTACGCGCAATGCGGGCGATGGGCGTCGACTACGCTGCAGAATATCTGCA  >  NC_000913/3524317‑3524507
                                                                                                |                                                                                              
gCAAGTATGTTGAACGATGTGCCAATTTCTCGCTGGGATGCAAGTGCCGGTTCTGACTGGCAGCCGAAGAACTCACCACCGCAGTATGCTGGTCCAGTTc                                                                                             <  2:799588/100‑1 (MQ=255)
gCAAGTATGTTGAACGATGTGCCAATTTCTCGCTGGGATGCAAGTGCCGGTTCTGACTGGCAGCCGAAGAACTCACCACCGCAGTATGCTGGTCCAGTTc                                                                                             <  1:861977/100‑1 (MQ=255)
   aGTATGTTGAACGATGTGCCAATTTCTCGCTGGGATGCAAGTGCCGGTTCTGACTGGCAGCCGAAGAACTCACCACCGCAGTATGCTGGTCCAGTTCGCt                                                                                          <  1:340815/100‑1 (MQ=255)
   aGTATGTTGAACGATGTGCCAATTTCTCGCTGGGATGCAAGTGCCGGTTCTGACTGGCAGCCGAAGAACTCACCACCGCAGTATGCTGGTCCAGTTCGCt                                                                                          <  1:343280/100‑1 (MQ=255)
       tGTTGAACGATGTGCCAATTTCTCGCTGGGATGCAAGTGCCGGTTCTGACTGGCAGCCGAAGAACTCACCACCGCAGTATGCTGGTCCAGTTCGCTTACg                                                                                      <  1:3039274/100‑1 (MQ=255)
       tGTTGAACGATGTGCCAATTTCTCGCTGGGATGCAAGTGCCGGTTCTGACTGGCAGCCGAAGAACTCACCACCGCAGTATGCTGGTCCAGTTCGCTTACg                                                                                      <  1:1950816/100‑1 (MQ=255)
       tGTTGAACGATGTGCCAATTTCTCGCTGGGATGCAAGTGCCGGTTCTGACTGGCAGCCGAAGAACTCACCACCGCAGTATGCTGGTCCAGTTCGCTTACg                                                                                      >  1:1817888/1‑100 (MQ=255)
       tGTTGAACGATGTGCCAATTTCTCGCTGGGATGCAAGTGCCGGTTCTGACTGGCAGCCGAAGAACTCACCACCGCAGTATGCTGGTCCAGTTCGCTTACg                                                                                      >  2:1727843/1‑100 (MQ=255)
       tGTTGAACGATGTGCCAATTTCTCGCTGGGACGCAGGTGCCGGTTCTGACTGGCAGCCGAAAAACTCGCCGCCACAATATGCTGGTCCAATTCGCTTACg                                                                                      >  1:62325/1‑100 (MQ=255)
        gTTGAACGATGTGCCAATTTCTCGCTGGGATGCAAGTGTCGGTTCTGACTGGCAGCCGAAGAACTCACCACCGCAGTATGCTGGACCAGTTCACTTAc                                                                                       >  1:573238/1‑98 (MQ=255)
        gTTGAACGATGTGCCAATTTCTCGCTGGGATGCAAGTGCCGGTTCTGACTGGCAGCCGAAGAACTCACCACCGCAGTATGCTGGTCCAGTTCGCTTACGt                                                                                     >  2:2547766/1‑100 (MQ=255)
          tGAACGATGTGCCAATTTCTCGCTGGGATTCAAGTGCCTGTTCTGACTGGCAGCCGAAGAACTCACCACCGCAGTATGCTGGTCCAGTTCGCTTACGTCa                                                                                   <  1:258821/100‑1 (MQ=255)
          tGAACGATGTGCCAATTTCTCGCTGGGATGCAAGTGCCGGTTCTGACTGGCAGCCGAAGAACTCACCACCGCAGTATGCTGGTCCAGTTCGCTTACGTCa                                                                                   <  1:257834/100‑1 (MQ=255)
            aaCGATGTGCCAATTTCTCGCTGGGATGCAAGTGCCGGTTCTGACTGGCAGCCGAAGAACTCACCACCGCAGTATGCTGGTCCAGTTCGCTTACGTCAgg                                                                                 <  1:2301695/100‑1 (MQ=255)
             aCGATGTGCCAATTTCTCGCTGGGATGCAAGTGCCGGTTCTGACTGGCAGCCGAAGAACTCACCACCGCAGTATGCTGGTCCAGTTCGCTTACGTCAggg                                                                                >  1:2393072/1‑100 (MQ=255)
             aCGATGTGCCAATTTCTCGCTGGGATGCAAGTGCCGGTTCTGACTGGCAGCCGAAGAACTCACCACCGCAGTATGCTGGTCCAGTTCGCTTACGTCAggg                                                                                <  2:2199117/100‑1 (MQ=255)
              cGATGTGCCAATTTCTCGCTGGGATGCAAGTGCCGGTTCTGACTGGCAGCCGAAGAACTCACCACCGCAGTATGCTGGTCCAGTTCGCTTACGTCAggg                                                                                <  2:1913621/99‑1 (MQ=255)
               gATGTGCCAATTTCTCGCTGGGATGCAAGTGCCGGTTCTGACTGGCAGCCGAAGAACTCACCACCGCAGTATGCTGGTCCAGTTCGCTTACGTCAGGGGc                                                                              >  2:53106/1‑100 (MQ=255)
                   tgCCAATTTCTCGCTGGGATGCAAGTGCCGGTTCTGACTGGCTGCCGAAGAACTCACCACCGCAGTATGCTGGTCCAGTTCGCTTACGTCAGGGGCTggg                                                                          <  1:880310/100‑1 (MQ=255)
                   tgCCAATTTCTCGCTGGGATGCAAGTGCCGGTTCTGACTGGCAGCCGAAGAACTCACCACCGCAGTATGCTGGTCCAGTTCGCTTACGTCAGGGGCTggg                                                                          >  1:652222/1‑100 (MQ=255)
                    gCCAATTTCTCGCTGGGATGCAAGTGCCGGTTCTGACTGGCAGCCGAAGAACTCACCACCGCAGTATGCTGGTCCAGTTCGCTTACGTCAGGGGCTGGGt                                                                         >  2:1827006/1‑100 (MQ=255)
                    gCCAATTTCTCGCTGGGATGCAAGTGCCGGTTCTGACTGGCAGCCGAAGAACTCACCACCGCAGTATGCTGGTCCAGTTCGCTTACGTCAGGGGCTGGGt                                                                         <  1:2587270/100‑1 (MQ=255)
                    gCCAATTTCTCGCTGGGATGCAAGTGCCGGTTCTGACTGGCAGCCGAAGAACTCACCACCGCAGTATGCTGGTCCAGTTCGCTTACGTCAGGGGCTGGGt                                                                         <  1:1536862/100‑1 (MQ=255)
                    gCCAATTTCTCGCTGGGATGCAAGGGCCGGTTCTGACTGGCAGCCGAAGAACTCACGACCGCAGTATGCTGGTCCAGTTCGCTTACGTCAGGGGCTGGGt                                                                         <  2:1565725/100‑1 (MQ=255)
                     ccAATTTCTCGCTGGGATGCAAGTGCCGGTTCTGACTGGCAGCTGAAGAAGTCACCACCGCAGTATGCTGGTCCAGTTCGCTTACGTCAGGGGCTGGGTc                                                                        <  1:510796/100‑1 (MQ=255)
                     ccAATTTCTCGCTGGGATGCAAGTGCCGGTTCTGACTGGCAGCCGAAGAACTCACCACCGCAGTATGCTGGTCCAGTTCGCTTACGTCAGGGGCTGGGTc                                                                        <  2:32040/100‑1 (MQ=255)
                     ccAATTTCTCGCTGGGATGCAAGTGCCGGTTCTGACTGGCAGCCGAAGAACTCACCACCGCAGTATGCTGGTCCAGTTCGCTTACGTCAGGGGCTGGGTc                                                                        <  2:1966887/100‑1 (MQ=255)
                      cAATTTCTCGCTGGGATGCAAGTGCCGGTTCTGACTGGCAGCCGAAGAACTCACCACCGCAGTATGCTGGTCCAGTTCGCTTACGTCAGGGGCTGGGTCa                                                                       >  1:2445950/1‑100 (MQ=255)
                       aaTTTCTCGCTGGGATGCAAGTGCCGGTTCTGACTGGCAGCCGAAGAACTCACCACCGCAGTATGCTGGTCCAGTTCGCTTACGTCAGGGGCTGGGTCAg                                                                      >  2:105547/1‑100 (MQ=255)
                         tttCTCGCTGGGATGCAAGTGGCGGTTCTCACTGGCAGCCGAAGACCTCACCACGGCAGTATGCTGGTCCAGTTCGCTTACGTCAGGGGCTGGGTCAGTc                                                                    <  2:1287279/100‑1 (MQ=255)
                         tttCTCGCTGGGATGCAAGTGCCGGTTCTGACTGGCAGCCGAAGAACTCACCACCGCAGTATGCTGGTCCAGTTCGCTTACGTCAGGGGCTGGGTCAGTc                                                                    <  2:1799439/100‑1 (MQ=255)
                          ttCTCGCTGGGATTCAAGTGCCGGTTCTGACTGGCAGCCGAAGAACTCACCACCGCAGTATGCTGGTCCAGTTCGCTTACGTCAGGGGCTGGGTCAGt                                                                     <  2:1010950/98‑1 (MQ=255)
                              cGCTGGGATGCAAGTGCCGGTTCTGACTGGCAGCCGAAGAACTCACCACCGCAGTATGCTGGTCCAGTTCGCTTACGTCAGGGGCTGGGTCAGTCGaaaa                                                               >  1:2091174/1‑100 (MQ=255)
                                 tGGGACGCAGGTGCCGGTTCTGACTGGCAGCCGAAAAACTCGCCGCCACAATATGCTGGTCCAATTCGCTTACGTCAGGGACTGGGCCAGTCGAAAAACg                                                            >  2:811372/1‑100 (MQ=255)
                                  gggATGCAAGTGCCGGTTCTGACTGGCAGCCGAAGAACTCACCACCGCAGTATGCTGGTCCAGTTCGCTTACGTCAGGGGCTGGGTCAGTCGAAAAACgt                                                           >  2:2572981/1‑100 (MQ=255)
                                  gggATGCAAGTGCCGGTTCTGACTGGCAGCCGAAGAACTCACCACCGCAGTATGCTGGTCCAGTTCGCTTACGTCAGGGGCTGGGTCAGTCGAAAAACgt                                                           >  1:1081684/1‑100 (MQ=255)
                                   ggATGCAAGTGCCGGTTCTGACTGGCAGCCGAAGAACTCACCACCGCAGTATGCTGGTCCAGTTCGCTTACGTCAGGGGCTGGGTCAGTCGAAAAACgtg                                                          <  1:105548/100‑1 (MQ=255)
                                   ggATGCAAGTGCCGGTTCTGACTGGCAGCCGAAGAACTCACCACCGCAGTATGCTGGTCCAGTTCGCTTACGTCAGGGGCTGGGTCAGTCGAAAAACgtg                                                          <  2:2173628/100‑1 (MQ=255)
                                    gATGCAAGTGCCGTTTCTGAGTGGCAGCCGAAGATCTCACCACCGCAGTATGCTGGGCCAGTTGGCTTATGTCATGGGCTGGGTAAGTGGAAAAACgtgg                                                         <  2:1396646/100‑1 (MQ=255)
                                    gATGCAAGTGCCGGTTCTGACTGGCAGCCGAAGAACTCACCACCGCAGTATGCTGGTCCAGTTCGCTTACGTCAGGGGCTGGGTCAGTCGAAAAACgtgg                                                         <  1:68657/100‑1 (MQ=255)
                                    gATGCAAGTGCCGGTCCTCAGTGGCAGCCGAACAACTCTCCACCGCAGTATGCTGGTCCAGTTCGCTTACGTCAGGGGCTGGTTAAGTCGAAAAACgtgg                                                         <  2:3029871/100‑1 (MQ=255)
                                     aTGCAAGTGCCGGTTCTGACTGGCAGCCGAAGAACTCACCACCGCAGTATGCTGGTCCAGTTCGCTTACGTCAGGGGCTGGGTCAGTCGAAAAACgtggt                                                        <  1:53106/100‑1 (MQ=255)
                                      tGCAATTGCCGGTTCTGACTGGCAGCCGAAGAACTCACCACCGCAGTATGCTGGTCCAGTTCGCTTACGTCAGGGGCTGGGTCAGTCGAAAAACgt                                                           >  2:1330778/1‑96 (MQ=255)
                                      tGCAAGGGCCGGTTCTGACTGGCAGCCGAAGAACTCACCACCGCAGTATGCTGGTCCAGTTCGCTTACGTCAGGGGCTGGGTCAGTCGAAAAACgtgg                                                         <  2:2021159/98‑1 (MQ=255)
                                      tGCAAATGACGGTTCTGACTGGCAGCCAAAGAACTCACCACCGCAGTATGCTGGTCCAGTTCGCTTACGTCAGGGGCTGGGTCAGTCGAAAAACgtggtg                                                       >  2:1924982/1‑100 (MQ=255)
                                       gCAAGTGCCGGTTCTGACTGGCAGCCGAAGAACTCACCACCGCAGTATGCTGGTCCAGTTCGCTTACGTCAGGGGCTGGGTCAGTCGAAAAACGTGGTGa                                                      >  1:2235010/1‑100 (MQ=255)
                                        cAAGTGCCGGTTCTGACTGGCAGCCGAAGAACTCACCACCGCAGTATGCTGGTCCAGTTAGCTTACGTCAGGGGCTGGGTCAGTCGAAAAACGTGGTGAt                                                     >  2:1810846/1‑100 (MQ=255)
                                          aGTGCCGGTTCTGACTGGCAGCCGAAGAACTCACCACCGCAGTATGCTGGTCCAGTTCGCTTACGTCAGGGGCTGGGTCAGTCGAAAAACGTGGTGATgg                                                   <  1:1145745/100‑1 (MQ=255)
                                          aGTGCCGGTTCTGACTGGCAGCCGAAGAACTCACCACCGCAGTATGCTGGTCCAGTTCGCTTACGTCAGGGGCTGGGTCAGTCGAAAAACGTGGTGATgg                                                   <  2:643627/100‑1 (MQ=255)
                                            tGCCGGTTCTGTCTGGCAGCCGAAGAACTCACCACCGCAGTATGCTGGTCCAGTTCGCTTACGTCAGGGGCTGGGTCAGTCGAAAAACGTGGTGATGGTa                                                 <  1:412176/100‑1 (MQ=255)
                                            tGCCGGTTCTGACTGGCAGCCGAAGAACTCACCACCGCAGTATGCTGGTCCAGTTCGCTTACGTCAGGGGCTGGGTCAGTCGAAAAACGTGGTGATGGTa                                                 >  2:2344441/1‑100 (MQ=255)
                                              ccGGTTCTGACTGACAGCCGAAGAACTCACCACCGCAGTATGCTGGTCCAGTTCGCTTACGTCAGGGGCTGGGTCAGTCGAAAAACGTGGTGATGGTAcg                                               >  1:2710766/1‑100 (MQ=255)
                                                ggTTCTGACTGGCAGCCGAAGAACTCACCACCGCAGTATGCTGGTCCAGTTCGCTTACGTCAGGGGCTGGGTCAGTCGAAAAACGTGGTGATGGTAcgcg                                             >  2:1426190/1‑100 (MQ=255)
                                                   tCTGACTGGCAGCCGAAGAACTCACCACCGCAGTATGCTGGTCCAGTTCGCTTACGTCAGGGGCTGGGTCAGTCGAAAAACGTGGTGATGGTACGCGCaa                                          >  2:1094641/1‑100 (MQ=255)
                                                    cTGACTGGCAGCCGAAGAACTCACCACCGCAGTATGCTGGTCCAGTTCGCTTACGTCAGGGGCTGGGTCAGTCGAAAAACGTGGTGATGGTACGCGCAAt                                         >  1:1168016/1‑100 (MQ=255)
                                                      gACTGGCAGCCGAAGAACTCACCACCGCAGTATGCTGGTCCAGTTCGCTTACGTCAGGGGCTGGGTCAGTCGACAAACGTGGTGATGGTACGCGCAATGc                                       <  1:1842466/100‑1 (MQ=255)
                                                      gACTGGCAGCCGAAGAACTCACCACCGCAGTATGCTGGTCCAGTTCGCTTACGTCAGGGGCTGGGTCAGTCGAAAAACGTGGTGATGGTACGCGCAATGc                                       >  1:588431/1‑100 (MQ=255)
                                                        cTGGCAGCCGAAGAACTCACCACCGCAGTATGCTGGTCCAGTTCGCTTACGTCAGGGGCTGGGTCAGTCGAAAAACGTGGTGATGGTACGCGCAATGCg                                      >  2:538435/1‑99 (MQ=255)
                                                         tcgCAGCCGAAGAACTCACCACCGCAGTATGCTGGTCCAGTTCGCTTACGTCAGGGGCTGGGTCAGTCGAAAAACGTGGTGATGGTACGCGCAATGCggg                                    <  2:321194/98‑1 (MQ=255)
                                                         tGGCCGCCGAAGAACTCACCACCGCAGTATGCTGGTCCAGTTCGCTTACGTCAGGGGCTGGGTCAGTCGAAAAACGTGGTGATGGTACGCGCAATGCggg                                    <  2:729194/100‑1 (MQ=255)
                                                          ggCATCCGAAGAACTCACCACCGCAGTATTCTTGTCCAGTTCTCTTACGTCAGGTGCTGGCTCAGTCGAAAAACGTGGTGATCGTACACGCAGTACggac                                   >  2:1624016/1‑98 (MQ=255)
                                                          ggCAGCCGAAGACCTCACCACCGCAGTATGCTGGTCCAGTTCGCTTACGTCAGGGGCTGGGTCAGTCGAATAACGTTGTGATGGTACGCGCAATGCGGGc                                   <  2:1921087/100‑1 (MQ=255)
                                                          ggCAGCCGAAGAACTCACCACCGCAGTATGCTGGTCCAGTTCGCTTACGTCAGGGGCTGGGTCAGTCGAAAAACGTGGTGATGGTACGCGCAATGCGGGc                                   >  2:2067036/1‑100 (MQ=255)
                                                          ggCAGCCGAAGAACTCACCACCGCAGTATGCTGGTCCAGTTCGCTTACGTCAGGGGCTGGGTCAGTCGAAAAACGTGGTGATGGTACGCGCAATGCGGGc                                   >  1:29686/1‑100 (MQ=255)
                                                            cAGCCGAAGAACTCACCACCGCAGTATGCTGGTCCAGTTCGCTTACGTCAGGGGCTGGGTCAGTCGAAAAACGTGGTGATGGTACGCGCAATGCGGGCGa                                 >  1:1286744/1‑100 (MQ=255)
                                                             aGCCGAAGAACTCACCACCGCAGTATGCTGGTCCAGTTCGCTTACGTCAGGGGCTGGGTCAGTAGCAAAACGTAGTGATGGTACGCGCAATGCGCGCGa                                 >  1:1491223/1‑99 (MQ=255)
                                                               ccGAAGAACTCACCACCGCAGTATTCTGGTCCAGTTCGCTTACGTCAGGGGCTGGGTAAGTCGAAAAACGTGGTGATGGTACGCGCAATGCGGGCGATgg                              <  1:1089201/100‑1 (MQ=255)
                                                               ccGAAGAACTCACCACCGCAGTATGCTGGTGCAATTCGCTTACGTCAGGGGCTGGGTCAGTCGAAAAACGTGGTGATGGTACGCGCAATACGGGCGATgg                              >  2:1997900/1‑100 (MQ=255)
                                                                cGAAGAACTCACCACCGCAGTATGCTGGTCCAGTTCGCTTACGTCAGGGGCTGGGTCAGTCGAAAAACGTGGTGATGGTACGCGCAATGCGGGCGATggg                             >  2:1731781/1‑100 (MQ=255)
                                                                cGAAGAACTCACCACCGCAGTATGCTGGTCCAGTTCGCTTACGTCAGGGGCTGGGTCAGTCGAAAAACGTGGTGATGGTACGCGCAATGCGGGCGATggg                             <  2:1259199/100‑1 (MQ=255)
                                                                cGAAGAACTCACCACCGCAGTATGCTGGTCCAGTTCGCTTACGTCAGGGGCTGGGTCAGTCGAAAAACGTGGTGATGGTACGCGCAATGCGGGCGATggg                             <  1:1150752/100‑1 (MQ=255)
                                                                cGAAGAACTCACCACCGCAGTATGCTGGTCCAGTTCGCTTACGTCAGGGGCTGGGTCAGTCGAAAAACGTGGTGATGGTACGCGCAATGCGGGCGATggg                             >  1:275815/1‑100 (MQ=255)
                                                                cGAAGAACTCACCACCGCAGTATGCTGGTCCAGTTCGCTTACGTCAGGGGCTGGGTCAGTCGAAAAACGTGGTGATGGTACGCGCAATGCGGGCGATggg                             >  1:1777819/1‑100 (MQ=255)
                                                                 gaagaaCTCACCACCGCAGTATGCTGGTCCAGTTCGCTTACGTCAGGGGCTGGGTCAGTCGAAAAACGTGGTGATGGTACGCGCAATGCGGGCGATGGGc                            >  2:2753812/1‑100 (MQ=255)
                                                                 gaagaaCTCACCACCGCAGTATGCTGGTCCAGTTCGCTTACGTCAGGGGCTGGGTCAGTCGAAAAACGTGGTGATGGTACGCGCAATGCGGGCGATGGGc                            <  1:2236003/100‑1 (MQ=255)
                                                                 gaagaaCTCACCACCGCAGTATGCTGGTCCAGTTCGCTTACGTCAGGGCCTGGGTCAGTCGAAAAACGTGGTGATGGTACGCGCAATGCGGGCGATGGGc                            >  2:1334196/1‑100 (MQ=255)
                                                                  aagtaCTTACCTCTGCATTATGCTGTTCTAGTTGGCTTACGTCAGGGGCTGGGTCAGTCGAAAAACGTGGTGATGGTACGCGCAATGCGGGCGATggg                             <  1:1465699/98‑1 (MQ=255)
                                                                  aagaaCTCACCACCGCAGTATGCTGGTCCAGTTCGCTTACGTCAGGGGCTGGGTCAGTCGAAAAACGTGGTGATGGTACGCGCAATGCGGGCGATGGGCg                           <  1:2011950/100‑1 (MQ=255)
                                                                      aCTCACCACCGCAGTATGCTGGTCCAGTTCGCTTACGTCAGGGGCTGGGTCAGTCGAAAAACGTGGTGATGGTACGCGCAATGCGGGCGATGGGCGTCGa                       >  2:2724464/1‑100 (MQ=255)
                                                                       cTCACCACCGCAGTATGCTGGTCCAGTTCGCTTACGTCAGGGGCTGGGTCAGTCGAAAAACGTGGTGATGGTACGCGCAATGCGGGCGATGGGCGTCGAc                      <  2:2257801/100‑1 (MQ=255)
                                                                       cTCACCACCGCAGTATGCTGGTCCAGTTCGCTTACGTCAGGGGCTGGGTCAGTCGAAAAACGTGGTGATGGTACGCGCAATGCGGGCGATGGGCGTCGAc                      <  2:1124845/100‑1 (MQ=255)
                                                                        tcgccacgGCAGTATGCTGATCCAGTTCGCTTACGTCAGTGTCGGGGTCAGTCTAAAAACGTGGTGATGTTACGCTCAATTCGGGCGATGGGCGTCGACt                     >  2:1004199/4‑100 (MQ=255)
                                                                        tCACCACCGCAGTATGCTGGTCCAGTTCGCTTACGTCAGGGGCTGGGTCAGTCGAAAAACGTGGTGATGGTACGCGCAATGCGGGCGATGGGCGTCGACt                     <  2:1537944/100‑1 (MQ=255)
                                                                         caccacCGCAGTATGCTGGTCCAGTTCGCTTACGTCAGGGGCTGGGTCAGTCGAAAAACGTGGTGATGGTACGCGCAATGCGGGCGATGGGCGTCGACTa                    >  2:178016/1‑100 (MQ=255)
                                                                         caccacCGCAGTATGCTGGTCCAGTTCGCTTACGTCAGGGGCTGGGTCAGTCGAAAAACGTGGTGATGGTACGCGCAATGCGGGCGATGGGCGTCGACTa                    >  1:1931564/1‑100 (MQ=255)
                                                                         caccacCGCAGTATGCTGGTCCAGTTCGCTTACGTCAGGGGCTGGGTCAGTCGAAAAACGTGGTGATGGTACGCGCAATGCGGGCGATGGGCGTCGACTa                    >  1:2929892/1‑100 (MQ=255)
                                                                          accaccGCAGTATGCTGGTCCAGTTCGCTTACGTCAGGGGTTGGGTCAGTCGAAAAACGTGGTGATGGTACGCGCAATGCGGGCGATGGGCGTCGACTAc                   <  1:2585756/100‑1 (MQ=255)
                                                                          accaccGCAGTATGCTGGTCCAGTTCGCTTACGTCAGGGGCTGGGTCAGTCGAAAAACGTGGTGATGGTACGCGCAATGCGGGCGATGGGCGTCGACTAc                   <  2:2796497/100‑1 (MQ=255)
                                                                           ccaccGCAGTATGCTGGTCCAGTTCGCTTACGTCAGGGGCTGGGTCAGTCGAAAAACGTGGTGATGGTACGCGCAATGCGGGCGATGGGCGTCGACTACg                  <  1:178018/100‑1 (MQ=255)
                                                                           ccaccGCAGTATGCTGGTCCAGTTCGCTTACGTCAGGGGCTGGGTCAGTCGAAAAACGTGGTGATGGTACGAGCAATGCGGGCGATGGGCGTCGACTACg                  >  1:397380/1‑100 (MQ=255)
                                                                            caccGCAGTATGCTGGTCCAGTTCGCTTACGTCAGGGGCTGGGTCAGTCGAAAAACGTGGTGATGGTACGCGCAATGCGGGCGATAGGCGTCGACTACGc                 >  1:1336272/1‑100 (MQ=255)
                                                                               cGCAGTATGCTGGTCCAGTTCGCTTACGTCAGGGGCTGGGTCAGTCGAAAAACGTGGTGATGGTACGCGCAATGCGGGCGATGGGCGTCGACTACGCTGc              <  1:1286025/100‑1 (MQ=255)
                                                                                 cAGTATGCTGGTCCAGTTCGCTTACGTCAGGGGCTGGGTCAGTCGAAAAACGTGGTGATGGTACGCGCAATGCGGGCGATGGGCGTCGACTACGCTGCAg            <  2:29686/100‑1 (MQ=255)
                                                                                  aGTATGCTGGTCCAGTTCGCTTACGTCAGGGGCTGGGTCAGTCGAAAGACGTGGTGATGGTACGCGCAATGCGGGCGATGGGCGTCGACTACGCTGCAGa           <  2:1470782/100‑1 (MQ=255)
                                                                                     aTGCTGGTGCAGGTCGGTTACGTGAGGGGTGGGGGCAGTCGAAAAACGTGGTGGTGGTACGCGTAATGCGGTCGATGGGCTTCGACTACGCTGCa             <  2:2756091/95‑1 (MQ=255)
                                                                                     aTGCTGGTCCAGTTCGCTTACGTCAGGGGCTGGGTCAGTCGAAAAACGTGGTGATGGTACGCGCAATGCGGGCGATGGGCGTCGACTACGCTGCAGAata        >  1:2760885/1‑100 (MQ=255)
                                                                                       gCTGGTCCAGTTCGCTTACGTCAGGGGCTGGGTCAGTCGAAAAACGTGGTGATGGTACGCGCAATGCGGGCGATGGGCGTCGACTACGCTGCAGAATATc      >  2:1190491/1‑100 (MQ=255)
                                                                                        cTGGTCCAGTTCGCTTACGTCAGGGGCTGGGTCAGTCGAAAAACGTGGTGATGGTACGCGCAATGCGGGCGATGGGCGTCGACTACGCTGCCGAATATc      >  2:2875727/1‑99 (MQ=255)
                                                                                         tGGTCCAGTTCGCTTACGTCAGGGGCTGGGTCAGTCGAAAAACGTGGTGATGGTACGCGCAATGCGGGCGATGGGCGTCGACTACGCTGCAGAATATCTg    <  2:275812/100‑1 (MQ=255)
                                                                                           gTCCAGTTCGCTTACGTCAGGGGCTGGGTCAGTCGAAAAACGTGGTGATGGTACGCGCAATGCGGGCGATGGGCGTCGACTACGCTGCAGAATATCTGCa  >  1:1101899/1‑100 (MQ=255)
                                                                                                |                                                                                              
GCAAGTATGTTGAACGATGTGCCAATTTCTCGCTGGGATGCAAGTGCCGGTTCTGACTGGCAGCCGAAGAACTCACCACCGCAGTATGCTGGTCCAATTCGCTTACGTCAGGGGCTGGGTCAGTCGAAAAACGTGGTGATGGTACGCGCAATGCGGGCGATGGGCGTCGACTACGCTGCAGAATATCTGCA  >  NC_000913/3524317‑3524507

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: