Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 252,594 G→C 15.4% W98C (TGG→TGC yafO → mRNA interferase toxin of the YafO‑YafN toxin‑antitoxin system

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913252,5940GC15.4% 278.6 / 39.2 104W98C (TGG→TGCyafOmRNA interferase toxin of the YafO‑YafN toxin‑antitoxin system
Reads supporting (aligned to +/- strand):  ref base G (42/45);  new base C (7/9);  total (50/54)
Fisher's exact test for biased strand distribution p-value = 7.91e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.85e-01

Only 100 of 107 total aligned reads displayed.

TGAGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACGAATGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGAACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCG  >  NC_000913/252495‑252692
                                                                                                   |                                                                                                  
tGAGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACGAATGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATgg                                                                                                    >  1:1552254/1‑100 (MQ=255)
 gagaATAATCCATTTCCGCCACAGTTGTGCCAATTCAGCAGAACGAATGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGt                                                                                                   <  2:664941/100‑1 (MQ=255)
 gagaATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACGAATGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGt                                                                                                   <  1:2796190/100‑1 (MQ=255)
   gaATAATTCATGGCCGCCACATGTGCTTCAAGGCAGCAGAACGAATGACGAATCGCATTTGTTATATGGTCAGGGGGCGTTTGATGAGCAAGCATGGTTg                                                                                                 <  1:2538140/100‑1 (MQ=255)
     ataatCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACGAATGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCt                                                                                               >  2:658451/1‑100 (MQ=255)
      ttatCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACGAATGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTc                                                                                              >  1:2057311/3‑100 (MQ=255)
      taatCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACGAATGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTc                                                                                              >  2:472484/1‑100 (MQ=255)
       aatCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACGAATGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCa                                                                                             <  2:571014/100‑1 (MQ=255)
       aatCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACGAATGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAACCATGCTTGCTCa                                                                                             <  2:325537/100‑1 (MQ=255)
       aatCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACGAATGAAGAAGCGCATTTGGTATATTATCAGGGAGCGTTTGATGAGCAAGAATGGTTGCTCa                                                                                             >  2:1156471/1‑100 (MQ=255)
        atCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACGAATGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCAt                                                                                            >  1:2858988/1‑100 (MQ=255)
        atCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACGAATGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCAt                                                                                            >  1:2390851/1‑100 (MQ=255)
        atCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACGAATGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAACCATGCTTGCTCAt                                                                                            <  2:1976367/100‑1 (MQ=255)
         tCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACGAATGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCAtt                                                                                           >  1:102276/1‑100 (MQ=255)
         tCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACGAATGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCAtt                                                                                           >  1:102440/1‑100 (MQ=255)
         tCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACGAATGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAACCATGCTTGCTCAtc                                                                                           <  1:1066501/100‑2 (MQ=255)
         tCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACGAATGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAACCATGCTTGCTCAtc                                                                                           <  2:2901334/100‑2 (MQ=255)
         tCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACGAATGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAACCATGCTTGCTCAtc                                                                                           <  1:1430103/100‑2 (MQ=255)
         tCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACGAATGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAACCATGCTTGCTCAtc                                                                                           <  1:2481304/100‑2 (MQ=255)
          ccATTTCCGCCACAGTTGCGCCAATTCAGCAGAACGAATGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTg                                                                                          <  1:2507857/100‑1 (MQ=255)
          ccATTTCCGCCACAGTTGCGCCAATTCAGCAGAACGAATGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAACCATGCTTGCTCAtca                                                                                          <  1:1122385/100‑3 (MQ=255)
          ccATTTCCGCCAAAGTTGCGCCAATTCAGCAGAACGAATGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAACCATGCTTGCTCAtc                                                                                           <  2:1072258/99‑2 (MQ=255)
           cATTTCCGCCACAGTTGCGTCAATTCAGCAGAACGAATGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGc                                                                                         <  1:2612136/100‑1 (MQ=255)
             tttCCGCCACAGTTGCGCCAATTCAGCAGAACGAATGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAACCATGCTTGCTCAtcaaac                                                                                       <  1:1284252/100‑6 (MQ=39)
               tCCGCCACAGTTGCGCCAATTCAGCAGAACGAATGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCAtt                                                                                     >  1:2358521/1‑100 (MQ=255)
                ccGCCACAGTTGCGCCAATTCAGCAGAACGAATGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTc                                                                                    >  1:147942/1‑100 (MQ=255)
                ccGCCACAGTTGCGCCAATTCAGCAGAACGAATGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTc                                                                                    <  2:2999502/100‑1 (MQ=255)
                 cGCCACAGTTGCGCCAATTCAGCAGAACGAATGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCt                                                                                   <  1:2238258/100‑1 (MQ=255)
                  gCCACAGTTGCGCCAATTCAGCAGAACGAATGACGAAGCGCATTTGGTATATTGTCAGGGAACGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTg                                                                                  >  1:757706/1‑100 (MQ=255)
                    cacaGTTGCGCCAATTCAGCAGAACGAATGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGaa                                                                                <  1:1039234/100‑1 (MQ=255)
                      caGTTGCGCCAATTCAGCAGAACGAATGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAAc                                                                              <  2:876782/100‑1 (MQ=255)
                          tGCGCCAATTCAGCAGAACGAATGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGa                                                                          <  2:1167439/100‑1 (MQ=255)
                          tGCGCCAATTCAGCAGAACGAATGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGa                                                                          >  2:1630867/1‑100 (MQ=255)
                            cgcCAATTCAGCAGAACGAATGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGaa                                                                         <  1:554372/99‑1 (MQ=255)
                            cgcCAATTCAGCAGAACGAATGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGaa                                                                         <  2:256801/99‑1 (MQ=255)
                             gcCAATTCAGCAGAACGAATGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGAAcc                                                                       >  2:1574454/1‑100 (MQ=255)
                              ccAATTCAGCAGAACGAATGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGAACCt                                                                      >  1:122435/1‑100 (MQ=255)
                                aaTTCAGCAGGACGAATGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGAACCTCa                                                                    <  2:540595/100‑1 (MQ=255)
                                aaTTCAGCAGAACGAATGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGAACCTCa                                                                    >  1:517253/1‑100 (MQ=255)
                                aaTTCAGCAGAACGAATGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGAACCTCa                                                                    <  1:1017521/100‑1 (MQ=255)
                                  ttCAACAGAAAGAATCAAGAAGAGGGGTTTGTATTTTGTCATAGGACAGTTGATGAGCTAGCATGGTTGCTCATTGTCATGCTGTAACCTGAACCtggta                                                                  >  2:1764182/1‑96 (MQ=255)
                                    cagcagAACGAATGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGAACCTCATaaa                                                                <  2:1520324/100‑1 (MQ=255)
                                    cagcagAACGAATGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGAACCTCATaaa                                                                >  1:2174333/1‑100 (MQ=255)
                                    cagcagAACGAATGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGAACCTCATaaa                                                                <  1:2203110/100‑1 (MQ=255)
                                      gcagAACGAATGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGAACCTCATAAACt                                                              <  1:1041286/100‑1 (MQ=255)
                                      gcagAACGAATGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGAACCTCATAAACt                                                              >  1:2539553/1‑100 (MQ=255)
                                      gcagAACGAATGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGAACCTCATAAACt                                                              <  1:2210710/100‑1 (MQ=255)
                                      cttccgatcttGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGAACCTCATAAACt                                                              <  1:1450817/90‑1 (MQ=255)
                                       cagAACGAATCACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGAACCTCATAAACTg                                                             <  2:1999374/100‑1 (MQ=255)
                                        agAACGAATGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGAACCTCATAAACTgg                                                            <  1:2554936/100‑1 (MQ=255)
                                         gAACGAATGACTAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGAACCTCATAAACTGGc                                                           <  1:1252427/100‑1 (MQ=255)
                                           aCGAATGACGAAGCGCATTTGGTATATTGTCTGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGAACCTCATAAACTGGCTc                                                         <  1:757881/100‑1 (MQ=255)
                                             gAATGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGAACCTCATAAACTGGCTCga                                                       <  1:2148745/100‑1 (MQ=255)
                                              aaTGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGAACCTCATAAACTGGCTCgag                                                      <  2:1044960/100‑1 (MQ=255)
                                              aaTGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGAACCTCATAAACTGGCTCgag                                                      >  1:1707547/1‑100 (MQ=255)
                                               aTGACGAAGCGCATTTGGTATATTGTCAGGGGGTGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGAACCTCATAAACTGGCTCgaga                                                     <  1:1048514/100‑1 (MQ=255)
                                               aTGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGAACCTCATAAACTGGCTCgaga                                                     <  1:53341/100‑1 (MQ=255)
                                               aTGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGAACCTCATAAACTGGCTCgaga                                                     <  2:1033365/100‑1 (MQ=255)
                                                tggcGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTTCTCATTGCCATTCTGAAACCTGAACCTCATAAACTGGCTCGAGAt                                                    <  2:1744394/97‑1 (MQ=255)
                                                tGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGAACCTCATAAACtagatcggaag                                                    >  2:1450803/1‑90 (MQ=255)
                                                tGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGATTGCTCATTGCCATTCTGAAACCTGAACCTCATAAACTGGCTCGAGAt                                                    >  1:225501/1‑100 (MQ=255)
                                                  atgttgcgcATTTGTTATATGGACAGGGGTCGTTAGATGGGCAAGCATGGTTGCTCGTTGCAATTGTGAAACGCGAACTTCATAAACTGGATCGAGAGaa                                                  <  2:808918/95‑1 (MQ=255)
                                                   cGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGAACCTCATAAACTGGCTCGAGATaac                                                 >  2:1389817/1‑100 (MQ=255)
                                                    gAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGAACCTCATAAACTGGCTCGAGATaaca                                                >  2:2281110/1‑100 (MQ=255)
                                                        cgcATTTGGTATATTGTCAGGTGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGAACCTCATAAACTGGCTCGAGATAACAACCa                                            <  2:2941050/100‑1 (MQ=255)
                                                        cgcATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGAACCTCATAAACTGGCTCGAGATAACAACCa                                            <  2:2390824/100‑1 (MQ=255)
                                                            tttGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGAACCTCATAAACTGGCTCGAGATAACAACCAAAtt                                        <  2:2358494/100‑2 (MQ=255)
                                                               ggTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGAACCTCATAAACTGGCTCGAGATAACAACCAAATGCAt                                     >  2:52179/1‑100 (MQ=255)
                                                                gTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGAACCTCATAAACTGGCTCGAGATAACAACCAAATGCATa                                    >  2:1133755/1‑100 (MQ=255)
                                                                gTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGAACCTCATAAACTGGCTCGAGATAACAACCAAATGCATa                                    >  1:1639997/1‑100 (MQ=255)
                                                                gTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAAACTGAAACTCATAAACTGGCTCGAGATAAAAACCAAATGCATa                                    >  1:1639775/1‑100 (MQ=255)
                                                                 tataTTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGAACCTCATAAACTGGCTCGAGATAACAACCAAATGCATaa                                   >  2:2562555/1‑100 (MQ=255)
                                                                      tGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGAACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTg                              <  2:1013712/100‑1 (MQ=255)
                                                                       gTCAGGGGGCGTTTGGTGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGAACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTgg                             >  2:2700464/1‑100 (MQ=255)
                                                                       gTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGAACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTgg                             <  2:603293/100‑1 (MQ=255)
                                                                        tCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGAACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTggg                            >  2:1817713/1‑100 (MQ=255)
                                                                         cAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGAACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGa                           >  2:2029432/1‑100 (MQ=255)
                                                                         cAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGAACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGa                           >  2:1850858/1‑100 (MQ=255)
                                                                          aGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGAACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGaa                          >  2:194810/1‑100 (MQ=255)
                                                                           gggggCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGAACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATAGGGaaa                         >  1:2582845/1‑100 (MQ=255)
                                                                             gggCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGAACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAAt                       >  1:2919644/1‑100 (MQ=255)
                                                                                cGTTTGGTGAGCAAGCATGGTTGCTAATTGCTGTTTTGAAAGCTGAGCCTCATAAACTGGCTCGAGATAACAAGGACATGCATAAAATTGGGAACATGGc                    >  2:2423091/1‑100 (MQ=255)
                                                                                cGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGAACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGc                    <  2:700709/100‑1 (MQ=255)
                                                                                cGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGAACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGc                    <  1:3027232/100‑1 (MQ=255)
                                                                                 gTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGAACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCa                   <  2:285658/100‑1 (MQ=255)
                                                                                   gcaatgaGCAACCATGCTTGCTCATTTCCATTCTGAAACCTGAACCTCATAAACTAGCACGAAATAACAACCAAATGCATAAAATTGGGAAAATGGCAGa                 >  1:804114/4‑100 (MQ=255)
                                                                                    caatgaGCAACCATGCTTGCTCATTGCCATTCTGAAACCTGAACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGaa                >  2:1714767/3‑100 (MQ=255)
                                                                                    caatgaGCAACCATGCTTGCTCATTGCCATTCTGAAACCTGAACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGaa                >  1:377765/3‑100 (MQ=255)
                                                                                    caatgaGCAACCATGCTTGCTCATTGCCATTCTGAAACCTGAACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGaa                >  2:742828/3‑100 (MQ=255)
                                                                                    caatgaGCAACCATGCTTGCTCATTGCCATTCTGAAACCTGAACCTCATAAACTGACTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGaa                >  1:944993/3‑100 (MQ=255)
                                                                                     gatgaGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGAACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAg               <  2:2071350/100‑1 (MQ=255)
                                                                                     gatgaGCAAGCATGGTGGCTCATTGCCATTCTGAAACCTGAACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAAGTGGGAAAATGGCGGAAg               >  2:2275359/1‑100 (MQ=255)
                                                                                      atgaGCAACCATGCTTGCTCATTGCCATTCTGAAACCTGAACCTCATAAACTGACTCGAAATAACAACCAAATGCATAAAATTGGGAAAATAGCAGAAg               >  1:2228284/1‑99 (MQ=255)
                                                                                       tgaGCAACCATGCTTGCTCATTGCCATTCTGAAACCTGAACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTAGGAAAATGGCAGAAGCg             >  2:1122424/1‑100 (MQ=255)
                                                                                         agcaagcaTGGTTGCTCATTGCCATTCTGAAACCTGAACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGtt           >  1:1718951/1‑100 (MQ=255)
                                                                                         agcaagcaTGGTTGCTCATTGCCATTCTGAAACCTGAACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGtt           <  1:1260801/100‑1 (MQ=255)
                                                                                            aagcaTGGTTGCTCATTGCCATTCTGAAACCTGAACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCg        >  1:2475792/1‑100 (MQ=255)
                                                                                                aTGGTTGCTCATTGCCATTCTGAAACCTGAACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATg    <  2:905747/100‑1 (MQ=255)
                                                                                                  ggTTGCTCATTGCCATTCTGAAACCTGAACCTCATAAACTGGCTCGAGATCACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCg  >  2:670485/1‑100 (MQ=255)
                                                                                                  ggTTGCTCATTGCCATTCTGAAACCTGAACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCg  >  2:687893/1‑100 (MQ=255)
                                                                                                   |                                                                                                  
TGAGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACGAATGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGAACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCG  >  NC_000913/252495‑252692

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: