BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	3,669,920	A→G	100%	E110G (GAA→GGA)	treF →	cytoplasmic trehalase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,669,920	0	A	G	98.3%	380.3 / ‑3.2	116	E110G (GAA→GGA)	treF	cytoplasmic trehalase
Reads supporting (aligned to +/- strand): ref base A (2/0); new base G (48/66); total (50/66)
Fisher's exact test for biased strand distribution p-value = 1.84e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.02e-01

Only 100 of 119 total aligned reads displayed.

TTCCCGACTGCGCACCTAAAATGGACCCGCTGGATATCTTAATCCGCTACCGTAAAGTGCGCCGTCATCGTGATTTTGACTTGCGCAAGTTTGTTGAAAACCACTTCTGGCTGCCGGAGGTCTACTCCAGCGAGTATGTATCGGACCCGCAAAATTCCCTGAAAGAGCATATCGACCAGCTGTGGCCGGTGCTAA  >  NC_000913/3669824‑3670018

  cccGACTGCGCACCTAAAATGGACCCGCTGGATATCTTAATCCGCTACCGTAAAGTGCGCCGTCATCGTGATTTTGACTTGCGCAAGTTTGTTGGAAAcc                                                                                               >  1:217929/1‑100 (MQ=255)

   ccGACTGTGCACCTAAAATGGACCCGCTGGATATCTTAATCCGCTACCGTAAAGTGCGCCGTCATCGTGATTTTGACTTGCGCAAGTTTGTTGGAAAcc                                                                                               <  2:1442935/99‑1 (MQ=255)

       cTGCGCACCTAAAATGGACCCGCTGGATATCTTAATCCGCTACCGTAAAGTGCGCCGTCATCGTGATTTTGACTTGCGCAAGTTTGTTGagatcggaaga                                                                                          >  2:2335020/1‑90 (MQ=255)

         gcgcACCTAAAATGGACCCGCTGGATATCTTAATCCGCTACCGTAAAGTGCGCCGTCATCGTGATTTTGACTTGCGCAAGTTTGTTGGAAACCACTTc                                                                                          >  1:1039305/1‑98 (MQ=255)

          cgcACCTAAAATGGACCCGCTGGATATCTTAATCCGCTCCCGTAAAGTGCGCCGTCATCGTGATTTTGACTTGCGCAAGTTTGTTGGAAACAACTTCTgg                                                                                       >  1:2650603/1‑100 (MQ=255)

          cgcACCTAAAATGGACCCGCTGGATATCTTAATCCGCTACCGTAAAGTGCGCCGTCATCGTGATTTTGACTTGCGCAAGTTTGTTGGAAACCACTTCTgg                                                                                       >  2:776190/1‑100 (MQ=255)

          cgcACCTAAAATGGACCCGCTGGATATCTTAATCCGCTACCGTAAAGTGCGCCGTCATCGTGATTTTGACTTGCGCAAGTTTGTTGGAAACCACTTCTgg                                                                                       <  2:208394/100‑1 (MQ=255)

           gcACCTAAAATGGACCCGCTGGATATCTTAATCCGCTACCGTAAAGTGCGCCGTCATCGTGATTTTGACTTGCGCAAGTTTGTTGGAAACCACTTCTGGc                                                                                      <  2:1205135/100‑1 (MQ=255)

           gcACCTAAAATGGACCCGCTGGATATCTTAATCCGCTACCGTAAAGTGCGCCGTCATCGTGATTTTGACTTGCGCAAGTTTGTTGGAAACCACTTCTGGc                                                                                      <  2:2410512/100‑1 (MQ=255)

            cACCTAAAATGGACCCGCTGGATATCTTAATCCGCTACCGTAAAGTGCGCCGTCATCGTGATTTTGACTTGCGCAAGTTTGTTGGAAACCACTTCTGGCt                                                                                     >  1:1267610/1‑100 (MQ=255)

             aCCTAAAATGGACCCGCTGGATATCTTAATCCGCTACCGTAAAGTGCGCCGTCATCGTGATTTTGACTTGCGCAAGTTTGTTGGAAACCACTTCTGGCTg                                                                                    <  1:1677602/100‑1 (MQ=255)

              ccTAAAATGGACCCGCTGGATATCTTAATCCGCTCCCGTAAAGTGCGCCGTCATCGTGATTTTGACTTGCGCAAGTTTGTTGGAAACCACTTCTGGc                                                                                      >  2:1833136/1‑97 (MQ=255)

               cTAAAATGGACCCGCTGGATATCTTAATCCGCTACCGTAAAGTGCGCCGTCATCGTGATTTTGACTTGCGCAAGTTTGTTGGAAACCACTTCTGGCTGcc                                                                                  <  2:690967/100‑1 (MQ=255)

                tAAAATGGACCCGCTGGATATCTTAATCCGCTACCGTAAAGTGCGCCGTCATCGTGATTTTGACTTGCGCAAGTTTGTTGGAAACCACTTCTGGCTGCCg                                                                                 >  2:2596600/1‑100 (MQ=255)

                tAAAATGGACCCGCTGGATATCTTAATCCGCTACCGTAAAGTGCGCCGTCATCGTGATTTTGACTTGCGCAAGTTTGTTGGAAACCACTTCTGGCTGCCg                                                                                 >  2:1020231/1‑100 (MQ=255)

                    aTGGACCCGCTGGATATCTTAATCCGCTACCGTAAAGTGCGCCGTCATCGTGATTTTGACTTGCGCAAGTTTGTTGGAAACCACTTCTGGCTGCCGGAgg                                                                             <  2:812756/100‑1 (MQ=255)

                     tgtaCCCGCTGTATATCTTAATCCGCTACCTTAAATTGCGCCGTTATCGTTATTTTGACTTGCGCAAGTTTGTTTGAAACCACTTCTGGCTGCCGGAgg                                                                             <  2:2930737/96‑1 (MQ=255)

                     tGGATCCGTTGGATATCTTAATCCGCTACCGTAAAGTGCGCCGTCATCGTGATTTTGACTTGCGCAAGTTTGTTGGAAACCACTTCTGGCTGCCGGAgg                                                                             <  1:593066/99‑1 (MQ=255)

                       gACCCGCTGGATATCTTAAGCCGCTACCGTAAAGTGCGCCGTCATCGTGATTTTGACTTGCGCAAGTTTGTTGGAAACCACTTCTGGCTGCCGGAgg                                                                             <  2:1101016/97‑1 (MQ=255)

                         cccGCTGGATATCTTAATCCGCTACCGTAAAGTGCGCCGTCATCGTGATTTTGACTTGCGCAAGTTTGTTGGAAACCACTTCTGGCTGCCGGAGGTCTAc                                                                        <  2:136474/100‑1 (MQ=255)

                          ccGCTGGATATCTTAATCCGCTACCGTAAAGTGCGCCGTCATCGTGATTTTGACTTGCGCAAGTTTGTTGGAAACCACTTCTGGCTGCCGGAGGTCTACt                                                                       <  1:2596632/100‑1 (MQ=255)

                           cGCTGGATATCTTAATCCGCTACCGTAAAGTGCGCCGTCATCGTGATTTTGACTTGCGCAAGTTTGTTGGAAACCACTTCTGGCTGCCGGAGGTCTACTc                                                                      >  1:1491511/1‑100 (MQ=255)

                           cGCTGGATATCTTAATCCGCTACCGTAAAGTGCGCCGTCATCGTGATTTTGACTTGCGCAAGTTTGTTGGAAACCACTTCTGGCTGCCGGAGGTCTACTc                                                                      >  1:779762/1‑100 (MQ=255)

                              tGGATATCTTAATCCGCTACCGTAAAGTGCGCCGTCATCGTGATTTTGACTTGCGCAAGTTTGTTGGAAACCACTTCTGGCTGCCGGAGGTCTACTCCAg                                                                   <  1:1020238/100‑1 (MQ=255)

                              tGGATATCTTAATCCGCTACCGTAAAGTGCGCCGTCATCGTGATTTTGACTTGCGCAAGTTTGTTGGAAACCACTTCTGGCTGCCGGAGGTCTACTCCAg                                                                   <  1:2345449/100‑1 (MQ=255)

                                gATATCTTAATCCGCTACCGTAAAGTGCGCTGTCATCGTGATTTTGACTTGCGCAAGTTTGTTGGAAACCACTTCTGGCTGCCGGAGGTCTACTCCAGCg                                                                 <  1:981354/100‑1 (MQ=255)

                                gATATCTTAATCCGCTACCGTAAAGTGCGCCGTCATCGTGATTTTGACTTGCGCAAGTTTGTTGGAAACCACTTCTGGCTGCCGGAGGTCTACTCCAGCg                                                                 <  1:2595088/100‑1 (MQ=255)

                                 atatCTTAATCCGCTACCGTAAAGTGCGCCGTCATCGTGATTTTGACTTGCGCAAGTTTGTTGGAAACCACTTCTGGCTGCCGGAGGTCTACTCCAGCg                                                                 <  2:104606/99‑1 (MQ=255)

                                  tatCTTAATCCGCTACCGTAAAGTGCGCCGTCATCGTGATTTTGACTTGCGCAAGTTTGTTGGAAACCACTTCTGGCTGCCGGAGGTCTACTCCAGCGAg                                                               <  2:1484161/100‑1 (MQ=255)

                                  tatCTTAATCCGCTACCGTAAAGTGCGCCGTCATCGTGATTTTGACTTGCGCAAGTTTGTTGGAAACCACTTCTGGCTGCCGGAGGTCTACTCCAGCGAg                                                               >  1:2658161/1‑100 (MQ=255)

                                    tCTTAATCCGCTACCGTAAAGTGTGCCGTCATCGTGATTTTGACTTGCGCAAGTTTGTTGGAAACCACTTCTGGCTGCCGGAGGTCTACTCCAGCGAgt                                                              <  2:1662080/99‑1 (MQ=255)

                                    tCTTAATCCGCTACCGTAAAGTGCGCCGTCATCGTGATTTTTACTTGCGCAAGTTTATTGGAAACCACTTCTGGCTGCCGGAGGTCTACTCCAGCGAgta                                                             >  1:207965/1‑100 (MQ=255)

                                    tCTTAATCCGCTACCGTAAAGTGCGCCGTCATCGTGATTTTGACTTGCGCAAGTTTGTTGGAAACCACTTCTGGCTGCCGGAGGTCTACTCCAGCGAgta                                                             >  1:1159813/1‑100 (MQ=255)

                                    tCTTAATCCGCTACCGTAAAGTGCGCCGTCATCGTGATTTTGACTTGCGCAAGTTTGTTGGAAACCACTTCTGGCTGCCGGAGGTCTACTCCAGCGAgta                                                             <  2:2343166/100‑1 (MQ=255)

                                     cTTAATCCGCTACCGTAAAGTGCGCCGTCATCGTGATTTTGACTTGCGCAAGTTTGTTGGAAACCACTTCTGGCTGCCGGAGGTCTACTCCAGCGAgtat                                                            >  2:688022/1‑100 (MQ=255)

                                     cTTAATCCGCTACCGTAAAGTGCGCCGTCATCGTGATTTTGACTTGCGCAAGTTTGTTGGAAACCACTTCTGGCTGCCGGAGGTCTACTCCAGCGAgtat                                                            >  2:2212756/1‑100 (MQ=255)

                                      ttAATCCGCTACCGTAAAGTGCGCCGTCATCGTGATTTTGACTTGCGCAAGTTTGTTGGAAACCACTTCTGGCTGCCGGAGGTCTACTCCAGCGAgtatg                                                           <  2:18621/100‑1 (MQ=255)

                                      ttAATCCACTACCGTAAAGTGCGCCGTCATCGTGATTTTGACTTGCGCAAGTTTGTTGGAAACCACTTCTGGCTGCCGGAGGTCTACTCCAGCGAgtatg                                                           >  1:2495768/1‑100 (MQ=255)

                                       tAATCCGCTACCGTAAAGTGCGCCGTCATCGTGATTTTGACTTGCGCAAGTTTGTTGGAAACCACTTCTGGCTGCCGGAGGTCTACTCCAGCGAgtatgt                                                          >  1:1739014/1‑100 (MQ=255)

                                       tAATCCGCTACCGTAAAGTGCGCCGTCATCGTGATTTTGACTTGCGCAAGTTTGTTGGAAACCACTTCTGGCTGCCGGAGGTCTACTCCAGCGAgtatat                                                          >  2:1835134/1‑98 (MQ=255)

                                         aTCCGCTACCGTAAAGTGCGCCGTCATCGTGATTTTGACTTGCGCAAGTTTGTTGGAAACCACTTCTGGCTGCCGGAGGTCTACTCCAGCGAgtatgtat                                                        >  2:391452/1‑100 (MQ=255)

                                         aTCCGCTACCGTAAAGTGCGCCGTCATCGTGATTTTGACTTGCGCAAGTTTGTTGGAAACCACTTCTGGCTGCCGGAGGTCTACTCCAGCGAgtatgtat                                                        >  2:1037729/1‑100 (MQ=255)

                                         aTCCGCTACCGTAAAGTGCGCCGTCATCGTGATTTTGACTTGCGCAAGTTTGTTGGAAACCACTTCTGGCTGCCGGAGGTCTACTCCAGCGAgtatgtat                                                        <  1:93464/100‑1 (MQ=255)

                                            cGCTACCGTAAAGTGCGCCGTCATCGTGATTTTGACTTGCGCAAGTTTGTTGGAAACCACTTCTGGCTGCCGGAGGTCTACTCCAGCGAGTATGTATCgg                                                     >  2:2316134/1‑100 (MQ=255)

                                               tACCGTAAAGTGCGCCGTCATCGTGATTTTGACTTGCGCAAGTTTGTTGGAAACCACTTCTGGCTGCCGGAGGTCTACTCCAGCGAGTATGTATCGGAcc                                                  <  1:3035472/100‑1 (MQ=255)

                                                aCCGTAAAGTGCGCCGTCATCGTGATTTTGACTTGCGCAAGTTTGTTGGAAACCACTTCTGGCTGCCGGAGGTCTACTCCAGCGAGTATGTATCGGAccc                                                 >  1:633544/1‑100 (MQ=255)

                                                aCCGTAAAGTGCGCCGTCATCGTGATTTTGACTTGCGCAAGTTTGTTGGAAACCACTTCTGGCTGCCGGAGGTCTACTCCAGCGAGTATGTATCGGAccc                                                 >  1:1672218/1‑100 (MQ=255)

                                                 ccGTAAAGTGTGCCGTCATCGTGATTTTGACTTGCGCAAGTTTGTTGGAAACCACTTCTGGCTGCCAGAGGTCTACTCCAGCGAGTATGTATCGGACCCg                                                <  2:1987272/100‑1 (MQ=255)

                                                         tGCGCCGTCATCGTGATTTTGACTTGCGCAAGTTTGTTGGAAACTACTTCTGGCTGCCGGAGGTCTACTCCAGCGAGTATGTATCGGACCCGCAAAATTc                                        <  1:1575002/100‑1 (MQ=255)

                                                         tGCGCCGTCATCGTGATTTTGACTTGCGCAAGTTTGTTGGAAACCACTTCTGGCTGCCGGAGGTCTACTCCAGCGAGTATGTATCGGACCCGCAAAATTc                                        <  1:1896604/100‑1 (MQ=255)

                                                          gcgcCGTCATCGTGATTTTGACTTGCGCAAGTTTGTTGGAAACCACTTCTGGCTGCCGGAGGTCTACTCCAGCGAGTATGTATCGGACCCGCAAAATTcc                                       <  2:2121260/100‑1 (MQ=255)

                                                           cgtgggcATTGTGATTTTGTTTTTCGCAATTTTGTTGGAAACTACTTCTTTCTTCTTGATGTCTACGCCAGCGAGTATGTATCGGACCCGCAAAATTccc                                      <  1:1764297/94‑1 (MQ=255)

                                                           cgcCGTCATCGTGATTTTGACTTGCGCAAGTTTGTTGGAAACCACTTCTGGCTGCCGGAGGTCTACTCCAGCGAGTATGTATCGGACCCGCAAAATTccc                                      <  1:1930638/100‑1 (MQ=255)

                                                            gcggtcggcGTGATTTTGACTTGCGCAAGTTTGGTGGAAACCACTTCTGGCTGCGGGAGGTCTACTCCAGCGAGTATGTATCGGACCCGCAAAATTCCCt                                     <  2:1868151/92‑1 (MQ=255)

                                                              cGTCATCGTGATTTTGACTTGCGCAAGTTTGTTGGAAACCACTTCTGGCTGCCGGAGGTCTACTCCAGCGAGTATGTATCGGACCCGCAAAATTCACTGa                                   >  1:1505903/1‑100 (MQ=255)

                                                               gTCATCGTGATTTTGACTTGCGCAAGTTTGTTGGAAACCACTTCTGGCTGCCGGAGGTCTACTCCAGCGAGTATGTATCGGACCCGCAAAATTCCCTg                                    <  2:825002/98‑1 (MQ=255)

                                                                 cATCGTGATTTTGACTTGCGCAAGTTTGTTGGAAATCACTTCTGGCTGCCGGAGGTCTACTCCAGCGAGTATGTATCGGACCCGCAAAATTCCCTGAAag                                <  1:2638639/100‑1 (MQ=255)

                                                                 cATCGTGATTTTGACTTGCGCAAGTTTGTTGGAAACCACTTCTGGCTGCCGGAGGTCTACTCCAGCGAGTATGTATCGGACCCGCAAAATTCCCTGAAag                                <  1:1483010/100‑1 (MQ=255)

                                                                   tCGTGATTTTGACTTGCGCAAGTTTGTTGGAAACCACTTCTGGCTGCCGGAGGTCTACTCCAGCGAGTATGTATCGGACCCGCAAAATTCCCTGAAagag                              >  1:516831/1‑100 (MQ=255)

                                                                   tCGTGATTTTGACTTGCGCAAGTTTGTTGGAAACCACTTCTGGCTGCCGGAGGTCTACTCCAGCGAGTATGTATCGGACCCGCAAAATTCCCTGAAagag                              <  2:2201383/100‑1 (MQ=255)

                                                                   tCGTGATTTTGACTTGCGCAAGTTTGTTGGAAACCACTTCTGGCTGCCGGAGGTCTACTCCAGCGAGTATGTATCGGACCCGCAAAATTCCCTGAAagag                              <  1:2393931/100‑1 (MQ=255)

                                                                    cGTGATTTTGACTTGCGCAAGTTTGTTGGAAACCACTTCTGGCTGCCGGAGGTCTACTCCAGCGAGTATGTATCGGACCCGCAAAATTCCCTGAAAGAGc                             <  1:1884559/100‑1 (MQ=255)

                                                                     gTGATTTTGACTTGCGCAAGTTTGTTGGAAACCACTTCTGGCTGCCGGAGGTCTACTCCAGCGAGTATGTATCGGACCCGCAAAATTCCCTGAAAGAGCa                            <  1:3020966/100‑1 (MQ=255)

                                                                     gTGATTTTGACTTGCGCAAGTTTGTTGGAAACCACTTCTGGCTGCCGGAGGTCTACTCCAGCGAGTATGTATCGGACCCGCAAAATTCCCTGAAAGAGCa                            <  1:1919154/100‑1 (MQ=255)

                                                                      tGATTTTGACTTGCGCAAGTTTGTTGGAAACCACTTCTGGCTGCCGGAGGTCTACTCCAGCGAGTATGTATCGGACCCGCAAAATTCCCTGAAAGAGCat                           >  2:2801646/1‑100 (MQ=255)

                                                                       gATTTTGACTTGCGCAAGTTTGTTGGAAACCACTTCTGGCTGCCGGAGGTCTACTCCAGCGAGTATGTATCGGACCCGCAAAATTCCCTGAAAGAGCata                          <  1:2786170/100‑1 (MQ=255)

                                                                       gATTTTGACTTGCGCAAGTTTGTTGGAAACCACTTCTGGCTGCCGGAGGTCTACTCCAGCGAGTATGTATCGGACCCGCAAAATTCCCTGAAAGAGCata                          >  2:1523554/1‑100 (MQ=255)

                                                                       gATTTTGACTTGCGCAAGTTTGTTGGAAACCACTTCTGGCTGCCGGAGGTCTACTCCAGCGAGTATGTATCGGACCCGCAAAATTCCCTGAAAGAGCata                          >  2:1058853/1‑100 (MQ=255)

                                                                        aTTTTGACTTGCGCAAGTTTGTTGGAAACCACTTCTGGCTGCCGGAGGTCTACTCCAGCGAGTATGTATCGGACCCGCAAAATTCCCTGAAAGAGCatat                         <  2:1324061/100‑1 (MQ=255)

                                                                           ttGACTTGCGCAAGTTTGTTGGAAACCACTTCTGGCTGCCGGAGGTCTACTCCAGCGAGTATGTATCGGACCCGCAAAATTCCCTGAAAGAGCATATc                        >  1:2927805/1‑98 (MQ=255)

                                                                             gACTTGCGCAAGTTTGTTGGAAACCACTTCTGGCTGCCGGAGGTCTACTCCAGCGAGTATGTATCGGACCCGCAAAATTCCCTGAAAGAGCATATCGAcc                    >  2:1690178/1‑100 (MQ=255)

                                                                               nttGCGCAAGTTTGTTGGAAACCACTTCTGGCTGCCGGAGGTCTACTCCAGCGAGTATGTATCGGACCCGCAAAATTCCCTGAAAGAGCATATCGACCAg                  >  2:111518/2‑100 (MQ=255)

                                                                               cTTTCGCAAGTTTGTTGGAAACCACTTCTGGCTGCCGGAGGTCTACTCCAGCGAGTATGTATCGGACCCGCAAAATTCCCTGAAAGAGCATATCGACCAg                  <  1:2007301/100‑1 (MQ=255)

                                                                               cTTGCGCAAGTTTGTTGGAAACCACTTCTGGCTGCCGGAGGTCTACTCCAGCGAGTATGTATCGGACCCGCAAAATTCCCTGAAAGAGCATATCGACCAg                  <  1:2479716/100‑1 (MQ=255)

                                                                               cTTGCGCAAGTTTGTTGGAAACCACTTCTGGCTGCCGGAGGTCTACTCCAGCGAGTATGTATCGGACCCGCAAAATTCCCTGAAAGAGCATATCGACCAg                  >  1:1799531/1‑100 (MQ=255)

                                                                               cTTGCGCAAGTTTGTTGGAAACCACTTCTGGCTGCCGGAGGTCTACTCCAGCGAGTATGTATCGGACCCGCAAAATTCCCTGAAAGAGCATATCGACCAg                  <  2:516828/100‑1 (MQ=255)

                                                                               cTTGCGCAAGTTTGTTGGAAACCACTTCTGGCTGCCGGAGGTCTACTCCAGCGAGTATGTATCGGACCCGCAAAATTCCCTGAAAGAGCATATCGACCAg                  >  1:1716448/1‑100 (MQ=255)

                                                                               cTTGCGCAAGTTTGTTGGAAACCACTTCTGGCTGCCGGAGGTCTACTCCAGCGAGTATGTATCGGACCCGCAAAATTCCCTGAAAGAGCATATCGACCAg                  <  2:507414/100‑1 (MQ=255)

                                                                                  gcgcAAGTTTGTTGGAAACCACTTCTGGCTGCCGGAGGTCTACTCCAGCGAGTATGTATCGGACCCGCAAAATTCCCTGAAAGAGCATATCGACCAGCtg               >  1:2768011/1‑100 (MQ=255)

                                                                                    gcAAGTTTGTTGGAAACCACTTCTGGCTGCCGGAGGTCTACTCCAGCGAGTATGTATCGGACCCGCAAAATTCCCTGAAAGAGCATATCGACCAGCtgtg             >  1:2462526/1‑100 (MQ=255)

                                                                                    gcAAGTTTGTTGGAAACCACTTCTGGCTGCCGGAGGTCTACTCCAGCGAGTATGTATCGGACCCGCAAAATTCCCTGAAAGAGCATATCGACCAGCtgtg             >  1:1102744/1‑100 (MQ=255)

                                                                                     cAAGTTTGTTGGAAACCACTTCTGGCTGCCGAACGTCTACTCCAGCTAGTATGTATCGGACCCTCAAAATTTCATAAAAGAGCATATCGATCAGCtg               <  2:2170889/97‑1 (MQ=255)

                                                                                      aaGTTTGTTGGAATCCACTTCTGGCTGCCGGAGGTCTACTCCAGCGAGTATGTATCGGACCCGCAAAATTCCCTGAAAGAGCATATCGACCAGCTGTGGc           <  1:2951941/100‑1 (MQ=255)

                                                                                      aaGTTTGTTGGAAACCACTTCTGGCTGCCGGAGGTCTACTCCAGCGAGTATGTATCGGACCCGCAAAATTCCCTGAAAGAGCATATCGACCAGCTGTGGc           <  1:963798/100‑1 (MQ=255)

                                                                                       attttGTTGGAAACCACTTCTGCCTGCCGGATGTCTACTCAAGCGAGTATCTATCGGACCCGCAAAATTCCCTTAAATAGCATATCGACCATCTGTGGcc          <  2:1191151/98‑1 (MQ=255)

                                                                                       aGTTTGTTGGAAACCACTTCTGGCTGCCGGAGGTCTACTCCAGCGAGTATGTATCGGACCCGCAAAATTCCCTTAAAGAGCATATCGACCAGCTGTGGcc          <  2:1055799/100‑1 (MQ=255)

                                                                                       aGTTTGTTGGAAACCACTTCTGGCTGCCGGAGGTCTACTCCAGCGAGTATGTATCGGACCCGCAAAATTCCCTGAAAGAGCATATCGACCAGCTGTGGcc          <  2:1737666/100‑1 (MQ=255)

                                                                                        gTTTGTTGGAAACCACTTCTGGCTGCCGGAGGTCTACTCCAGCGAGTATGTATCGGACCCGCAAAATTCCCTGAAAGAGCATATCGACCAGCTGTgg            >  2:1813552/1‑97 (MQ=255)

                                                                                        gTTTGTTGGAAACCACTTCTGGCTGCCGGAGGTCTACTCCAGCGAGTATGTATCGGACCCGCAAAATTCCCTGAAAGAGCATATCGACCAGCTGTGGCCg         >  2:905852/1‑100 (MQ=255)

                                                                                        gTTTGTTGGAAACCACTTCTGGCTGCCGGAGGTCTACTCCAGCGAGTATGTATCGGACCCGCAAAATTCCCTGAAAGAGCATATCGACCAGCTGTGGCCg         <  2:147561/100‑1 (MQ=255)

                                                                                         tttgtttgAAACCACTTCTGGCTGCCGGAGGTCTACTCCAGCGAGTATGTATCGGACCCGCAAAATTCCCTGAAAGAGCATATCGACCAGCTGTGGCCgg        <  1:1370240/100‑1 (MQ=255)

                                                                                           tgttgGAAACCACTTCTGGCTGCCGGAGGTCTACTCCAGCGAGTATGTATCGGACCCGCAAAATTCCCTGAAAGAGCATATCGACCAGCTGTGGCCGGTg      <  2:2323016/100‑1 (MQ=255)

                                                                                           tgttgGAAACCACTTCTGGCTGCCGGAGGTCTACTCCAGCGAGTATGTATCGGACCCGCAAAATTCCCTGAAAGAGCATATCGACCAGCTGTGGCCGGTg      <  1:776194/100‑1 (MQ=255)

                                                                                           tgttgGAAACCACTTCTGGCTGCCGGAGGTCTACTCCAGCGAGTATGTATCGGACCCGCAAAATTCCCTGAAAGAGCATATCGACCAGCTGTGGCCGGTg      >  1:2257897/1‑100 (MQ=255)

                                                                                           tgttgAAAACCACTTCTGGCTGCCGGAGGTCTACTCCAGCGAGTATGTATCGGACCCGCAAAATTCCCTGAAAGAGCATATCGACCAGCTGTGGCCGGTg      >  2:2487451/1‑100 (MQ=255)

                                                                                            gttgGAAACCACTTCTGGCTGCCGGAGGTCTACTCCAGCGAGTATGTATCGGACCCGCAAAATTCCCTGAAAGAGCATATCGACCAGCTGTGGCCGGTGc     <  2:2090886/100‑1 (MQ=255)

                                                                                            gttgGAAACCACTTCTGGCTGCCGGAGGTCTACTCCAGCGAGTATGTATCGGACCCGCAAAATTCCCTGAAAGAGCATATCGACCAGCTGTGGCCGGTGc     <  2:809067/100‑1 (MQ=255)

                                                                                            gttgGAAACCACTTCTGGCTGCCGGAGGTCTACTCCAGCGAGTATGTATCGGACCCGCAAAATTCCCTGAAAGAGCATATCGACCAGCTGTGGCCGGTGc     >  1:2888886/1‑100 (MQ=255)

                                                                                            gttgGAAACCACTTCTGGCTGCCGGAGGTCTACTCCAGCGAGTATGTATCGGACCCGCAAAATTCCCTGAAAGAGCATATCGACCAGCTGTGGCCGGTGc     <  1:2866171/100‑1 (MQ=255)

                                                                                             ttgGAAACCACTTCTGGCTGCCGGAGGTCTACTCCAGCGAGTATGTATCGGACCCGCAAAATTCCCTGAAAGAGCATATCGACCAGCTGTGGCCGGTGCt    >  1:1524746/1‑100 (MQ=255)

TTCCCGACTGCGCACCTAAAATGGACCCGCTGGATATCTTAATCCGCTACCGTAAAGTGCGCCGTCATCGTGATTTTGACTTGCGCAAGTTTGTTGAAAACCACTTCTGGCTGCCGGAGGTCTACTCCAGCGAGTATGTATCGGACCCGCAAAATTCCCTGAAAGAGCATATCGACCAGCTGTGGCCGGTGCTAA  >  NC_000913/3669824‑3670018

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`