Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,705,970 G→A 21.8% intergenic (‑180/+128) dppB ← / ← dppA dipeptide/heme ABC transporter permease/dipeptide/heme ABC transporter periplasmic binding protein; dipeptide chemotaxis receptor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,705,9700GA21.8% 243.0 / 62.6 125intergenic (‑180/+128)dppB/dppAdipeptide/heme ABC transporter permease/dipeptide/heme ABC transporter periplasmic binding protein; dipeptide chemotaxis receptor
Reads supporting (aligned to +/- strand):  ref base G (47/50);  new base A (20/7);  total (67/58)
Fisher's exact test for biased strand distribution p-value = 2.79e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.77e-03

Only 100 of 139 total aligned reads displayed.

CAGGGAATGAAGTATTGCTGCCGGATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCATTTTTGCC  >  NC_000913/3705871‑3706069
                                                                                                   |                                                                                                     
  gggAATGAAGTATTGCTGCCGGATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATgcg                                                                                                     >  2:2443298/1‑100 (MQ=255)
   ggAATGAAGTATTGCTGCCGGATGGCGCTGCACGCAGAGGCACAGCGCCACCGTGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATgcgg                                                                                                    <  2:1680287/100‑1 (MQ=255)
    gAATGAAGTATTGCTGCCGGATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATgcggc                                                                                                   >  1:1039805/1‑100 (MQ=255)
    gAATGAAGTATTGCTGCCGGATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATgcggc                                                                                                   <  2:893962/100‑1 (MQ=255)
     aaTGAAGTATTGCTGCCGGATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATgcggcg                                                                                                  <  2:2948598/100‑1 (MQ=255)
        gAAGTATTGCTGCCGGATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTa                                                                                               <  2:639291/100‑1 (MQ=255)
         aaGTATTGCTGCCGGATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTaa                                                                                              >  2:773845/1‑100 (MQ=255)
             aTTGCTGCCGGATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGcc                                                                                          <  2:250664/100‑1 (MQ=255)
              ttGCTGCCGGATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCt                                                                                         <  1:926365/100‑1 (MQ=255)
              ttGCTGCCGGATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCt                                                                                         <  1:1400657/100‑1 (MQ=255)
              ttGCTGCCGGATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCt                                                                                         <  1:2611395/100‑1 (MQ=255)
              ttGCTGCCGGATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCt                                                                                         <  1:1707229/100‑1 (MQ=255)
              ttGCTGCCGGATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCt                                                                                         <  1:2312421/100‑1 (MQ=255)
                gctgcCGGATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTa                                                                                       >  1:2994121/1‑100 (MQ=255)
                gctgcCGGATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTa                                                                                       <  2:697540/100‑1 (MQ=255)
                  tgcCGGATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATc                                                                                     >  2:1832441/1‑100 (MQ=255)
                   gcCGGATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATcc                                                                                    >  1:1381441/1‑100 (MQ=255)
                   gcCGGATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATcc                                                                                    <  2:1827955/100‑1 (MQ=255)
                      ggATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCAGGc                                                                                 >  1:670220/1‑100 (MQ=255)
                      ggATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGACGTTAACGCCTTATcc                                                                                    >  1:3015248/1‑97 (MQ=255)
                      ggATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATAAGGCGTTAACGCCGCATCCGGc                                                                                 <  2:1736085/100‑1 (MQ=37)
                       gATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGcc                                                                                <  2:19662/100‑1 (MQ=255)
                       gATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGCTAACGCCTTATCCGGcc                                                                                >  2:75192/1‑100 (MQ=255)
                        aTGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATAAGGCGTTAACGCCGCATCCGGcat                                                                               <  1:2149237/100‑3 (MQ=37)
                        aTGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATAAGGCGTTAACGCCGCATCCGGcat                                                                               <  1:797027/100‑3 (MQ=37)
                        aTGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATAAGGCGTTAACGCCGCATCCGGcat                                                                               <  1:807423/100‑3 (MQ=37)
                        aTGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATAAGGCGTTAACGCCGCATCCGGcat                                                                               <  1:300444/100‑3 (MQ=37)
                         ttgcgcTGCACGTATAGGCACAGCGTCTTTGGGTATATAGATTGTTGACAAAGTGCGCGTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGc                                                                                 <  1:368827/95‑1 (MQ=17)
                           gcgcTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGAATGCGGCGTTAACGCCTTATCCGGCCTACa                                                                            >  1:1012399/1‑100 (MQ=255)
                            cgcTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACaa                                                                           >  1:1569909/1‑100 (MQ=255)
                            cgcTGCACGCAGAGGCACAGCACCACCGGGTATACAGATTGTTGACAAACTGCGCTTTGTTTATGCCGGATGCCGCGTTAACGCCTTATCCGTCCTACaa                                                                           >  2:2615250/1‑100 (MQ=25)
                                 cACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAAAGCCTTATCCGGCCTACAAAATCg                                                                      >  1:468003/1‑100 (MQ=255)
                                  aCGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGt                                                                     >  1:1186042/1‑100 (MQ=255)
                                  aCGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGt                                                                     <  2:2952231/100‑1 (MQ=255)
                                   cGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGt                                                                     >  1:1415153/1‑99 (MQ=255)
                                   cGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTg                                                                    >  2:1958097/1‑100 (MQ=255)
                                   cGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTg                                                                    <  2:306123/100‑1 (MQ=255)
                                   cGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCAGCCTACAAAATCGt                                                                     >  1:1070066/1‑99 (MQ=255)
                                     cAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCa                                                                  <  1:2709322/100‑1 (MQ=255)
                                        agGCACAGCGCCACCGGGTATATAGTTTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAAt                                                               <  1:2117489/100‑1 (MQ=255)
                                        agGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAAt                                                               <  2:2172688/100‑1 (MQ=255)
                                          gCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTc                                                             >  2:1190205/1‑100 (MQ=255)
                                             caGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAAt                                                          <  1:2767584/100‑1 (MQ=255)
                                             caGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAAt                                                          <  2:1050109/100‑1 (MQ=255)
                                              aGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATa                                                         <  2:1634128/100‑1 (MQ=255)
                                                 gcCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAGAACGTGCAAATTCaacaaat                                                      >  2:1919836/1‑95 (MQ=17)
                                                 gcCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCAGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACAt                                                      >  1:1493294/1‑100 (MQ=255)
                                                     ccGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCa                                                  <  2:1364976/100‑1 (MQ=255)
                                                      cGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAg                                                 <  2:499872/100‑1 (MQ=255)
                                                        ggTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGACCTACAAAATCGTGCAAATTCAATACATTGCAGaa                                               >  1:1141105/1‑100 (MQ=255)
                                                            taCAGATTGTTGACAAAGTGCGCTTTGTTTATGCCTGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTTCAAATTCAATACATTGCAGAAtttt                                           >  2:117613/1‑100 (MQ=255)
                                                            taCAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAAtttt                                           >  1:986737/1‑100 (MQ=255)
                                                              cAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTC‑‑g                                       >  1:1702126/1‑100 (MQ=255)
                                                              cAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTC‑‑g                                       <  2:2293156/100‑1 (MQ=255)
                                                              cAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTC‑‑g                                       >  1:2857584/1‑100 (MQ=255)
                                                               aGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTC‑‑Gt                                      >  1:701618/1‑100 (MQ=255)
                                                                gATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCATATTCAATACATTGCAGAATTTTC‑‑GTa                                     <  1:1843893/100‑1 (MQ=255)
                                                                gATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTC‑‑GTa                                     <  2:371916/100‑1 (MQ=255)
                                                                  ttgttgACAAAGTGCGCTTTGTTTATGCCGGATGCTGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTC‑‑GTAgg                                   <  1:1201207/100‑1 (MQ=255)
                                                                  ttgttgACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTC‑‑GTAgg                                   <  2:546365/100‑1 (MQ=255)
                                                                     ttgACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTC‑‑GTATGCCt                                >  2:1931382/1‑100 (MQ=255)
                                                                      tgACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTC‑‑GTAGGCCTg                               >  2:759821/1‑100 (MQ=255)
                                                                       gACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTC‑‑GTAGGCCTGa                              >  2:1712624/1‑100 (MQ=255)
                                                                        aCAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTC‑‑GTAGGCCTGAt                             <  2:2811126/100‑1 (MQ=255)
                                                                        aCAAAGTGCGCTTTGTTTATGCAGGATGCGGCGTTAAAGCCTTATCAGGCATACAAAATCGTGCAAATTCAATACATTGCAGAATTTTC‑‑GTAGGCCTGAt                             >  2:2140109/1‑100 (MQ=18)
                                                                           aaGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTC‑‑GTAGGCCTGATAAg                          <  1:2655613/100‑1 (MQ=255)
                                                                            aGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTC‑‑GTAGGCCTGATAAGc                         >  1:2827266/1‑100 (MQ=255)
                                                                              tGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTC‑‑GTAGGCCTGATAAGCgg                       <  1:330353/100‑1 (MQ=255)
                                                                               gcgcTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTC‑‑GTAGGCCTGATCAGCGGa                      >  2:1217667/1‑100 (MQ=255)
                                                                                cgcTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTC‑‑GTAGGCCTGATAAGCGGAg                     >  1:587145/1‑100 (MQ=255)
                                                                                cgcTTTGTTGATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTC‑‑GTAGGCCTGATAAGCGGAg                     <  2:2570162/100‑1 (MQ=255)
                                                                                 gcTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTC‑‑GTAGGCCTGATAAGCGGAgc                    <  2:459978/100‑1 (MQ=255)
                                                                                 gcTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTC‑‑GTAGGCCTGATAAGCGGAgc                    >  2:373331/1‑100 (MQ=255)
                                                                                  cTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTC‑‑GTAGGCCTGATAAGCGGAgc                    <  1:405163/99‑1 (MQ=255)
                                                                                     tttgtaggCCGGATAAGTCGTTCACTCCGCATCCGGCCTACAAAAGCACGCAAATTCAATATATTACAGAGATTGC‑‑GTAGGCCTGATAAGCGTAg                     >  2:1462092/8‑95 (MQ=11)
                                                                                     tGTTTCTGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAAATTTC‑‑GTACGCCTGATAAGCGGAgcgc                  >  2:1938451/1‑98 (MQ=255)
                                                                                      gTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCTTGCAAATTCAATACATTGCAGAAATTTC‑‑GTAGGCCTGATAAGCGGAGCGCATc               >  1:1652348/1‑100 (MQ=255)
                                                                                      gTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTC‑‑GTAGGCCTGATAAGCGGAGCGCATc               <  2:2326732/100‑1 (MQ=255)
                                                                                      gTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTC‑‑GTAGGCCTGATAAGCGGAGCGCATc               >  1:284379/1‑100 (MQ=255)
                                                                                       tgtaggCCGGATAAGGCGTTTACGCCGCATCCGGCCTACAAAATCGTGCAAATTCAACATATTGCAATTCTCTT‑‑GTAGGCCTGATAAGCGTAGCGCATCa              >  1:1798739/6‑100 (MQ=18)
                                                                                        gtaggCCGGATAAGGCGTTCACGCCGCATCCGGCCTACAAAAGCATGAAAATTCAATATATTGCAGGAGCTGC‑‑GTAGGCCTGATAAGCGTAGCGCATCAg             >  2:3020255/5‑100 (MQ=18)
                                                                                        gtaggCCGGATAAGGCGTTCACGCCGCATCCGGCCTACAAAACTTTGCAAATTCAATATATTGC‑‑AATCTCCGTGTAGGCCTGATAAGCGTAGCGCATCAg             <  1:2399970/96‑1 (MQ=18)
                                                                                         taggCCGGATAAGGCGTTCACTCCGCATCCGGCCTACAAAAGCACGCAAATTCAATATATTGCAGAGATTGC‑‑GTAGGCCTGATAAGCGTAGCGCATCAg             >  2:2521256/4‑99 (MQ=14)
                                                                                         taggCCGGATAAGGCGTTCACGCCGCATCCGGCCTACAAAATTGTGTAAATTCAATAAATTGATCGATCCAC‑‑GTAGGCCTGATAAGCGTAGCGCATCAgg            >  2:3026071/4‑100 (MQ=21)
                                                                                         tATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATCCAATACATTGCAGAATTTTC‑‑GTAGGCCTGATAAGCGGAGCGCATAAgg            >  2:1289242/1‑100 (MQ=255)
                                                                                          aggCCGGATAAGGCGTTTACGCCGCATCCGGCCTACAAAAGCGTGCAAATTCAATACATTGCATGGGCCAT‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGc           >  1:412280/3‑100 (MQ=17)
                                                                                          aggCCGGATAAGGCGTTCACGCCGCATCCGGCCTACAAAATCATGAAAATTCAATACATTGCAAGATTTTC‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGc           >  1:396157/3‑100 (MQ=17)
                                                                                           ggCCGGATAAGGCGTTTACGCCGCATCCGGCCTACAAAATCGTGCTAATTCAATATATTGCAGAAACCCT‑‑GTAGGCCGGATAAGCGTAGCGCATCAGGCa          >  2:2050239/2‑100 (MQ=17)
                                                                                           ggCCGGATAAGGCGTTTACGCCGCATCCGGCCTACAAAAGCATGCAAATTCAATATATTGCAGAGTTCAC‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGCa          >  1:85464/2‑100 (MQ=14)
                                                                                           ggCCGGATAAGGCGTTCACGCCGCATCCGGCCTACAAAGTCGTATAAATTCAATGTATTGCAGTTTATTG‑‑GTAGGCCTGATAAGCGCAGCGCATCAGGCa          >  1:509184/2‑100 (MQ=21)
                                                                                           ggCCGGATAAGGCGTTCACGCCGCATCCGGCCTACAAAAACCTGCAAATTCAATATATTGCAGGATACGT‑‑ATAGGCCTGATAAGCGTAGCGCATCAGGCa          >  1:321438/2‑100 (MQ=17)
                                                                                            gCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCAt         >  1:370551/1‑100 (MQ=255)
                                                                                            gCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCAt         >  1:1233024/1‑100 (MQ=255)
                                                                                            gCCGGATAAGGCGTTCACGCCGCATCCGGCCTACACAAACATGCAAATTCAATAGATTGCAGAGATTAT‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGCaa         >  1:25540/1‑99 (MQ=17)
                                                                                            gCCGGATAAGGCGTTCACGCCGCATCCGGCCTACAAGTTCGTGCAAATTCAATAAATTGCAATATGAC‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGcgat        >  1:494082/1‑97 (MQ=21)
                                                                                            gCCGGATAAGGCGTTCACGCCGCATCCGGCCTACAAAACTTTGCAAATTCAATATATTGC‑‑AATCTCCGTGTAGGCCTGATAAGCGTAGCGCATCAGGCaa         >  1:2262768/1‑99 (MQ=18)
                                                                                             ccGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCAtt        >  1:2028258/1‑100 (MQ=255)
                                                                                             ccGGATCCGGCGTTAACGCCTTATCCAGCCTACAAAATTGTGCAAAATCAATTGATTGCACAGCTAGC‑‑GTAGGCCTGATGCGCTACGCTCATCAGGc           <  2:1483766/97‑1 (MQ=18)
                                                                                                 aTGCGGCGTTAACGCCTTATCCGGCCTACAAATTCGTGCAAATTCAATACATTGCAGAATTTTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCATTtttg    <  2:89473/100‑1 (MQ=255)
                                                                                                   gcggcgTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCATTtttgcc  >  1:895262/1‑100 (MQ=255)
                                                                                                   |                                                                                                     
CAGGGAATGAAGTATTGCTGCCGGATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCATTTTTGCC  >  NC_000913/3705871‑3706069

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: