Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,705,971 C→A 22.0% intergenic (‑181/+127) dppB ← / ← dppA dipeptide/heme ABC transporter permease/dipeptide/heme ABC transporter periplasmic binding protein; dipeptide chemotaxis receptor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,705,9710CA22.0% 261.8 / 74.8 125intergenic (‑181/+127)dppB/dppAdipeptide/heme ABC transporter permease/dipeptide/heme ABC transporter periplasmic binding protein; dipeptide chemotaxis receptor
Reads supporting (aligned to +/- strand):  ref base C (47/50);  new base A (20/7);  total (67/58)
Fisher's exact test for biased strand distribution p-value = 2.79e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.48e-04

Only 100 of 140 total aligned reads displayed.

AGGGAATGAAGTATTGCTGCCGGATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCATTTTTGCCT  >  NC_000913/3705872‑3706070
                                                                                                   |                                                                                                     
 gggAATGAAGTATTGCTGCCGGATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATgcg                                                                                                      >  1:332633/1‑100 (MQ=255)
   gAATGAAGTATTGCTGCCGGATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATgcggc                                                                                                    >  1:1039805/1‑100 (MQ=255)
   gAATGAAGTATTGCTGCCGGATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATgcggc                                                                                                    <  2:893962/100‑1 (MQ=255)
    aaTGAAGTATTGCTGCCGGATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATgcggcg                                                                                                   <  2:2948598/100‑1 (MQ=255)
    aaTGAAGTATTGCTGCCGGATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATgcggcg                                                                                                   <  1:813899/100‑1 (MQ=255)
       gAAGTATTGCTGCCGGATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTa                                                                                                <  2:639291/100‑1 (MQ=255)
        aaGTATTGCTGCCGGATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTaa                                                                                               >  2:773845/1‑100 (MQ=255)
            aTTGCTGCCGGATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGcc                                                                                           <  2:250664/100‑1 (MQ=255)
             ttGCTGCCGGATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCt                                                                                          <  1:2312421/100‑1 (MQ=255)
             ttGCTGCCGGATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCt                                                                                          <  1:1400657/100‑1 (MQ=255)
             ttGCTGCCGGATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCt                                                                                          <  1:2611395/100‑1 (MQ=255)
             ttGCTGCCGGATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCt                                                                                          <  1:1533050/100‑1 (MQ=255)
             ttGCTGCCGGATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCt                                                                                          <  1:1707229/100‑1 (MQ=255)
             ttGCTGCCGGATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCt                                                                                          <  1:926365/100‑1 (MQ=255)
               gctgcCGGATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTa                                                                                        >  1:2994121/1‑100 (MQ=255)
               gctgcCGGATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTa                                                                                        <  2:697540/100‑1 (MQ=255)
                 tgcCGGATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATc                                                                                      >  2:1832441/1‑100 (MQ=255)
                  gcCGGATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATcc                                                                                     <  2:1827955/100‑1 (MQ=255)
                  gcCGGATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATcc                                                                                     >  1:1381441/1‑100 (MQ=255)
                     ggATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCAGGc                                                                                  >  1:670220/1‑100 (MQ=255)
                     ggATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGACGTTAACGCCTTATcc                                                                                     >  1:3015248/1‑97 (MQ=255)
                     ggATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATAAGGCGTTAACGCCGCATCCGGc                                                                                  <  2:1736085/100‑1 (MQ=37)
                      gATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGcc                                                                                 <  2:19662/100‑1 (MQ=255)
                      gATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGCTAACGCCTTATCCGGcc                                                                                 >  2:75192/1‑100 (MQ=255)
                       aTGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATAAGGCGTTAACGCCGCATCCGGcat                                                                                <  1:807423/100‑3 (MQ=37)
                       aTGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATAAGGCGTTAACGCCGCATCCGGcat                                                                                <  1:2149237/100‑3 (MQ=37)
                        ttgcgcTGCACGTATAGGCACAGCGTCTTTGGGTATATAGATTGTTGACAAAGTGCGCGTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGc                                                                                  <  1:368827/95‑1 (MQ=17)
                        tGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTa                                                                               <  2:139529/100‑1 (MQ=255)
                          gcgcTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGAATGCGGCGTTAACGCCTTATCCGGCCTACa                                                                             >  1:1012399/1‑100 (MQ=255)
                           cgcTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACaa                                                                            >  1:1569909/1‑100 (MQ=255)
                           cgcTGCACGCAGAGGCACAGCACCACCGGGTATACAGATTGTTGACAAACTGCGCTTTGTTTATGCCGGATGCCGCGTTAACGCCTTATCCGTCCTACaa                                                                            >  2:2615250/1‑100 (MQ=25)
                                cACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAAAGCCTTATCCGGCCTACAAAATCg                                                                       >  1:468003/1‑100 (MQ=255)
                                 aCGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGt                                                                      >  1:1186042/1‑100 (MQ=255)
                                 aCGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGt                                                                      <  2:2952231/100‑1 (MQ=255)
                                  cGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGt                                                                      >  1:1415153/1‑99 (MQ=255)
                                  cGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTg                                                                     <  2:306123/100‑1 (MQ=255)
                                  cGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTg                                                                     >  2:1958097/1‑100 (MQ=255)
                                  cGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCAGCCTACAAAATCGt                                                                      >  1:1070066/1‑99 (MQ=255)
                                    cAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCa                                                                   <  1:2709322/100‑1 (MQ=255)
                                       agGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAAt                                                                <  2:2172688/100‑1 (MQ=255)
                                         gCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTc                                                              >  2:1190205/1‑100 (MQ=255)
                                            caGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAAt                                                           <  1:2767584/100‑1 (MQ=255)
                                            caGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAAt                                                           <  2:1050109/100‑1 (MQ=255)
                                             aGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATa                                                          <  2:1634128/100‑1 (MQ=255)
                                              gcgcCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATAc                                                         <  1:46985/100‑1 (MQ=255)
                                                    ccGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCa                                                   <  2:1364976/100‑1 (MQ=255)
                                                     cGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAg                                                  <  2:499872/100‑1 (MQ=255)
                                                       ggTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGACCTACAAAATCGTGCAAATTCAATACATTGCAGaa                                                >  1:1141105/1‑100 (MQ=255)
                                                           taCAGATTGTTGAAAAAGTGCGCTTTGTTTATGCCGGACGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAAtttt                                            >  2:1871446/1‑100 (MQ=255)
                                                             cAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTC‑‑g                                        <  2:2293156/100‑1 (MQ=255)
                                                              aGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTC‑‑Gt                                       >  1:701618/1‑100 (MQ=255)
                                                               gATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCATATTCAATACATTGCAGAATTTTC‑‑GTa                                      <  1:1843893/100‑1 (MQ=255)
                                                               gATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTC‑‑GTa                                      <  2:371916/100‑1 (MQ=255)
                                                                 ttgttgACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTC‑‑GTAgg                                    <  2:546365/100‑1 (MQ=255)
                                                                   gttgACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGTTTTATCCTGTTTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTC‑‑GTAGGcc                                  <  1:983872/100‑1 (MQ=18)
                                                                    ttgACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTC‑‑GTATGCCt                                 >  2:1931382/1‑100 (MQ=255)
                                                                     tgACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTC‑‑GTAGGCCTg                                >  2:759821/1‑100 (MQ=255)
                                                                      gACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTC‑‑GTAGGCCTGa                               >  2:1712624/1‑100 (MQ=255)
                                                                       aCAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTC‑‑GTAGGCCTGAt                              <  2:2811126/100‑1 (MQ=255)
                                                                       aCAAAGTGCGCTTTGTTTATGCAGGATGCGGCGTTAAAGCCTTATCAGGCATACAAAATCGTGCAAATTCAATACATTGCAGAATTTTC‑‑GTAGGCCTGAt                              >  2:2140109/1‑100 (MQ=18)
                                                                          aaGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTC‑‑GTAGGCCTGATAAg                           <  1:2655613/100‑1 (MQ=255)
                                                                             tGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTC‑‑GTAGGCCTGATAAGCgg                        <  1:330353/100‑1 (MQ=255)
                                                                              gcgcTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTC‑‑GTAGGCCTGATCAGCGGa                       >  2:1217667/1‑100 (MQ=255)
                                                                               cgcTTTGTTGATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTC‑‑GTAGGCCTGATAAGCGGAg                      <  2:2570162/100‑1 (MQ=255)
                                                                                gcTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTC‑‑GTAGGCCTGATAAGCGGAgc                     >  2:373331/1‑100 (MQ=255)
                                                                                gcTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTC‑‑GTAGGCCTGATAAGCGGAgc                     <  2:459978/100‑1 (MQ=255)
                                                                                 cTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTC‑‑GTAGGCCTGATAAGCGGAgcg                    <  1:3040692/100‑1 (MQ=255)
                                                                                  tttGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTC‑‑GTAGGCCTGATAAGCGGAgcgc                   >  1:746255/1‑100 (MQ=255)
                                                                                    tttgtaggCCGGATAAGGCGTTTACGCCGCATCCGGCCTACAAAATCGTGCAAATTCAACATATTGCAATTCTCTT‑‑GTAGGCCTGATAAGCGTAGCGCAt                 >  1:2836239/8‑100 (MQ=18)
                                                                                    tGTTTCTGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAAATTTC‑‑GTACGCCTGATAAGCGGAgcgc                   >  2:1938451/1‑98 (MQ=255)
                                                                                    tGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACAATGCAGAATTTTC‑‑GTAGGCCTGATAAGCGGAGCGCAt                 >  2:1671444/1‑100 (MQ=255)
                                                                                     ttgtaggCCGGATAAGGCGTTCACGCCGCATCCGGCCTACAAAACTTTGCAAATTCAATATATTGC‑‑AATCTCCGTGTAGGCCTGATAAGCGTAGCGCATc                >  2:2399942/7‑100 (MQ=18)
                                                                                     gTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCTTGCAAATTCAATACATTGCAGAAATTTC‑‑GTAGGCCTGATAAGCGGAGCGCATc                >  1:1652348/1‑100 (MQ=255)
                                                                                     gTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGGATTTTC‑‑GTAGGCCTGATCAGCGGAGCGCATc                <  1:2044564/100‑1 (MQ=255)
                                                                                     gTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTC‑‑GTAGGCCTGATAAGCGGAGCGCATc                >  1:284379/1‑100 (MQ=255)
                                                                                     gTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTC‑‑GTAGGCCTGATAAGCGGAGCGCATc                <  2:2326732/100‑1 (MQ=255)
                                                                                      tttATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTC‑‑GTAGGCCTGATAAGCGGAGCGAATCa               >  1:577411/1‑100 (MQ=255)
                                                                                      tgtaggCCGGATAAGGCGTTTACGCCGCATCCGGCCTACAAAATCGTGCAAATTCAACATATTGCAATTCTCTT‑‑GTAGGCCTGATAAGCGTAGCGCATCa               >  1:1798739/6‑100 (MQ=18)
                                                                                       gtaggCCGGATAAGGCGTTCACGCCGCATCCGGCCTACAAAAGCATGAAAATTCAATATATTGCAGGAGCTGC‑‑GTAGGCCTGATAAGCGTAGCGCATCAg              >  2:3020255/5‑100 (MQ=18)
                                                                                       gtaggCCGGATAAGGCGTTCACGCCGCATCCGGCCTACAAAACTTTGCAAATTCAATATATTGC‑‑AATCTCCGTGTAGGCCTGATAAGCGTAGCGCATCAg              <  1:2399970/96‑1 (MQ=18)
                                                                                        taggCCGGATAAGGCGTTCACTCCGCATCCGGCCTACAAAAGCACGCAAATTCAATATATTGCAGAGATTGC‑‑GTAGGCCTGATAAGCGTAGCGCATCAg              >  2:2521256/4‑99 (MQ=14)
                                                                                        taggCCGGATAAGGCGTTCACGCCGCATCCGGCCTACAAAATTGTGTAAATTCAATAAATTGATCGATCCAC‑‑GTAGGCCTGATAAGCGTAGCGCATCAgg             >  2:3026071/4‑100 (MQ=21)
                                                                                         aggCCGGATAAGGCGTTTACGCCGCATCCGGCCTACAAAAGCGTGCAAATTCAATACATTGCATGGGCCAT‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGc            >  1:412280/3‑100 (MQ=17)
                                                                                         aggCCGGATAAGGCGTTCACGCCGCATCCGGCCTACAAAATCATGAAAATTCAATACATTGCAAGATTTTC‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGc            >  1:396157/3‑100 (MQ=17)
                                                                                          ggCCGGATAAGGCGTTCACGCCGCATCCGGCCTACAAAGTCGTATAAATTCAATGTATTGCAGTTTATTG‑‑GTAGGCCTGATAAGCGCAGCGCATCAGGCa           >  1:509184/2‑100 (MQ=21)
                                                                                          ggCCGGATAAGGCGTTCACGCCGCATCCGGCCTACAAAATTGTGTAAATTCAATAAATTGATCGATCCAC‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGCa           >  2:1106044/2‑100 (MQ=21)
                                                                                          ggCCGGATAAGGCGTTCACGCCGCATCCGGCCTACAAAACCTTGCTAATTCAATATATTGCAGGGACTAT‑‑GTAGACCTGATAAGCATAGCGCATCAGGCa           >  1:1212855/2‑100 (MQ=18)
                                                                                          ggCCGGATAAGGCGTTCACGCCGCATCCGGCCTACAAAACCTTGCTAATTCAATATATTGCAGAGACTAT‑‑GTAGGCCTGATAAGCATAGCGCATCAGGCa           >  1:401355/2‑100 (MQ=17)
                                                                                           gCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCAt          >  1:1233024/1‑100 (MQ=255)
                                                                                           gCCGGATAAGGCGTTCACGCCGCATCCGGCCTACACAAACATGCAAATTCAATAGATTGCAGAGATTAT‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGCaa          >  1:25540/1‑99 (MQ=17)
                                                                                           gCCGGATAAGGCGTTCACGCCGCATCCGGCCTACAAAACTTTGCAAATTCAATATATTGC‑‑AATCTCCGTGTAGGCCTGATAAGCGTAGCGCATCAGGCaa          >  1:2262768/1‑99 (MQ=18)
                                                                                            ccGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCAtt         >  1:2028258/1‑100 (MQ=255)
                                                                                            ccGGATCCGGCGTTAACGCCTTATCCAGCCTACAAAATTGTGCAAAATCAATTGATTGCACAGCTAGC‑‑GTAGGCCTGATGCGCTACGCTCATCAGGc            <  2:1483766/97‑1 (MQ=18)
                                                                                              ggATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCAtttt       <  1:2969638/100‑1 (MQ=255)
                                                                                                aTGGGGCGTTATCGCTTTATAGGGCGAAAAAAATCGTGGAAATTCACTACATTGCAGAATTTTC‑‑GGAGGGCTAATAAGTGGAGCGCATCAGGCATTtttg     <  2:1974961/100‑1 (MQ=16)
                                                                                                aTGCGGGGTTAACTCCTTATCCGGCATACAAAATGGTGCAAATTCAATACATTGCAGAATTTTC‑‑GTAGGCCTTATAAGCGGAGTGCATCAGGGATTtttg     <  2:1270139/100‑1 (MQ=18)
                                                                                                aTGCGGCGTTAACGCCTTATCCGGCCTACAAATTCGTGCAAATTCAATACATTGCAGAATTTTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCATTtttg     <  2:89473/100‑1 (MQ=255)
                                                                                                  gcggcgTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCATTtttgcc   >  1:895262/1‑100 (MQ=255)
                                                                                                   cggcgTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCATTtttgcct  <  1:2913962/100‑1 (MQ=255)
                                                                                                   cggcgTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCATTtttgcct  >  1:2748791/1‑100 (MQ=255)
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AGGGAATGAAGTATTGCTGCCGGATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCATTTTTGCCT  >  NC_000913/3705872‑3706070

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: