Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,705,984 T→G 31.4% intergenic (‑194/+114) dppB ← / ← dppA dipeptide/heme ABC transporter permease/dipeptide/heme ABC transporter periplasmic binding protein; dipeptide chemotaxis receptor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,705,9840TG31.4% 238.0 / 156.0 153intergenic (‑194/+114)dppB/dppAdipeptide/heme ABC transporter permease/dipeptide/heme ABC transporter periplasmic binding protein; dipeptide chemotaxis receptor
Reads supporting (aligned to +/- strand):  ref base T (55/50);  new base G (40/8);  total (95/58)
Fisher's exact test for biased strand distribution p-value = 2.78e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.34e-04

Only 100 of 160 total aligned reads displayed.

TTGCTGCCGGATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCATTTTTGCCTTTGCCATCAGT  >  NC_000913/3705885‑3706081
                                                                                                   |                                                                                                     
ttGCTGCCGGATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCt                                                                                                       <  1:1400657/100‑1 (MQ=255)
ttGCTGCCGGATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCt                                                                                                       <  1:2611395/100‑1 (MQ=255)
ttGCTGCCGGATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCt                                                                                                       <  1:1533050/100‑1 (MQ=255)
ttGCTGCCGGATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCt                                                                                                       <  1:2312421/100‑1 (MQ=255)
    tgcCGGATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATc                                                                                                   >  2:1832441/1‑100 (MQ=255)
        ggATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATAAGGCGTTAACGCCGCATCCGGc                                                                                               <  2:1736085/100‑1 (MQ=37)
         gATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGCTAACGCCTTATCCGGcc                                                                                              >  2:75192/1‑100 (MQ=255)
          aTGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATAAGGCGTTAACGCCGCATCCGGcat                                                                                             <  1:797027/100‑3 (MQ=37)
          aTGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATAAGGCGTTAACGCCGCATCCGGcat                                                                                             <  1:300444/100‑3 (MQ=37)
          aTGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATAAGGCGTTAACGCCGCATCCGGcat                                                                                             <  1:2149237/100‑3 (MQ=37)
           ttgcgcTGCACGTATAGGCACAGCGTCTTTGGGTATATAGATTGTTGACAAAGTGCGCGTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGc                                                                                               <  1:368827/95‑1 (MQ=17)
           tGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTa                                                                                            <  2:139529/100‑1 (MQ=255)
             gcgcTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGAATGCGGCGTTAACGCCTTATCCGGCCTACa                                                                                          >  1:1012399/1‑100 (MQ=255)
              cgcTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACaa                                                                                         >  1:1569909/1‑100 (MQ=255)
              cgcTGCACGCAGAGGCACAGCACCACCGGGTATACAGATTGTTGACAAACTGCGCTTTGTTTATGCCGGATGCCGCGTTAACGCCTTATCCGTCCTACaa                                                                                         >  2:2615250/1‑100 (MQ=25)
                  gCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATAAGGCGTTAACGCCGCATCCGGcataaacaaag                                                                                     <  2:2842761/100‑11 (MQ=21)
                   cACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAAAGCCTTATCCGGCCTACAAAATCg                                                                                    >  1:468003/1‑100 (MQ=255)
                    aCGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGt                                                                                   <  2:2952231/100‑1 (MQ=255)
                     cGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGt                                                                                   >  1:1415153/1‑99 (MQ=255)
                     cGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTg                                                                                  >  2:1958097/1‑100 (MQ=255)
                     cGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTg                                                                                  <  2:306123/100‑1 (MQ=255)
                       cAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCa                                                                                <  1:2709322/100‑1 (MQ=255)
                          agGCACAGCGCCACCGGGTATATAGTTTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAAt                                                                             <  1:2117489/100‑1 (MQ=255)
                          agGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAAt                                                                             <  2:2172688/100‑1 (MQ=255)
                            gCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTc                                                                           >  2:1190205/1‑100 (MQ=255)
                               caGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAAt                                                                        <  1:2767584/100‑1 (MQ=255)
                               caGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAAt                                                                        <  2:1050109/100‑1 (MQ=255)
                                aGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATa                                                                       <  2:1634128/100‑1 (MQ=255)
                                 gcgcCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATAc                                                                      <  1:46985/100‑1 (MQ=255)
                                 gcgcCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATAc                                                                      <  2:45238/100‑1 (MQ=255)
                                   gcCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAGAACGTGCAAATTCaacaaat                                                                    >  2:1919836/1‑95 (MQ=17)
                                        cGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAg                                                              <  2:499872/100‑1 (MQ=255)
                                          ggTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGACCTACAAAATCGTGCAAATTCAATACATTGCAGaa                                                            >  1:1141105/1‑100 (MQ=255)
                                              taCAGATTGTTGACAAAGTGCGCTTTGTTTATGCCTGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTTCAAATTCAATACATTGCAGAAtttt                                                       >  2:117613/1‑100 (MQ=255)
                                              taCAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAAtttt                                                       >  1:986737/1‑100 (MQ=255)
                                              taCAGATTGTTGACAAAGTGCGCTTTGTTTATCACGGATGCAGCGTTAACTCCTTATCCGGCCTACAAAATCGTGCAAATTCATTACATTGCAGAAtttt                                                       <  2:85273/100‑1 (MQ=17)
                                                cAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTC‑‑g                                                   >  1:1702126/1‑100 (MQ=255)
                                                 aGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTC‑‑Gt                                                  >  1:701618/1‑100 (MQ=255)
                                                  gATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCATATTCAATACATTGCAGAATTTTC‑‑GTa                                                 <  1:1843893/100‑1 (MQ=255)
                                                  gATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTC‑‑GTa                                                 <  2:371916/100‑1 (MQ=255)
                                                    ttgttgACAAAGTGCGCTTTGTTTATGCCGGATGCTGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTC‑‑GTAgg                                               <  1:1201207/100‑1 (MQ=255)
                                                    ttgttgACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTC‑‑GTAgg                                               <  2:546365/100‑1 (MQ=255)
                                                      gttgACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGTTTTATCCTGTTTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTC‑‑GTAGGcc                                             <  1:983872/100‑1 (MQ=18)
                                                       ttgACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTC‑‑GTATGCCt                                            >  2:1931382/1‑100 (MQ=255)
                                                        tgACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTC‑‑GTAGGCCTg                                           >  2:759821/1‑100 (MQ=255)
                                                          aCAAAGTGCGCTTTGTTTATGCAGGATGCGGCGTTAAAGCCTTATCAGGCATACAAAATCGTGCAAATTCAATACATTGCAGAATTTTC‑‑GTAGGCCTGAt                                         >  2:2140109/1‑100 (MQ=18)
                                                             aaGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTC‑‑GTAGGCCTGATAAg                                      <  1:2655613/100‑1 (MQ=255)
                                                                tGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTC‑‑GTAGGCCTGATAAGCgg                                   <  1:330353/100‑1 (MQ=255)
                                                                  cgcTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTC‑‑GTAGGCCTGATAAGCGGAg                                 >  1:587145/1‑100 (MQ=255)
                                                                   gcTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTC‑‑GTAGGCCTGATAAGCGGAgc                                <  2:459978/100‑1 (MQ=255)
                                                                    cTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTAGAAAATCGTGCAAATTCAATACATTGCAGAATTTTC‑‑GTAGGCCTGATAAGCGGAgcg                               <  2:1944875/100‑1 (MQ=255)
                                                                    cTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTC‑‑GTAGGCCTGATAAGCGGAgcg                               <  1:3040692/100‑1 (MQ=255)
                                                                    cTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTC‑‑GTAGGCCTGATAAGCGGAgc                                <  1:405163/99‑1 (MQ=255)
                                                                     tttGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTC‑‑GTAGGCCTGATAAGCGGAgcgc                              >  1:746255/1‑100 (MQ=255)
                                                                       tttgtaggCCGGATAAGGCGTTTACGCCGCATCCGGCCTACAAAATCGTGCAAATTCAACATATTGCAATTCTCTT‑‑GTAGGCCTGATAAGCGTAGCGCAt                            >  1:2836239/8‑100 (MQ=18)
                                                                        gTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCTTGCAAATTCAATACATTGCAGAAATTTC‑‑GTAGGCCTGATAAGCGGAGCGCATc                           >  1:1652348/1‑100 (MQ=255)
                                                                        gTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTC‑‑GTAGGCCTGATAAGCGGAGCGCATc                           >  1:284379/1‑100 (MQ=255)
                                                                        gTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTC‑‑GTAGGCCTGATAAGCGGAGCGCATc                           <  2:2326732/100‑1 (MQ=255)
                                                                         tttATGCCGGATGCGGCGTTAACGACTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTATC‑‑GTAGGCCTGATAAACGGAGCGCATCa                          >  2:2268461/1‑100 (MQ=255)
                                                                         tgtaggCCGGATAAGGCGTTTACGCCGCATCCGGCCTACAAAATCGTGCAAATTCAACATATTGCAATTCTCTT‑‑GTAGGCCTGATAAGCGTAGCGCATCa                          >  1:1798739/6‑100 (MQ=18)
                                                                          gtaggCCGGATAAGGCGTTCACGCCGCATCCGGCCTACAAAAGCATGAAAATTCAATATATTGCAGGAGCTGC‑‑GTAGGCCTGATAAGCGTAGCGCATCAg                         >  2:3020255/5‑100 (MQ=18)
                                                                           taggCCGGATAAGGCGTTCACTCCGCATCCGGCCTACAAAAGCACGCAAATTCAATATATTGCAGAGATTGC‑‑GTAGGCCTGATAAGCGTAGCGCATCAg                         >  2:2521256/4‑99 (MQ=14)
                                                                            aggCCGGATAAGGCGTTTACGCCGCATCCGGCCTACAAAAGCGTGCAAATTCAATACATTGCATGGGCCAT‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGc                       >  1:412280/3‑100 (MQ=17)
                                                                            aggCCGGATAAGGCGTTCACGCCGCATCCGGCCTACAAAATCATGAAAATTCAATACATTGCAAGATTTTC‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGc                       >  1:396157/3‑100 (MQ=17)
                                                                             ggCCGGATAAGGCGTTTACGCCGCATCCGGCCTACAAAAGCATGCAAATTCAATATATTGCAGAGTTCAC‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGCa                      >  1:85464/2‑100 (MQ=14)
                                                                             ggCCGGATAAGGCGTTCACGCCGCATCCGGCCTACAAAGTCGTATAAATTCAATGTATTGCAGTTTATTG‑‑GTAGGCCTGATAAGCGCAGCGCATCAGGCa                      >  1:509184/2‑100 (MQ=21)
                                                                              gCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCAt                     >  1:1233024/1‑100 (MQ=255)
                                                                              gCCGGATAAGGCGTTCACGCCGCATCCGGCCTACACAAACATGCAAATTCAATAGATTGCAGAGATTAT‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGCaa                     >  1:25540/1‑99 (MQ=17)
                                                                              gCCGGATAAGGCGTTCACGCCGCATCCGGCCTACAAAACTTTGCAAATTCAATATATTGCAATCTCCGTGTAGGCCTGATAAGCGTAGCGCATCAGGCaa                     >  1:2262768/1‑99 (MQ=18)
                                                                               ccggataaggcgTTCACGCCGCATCCGGCCTACGAAATCGTGCTAATTCAATATATTGTGGGCTCCAC‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGCagt                    >  2:945169/9‑98 (MQ=21)
                                                                               ccggataaggcgTTCACGCCGCATCCGGCCTACAAGATCGTGCAAATTCAATATATTGCATGAGCCAC‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGCagt                    >  1:146806/9‑98 (MQ=17)
                                                                               ccggataaggcgTTCACGCCGCATCCGGCCTACAAAACTTTGCAAATTCAATATATTGCAATCTCCGTGTAGGCCTGATAAGCGTAGCGCATCAGGCaat                    >  2:257069/9‑98 (MQ=18)
                                                                               ccGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCAtt                    >  1:2028258/1‑100 (MQ=255)
                                                                               ccGGATCCGGCGTTAACGCCTTATCCAGCCTACAAAATTGTGCAAAATCAATTGATTGCACAGCTAGC‑‑GTAGGCCTGATGCGCTACGCTCATCAGGc                       <  2:1483766/97‑1 (MQ=18)
                                                                                cggataaggcgTTCACGCCGCATCCGGCCTACAAAATCGCTTAAATTCAATATATTGCACCATCCTC‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGCaat                    >  2:1357700/8‑97 (MQ=18)
                                                                                 ggataaggcgTTTACGCCGCATCCGGCCTACAAATTCTGCAAATTCAACAAATTGCGAATCCCTT‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGCAtttgc                 >  1:2576818/7‑98 (MQ=18)
                                                                                 ggataaggcgTTCACGCCGCATCCGGCCTACAAGATCGTGCAAATTCAATATATTGCATGAGCCAC‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGCAgttt                  >  1:131475/7‑100 (MQ=17)
                                                                                 ggataaggcgTTCACGCCGCATCCGGCCTACAAAGTCGTATAAATTCAATGTATTGCAGTTTATTG‑‑GTAGGCCTGATAAGCGCAGCGCATCAGGCaatct                  >  2:1708710/7‑96 (MQ=25)
                                                                                 ggataaggcgTTCACGCCGCATCCGGCCTACAAAAGCATGAAAATTCAATATATTGCAGGAGCTGC‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGCAgtt                   >  1:3034549/7‑99 (MQ=17)
                                                                                 ggataaggcgTTCACGCCGCATCCGGCCTACAAAACCTTGCTAATTCAATATATTGCAGGGACTAT‑‑GTAGGCCTGATAAGCATAGCGCATCAGGCagctt                  >  2:1239319/7‑96 (MQ=21)
                                                                                 ggataaggcgTTCACGCCGCATCCGGCCTACAAATCATGCAAATCCAAAATATTGCAGGAGTTAT‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGCAtttat                 >  1:1066030/7‑98 (MQ=21)
                                                                                 ggataaggcgTTCACGCCGCATCCGGCCCACAAAGTCTTGCAAAATCAATAAATTGCAGGAACGAT‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGCaacta                  >  2:2269240/7‑96 (MQ=21)
                                                                                 ggATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCAtttt                  <  1:2969638/100‑1 (MQ=255)
                                                                                   ataaggcgTTCACGCCGCATCCGGCCTACAAGATCGTGCAAATTCAATATATTGCATGAGCCAC‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGCAGTtttg                <  2:146804/96‑1 (MQ=17)
                                                                                   aTGGGGCGTTATCGCTTTATAGGGCGAAAAAAATCGTGGAAATTCACTACATTGCAGAATTTTC‑‑GGAGGGCTAATAAGTGGAGCGCATCAGGCATTtttg                <  2:1974961/100‑1 (MQ=16)
                                                                                   aTGCGGGGTTAACTCCTTATCCGGCATACAAAATGGTGCAAATTCAATACATTGCAGAATTTTC‑‑GTAGGCCTTATAAGCGGAGTGCATCAGGGATTtttg                <  2:1270139/100‑1 (MQ=18)
                                                                                     gcggcgTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCATTtttgcc              >  1:895262/1‑100 (MQ=255)
                                                                                     gcggcgTTAACGCCTTATCCAGCCTACAAAATAGTACAAAATCAATACATTGCAGAATTTTC‑‑GTAGGCCTGATAAGCGGAGAGCATCAGGCATTtttgcc              >  2:704377/1‑100 (MQ=18)
                                                                                      cggcgTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCATTtttgcct             <  1:2913962/100‑1 (MQ=255)
                                                                                      cggcgTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCATTtttgcct             >  1:2748791/1‑100 (MQ=255)
                                                                                       ggcgTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTC‑‑GTAGGCATGATAAGCGGAGCGCATCAGGCATTtttgcctt            >  1:6083/1‑100 (MQ=255)
                                                                                         cgTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCATTtttgcctttg          >  2:636789/1‑100 (MQ=255)
                                                                                           ttAACGCCTTATCCGGCCTACAAAATCGTGTAAATTCAATACATTGCAGAATTTTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCATTtttgcctttg          <  2:2555109/98‑1 (MQ=255)
                                                                                            tAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCATTtttgcctttgcc        >  2:1710077/1‑99 (MQ=255)
                                                                                            tAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCATTTTTGCCTTTGCCa       <  2:1381428/100‑1 (MQ=255)
                                                                                              aCGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCATTTTTGCCTTTGCCATc     <  1:2420009/100‑1 (MQ=255)
                                                                                               cGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCATTTTTGCCTTTGCTATCa    >  1:2333888/1‑100 (MQ=255)
                                                                                                gCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCATTTTTGCCTTTGCCATCAg   <  1:1116001/100‑1 (MQ=255)
                                                                                                 ccTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCATTTTTTCCTTTGCCATCatt  >  2:3045457/1‑98 (MQ=255)
                                                                                                 ccTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCATTTTTGCCTTTGCCATCAGt  >  2:1744233/1‑100 (MQ=255)
                                                                                                   |                                                                                                     
TTGCTGCCGGATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCATTTTTGCCTTTGCCATCAGT  >  NC_000913/3705885‑3706081

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: