Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,706,028 T→C 15.8% intergenic (‑238/+70) dppB ← / ← dppA dipeptide/heme ABC transporter permease/dipeptide/heme ABC transporter periplasmic binding protein; dipeptide chemotaxis receptor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,706,0280TC15.8% 345.1 / 46.5 155intergenic (‑238/+70)dppB/dppAdipeptide/heme ABC transporter permease/dipeptide/heme ABC transporter periplasmic binding protein; dipeptide chemotaxis receptor
Reads supporting (aligned to +/- strand):  ref base T (74/51);  new base C (21/2);  total (102/53)
Fisher's exact test for biased strand distribution p-value = 3.64e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 159 total aligned reads displayed.

TACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCATTTTTGCCTTTGCCATCAGTCTTGTATGGCTTTTAATTATTCGATAGAGACGTTTTCGAAGTGA  >  NC_000913/3705931‑3706125
                                                                                                   |                                                                                                   
taCAGATTGTTGACAAAGTGCGCTTTGTTTATGCCTGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTTCAAATTCAATACATTGCAGAAtttt                                                                                                   >  2:117613/1‑100 (MQ=255)
taCAGATTGTTGACAAAGTGCGCTTTGTTTATCACGGATGCAGCGTTAACTCCTTATCCGGCCTACAAAATCGTGCAAATTCATTACATTGCAGAAtttt                                                                                                   <  2:85273/100‑1 (MQ=17)
taCAGATTGTTGAAAAAGTGCGCTTTGTTTATGCCGGACGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAAtttt                                                                                                   >  2:1871446/1‑100 (MQ=255)
  cAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTC‑‑g                                                                                               >  1:2857584/1‑100 (MQ=255)
  cAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTC‑‑g                                                                                               >  1:1702126/1‑100 (MQ=255)
  cAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTC‑‑g                                                                                               <  2:2293156/100‑1 (MQ=255)
   aGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTC‑‑Gt                                                                                              >  1:701618/1‑100 (MQ=255)
    gATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTC‑‑GTa                                                                                             <  2:371916/100‑1 (MQ=255)
      ttgttgACAAAGTGCGCTTTGTTTATGCCGGATGCTGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTC‑‑GTAgg                                                                                           <  1:1201207/100‑1 (MQ=255)
      ttgttgACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTC‑‑GTAgg                                                                                           <  2:546365/100‑1 (MQ=255)
        gttgACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGTTTTATCCTGTTTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTC‑‑GTAGGcc                                                                                         <  1:983872/100‑1 (MQ=18)
         ttgACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTC‑‑GTATGCCt                                                                                        >  2:1931382/1‑100 (MQ=255)
           gACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTC‑‑GTAGGCCTGa                                                                                      >  2:1712624/1‑100 (MQ=255)
            aCAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTC‑‑GTAGGCCTGAt                                                                                     <  2:2811126/100‑1 (MQ=255)
            aCAAAGTGCGCTTTGTTTATGCAGGATGCGGCGTTAAAGCCTTATCAGGCATACAAAATCGTGCAAATTCAATACATTGCAGAATTTTC‑‑GTAGGCCTGAt                                                                                     >  2:2140109/1‑100 (MQ=18)
               aaGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTC‑‑GTAGGCCTGATAAg                                                                                  <  1:2655613/100‑1 (MQ=255)
                aGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTC‑‑GTAGGCCTGATAAGc                                                                                 >  1:2827266/1‑100 (MQ=255)
                     gcTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTC‑‑GTAGGCCTGATAAGCGGAgc                                                                            <  2:459978/100‑1 (MQ=255)
                      cTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTAGAAAATCGTGCAAATTCAATACATTGCAGAATTTTC‑‑GTAGGCCTGATAAGCGGAgcg                                                                           <  2:1944875/100‑1 (MQ=255)
                      cTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTC‑‑GTAGGCCTGATAAGCGGAgc                                                                            <  1:405163/99‑1 (MQ=255)
                       tttGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTC‑‑GTAGGCCTGATAAGCGGAgcgc                                                                          >  1:746255/1‑100 (MQ=255)
                          gTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCTTGCAAATTCAATACATTGCAGAAATTTC‑‑GTAGGCCTGATAAGCGGAGCGCATc                                                                       >  1:1652348/1‑100 (MQ=255)
                          gTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGGATTTTC‑‑GTAGGCCTGATCAGCGGAGCGCATc                                                                       <  1:2044564/100‑1 (MQ=255)
                          gTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTC‑‑GTAGGCCTGATAAGCGGAGCGCATc                                                                       <  2:2326732/100‑1 (MQ=255)
                          gTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTC‑‑GTAGGCCTGATAAGCGGAGCGCATc                                                                       >  1:284379/1‑100 (MQ=255)
                           tttATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTC‑‑GTAGGCCTGATAAGCGGAGCGAATCa                                                                      >  1:577411/1‑100 (MQ=255)
                           tgtaggCCGGATAAGGCGTTTACGCCGCATCCGGCCTACAAAAGCGTGCAAATTCAATACATTGCATGGGCCAT‑‑GTAGGCCTGATAAGCGTAGCGCATCa                                                                      >  1:1815373/6‑100 (MQ=17)
                            gtaggCCGGATAAGGCGTTCACGCCGCATCCGGCCTACAAAAGCATGAAAATTCAATATATTGCAGGAGCTGC‑‑GTAGGCCTGATAAGCGTAGCGCATCAg                                                                     >  2:3020255/5‑100 (MQ=18)
                            gtaggCCGGATAAGGCGTTCACGCCGCATCCGGCCTACAAAACTTTGCAAATTCAATATATTGCAATCTCCGTGTAGGCCTGATAAGCGTAGCGCATCAg                                                                     <  1:2399970/96‑1 (MQ=18)
                             taggCCGGATAAGGCGTTCACTCCGCATCCGGCCTACAAAAGCACGCAAATTCAATATATTGCAGAGATTGC‑‑GTAGGCCTGATAAGCGTAGCGCATCAg                                                                     >  2:2521256/4‑99 (MQ=14)
                             taggCCGGATAAGGCGTTCACGCCGCATCCGGCCTACAAAATTGTGTAAATTCAATAAATTGATCGATCCAC‑‑GTAGGCCTGATAAGCGTAGCGCATCAgg                                                                    >  2:3026071/4‑100 (MQ=21)
                             tATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATCCAATACATTGCAGAATTTTC‑‑GTAGGCCTGATAAGCGGAGCGCATAAgg                                                                    >  2:1289242/1‑100 (MQ=255)
                              aggCCGGATAAGGCGTTTACGCCGCATCCGGCCTACAAAAGCGTGCAAATTCAATACATTGCATGGGCCAT‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGc                                                                   >  1:412280/3‑100 (MQ=17)
                              aggCCGGATAAGGCGTTCACGCCGCATCCGGCCTACAAAATCATGAAAATTCAATACATTGCAAGATTTTC‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGc                                                                   >  1:396157/3‑100 (MQ=17)
                               ggccagataaggcgTTCACGCCGCATCCGGCCTACAAAACTTTGCAAATTCAATATATTGCAATCTCCGTGTAGGCCTGACAAGCGTAGCGCATCAGGCa                                                                  >  2:2877078/11‑100 (MQ=18)
                               ggCCGGATAAGGCGTTTACGCCGCATCCGGCCTACAAAAGCATGCAAATTCAATATATTGCAGAGTTCAC‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGCa                                                                  >  1:85464/2‑100 (MQ=14)
                               ggCCGGATAAGGCGTTCACGCCGCATCCGGCCTACAAAGTCGTATAAATTCAATGTATTGCAGTTTATTG‑‑GTAGGCCTGATAAGCGCAGCGCATCAGGCa                                                                  >  1:509184/2‑100 (MQ=21)
                               ggCCGGATAAGGCGTTCACGCCGCATCCGGCCTACAAAATTGTGTAAATTCAATAAATTGATCGATCCAC‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGCa                                                                  >  2:1106044/2‑100 (MQ=21)
                               ggCCGGATAAGGCGTTCACGCCGCATCCGGCCTACAAAAACCTGCAAATTCAATATATTGCAGGATACGT‑‑ATAGGCCTGATAAGCGTAGCGCATCAGGCa                                                                  >  1:321438/2‑100 (MQ=17)
                                gCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCAt                                                                 >  1:370551/1‑100 (MQ=255)
                                gCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCAt                                                                 >  1:1233024/1‑100 (MQ=255)
                                gCCGGATAAGGCGTTCACGCCGCATCCGGCCTACAAAACTTTGCAAATTCAATATATTGCAATCTCCGTGTAGGCCTGATAAGCGTAGCGCATCAGGCaa                                                                 >  1:2262768/1‑99 (MQ=18)
                                 ccggataaggcgTTCACGCCGCATCCGGCCTACAAGATCGTGCAAATTCAATATATTGCATGAGCCAC‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGCagt                                                                >  1:146806/9‑98 (MQ=17)
                                 ccggataaggcgTTCACGCCGCATCCGGCCTACAAAACTTTGCAAATTCAATATATTGCAATCTCCGTGTAGGCCTGATAAGCGTAGCGCATCAGGCaat                                                                >  2:257069/9‑98 (MQ=18)
                                 ccGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCAtt                                                                >  1:2028258/1‑100 (MQ=255)
                                 ccGGATCCGGCGTTAACGCCTTATCCAGCCTACAAAATTGTGCAAAATCAATTGATTGCACAGCTAGC‑‑GTAGGCCTGATGCGCTACGCTCATCAGGc                                                                   <  2:1483766/97‑1 (MQ=18)
                                  cggataaggcgTTCACGCCGCATCCGGCCTACAAAATCGCTTAAATTCAATATATTGCACCATCCTC‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGCaat                                                                >  2:1357700/8‑97 (MQ=18)
                                   ggataaggcgTTTACGCCGCATCCGGCCTACAAATTCTGCAAATTCAACAAATTGCGAATCCCTT‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGCAtttgc                                                             >  1:2576818/7‑98 (MQ=18)
                                   ggataaggcgTTTACGCCGCATCCGGCCTACAAAAGCATGCAAATTCAATATATTGCAGAGTTCAC‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGCAgttt                                                              >  2:1008095/7‑100 (MQ=17)
                                   ggataaggcgTTCACGCCGCGTCCGGCCTACAAAGTCGTATAAATTCAATGTATTGCAGGTTATTG‑‑GTAGGCCTGATAAGCGCAGCGCATCAGGCaatct                                                              >  1:2580452/7‑96 (MQ=21)
                                   ggataaggcgTTCACGCCGCATCCGGCCTACAAGATCGTGCAAATTCAATATATTGCATGAGCCAC‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGCAgttt                                                              >  1:131475/7‑100 (MQ=17)
                                   ggataaggcgTTCACGCCGCATCCGGCCTACAAAGTCGTATAAATTCAATGTATTGCAGTTTATTG‑‑GTAGGCCTGATAAGCGCAGCGCATCAGGCaatct                                                              >  2:756655/7‑96 (MQ=25)
                                   ggataaggcgTTCACGCCGCATCCGGCCTACAAAAGCATGAAAATTCAATATATTGCAGGAGCTGC‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGCAgtt                                                               >  1:3034549/7‑99 (MQ=17)
                                   ggataaggcgTTCACGCCGCATCCGGCCTACAAAACCTTGCTAATTCAATATATTGCAGGGACTAT‑‑GTAGGCCTGATAAGCATAGCGCATCAGGCagctt                                                              >  2:1239319/7‑96 (MQ=21)
                                   ggataaggcgTTCACGCCGCATCCGGCCTACAAATCATGCAAATCCAAAATATTGCAGGAGTTAT‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGCAtttat                                                             >  1:1066030/7‑98 (MQ=21)
                                   ggataaggcgTTCACGCCGCATCCGGCCCACAAAGTCTTGCAAAATCAATAAATTGCAGGAACGAT‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGCaacta                                                              >  2:2269240/7‑96 (MQ=21)
                                   ggataaggcgTTCACGCCGCATCCGGCATACAAATCATGCAAATCCAAAATATTGCAGGAGATAT‑‑GTAGGCCTGATAAGCGTAGCGCATCAGACAttta                                                              >  1:2756742/7‑98 (MQ=18)
                                   ggATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCAtttt                                                              <  1:2969638/100‑1 (MQ=255)
                                    gataaggcgTTTACGCCGCATCCGGCCTACAAAAGCATGCAAATTCAATATATTGCAGAGTTCAC‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGCAgtttt                                                             >  2:2192871/6‑100 (MQ=17)
                                     ataaggcgTTCACGCCGCATCCGGCCTACAAGATCGTGCAAATTCAATATATTGCATGAGCCAC‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGCAGTtttg                                                            <  2:146804/96‑1 (MQ=17)
                                     aTGGGGCGTTATCGCTTTATAGGGCGAAAAAAATCGTGGAAATTCACTACATTGCAGAATTTTC‑‑GGAGGGCTAATAAGTGGAGCGCATCAGGCATTtttg                                                            <  2:1974961/100‑1 (MQ=16)
                                       gcggcgTTAACGCCTTATCCAGCCTACAAAATAGTACAAAATCAATACATTGCAGAATTTTC‑‑GTAGGCCTGATAAGCGGAGAGCATCAGGCATTtttgcc                                                          >  2:704377/1‑100 (MQ=18)
                                         ggcgTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTC‑‑GTAGGCATGATAAGCGGAGCGCATCAGGCATTtttgcctt                                                        >  1:6083/1‑100 (MQ=255)
                                             ttAACGCCTTATCCGGCCTACAAAATCGTGTAAATTCAATACATTGCAGAATTTTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCATTtttgcctttg                                                      <  2:2555109/98‑1 (MQ=255)
                                              tAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCATTtttgcctttgcc                                                    >  2:1710077/1‑99 (MQ=255)
                                              tAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCATTTTTGCCTTTGCCa                                                   <  2:1381428/100‑1 (MQ=255)
                                               aaCGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCATTTTTGCCTTTGCCAt                                                  >  1:2196911/1‑100 (MQ=255)
                                                aCGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCATTTTTGCCTTTGCCATc                                                 <  1:2420009/100‑1 (MQ=255)
                                                  gCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCATTTTTGCCTTTGCCATCAg                                               <  1:1116001/100‑1 (MQ=255)
                                                       aTCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCATTTTTGCCTTTGCCATCAGTCTTg                                          <  1:2760442/100‑1 (MQ=255)
                                                         ccGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCATTTTTGCCTTTGCCATCAGTCTTGTa                                        >  1:1980526/1‑100 (MQ=255)
                                                          cGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCATTTTTGCCTTTGCCATCAGTCTTGTAt                                       >  1:464485/1‑100 (MQ=255)
                                                            gCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTC‑‑TTAGGCCTGATAAGCGTAGCGCATCATGCATTTTTGCCTTTGCCATCAGTCtt                                           <  2:2656694/94‑1 (MQ=255)
                                                             ccTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCATTTTTGCCTTTGCCATCAGTCTTGTATGGc                                    <  2:1831134/100‑1 (MQ=255)
                                                             ccTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCATTTTTGCCTTTGCCATCAGTCTTGTATGGc                                    <  2:1626755/100‑1 (MQ=255)
                                                             ccTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCATTTTTGCCTTTGCCATCAGTCTTGTATGGc                                    >  2:2691448/1‑100 (MQ=255)
                                                               tACAAAATCGTGCAAATTCAATACATTGCAGAATTTTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCATTTTTGCCTTTGCCATCAGTCTTGTATGGCtt                                  >  2:671196/1‑100 (MQ=255)
                                                                  aaaaTCGTGCAAATTCAATACATTGCAGAATTTTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCATTTTTGCCTTTGCCATCAGTCTTGTATGGCTTTTa                               <  2:1227770/100‑1 (MQ=255)
                                                                    aaTCGTGCAAATTCAATACATTGCAGAATTTTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCATTTTTGCCTTTGCCATCAGTCTTGTATGGCTTTTAat                             <  2:496477/100‑1 (MQ=255)
                                                                     aTCGTGCAAATTCAATACATTGCAGAATTTTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCATTTTTGCCTTTGCCATCAGTCTTGTATGGCTTTTAatt                            >  2:919909/1‑100 (MQ=255)
                                                                      tCGTGCAAATTCAATACATTGCAGAATTTTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCATTTTTGCCTTTGCCATCAGTCTTGTATGGCTTTTAatta                           <  2:796912/100‑1 (MQ=255)
                                                                      tCGTGCAAATTCAATACATTGCAGAATTTTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCATTTTTGCCTTTGCCATCAGTCTTGTATGGCTTTTAatta                           <  2:388500/100‑1 (MQ=255)
                                                                       cGTGCAAATTCAATACATTGCAGAATTTTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCATTTTTGCCTTTGCCATCAGTCTTGTATGGCTTTTAattat                          >  1:1187900/1‑100 (MQ=255)
                                                                        gTGCAAATTCAATACATTGCAGAATTTTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCATTTTTGCCTTTGCCATCAGTCTTGTATGGCTTTTAattatt                         >  2:2082608/1‑100 (MQ=255)
                                                                        gTGCAAATTCAATACATTGCAGAATTTTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCATTTTTGCCTTTGCCATCAGTCTTGTATGGCTTTTAattatt                         >  1:1933076/1‑100 (MQ=255)
                                                                         tGCAAATTCAATACATTGCAGAATTTTC‑‑GTAGGCCTGATAAGCGGAGCTCATCAGGCATTTTTGCCTTTGCCATCAGTCTTGTATGGCTTTTAATTATTc                        <  1:997315/100‑1 (MQ=255)
                                                                         tGCAAATTCAATACATTGCAGAATTTTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCATTTTTGCCTTTGCCATCAGTCTTGTATGGCTTTTAATTATTc                        <  1:1549065/100‑1 (MQ=255)
                                                                          gCAAATTCAATACATTGCAGAATTTTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCATTTTTGCCTTTGCCATCAGTCTTGTATGGCTTTTAATTATTCg                       >  2:2394884/1‑100 (MQ=255)
                                                                            aaaTTCAATACATTGCAGAATTTTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCATTTTTTTCTTTTTCTTCAGTCTTGTATGGCGTTTAATTATTTGAt                     >  2:2067293/1‑100 (MQ=21)
                                                                               ttCAATACATTGCAGAATTTTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCATTTTTGCCTTTGCCATCAGTCTTGTATGGCTTTTAATTATTCGATaga                  <  2:1690613/100‑1 (MQ=255)
                                                                                tCAATACATTGCAGAATTTTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCATTTTTGCCTTTGCCATCAGTCTTGTATGGCTTTTAATTATTCGATagag                 >  1:962990/1‑100 (MQ=255)
                                                                                tCAATACATTGCAGAATTTTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCATTTTTGCCTTTGCCATCAGTCTTATATGGCTTTTAATTATTCGATagag                 >  1:632627/1‑100 (MQ=255)
                                                                                 cAATACATTGCAGAATTTTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCATTTTTGCCTTTGCCATCAGTCTTGTATGGCTTTTAATTATTCGATAgaga                <  2:1117033/100‑1 (MQ=255)
                                                                                   aTACATTGCAGAATTTTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCATTTTTGCCTTTGCCATCAGTCTTGTATGGCTTTTAATTATTCGATAGAGACg              >  2:1666298/1‑100 (MQ=255)
                                                                                        ttGCAGAATTTTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCATTTTTGCCTTTGCCATCAGTCTTATATGGCTTTTAATTATTCGATAGAGACGTTTTc         >  1:259594/1‑100 (MQ=255)
                                                                                          gCAGAATTTTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCATTTTTGCCTTTGCCATCAGTCTTGTATGGCTTTTAATTATTCGATAGAGACGTTTTCGa       <  1:260192/100‑1 (MQ=255)
                                                                                            aGAATTTTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCATTTTTGCCTTTGCCATCAGTCTTGTATGGCTTTTAATTATTCGATAGAGACGTTTTCGAAg     <  2:2391597/100‑1 (MQ=255)
                                                                                              gAATTTTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCATTTTTGCCTTTGCCATCAGTCTTGTATGGCTTTTAATTATTCGATAGAGACGTTTTCGAAGt    <  2:2233571/100‑1 (MQ=255)
                                                                                              gAATTTTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCATTTTTGCCTTTGCCATCAGTCTTGTATGGCTTTTAATTATTCGATAGAGACGTTTTCGAAGt    >  1:1545261/1‑100 (MQ=255)
                                                                                                aTTTTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCATTTTTGCCTTTGCCATCAGTCTTGTATGGCTTTTAATTATTCGATAGAGACGTTTTCGAAGTGa  >  1:2223029/1‑100 (MQ=255)
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TACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCATTTTTGCCTTTGCCATCAGTCTTGTATGGCTTTTAATTATTCGATAGAGACGTTTTCGAAGTGA  >  NC_000913/3705931‑3706125

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: