BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	53,012	A→G	100%	G135G (GGT→GGC)	pdxA ←	4‑hydroxy‑L‑threonine phosphate dehydrogenase, NAD‑dependent

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	53,012	0	A	G	100.0%	391.4 / NA	113	G135G (GGT→GGC)	pdxA	4‑hydroxy‑L‑threonine phosphate dehydrogenase, NAD‑dependent
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (50/63); total (50/63)

Only 100 of 113 total aligned reads displayed.

GTAAATGCGTCGTTGCCAGCGCCACGCGAAGTTCTTCGGTCGCCAGCATCATCACCACCTTTTTCGCCTGCGAACGCTCTTCGAAAAACTCGGTATGACCGGTAAAAGGAATGCCAGCGTCGTTAATAACGCCTTTATGCACCGGACCTGTGATCAGCGCGGCAAATTCGCCGTTCAGACAACCATCGCACGCT  >  NC_000913/52915‑53108

gTAAATGCGTCGTTGCCAGCGCCACGCGAAGTTCTTCGGTCGCCAGCATCATCACCACCTTTTTCGCCTGCGAACGCTCTTCGAAAAACTCGGTATGGcc                                                                                                <  1:181856/100‑1 (MQ=255)

  aaaTGCGTCGTTGCCAGCGCCACGCGAAGTTCTTCGGTCGCCAGCATCATCACCACCTTTTTCGCCTGCGAACGCTCTTCGAAAAACTCGGTATGGCCgg                                                                                              <  2:1833737/100‑1 (MQ=255)

   aaTGCGTCGTTGCCAGCGCCACGCGAAGTTCTTCGGTCGCCAGCATCATCACCACCTTTTTCGCCTGCGAACGCTCTTCGAAAAACTCGGTATGGCCGGt                                                                                             <  1:1309559/100‑1 (MQ=255)

   aaTGCGTCGTTGCCAGCGCCACGCGAAGTTCTTCGGTCGCCAGCATCATCACCACCTTTTTCGCCTGCGAACGCTCTTCGAAAAACTCGGTATGGCCGGt                                                                                             <  1:495610/100‑1 (MQ=255)

     tGCGTCGTTGCCAGCGCCACGCGAAGTTCTTCGGTCGCCAGCATCATCACCACCTTTTTCGCCTGCGAACGCTCTTCGAAAAACTCGGTATGGCCGGTaa                                                                                           >  2:2151718/1‑100 (MQ=255)

       cgtcgtTGCCAGCGCCACGCGAAGTTCTTCGGTCGCCAGCATCATCACCACCTTTTTCGCCTGCGAACGCTCTTCGAAAAACTCGGTATGGCCGGTaaaa                                                                                         >  1:1110989/1‑100 (MQ=255)

        gtcgtTGCCAGCGCCACGCGAAGTTCTTCGGTCGCCAGCATCATCACCACCTTTTTCGCCTGCGAACGCTCTTCGAAAAACTCGGTATGGCCGGTAAAAg                                                                                        <  2:2210639/100‑1 (MQ=255)

         tcgtTGCCAGCGCCACGCGAAGTTCTTCGGTCGCCAGCATCATCACCACCTTTTTCGCCTGCGAACGCTCTTCGAAAAACTCGGTATGGCCGGTAAAAgg                                                                                       <  2:950648/100‑1 (MQ=255)

          cgtTGCCAGCGCCACGCGAAGTTTTTCGGTCGCCAGCATCATCACCACCTTTTTCGCCTGCGAACGCTCTTCGAAAAACTCGGTATGGCCGGTAAAAGGa                                                                                      <  1:1310239/100‑1 (MQ=255)

               ccAGCGCCACGCGAAGTTCTTCGGTCGCCAGCATCATCACCACACTTTTCGCCTGCGAACGCTCTTCGAAAAACTCGGTATGGCCGGTAAAAGGAATGcc                                                                                 >  2:2192828/1‑100 (MQ=255)

                cAGCGCCACGCGAAGTTCTTCGGTCGCCAGCATCATCACCACCTTTTTCGCCTGCGAACGCTCTTCGAAAAACTCGGTATGGCCGGTAAAAGGAATGCCa                                                                                >  1:43489/1‑100 (MQ=255)

                      cACGCGAAGTCCTTCGGTCGCCAGCATCATCACCACCTTTTTCGCCTGCGAACGCTCTTCGAAAAATTCGGTATGGCCGGTAAAAGGAATGCCAGcgtcg                                                                          <  2:1992914/100‑1 (MQ=255)

                        cgcgAAGTTCTTCGGTCGCCAGCATCATCACCACCTTTTTCGCCTGCGAACGCTCTTCGAAAAACTCGGTATGGCCGGTAAAAGGAATGCCAGCGTCGtt                                                                        >  2:1147148/1‑100 (MQ=255)

                        cgcgAAGTTCTTCGGTCGCCAGCATCATCACCACCTTTTTCGCCTGCGAACGCTCTTCGAAAAACTCGGTATGGCCGGTAAAAGGAATGCCAGCGTCGtt                                                                        <  2:218684/100‑1 (MQ=255)

                         gcgAAGTTCTTCGGTCGCCAGCATCATCTTCACCTTTTTTGCATGCGAACGCTCTTCGAAAAACTCGGTATGGCCGGTAAAAGGAATGCCAGCGTCGTta                                                                       <  1:25559/100‑1 (MQ=255)

                         gcgAAGTTCTTCGGTCGCCAGCATCATCACCACCTTTTTCGCCTGCGAACGCTCTTCGAAAAACTCGGTATGGCCGGTAAAAGGAATGCCAGCGTCGTta                                                                       <  1:2567720/100‑1 (MQ=255)

                         gcgAAGTTCTTCGGTCGCCAGCATCATCACCACCTTGTTCGCCTGCGAACGCTCTTCGAAAAACTCGGTATGGCCGGTAAAAGGAATGCCAGCGTCGTta                                                                       <  1:2132695/100‑1 (MQ=255)

                          cgAAGTTCTTCGGTCGCCAGCATCATCACCACCTTTTTCGCCTGCGAACGCTCTTCGAAAAACTCGGTATGGCCGGTAAAAGGAATGCCAGCGTCGTtaa                                                                      >  2:1662139/1‑100 (MQ=255)

                          cgAAGTTCTTCGGTCGCCAGCATCATCACCACCTTTTTCGCCTGCGAACGCTCTTCGAAAAACTCGGTATGGCCGGTAAAAGGAATGCCAGCGTCGTtaa                                                                      >  2:462173/1‑100 (MQ=255)

                             aGTTCTTCGGTCGCCAGCATCATCACCACCTTTTTCGCATGCGAACGCTCTTCGAAAAACTCGGTATGGCCGGTAAAAGGAATACCAGCGTCGTtaataa                                                                   >  2:1347060/1‑100 (MQ=255)

                              gTTCTTCGGTCGCCAGCATCATCACCACCTTTTTCGCCTGCGAACGCTCTTCGAAAAACTCGGTATGGCCGGTAAAAGGAATGCCAGCGTCGTTAATAAc                                                                  <  2:632870/100‑1 (MQ=255)

                              gTTCTTCGGTCGCCAGCATCATCACCACCTTTTTCGCCTGCGAACGCTCTTCGAAAAACTCGGTATGGCCGGTAAAAAGAATGCCAGCGTCGTtaataa                                                                   >  1:1000059/1‑99 (MQ=255)

                                tcttcGGTCGCTAGCATCATCACCACCTTTTTCGCCTGCGAACGCTCTTCGAAAAGCTCGGTATGGCCGGTAAAAGGAATGCCAGCGTCGTTAATAACGc                                                                >  2:368703/1‑100 (MQ=255)

                                tcttcGGTCGCCAGCATCATCACCACCTTTTTTTTTTTTTTTCGCACTTCGAAAAACTCGGTATGGCCGTTAAAAGGAATGCCAGCGTCGTTATTCACGc                                                                >  2:1296439/1‑100 (MQ=255)

                                tcttcGGTCGCCAGCATCATCACCACCTTTTTCGCCTGCGAACGCTCTTCGAAAAACTCGGTATGGCCGGTAAAAGGAATGCCAGCGTCGTTAATAACGc                                                                <  1:672161/100‑1 (MQ=255)

                                 cttcGGTCGCCAGCATCATCACCACCTTTTTCGCCTGCGAACGCTCTTCGAAAAACTCGGTATGGCCGGTAAAAGGAATGCCAGCGTCGTTAATAACGcc                                                               <  1:2489026/100‑1 (MQ=255)

                                 cttcGGTCGCCAGCATCATCACCACCTTTTTCGCCTGCGAACGCGCTTCGAAAAACTCGGTATGGCCGGTAAAAGGAATGCCAGCGTCGTTAATAACGcc                                                               <  2:1922154/100‑1 (MQ=255)

                                  ttcGGTCGCCAGCATCATCACCACCTTTTTCGCCTGCGAACGCTCTTCGAAAAACTCGGTATGGCCGGTAAAAGGAATGCCAGCGTCGTTAATAACGCCt                                                              >  2:2899642/1‑100 (MQ=255)

                                  ttcGGTCGCCAGCATCATCACCACCTTTTTCGCCTGCGAACGCTCTTCGAAAAACTCGGTATGGCCGGTAAAAGGAATGCCAGCGTCGTTAATAACGCCt                                                              <  1:779870/100‑1 (MQ=255)

                                   tcGGTCGCTAGCATCATCACCACCTTTTTCGCCTGCGAACGCTCTTCGAAAAGCTCGGTATGGCCGGTAAAAGGAATGCCAGCGTCGTTAATAACGCCtt                                                             <  1:368706/100‑1 (MQ=255)

                                    cGGTCGCCAGCATCATCACCACCTTTTTCGCCTGCGAACGCTCTTCGAAAAACTCGGTATGGCCGGTAAAAGGAATGCCAGCGTCGTTAATAACGCCttt                                                            >  1:1665919/1‑100 (MQ=255)

                                    cGGTCGCCAGCATCATCACCACCTTTTTCGCCTGCGAACGCTCTTCGAAAAACTCGGTATGGCCGGTAAAAGGAATGCCAGCGTCGTTAATAACGCCttt                                                            >  1:2195855/1‑100 (MQ=255)

                                    cGGTCGCCAGCATCATCACCACCTTTTTCGCCTGCGAACGCTCTTCGAAAAACTCGGTATGGCCGGTAAAAGGAATGCCAGCGTCGTTAATAACGCCttt                                                            <  2:2494605/100‑1 (MQ=255)

                                    cGGTCGCCAGCATCATCACCACCTTTTTCGCCTGCGAACGCTCTTCGAAAAACTCGGTATGGCCGGTAAAAGGAATGCCAGCGTCGTTAATAACGCCttt                                                            >  2:2587169/1‑100 (MQ=255)

                                     ggTCGCCAGCATCATCACCACCTTTTTCGCCTGCGAACGCTCTTCGAAAAACTCGGAATGGCCGGTAAAAGGAATGCCAGCGTCGTTAATAACGCCTTTa                                                           >  1:294921/1‑100 (MQ=255)

                                      gTCGCCAGCATCATCACCACCTTTTTCGCCTGCGAACGCTCTTCGAAAAACTCGGTATGGCCGGTAAAAGGAATGCCAGCGTCGTTAATAACGCCTTTAt                                                          >  2:2697892/1‑100 (MQ=255)

                                      gTCGCCAGCATCATCACCACCTTTTTCGCCTGCGAACGCTCTTCGAAAAACTCGGTATGGCCGGTAAAAGGAATGCCAGCGTCGTTAATAACGCCTTTAt                                                          <  1:1073197/100‑1 (MQ=255)

                                        cGCCATCATCATCACCACCTTTTTCGCCTGCGAACTCTCTTCGAAAAACTCGGTATGGCCGGTAAAAGGAATGCCAGCGTCGTTAATAACGCCTTTATGc                                                        <  1:1763454/100‑1 (MQ=255)

                                        cGCCAGCATCATCACCACCTTTTTCGCCTGCGAACGCTCTTCGAAAAACTCGGTATGGCCGGTAAAAGGAATGCCAGCGTCGTTAATAACGCCTTTATGc                                                        <  1:529208/100‑1 (MQ=255)

                                         gCCAGCATCATCACCACCTTTTTCGCCTGCGAACGCTCTTCGAAAAACTCGGTATGGCCGGTAAAAGGAATGCCAGCGTCGTTAATAACGCCTTTATGCa                                                       >  1:2989443/1‑100 (MQ=255)

                                         gCCAGCATCATCACCACCTTTTTCGCCTGCGAACGCTCTTCGAAAAACTCGGTATGGCCAGAAAAAAGAATGACAGCGTAGTTAATAAAACCTTTATGCa                                                       >  1:392952/1‑100 (MQ=255)

                                             gCATCATCACCACCTTTTTCGCCTGCGAACGCTCTTCGAAAAACTCGGTATGGCCGGTAAAAGGAATGCCAGCGTCGTTAATAACGCCTTTATGCACCgg                                                   <  1:1177910/100‑1 (MQ=255)

                                             gCATCATCACCACCTTTTTCGCCTGCGAACGCTCTTCGAAAAACTCGGTATGGCCGGTAAAAGGAATGCCAGCGTCGTTAATAACGCCTTTATGCACCgg                                                   <  2:43489/100‑1 (MQ=255)

                                                tcatcaCCACCTTTTTCGCCTGCGAACGCTCTTCGAAAAACTCGGTATGGCCGGTAAAAGGAATGCCAGCGTCGTTAATAACGCCTTTATGCACCGGAcc                                                <  1:1469090/100‑1 (MQ=255)

                                                tcatcaCCACCTTTTTCGCCTGCGAACGCTCTTCGAAAAACTCGGTATGGCCGGTAAAAGGAATGCCAGCGTCGTTAATAACGCCTTTATGCACCGGAcc                                                <  1:75283/100‑1 (MQ=255)

                                                 catcaCCACCTTTTTCGCCTGCGAACGCTCTTCGAAAAACTCGGTATGGCCGGTAAAAGGAATGCCAGCGTCGTTAATAACGCCTTTATGCACCGGACCt                                               >  2:2836252/1‑100 (MQ=255)

                                                   tcaCCACCTTTTTCGCCTGCGAACGCTCTTCGAAAAACTCGGTATGGCCGGTAAAAGGAATGCCAGCGTCGTTAATAACGCCTTTATGCACCGGACCtgt                                             <  2:2768221/100‑1 (MQ=255)

                                                   tcaCCACCTTTTTCGCCTGCGAACGCTCTTCGAAAAACTCGGTATGGCCGGTAAAAGGAATGCCAGCGTCGTTAATAACGCCTTTATGCACCGGACCtgt                                             >  2:560740/1‑100 (MQ=255)

                                                    caccacCTTTTTCGCCTGCGAACGCTCTTCGAAAAACTCGGTATGGCCGGTAAAAGGAATGCCAGCGTCGTTAATAACGCCTTTATGCACCGGACCtgt                                             <  2:146331/99‑1 (MQ=255)

                                                      ccaccTTTTTCGCCTGCGAACGCTCTTCGAAAAACTCGGTATGGCCGGTAAAAGGAATGCCAGCGTCGTTAATAACGCCTTTATGCACCGGACCTGTGAt                                          <  2:3005806/100‑1 (MQ=255)

                                                      ccaccTTTTTCGCCTGCGAACGCTCTTCGAAAAACTCGGTATGGCCGGTAAAAGGAATGCCAGCGTCGTTAATAACGCCTTTATGCACCGGACCTGTGAt                                          <  2:3001777/100‑1 (MQ=255)

                                                      ccaccTTTTTCGCCTGCGAACGCTCTTCGAAAAACTCGGTATGGCCGGTAAAAGGAATGCCAGCGTCGTTAATAACGCCTTTATGCACCGGACCTGTGAt                                          <  2:1460775/100‑1 (MQ=255)

                                                      ccaccTTTTTCGCCTGCGAACGCTCTTCGAAAAACTCGGTATGGCCGGTAAAAGGAATGCCAGCGTCGTTAATAACGCCTTTATGCACCGGACCTGTGAt                                          <  2:3000741/100‑1 (MQ=255)

                                                       caccTTTTTCGCCTGCGAACGCTCTTCGAAAAACTCGGTATGGCCGGTAAAAGGAATGCCAGCGTCGTTAATAACGCCTTTATGCACCGGACCTGTGATc                                         <  2:321203/100‑1 (MQ=255)

                                                       caccTTTTTCGCCTGCGAACGCTCTTCGAAAAACTCGGTATGGCCGGTAAAAGGAATGCCAGCGTCGTTAATAACGCCTTTATGCACCGGACCTGTGATc                                         <  2:588894/100‑1 (MQ=255)

                                                       caccTTTTTCGCCTGCGAACGCTCTTCGAAAAACTCGGTATGGCCGGTAAAAGAAATGCCAACGTCGTTAATAACGCCTTTATGCACCGGACCTGTGATc                                         >  1:947976/1‑100 (MQ=255)

                                                        accTTTTTCGCCTGCGAACGCTCTTCGAAAAACTCGGTATGGCCGGTAAAAGGAATGCCAGCGTCGTTAATAACGCCTTTATGCACCGGACCTGTGATCa                                        <  2:2197484/100‑1 (MQ=255)

                                                         ccTTTTTCGCCTGCGAACGCTCTTCGAAAAACTCGGTATGGCCGGTAAAAGGAATGCCAGCGTCGTTAATAACGCCTTTATGCACCGGACCTGTGATCAg                                       >  1:2836644/1‑100 (MQ=255)

                                                         ccTTTTTCGCCTGCGAACGCTCTTCGAAAAACTCGGTATGGCCGGTAAAAGGAATGCCAGCGTCGTTAATAACGCCTTTATGCACCGGACCTGTGATCAg                                       >  2:130483/1‑100 (MQ=255)

                                                            ttttCGCCTGCGAACGCTCTTCGAAAAACTCGGTATGGCCGGTAAAAGGAATGCCAGCGTCGTTAATAACGCCTTTATGCACCGGACCTGTGATCAgcgc                                    <  2:1100530/100‑1 (MQ=255)

                                                            ttttCGCCTGCGAACGCTCTTCGAAAAACTCGGTATGGCCGGTAAAAGGAATGCCAGCGTCGTTAATAACGCCTTTATGCACCGGACCTGTGATCAgcgc                                    >  1:1441536/1‑100 (MQ=255)

                                                              ttCGCCTGCGAACGCTCTTCGAAAAACTCGGTATGGCCGGTAAAAGGAATGCCAGCGTCGTTAATAACGCCTTTATGCACCGGACCTGTGATCAGCGCgg                                  <  1:168148/100‑1 (MQ=255)

                                                               tcgaccgCGAACGCTCTTCGAAAAACACGGTATGGCCGGAAAAAGGAATGCCAGCGTCGTTAATAACGCCTGTATGAACCGGACCAGGGATCa                                        >  2:2735976/7‑93 (MQ=255)

                                                               tagCCTGCGAACGCTCTTCGAAAAACTCGGTATGGCCGGTAACAGGAATGCCAGCGTCGTTAATAACGCCTTTATGCACCGGACCTGTGATCAGCGCGGc                                 <  2:186211/98‑1 (MQ=255)

                                                               tCGCCGGCGAACGCTCTTCGAAAAACTCGGTATGGCCGGTAAAAGGAATGCCAGCGTCGTTAATAACGCCTTTATGCACCGGACCTGTGATCAGCGCGGc                                 <  2:200803/100‑1 (MQ=255)

                                                                cGCCTGCGAACGCTCTTCGAAAAACTCGGTATGGCCGGTAAAAGGAATGCCAGCGTCGTTAATAACGCCTTTATGCACCGGACCTGTGATCAGCGCGGCa                                <  1:2937086/100‑1 (MQ=255)

                                                                 gCCTGCGAACGCTCTTCGAAAAACTCGGTATGGCCGGTAAAAGGAATGCCAGCGTCGTTAATAACGCCTTTATGCACCGGACCTGTGATCAGCGCGGCaa                               >  2:2201597/1‑100 (MQ=255)

                                                                  ccTGCGAACGCTCTTCGAAAAACTCGGTATGGCCGGTAAAAGGAATGCCAGCGTCGTTAATAACGCCTTTATGCACCGGACCTGTGATCAGCGCGGCaaa                              <  2:852707/100‑1 (MQ=255)

                                                                  ccTGCGAACGCTCTTCGAAAAACTCGGTATGGCCGGTAAAAGGAATGCCAGCGTCGTTAATAACGCCTTTATGCACCGAACCTGTGATCAGCGCGGCaaa                              >  1:2298634/1‑100 (MQ=255)

                                                                   cTGCGAACGCTCTTCGAAAAACTCGGTATGGCCGGTAAAAGGAATGCCAGCGTCGTTAATAACGCCTTTATGCACCGGACCTGTGATCAGCGCGGCAAAt                             <  2:2108043/100‑1 (MQ=255)

                                                                   cTGCGAACGCTCTTCGAAAAACTCGGTATGGCCGGTAAAAGGAATGCCAGCGTCGTTAATAACGCCTTTATGCACCGGACCTGTGATCAGCGCGGCAAAt                             <  2:1588970/100‑1 (MQ=255)

                                                                     gCGAACGCTCTTCGAAAAACTCGGTATGGCCGGTAAAAGGAATGCCAGCGTCGTTAATAACGCCTTTATGCACCGGACCTGTGATCAGCGCGGCAAATTc                           >  2:2353710/1‑100 (MQ=255)

                                                                     gCGAACGCTCTTCGAAAAACTCGGTATGGCCGGTAAAAGGAATGCCAGCGTCGTTAATAACGCCTTTATGCACCGGACCTGTGATCAGCGCGGCAAATTc                           >  2:2498462/1‑100 (MQ=255)

                                                                     gCGAACGCTCTTCGAAAAACTCGGTATGGCCGGTAAAAGGAATGCCAGCGTCGTTAATAACGCCTTTATGCACCGGACCTGTGATCAGCGCGGCAAATTc                           >  2:1120952/1‑100 (MQ=255)

                                                                      cGAACGCTCTTCGAAAAACTCGGTATGGCCGGTAAAAGGAATGCCAGCGTCGTTAATAACGCCTTTATGCACCGGACCTGTGATCAGCGCGGCAAATTCg                          <  2:868239/100‑1 (MQ=255)

                                                                        aaCGCTCTTCGAAAAACTCGGTATGGCTGTTAAATTGTATGTCAGCGTCGTTAATAACGCCTATATGCACCGGACCTGTGATCAGCGCGGCAAATTCg                          <  1:64692/98‑1 (MQ=255)

                                                                          cGCTCTTCGAAAAACTCGGTATGGCCGGTAAAAGGAATGCCAGCGTCGTTAATAACGCCTTTATGCACCGGACCTGTGATCAGCGCGGCAAATTCGCCg                       >  2:707197/1‑99 (MQ=255)

                                                                          cGCTCTTCGAAAAACTCGGTATGGCCGGTAAAAGGAATGCCAGCGTCGTTAATAACGCCTTTATGCACCGGACCTGTGATCAGCGCGGCAAATTCGCCGt                      <  2:935450/100‑1 (MQ=255)

                                                                            ctctTCGAAAAACTCGGTATGGCCGGTAAAAGGAATGCCAGCGTCGTTAATAACGCCTTTATGCACCGGACCTGTGATCAGCGCGGCAAATTCGCCGTTc                    >  1:2583955/1‑100 (MQ=255)

                                                                            ctctTCGAAAAACTCGGTATGGCCGGTAAAAGGAATGCCAGCGTCGTTAATAACGCCTTTATGCACCGGACCTGTGATCAGCGCGGCAAATTCGCCGTTc                    <  2:1468963/100‑1 (MQ=255)

                                                                            ctctTCGAAAAACTCGGTATGGCCGGTAAAAGGAATGCCAGCGTCGTTAATAACGCCTTTATGCACCGGACCTGTGATCAGCGCGGCAAATTCGCCGTTc                    >  2:1708407/1‑100 (MQ=255)

                                                                            ctctTCGAAAAACTCGGTATGGCCGGTAAAAGGAATGCCAGCGTCGTTAATAACGCCTTTATGCACCGGACCTGTGATCAGCGCGGCAAATTCGCCGTTc                    >  2:1155695/1‑100 (MQ=255)

                                                                            ctctTCGAAAAACTCGGTATGGCCGGTAAAAGGAATGCCAGCGTCGTTAATAACGCCTTTATGCACCGGACCTGTGATCAGCGAGGCAAATTCGACGTTc                    >  1:726190/1‑100 (MQ=255)

                                                                             tctTCGAAAAACTCGGTACGGCCGGTAAAAGGAATGCCAGCGTCGTTAATAACGCGTTAATGCAACGAACCTGTGATCAGCACAGCAAAATCGCCGTTc                    >  2:1628999/1‑99 (MQ=255)

                                                                             nctTCGAAAAACTCGGTATGGCCGGTAAAAGGAATGCCAGCGTCGTTAATAACGCCTTTATGTACCGGACCTGTGATCAGCGCGGCAAATTCGCCGTTCa                   >  1:328590/2‑100 (MQ=255)

                                                                              ctTCGAAAAACTCGGTATGGCCGGTAAAAGGAATGCCAGCGTCGTTAATAACGCCTTTATACACCGGACCTGTGATCAGCGCGGCAAATTCGCCGTTCAg                  >  1:976941/1‑100 (MQ=255)

                                                                                tCGAAAAACTCGGTATGGCCGGTAAAAGGAATGCCAGCGTCGTTAATAACGCCTTTATGCACCGGACCTGTGATCAGCGCGGCAAATTCGCCGTTCAGAc                <  2:549799/100‑1 (MQ=255)

                                                                                tCGAAAAACTCGGTATGGCCGGTAAAAGGAATGCCAGCGTCGTTAATAACGCCTTTATGCACCGGACCTGTGATCAGAGAGGCAAATTCGCCGTTCAGAc                >  1:1794543/1‑100 (MQ=255)

                                                                                  gAAAAACTCGGTATGGCCGGTAAAAGGAATGCCAGCGTCGTTAATAACGCCTTTATGCACCGGACCTGTGATCAGCGCGGCAAATTCGCCGTTCAGACaa              <  2:2904326/100‑1 (MQ=255)

                                                                                  gAAAAACTCGGTATGGCCGGTAAAAGGAATGCCAGCGTCGTTAATAACGCCTTTATGCACCGGACCTGTGATCAGCGCGGCAAATTCGCCGTTCAGACaa              >  2:1578409/1‑100 (MQ=255)

                                                                                    aaaaCTCGGTATGGCCGGTAAAAGGAATGCCAGCGTCGTTAATAACGCCTTTATGCACCGGACCTGTGATCAGCGCGGCAAATTCGCCGTTCAGACAAcc            <  2:1273007/100‑1 (MQ=255)

                                                                                       aCTCGGTATGGCCGGTAAAAGGAATGCCAGCGTCGTTAATAACGCCTTTATGCACCGGACCTGTGATCAGCGAGGCAAATTCGCCGTTCAGACAACCATc         >  1:997907/1‑100 (MQ=255)

                                                                                        cTCGGTATGGCCGGTAAAAGGAATGCCAGCGTCGTTAATAACGCCTTTATGCACCGGACCTGTGATCAGCGCGGCAAATTCGCCGTTCAGACAACCATCg        <  2:2915130/100‑1 (MQ=255)

                                                                                        cTCGGTATGGCCGGTAAAAGGAATGCCAGCGTCGTTAATAACGCCTTTATGCACCGGACCTGTGATCAGCGCGGCAAATTCGCCGTTCAGACAACCATCg        <  2:1665899/100‑1 (MQ=255)

                                                                                         tCGGTATGGCCGGTAAAAGGAATGCCAGCGTCGTTAATAACGCCTTTATGCACCGGACCTGTGATCAGCGCGGCAAATTCGCCGTTCAGACAACCATCGc       <  2:726186/100‑1 (MQ=255)

                                                                                          cGGTATGGCCGGTAAAAGGAATGCCAGCGTCGTTAATAACGCCTTTATGCACCGGACCTGTGATCAGCGCGGCAAATTCGCCGTTCAGACAACCATCGc       <  2:1441522/99‑1 (MQ=255)

                                                                                          cGGTATGGCCGGTAAAAGGAATGCCAGCGTCGTTAATAACGCCTTTATGCACCGGACCTGTGATCAGCGCGGCAAATTCGCCGTTCAGACAACCATCGCa      <  2:388310/100‑1 (MQ=255)

                                                                                            gTATGGCCGGTAAAAGGAATGCCAGCGTCGTTAATAACGCCTTTATGCACCGGACCTGTGAACAGCGCGGCAAATTTGCCGTTCAGACAACCATCGCAc     >  2:2620335/1‑99 (MQ=255)

                                                                                              aTGGCCGGTAAAAGGAATGCCAGCGTCGTTAATAACGCCTTTATGCACCGGACCTGTGATCAGCGCGGCAAATTCGCCGTTCAGACAACCATCGCACGCt  >  2:512344/1‑100 (MQ=255)

                                                                                              aTGGCCGGTAAAAGGAATGCCAGCGTCGTTAATAACGCCTTTATGCACCGGACCTGTGATCAGCGCGGCAAATTCGCCGTTCAGACAACCATCGCACGCt  <  1:858984/100‑1 (MQ=255)

GTAAATGCGTCGTTGCCAGCGCCACGCGAAGTTCTTCGGTCGCCAGCATCATCACCACCTTTTTCGCCTGCGAACGCTCTTCGAAAAACTCGGTATGACCGGTAAAAGGAATGCCAGCGTCGTTAATAACGCCTTTATGCACCGGACCTGTGATCAGCGCGGCAAATTCGCCGTTCAGACAACCATCGCACGCT  >  NC_000913/52915‑53108

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`