Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,708,410 T→A 100% intergenic (‑705/+206) dppA ← / ← proK dipeptide/heme ABC transporter periplasmic binding protein; dipeptide chemotaxis receptor/tRNA‑Pro

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,708,4100TA98.7% 333.7 / ‑4.1 112intergenic (‑705/+206)dppA/proKdipeptide/heme ABC transporter periplasmic binding protein; dipeptide chemotaxis receptor/tRNA‑Pro
Reads supporting (aligned to +/- strand):  ref base T (0/3);  new base A (56/53);  total (56/56)
Fisher's exact test for biased strand distribution p-value = 2.43e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 112 total aligned reads displayed.

GGCACATTTGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACGTCGAGTCGAACCTCCCTGCCCCCGGAGGTTCTCATCCTCATGAGCTGCAGATGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTACTCGCCACTTCGTGGCTCGCCCTGCGGGCCGTTGCTGGC  >  NC_000913/3708313‑3708506
                                                                                                 |                                                                                                
ggCACATTTGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACGTCGAGTCGAACCTCCCTGCCCCCGGAGGTTCTCATCCTCATGAGCTGCAGAAGc                                                                                                <  1:795849/100‑1 (MQ=255)
 gCACATTTGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACGTCGAGTCGAACCTCCCTGCCCCCGGAGGTTCTCATCCTCATGAGCTGCAGAAGCa                                                                                               >  1:2786600/1‑100 (MQ=255)
  cacaTTTGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACGTCGAGTCGAACCTCCCTGCCCCCGGAGGTTCTCATCCTCATGAGCTGCAGAAGCaa                                                                                              >  1:2959088/1‑100 (MQ=255)
  cacaTTTGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACGTCGAGTCGAACCTCCCTGCCCCCGGAGGTTCTCATCCTCATGAGCTGCAGAAGCaa                                                                                              >  1:2943340/1‑100 (MQ=255)
      tttGCCTGATGCGACGCTGACGCGTCTTATCAGGCTTACGTCGAGTCGAACCTCCCTGCCCCCGGAGGTTCTCATCCTCATGAGCTGCAGAAGCaaaaaa                                                                                          <  2:1561942/100‑1 (MQ=255)
      tttGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACGTCGAGTCGAACCTCCCTGCCCCCGGAGGTTCTCATCCTCATGAGCTGCAGAAGCaaaaaa                                                                                          <  1:2106256/100‑1 (MQ=255)
       ttGGCTGATGCGACGCTGACGCGTCTTATCAGGCCTACGTAGAGTCGAACCTCCCTGCACCCGGAGGTTCTTATCGTCATGAGCTGCAGAAGCaaaaaa                                                                                          <  2:2738534/99‑1 (MQ=21)
       ttGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACGTCGAGTCGAACCTCCCTGCCCCCGGAGGTTCTCATCCTCATGAGCTGCAGAAGCAAAAAAg                                                                                         <  1:1002100/100‑1 (MQ=255)
       ttGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACGTCGAGTCGAACCTCCCTGCCCCCGGAGGTTCTCATCCTCATGAGCTGCAGAAGCAAAAAAg                                                                                         <  1:2017955/100‑1 (MQ=255)
        tGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACGTCGAGTCGAACCTCCCTGCCCCCGGAGGTTCTCATCCTCATGAGCTGCAGAAGCAAAAAAGc                                                                                        >  1:2944772/1‑100 (MQ=255)
         gCCTGATGCGACGCTGACGCGTCTTATCAGGCCTATGTCGAGTCGAACCTCCCTGACCCCGGAGGTTCTCATCCTCATGAGCTGCAGAAGCAAAAAAGcc                                                                                       <  1:504504/100‑1 (MQ=255)
         gCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACGTCGAGTCGAACCTCCCTGCCCCCGGAGGTTCTCATCCTCATGAGCTGCAGAAGCAAAAAAGcc                                                                                       <  1:1014371/100‑1 (MQ=255)
         gCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACGTCGAGTCGAACCTCCCTGCCCCCGGAGGTTCTCATCCTCATGAGCTGCAGAAGCAAAAAAGcc                                                                                       >  1:510072/1‑100 (MQ=255)
         gCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACGTCGAGTCGAACCTCCCTGCCCCCGGAGGTTCTCATCCTCATGAGCTGCAGAAGCAAAAAAGcc                                                                                       <  2:1781803/100‑1 (MQ=255)
         gCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACGTCGAGTCGAACCTCCCTGCCCCCGGAGGTTCTCATCCTCATGAGCTGCAGAAGCAAAAAAGcc                                                                                       <  2:2494259/100‑1 (MQ=255)
               tGCGACGCTGACGCGTCTTATCAGGCCTACGTCGAGTCGAACCTCCCTGCCCCCGGAGGTTCTCATCCTCATGAACTGCAGAAGCAAAAAAGCCTGCt                                                                                   >  1:1457150/1‑98 (MQ=255)
                gCGACGCTGACGCGTCTTATCAGGCCTACGTCGAGTCGAACCTCCCTGCCCCCGGAGGTTCTCATCCTCATGAGCTGCAGAAGCAAAAAAGCCTGCt                                                                                   <  2:298815/97‑1 (MQ=255)
                 cGACGCTGACGCGTCTTATCAGGCCTACGTCGAGTCGAACCTCCCTGCCCCCGGAGGTTCTCATCCTCATGAGCTGCAGAAGCCAAAAAGCCTGCTcaac                                                                               >  2:2428065/1‑97 (MQ=255)
                  gACGCTGACGCGTCTTATCAGGCCTACGTCGAGTCGAACCTCCCTGCCCCCGGAGGTTCTCATCCTCATGAGCTGCAGAAGCAAAAAAGCCTGCTCGTTg                                                                              >  1:1830918/1‑100 (MQ=255)
                  gACGCTGACGCGTCTTATCAGGCCTACGTCGAGTCGAACCTCCCTGCCCCCGGAGGTTCTCATCCTCATGAGCTGCAGAAGCAAAAAAGCCTGCTCGTTg                                                                              <  2:485239/100‑1 (MQ=255)
                  gACGCTGACGCGTCTTATCAGGCCTACGTCGAGTCGAACCTCCCTGCCCCCGGAGGTTCTCATCCTCATGAGCTGCAGAAGCAAAAAAGCCTGCTCGTTg                                                                              >  2:1096515/1‑100 (MQ=255)
                  gACGCTGACGCGTCTTATCAGGCATACGTCGAGTCGAACCTCCCTGCCCCCGGAGGTTCTCATCCTCATGAGCTGCAGAAGCAAAAAAGCCTGCTCGTTg                                                                              <  2:1960263/100‑1 (MQ=255)
                   aCGCTGACGCGTCTTATCAGGCCTACGTCGAGTCGAACCTCCCTGCCCCCGGAGGTTCTCATCCTCATGAGCTGCAGAAGCAAAAAAGCCTGCTCGTTGa                                                                             <  2:2944740/100‑1 (MQ=255)
                   aCGCTGACGCGTCTTATCAGGCCTACGTCGAGTCGAACCTCCCTGCCCCCGGAGGTTCTCATCCTCATGAGCTGCAGAAGCAAAAAAGCCTGCTCGTTGa                                                                             <  1:2561699/100‑1 (MQ=255)
                   aCGCTGACGCGTCTTATCAGGCCTACGTCGAGTCGAACCTCCCTGCCCCCGGAGGTTCTCATCCTCATGAGATACAGAAGAAAAAAAGCCTGCTCGTTGa                                                                             <  2:2935364/100‑1 (MQ=18)
                    cGCTGACGCGTCTTATCAGGCCTACGTCGAGTCGAACCTCCCTGCCCCCGGCGGTTCTCATCCTCATGAGCTGCAGAAGCAAAAAAGCCTGCTCGTTGAg                                                                            >  2:2782269/1‑100 (MQ=255)
                    cGCTGACGCGTCTTATCAGGCCTACGTCGAGTCGAACCTCCCTGCCCCCGGAGGTTCTCATCCTCATGAGCTGCAGAAGCAAAAAAGCCTGCTCGTTGAg                                                                            <  2:2382954/100‑1 (MQ=255)
                    cGCTGACGCGTCTTATCAGGCCTACGTCGAGTCGAACCTCCCTGCCCCCGGAGGTTCTCATCCTCATGAGCTGCAGAAGCAAAAAAGCCTGATCATTGa                                                                             >  1:659200/1‑99 (MQ=255)
                     gCTGTCTCGTCTTATCAGGCGTACGTTGATTCGAATTTCCCTGCCCCCGGAGGTTCTCATCCTCATGAGCTGCAGAAGCAAAAAAGCCTGCTCGTTGAGc                                                                           <  1:1878344/100‑1 (MQ=17)
                     gCTGACGCGTCTTATCAGGCCTACGTCGAGTCGAACCTCCCTGCCCCCGGAGGTTCTCATCCTCATGAGCTGCAGAAGCAAAAAAGCCTGCTCGTTGAg                                                                            <  1:2245874/99‑1 (MQ=255)
                       taaCGCGTCTTATCAGGACTACGTCGAGTCGAACCTCCCTGCCCCCAGATTTTCTCACCCTCATGTGCTGCAGAAGCAAAAAAGCCTGCTCGTTGAg                                                                            <  2:2382999/95‑1 (MQ=17)
                       tGACGCGTCTTATCAGGCTTACGTCGAGTCGAACCTCCCTGCCCCCGGAGGTTCTCATCCTCATGAGCTGCAGAAGCAAAAAAGCCTGCTCGTTGAGCAg                                                                         <  1:2742689/100‑1 (MQ=255)
                       tGACGCGTCTTATCAGGCCTACGTCGAGTCGAACCTCCCTGCCCCCGGAGGTTCTCATCCTCATGAGCTGCAGAAGCAAAAAAGCCTGCTCGTTGAGCAg                                                                         <  1:2685646/100‑1 (MQ=255)
                       tGACGCGTCTTATCAGGCCTACGTCGAGTCGAACCTCCCTGCCCCCGGAGGTTCTCATCCTCATGAGCTGCAGAAGCAAAAAAGCCTGCTCGTTGAGCAg                                                                         <  1:822916/100‑1 (MQ=255)
                        gACGCGTCTTATCAGGCCTACGTCGAGTCGAACCTCCCTGCCCCCGGAGGTTCTCATCCTCATGAGCTGCAGAAGCAAAAAAGCCTGCTcaacgagcagg                                                                        <  2:3027882/100‑11 (MQ=17)
                        gACGCGTCTTATCAGGCCTACGTCGAGTCGAACCTCCCTGCCCCCGGAGGTTCTCATCCTCATGAGCTGCAGAAGCAAAAAAGCCTGCTCGTTGAGCAgg                                                                        >  1:2175637/1‑100 (MQ=255)
                          cgcgTCTTATCAGGCCTACGTCGAGTCGAACCTCCCTGCCCCCGGAGGTTCTCATCCTCATGAGCTGCAGAAGCAAAAAAGCCTGCTCGTTGAGCAGGCt                                                                      >  1:793754/1‑100 (MQ=255)
                           gcgTCTTATCAGGCCTACGTCGAGTCGAACCTCCCTGCCCCCGGAGGTTCTCATCCTCATGAGCTGCAGAAGCAAAAAAGCCTGCTCGTTGAGCAGGCt                                                                      >  2:990688/1‑99 (MQ=255)
                            cgTTTTATCAGGCCTACGTCGAGTCGAACCTCCCTGCCCCCGGAGGTTCTCATCCTCATGAGCTGCAGAAGCAAAAAAGCCTGCTCGTTGAGCAGGCttt                                                                    <  1:2114710/100‑1 (MQ=255)
                             gTCTTATCAGGCTTACGTCGAGTCGAACCTCCCTGCCCCCGTGGGTTCTCATCCTCATGAGCTGCAGAAGCAAAAAAGCCTGCTCAACGAGCAGGCttt                                                                    <  2:1227938/99‑1 (MQ=14)
                             gTCTTATCAGGCCTACGTCGAGTCGAACCTCCCTGCCCCCGGAGGTTCTCATCCTCATGAACAACAGAAGAAAAAAAGCCTGAACGTTGAACa                                                                          >  1:936697/1‑93 (MQ=17)
                              tCTTATCAGGCCTACGTCGAGTCGAACCTCCCTGCCCCCGGAGGTTCTCATCCTCATGAGCTGCAGAAGCAAAAAAGCCTGCTCAACGAGCAGGCttttt                                                                  <  2:2237100/100‑2 (MQ=17)
                               cTTATCAGGCCTACGTCGGGTCGAACCTCCCTGCCCCCGGAGGTTCTCATCCTCAGGGGCTGCAGAAGCAAAAAAGCCTGCTCGTTGAGCAGGCtttgc                                                                  <  2:2123129/99‑3 (MQ=17)
                               cTTATCAGGCCTACGTCGAGTCGAACCTCCCTGCCCCCGGAGGTTCTCATCCTCATGAGCTGCAGAAGCAAAAAAGCCTGCTCGTTGAGCAGGCatt                                                                    >  1:2207293/1‑97 (MQ=255)
                               cTTATCAGGCCTACGTCGAGTCGAACCTCCCTGCCCCCGGAGGTTCTCATCCTCATGAGCTGCAGAAGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCg                                                                 >  2:1286999/1‑100 (MQ=255)
                                 tATCAGGCCTACGTCGAGTCGAACCTCCCTGCCCCCGGAGGTTCTCATCCTCATGAGCTGCAGAAGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGaa                                                               <  2:793750/100‑1 (MQ=255)
                                 tATCAGGCCTACGTCGAGTCGAACCTCCCTGCCCCCGGAGGTTCTCATCCTCATGAGCTGCAGAAGCAAAAAAGCCTGCACGTTGAGCAGGCTTTTCGaa                                                               >  1:1238889/1‑100 (MQ=255)
                                  aTCAGGCCTACGTCGAGTCGAACCTCCCTGCCCCCGGAGGTTCTCATCCTCATGAGCTGCAGAAGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAAt                                                              <  1:6622/100‑1 (MQ=255)
                                  aTCAGGCCTACGTCGAGTCGAACCTCCCTGCCCCCGGAGGTTCTCATCCTCATGAGCTGCAGAAGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAAt                                                              >  1:2563987/1‑100 (MQ=255)
                                  aTCAGGCCTACGTCGAGTCGAACCTCCCTGCCCCCGGAGGTTCTCATCCTCATGAGCTGCAGAAGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAAt                                                              >  2:1461034/1‑100 (MQ=255)
                                   tCAGGCCTACGTCGAGTCGAACCTCCCTGCCCCCGGAGGTTCTCATCCTAGTGACCTGCAGAAGAAAAAAAGCCTGCTCGTTAAGCCGGCGTGTCg                                                                 >  2:113431/1‑96 (MQ=14)
                                   tCAGGCCTACGTCGAGTCGAACCTCCCTGCCCCCGGACGTTCTCATCCTCATGAGCTGCAGAAGCAAAAAAGCCTGCTCGTTGAGCAGGCTTATCGAAtt                                                             >  2:1622065/1‑100 (MQ=255)
                                    cAGGCCTACGTCTAGTCGAACCTACCTGCCCCCGGAAGGTCTCATCCTCATGAGCTGCAGAAGCAAAAAAGCCTGCTGGTTGAGCAGGCTTTTCGAAtt                                                             >  2:1747832/1‑99 (MQ=14)
                                      ggCCTACGTCGAGTCGAACCTCCCTGCCCCCGGAGGTTCTCATCCTCATGAGCTGCAGAAGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTgg                                                          >  2:1514109/1‑100 (MQ=255)
                                      ggCCTACGTCGAGTCGAACCTCCCTGCCCCCGGAGGTTCTCATCCTCATGAGCTGCAGAAGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTg                                                           >  2:1422830/1‑99 (MQ=255)
                                        ccTACGTCGAGTCGAACCTCCCTGCCCCCGGAGGTTCTCATCCTCATGAGCTGCAGAAGCAAAAAAGTCTGATCGTTGAGGAGGCGTTTCGAATTTggct                                                        >  2:903918/1‑98 (MQ=11)
                                          tACGTCGAGTCGAACCTCCCTGCCCCCGGAGTTTCTCATACTCATGAGCTACAGAAGAAAAAAAACCTGCTCGTTGAGCAGGATTTTCGAATTTGGTCg                                                       >  1:39188/1‑99 (MQ=11)
                                            cGTCGAGTCGAACCTCCCTAACCCCGGAGGTTCTCATCCTCATGAGCTGCAGAAGAAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTg                                                    >  2:1857750/1‑100 (MQ=11)
                                             gtcgagtcgaACCTCCCTGCCCCCGGAGGTTCTCATCCTCATGAGCTGCAGAAGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGa                                                   <  1:1517192/100‑1 (MQ=11)
                                                           cccTGCCCCCGGAGGTTCTCATCCTCATGAGCTGCAGAAGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTACTCg                                     >  2:321906/1‑100 (MQ=25)
                                                           cccTGCCCCCGGAGGTTCTCATCCTCATGAGCTGCAGAAGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTACTCg                                     <  2:912593/100‑1 (MQ=25)
                                                            ccTGCCCCCGGAGGTTCTCATCCTCATGAGCTGCAGATGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTACTCGc                                    <  2:940826/100‑1 (MQ=35)
                                                            ccTGCCCCCGGAGGTTCTCATCCTCATGAGCTGCAGAAGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTACTCGc                                    >  2:3044790/1‑100 (MQ=25)
                                                             gatctcccGGAGGTTCTCATCCTCATGAGCTGCAGAAGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTACTCGcc                                   <  1:2285177/95‑1 (MQ=255)
                                                             cTGCCCCCGGAGGTTCTCATCCTCATGAGCTGCAGAAGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTACTCGcc                                   <  1:2672719/100‑1 (MQ=255)
                                                                cccccGGAGGTTCTCATCCTCATGAGCTGCAGAAGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTACTCGCCACt                                >  1:844191/1‑100 (MQ=255)
                                                                 ccccGGAGGTTCTCATCCTCATGAGCTGCAGAAGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTACTCGCCACt                                >  1:1640603/1‑99 (MQ=255)
                                                                  cccGGAGGTTCTCATCCTCATGAGCTGCAGAAGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTACTCGCCagatc                              >  2:2285151/1‑96 (MQ=255)
                                                                  cccGGAGGTTCTCATCCTCATGAGCTGCAGAAGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTACTCGCCACTTc                              >  2:2214497/1‑100 (MQ=255)
                                                                  cccGGAGGTTCTCATCCTCATGAGCTGCAGAAGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTACTCGCCACTTc                              >  1:245428/1‑100 (MQ=255)
                                                                   ccGGATGTTCTTATCCTCATGAGCTGCAGAAGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTACTCGCCACTTCg                             <  1:1915033/100‑1 (MQ=255)
                                                                     ggAGGTTCTCATCCTCATGAGCTGCAGAAGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTACTCGCCACTTCGTg                           <  1:2955527/100‑1 (MQ=255)
                                                                     ggAGGTTCTCATCCTCATGAGCTGCAGAAGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTACTCGCCACTTCGTg                           <  2:2175614/100‑1 (MQ=255)
                                                                      gAGGTTCTCATCCTCATGAGCTGCAGAAGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTACTCGCCACTTCGTgg                          <  2:1626860/100‑1 (MQ=255)
                                                                       aGGTTCTCATCCTCATGAGCTGCAGAAGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTACTCGCCACTTCGTGGc                         <  2:241273/100‑1 (MQ=255)
                                                                        ggTTCTCATCCTCATGAGCTGCAGAAGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTACTCGCCACTTCGTGGCt                        >  2:2706545/1‑100 (MQ=255)
                                                                          ttCTCATCCTCATGAGCTGCAGAAGTAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTACTCGCCACTTCGTGGCTCg                      <  1:1500286/100‑1 (MQ=255)
                                                                          ttCTCATCCTCATGAGCTGCAGAAGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTACTCGCCACTTCGTGGCTCg                      <  1:1674424/100‑1 (MQ=255)
                                                                          ttCTCATCCTCATGAGCTGCAGAAGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTACTCGCCACTTCGTGGCTCg                      <  1:1514126/100‑1 (MQ=255)
                                                                           tctcATCCTCATGAGCTGCAGAAGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTACTCGCCACTTCGTGGCTCGc                     <  1:2154025/100‑1 (MQ=255)
                                                                                  cTCATGAGCTGCAGAAGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTACTCGCCACTTCGTGGCTCGCCCTGCgg              >  2:868723/1‑100 (MQ=255)
                                                                                  cTCATGAGCTGCAGAAGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTACTCGCCACTTCGTGGCTCGCCCTGCgg              >  1:1457771/1‑100 (MQ=255)
                                                                                  cTCATGAGCTGCAGAAGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTACTCGCCACTTCGTGGCTCGCCCTGCgg              >  1:974014/1‑100 (MQ=255)
                                                                                  cTCATGAGCTGCAGAAGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTACTAGCCACTTCGAGGCTCGCACTgag               >  1:96904/1‑97 (MQ=17)
                                                                                   taaTGAGCTGCAGAAGCAAAAAATTCTTCTTTTTGTGTAGGCTTTTGGAATTTGGTCGGTTATAGAGTTTTACTCGCCATTTCGTGGCTCGCCCTGCgg              <  2:678907/97‑1 (MQ=255)
                                                                                   tCATGAGCTGCAGAAGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGGTAGAGGATTACTCGCCACTTCGTGGCTCGCCCTGCggg             <  2:1864501/100‑1 (MQ=255)
                                                                                   tCATGAGCTGCAGAAGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTACTCGCCACTTCGTGGCTCGCCCTGCggg             >  2:2413911/1‑100 (MQ=255)
                                                                                   tCATGAGCTGCAGAAGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTACTCGCCACTTCGTGGCTCGCCCTGCg               >  1:1219309/1‑98 (MQ=255)
                                                                                    cATGAGCTGCAGAAGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTACTCGCCACTTCCTGGCTCTCCCTGccg              >  2:1150985/1‑96 (MQ=17)
                                                                                       gAGCTGCAGAAGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTACTCGCTACTACGCGGCTGGCCCATCGGGc            >  2:767593/1‑97 (MQ=14)
                                                                                         gCTGCAGAAGCAAAAAAGCCTGCTCGTTGAGCAGTCTTTTCGAATTTGGTCGGTGATAGAGGATTACTCGCCACTTCGTGGCTCGCCCTGCGGGCCGTTg       <  1:819351/100‑1 (MQ=255)
                                                                                         gCTGCAGAAGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTACTCGCCACTTCGTGGCTCGCCCTGCGGGCCGTTg       >  1:2947723/1‑100 (MQ=255)
                                                                                          cTGCAGAAGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTACTCGCCACTTCGTGGCTCGCCCTGCGGGCCGTTGc      <  2:1671564/100‑1 (MQ=255)
                                                                                           ttcAGATGCAAAAAAGCTTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTACTCGCCACTTCGTGGCTCGCCCTGCGGGCCGTTGc      <  1:672053/97‑1 (MQ=255)
                                                                                            gCAGAAGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTACTCGCCACTTCGTGGCTCGCCCTGCGGGCCGTTGCTg    >  1:1829331/1‑100 (MQ=255)
                                                                                            gCAGAAGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTACTCGCCACTTCGTGGCTCGCCCTGCGGGCCGTTGCTg    >  1:1925728/1‑100 (MQ=255)
                                                                                            gCAGAAGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTACTCGCCACTTCGTGGCTCGCCCTGCGGGCCGTTGCTg    >  1:2736732/1‑100 (MQ=255)
                                                                                            gCAGAAGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTACTCGCCACGTCCTGGCTGGCCCTGCGGGACGATGCt     >  2:2880015/1‑99 (MQ=17)
                                                                                            gCAGAAGAAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAAAGGATTACTCGCCACTTCGTGGCTCGCCCTGCGGGCCGTTGCTg    >  1:1587617/1‑100 (MQ=255)
                                                                                              aGAAGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTACTCGCCACTTCGTGGCTCGCCCTGCGGGCCGTTGCTGgc  >  1:2871019/1‑100 (MQ=255)
                                                                                                 |                                                                                                
GGCACATTTGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACGTCGAGTCGAACCTCCCTGCCCCCGGAGGTTCTCATCCTCATGAGCTGCAGATGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTACTCGCCACTTCGTGGCTCGCCCTGCGGGCCGTTGCTGGC  >  NC_000913/3708313‑3708506

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: